ASIA SKIN MICROBIOME 2.0 CONGRESS

24-25 September 2019S I N G A P O R E

www.global-engage .com

#MicrobiomeSeries

Co-Hosts

WARM WELCOME

ASIA SKIN MICROBIOME 2.0 CONGRESS 2019

We are pleased to host our Asia Skin Microbiome 2.0 Congress, a spin-off from our Asia’s Microbiome series that has been around since 2014. This event is set to bring skin microbiome experts to Singapore on the 24th and 25th of September, 2019.

Co-hosted with the Agency of Science, Technology and Research (A*STAR), Skin Research Institute of Singapore (SRIS) and Skin Research Society of Singapore (SRSS), this event will promote understanding and reciprocal benefits of the latest scientific and business developments in skin microbiome research. Attracting more than 150 experts from Asia, Europe and US, this 2-day event will highlight cutting edge research, commercialization, and business case studies through presentations and panel discussions, an exhibition filled with solution providers showcasing their products, and ample networking opportunities promoting interactions and business reach. This congress includes both scientific and commercial interests with topics ranging from host-microbe interaction, skin disease, emerging technologies, interventional and cosmetic advancements, and regulatory considerations. exhibition room filled with technology providers showcasing their technologies.

KIMBERLY KLINEAssociate Professor, Nanyang

Technology University, Singapore

EXPERT SPEAKERS Include:

SALOMÉ LEIBUNDGUT-LANDMANN

Associate Professor for Immunology, University of Zürich, Switzerland

BERNHARD PAETZOLDChief Scientific Officer, S-Biomedic, Belgium

• Over 100 regional and global skin microbiome researchers ranging from both scientific and industry backgrounds

• 20 expert presentations including a panel discussion• 7+ hours of dedicated networking time• Exhibition hall featuring cutting edge technology and solution

providers• Innovation Challenge*• Complimentary networking drinks reception

HIGHLIGHTS OF THE CONGRESS

• Skin health, wellbeing, and microbiome-associated skin disease• Skin immunology• Microbe-based therapies• Emerging technologies for skin microbiome research and innovation• Cosmetic vs Therapeutic regulatory considerations • Case studies • Academic-Commercial partnerships

SPECIFIC FOCUS AREAS

CONFIRMED SPEAKERS

NEELAM MUIZZUDIN President, Skin Clinical Research Consultants, USA

JOHN COMMON Principal Investigator, Skin Research Institute (SRIS), Singapore

KIMBERLY KLINE Associate Professor, Nanyang Technology University, Singapore

JANHAVI RAUT R&D Director, Human Microbiome Science & Technology Platform, Unilever, India

ERIC HUANGChair Professor, Department of Biomedical Sciences and Engineering, National Central University, Taiwan

THOMAS DAWSON, JRSenior Principal Investigator, Skin Research Institute of Singapore, A*Star

HUIYING LIAssociate Professor, Department of Molecular & Medical Pharmacology, University of California Los Angeles (UCLA), USA

BERNHARD PAETZOLDChief Scientific Officer, S-Biomedic, Belgium

GREG HILLEBRANDSenior Principal Scientist, Amway, USA

SHIGEFUMI OKAMOTOProfessor, Department of Clinical Laboratory Science, Kanazawa University, Japan

ANINDYA DASGUPTAWork Stream Leader, Human Microbiome Unilever, India

XU YAOProfessor and Chief, Division of Allergy and Rheumatology, Institute of Dermatology, Chinese Academy of Medical Sciences, China

SALOMÉ LEIBUNDGUT-LANDMANNAssociate Professor for Immunology, University of Zürich, Switzerland

WONHEE JUNGProfessor, Department of Systems Biology, Chung-Ang University, Korea

SAEKO NAKAJIMAAssistant professor, Department of Dermatology, Kyoto University Graduate School of Medicine, Japan

NIRANJAN NAGARAJANSenior Group Leader and Associate Director, Computational and Systems Biology, Genome Institute of Singapore (GIS)

ELIZABETH WUManager, Consumer Scientific Innovation Manager, Johnson & Johnson, USA

JENNIFER YAU WING KIDepartment of Paediatrics, The Chinese University of Hong Kong, Hong Kong

ANNIKA KRUEGERIan Frazer Laboratory, The University of Queensland Diamantina Institute, Australia

LI XI CECILIASenior Manager and Department Head of APAC Clinical Science, Johnson and Johnson Consumer, China

KATJA FISCHERGroup Leader, QIMR Berghofer Medical Research Institute, Australia

ASIA SKIN MICROBIOME 2.0 CONGRESS 2019

POSTER PRESENTATIONS

MAKING A POSTER PRESENTATIONPoster presentation sessions will take place in breaks and alongside the other breakout sessions of the conference. Your presentation will be displayed in a dedicated area, with the other accepted posters from industry and academic presenters. We also issue a poster eBook to all attendees with your full abstract in and can share your poster as a PDF after the meeting if you desire (optional). Whether looking for funding, employment opportunities or simply wanting to share your work with a like-minded and focused group, these are an excellent way to join the heart of this congress.

In order to present a poster at the congress you need to be registered as a delegate. Please note that there is limited space available and poster space is assigned on a first come first served basis (subject to checks and successful registration). We charge an admin fee of $50 USD to industry delegates to present, that goes towards the shared cost of providing the poster presentation area and display boards, guides etc. This fee is waived for those representing academic institutions and not for profit organisations.

EVENT SPONSORS

Co-Hosts

Media Partners

Exhibitors Supporter

CONGRESS SCHEDULE

Registration & Refreshments08:00-08:50

08:50-09:00 Global Engage Welcome Address and Morning Chair’s Opening Remarks: Thomas L Dawson, JR, Senior Principal Investigator, Skin Research Institute of Singapore, A*STAR and President, Skin Research Society, Singapore

DAY 1 TUESDAY 24TH SEPTEMBER 2019

KEYNOTE ADDRESS:GREG HILLEBRAND Senior Principal Scientist, Amway Corporation, USAThe Jekyll and Hyde Skin Microbiome: Good Bugs Behaving BadlyIn 1886, two years after the Scottish surgeon Sir Alexander Ogston discovered Staphylococcus aureus in the pus of surgical abscises, fellow Scot, Robert Louis Stevenson published his famous gothic novel, The Strange Case of Dr. Jekyll

and Mr. Hyde. The well-respected and community-minded Dr. Henry Jekyll fought with his alternate evil personality, Mr. Edward Hyde. Like Jekyll and Hyde, we know that our microbiome can also take on dual personalities. A so-called ‘good’ skin bug, like the universally commensal Staphylococcus epidermidis, can also be an opportunistic pathogen and cause serious life-threatening infections. Conversely, a ‘bad’ skin bug like Staphylococcus aureus, noted for its role in impetigo, boils and atopic dermatitis, colonizes 30% of the US population, most without issue. Exactly what causes ‘Jekyll bacteria’ to transform into ‘Hyde bacteria’ is multifactorial, with strain, host immunity, environment, and other factors at play. The transformation involves quorum sensing, biofilm formation, and a plethora of metabolic changes that ultimately results in acute and chronic skin inflammation. Preventing, inhibiting or even reversing the pathways involved in this transformation is an attractive new therapeutic strategy. I will teach about this fascinating area of bacterial biology as it relates to the skin microbiome and how all skin and personal care products should help keep our Dr. Jekyll bacteria from becoming Mr. Hyde.

Morning Refreshments / Poster Presentations10:25-11:25

09:00-09:3009:30-09:55

09:55-10:25

SOLUTION PROVIDER PRESENTATIONFor sponsorship opportunities contact Reuben Raj:

reuben@global-engage.com / +603 2117 5193

11:25-11:50

PATHOGENESIS / BIOFILM-RELATED CASE STUDIES

11:50-12:15

ASIA SKIN MICROBIOME 2.0 CONGRESS 2019

NIRANJAN NAGARAJAN Senior Group Leader and Associate Director, Computational and Systems Biology, Genome Institute of Singapore (GIS)Understanding the microbial basis of body odor in pre-pubescent children and teenagersEven though human sweat is odorless, bacterial growth and decomposition of specific odor precursors in it is believed to give rise to body odor in humans. While mechanisms of odor generation have been widely studied in adults, little is known for pre-pubescent children and teenagers who have distinct sweat composition from immature apocrine and sebaceous glands, but

are arguably more susceptible to the social and psychological impact of malodor. We integrated information from whole-microbiome analysis of multiple skin sites (underarm, neck and head) and multiple time points (1h and 8h after bath), analyzing 180 samples in total to perform the largest metagenome-wide association study to date on malodor. Significant positive correlations were observed between odor intensity and the relative abundance of Staphylococcus hominis, Staphylococcus epidermidis and Cutibacterium avidum, as well as negative correlation with Acinetobacter schindleri and Cutibacterium species. Metabolic pathway analysis highlighted the association of branched chain fatty acid and acetic acid production (sour odor) from enriched S. epidermidis (teen underarm) and S. hominis (child neck) enzymes, and sulfur production from Staphylococcus species (teen underarm) with odor intensity, in good agreement with observed odor characteristics in pre-pubescent children and teenagers. Experiments with cultures on human and artificial sweat confirmed the ability of S. hominis and S. epidermidis to independently produce malodor with distinct odor characteristics. These results showcase the power of skin metagenomics to study host-microbial co-metabolic interactions, identifying distinct pathways for odor generation from sweat in pre-pubescent children and teenagers, and highlighting key enzymatic targets for intervention.

KIMBERLY KLINEAssociate Professor, Nanyang Technology University, SingaporePathogenic mechanisms of Enterococcus faecalis within biofilm-associated wound infectionThe Gram-positive Enterococci are commensal inhabitants of the gastrointestinal tract, as well as opportunistic pathogens associated with endocarditis, urinary tract infections, and wound infection. Many Enterococcal infections are difficult to treat due to their multi-drug resistance, association with bacterial biofilms, and polymicrobial nature. The goal of our research is

to understand the molecular mechanisms by which Enterococcus faecalis interacts with other bacterial species and the host in the context of these polymicrobial, biofilm-associated infections. In this talk, I will present our latest research exploring how E. faecalis modulate the host immune response to create a hospitable environment for itself and co-infecting microbes and to promote chronic wound infections.

12:15-12:40

MICROBIAL ECOLOGY & METAGENOMICS OF THE SKIN MICROBIOME

KATJA FISCHERGroup Leader, QIMR Berghofer Medical Research Institute, AustraliaThe Impact of Scabies on the healthy Skin MicrobiotaWe hypothesize that in the tropics scabies plays a significant role in the establishment, proliferation and transmission of opportunistic, pathogenic bacteria and forms a critical precursor of skin-borne infection that can lead to severe illness. Whole metagenome analysis and 16S/ITS1 rRNA amplicon Illumina MiSeq sequencing were used as methods. In a longitudinal pilot

study using an experimental scabies porcine model a drastic shift from commensal to pathogenic staphylococci in the skin coincided with the onset of scabies infection. To investigate scabies infestations in humans we analysed metagenome data generated from mite and skin material collected from severe crusted scabies patients and samples from multiple body sites of large cohorts of patients with common scabies, located in North Australia, India and France. We present the diversity and dynamics of microbes associated with human scabies and define molecular mechanisms that underpin this synergy, to provide the basis for improved treatment and management strategies.

Invitation Out

CONGRESS SCHEDULE DAY 1 TUESDAY 24TH SEPTEMBER 2019

ASIA SKIN MICROBIOME 2.0 CONGRESS 2019

14:40-15:05

Afternoon / Poster Presentations15:30-16:15

SKIN INFLAMMATORY DISEASES

XU YAOProfessor and Chief, Division of Allergy and Rheumatology, Institute of Dermatology Chinese Academy of Medical Sciences, Nanjing, China A tryptophan metabolite of the skin microbiota attenuates inflammation in patients with atopic dermatitis through the aryl hydrocarbon receptorPrevious studies have revealed significant alterations in the skin microbiota of AD patients not only in diversity and

composition but also in function, and the tryptophan (Trp) metabolic pathway is attenuated in the skin microbiota of AD patients. In the present work, by using a gel-patch method, we found that the level of indole-3-aldehyde (IAId), an indole derivative of Trp catabolism, was significantly lower in lesional and non-lesional skin of AD patients than that of healthy individuals. IAId significantly attenuated the skin inflammation in MC903-induced AD-like dermatitis, and this effect was blocked by an AhR antagonist and abolished in AhR-null mice. Further, IAId was found to inhibit the MC903-induced expression of thymic stromal lymphopoietin (TSLP) in keratinocytes in vivo and in vitro, which was mediated by the binding of AhR to the TSLP promoter. Our study revealed that the skin microbiota play a significant functional role in the pathogenesis of AD.

15:05-15:30

HUIYING LIAssociate Professor, Department of Molecular & Medical Pharmacology, University of California Los Angeles (UCLA), USADissecting the human skin microbiome in health and diseaseThe human skin microbiome plays important roles in skin health and disease. However, bacterial population structure and diversity at the strain level, as well as the underlying molecular mechanisms of the microbiome in health and disease pathogenesis are often poorly understood. Using metagenomic and metatranscriptomic approaches, we compared the skin

microbiome at the strain level and at the transcriptional level between healthy skin and acne vulgaris, one of the most common skin diseases. We revealed that while the relative abundances of the dominant skin bacterium, Propionibacterium acnes, were similar, the population structure at the strain level was significantly different between the two cohorts. We also found that the transcriptional profiles of the skin microbiome were distinct in acne patients compared to healthy individuals. Our findings highlight the importance of strain level analysis of the human microbiome to define the role of commensals in health and disease and suggest a molecular mechanism of bacterial pathogenesis in acne.

14:00-14:40

PANEL DISCUSSION:Commercialisation of the Skin Microbiome Research

GREG HILLEBRAND Senior Principal Scientist, Amway Corporation, USA

NEELAM MUIZZUDIN (Chair)President, Skin Clinical Research Consultants, USA

THOMAS L. DAWSON, JRSenior Principal Investigator, Skin Research Institute of Singapore, A*STAR and President, Skin Research Society, Singapore

Lunch / Poster Presentations13:00-14:00

12:40-13:00

EARLY CAREER RESEARCHER:JENNIFER YAU WING KIDepartment of Paediatrics, The Chinese University of Hong Kong, Hong KongThe role of skin microbiome in paediatric atopic dermatitisAtopic dermatitis (AD) is a prevalent childhood allergy around the globe. Although dysbiosis of skin microbiota was shown to take part in the disease pathogenesis, our understanding on microbiome was mainly sourced from the

amplicon sequencing data on bacteria. Our goal is to also identify multi-kingdom species resided on skin in the paediatric population using whole-genome shotgun sequencing and delineate their roles on childhood AD. We conducted a cross-sectional study, recruiting a hundred subjects, and collected control, non-lesional and lesional skin swabs for their microbial signatures. Several unreported non-Staphyloccocus bacterial species, fungal and viral members were identified to be associated with childhood AD. They were key contributors of lipid metabolism, antimicrobial biosynthesis and amino acid metabolisms at healthy state. A shift of contributors responsible for these core pathways during AD, implies possible mechanisms of how the commensals influence the epidermal barrier, modulate host immunity and interact with one another on the skin.

Chair: Niranjan Nagarajan, Senior Group Leader and Associate Director, Computational and Systems Biology, Genome Institute of Singapore (GIS)

CONGRESS SCHEDULE DAY 1 TUESDAY 24TH SEPTEMBER 2019

Chair's Closing Remarks / End of Day One17:05

JOHN COMMON Principal Investigator, Skin Research Institute of Singapore (SRIS), A*STAR, SingaporeSkin microbiome signatures in health and disease correlate with host immunity and microbial virulenceThe skin is a challenging ecosystem to study meta-omics due to the low amount of biomass that can be recovered, which limits downstream techniques that are currently feasible on human subjects. We have recently been using metagenomics to investigate microbial communities present on the skin of atopic dermatitis patients to better understand shifts in

community diversity and microbial functional characteristics. We can identify skin microbiome dermotypes that stratify groups of AD patients and observed that these groups correlate with host immunity and microbial virulence.

16:40-17:05

SAEKO NAKAJIMAAssistant professor, Department of Dermatology, Kyoto University Graduate School of Medicine, JapanAdaptive immunity to commensal skin fungi promotes inflammatory responsesSince the precise role of cutaneous microbiota in the control or promotion of skin inflammatory conditions remains unclear, we aimed to address how defined skin microbes could affect the development of psoriasis by using the imiquimod-induced psoriasis-like inflammation in mice associated with skin commensals. Candida albicansexacerbated epidermal

thickness and neutrophil infiltration. Such enhanced inflammation was not associated with skin fungal invasion and but was characterized by a dramatic influx of IL-17A producing CD4effector T cells into the skin. Of note, the Th17 cells accumulating in the lesions were C.albicansspecific. In addition, adaptive response to C.albicanstogether with imiquimod significantly enhanced Neutrophil extracellular traps (NETs) formation and in vivo inhibition of NET formation was associated with significant reduction in disease severity. Together, our result show that adaptive response to skin commensal fungi can have a dramatic impact on the severity of skin inflammatory disorder.

16:15-16:40

CONGRESS SCHEDULE

Refreshments08:00-08:55

08:55-09:00 Morning Chair: Greg Hillebrand, Senior Principal Scientist, Amway Corporation, USA

DAY 2 WEDNESDAY 25TH SEPTEMBER 2019

Morning Refreshments / Poster Presentations10:20-11:05

09:00-09:30

ASIA SKIN MICROBIOME 2.0 CONGRESS 2019

KEYNOTE ADDRESS:SALOMÉ LEIBUNDGUT-LANDMANN Associate Professor for Immunology, Vetsuisse Faculty, University of Zürich, SwitzerlandThe skin commensal yeast Malassezia at the interface of health and diseaseCommensal fungi of the mammalian skin, such as those of the genus Malassezia, are associated with atopic dermatitis and other common inflammatory skin disorders. Understanding of the causative relationship between fungal commensalism

and disease manifestation remains incomplete. By developing a murine epicutaneous infection model, we found Malassezia spp. selectively induce IL-17 and related cytokines. This response is key in preventing fungal overgrowth on the skin, as disruption of the IL-23/IL-17 axis compromises Malassezia-specific cutaneous immunity. Under conditions of impaired skin integrity, mimicking a hallmark of atopic dermatitis, the presence of Malassezia dramatically aggravates cutaneous inflammation, which again was IL-23- and IL-17-dependent. Consistently, we found a CCR6+ Th17 subset of memory T cells to be Malassezia-specific in both healthy individuals and atopic dermatitis patients, whereby the latter showed enhanced frequency of these cells. Thus, the Malassezia-induced type 17 response is pivotal in orchestrating antifungal immunity and in actively promoting skin inflammation.

WONHEE JUNG Professor, Department of Systems Biology, Chung-Ang University, KoreaGenomic analysis of antifungal resistance of the dandruff-associated skin fungusWhile the human gut microbiome Malassezia restricta is an opportunistic fungal pathogen on human skin and is associated with various skin diseases including seborrheic dermatitis, dandruff and atopic dermatitis. Among the 17 identified Malassezia species, M. restricta is the predominant species on human skin and is particularly associated with

dandruff, as suggested by recent large-scale mycobiome analyses. In this presentation, I will introduce our recent study on understanding the mechanism of ketoconazole resistance in clinically isolated M. restricta strains from dandruff patients. Comparative genome and transcriptome analyses were carried out, and the results were compared to that of a ketoconazole susceptible reference M. restricta strain. The results of our study suggest that genomic rearrangement, in particular, multiplication of locus encoding genes involved in drug resistance, is a common mechanism of ketoconazole resistance in M. restricta.

09:30-09:55

THOMAS L. DAWSON, JRSenior Principal Investigator, Skin Research Institute of Singapore, A*STAR and President, Skin Research Society, Singapore The Cutaneous Mycobiome and its Role in Skin Disease: Malassezia, friend or foe? While the human gut microbiome has realized virtual celebrity status amongst both the popular and scientific press, the skin mycobiome remains unexplored, elusive, and poorly understood. Only recently have investigations begun to focus on skin fungi, the majority to date primarily focused on bacteria via 16S sequencing or metagenomics without the read depth necessary to

identify fungi beyond the class level. Every human being’s skin is occupied by fungi, and the vast majority of people will be affected by a fungal-associated disease at some point during their lifetime. Multiple studies indicate a likely causative role for fungi in common skin disorders such as pityariasis versicolor and seborrheic dermatitis, and a role in exacerbation of many others including chronic wounds, atopic dermatitis, atopic eczema, and psoriasis. In contrast, several recent studies have indicated a potential protective role for skin fungi, from the reduction in skin disease associated with puberty to atopic dermatitis. We have spent more than 20 years defining the role of fungi in seborrheic dermatitis, including definition of the causal species, identification of a pathogenic mechanism, and clinical proof of concept validating one pathway, lipase mediated inflammation.

09:55-10:20

SHIGEFUMI OKAMOTOProfessor, Department of Clinical Laboratory Science, Division of Health Sciences, Kanazawa University, JapanComparison of skin microbiome between bedridden elderly with high risk of skin injury and healthy people with low risk of skin injuryBedridden elderly has a risk of skin injury including skin infectious diseases and skin inflammation. However, there have been no insights into how bedridden elderly show different skin microbiome in comparison with the age-matched ambulatory adults.

Therefore, we compared the skin microbiome and skin physiological functions of bedridden elderly with those of ambulatory elderly and young individuals. In our study, we determined higher gut-related bacteria, less commensals, higher skin pH, and lower transepidermal water loss on the skin of bedridden older patients compared with that of young or ambulatory older people. We additionally observed the propagation of skin bacteria with higher abundance of pathogens. In summary, we showed that the skin microbiome and skin physiological functions of bedridden elderly were changed compared to those of healthy participants, and the changes may be cause to the risk of skin injury in bed hidden elderly.

11:30-11:55

MALASSEZIA

11:05-11:30ELIZABETH WUManager, Consumer Scientific Innovation Manager, Johnson & Johnson, USALI XI CECILIASenior Manager and Department Head of APAC Clinical Science, Johnson and Johnson Consumer, ChinaResearch and partnerships to further our understanding of the skin microbiome and drive skin health solutions

The microbiome is emerging as relevant along the entire consumer journey through health and disease, and we at Johnson & Johnson Consumer franchises are excited to work in this space, in association with digestive health, oral health, and skincare. In this presentation, we will share some of our clinical research on advancing the understanding of the Chinese skin microbiome, including how cutaneous microbial distribution at different body sites interact with the topographic skin environment. We will also discuss on skin microbiome diversity profiling in dry skin and atopic dermatitis (lesional & non-lesional), and how it has guided our approach in finding technology solutions for those consumers. In addition to advancing the science of microbiome via internal research, we will share how Johnson & Johnson seeks to build collaborations with external partners to accelerate transformational healthcare solutions for our consumers.

Chair: Huiying Li, Associate Professor, Department of Molecular & Medical Pharmacology, University of California Los Angeles (UCLA), USA

ANINDYA DASGUPTAWork Stream Leader, Human Microbiome Unilever, IndiaGlycerol as a Prebiotic for SkinThe skin microbiome can be thought of as a living protective layer that partners with the skin to keep it healthy and in good condition; from preventing colonization of pathogens to supporting the immune system, to helping maintain skin’s normal pH, to the indirect benefits that come from metabolizing various compounds present in the skin. Following these reasons, the use of

prebiotics as a source of food for the skin commensal bacteria is an attractive route to enhance and activate the skin commensals to do the above more efficiently. We have used glycerol, which is commonly used in skin care products, in our work to show that it has prebiotic effects. Glycerol is metabolized by skin resident bacteria releasing fermentation by-products, such as short chain fatty acids and other organic acids. While SCFAs are antimicrobial in nature and prevent colonization by invading pathogens, lactic acid is an alpha-hydroxy acid that has further benefits for skin health. This includes improving barrier function of epidermis and the firmness and elasticity/suppleness of skin, as well as stimulating the turnover of dead skin cells. Use of glycerol and other prebiotics is thus an effective way towards a healthier and more resilient skin.

CONGRESS SCHEDULE DAY 2 WEDNESDAY 25TH SEPTEMBER 2019

Lunch / Poster Presentations / One-to-One Meetings13:00-14:00

THERAPEUTIC STRATEGIES FOR THE SKIN MICROBIOME

BERNHARD PAETZOLD Chief Scientific Officer, S-Biomedic, BelgiumCutibacterium acnes the main component of the skin microbiotaC. acnes is the main bacterial component of the skin microbiome on sebaceous and dry body sites (Byrd et al. 2018). Despite its dominant role on the skin, many skin treatments aim at eradicating this bacterial species. I will present a selection of examples of the literature why this bacteria is important to us and how it has a symbiotic relationship with its human host.

14:00-14:2514:25-14:50

12:20-12:40

EARLY CAREER RESEARCHER:ANNIKA KRUEGERIan Frazer Laboratory, The University of Queensland Diamantina Institute, AustraliaStaphylococcus aureus secreted proteins as local regulators of epithelial inflammation in photo-damaged skinIncreasing evidence shows that microbial secretions are able to modulate local immune responses in epithelial cells and thus can play a key role in inflammatory disease, tumour development and progression. Premalignant actinic keratosis (AK) and

squamous cell carcinoma (SCC) skin lesions are associated with a higher abundance of the gram-positive bacterium Staphylococcus aureus, however, the direct impact of increased bacterial colonization on the lesion microenvironment and progression is unknown. We hypothesise that S. aureus secreted products can induce chronic cutaneous inflammation and thus contribute to disease progression from AK to SCC. The current study aims to identify bioactive compounds with immunomodulatory and potentially pro-tumorigenic properties secreted by skin lesion-associated S. aureus. The proteomic content of sterile culture supernatants from 31 clinical S. aureus strains isolated from control and lesional skin swabs was determined via mass spectrometry (MS). Cultured human keratinocytes were stimulated with MS-defined S. aureus supernatants and subsequent cytokine responses measured. We found a specific S. aureus toxin secretory profile to correspond to increased keratinocyte pro-inflammatory responses in vitro. Further, S. aureus secretions were found to stimulate proliferation in cultured keratinocytes. Current experiments investigate whether pro-inflammatory and -proliferative toxins can influence skin carcinogenesis in an in vivo mouse model.

JANHAVI RAUTR&D Director, Human Microbiome Science & Technology Platform, Unilever, India Role of skin-microenvironment in shaping the MicrobiomeIt is well recognized today that the skin microbiome and the host skin form a joint ecosystem, the health of which is governed by a balanced microbiome functioning in harmony with the host. The dynamics of this ecosystem is governed by a complex set of microbe-microbe and microbe-host interactions. Product usage and the resultant host micro-environment

play an important role in further modulating these interactions. The nature of these interactions could be two-body or many-body in nature leading to emergent effects that cannot be predicted a priori. We have developed multipronged approach to combining microbiomics, in vitro models to study the role of substrate microenvironment, in silico approaches for microbial assemblies. We will discuss a few examples of this approach and its applications towards understanding the skin microbiome and relevant endpoints of skin health.

11:55-12:20

POSTER WINNERS TALK

12:40-13:0014:50-15:20

Conference Close15:20

CLOSING KEYNOTE ADDRESS:CHUN-MING ERIC HUANGChair Professor, Department of Biomedical Sciences and Engineering, National Central University, TaiwanNovel probiotics from electricity-producing skin microbes for microbiome editingOur recent results have demonstrated that skin bacteria can yield electricity during the bacterial fermentation. By using electrogenic bacteria, we develop new technology derived from the concept of probiotic-prebiotic-postbiotic-

“electrobiotic”. Next-generation sequencing (NGS), although it is a new approach to biomarker identification, may not be able to dynamically detect the dysbiotic microbiome. We here introduce the technology of ”electrobiotic” for profiling and monitoring the skin dysbiosis in real time. Most importantly, these electrogenic skin bacteria will become novel probiotics for treatments of human diseases. For example, electrons produced by electrogenic bacteria can function as antioxidants for reduction of ultraviolet (UV)-induced skin damages. Furthermore, the electrogenic skin bacteria may affect the human mentality by regulation of brain waves. In this talk, the novel probiotics using electrogenic bacteria will be introduced. The application of these electrogenic probiotics for microbiome editing and banking as well as treatments of human diseases will be highlighted.

Chair: Niranjan Nagarajan, Senior Group Leader and Associate Director, Computational and Systems Biology, Genome Institute of Singapore (GIS)

ASIA SKIN MICROBIOME 2.0 CONGRESS 2019

VENUE INFORMATION

ASIA SKIN MICROBIOME 2.0 CONGRESS 2019

Hotel Fort Canning,11 Canning Walk, Singapore, 178881www.hfcsingapore.com

Hotel Fort Canning is a magnificent and award-winning conservation hotel tucked within 18 hectares of lush greenery of Fort Canning Park. The award-wining boutique hotel is luxurious and trendy,and it combines the romance of a grand colonial edifice with lush green parklands in the heart of the city. Hotel Fort Canning was styled by the award-winning DP Architects to incorporate the finest hospitality amenities,while retaining and conserving its old-style, colonial glamour. Today, the hotel serves as one of the finest boutique hotels Singapore has to offer. It straddles the Orchard Road shopping belt, the Clarke Quay entertainment hub, the Central Business District and the Civic District.