Appendix ALab Floor Plan

1Foot

A

21'4"

10'7''

Refrig.

A - 100psi Pressure Outlet + dry filterelectrical outlets 120vdimmer switch with LED LightingComputer LAN drop

Shelving (1ft)

A

Sheath Bag Hooks

v.150306

Lam

inar

H

ood

(BS

C II

)

keyed Access Pad

Rm.5613

Rm.5613-B

Rm.5609

Rm.5613-A

Glass wall / covered by dry wall

Ball-in-the-wall®

Appendix BFlow Lab Warning

(Active and Inactive Sort)

Caution(:(Potentially(Dangerous(Aerosol(Exposure(DO(NOT(ENTER(IF(SORTING(IS(ACTIVE(

Authorized(Personnel(ONLY(Flow(Cytometry(Core(Cell(Sorting(Facility

Active(Sort

Caution(:(Potentially(Dangerous(Aerosol(Exposure(DO(NOT(ENTER(IF(SORTING(IS(ACTIVE(

Authorized(Personnel(ONLY(Flow(Cytometry(Core(Cell(Sorting(Facility

Inactive(Sort

Appendix CAerosol System Flow

Vacuum Ports

Negative Pressure Flow

Vacuum Gauge

Pump Monitor

HEPA Filter and Vacuum Pump

Aerosol Management System

Appendix DSort Chamber Modes

Standby mode Active mode

Appendix ECyClex-d Device

Location

Appendix FCyclex-d Device

Figure A

Figure B

Appendix GDragon Green Particles

0 102 103 104 105B515-A

0

102

103

104

105

SSC

-A

0 102 103 104 105B515-A

0

2000

4000

6000

8000

10K

# C

ells

Dragon Green Beads

Appendix HSalt Crystals on a Collection

Slide with Dragon Green Beads

Appendix IAerosol Containment

Report

Appendix JSheath Filter

A

C

B

Appendix KPAPR system

Tyvek Body Suit

HEPA Shroud

Double Gloves

AirMAX HEPA filter PAPR

Helmet

Battery Light Level

Suit Boots

Suit Face Shield

Battery Pack

Appendix LRecord and Checklist