Anznmf 2016 Abstract Book

8/19/2019 Anznmf 2016 Abstract Book

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These abstracts should not be cited in bibliographies. Material contained herein should be treated aspersonal communication and should be cited as such only with the consent of the author.

Sponsors

ANFF-Q, ANFF-SA and Queensland Micro and Nanotechnology Centre (QMNC).


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Abstracts of papers presented at the

7TH AUSTRALIA AND NEW ZEALAND NANO-MICROFLUIDICSSYMPOSIUM (ANZNMF 2016)

Griffith University EcoCentre, Nathan Campus, 21-23 March 2016

SYMPOSIUM CO-CHAIRS

Dr Muhammad J. A. Shiddiky, Griffith University Professor Nam-Trung Nguyen, Griffith University

LOCAL ORGANISING COMMITTEE

Mr Nazmul Islam, Ms Sharda Yadav, Dr Say Hwa Tan,Dr Muhammad J. A. Shiddiky and

Professor Nam-Trung Nguyen

SCIENTIFIC ADVISORSProfessor Michael Breadmore, University of Tasmania

Professor Leslie Yeo, RMIT University Dr Craig Priest, University of South Australia

Professor Amanda V. Ellis, Flinders University Professor Stephen J. Haswell, Deakin University Dr Geoff R. Willmott, The University of Auckland


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AUSTRALIA AND NEW ZEALANDNANO-MICROFLUIDICS SYMPOSIUM(ANZNMF)

The ANZNMF meeting is the annual gathering of the ANZ

Microfluidics community, which is an informal network of

researchers working in the field in Australia and New Zealand with

the aim of facilitating exchange of ideas and collaborative

interactions. The network was inaugurated at the first ANZNMF

meeting in Melbourne at Monash University in 2009. Successive

meetings were hosted by University of New South Wales (UNSW)

in Sydney (2011), the MacDiarmid Institute in Wellington (2012),

University of South Australia and Flinders University in Adelaide

(2013), University of Tasmania in Hobart (2014) and RMIT

University, Deakin University and the Melbourne Centre for

Nanofabrication in Melbourne (2015).


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PLENARY SPEAKERS

Professor Carolyn RenMechanical and Mechatronics Engineering; Canada Research Chair in DropletMicrofluidics and Lab-on-a-Chip (LOC) Technology

Dr. Ren received her Ph.D. in Mechanical Engineering from the University of Toronto in2004 and Master’s and Bachelor’s degrees in Thermal Engineering from Harbin Instituteof Technology in 1995 and 1992, separately. She worked in Dalian University ofTechnology for four years prior to her Ph.D. study in Canada. She joined the University of

Waterloo (UW) in May 2004 as an Assistant Professor and promoted to Associate and Full Professor in 2010and 2015, respectively. She has received many research awards including CSME fellow in 2012, ResearchExcellence Award from UW in 2010, Canada Research Chair in Lab-on-a-Chip Technology in 2009 and2014, and Early Research Award from the Ministry of Research and Innovation of Ontario in 2007.

Professor Leslie Yeo Civil, Environmental and Chemical EngineeringRMIT University, Melbourne

Leslie Yeo is currently an Australian Research Council Future Fellow and Professorof Chemical Engineering at RMIT University, Australia. He received his PhD fromImperial College London in 2002, for which he was awarded the Dudley Newitt prize

for a computational/theoretical thesis of outstanding merit. Prior to joining RMITUniversity, he was a postdoctoral research associate in the Department of Chemical & BiomolecularEngineering at the University of Notre Dame, USA, after which he held a faculty position at Monash University.Dr Yeo was the recipient of the 2007 Young Tall Poppy Science Award from the Australian Institute for Policy& Science ‘in recognition of the achievements of outstanding young researchers in the sciences includingphysical, biomedical, applied sciences, engineering and technology’, and both the Dean and Vice-Chancellor’sawards for excellence in early career research at Monash University. Dr Yeo is co-author of the bookElectrokinetically Driven Microfluidics & Nanofluidics (Cambridge University Press), and the author of over 150research publications and 20 patent applications. He is also the Editor of the American Institute of Physics

journal Biomicrofluidics, editorial board member of Interfacial Phenomena & Heat Transfer and ScientificReports, and an Adjunct Senior Research Fellow in the Department of Physiology at Monash University.


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KEYNOTE SPEAKERS

Professor Justin J. Cooper-White University of Queensland (UQ) and CSIRO, AustraliaProfessor, Australian Institute for Bioengineering and Nanotechnology and School ofChemical Engineering, UQ,CSIRO OCE Science Leader,Director, Australian National Fabrication Facility – Queensland Node.

Professor Justin Cooper-White is a globally acknowledged pioneer in regenerativemedicine research. He leads two world-class research laboratories in Australia, the Tissue Engineering andMicrofluidics (TEaM) laboratory within The University of Queensland’s Australian Institute for Bioengineeringand Nanotechnology (AIBN), and the Biomaterials Discovery laboratory within the Manufacturing Flagship atCSIRO in Melbourne. His team of over 20 researchers focus on developing novel biomaterials, nanoparticles,scaffolds and engineered microfluidic devices and systems that enable endogenous tissue repair at sites of

damage and the safe expansion, differentiation and delivery of stem cells for regenerative medicineapplications. He currently holds the positions of Group Leader within the Australian Institute for Bioengineeringand Nanotechnology (AIBN, UQ), Professor of Bioengineering within the School of Chemical Engineering(UQ), CSIRO Office of the Chief Executive Science Leader (Manufacturing Flagship, Melbourne), FoundingDirector of the Queensland Node of the Australian National Fabrication Facility (ANFF-Q), and Professor andGroup Leader (Adjunct) within the Australian Regenerative Medicine Institute (ARMI, Monash University). Prof.Cooper-White has over 200 refereed publications cited >5000 times. He has also produced 6 Worldwidepatents that have reached National Phase Entry in USA, Europe and Australia. He is Past President of

Australasian Society for Biomaterials and Tissue Engineering (ASBTE) (2006-2008), and the AustralasianSociety of Rheology (2002-2004), and currently an Australian representative on the Int. Union of Societies forBiomaterials Science and Engineering (2012-current) and President of the Asian Biomaterials Federation andCouncil (2015-current).

Professor V. Amanda Ellis ARC Future FellowshipSchool of Chemical & Physical SciencesFlinders University

Prof. Ellis graduated from her PhD in 2003 at the University of Technology, Sydney.She undertook two US postdocs, the first at Rensselaer Polytechnic Institute (RPI) and

the second at New Mexico State University. She was then awarded a prestigious (1 of 16) New ZealandFoundation of Research Science and Technology (NZFRST) fellowship at Industrial Research Ltd, NZ (nowCallaghan Innovations) where she worked on microfluidics, in particular switchable surfaces and carbonnanotubes electrodes in microchannels. She currently holds an Australian Research Council Future Fellowshipat Flinders University and has over 125 publications with 2300 citations. Her work primarily involves themodification of surfaces for applications in microfluidics, desalination, forensic science and biosensing.

http://www.flinders.edu.au/search/?q=School+of+Chemical+&+Physical+Scienceshttp://www.flinders.edu.au/search/?q=School+of+Chemical+&+Physical+Sciences


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Professor Stephen HaswellChair in Sensors and MicrofluidicsCentre Regional & Rural FutureDeakin University

Stephen Haswell has spent the last thirty years of his career in the UK higher educationsector but recently moved from being Professor of Analytical Chemistry at the Universityof Hull, a post he has held for the past 23 years, to a new position in the Centre forRegional and Rural Futures at Deakin University as Professor in Sensors and

Microfluidics. Whilst Steve’s past research interests have been in the fields of elemental speciation,chemometrics and process analysis his research over the past 20 years have been in the areas of micro-reactors and Lab-on-a-chip technology. At Deakin Steve is establishing a world leading multidisciplinarytranslational research centre for Lab-on-a-chip technology, which will be capable of meeting the demandingneeds of industry and society for adaptive relevant technology in the areas of health, environment andagriculture.

Professor Spas D KolevProfessor of Chemistry, School of Chemistry Program Leader , Novel Chemistry Research, Centre for Aquatic PollutionIdentification and Management (CAPIM), Melbourne University

Spas Kolev is Professor of Chemistry of The University of Melbourne (Australia). Mostof his research is focused in the areas of flow analysis techniques, including paper-

based microfluidics; chemical sensors, development of polymer inclusion membranes for separation orsynthesis of metallic nanoparticles; and phytoremediation and phytomining. He has published close to 170

refereed articles, 3 book chapters and has co-edited a book entitled ‘ Advances in Flow Injection Analysis andRelated Techniques’ (Elsevier, 2008). He has given over 35 invited, keynote or plenary lectures at internationalconferences.

In recognition of his outstanding contributions to his fields of research, Spas Kolev has been awarded theRonald Belcher Memorial Award (Talanta, 1988), the Lloyd Smythe Medal of the Analytical Division of theRoyal Australian Chemical Instutute (2009), the Medal of the Japanese Association for Flow Injection Analysis(2010), and the Grimwade Prize in Industrial Chemistry from the University of Melbourne (2012).

He is the Founding Editor-in-Chief of the journal Membranes (MDPI) and member of the Editorial Boards of Analytica Chimica Acta (Elsevier), Talanta (Elsevier), Sensors (MDPI), Challenges (MDPI), EnvironmentalModeling and Assessment (Springer), and the International Journal of Analytical Chemistry (Hindawi).

Spas Kolev is a Fellow of the Royal Australian Chemical Institute.

http://www.chemistry.unimelb.edu.au/http://www.chemistry.unimelb.edu.au/http://www.chemistry.unimelb.edu.au/http://www.capim.com.au/http://www.capim.com.au/http://www.capim.com.au/http://www.capim.com.au/http://www.capim.com.au/http://www.chemistry.unimelb.edu.au/


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A/Prof Conor HoganCollege of Science, Health and EngineeringLaTrobe University

Dr Conor Hogan completed his PhD in Chemistry at Dublin City University in Ireland in2000 and following several years of postdoctoral research in Ireland (under Prof.Robert Forster) and Australia (under Prof. Alan Bond) was appointed as a lecturer in

the Department of Chemistry at La Trobe in 2003. Since 2009 he has been a senior lecturer in analyticalchemistry at the La Trobe Institute for Molecular Science where he leads one of the institute’s five researchthemes (Molecular Sensing). Research in his group is multidisciplinary. His fundamental research focuses onfocuses on the interface between electrochemistry and photochemistry and is driven by applications in the fieldof chemical sensors and biosensors. He is known internationally for his contributions to the field ofElectrochemiluminescence (ECL) and the development of mobile phone based sensing technologies. He is aFellow of the Royal Society of Chemistry and the Royal Australian Chemical Institute. He is active in particularwithin the electrochemical and analytical divisions of the RACI. He was chair of the Electrochemical Divisionof the RACI from 2011 to 2013 and he is currently Australia & New Zealand regional representative for the

International Society of Electrochemistry (ISE).

Dr Craig PriestFoundation Fellow (Senior Research Fellow)Future Industries InstituteUniversity of South Australia

I completed my PhD in 2005 at the University of South Australia (UniSA) on the physicalchemistry and interfacial science of wetting structured surfaces. I then completed a 2-year postdoc at the Max-Planck Institute for Dynamics and Self-Organization, before

returning to the Ian Wark Research Institute, UniSA, as a Research Associate. I am now a Foundation Fellow(Senior Research Fellow) at the newly-formed Future Industries Institute, UniSA. My research has led to 65publications in the fields of interfacial chemistry, thin films, wettability, micro- and nano-fluidics, nanomaterials,mineral extraction, analytical chemistry, and ionic liquid behavior. I was awarded the SA Early CareerResearcher of the Year Award in 2011 and joined the SA State Government’s Premier’s Science and IndustryCouncil for a three-year term (2012-2014).

Professor Weihua Li

Professor, School of Mechanical, Materials and Mechatronic EngineeringDirector, Advanced Manufacturing Technologies Research Strength

Weihua Li, PhD, is a Professor and Director of the Advanced Manufacturing ResearchStrength at the University of Wollongong. He obtained his B.Eng (1992) and M.Eng(1995) from the University of Science and Technology of China, and PhD fromNanyang Technological University, Singapore (2001). Since 2003, he has been

working as academic staff at the School of Mechanical, Materials and Mechatronic Engineering, University ofWollongong, Australia. His research focuses on smart materials and their applications, microfluidics, rheology,and intelligent mechatronics. He is serving as editor or editorial board member for more than 10 international

journals, including Scientific Reports, IEEE/ASME Transactions on Mechatronics, Smart Materials andStructures, RSV Advances, etc. He has published more than 260 journal and conference papers. He is arecipient of IOP Fellowship (2015), JSPS Invitation Fellowship (2014), Australian Endeavour Fellowship(2011), and Best Paper Awards.

http://www.unisa.edu.au/Research/Future-Industries-Institute/http://www.unisa.edu.au/Research/Future-Industries-Institute/


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9.00 am Dr Muhammad J. A. Shiddiky/ Prof Nam-Trung Nguyen, Griffith UniversityWelcome

Chair: Prof Nam-Trung Nguyen, Griffith University

9.10 am OPENING PLENARY

Prof Carolyn Ren, University of WaterlooDroplet Microfluidics – Enabling Technology for High Throughput Screening

9.50 am Prof Justin J. Cooper-White, University of QueenslandNext Generation Microdevices for Applications in Developmental Biology and Regenerative Medicine

10.20 am Prof Colin. L. Raston, Flinders UniversityThin Film Microfluidics

10.40 am MORNING TEA

Chair: Prof Justin J. Cooper-White, University of Queensland

11.00 am Prof Amanda V. Ellis, Flinders UniversitySurface Modifications in Microfluidic Devices

11.30 am Prof Yanyi Huang, Peking UniversityMicrofluidics Single Cell Sequencing

11.50 am Dr Simon R. Corrie, University of Queensland/Monash UniversityMicroprojection Arrays for Selective Capture of Dengue Proteins from the Skin

12.10 pm Dr Han Wei Hou, Nanyang Technological University An Integrated Point-of-Care Microdevice for Neutrophil Sorting and Chemotaxis Assay

12.25 pm Feng Li, University of TasmaniaNanoporous Membranes for Microfluidic Sample-in/Answer-Out Assay of Proteins in Urine

12.40 pm LUNCH

Chair: Prof Michael Breadmore, University of Tasmania

1.40 pm Prof Stephen J. Haswell, Deakin University What Can Micro Reactors Offer Chemical Synthesis and Bio Processing

2.10 am Dr Chun-Xia Zhao, University of QueenslandMicrofluidic Fabrication of Stable Double Emulsions for Controlled Release

2.30 am Muhsincan Sesen, Monash University Microfluidic Droplet Sensing, Splitting and Merging Using Capacitive Sensors and Surface AcousticWaves

2.45 pm Dr Huaying Chen, CSIRO ClaytonMultiplexed Biomarker Detection Using a Microfluidic Platform Integrating Single Bead Trapping and Acoustic Mixing

3.00 pm Dr Chau T. Lien, ANFF-Queensland Node (SPONSOR TALK # 1)Fabrication and Characterisation Capabilities at ANFF-Q

3.20 pm AFTERNOON TEA

Chair: Prof Amanda V. Ellis, Flinders University

3.40 pm Prof Spas D. Kolev, University of MelbourneEnvironmental Monitoring of Nutrients Using Paper-Based Microfluidic Sensors

4.10 pm Dr Ciprian Iliescu, Institute of Bioengineering and Nanotechnology, SingaporeMicrofluidic-Assisted Constrain Spheroids for Long Term Cell Culture

4.30 pm Dr Majid Ebrahimi Warkiani, University of New South Wales (UNSW) Advanced Microfluidic Systems for Cancer Research

4.50 pm Ziqiu (Tony) Tong, University of South AustraliaMicrofluidic Based Platform for High Throughput Screening of Nanoparticle Toxicity

5.05 pm CLOSE DAY 1

DAY 1, 21 March 2016, MONDAY


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1. Opening Plenary

DROPLET MICROFLUIDICS – ENABLING TECHNOLOGY FOR HIGH THROUGHPUT SCREENING

Carolyn Ren

Department of Mechanical and Mechatronics Engineering, University of Waterloo200 University Ave West, Waterloo, Ontario, Canada, N2L3G1Email: [email protected]

Droplet-based two-phase microfluidics enables high throughput screening analysis by utilizing monodispersednanoliter-sized droplets as mobilized test tubes. Other advantages of droplet microfluidics over traditional highthroughput technology include continuous flow offering continuous processing, minimized cross contaminationbenefiting from well encapsulated droplets, and rapid mixing due to three-dimensional flow occurring indroplets. Both gas-liquid and two immiscible liquids (water and oil) systems have been employed to makeliquid droplets in microfluidic platforms. This talk only focuses on the system employing two immiscible liquidsto generate droplets.

The first half of the talk will discuss fundamentals and physical modelling of droplet generation in T-junctions 1-3 and flow focusing geometries4-5 and droplet trafficking and sorting through a channel network6. The secondhalf will focus on electrical sensing and manipulation of droplets. In particular, capacitance sensing 7 andmicrowave sensing8-9 of droplets will be discussed and then followed with microwave heating and mixing of

droplets10

.References1. Glawdel, T.; Elbuken, C.; Ren, C.L. Phys Rev E , 2012, 85 , 016322 (9 pp). 2. Glawdel, T.; Elbuken, C.; Ren, C.L. Phys Rev E , 2012, 85 , 016323 (12 pp). 3. Glawdel, T.; Ren, C.L. Phys Rev E , 2012, 86 , 026308 (12 pages). 4. Chen, X.; Glawdel, T.; Cui, N.; Ren, C.L. Microfluidics Nanofluidics, 2015, 18 , 1341-1353. 5. Chen, X.; Ren, C.L., Phys Fluids, 2015, in rebuttal. 6. Glawdel, T.; Elbuken, C.; Ren, C.L. Lab Chip, 2011,11, 3774-3784 7. Elbuken, C.; Glawdel, T.; Chan, D.; Ren, C.L. Sens Actuator A: Phys, 2011, 171, 55-62.8. Boybay, M.S.; Jiao, A.; Glawdel, T.; Ren, C. L. Lab Chip, 2013, 13, 3840-3846. 9. Yesiloz, G.; Boybay, M.S.; Ren, C.L. Lab Chip, 2015, 21, 4008-4019. 10. Yesiloz, G.; Boybay, M.S.; Ren, C.L. Anal Chem, Submitted, 2016.

mailto:[email protected]:[email protected]


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2. Keynote

NEXT GENERATION MICRODEVICES FOR APPLICATIONS IN DEVELOPMENTAL BIOLOGY ANDREGENERATIVE MEDICINE

Justin J. Cooper-Whitea,b,c*

a Australian Institute for Bioengineering & Nanotechnology, The University of Queensland, St. Lucia, QLD4072, AUSTRALIA.bSchool of Chemical Engineering, The University of Queensland, St. Lucia, QLD 4072, AUSTRALIA.c Biomedical Manufacturing, Manufacturing Flagship, CSIRO, Clayton, Victoria 3169, AUSTRALIA.* [email protected] ; [email protected]

The successful deployment of human stem cells (pluripotent (hPSCs) or mesenchymal (hMSCs)) inregenerative medicine applications depends on effective control of both their undifferentiated expansion anddifferentiation into desired lineages. We have developed scalable, valveless, continuous-flow microdeviceplatforms to probe the impacts of a range of microenvironmental parameters on stem cell behaviours so as toeffect greater control over stem cell fate. For example, among these device platforms, our microbioreactorarrays (MBAs) have been designed to both provide a combinatorial set of defined factor compositions, andallow controlled accumulation of paracrine factors through the creation of perfused cellular microenvironments

in parallel. Through screens of pluripotency maintenance and differentiation of hPSCs into primitive streak,cardiac and kidney cells, we have demonstrated the unique ability of this platform to separate, visualise,identify and modulate paracrine effects that are not otherwise readily accessible with standard culture formats.Culture conditions optimized with the arrays are readily translated to conventional static culture protocols. Mostrecently we have assessed the impacts and interplay of developmental factors on proliferation of hPSC-derivedcardiomyocytes, exemplifying the potential utility of the device for patient-specific early drug stratification.These multiplexed microfluidic platforms can decipher factor interplay and signalling hierarchies that controlstem cell fate, and are applicable as microenvironmental screening platforms for developmental biology,bioprocess optimisation, media formulation design, quality control for cellular therapies and cell-based drugscreening.

mailto:[email protected]:[email protected]:[email protected]:[email protected]


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3. Invited THIN FILM MICROFLUIDICS

Colin. L. Raston*1

Centre for NanoScale Science and Technology, School of Chemical and Physical Sciences, FlindersUniversity, Bedford Park SA 5042, Australia* Corresponding Author: [email protected]

The presentation will highlight the application of a vortex fluidic device (VFD)1-4 which generates intense shearwithin dynamic thin films, allowing access to a diverse range of processing, from small molecule and materialssynthesis to protein folding and enhancing enzymatic reactions.

The ability to carry out chemical and biochemical processing under continuous flow is gaining prominence,where scalability is factored in at the inception of the fundamental science. To this end we have developed avortex fluidic device (VFD), Fig. 1,1 as a versatile thin film microfluidic platform. The VFD does not suffer fromclogging, unlike conventional channel based microfluidics, and the processing is not limited to diffusion control.Reaction rates and yields can be dramatically increased relative to conventional batch processing, as well asgaining access to new products and processing. This relates to the unique conditions imparted in the dynamicthin film in the device, including a vibronic response in the form of Faraday waves, high shear stress andmicromixing, and increased heat and mass and transfer.2 All molecules are treated in the same way in the thinfilm, which can be varied by varying the VFD control parameters, including concentrations, temperature, flowrates, tilt angle θ, rotational speed, and surface contact angle, as well as different Faraday waves and otherfield effects (magnetic, pulsed laser and UV, plasma), Fig.1. Within the thin films in the VFD, molecules andmaterials can be prepared and probed. Both bottom up and top down materials synthesis is possible, and canbe used to control the pore size and wall thickness of mesoporous materials, control the phase of materials,control the formation of graphene scrolls, slicing SWCNT and MWCNT < 400 nm, and exfoliate boron nitrideand graphene. Other applications abound in catalysis (including enhancing enzymatic reactions), probing thestructure of self-organised systems, reactivity and selectivity (assembly line synthesis),3 protein folding,4 andmore

References1. Yasmin, L.; Chen, X.; Stubbs, K. A.; Raston, C. L. Scientific Reports, 2013, 3, 2282.2. Britton, J.; Dalziel, S. B.; Raston, C. L. RSC Advances 2015, 5 , 1655.2.3. Britton, J.; Chalker, J.; Raston, C. L. Chem. Eur. J., 2015, 21, 10660.4. Yuan, T. Z.et al., ChemBioChem, 2015, 16 , 393.

Figure 1. The vortex fluidic device (VFD) and some applications in materials, chemical andbiochemical processing.



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4. Keynote SURFACE MODIFICATIONS IN MICROFLUIDIC DEVICES

Amanda V. Ellis*1

1Flinders Centre for Nanoscale Science and Technology, Flinders University, School of Chemical andPhysical Sciences, Sturt Road, Bedford Park, Adelaide, SA 5042, Australia* Corresponding Author: [email protected]

This talk discusses various strategies to create novel surfaces inside microfluidic channels. In particular,modification with oligonucleotides, carbon nanotubes and gold nanoparticles. Work will be presented on thedevelopment of a new primer system (using hairpin-looped primers) that allows for the hybridisation of double-stranded PCR products on capture probes immobilised onto a surface of poly(dimethylsiloxane) (PDMS)microfluidic devices.1,2 The development of a PDMS microfluidic reactor with an interface layer made of carbonnanomaterials (multi- and single-walled CNTs,). The CNT layer provides an “electrical connection” betweentwo physically separated microfluidic channels allowing for contactless open circuit RedOx reactions to beperformed between reagents passing through two the microchannels. Finally, a discussion of off-stoichiometricthiol-ene (OSTE) polymers in combination with a one-step UV lamination “click” reaction that afford theopportunity to rapidly create microchannels with low volume shrinkage and optical transparency will bepresented. A further benefit is pendant alkene groups can be utilised to attach nanoparticles, in this case gold

nanoparticles to form gold films as potential electrodes inside microchannels.

References1. Khodakov, D. A., Thredgold, L. D., Lenehan, C. E., Andersson, G., Kobus, H. J., Ellis, A. V., Biomicrofluidics

2012, 6 (2), 026503-1-026503-11.2. Khodakov, D. A., Khodakova, A., Linacre, A., Ellis, A. V. J. Am. Chem. Soc. 2013, 135 (15), 5612-5619.

5. Invited MICROFLUIDICS SINGLE CELL SEQUENCING

Yanyi Huang

1Biodynamic Optical Imaging Center (BIOPIC), School of Life Sciences, College of Engineering, Peking-Tsinghua Center for Life Sciences, Peking University, Beijing 100871, China.* E-mail: [email protected]

Quantitative single-cell analysis enables the characterization of cellular systems with a level of detail thatcannot be achieved with ensemble measurement. I am going to show some of our recent work on developingbetter approaches for single-cell sequencing. Whole-genome amplification (WGA) for next-generationsequencing has seen wide applications in biology and medicine when characterization of the genome of asingle cell is required. High uniformity and fidelity of WGA is needed to accurately determine genomicvariations, such as copy number variations (CNVs) and single- nucleotide variations (SNVs). Prevailing WGAmethods have been limited by fluctuation of the amplification yield along the genome, as well as false-positiveand -negative errors for SNV identification. Here, we report emulsion WGA (eWGA) to overcome theseproblems. We divide single-cell genomic DNA into a large number (105) of picoliter aqueous droplets in oil.Containing only a few DNA fragments, each droplet is led to reach saturation of DNA amplification beforedemulsification such that the differences in amplification gain among the fragments are minimized. Wedemonstrate the proof-of-principle of eWGA with multiple dis- placement amplification (MDA), a popular WGAmethod. This easy-to-operate approach enables simultaneous detection of CNVs and SNVs in an individualhuman cell, exhibiting significantly improved amplification evenness and accuracy.

References1. Yusi Fu, Chunmei Li, Sijia Lu, Wenxiong Zhou, FuchouTang, X.Sunney Xie,* Yanyi Huang, PNAS 2015,

112(38), 11923-11928.



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6. Invited MICROPROJECTION ARRAYS FOR SELECTIVE CAPTURE OF DENGUE PROTEINS FROM THE SKIN

Simon R. Corrie* and Mark A. F. Kendall

Australian Institute for Bioengineering and Nanotechnology, ARC Centre of Excellence in Convergent Bio-Nano Science and Technology, University of Queensland, St Lucia, QLD, 4072, Australia* Corresponding Author: [email protected]

While novel capture and detection aspects of immunosensor technology continue to attract significant researchinterest, progress is hampered by the reliance on need/syringe-based sampling of unpurified body fluidsamples. The protein content of blood is dominated by several high-concentration species1 such that complexsample processing methods are often carried out in a laboratory prior to detection of the target protein. In thecontext of infectious disease diagnostics, particularly in remote areas, access to laboratory infrastructure andexpert clinicians/technologists is severely restricted.

In addressing this challenge, we have developed a device for biomarker-selective capture of proteins andantibodies directly from the skin, using “Microprojection arrays” coated with anti-fouling polymers andbiomarker-selective probes2. A microfabrication approach (DRIE) was used to produce silicon “master” arrays,from which polycarbonate arrays were then copied using PDMS negative moulds in a hot embossing process3.Our most recent findings describe the basic mechanism underlying the process of biomarker capture from the

skin in situ, and furthermore demonstrate application of Microprojection arrays for the detection of dengue NS1protein in murine models of dengue fever.

References1. Anderson, N. L.; Anderson, N. G.; Mol. Cell. Proteomics. 2002, 1, 845-867. 2. Corrie, S. R.; Fernando, G. J. P.; Crichton, M. L.; Brunck M. E. G.; Anderson, C. D.; Kendall, M. A. F.; Lab

Chip. 2010, 10 , 2655-2658. 3. Yeow, B.; Coffey, J. W.; Muller, D. A.; Grondahl, L.; Kendall, M. A. F.; Anal. Chem. 2013, 85 , 10196-10204.



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7.AN INTEGRATED POINT-OF-CARE MICRODEVICE FOR NEUTROPHIL SORTING AND CHEMOTAXISASSAY

Hui Min Tay1, Chayakorn Petchakup2, Bernhard O. Boehm1,3, King Ho Holden Li2 and Han Wei Hou*1

1 Lee Kong Chian School of Medicine, Nanyang Technological University, 59 Nanyang Drive, Singapore636921, Singapore. 2 Mechanical and Aerospace Engineering, Nanyang Technological University, 50

Nanyang Avenue, Singapore 639798, Singapore. 3Endocrine and Diabetes, Tan Tock Seng Hospital, 11Jalan Tan Tock Seng, Singapore 308433, Singapore.* Corresponding Author: [email protected]

Diabetes mellitus is a metabolic disorder characterized by chronic hyperglycaemia resulting in increasedoxidative stress, inflammation and endothelial dysfunction1. While clinical studies have reported abnormalneutrophil chemotaxis in diabetic patients2, 3, the study of leukocyte dysfunctions remains technicallychallenging due to laborious leukocyte isolation methods (density gradient centrifugation and blood lysis). Inthis work, we introduce a novel integrated microdevice for single-step neutrophil sorting and chemotaxis assayfrom whole blood directly. Using small blood volumes (~10 µL), neutrophils are enriched using cell margination4 and the purified neutrophils are subsequently exposed to a diffusion based chemotactic gradient to initiatecellular migration. The unique strategy of integrating neutrophil sorting with chemotaxis assay for point-of-caretesting offers several key advantages including rapid, single step neutrophil enrichment and purification (~10minutes using a drop of blood) and well controlled microenvironment to generate linear and stable chemotactic

gradients. Device operation using syringe pump is easy and the user only has to load blood sample into thedevice. We envision that characterization of neutrophil chemotaxis in diabetes patients can provide directevidence in microvascular compilations in diabetes, and be used as surrogate biomarkers for monitoringendothelial dysfunction, arterial stiffness, peripheral markers of inflammation and oxidative stress in metabolicdiseases.

References1. Hartge, M. M.; Unger, T.; Kintscher, U., Diabetes and Vascular Disease Research 2007, 4 (2), 84-88.2. Mowat, A. G.; Baum, J., New England Journal of Medicine 1971, 284 (12), 621-627.3. Delamaire, M.; Maugendre, D.; Moreno, M.; Le Goff, M. C.; Allannic, H.; Genetet, B., Diabetic Medicine

1997, 14 (1), 29-34.4. Hou, H. W.; Bhagat, A. A. S.; Chong, A. G. L.; Mao, P.; Tan, K. S. W.; Han, J.; Lim, C. T., Lab on a Chip

2010, 10 (19), 2605-2613.

8. NANOPOROUS MEMBRANES FOR MICROFLUIDIC SAMPLE-IN/ANSWER-OUT ASSAY OF PROTEINSIN URINE

Feng Li1,2, Rosanne M Guijt2, Michael C Breadmore1

1 Australian Centre for Research on Separation Science, School of Chemistry, University of Tasmania,

Private Bag 75, Hobart, Tasmania 7001, Australia.2 Australian Centre for Research on Separation Science, School of Pharmacy, University of Tasmania,Private Bag 26, Hobart, Tasmania 7001, Australia. Email: [email protected]

Microfluidic device integrating nonporous membrane based sample preparation with electrophoretic separationwas developed. This device was fabricated by sandwiching two different nanoporous polycarbonate tracketched (PCTE) membranes between two PDMS slabs embedded with microchannels. It has beendemonstrated our device integrated the protein extraction, purification, concentration and separation into asingle chip based on size selectivity of these two membranes. The first larger membrane was a filter andextractor, it can be used to selectively extract and inject proteins with appropriate size while preventing largerinterferences from sample matrix. The second smaller membrane was a concentrator which canpreconcentrating target proteins, at the same time it can also purify the proteins by removing the smallinterferences ions and smaller proteins. By using this device, sample-in/answer-out analysis of albumin in

human urine has been achieved within 1 min, and the linear range of 0-100 μg mL-1 of albumin covers thediagnostic level of albuminuria of 30μg mL-1, which shows our device has a great potential in point-of-careprotein analysis from body fluids.



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9. Keynote WHAT CAN MICRO REACTORS OFFER CHEMICAL SYNTHESIS AND BIO PROCESSING

Stephen J. Haswell

Deakin University, Geelong, VIC 3220, Australia, Centre for Regional and Rural Futures.Email: [email protected]

In this presentation the fundamental aspects of micro reactor systems together with their operationalproperties will be described. The systems used have been constructed from borosilicate glass to give a

network of etched channels in the range 50-300 m. Hydrodynamic pumping and applied electric fieldsto create electroosmotic flow and electrokinetic mobilisation within the capillaries are used to transportand separate the nl volumes of reagents and reaction products. Examples will be given of a number ofreactions that have now been carried out in micro reactors to illustrate the practical advantages of thetechnology. Having described and illustrated the advantages micro reactor technology can bring interms of micro fluidic manipulations, the presentation will next consider how reaction intensificationconditions and monitoring can be exploited in an integrated way. In terms of reaction intensificationexamples will be given to show how modified surfaces and localised heating can be used to achievegreater flexibility and control of chemical and biological processing.

Currently the analytical finish carried out to characterise the chemistry performed with micro reactors

has relied mainly on off chip GC MS, HPLC and NMR measurements, however the in situ use of currentand pressure monitoring has proved to be an attractive inferential approach to reaction monitoring, fromwhich dynamic reaction information can be obtained. Examples will be given of how inferential basedmeasurements could provide a beneficial measurement strategy in micro reactor devices, where a highlevel of chemical control is achievable. Finally the relevance of system integration will be outlined asone of the major strengths of adopting a micro reactor approach to chemical and biological processing.

10. Invited

MICROFLUIDIC FABRICATION OF STABLE DOUBLE EMULSIONS FOR CONTROLLED RELEASE

Chun-Xia Zhao,*1,2 Dong Chen,2 David A. Weitz1 and Anton P. J. Middelberg1

1 Australian Institute for Bioengineering and Nanotechnology, The University of Queensland, St Lucia QLD4072, Australia. 2 School of Engineering and Applied Sciences and Department of Physics, HarvardUniversity, Cambridge, Massachusetts 02138, United States. * Corresponding Author: [email protected]

Double emulsions have attracted significant interest because of their hierarchical structure of dropletsencapsulated in droplets, and have been applied for various applications, such as food, pharmaceuticals, etc[1-3]. Traditional methods of making double emulsions involve a two-step process, firstly forming the primaryemulsions then followed by further emulsifying the primary emulsions in an external phase. These approacheslack control over the structure and size of the resulting double emulsions. Microfluidic technology offers a facile

way to make complex emulsions with various structures (double, triple and even higher hierarchical emulsions)and controlled properties. However, the stability of double emulsions, which is essential for practicalapplications, remains a big challenge due to their inherent thermodynamic instability. To improve the stabilityof double emulsions, we developed a microcapillary method for making ultra-thin shell double emulsions, whichcan be stable up to months. Different parameters were investigated to control the formation of ultra-thin-shelldouble emulsions, and optimize the system and operation conditions that are facile for the formation of ultra-thin-shell double emulsions. Different sizes of monodisperse double emulsions formed in the dripping regimeshowed good stability over a long period of time and small molecules can be retained in the inner dropletwithout a trigger. In contrast, rapid release can be achieved by osmolarity shock. This work demonstrated thesignificant potential of stable double emulsions in controlled release.

References1. L.-Y. Chu, A.S. Utada, R.K. Shah, J.-W. Kim, D.A. Weitz, Angewandte Chemie-International Edition, 46

(2007) 8970-8974.2. A.T. Florence, D. Whitehill, International Journal of Pharmaceutics, 11 (1982) 277-308.3. S. Matsumoto, Y. Kita, D. Yonezawa, Journal of Colloid and Interface Science, 57 (1976) 353-361.



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11.

MICROFLUIDIC DROPLET SENSING, SPLITTING AND MERGING USING CAPACITIVE SENSORS ANDSURFACE ACOUSTIC WAVES

Muhsincan Sesena, Tuncay Alana, and Adrian Neild*a

a Lab for Microsystems, Monash University, Clayton, VIC, Australia.

* Corresponding Author: [email protected]

Exhaustive analytical studies can be carried out in rapid succession in microfluidic devices when aqueousdroplets are dispersed in a carrier fluid. Droplet microfluidics offers chemical and physical isolation of dropletswhere every single droplet can be identified as a mini reaction compartment. Mono-disperse droplet formationand further manipulation capabilities allows a multitude of studies to be carried out with these systems. In thiswork, the droplets are first sensed by a capacitive sensor so that they can be split unevenly on-demand at aT-junction by-pass using surface acoustic waves (SAWs). SAWs couple into a fluid medium with Rayleighangle and this leads to acoustic streaming that can drive the carrier fluid in the by-pass channel. The by-passchannel is designed to have high resistance so without SAW actuation, the droplets do not split, however,when SAW is applied, acoustic streaming in the by-pass channel creates a suction effect in the main channelthereby splitting a droplet at the junction into two. A number of parameters determine the final split daughterdroplet volume and these parameters are thoroughly characterised by experiments and simulation.

Furthermore, the split droplets could be trapped in a hydrodynamic merging chamber and merged with thesecond split droplet so that a combinatorial library is formed. The proposed system can easily be integrated toexisting lab-on-a-chip devices and it offers a robust and contamination-free droplet manipulation technique inclosed microchannels that can be used with screening studies in an attempt to find the desired chemicalreaction.

12.MULTIPLEXED BIOMARKER DETECTION USING A MICROFLUIDIC PLATFORM INTEGRATINGSINGLE BEAD TRAPPING AND ACOUSTIC MIXING

Huaying Chen1, Yuan Gao1, Karolina Petkovic-Duran1, Michael Best1, Vicky Boyd2 and Yonggang Zhu*1

1 Microfluidics and Fluid Dynamics Team, CSIRO Manufacturing and Biosecurity Flagship, Private Bag 10,Clayton, VIC, 3169, Australia.2 Biosecurity Flagship, CSIRO, Private Bag 24, Geelong, VIC, 3220, Australia.*Corresponding Author: [email protected]

Rapid detection of multiple biomarkers in a small amount of sample is highly demanded in the pathogen/virusdetection in clinics, food safety control, environment monitoring and homeland security, etc. In this paper, wereport the development of a microfluidic platform for the rapid, simultaneous detection of multiple biomarkersusing immunoassay. The microfluidic platform is based on simple single magnetic beads trapping techniqueand bubble-induced acoustic micromixing. It consists of i) a PDMS microfluidic chip containing air bubble traps

and a glass slide coated with permalloy microarray, ii) a vacuum pump and solenoid valves to handle the liquidreagents, and iii) a piezo transducer to generate ultrasound to mix nanoliter samples. This device enables a)trap and release of single magnetic microbeads, b) rapid and active mixing of low concentration analytes andbeads conjugated with antibodies and c) imaging of individual beads for multiplexed detection. A proof-of-concept study has shown that the platform can simultaneously detect both PSA and CEA with a limit ofdetection of around 1 ng/mL. The novelties of the device are a) active bubble-induced micromixing, whichdramatically reduces the assay time from hours to less than 20 min; and b) robust and simple single beadtrapping, which not only reduces the assay time by fast sample loading and washing, but also enables singlebead identity (a critical requirement for multiplexed detection. This platform has a great potential for multiplexedbiomarker detection with high sensitivity using color-coded beads and other imaging techniques such assurface enhanced Raman spectroscopy.



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13. Sponsor Talk # 1 FABRICATION AND CHARACTERISATION CAPABILITIES AT ANFF-Q

Chau T. Lien

The Queensland Node of the Australian National Fabrication Facility (ANFF-Q), AIBN, The University ofQueensland, St. Lucia QLD 4072, Australia. Email: [email protected]

Learn how the Queensland Node of the Australian National Fabrication Facility (ANFF-Q) can assist yourresearch. ANFF-Q provides open access to state-of-the-art fabrication capabilities to national and internationalacademic researchers and industry personnel. ANFF-Q has sites at the Australian Institute for Bioengineeringand Nanotechnology (AIBN) and the Centre for Organic Photonics and Electronics (COPE) at The Universityof Queensland and the Queensland Micro- and Nanotechnology Centre (QMNC) at Griffith University and ispart of a national network of 8 nodes and 19 institutions across Australia. With cutting-edge equipment andhighly experienced staff, ANFF-Q can assist you with the development of ideas, creating prototypes andcharacterising outcomes in a wide range of areas including microfluidics and MEMS, micro and nanoelectronics, sensors and medical devices, biomaterials, silicon carbide on silicon deposition, and novelsubstrates. Projects assisted by ANFF-Q include the development of nanopatch microprojection“nanoneedles” to replace traditional injections, the development of a device to capture and detect specificcancer cells in blood, the study of stimuli-responsive peptides and their applications in pharmaceuticals tofunctional foods, the improvement of mine safety through the application of novel materials, the investigation

of novel biocidal agents to improve water treatment systems, and the development of next generation organicsolar cells.



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14. Keynote ENVIRONMENTAL MONITORING OF NUTRIENTS USING PAPER-BASED MICROFLUIDIC SENSORS

Spas D. Kolev*

School of Chemistry, The University of Melbourne, VIC 3010, Australia* Corresponding Author: [email protected]

Paper-based microfluidic sensors have gained considerable popularity in recent years as a new type ofdisposable analytical sensing devices which meet the increasing needs of rapid, accurate and low-costmonitoring and analysis for environmental protection and healthcare. They utilize the capabilities of cellulosefibres in paper, which form a hydrophilic porous matrix, to transport liquids by capillary force only.

The present paper describes the development and application of paper-based microfluidic sensors forenvironmental monitoring of nutrients such as phosphate, nitrite, nitrate, and ammonia.1-4 The hydrophilic liquidpenetration channels and detection zones in these sensors were ink-jet printed using a paper-sizing agent.Colour analytical reactions were utilized for analyte detection with the colour intensity being measured by aconventional flatbed scanner. Complex on-line sample pre-treatment steps such as reduction of nitrate to nitriteand membrane-based gas-diffusion separation of ammonia were successfully implemented for the first time inthe proposed paper-based sensors thus further expanding their analytical capabilities. The paper-based

sensors, mentioned above, were applied to natural samples and very good agreement with the correspondingreference methods was observed.

References1. Jayawardane, B. M.; McKelvie, I. D.; Kolev, S. D. Talanta 2012, 100 , 454-460. 2. Jayawardane, B. M.; Wongwilai, W.; Grudpan, K.; Kolev, S. D.; Heaven, M. W.; Nash, D. M.; McKelvie, I.

D. J. Env. Qual. 2014, 43, 1081-1085. 3. Jayawardane, B. M.; Shen, W.; McKelvie, I. D.; Kolev, S. D. Anal. Chem. 2014, 86 , 7274-7279. 4. Jayawardane, B. M.; McKelvie, I. D.; Kolev, S. D. Anal. Chem. 2015, 87 , 4621-4626.

15. Invited MICROFLUIDIC-ASSITED CONSTRAIN SPHEROIDS FOR LONG TERM CELL CULTURE

Ciprian Iliescu,1 * Fang Yu,1,2 Hanry Yu1,2

1Institute of Bioengineering and Nanotechnology, 31 Biopolis Way, The Nanos, Singapore.2 National University of Singapore.* Corresponding Author: [email protected]

Numerous microfludic cell culture platforms have been developed with a monolayer of cells growing on 2Dsubstrates. The main drawback for 2D culture system is that it lacks the cell-cell interaction as presented invivo. In vivo, cells are present in many layers where they interact with each other by providing biological,chemical and mechanical cues. Therefore, 3D cell cultures system where multiple layers of cells are interactingwith each other are able to recapture in vivo cell morphology, gene expression profile and biological activitiesbetter than cells cultured in 2D system.

The present work underlines the main achievements of our team in the field: a 3D culture model namedConstrain Spheroids (CS) that can operate in both static or perfusion culture. In this model the cells aggregatesin spheroids which are stabilized in a sandwich configuration between surface modified glass slide and amicrofabricated ultra-thin Parylene C membrane. This allows us to maximize mass transfer, and overcomeuneven cell count and spheroids size issues. The glass substrate was modified for more uniform and rapidhepatocytes spheroids formation within 1 day, allowing for earlier drug testing and perfusion culture initiation.The top substrate is an ultra-thin, inert and flexible parylene membrane fabricated using MEMS technologies,special design to entrap the spheroids. We found that the perfusion constrain-spheroids culture maintain abetter polarity.



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9.00 am Dr Muhammad J. A. Shiddiky, Griffith University Announcement

Chair: Dr Geoff R. Willmott, The University of Auckland

9.10 am Prof Justin Gooding, University of New South Wales (UNSW) Towards Single Molecule Sensors

9.40 am Prof Alberto Redaelli, Politecnico di Milano A Novel Lab-on-a-Chip Microfluidic Platform to Monitor The Shear-Induced Thrombotic Risk

10.00 pm Dr Ramanathan Vaidyanathan, University of QueenslandNext-Gen Immunoassays: Enhancing Target Capture Using Tunable Surface Shear Forces andNovel Affinity Reagents

10.20 pm Ellen Otte, University of Queensland/ CSIRO ClaytonUsing a Microfluidic Device to Isolate and Investigate Different Modes of Cell-Cell Communication


Chair: Prof Justin Gooding, University of New South Wales (UNSW)

11.00 am Dr Yonggang Zhu, CSIRO ClaytonMicrofluidics for Biodetection and Energy

11.30 am Dr Niall Macdonald, University of Tasmania3D Printing Microfluidic Devices – Which Printer Type?

11.50 am Chin Hong Ooi, Griffith UniversitySelf-Propelled Motion and Evaporation of a Floating Liquid Marble

12.05 pm Frederik H. Kriel, University of South Australia Extraction and ‘Scale-Up’ of Platinum Using Microfluidic Solvent Extraction

12.20 pm Kara B. Spilstead, Deakin UniversityExploring Coloured Materials for Application in Chemiluminescence Detection Flow Cells andMicrofluidic Chips

12.35 pm LUNCH

Chair: Prof Stephen J. Haswell, Deakin University

1.35 pm Dr Geoff R. Willmott, The University of AucklandTrajectories and Charge in Tunable Resistive Pulse Sensing

2.05 am Dr Egan H. Doeven, Deakin UniversityDevelopment of a Lab on a Chip System for The Detection of Influenza Virus

2.25 am Dan Yuan, University of Wollongong Selective Lateral Transport of Particles and Solution Exchange in Viscoelastic Fluid

2.40 pm Dr Craig Priest, University of South Australia (SPONSOR Talk # 2) ANFF – South Australia: Chips and Interfaces for Micro/Nanofluidics

3.00 pm Mr Alan Iacopi, QLD Micro and Nanotechnology Centre (SPONSOR Talk # 3)The Queensland Microtechnology Facility – Innovation Centre and Capabilities

3.20 pm AFTERNOON TEA

Chair: Dr Rosanne M Guijt, University of Tasmania

3.50 pm Dr Conor F. Hogan, La Trobe University Android Voltammetry: Low Cost Electrochemical Detection for Paper Microfluidic Sensors Using aMobile Device

4.20 pm POSTER SESSION

5.20 pm CLOSE DAY 2

6.30 pm CONFERENCE DINNER (Stone Restaurants & Bar, 161 Grey Street, South Bank, Brisbane)

DAY 2, 22 March 2016, TUESDAY


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18. Keynote TOWARDS SINGLE MOLECULE SENSORS

Yuanhui Zheng1, Xun Lu1, Alexander H. Soeriyadi1, Xiaoyu Cheng1,2, Thibault Tabarin2, Phillip Nicovich2,Lorenzo Rosa3,4, Soon Hock Ng5,6, Udo Bach5,6, Katharina Gaus2 and J. Justin Gooding1*

1 School of Chemistry, Australian Centre for NanoMedicine and the ARC Centre of Excellence in ConvergentBio-Nano Science and Technology, University of New South Wales, Sydney, 2052, Australia

2 EMBL Australia Node in Single Molecule Science, School of Medical Sciences and the ARC Centre ofExcellence in Advanced Molecular Imaging, University of New South Wales, Sydney, 2052, Australia3Swinburne University of Technology, Centre for Micro-Photonics (H34), P. O. Box 218, Hawthorn, Victoria3122, Australia.4Department of Information Engineering, University of Parma, V.le G.P. Usberti, 181/A I-43124 Parma, Italy5 Department of Materials Engineering, Monash University, Wellington Road, Clayton, Victoria 3800,

Australia.6 The Melbourne Centre for Nanofabrication, 151 Wellington Road, Clayton, Victoria 3168.

*Corresponding author: Email [email protected]

Single molecule sensors are in some ways the ultimate analytical device. We are interested in developing

single molecule sensors for quantitative analysis. Conventional single molecule measurements are bound byupper and lower concentration limits. A consideration of these concentration limits provides clues on how todevelop quantitative single molecule sensors [1]. A number of strategies are being tried which we haveclassified as i) near field-massively parallel, ii) wide field sampling-near field detection and iii) wide fieldmeasurements [2]. This talk will outline work done in our laboratory that addresses these different strategies.The first is a single molecule surface enhanced Raman spectroscopy as a wide field sampling-near fielddetection approach [3] and the second employs single molecule localisation microscopy as a wide fieldmeasurement method [4].

References1. P. Holzmeister, P.; Acuna, G. P.; Grohmann, D.; Tinnefeld, P. Chem. Soc. Rev . 2014, 43, 1014-1028. 2. Gooding, J. J.; Gaus, K. Angew. Chem. Int. Ed. Submitted 3. Y. Zheng, A. H. Soeriyadi, L. Rosa, S. H. Ng, U. Bach, J. J. Gooding, Nature Comm. 2015, 6 , 8797.

4. X. Lu, T. Tabarin, P. Nicovich, S. R. C. Vivekchand, K. Gaus, J. J. Gooding, unpublished.



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19. Invited A NOVEL LAB-ON-A-CHIP MICROFLUIDIC PLATFORM TO MONITOR THE SHEAR-INDUCEDTHROMBOTIC RISK

Annalisa Dimasi1, Filippo Consolo1, Marco Rasponi1, Gianfranco B. Fiore1, Lorenzo Valerio1,2, FedericoPappalardo2, Danny Bluestein3, Marvin J. Slepian4, Alberto Redaelli*1

1Department of Electronics, Information and Bioengineering, Politecnico di Milano, Piazza Leonardo da Vinci

32, 20133, Milano IT2 Department of Anesthesia and Intensive Care, IRCCS San Raffaele Scientific Institute, Via Olgettina 5820132. Milano IT3Department of Biomedical Engineering, Stony Brook University, Stony Brook, New York 11794, USA4Department of Medicine and Biomedical Engineering, University of Arizona, 1501 N Campbell Ave, Tucson,

AZ , USA


Shear-induced thrombosis due to platelet activation (PA) can cause device failure and serious post-implantcomplications for the recipients of blood contacting devices (BCDs) such as ventricular assist devices (VADs).However, standard in vitro flow-based assays require large volumes of sample and reagents and do not allowhigh-throughput experiments. Furthermore, "hyper-shear" conditions and fast dynamics of shear stresspatterns typically occurring in BCDs cannot be replicated in viscometer-based devices. To overcome theselimits we used PDMS-based microfluidic technology to replicate realistic flow patterns in BCDs under highlycontrolled conditions [1]. As a first step we simulated the behavior of the Heart Assist V (MicroMed TechnologyInc., USA) VAD. In vitro tests of PA were performed by using the platelet activity state (PAS) assay [2]. Bloodwas withdrawn from healthy adult volunteers, filtered and subsequent gel-column filtration of platelet richplasma. Experiments were conducted by flowing the gel-filtered plasma (GFP) sample through the microfluidicplatforms by means of two synchronized syringe pumps working in a reciprocating mode so that the sampleflew alternatively in two directions through the device.

An increasing trend of activation was observed between 0 and 40 passages, while a quasi-plateau behaviourwas observed from 40 to 72 passages, suggesting that a limit of platelet activation was reached at longerexposure time. The study represents a first application of microfluidic platforms to perform shear-induced testsof PA under dynamic and VAD-like shear flow conditions. In perspective it can be used to test antiplateletdrugs and assess/compare different devices.

References1. Dimasi, A.; Rasponi, M.; Sheriff, J.; Chiu, W.C.; Bluestein, D.; Tran, P.L.; Slepian, M.J.; Redaelli, A. Biomed.

Microdevices, 2015, 17 , 117. 2. Bluestein, D.; Girdhar, G.; Einav, S; Slepian, M.J. J. Biomech. 2013, 46 , 338-344.


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20. Invited NEXT-GEN IMMUNOASSAYS: ENHANCING TARGET CAPTURE USING TUNABLE SURFACESHEAR FORCES AND NOVEL AFFINITY REAGENTS

Ramanathan Vaidyanathan,1 Yadveer S. Grewal,1 Lauren J. Spadafora,3 Muhammad J.A. Shiddiky,1,,*Gerard A. Cangelosi3 and Matt Trau1,2*

1Centre for Personalised NanoMedicine, Australian Institute for Bioengineering and Nanotechnology (AIBN),

Corner College and Cooper Roads (Bldg 75), The University of Queensland, Brisbane QLD 4072, Australia2 School of Chemistry and Molecular Biosciences, The University of Queensland, Brisbane, Queensland4072, Australia3School of Public Health, University of Washington, Seattle, WA, USA

Email: [email protected]; [email protected]*; [email protected]*

New high-performance detection technologies and more robust protein capture agents can be combined toboth rapidly and specifically capture and detect protein biomarkers associated with disease in complexbiological samples. Recently, we have developed a simple methodology using tunable alternating current (ac)electrohydrodynamics (ac-EHD) forces generated within few nanometers of an electrode surface ( i.e., doublelayer), referred to as nanoshearing . Over the past two years, we have extensively explored this phenomenonfor the removal of nonspecifically adsorbed species from the sensor surface and also for the highly specificcapture of cellular species,1 molecular analytes2-4 as well as the manipulation of colloidal particles.5 Thispresentation shall review some of these recent developments, highlighting the applicability of this approach forsurface-enhanced Raman scattering (SERS) immunoassays and rapid detection (< 5 min) of proteinbiomarkers in combination with recently developed stable recombinant affinity reagents, namely nanoyeast-scFv.

References

1. Vaidyanathan, R.; Shiddiky, M. J. A.; Rauf, S.; Dray, E.; Tay, Z.; Trau, M. Anal. Chem. 2014, 86 , 2042-49.2. Shiddiky, M. J. A.; Vaidyanathan, R.; Rauf, S.; Tay, Z.; Trau, M. Sci. Rep. 2014, 4, 3716.3. Wang, Y.; Vaidyanathan, R.; Shiddiky, M. J. A.; Trau, M. ACS Nano 2015, 9, 6354-6362.

4. Vaidyanathan, R.; Rauf, S.; Grewal, Y. S.; Spadafora, L. J.; Shiddiky, M. J. A.; Cangelosi, G. A.; Trau, M. Anal. Chem. 2015, 87 , 11673-11681.5. Rauf. S.; Shiddiky, M. J. A.; Trau, M.; Chem. Commun. 2014, 50 , 4813-15.


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21.USING A MICROFLUIDIC DEVICE TO ISOLATE AND INVESTIGATE DIFFERENT MODES OF CELL-CELL COMMUNICATION

Ellen Otte1,2, Guannan Su2, Nick Glass1 and Justin Cooper-White1,2,3,*

1 Australian Institute of Bioengineering and Nanotechnology, University of Queensland, Cnr College Rd andCooper Rd, St Lucia, QLD, 4072

2 Manufacturing, CSIRO, Normanby Rd, Clayton, VIC, 31983School of Chemical Engineering, University of Queensland, St Lucia, QLD, 4072* Corresponding Author: [email protected]

Efficient and complete differentiation of stem cells is an important aspect of successful tissue engineering.Differentiation, like all cellular processes, is regulated by a complex combination of biological and abioticfactors. During development in vivo communication between cell populations is essential for cell specification.The ability of cells to release soluble factors to affect the cells around them is regularly exploited, as the additionof soluble factors is the basis of most differentiation protocols. Missing is the contribution of direct contact withother cell populations, as this has so far been difficult to isolate and investigate. In order to isolate these cellcommunication modes, a microfluidic device has been designed allowing direct contact and paracrinesignalling to be spatially separated, whilst their effects on cells are quantifiable with the use of fluorescentreadouts in situ. This has been applied to investigate the effect of cell-cell communication on mesenchymal

stem cells when exposed to a number of different cell types. The ability to grow cell populations that havedirect contact with each other but do not share a fluid/media environment has allowed insight into thecommunication between endothelial cells and MSCs, which are difficult to co-culture due to incompatible mediarequirement. These experiments have shown that contact with endothelial cells decreases the osteogenicdifferentiation of MSCs, with important implications for the generation of vascularized bone tissue constructs.

22. Keynote MICROFLUIDICS FOR BIODETECTION AND ENERGY

Huaying Chena, Yanping Dua, Karolina Petkovic-Durana, Yuan Gaoa, Yinan Zhangb, Clifford Shuma,c, NamLea, G. Bradburya, Guy Metcalfea, Min Gud and Yonggang Zhua*

aCSIRO Manufacturing Flagship, Private Bay 10, Clayton, VIC 3169, Australia.bSwinburne University of Technology, Hawthorn, Victoria 3122, Australia.c RMIT University, SAMME, Building 57, Carlton VIC 3053, Australiad RMIT University, GPO Box 2476, Melbourne, VIC 3001, Australia*[email protected]

The field of microfluidics has grown significantly since the past two decades due to the wide variety ofmultidisciplinary such as sensing, drug development, energy and so on. This presentation will cover some ofthe latest research and development activities in CSIRO Microfluidics Laboratories. It will mainly focus on the

investigations of capillary and chaotic flows in microgeometries and the associated applications in thedevelopment of microchip based point of care diagnostic device for virus detection and a high efficiency heattransfer device for solar cells.

To address the main challenges for point-of-care devices such as speed, reliability and portability, micromixingtechniques have been developed to speed up the biochemical assay process. Both acoustic and magnetictechniques are used to achieve chaotic mixing at microscales. The assay time can be significantly reducedfrom hours down to minutes. Detections of Hendra virus antibody from infected horse blood samples andseveral cancer biomarkers have been demonstrated using the developed microfluidic device. For the energyapplication, a low cost heat-pipe plate based thermal management system has been developed that canremove heat from solar panel with high efficiency has been developed. One of the key features of the deviceis the fluidic channels with nano-coated porous materials for effective capillary flow. The system has beenintegrated with a plasmonic amorphous silicon solar cell and it can dramatically recover the efficiency loss due

to temperature rise and thus increase the energy yield of the solar cells.


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23. Invited 3D PRINTING MICROFLUIDIC DEVICES – WHICH PRINTER TYPE?

Niall Macdonalda, Feng Lib, Joan Marc Cabota, Sidra Waheeda, Vipul Guptaa, Petr Smjekalb, Rosanne

Guijtc, Brett Paulla,b and Michael Breadmorea,b

a ARCCentre of Excellence for ElectromaterialsScience (ACES), School of Physical Sciences, University ofTasmania, Tasmania, Australia

b Australia Centre for Research on Separation Science, School of Physical Sciences- Chemistry, Universityof Tasmania, Tasmania, Australiac Pharmacy, School of Medicine, University of Tasmania, Tasmania, AustraliaCorresponding Author: [email protected]

Despite being an old technique, additive manufacturing, or 3D printing as it has more commonly becomeknown, has received significant attention because of the possibility to create just about anything, from guns,cars, body parts and designer fashion items. It also shows much promise in the field of microfluidics wherethe possibility to go from computer design to physical microfluidic device in hand within a few hours wouldsignificantly change the field. While this is not quite reality, devices that can be produced with 3D printersdemonstrate the potential of this fabrication approach, but this depends on the type of printer selected for use.This presentation will discuss the potential of inkjet, fused deposition modelling and stereolithography printersfor the fabrication of microfluidic devices, and will address their advantages and disadvantages of each

approach to help choose the right printer to make the devices right for you.

24.SELF-PROPELLED MOTION AND EVAPORATION OF A FLOATING LIQUID MARBLE

Chin Hong Ooi, Nam-Trung Nguyen*

QLD Micro and Nanotechnology Centre, Griffith University, Nathan Campus, 170 Kessels Road, Nathan

QLD 4111, Australia.* Corresponding Author: [email protected]

A liquid marble is a liquid droplet coated with hydrophobic powder. The hydrophobic coating separates theenclosed liquid with its surroundings. This feature allows a liquid marble to float on liquid surfaces with the helpof surface tension. The liquid marble has been used as a microbioreactor or a three-dimensional cell growthplatform.1 A mobile flating liquid marble could serve as a digital microfluidics platform. A liquid marblecontaining aqueous ethanol can propel itself across a water surface because of the Marangoni solutocapillaryeffect.2 Recently, we showed that the floating liquid marble motion depends on the ratio of the water surfaceto liquid marble size and the ethanol concentration of the liquid marble.3 This was done by tracking the motionof a floating liquid marble. As the liquid marble coating is porous, the liquid content evaporates. The lifetime ofthe liquid marble depends on its evaporation rate. We investigated the evaporation rate of a sessile liquidmarble on a solid surface that contains aqueous ethanol. We analysed the changes in volume, mass, densityand surface tension via a combination of direct measurement and marble surface profile fitting. The

evaporation rate of a liquid marble differs from that of an aqueous ethanol droplet because the powder coatingsignificantly affects its critical properties.

References1. Sarvi, F.; Jain, K.; Arbatan, T.; Verma, P. J.; Hourigan, K.; Thompson, M. C.; Shen, W.; Chan, P. P. Y.; Adv.

Healthc. Mater. 2014, 77-86. 2. Bormashenko, E.; Bormashenko, Y.; Grynyov, R.; Aharoni, H.; Whyman, G.; Binks, B. P. J. Phys. Chem. C

2015, 119, 9910-9915. 3. Ooi, C. H.; Nguyen, A. V.; Evans, G. M.; Gendelman, O.; Bormashenko, E.; Nguyen, N. T. RSC Adv., 2015,

5, 101006-101012.



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25.EXTRACTION AND ‘SCALE-UP’ OF PLATINUM USING MICROFLUIDIC SOLVENT EXTRACTION

Frederik H. Kriel1, Craig Priest*1, John Ralston1, Luke Parkinson1, Stephen Woollam2, Neville Plint2, Richard

A. Grant3, Peter Ash3

1 Future Industries Institute, University of South Australia, Mawson Lakes, Australia2 Anglo American’s Technical Solutions, Johannesburg, South Africa

3 Johnson Matthey Technology Centre, Sonning Common, Reading, United Kingdom* Corresponding Author: [email protected]

Microfluidic technologies have transformed the way tiny volumes of fluid are dispensed, transported, reacted,and separated in chemistry and biology applications, and, while higher throughput processing has shown greatpromise, major challenges exist at these scales. Traditional ‘scale-up’ is not possible due to the high surface -to-volume ratio being a key feature of the system and, therefore, massive parallelization is necessary whilemaintaining flow stability and efficient phase disengagement. We have shown that microfluidic solventextraction (SX) can be applied to mineral processing, using model, particle-spiked, and real leach solutions.1-4 In the chip, two channels (tens of µm high and wide etched in Pyrex glass) merge to contact the aqueous and

organic phases for periods from milliseconds up to 1 min before diverging. Here, we present the first study ofmicroSX in a multichip configuration for precious metals refining, focusing on extraction performance, flowstability, and throughput for platinum by anion exchange extractants. The results suggest that parallel operationof these multi-chip modules can be reliably achieved and may be further ‘numbered-up’ for industrial use.

References1. Priest, C., et al., Chemical Engineering & Technology 2012, 35, 1312-13192. Priest, C., et al., International Journal of Mineral Processing 2011, 98, 168-173.3. Priest, C., et al., J. Flow Chem. 2013, 3, 76-80.4. Kriel, F.H., et al., Chemical Engineering Science 2015, 138, 827-833



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26.EXPLORING COLOURED MATERIALS FOR APPLICATION IN CHEMILUMINESCENCE DETECTIONFLOW CELLS AND MICROFLUIDIC CHIPS

Kara B. Spilstead1, 2*, Richard Alexander 2, Egan H. Doeven2, Paul S. Francis1, Stephen J. Haswell2, Neil W.Barnett1

1 Deakin University, Geelong, Australia. School of Life and Environmental Sciences, Centre for Chemistry

and Biotechnology, (Waurn Ponds Campus). 75 Pigdons Road, Geelong, VIC 3220, Australia2 Deakin University, Geelong, Australia. School of Life and Environmental Sciences, Centre for Regional andRural Futures, (Waurn Ponds Campus). 75 Pigdons Road, Geelong, VIC 3220, Australia* Corresponding Author: [email protected]

The use of coloured materials for chemiluminescent flow cell detection has not extensively been explored todate. In the late 1970s, Stieg and Nieman compared the use of white and black materials forchemiluminescence flow cells in flow injection analysis, and demonstrated that white cells produced signalintensities that were far superior.1 Since this initial work, however, chemiluminescence detectors constructedby machining channels have almost exclusively been prepared using transparent materials, 2-5 and there hasbeen little exploration of other coloured materials, even though they could provide an advantage in thedetection of low concentration samples.

This work examines the application of a range of coloured materials to enhance detection using differentchemiluminescence reactions (luminol and permanganate) in microfluidic sized channels. The effectivenessof each cell has been evaluated by comparing the chemiluminescence intensity of continuously mergedreactants, as well as the distribution of light throughout the reaction zone using photographic image analysiswith ImageJ software. Results demonstrate that a red cell produces 3-5 times greater signal intensities thanblue cells for red emitters, and so there is a potential for coloured materials to be incorporated into flow cellsand microfluidic chips.

References1. S. Stieg and T. A. Nieman, Analytical Chemistry , 1978, 50, 401-404.2. A. Economou, A. K. Clark and P. R. Fielden, Analytical Communications, 1998, 35, 389-390.3. L. M. Magalhães, M. A. Segundo, S. Reis, J. L. F. C. Lima, J. M. Estela and V. Cerdà, Analytical Chemistry ,

2007, 79, 3933-3939.4. J. M. Terry, J. L. Adcock, D. C. Olson, D. K. Wolcott, C. Schwanger, L. A. Hill, N. W. Barnett and P. S.Francis, Analytical Chemistry , 2008, 80, 9817-9821.

5. B. Kuswandi, Nuriman, J. Huskens and W. Verboom, Anal. Chim. Acta, 2007, 601, 141-155.



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27. Keynote TRAJECTORIES AND CHARGE IN TUNABLE RESISTIVE PULSE SENSING

Geoff R. Willmott*1,2, Eva Weatherall1,3 and Peter Hauer 1,3

1The MacDiarmid Institute for Advanced Materials and Nanotechnology2 Departments of Physics and Chemistry, The University of Auckland, New Zealand3School of Chemical and Physical Sciences, Victoria University of Wellington, New Zealand


Tunable resistive pulse sensing (TRPS) is a pore-based technique which enables high throughput, particle-by-particle analysis of submicron colloids.1 This presentation will focus on two extensions to the basic analysisusually used for TRPS concentration, size, and charge measurements. The work is important forunderstanding the precision and accuracy of TRPS, especially as the technique is applied over an increasingrange of experimental conditions. The results should also be relevant for resistive pulse sensing techniques ingeneral, and various similar nano- and microfluidic systems.

Firstly, we consider the pore surface charge, which controls electro-osmotic transport. Surface charge is alsoresponsible for non-uniform spatial distributions of ions, which can give rise to conductive pulses. 2 Ourexperiments study elastomeric polyurethane pores which have controlled surface chemistry, with electrokinetic

properties characterized using streaming potential measurements.Secondly, the occurrence and effects of particle trajectories have been studied using TRPS in conjunction withfluorescence microscopy.3 Correlations between sizes and durations of both resistive and fluorescence pulsescan be explained by considering trajectories, the laser beam shape, and the distribution of ions. Finite elementmethods have been applied to model both trajectories and ionic distributions.

References1. Weatherall, E.; Willmott, G. R. Analyst 2015, 140 , 3318-3334. 2. Weatherall, E.; Willmott, G. R. J. Phys. Chem. B 2015 119, 5328–5335. 3. Hauer, P.; Le Ru, E. C.; Willmott, G. R. Biomicrofluidics 2015, 9, 014110.

28. InvitedDEVELOPMENT OF A LAB ON A CHIP SYSTEM FOR THE DETECTION OF INFLUENZA VIRUS

Egan H. Doeven*, Richard Alexander, Yi Heng Nai, Stephen J. Haswell

Centre for Rural and Regional Futures, School of Life and Environmental Sciences, Faculty of Science,Engineering and Built Environment, Deakin University, Geelong, Victoria 3220, Australia. * Corresponding

Author: [email protected]

Lab on a chip (LOAC) systems have the potential to revolutionise the point of need diagnostics industry byproviding faster, cheaper and more comprehensive analytical data to the user. However whilst many LOAC

systems with widespread commercial applicability have been proposed, very few have made it to market, oftendue to devices relying on techniques or materials that prove difficult to manufacture on a large scale.

This research presents a concept LOAC system for the detection of avian influenza A viral RNA which hasbeen designed for manufacturability, whilst still providing quality data to the user. Injection moldable polymershave been used to manufacture the chip substrate, with isothermal nucleic acid sequence based amplification(NASBA) being used to amplify selected viral RNA. Detection of the amplified RNA is achieved usingelectrochemiluminescence, which can be achieved without an external excitation source or optics as requiredfor fluorescence based detection techniques. Reagent and solution storage / chip filling are handled via acombination of freeze drying (for reagents) and blister storage / sequenced bursting (solution storage and chipfilling). Sample transport within the chip is achieved through the use of magnetic particles with tailored surfacefunctionality and a system of moveable magnets, negating the requirement for pumps or complex solution-chip interfaces.

This presentation will outline the strategies used in designing this microfluidic device which combines theinherent advantages of microfluidics with optimised manufacturability and optimal simplicity of operation. Datafrom prototype devices will show the viability of the approach taken.



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29.SELECTIVE LATERAL TRANSPORT OF PARTICLES AND SOLUTION EXCHANGE IN VISCOELASTICFLUID

Dan Yuan1, Jun Zhang2, Sheng Yan3, Gangrou Peng4, Qianbin Zhao5, Gursel Alici6 and Weihua Li*7,

1 School of Mechanical, Materials and Mechatronic Engineering, University of Wollongong, Wollongong,NSW 2522, Australia.* Corresponding Author: [email protected]

In this work a novel technique for selective lateral transport of micro-particles and solution exchange usingnon-Newtonian viscoelastic fluid is proposed. Micro-particles suspended in a PEO (polyethylene oxide)solution stream will migrate laterally to a DI (deionised) water stream, but transfer in the opposite directionfrom a DI water stream to a PEO solution stream or from one DI water stream to another DI water stream couldnot be achieved; this means that the lateral transportation of particles depends on the viscoelastic propertiesof the two co-flowing fluids. This result inspired an investigation into the forces experienced by particles inthese co-flowing fluids and the mechanisms inherent in this selective transport phenomenon, as well as howthe flow rate, PEO concentration, channel length, and type of solution affects the migration of these micro-particles. This technique of passive particle transfer can deliver a selective, rapid, high throughput (~10000/s)particle transfer and solution exchange by simply adding harmless PEO molecules to the particle suspension,without any external force field, in a simple straight channel. This selective transfer technique promotesautomated cell staining and washing in microfluidic platforms, and holds numerous applications for

biomedicine.


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30. Sponsor Talk # 2 ANFF – SOUTH AUSTRALIA: CHIPS AND INTERFACES FOR MICRO/NANOFLUIDICS

Craig Priest1, 2

1 South Australian Node of the Australian National Fabrication Facility, University of South Australia andFlinders University, Australia2 Future Industries Institute, University of South Australia, Mawson Lakes, Australia

Corresponding Author: [email protected]

The South Australian Node of the Australian National Fabrication Facility (ANFF-S) was established in 2007under the Australian Government’s National Collaborative Research Infrastructure Strategy (NCRIS). SA’snode is located at UniSA’s Mawson Lakes Campus and Flinders University’s Bedford Park Campus, and ishome to world-class clean room facilities and a suite of equipment related to interfacial coating, patterning,and structuring. Technical staff work closely with research and industry users to design, fabricate,package/interface, and prototype fluidic devices for diverse purposes, from water quality tobioassay/separations to mineral processing. The ANFF-SA team are specialists in silica/borosilicate glass andsilicon microchips, prepared by plasma and wet etching processes. Polymer chips can also be prepared usinghot embossing, direct micro-machining, and moulding. Value-adding to your microchips by includingelectrodes, sensors, and optical components is also possible, customised for your specific design.

Fig. 1 (a) Plasma-etched borosilicate glass (b) custom chip holders and fluid interfacing, and (c) chip withembedded electrodes for on-chip electrochemistry.

31. Sponsor Talk # 3 THE QUEENSLAND MICROTECHNOLOGY FACILITY – INNOVATION CENTRE AND CAPABILITIES

Alan Iacopi1and Nam-Trung Nguyen1

1QLD Micro and Nanotechnology Centre, Griffith University, Nathan Campus, 170 Kessels Road, NathanQLD 4111, Australia


Bridging the gap between university and industry, the Queensland Microfabrication Facility provides afabrication capability using standard industry silicon wafer processing techniques for R+D, prototyping and lowvolume production.

The focus of the facility is on applied research into SiC technologies for both epitaxial grown SiC on Si andSiC semiconductor devices fabricated on SiC wafers. Over the following decades, SiC will be increasing usedin the commercialization of next generation products. Some of the attributes of SiC and process capability willbe presented and how these can be translated into viable products capable of exploitation in Australia.

The facility, part of the Australian National Fabrication Facility, provides a process innovation centre for Australia for High Value Manufacturing. The industrial partnerships that have been established enable us to

be one of the few centres in worldwide able to capitalise on SiC technology. We are the only facility that canprovide epitaxial SiC growth on Si for up to 300mm dia silicon wafers. The open innovation model enablesclients to develop products within the facility without capital expenditure . We provide expertise so our

customers can innovative, demonstrate processes and support product feasibility for commercialisation.

(a) (b) (c)



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32. Keynote ANDROID VOLTAMMETRY: LOW COST ELECTROCHEMICAL DETECTION FOR PAPERMICROFLUIDIC SENSORS USING A MOBILE DEVICE

Conor F. Hogan,*1 Darrell Elton,2 Seng Loke,3 Kiran Bano1

1 La Trobe Institute for Molecular Science, Department of Chemistry and Physics; 2 Dept. of ElectronicEngineering, 3 Computer Science & Computer Engineering, La Trobe University, VIC 3086, Melbourne,

Australia. * Corresponding Author: [email protected]

The rapid expansion of mobile phones and other mobile technologies is set to transform the biosensinglandscape. In particular the widespread availability of smartphone technology and the capabilities they offerin terms of computation, communication, networking, and imaging will allow a more extensive deployment oflab-on-a-chip and related sensing technologies. Furthermore the combination of mobile technologies with low-cost sensing concepts such as paper microfluidics could make game-changing health and environmentaltesting technologies available to many millions more people both in the developed and developing worlds.Voltammetry is the cornerstone technique of electrochemical sensing, and almost all dynamic electrochemicalmethods can be regarded as variations of the basic voltammetric method. We will show for the first time thatquantitative voltammetric analysis may be carried out using only the intrinsic hardware in a mobile phone ortablet and a suitable software application, with no external device or instrument whatsoever. We call this newapproach Android voltammetry.



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Chair: Prof Leslie Y. Yeo, RMIT University

9.00 am Dr Craig Priest, University of South AustraliaInterfacial Effects on Fluids in Small Geometries

9.30 am A/Prof Chamindie Punyadeera, Queensland University of TechnologyIsolation, Culture and Characterization of Live Head and Neck Cancer Patient Derived CirculatingTumour Cells

9.50 pm Dr Richard Alexander , Deakin UniversityDesign and Fabrication of Commercially Viable Microfluidic Devices

10.10 pm Dr Tuncay Alan, Paul Scherrer Institut Xray Compatible Microfluidics for In-Situ Crystallization Studies


Chair: Dr Craig Priest, University of South Australia

11.00 am Prof Weihua Li, University of WollongongDielectrophoretic Microfluidic Devices for Manipulation of Particles

11.30 am Dr Marco Rasponi, Politecnico Di MilanoCyclic Uniaxial Strain on 3D Microconstructs: A Beating Heart-on-a-Chip Platform for The Generationof Functional Cardiac Microtissues

11.50 am Dr Laura G. Carrascosa, University of QueenslandOn-Chip Profiling of Clinically Relevant Exosomes Using a SPR Biosensor

12.10 pm Dr Mazher I. Mohammed, Deakin University Autonomous Microfluidics with Embedded Optics for Rapid Quantitative Environmental Analysis ofNitrates

12.30 pm LUNCH

Chair: Prof Weihua Li, University of Wollongong

1.15 pm Dr Rosanne M Guijt, University of Tasmania25 Year µTAS

1.45 pm Dr David W. Inglis, Macquarie UniversityDevelopments in Low Reynolds Number Passive Particle Separation

2.15 pm Dr Khashayar Khoshmanesh, RMIT UniversityLiquid Metal Based Fluidic Actuators

2.35 pm Dr Say Hwa Tan, Griffith University Developing a Versatile Microfluidic Tool for High-Throughput Drug Screening

Chair: Dr Muhammad J. A. Shiddiky, Griffith University

2.55 pm CLOSING PLENARYProf Leslie Y. Yeo, RMIT University

Microfluidic Nebulisation of Nanoaerosols and Nanoparticles for Inhaled Gene Delivery3.35 pm POSTER AWARDS

3.45 pm CONFERENCE CLOSE

DAY 3, 23 March 2016, WEDNESDAY


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33. Keynote INTERFACIAL EFFECTS ON FLUIDS IN SMALL GEOMETRIES

Craig Priest*1

1Future Industries Institute, University of South Australia, Mawson Lakes, SA, 5095, Australia* Corresponding Author: [email protected]

It is well-known that the importance of interfacial phenomena is amplified in small geometries; however, thethermodynamic models for partial wetting – which rely on minimization of the global free energy of theinterfaces present – tend to fail in real-world scenarios. So-called ‘energy barriers’, where a local surfacefeature (bump, scratch, or patch) prevents a meniscus or three-phase contact line from advancing or receding,i.e. ‘pins’ it, are usually responsible. It follows that small geometries amplify the importance of pinning andother non-ideal wetting phenomena, making understanding these effects vital in many small-scale applications,including micro and nanofluidics. In this presentation, basic theory, along with an explanation of observeddepartures, will be given. Then, selected application examples where understanding these effects is crucialwill be presented, including enhanced wetting hysteresis1, wicking of liquid in microstructures2,3, stabilizationof co-flowing immiscible streams4, and spontaneous bubble release in microgravity5.

References

1. Forsberg, P. S. H.; Priest, C.; Brinkmann, M.; Sedev, R.; Ralston, J., Langmuir 2010, 26, 860-865. 2. Holzner, G.; Kriel, F. H.; Priest, C., Analytical Chemistry 2015, 87, 4757-4764. 3. Kriel, F. H.; Priest, C., Analytical Sciences 2016, 32 103-108.�

Documents

Anznmf 2016 Abstract Book