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Page 1 of 2 WB = Western Blot IHC = Immunohistochemistry Packaging: Liquid in PBS. Contains no preservative. Storage and Stability: Store at -20oC in undiluted aliquots; stable for up to six months after date of receipt. Avoid freeze/thaw cycles. Shipment: Domestic - Blue Ice; International - Blue Ice. For Research Use Only.

Anti- Collagen I α1 Telopeptide Sequence Catalog Number: 322-COLT Size: 100 µl Product Description: Rabbit polyclonal antibody Applications: WB: 1:1000 IHC: 1:100 Antigen: Synthetic peptide from the C-terminal region of human collagen I α1 telopeptide sequence. Species reactivity: The antibody has been directly tested for reactivity in human, mouse, rat and sheep. Based on the homology of the immunogen this antibody is expected to recognize the collagen I α1 polypeptide in all mammals, birds, and amphibians. Biological Significance: Collagen is an extracellular matrix protein that serves as a scaffold defining the shape and mechanical properties of many tissues and organs including skin, tendon, artery walls, fibrocartilage, bone and teeth. Type 1 collagen is the must abundant protein in mammals. Collagens are synthesized with N-terminal and C-terminal propeptides that are cleaved during maturation and secretion. After cleavage of the propeptides, the most N-terminal and C-terminal remaining sequences are known as telopeptides. Mutations in the collagen 1, alpha 1 gene (COL1A1) are known to cause osteogenesis imperfecta (aka brittle bone disease) (Byers 1989). Furthermore, mutations found in the fist 90 residues of the helical region of alpha 1 collagen have been implicated in the prevention or delayed removal of the procollagen N-propeptide leading to a combined osteogenesis imperfecta and Ehlers-Danlos syndrome (EDS) phenotype (Cabral et al., 2005).

Anti-Collagen I α 1, telopeptide

Left: Western blot of rat lung lysate showing specific immunolabeling of the ~ 140k collagen protein. Right: Immunostaining of formaldehyde-fixed fibrotic mouse lung tissue. The antibody recognizes mature collagen I (red) that has formed fibrils in the extracellular matrix.

Page 2 of 2 WB = Western Blot IHC = Immunohistochemistry Packaging: Liquid in PBS. Contains no preservative. Storage and Stability: Store at -20oC in undiluted aliquots; stable for up to six months after date of receipt. Avoid freeze/thaw cycles. Shipment: Domestic - Blue Ice; International - Blue Ice or Dry Ice. For Research Use Only.

Purification Method: Affinity purified Antibody Specificity: Specific for the ~ 140 kDa telopeptide portion of the collagen I α1 polypeptide. The antibody works well for immunohistochemistry on paraformaldehyde-fixed sections with a simple antigen-retrieval protocol (incubate slides for 20 minutes at 90º C in 10 mM sodium citrate (pH 6.0)/ 0.1 % Tween-20). Note that in paraffin sections of formaldehyde-fixed fibrotic mouse lung tissue, the antibody recognizes mature collagen I that has formed fibrils in the extracellular matrix.

Quality Control Tests: Western blots performed on each lot.

Product Specific References:

Reese C, Lee R, Bonner M, Perry B, Heywood J, Silver RM, Tourkina E, Visconti RP, Hoffman S. (2014) Fibrocytes in the fibrotic lung: altered phenotype detected by flow cytometry. Front Pharmacol. 2014 Jun 16;5:141.

Bains, SN, Tourkina E, Atkinson C, Joseph K, Tholanikunnel B, Chu HW, Riemer EC, Martin R, Hoffman S (2012) Loss of caveolin-1 from bronchial epithelial cells and monocytes in human subjects with asthma. Allergy. 2012 Dec;67(12): 1601-4.

Wessels A, van den Hoff MJ, Adamo RF, Phelps AL, Lockhart MM, Sauls K, Briggs LE, Norris RA, van Wijk B, Perez-Pomares JM, Dettman RW, Burch JB. (2012) Epicardially derived fibroblasts preferentially contribute to the parietal leaflets of the atrioventricular valves in the murine heart. Dev Biol. 2012 Jun 15;366(2): 111-24.

General References:

Byers PH (1989) Inherited disorders of collagen gene structure and expression. Am J Med Genet. 34(1):72-80.

Cabral WA, Makareeva E, Colige A, Letocha AD, Ty JM, Yeowell HN, Pals G, Leikin S, Marini JC. (2005) Mutations near amino end of alpha1(I) collagen cause combined osteogenesis imperfecta/Ehlers-Danlos syndrome by interference with N-propeptide processing. J Biol Chem. 2005 May 13;280(19):19259-69.