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Analysis of Galveston and Port Aransas tarballs after
the Texas City Y oil spill
Stefanie PlunkettLiu Laboratory
Texas City Y Spill
• March 22, 2014 • in Galveston Bay• 168,000 gallons of marine residual oil
(RMG 380) released
What is in crude oil?
What is in crude oil?Aliphatic HydrocarbonsAromatic Hydrocarbons
Polycyclic Aromatic Hydrocarbons (PAHs)
TOXIC
Tarball Location
Evaporation, biodegradation, photoxidation, dissolution
Research Questions?
• Are the tarballs in Port Aransas and Galveston from the Texas City Y Spill?
• How did the hydrocarbon components of tarballs change?
• Do tarballs harbor unique bacterial community?
Objectives
• Determine if the tarballs were sourced from the Texas City Y spill
• Track changes in the hydrocarbon compounds of the tarballs over time and space
• Evaluate the bacterial communities associated with tarballs
METHOD: Tarball collection
Location Sample Time of collection
Galveston
GV1 6 daysGV2 6 daysGV3 6 daysGV4 11 monthsGV5 11 monthsGV6 11 monthsGV7 11 months
Port Aransas
PA1 10 daysPA2 10 daysPA3 10 daysPA4 3 monthsPA5 3 monthsPA6 3 months
13 samples
METHOD: Hydrocarbon analysisSilica gel fractionation
Aliphatics Aromaticsmi n
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19
mV0
25
50
75
100
125
150
175
200
225
250
275
mV
0
25
50
75
100
125
150
175
200
225
250
275 1検出器2011/ 07/ 25 14: 44: 58
Gas Chromatography –Mass Spectrometry (GC/MS)
Freeze-drying at -70ºC
All data is normalized to Hopane (17α(H)-21β(H)-hopane)
• Resistant to degradation by bacteria
• Resistant to photooxidation• Biomarker for oil
fingerprinting
Concentration of X Concentration of Hopane
Hopane ratio
trisnorhopane norhopane 22S-homohopane 22R-homohopane 0
0.2
0.4
0.6
0.8
1
1.2
1.4
1.6
RMG 380 GV1 GV2 GV3 PA1 PA2 PA3 PA4 PA5 PA6 GV4 GV5 GV6 GV7
Hop
ane
Rat
io
Fingerprinting the Tarballs using Hopanoids
Location Sample Time of collectionPA1 10 daysPA2 10 daysPA3 10 daysPA4 3 monthsPA5 3 monthsPA6 3 monthsGV1 6 daysGV2 6 daysGV3 6 daysGV4 11 monthsGV5 11 monthsGV6 11 monthsGV7 11 months
Port Aransas
Galveston
Aliphatic Hydrocarbons
PAHs Analysis
n-alkanes
P-values C14 to C17: 0.031, 0.001, 0.028, 0.046
C9
C10
C11
C12
C13
C14
C15
C16
C17
Pristane
C18
Phytane
C19
C20
C21
C22
C23
C24
C25
C26
C27
C28
C29
C30
C31
C32
C33
C34
C35
C36
C37
C38
C39
C40
0
2
4
6
8
10
12
RMG 380 GV 1-3 PA 1-3
Hop
ane
Rat
io
C9 C10 C11 C12 C130
1
2
3
4
5
6
7
RMG 380 GV 1-3 PA 1-3
Hop
ane
Rat
io
C14 C15 C16 C170
1
2
3
4
5
6
7
8
9
RMG 380 GV 1-3 PA 1-3
Hop
ane
Rat
io
Naphth
alene
Biphen
yl
Acyna
pthyle
ne
Acena
pthen
e
Fluoren
e
Dibenz
othiop
hene
Phena
nathr
ene
Anthrac
ene
Fluoran
thene
Pyrene
Benz[a
]anthr
acen
e
Chryse
ne
Benzo
[b]flu
oranth
ene
Benzo
[k]flu
oranth
ene
Benzo
[a]py
rene
Benzo
(e)py
rene
Inden
o[1,2,
3-cd]p
yrene
Dibenz
[a,h]a
nthrac
ene
Benzo
[g,h,i
]peryl
ene
Perylen
e0
0.5
1
1.5
2
2.5
RMG 380 GV PA
Rat
io N
orm
aliz
ed to
Hop
ane
Polycyclic Aromatic Hydrocarbons (PAH)
C1-Nap
hthale
ne, 2
C1-Nap
hthale
ne, 1
C2-Nap
hthale
ne, 2
,6
C2-Nap
hthale
ne, 1
,6
C2-Nap
hthale
ne, 1
,2
C3-Nap
hthale
ne, 2
,3,5
C1-fluo
rene,
1
Dibenz
othiop
hene
C1-Dibe
nzoth
iophe
ne, 4
C1-Phe
nanth
rene,
3
C1-Phe
nanth
rene,
2
C1-Anth
racen
e, 2
C1-Phe
nanth
rene,
9
C1-Phe
nanth
rene,
1
C2-Dibe
nzoth
iophe
ne, 4
,6
C2-Phe
nanth
rene,
1,7
C1-Fluo
ranthe
ne, 3
Retene
C1-Pyre
ne, 1
C1-Pyre
ne, 4
C1-Chry
sene
, 3
C1-Chry
sene
, 60
1
2
3
4
5
6
7
RMG 380 GV PA
Rat
io N
orm
aliz
ed to
Hop
ane
Alkylated PAHs
Next-generation sequencing (Illumina)
Bacterial community analysis
DNA ExtractionTarballPeripheral sand
Sand Sample 1 Sand Sample 2 Sand Sample 3 PA1 PA2 PA30
0.1
0.2
0.3
0.4
0.5
0.6
0.7
0.8
0.9
TARBALLS
Rel
ativ
e C
ompo
sitio
n
PERIPHERAL SAND
Port Aransas bacterial community
Vibrionales
Oceanospirillales
Alteromonodales
Conclusions• Samples Gv1,2,3 from Galveston and PA 1, 2, 3
from Port Aransas were from Texas City Y Oil Spill
• LMW aliphatics and aromatics were dissipated in these tarballs likely due to evaporation
• Tarballs pose potential toxicity and could harbor pathogenic bacteria such as Vibrionales.
QUESTIONS