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APPENDICES Table 1. Dissolved oxygen content and water quality relationship DO Content (ppm O 2 ), 20C Water Quality 8-9 Clean, good water 7-11 ppm: ideal for stream fishes including cold water fishes 6.7 - 7.9 Slightly polluted 4.5 - 6.6 Moderately polluted, can sustain life of warm water fishes Below 4.5 Highly polluted 0 - 2 Cannot sustain life Molarity of Na 2 S 2 O 3 : ( weight of standard KIO 3 )( %purity )( 6 molesthiosulfate 1 moleiodate ) ( MW thiosulfate )( volumetitrant) Trial Molarity 1 0.01277260316 2 0.01281130802 3 0.01273413146 Average 0.01277268088 DO Content: ppmoxygen= ( Mthiosulfate )( vol thiosulfate )( 1 mole oxygen 4 moles thiosulfate )( MW oxygen) volsample ( L) Trial DO content (ppm O 2 ) 1 5.924301482 2 5.515728966 3 5.720015224 Average 5.720015224 Statistical Calculations: Our group’s data Other group’s data Trial DO content (ppm) Trial DO content (ppm) 1 5.924301482 1 5.6 2 5.515728966 2 5.628493504

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APPENDICES

Table 1. Dissolved oxygen content and water quality relationship

DO Content (ppm O2), 20C Water Quality8-9 Clean, good water 7-11 ppm: ideal for stream fishes

including cold water fishes6.7 - 7.9 Slightly polluted4.5 - 6.6 Moderately polluted, can sustain life of warm water fishes

Below 4.5 Highly polluted0 - 2 Cannot sustain life

Molarity of Na2S2O3:

(weight of standard KIO 3)(%purity)( 6moles thiosulfate1mole iodate

)

(MW thiosulfate)(volumetitrant )

Trial Molarity1 0.012772603162 0.012811308023 0.01273413146

Average 0.01277268088DO Content:

ppmoxygen=(M thiosulfate)(vol thiosulfate)( 1moleoxygen

4moles thiosulfate)(MW oxygen)

vol sample (L)

Trial DO content (ppm O2)1 5.9243014822 5.5157289663 5.720015224

Average 5.720015224Statistical Calculations:

Our group’s data Other group’s dataTrial DO content (ppm) Trial DO content (ppm)

1 5.924301482 1 5.62 5.515728966 2 5.6284935043 5.720015224

Mean 5.720015224 Mean 5.6142Standard deviation 0.204286258 Standard deviation 0.0201479499

RSD 35.71428571 RSD 3.588748156CL 5.7200±0.5071 CL 5.6142±0.1809

Range 0.408572516 Range 0.028493504