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8/10/2019 Anatomy and Physiology, Heart, Bp and Hematology Labs Moodle
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BIO1414 - Human A & P II
Heart, Blood Pressure & Hematology
Lab
Oklahoma City Community CollegeProfessor Steve Kash
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Lab Exam Objective 1 Given heart models & preserved heart
specimens, identify these structures: Right Atrium & Auricle
Right Ventricle
Left Atrium & Auricle
Left Ventricle Ascending Aorta & Aortic Arch
Brachiocephalic Trunk
Left Common Carotid & Left Subclavian Arteries
Pulmonary Trunk
Right & Left Pulmonary Arteries Right & Left Pulmonary Veins
Apex
Superior & Inferior Vena Cava
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Lab Exam Objective 1 continued Given heart models & preserved heart
specimens, identify these structures: Coronary Sinus & Sulcus
Interatrial & Interventricular Septum
Epicardium (Visceral Pericardium)
Endocardium & Myocardium
Parietal Pericardium (Pericardial Sac) Tricuspid (Right Atrioventricular) Valve
Bicuspid (Mitral) (Left Atrioventricular) Valve
Aortic & Pulmonary Semilunar Valves
Trabeculae Carneae
Chordae Tendineae
Papillary & Pectinate Muscles
Anterior & Posterior Interventricular Sulcus
Ligamentum Arteriosum & Fossa Ovalis
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Lab Exam Objective 1 continued Given heart models & preserved heart
specimens, identify these structures: R. Coronary Artery (RCA)
- Marginal Branch of RCA
- Posterior Interventricular Branch of RCA
L. Coronary Artery (LCA) - Anterior Interventricular Branch of LCA
- Circumflex Branch of LCA
- Marginal Branch of LCA
Great Cardiac Vein
Posterior Interventricular (Middle Cardiac) Vein
L. Marginal Vein
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Posterior Heart
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Anterior
Heart
Vessels
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Posterior
Heart
Vessels
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Aortic
Branches
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Heart ModelAnterior View
Left Pulmonary
ArteryLeft Pulmonary
Vein
Ascending Aorta
Great Cardiac VeinAnterior
InterventricularCoronary Artery
Superior Vena Cava
Aortic Arch
Pulmonary Trunk
Apex
Right Auricle
Right Ventricle Left Auricle
Left Ventricle
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Heart ModelPosterior View
R. Pulmonary Artery
R. Pulmonary Vein
Aortic Arch
Posterior
Interventricular Cardiac
Vein
PosteriorInterventricular
Coronary Artery
Superior Vena Cava
Coronary Sinus Inferior Vena Cava
Great Cardiac Vein
L. Marginal Cardiac
Vein
L. Pulmonary Artery
L. Pulmonary Vein
Apex
Right Ventricle
Left Ventricle
Left Atrium
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Heart ModelFrontal View
Left Atrium
Bicuspid (Mitral) ValveRight Atrium
Right Ventricle
Papillary Muscle
Chordae Tendineae
Left Ventricle
Tricuspid valve
Chordae Tendineae
Pulmonary TrunkPulmonary Semilunar Valve
Septum
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Preserved HeartAnterior View
Left Auricle
Left Ventricle
Right Auricle
Right Ventricle
Apex
Pulmonary Trunk
Aorta
Interventricular
Sulcus
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Preserved HeartFrontal View
Left Atrium
Bicuspid
(Mitral)
Valve
Right Auricle
Right Ventricle
Papillary Muscle
Chordae
Tendineae
Left Ventricle
Tricuspid valve
Chordae Tendineae
Ascending
Aorta
Aortic Semilunar
Valve
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Ci l ti
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3. Right Ventricle
2. Tricuspid
Valve
1. Right Atrium
Circulation
of O2/ CO2blood: heart
to lungs
4. Pulmonary
Semilunar
Valve
5. Pulmonary
Trunk
6. To Lungs via
Pulmonary Arteries
Ci l ti
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11. Aortic
SemilunarValve (not
shown)
12. Aorta
Circulation
of O2/ CO2
blood: lungsto heart
9. Bicuspid
(Mitral) Valve
8. Left Atrium
7. From Lungs via
Pulmonary Veins
10. Left
Ventricle
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Heart Circulation Summary
Superior & InferiorVena Cava
RA
Tricuspid Valve RV
Pulmonary Semilunar
Valve
Pulmonary Trunk
Pulmonary Arteries
Lungs
Lungs Pulmonary Veins
LA
Bicuspid (Mitral)Valve
LV
Aortic SemilunarValve
Aorta
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Lab Exam Objective 3
Know the normal values for blood
pressure (including the units) & use of
the terms systo l ic& diastol ic
pressures.
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Terms, Definitions & Units
Blood Pressure - force against arterialwalls per unit of area in mmHg
Systolic Pressure - highestarterial
pressure(averages 120 mmHg) Diastolic Pressure - lowestarterial
pressure(averages 80 mmHg)
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Lab Exam Objective 4
Given a blood pressure reading, calculate &
explain Pulse Pressure (PP) & Mean Arterial
Pressure (MAP).
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Pulse Pressure (PP)
PP = Systolic PressureDiastolic Pressure
Example: if a patients blood pressure =
140/80 mmHg
Then: 14080 = 60 mmHg
PP represents the difference between the
highest pressure & the lowest pressure
during the entirecardiac cycle
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Lab Exam Objective 5
Describe the procedure for taking blood
pressure, including the artery measured &
the functions of the sphygmomanometer &
stethoscope.
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BLOOD PRESSURE LAB
STETHOSCOPESPHYGMOMANOMETER
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SPHYGMOMANOMETER
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Sphygmomanometer
Gauge
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STETHOSCOPE
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No Sounds of Korotkoff
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1stSounds of
Korotkoff
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Sounds
Continue
Last Korotkoff Sounds
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Last Korotkoff Sounds
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First & Last Sounds of Korotkoff
Sounds of Korotkoff
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Sounds of Korotkoff
Bl d P S
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Blood Pressure Summary
BP is measured by auscul tat ion(listening)
at the brach ial arteryusing a stethoscope No sound is heard during laminarblood
flow (normal, quiet, smooth blood flow)
Increasing cuff pressure, constricts(closes) the brachial artery, preventing
blood flow (no sounds)
As air pressure in the cuff is lowered, thebrachial artery partially opens, creating
turbulent blood flow (heard as the sounds
of Korotkoff)
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Blood Pressure Summary
continued
1stKorotkoff sound is heard at the
systolic pressure value (cuff pressure =systolic pressure)
When air pressure in the cuff is lower
than the diastolic pressure, turbulent flow(sounds) disappears
The last Korotkoff sound is heard at the
diastolic pressure value (cuff pressure =diastolic pressure)
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Lab Exam Objective 6
Relate the effects of body position &
exercise on blood pressure, heart rate
& pulse pressure.
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Systolic Pressure
Remains fairly constant in spite of body
position changes
Systolic pressure: caused by LVpeak
ejection pressure
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Diastolic Pressure
Diastolic pressure is affected by gravity &tends to not remain constant when body
position changes
Diastolic pressure: caused by elasticrecoi lof the brachial artery wall
If the arm is positioned below the heart
level, diastolic pressure may increase If the arm is positioned above the heart
level, diastolic pressure may decrease
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Lab Exam Objective 7
Define hematocrit & describe how
hematocrit is obtained; interpret
hematocrit results & compare them to
normal male & normal female values.
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Hematocrit
Heparinized Capillary Tube
Sheep Blood
Critoseal
Centrifuge
Hematocrit Reader Card
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Sheep Blood
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Critoseal & Capillary Tubes
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Centrifuge
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Capillary Tube Position
CRITOSEAL END
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Hematocrit Reader Card
Hematocrit Reader Card
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Hematocrit Reader Card
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Normal Hematocrit Values
Male: 42 - 52% Female: 37 - 48%
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Lab Exam Objective 8
Describe the procedure for typing
human blood & interpret human blood
typing results.
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Blood Typing
Blood typing tray
Typing blood vials
Anti-A antisera (antibodies)
Anti-B antisera (antibodies)
Mixing stick
Blood Typing Tray
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Blood Typing Tray
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Blood Typing Blood Vials
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Blood Typing Antisera Vials
Bl d T i
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Blood Typing
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Clumping
Reaction
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Lab Exam Objective 9
Using a microscope, textbook & lab
materials, identify erythrocytes (RBCs),
leukocytes (WBCs), & thrombocytes
(platelets); know the normal male &female values for each.
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Blood Cell Identifications
Prepared Blood Slide
Microscope
Oil Immersion
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White Blood Cell Identification
L k t (WBC )
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Leukocytes (WBCs)
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Blood Sample Quiz
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Normal WBC Values
Granulocytes:
Neutrophils = 60 -
70%
Eosinophils = 2 - 4%
Basophils = 0.5 - 1%
Agranulocytes:
Lymphocytes = 25 -
33%
Monocytes = 3 - 8%
L b E R i
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Lab Exam ReviewKNOW THESE FOR THE BLOOD PORTION OF THE LAB
EXAM: WBC, RBC, & PLATELET ID & NORMAL RANGES (%)
GENERALBLOOD LAB PROCEDURES
DEVICE NAMES: HEMATOCRIT READER CARD, ETC.
NORMAL HEMATOCRIT %
RECOGNIZE: ANTIGEN/ANTIBODY
(AGGLUTINATION) REACTIONS DETERMINE BLOOD TYPE BASED ON AN
AGGLUTINATION PATTERN
BIO1414 LAB EXERCISE ONE & TWO
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DISPOSAL INSTRUCTIONS BIOHAZARD DISPOSAL CONTAINERS:
- Blood contaminated toothpicks
- Blood contaminated gloves
- Blood contaminated plastic typing trays
SHARPS DISPOSAL CONTAINERS:
- Glass capillary tubes
-----------------------------------------------------------------------------------
Place dissected hearts back into the tubs in the sinks
Do not put uncontaminated gloves or dissected heartsinto Biohazard containers
Clean & disinfect lab table surfaces with paper towels byspraying them with Amphy l solution & place used paper
towels into the regular trash containers
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BIO1414 - Heart, BP & Hematology Lab - END