African horse sickness - Home: OIE - World Organisation … · Web viewEvaluation of several Complement Fixation Test Antigens and a RBT antigen for Serological Diagnosis of Glanders

OIE Collaborating Centre Reports

Activities in 2013

Title of Collaborating Centre: Zoonoses in Europe

Address of Collaborating Centre: Friedrich-Loeffler-InstitutFederal Research Institute for Animal Health

HeadquartersSüdufer 10

17493 Greifswald – Insel RiemsGERMANY

Tel.: +49(0)38351 71102

Fax: +49(0)38351 71151

e-mail address: [email protected]

website: www.fli.bund.de

Name of Director of Institute (Responsible Official):

Prof. Dr. Dr. h.c. Thomas C. Mettenleiter

(President of the FLI)

Name (including Title and Position) of Head of the

Collaborating Centre (formally OIE Contact Point):

Prof. Dr. Dr. h.c. Thomas C. Mettenleiter

(President of the FLI)

Name (including Title and Position) of writer of this report

(if different from above)

Dr. Jens Schell

(Research Coordination)

Annual reports of OIE Reference Centres, 2013 1

Zoonoses in Europe

Summary of activities specifically related to the mandate of OIE Collaborating Centres

ToR: To provide services to the OIE, in particular within the region, in the designated specialty, in support of the implementation of OIE policies and, where required, seek for collaboration with OIE Reference Laboratories

ToR: To identify and maintain existing expertise, in particular within its region

1. Activities as a centre of research, expertise, standardisation and dissemination of techniques within the remit of the mandate given by the OIE

Disease control Epidemiology, surveillance, risk assessment, modelling Training, capacity building Zoonoses Wildlife Avian diseases Aquatic animal diseases Animal welfare Diagnosis, biotechnology and laboratory Veterinary medicinal products Vaccines Food safety Feed safety Other (Name the category: )

Remark: FLI’s main area of expertise regarding the OIE mandate is “Zoonoses”, however expertise exists also concerning the other marked areas.

Centre of research: The Friedrich-Loeffler-Institut (FLI) is a higher independent Federal authority with the legal task to perform research on infectious diseases of animals. It houses all national reference laboratories (NRL) for notifiable infectious diseases of animals in Germany as well as seven OIE reference laboratories (OIE-RL) of which five concern zoonoses: Avian Influenza, Newcastle Disease, Bovine Herpesvirus-1 Infection, Brucellosis, Chlamydiosis, Glanders, and Rabies. FLI runs a Collaborating Centre of the WHO for rabies. Furthermore it is designated as a reference centre of the UN Food and Agriculture Organization (FAO) for influenza in animals and Newcastle disease as well as classical swine fever. FLI is also the national authority to give market authorization for diagnostic tests for infectious diseases in animals and participates in epidemiological investigations of animal disease outbreaks.

Zoonooses (Viral Infections)

Title of activity Scope

Animal Influenzaincl. A/H1N1pdm:

Passive surveillance of influenza virus infections in populations of domestic swine in the Northwest of Germany revealed cocirculation of at least for stable lineages including a reassortant between A/H1N1pdm and enzootic porcine influenza viruses (H1pdmN2). Increasing antigenic diversity among circulating strains was evident (Harder et al., 2013).

Avian Influenza (AI):

Research on new vaccines targeting the highly pathogenic avian influenza virus (HPAIV) epizootic in Egypt have been taken up. Work of further characterization of recent circulating HPAIV H5N1 and H9N2 AIV strains from Egypt is ongoing.Pathogenesis studies in chickens, pigeons and ferrets with Chinese origin A/H7N9 virus have been conducted in comparison to low pathogenic European origin H7N7 AIV indicating that also European H7 LP viruses harbour some potential to infect mammalian species.

2 Annual reports of OIE Reference Centres, 2013

Zoonoses in Europe

Crimean-Congo-Haemorrhagic-Fever (CCHF)

Recombinant N-Proteins of CCHFV strains coming from European, Asian and African origin were produced and diagnostic assays for the detection of specific antibodies in small and large ruminants have been developed and validated using reference sera. Comparative studies including the in-house ELISA, the commercial Vectorbest CCHF ELISA, which is based on inactivated full virus antigens, and a commercial immunofluorescence assay have been performed.Cattle sera from Macedonia and Albania were analysed and antibody prevalences were observed. Further CCHFV antibody testing of sera from additional Balkan countries is foreseen. Moreover, collaboration with partners in Mauritania, Sierra Leone, Cameroon and DR Congo was initiated. A total of 6000 domestic animal sera were received and analysis of these sera has started.In addition to working on the serological test quantitative RT-PCRSs for the detection of CCHFV-RNA were further optimized.

Japan-Encephalitis: Immunization of rats and mice with a human vaccine and recombinant glycoprotein (E protein) for generation of monoclonal antibodies. The antibodies will be selected for specific binding to JEV with minimal cross reactivity to viruses from the same serogroup (e.g. West Nile virus, Tick-borne encephalitis virus etc.). Development of ELISA based serological diagnostic systems for differentiation of JEV infection from infections with other Flaviviruses.

Nipah/Hendravirus infections:

The qRT-PCR assays for the diagnosis of Henipa virus infections were validated and can be applied for the analysis of diagnostic samples.Polyclonal and monoclonal antibodies against functional proteins of the hendra virus were generated. A second validation study of an indirect in-house antibody ELISA based on the N-protein is ongoing using a larger set of samples including sera from pigs experimentally challenged with the Nipah virus.

Orthopoxvirus infections

Several cases of orthopoxvirus infections in animals were diagnosed and 3 different cowpox viruses (CPXV) could be isolated and characterized (2 isolates from Alpacas and 1 isolate from a cat).More than 2300 samples from wild rodents (voles) were screened for orthopoxvirus-specific DNA.A method for full-length sequencing of CPXV was established using next generation sequencing. More than 10 different cowpox virus isolates from different animal species were full-length sequenced.

Parapoxvirus infections

Several cases of parapox virus infections (Orf) could be confirmed.

Rift-Valley fever : In 2013 the monitoring studies from a RVFV outbreak in Mauretania 2010 were finished and showed the involvement especially of small ruminants and camels in RVFV infection and transmission The studies were supported by a novel Gn based indirect ELISA. In addition, a set of 45 monoclonal antibodies against structural proteins of RVFV were generated and characterized. They will be used and provided for several approaches to enable and facilitate antigen detection assays (competitive ELISAs, indirect immunofluorescence, immunohistochemistry). The previous work demonstrates the preparedness for monitoring studies in Europe and will alleviate further studies on vector competence and susceptibility of European lifestock to RVFV in close collaboration with Dr. Noël Tordo (OIE Reference Centre for Viral Haemorrhagic Fevers, Paris).

Schmallenberg virus infection

More than 2000 samples of domestic ruminants and wild life were tested for SBV-antibodies and virus. Semen samples of bulls were screened for SBV-RNA. Selected SBV-strains were full-length sequenced and phylogenetically typed. PCR-systems for the diagnosis of SBV especially in semen samples were developed and validated including 2 international ring trials.More than 15 000 midges were screened for SBV-RNA.


Zoonoses in Europe

There are members of the Simbu-serogroup which are known to be zoonotic, however, for SBV it is confirmed that no zoonotic potential exists.

Usutu virus infection

The molecular biological and serological monitoring in wild birds was continued in close collaboration with different German bird clinics. Investigation of dead wild birds in close collaboration with the local state veterinary laboratories and with the Bernhard-Nocht-Institute for Tropical Medicine in Hamburg to detect USUV infected birds.

Viral Equine Encephalitis (VEEV, WEEV, EEEV):

Establishment of hybridoma cell lines kindly provided by J. Roehrig, CDC, and inclusion of produced monoclonal antibodies in the ongoing development of new ELISA based diagnostic systems. Further characterisation of these antibodies and investigation of their ability to bind to recombinant glycoproteins. Production of recombinant alphaviral glycoproteins in eucaryontic cells (BHK-21) for the use of glycosylated proteins in diagnostic sytems

West Nile Fever: Further development of different diagnostic tools for molecular biological detection (Pan-Flavi-PCR, microarray) of WNV infections in birds and horses. Different micro-neutralisation tests as a method to detect the antibody cross reactivities between the Flavivirus family members were performed. Virological and serological monitoring studies of wild birds in the last year. Differential diagnostic analysis on request of third parties.

Rabies The OIE-RL leads a core group of the Partners for Rabies Prevention (PRP) dedicated to develop a Blueprint for Bat Rabies Prevention.The OIE RL participated (i) in the 2013 annual proficiency testing regarding quality assessment of rabies serological testing for dogs and cats within the frame of the EU pet travel scheme, (ii) the 2013 inter-laboratory comparison test on FAT, RTCIT, conventional and RT-PCR, and (iii) an inter-laboratory comparison among the network of OIE-RL for rabies of the direct rapid immunohistochemical test (dRIT) for diagnosis of rabies.

Zoonooses (Bacterial Infections)


Bovine tuberculoses, Para tuberculoses

Research is focussing on improved and novel strategies for diagnosis and control of diseases (mycobacterial infections?).In the frame of the project “Mycobacterium bovis in the wildlife-livestock-human interface in East and Southern Africa.” (DFG Program: African network building for the control of neglected zoonoses) mycobacterial from livestock and wildlife (113 from Tanzania, 44 from Kenya” were sequence-analyzed for species identification samplesOngoing research project are “Integrated control of neglected zoonoses: improving human health and animal production through scientific innovation and public engagement” (EU FP7 – 221948; ICONZ) and “Development of novel diagnostic strategies for the ante-mortem immunodiagnosis of bovine tuberculosis and Johne´s disease (EMIDA ERA-Net, MYCOBACTDIAGNOSIS)”.

Brucelloses DNA positive control material for PCR and reference sera for brucellosis and B. ovis as well as B. ovis, B. canis and Yersinia enterocolitica O9 antigens have been provided to national and international laboratories. The laboratory carried out diagnostic testing for several OIE Member Countries (Spain, Egypt, Chile). Training courses for serological and molecular diagnostic techniques were performed for UAE, Pakistan and Egypt.

Chlamydioses Protocols and technical expertise of the RL's standard real-time PCR assays and DNA microarray tests were provided to laboratories in the Netherlands, Poland, Croatia, Ukraine and France. Researchers from the Netherlands, Poland and Ukraine were received in the laboratory for short technical visits. Chromosomal DNA aliquots from reference strains of Chlamydia spp. were shipped to labs in 8 different OIE member states (Ireland, Spain, France, the Netherlands, Ukraine, Poland and Australia). A total of 889 diagnostic tests on avian chlamydiosis) and 443 on ovine chlamydiosis were


Zoonoses in Europe

conducted to examine field samples sent in from other OIE countries.

EHEC Ongoing research project “Occurrence of EHEC O104:H4 genes in cattle herds from Germany and Spain” (EU ANTIGONE).Protocol and technical expertise of the reference laboratory standard STEC colony hybridization assay were provided to the laboratories in Spain (SaBio-IREC (CSIC-UCLM-JCCM), Ciudad Real, Spain; VISAVET Health Surveillance Centre, Universidad Complutense, Madrid, Spain).

Glanders An OIE-Twinning project with NRCE, Hisar, India was started. Training courses for serological and molecular diagnostic techniques were performed for Iraq. B. mallei - positive and negative control serum, DNA and LPS from B. mallei and B. pseudomallei strains was prepared and provided to other laboratories.

Tularemia Various nutrient media for culture of F. tularensis subsp. holarctica were compared with polymerase chain reaction (PCR) in a study with samples from wild animals (also published).A serological investigations of wild boars (Sus scrofa) and red foxes (Vulpes vulpes) as indicator animals for circulation of Francisella tularensis in Germany was performed and published.German Francisella tularensis isolates from European brown hares (Lepus europaeus) were typed using genetic and phenotypic markers in a cooperation with Sweden to elucidate their phylogeographic relation (also published).PCR assays were provided to Federal Laboratories and a ringtest was organized for the detection of F. tularensis using PCR assays with participants from Germany and Austria.

Zoonooses (Parasite Infections)


Dourine Dourine control sera were provided to the Laboratoire Régional d´Analyses et de Recherches, Casablanca Morocco,40 horse sera from Kyrgyzstan were examined for dourine antibodies

Echinococcosis A mathematical model for analysing the spatial and temporal distribution of Echinococcus multilocularis was further developed. The spread and behaviour of the raccoon dog (Nyctereutes procyonoides) as a new definitive host of Echinococcus multilocularis in eastern and central Europe was studied. A total of 33 foxes from Brandenburg, Germany, were tested for the presence of Echinococcus multilocularis by the Intestinal Scraping Technique. The parasite was detected in 15 samples. Molecular typing techniques (EmsB microsatellite analysis; detection of the mitochondrial markers cox1, nad1 and atp6) were established to study the spatial and temporal epidemiology of the infection of foxes with the parasite.A PhD student from Sudan was trained in study design, sampling and echinococcosis diagnosis. 33 out of 143 canine fecal samples from different regions in Sudan contained taeniid eggs, which were molecularly typed by multiplex PCR (Boubaker et. al., 2013). 535 sheep, 291 goats, 735 cattle and 430 camels were examined for hydatid cysts at slaughterhouses. While the prevalence was low in cattle (1.4%), sheep (0.3%) and goats (0.3%), a substantial proportion of camels was found infected (25.3%).

Zoonooses (Transmissible Spongiforme Encephalopathies, Prions)


Prions –TSE: Pathogenesis studies and risk assessment studies focus more on atypical BSE. Close collaborations with other NRLs and with research institutes were continued. BSE or scrapie positive samples and reference materials (e.g. fresh brainstem material or paraffin-embedded fixed tissue) were supplied to cooperating partners.


Zoonoses in Europe


Zoonoses in Europe

ToR: To propose or develop methods and procedures that facilitate harmonisation of international standards and guidelines applicable to the designated specialty

2. Proposal or development of any procedure that will facilitate harmonisation of international regulations applicable to the surveillance and control of animal diseases, food safety or animal welfare

Proposal title Scope/Content Applicable area

Harmonisation of diagnostic techniques on Echinococcosis

This study was done in the framework of APHAEA (harmonised Approaches in monitoring wildlife Population Health, And Ecology and Abundance) in collaboration with IREC, Spain, IZSTO, Italy, VetAgroSup, France, SVA Sweden, DTU, Denmark, FIWI at the University of Bern, Switzerland, the Artemis Research Institute, the Netherlands, IZSLER, Italy, and the University of Turin, Italy.

Surveillance and control of animal diseases Food safety Animal welfare

Real time PCR subtyping of swine influenza viruses

In order to cope with the increasing diversity of swine influenza viruses multiplex RT-qPCRs were developed to diagnose four hemagglutinin and two neuraminidase lineages including A/H1N1pdm in swine samples.


“Blueprint for Fox Rabies Prevention”

Completion and updating of the “Blueprint for Fox Rabies Prevention” (http://www.rabiesblueprint.com/)


Reliable detection method for Schmalenberg virus RNA in semen

Development and validation of PCR-system for the reliable detection of SBV-RNA in semen samples


In vitro production procedure of dourine antigen for CFT

The OIE-recommended method of production of Trypanosoma equiperdum whole cell antigen for diagnosis of dourine requires the infection of rats followed by harvest and purification the pathogen from blood. In vitro propagation of T. equiperdum saves animal lives and is expected to generate better standardized antigen for CFT and the opportunity to detect partial antigens of higher specificity to differentiate T. equiperdum from T. evansi.



http://www.rabiesblueprint.com/

Zoonoses in Europe

ToR: To establish and maintain a network with other OIE Collaborating Centres designated for the same specialty, and should the need arise, with Collaborating Centres in other disciplines

ToR: To carry out and/or coordinate scientific and technical studies in collaboration with other centres, laboratories or organisations

3. Did your Collaborating Centre maintain a network with other OIE Collaborating Centres (CC), Reference Laboratories (RL), or organisations designated for the same specialty, to coordinate scientific and technical studies?

Yes No

Name of OIE CC/RL/other organisation(s) Location Region of

networking Centre Purpose

National Veterinary Services Laboratories USDA, APHIS, Veterinary ServicesOIE-RL for Highly pathogenic avian influenza and low pathogenic avian influenza (poultry)OIE-RL for Viral Equine Encephalitis

Ames, USA

Africa Americas Asia and Pacific Europe Middle East

Avian Influenza: Organization and evaluation of an OFFLU ring trial on molecular detection and characterization of avian influenza viruses

Viral Equine Encephalitis: Development and validation of ELISAs based on recombinant protein as well as on full virus antigens

Centers for Disease Control & Prevention (CDC)WHO CC for Reference and Research on RabiesOIE RL for Rabies

Atlanta , USA


Rabies research, diagnostics, surveillance

Lethbridge LaboratoryNational Centre for Animal Disease (NCAD)Canadian Food Inspection AgencyOIE RL Bovine spongiform encephalopathy

Canada Africa Americas Asia and Pacific Europe Middle East

Prions – TSE:Diagnostic and Risk Assessment of BSE and atypical BSE, provision of ring trial samples to the Canadian NRL to support their quality control system. on cattle experimentally challenged with atypical BSE

Ottawa Laboratory Fallowfield (OLF), Canadian Food Inspection AgencyWHO CC for Control, Pathogenesis and Epidemiology of Rabies in CarnivoresOIE RL for Rabies

Nepean, Canada




http://www.oie.int/en/our-scientific-expertise/collaborating-centres/reference-centre-networks/

Zoonoses in Europe



ANSES - French Agency for Food, Environmental and Occupational Health & Safety

EU-RL for equine diseasesEU-RL for brucellosis.EU-RL for chlamydiosisWHO CC for Management on Zoonoses ControlOIE RL for Rabies

France Africa Americas Asia and Pacific Europe Middle East

West Nile virus:Successful participation in the proficiency test on WNV molecular diagnostics and serology organized by the EU-RLBrucellosis:Successful participation in the ring trial for production of B. ovis and porcine reference serum organized by the EU-RLChlamydiosis:Successful participation in the ring trial for production of B. ovis and porcine reference serum organized by the EU-RLSchmallenberg virus:Prevention and control of SBV (SBV-network)Rabies research, diagnostics, surveillance

WHO Collaborating Centre for “Arboviruses and Viral Haemorrhagic Fevers” at UPSA, Virology, Institute Pasteur,

Paris France


Rift-Valley-Fever virus:Studies on vector competence and susceptibility of European lifestock to RVFV

Institute of Virology and Immunology (IVI)

Mittelhäusern,

Switzerland


Differential diagnostics for animal diseasesWest Nile virus:Exchange of information on prevention and control of WNF infections.

WHO Collaborating Centre for Reference and Research on InfluenzaNational Institute for Medical Research - Mill Hill

London, United

Kingdom


avian Influenza: Receipt of A/H7N9 China 2013.

Rober Koch Institut Berlin, Germany


Brucellosis and Glanders:Successful participation in the ring trial to detect highly pathogenic agents including B.mallei/pseudomallei and rucella spp. (organized within the EU-project QUANDHIP; EAHC Grant Agreement No 2010 2102).

Japanese National Institute of Animal Health

Japan Africa Americas Asia and Pacific Europe Middle East

Prions – TSE:Comparative study on diagnostic of atypical BSE on cattle experimentally challenged with atypical BSE


Zoonoses in Europe



Animal Health and Veterinary Laboratories Agency (AHVLA)EU Reference laboratory for Transmissible Spongiform EncephalopathiesWHO CC for the Characterization of Rabies & Rabies-related Viruses lOIE RL for Rabies

United Kingdom


Brucellosis and Glanders:Successful participation in the international ring trial VETQAS CFT (organized by VLA Weybridge),Prions – TSE:discussions related to TSE diagnosticsbatch testing procedure of all BSE rapid tests: FLI is responsibel for the BioRad TeSeE testSchmalenberg virus:Prevention and control of SBV (SBV-network)Rabies research, diagnostics, surveillance

Reference Laboratory for Ovine Chlamydiosis, Institute of Veterinary Pathology, University of Zurich

Zurich, Switzerla

nd


Epidemiology of chlamydia infections in poultry: current activities are focused on aetiology, epidemiology and genetic diversity of the newly defined species of Chlamydia avium and Chlamydia gallinacea.

CODA-CERVA (Veterinary and Agrochemical Research Centre)

Belgium Africa Americas Asia and Pacific Europe Middle East

Schmalenberg virus:Prevention and control of SBV (SBV-network)

Central Veterinary Institute of Wageningen UR (CVI)

The Netherla

nds


Schmalenberg virus:Prevention and control of SBV (SBV-network)

Onderstepoort Veterinary InstituteOIE RL for Rabies

Onderstepoort, South Africa



4. Did your Collaborating Centre maintain a network with other OIE Collaborating Centres, Reference laboratories, or organisations in other disciplines, to coordinate scientific and technical studies?

Yes No

Remark: please see question 3

ToR: To place expert consultants at the disposal of the OIE.

5. Did your Collaborating Centre place expert consultants at the disposal of the OIE?

Yes No

Name of expert Kind of consultancy Subject

Prof. Franz Conraths Consultation with scientists from Sudan

Study design to assess the epidemiological situation regarding Echinococcus granulosus s.s.

Prof. Franz Conraths Consultation with the European Food Safety Agency

Risk assessment of the epidemiological situation regarding Echinococcus multilocularis in Europe.


Zoonoses in Europe

Name of expert Kind of consultancy Subject

Prof. Heinrich Neubauer

Contribution to the Terrestrial Manual Diagnostic Tests and Vaccines for Terrestrial Animals

Contribution to the brucellosis chapters


Contribution to the Terrestrial Manual Diagnostic Tests and Vaccines for Terrestrial Animals

Contribution to the glanders chapter


OIE ad hoc group meeting: Contribution to the Terrestrial Animal Code

Contribution to the glanders chapter

Prof. Heinrich Neubauer,Dr. Mandy Elschner

Visit at the Candidate Laboratory NRCE Hisar, India

OIE-Twinning project Glanders

Prof. Timm Harder Consultation with Ministry of Agriculture, North Korea

Avian influenza: control and prevention

Prof. Thomas Mettenleiter

OIE Scientific Commission for Animal Diseases

Strategies and measures for animal disease prevention and control

PD Dr. Martin Beer OIE ad hoc group meeting Schmallenberg virus: Invited expert (OIE, Paris 10.10.2013)

Prof. Martin Groschup Consultancy of FAO/IAEA Division of Nuclear Techniques in Food and Agriculture

Discussion on novel technologies for monitoring animal and zoonotic disease vectors, as well as detection of the pathogens in vectors, and vector host interactions.

Dr. Thomas Müller Partners for Rabies Prevention - Expert meeting

Rabies virus: Invited expert (PRP, Wolfsberg, Toronto)

Dr. Thomas Müller EU – NRL for Rabies meeting Rabies virus: Invited expert (EU, Athens)

Dr. Thomas Müller World Rabies Day - Expert Meeting

Rabies virus: Invited expert (Annecy)

Dr. Thomas Müller Contribution to the Terrestrial Manual Diagnostic Tests and Vaccines for Terrestrial Animals

Contribution to the rabies chapter

ToR: To provide, within the designated specialty, scientific and technical training to personnel from OIE Member Countries

6. Did your Collaborating Centre provide scientific and technical training, within the remit of the mandate given by the OIE, to personnel from OIE Member Countries?

Yes No

If the answer is yes, please provide the total number of trained persons for each of the following categories:

a) Technical visits:................................................................6

b) Seminars:.........................................................................

c) Hands-on training courses:..............................................25


Zoonoses in Europe

d) Internships (>1 month):...................................................18

Type of technical training provided

(a, b, c or d)Content

Country of origin of the expert(s) provided with

training

No. participants from the corresponding

country

a Technical visit from Glanders OIE-Twinning Candidate Laboratory NRCE, Hisar

India 2

a Bovine tuberculosis control in Germany.

Mongolia 1

a Crimean-Congo-Haemorrhagic-Fever: on site visit at the Mauritanian national reference laboratory (CNERV, Nouakchott)

Mauretania 2

a Provided training on rabies diagnostic test methods

Botswana 1

c Chlamydiosis: Technical training and consultations on diagnostic methods (i.p. cell culture techniques and molecular diagnostic methods)

Netherlands, PolandUkraine

211

c Training on microbiological, serological and molecular diagnostic of Glanders

Iraq 3

c Crimean-Congo-Haemorrhagic-Fever: diagnostic of CCHFV by quantitative RT-PCR and detection of CCHFV-specific antibodies in cattle sera by ELISA (3 training courses with varying participants)

EgyptSierra Leone

KameronDR Congo

Mauretania

22121

c Provided training on Schmallenberg virus diagnostics

China 2

c+d Training on molecular diagnostic of Brucellosis

UAE 2

c+d Training on molecular typing of Brucellosis

UAE 3

c+d Training on microbiological, serological and molecular diagnostic of Brucellosis

Pakistan 2

c+d Microbiological, serological and molecular diagnostic of Brucellosis

Egypt 3

d Study design, sampling and Echinococcosis diagnosis

Sudan 1

d Doctoral thesis on microbiological, serological and molecular diagnostic of Brucellosis in Egypt

Egypt 1

d Microbiological, serological and molecular diagnostic of Brucellosis

Turkey 1


Zoonoses in Europe

Type of technical training provided

(a, b, c or d)Content

Country of origin of the expert(s) provided with

training

No. participants from the corresponding

country

d Provided training on avian influenza virus diagnostics (classical and molecular techniques)

Botswana 1

d Master Thesis at the Berlin School of Public Health at Charité. Training in the of PCR screening of European bat samples for a variety of viruses, including paramyxoviruses.

Bangladesh 1

d Training program concerning immunohistochemical techniques in the detection of pathological prion protein (TSE-diseases).

Brasília 1

ToR: To organise and participate in scientific meetings and other activities on behalf of the OIE

7. Did your Collaborating Centre organise or participate in the organisation of scientific meetings on behalf of the OIE?

Yes No

ToR: To collect, process, analyse, publish and disseminate data and information relevant to the designated specialty

8. Publication and dissemination of any information within the remit of the mandate given by the OIE that may be useful to Member Countries of the OIE (Indicate in the appropriate box the number by category)

a) Articles published in peer-reviewed journals:.................350

Selected publications of the FLI:

Viral diseases:

Avian and Animal InfluenzaHarder, T.C., E. grosse Beilage, E. Lange, C. Meiners, S. Döhring, S. Pesch, T. Noé, C. Grund, M. Beer, and E. Starick. 2013. Expanded co-circulation of stable subtypes, emerging lineages and new sporadic reassortants of porcine influenza viruses in swine populations in Northwest Germany. J Virol 87:10460-10476.

Zhao, N., E. Lange, S. Kubald, C. Grund, M. Beer, and T.C. Harder. 2013. Distinction of subtype-specific antibodies against European porcine influenza viruses by indirect ELISA based on recombinant hemagglutinin protein fragment-1. Virology J 10:246.

Crimean-Congo-Haemorrhagic-Fever (CCHF):Mertens, M., K. Schmidt, A. Ozkul, and M.H. Groschup. 2013. The impact of Crimean-Congo hemorrhagic fever virus on public health. Antiviral Res 98:248-260.

Hanta disease:Klempa, B., T. Avsic-Zupanc, J. Clement, T.K. Dzagurova, H. Henttonen, P. Heyman, F. Jakab, D.H. Krüger, P. Maes, A. Papa, E.A. Tkachenko, R.G. Ulrich, O. Vapalahti, and A. Vaheri. 2013. Complex evolution and epidemiology of Dobrava-Belgrade hantavirus: definition of genotypes and their characteristics. Arch Virol 158:521-529.

Faber, M., T. Wollny, M. Schlegel, K.M. Wanka, J. Thiel, C. Frank, D. Rimek, R.G. Ulrich, and K. Stark. 2013. Puumala Virus Outbreak in Western Thuringia, Germany, 2010: Epidemiology and Strain Identification. Zoon Publ Health 60:549-554.

Rift-Valley fever :Jäckel, S., M. Eiden, M. Dauber, A. Balkema-Buschmann, A. Brun, and M.H. Groschup. 2013. Generation and application of monoclonal antibodies against Rift Valley fever virus nucleocapsid protein NP and glycoproteins Gn and Gc. Arch Virol [Epub


Zoonoses in Europe

ahead of print; doi:10.1007/s00705-013-1867-4]

Jäckel, S., M. Eiden, A. Balkema-Buschmann, M. Ziller, P. Jansen van Vuren, J.T. Paweska, and M.H. Groschup. 2013. A novel indirect ELISA based on glycoprotein Gn for the detection of IgG antibodies against Rift Valley fever virus in small ruminants. Res Vet Sci 95:725-730.

Schmallenberg diseaseWernike, K., M. Kohn, F.J. Conraths, D. Werner, D. Kameke, S. Hechinger, H. Kampen, and M. Beer. 2013. Transmission of Schmallenberg Virus during Winter. Emerg Infect Dis 19:1701-1703.Hoffmann, B., C. Schulz, and M. Beer. 2013. First detection of Schmallenberg virus RNA in bovine semen, Germany, 2012. Vet Microbiol 167:289-295.

Usutu diseaseGarigliany, M.-M., D. Marlier, K. Tenner-Racz, M. Eiden, D. Cassart, R. Gandar, M. Beer, J. Schmidt-Chanasit, and D. Desmecht. 2013. Detection of Usutu virus in a bullfinch (Pyrrhula pyrrhula) and a great spotted woodpecker (Dendrocopos major) in north-west Europe. Vet J [Epub ahead of print; doi:10.1016/j.tvjl.2013.10.017]

West Nile Fever:Ziegler, U., J. Angenvoort, D. Fischer, C. Fast, M. Eiden, A.V. Rodriguez, S. Revilla-Fernández, N. Nowotny, J. García de la Fuente, M. Lierz, and M.H. Groschup. 2013. Pathogenesis of West Nile virus lineage 1 and 2 in experimentally infected large falcons. Vet Microbiol 161:263-273.Ziegler, U., A. Skrypnyk, M. Keller, C. Staubach, M. Bezymennyi, A.M. Damiani, N. Osterrieder, and M.H. Groschup. 2013. West Nile Virus Antibody Prevalence in Horses of Ukraine. Viruses 5:2469-2482.

Rabies:Kgaladi, J., N. Wright, J. Coertse, W. Markotter, D. Marston, A.R. Fooks, C.M. Freuling, T.F. Müller, C.T. Sabeta, and L.H. Nel. 2013. Diversity and Epidemiology of Mokola Virus. PLoS Negl Trop Dis 7:e2511.Freuling, C.M., K. Hampson, T. Selhorst, R. Schröder, F.X. Meslin, T.C. Mettenleiter, and T. Müller. 2013. The elimination of fox rabies from Europe: determinants of success and lessons for the future. Phil Trans R Soc B 368:20120142.

Bacterial diseases:

Bovine tuberculosis, ParatuberculosisMwakapuja, R.S., Z.E. Makondo, J. Malakalinga, W. Bryssinckx, R.H. Mdegela, I. Moser, R.R. Kazwala, and M. Tanner. 2013. Prevalence and significant geospatial clusters of bovine tuberculosis infection at livestock-wildlife interface ecosystem in Eastern Tanzania. Trop Anim Health Prod 45:1223-1230.

Mwakapuja, R.S., Z.E. Makondo, J. Malakalinga, I. Moser, R.R. Kazwala, and M. Tanner. 2013. Molecular characterization of Mycobacterium bovis isolates from pastoral livestock at Mikumi-Selous ecosystem in the eastern Tanzania. Tuberculosis 93:668-674.

BrucellosisAli, S., Q. Ali, F. Melzer, I. Khan, S. Akhter, H. Neubauer, and S.M. Jamal. 2013. Isolation and identification of bovine Brucella isolates from Pakistan by biochemical tests and PCR. Trop Anim Health Prod [Epub ahead of print; doi:10.1007/s1250-013-0448-6]

Ali, S., Q. Ali, H. Neubauer, F. Melzer, M. Elschner, I. Khan, E.N. Abatih, N. Ullah, M. Irfan, and S. Akhter. 2013. Seroprevalence and Risk Factors Associated with Brucellosis as a Professional Hazard in Pakistan. Foodborne Pathog Dis 10:500-505.Karger, A., F. Melzer, M. Timke, B. Bettin, M. Kostrzewa, K. Nöckler, A. Hohmann, H. Tomaso, H. Neubauer, and S. Al Dahouk. 2013. Interlaboratory Comparison of Intact-Cell Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry Results for Identification and Differentiation of Brucella spp. J Clin Microbiol 51:3123-3126.

ChlamydiosisForsbach-Birk, V., C. Foddis, U. Simnacher, M. Wilkat, D. Longbottom, G. Walder, C. Benesch, M. Ganter, K. Sachse, and A. Essig. 2013. Profiling Antibody Responses to Infections by Chlamydia abortus Enables Identification of Potential Virulence Factors and Candidates for Serodiagnosis. PLoS one 8:e80310.Müller, U., K. Sachse, K. Kemmerling, C. Rietz, and H. Sauerwein. 2013. Identification of certain management parctices and health data associated with Chlamydia infection status in German dairy herds. Vet J 197:905-907.

Glanders:Khan, I., S. Ali, M. Gwida, M.C. Elschner, M. Ijaz, A.A. Anjum, and H. Neubauer. 2013. Prevalence of Burkholderia mallei in Equids of Remount Depot, Sargodha, Pakistan. Pakistan J Zool 45:1751-1756.

Paratuberculosis:Elze, J., E. Liebler-Tenorio, M. Ziller, and H. Köhler. 2013. Comparison of prevalence estimation of Mycobacterium avium subsp. paratuberculosis infection by sampling slaughtered cattle with macroscopic lesions vs. systematic sampling. Epidemiol Infect 141:1536-1544.

Kasnitz, N., H. Köhler, M. Weigoldt, G.-F. Gerlach, and P. Möbius. 2013. Stability of genotyping target sequences of Mycobacterium avium subsp. paratuberculosis upon cultivation on different media, in vitro- and in vivo passage, and natural infection. Vet Microbiol 167:573-583.


Zoonoses in Europe

Q-feverMarenzoni, M.L., V. Stefanetti, P. Papa, P. Casagrande Proietti, A. Bietta, M. Coletti, F. Passamonti, and K. Henning. 2013. Is the horse a reservoir or an indicator of Coxiella burnetti infection? Systematic review and biomolecular investigation. Vet Microbiol 167:662-669.

Henning, A.-K., M.H. Groschup, T.C. Mettenleiter, and A. Karger. 2013. Analysis of the bovine plasma proteome by matrix-assisted laser desorption/ionisation time-of-flight tandem mass spectrometry. Vet J [Epub ahead of print; doi:10.1016/j.tvjl.2013.10.029]Georgiev, M., A. Afonso, H. Neubauer, H. Needham, R. Thiéry, A. Rodolakis, H.J. Roest, K.D. Stärk, J.A. Stegeman, P. Vellema, W. van der Hoek, and S.J. More. 2013. Q fever in humans and farm animals in four European countries, 1982 to 2010. Eurosurveillance 18:20407.

TularemiaOtto, P., V. Chaignat, D. Klimpel, R. Diller, F. Melzer, W. Müller, and H. Tomaso. 2013. Serological Investigation of Wild Boars (Sus scrofa) and Red Foxes (Vulpes vulpes) As Indicator Animals for Circulation of Francisella tularensis in Germany. Vector-Borne Zoon Dis [Epub ahead of print; doi.10.1089/vbz.2013.1321]Sting, R., M. Runge, T. Eisenberg, S. Braune, W. Müller, and P. Otto. 2013. Comparison of bacterial culture and polymerase chain reaction (PCR) for the detection of F. tularensis subsp. holarctica in wild animals. Berl Münch Tierärztl Wschr 126:285-290.

Transmissible spongiforme encephalopathies:Priemer, G., A. Balkema-Buschmann, B. Hills, and M.H. Groschup. 2013. Biochemical Characteristics and PrPSc Distribution Pattern in the Brains of Cattle Experimentally Challenged with H-type and L-type Atypical BSE. PLoS one 8:e67599.

Wadsworth, J.D., S. Joiner, J.M. Linehan, A. Balkema-Buschmann, J. Spiropoulos, M.M. Simmons, P.C. Griffiths, M.H. Groschup, J. Hope, S. Brandner, E.A. Asante und J. Collinge 2013. Atypical scrapie prions from sheep and lack of disease in transgenic mice overexpressing human prion protein. Emerg Infect Dis 19: 1731-1739. doi: 10.3201/eid1911.121341.

b) International conference presentations:.........................n.d.

c)National conference presentations:...........................................n.d.

Selected presentations of the FLI (incomplete list):Elschner, M., Neubauer H. – Glanders- a pespective by the OIE-Reference Laboratory, Presentation at the Annual Congress of (IAVMI) Indian Association of Veterinary Microbiologists, Immunologists and Specialists in Infectious Diseases, 13-15 December, 2013, Lucknow, India16th International Symposium of the World Association of Veterinary Laboratory Diagnosticians: WAVLD, 5 -8 June 2013, Berlin (several presentation at different workshops)6. Riemser Diagnostiktage, National Symposium on the diagnostics of animal diseases. 21 -22 November 2013 Greifswald. Contributions including avian influenza, rift valley fever, west Nile fever, Usutu disease, bovine tuberculosis, cow pox, brucellocis, Schmallenberg virus infection


Selected presentations of the FLI (incomplete list):Balkema-Buschmann A., L. Kwasnitschka, K. Fischer, G. Kerth, V. Zeus, B. Ohlendorf and M. H. Groschup : ‘PCR Screening for Viral Nucleic Acid Sequences in Bat Colonies in Germany’. 7th Annual Epizone Meeting, Brüssel Sept 2013Balkema-Buschmann A. and M.H. Groschup: ‘Atypical Scrapie: Intra- and Interspecies Profile’ 11th Annual Conference of Neuroscience Society of Nigeria, Ibadan Nov 2013Balkema-Buschmann A., C. Fast, G. Priemer und M.H. Groschup: ‘Pathogenesis of classical and atypical BSE in cattle’. 11th Annual Conference of Neuroscience Society of Nigeria, Ibadan Nov 2013Harder, T. Vaccines against swine influenza – 5th World Vaccine Congress 18 – 21 March 2013, Hangzou, China

Harder, T. Birds as reservoirs of orthomyxo- paramyxo and flaviviruses. – Nordic-Baltic Seminar on the role of the wildlife as reservoir and /or spread of infectious animal diseases in the coastal areas of countries bordering the Baltic Sea, Nordic Veterinary Contingency Group 2 -3 October 2013 Gdansk, PolenHarder, T. Emerging infectious diseases - 2nd Workshop of the European Network on Viral Vaccine Processes, DECHEMA 10 – 11 November 2013, Frankfurt a.M., GermanyHarder, T. Cost benefit trade-offs in influenza monitoring – Meeting of the national reference laboratories, European Commission, 22 – 24 May 2013, Helsinki, FinnlandHänsel CH, F Melzer, R Diller, HD Zacher, A Henke, H Neubauer, MC Elschner: Establishment Of An Interferon-Gamma-Release-Assay For Glanders Diagnosis In Horses – First Results. 16th Intern Symposium of the World Association of Laboratory Diagnosticians, June 5-8, 2013 Berlin Abtractbook S.221.

Koziy R., M.C. Elschner, F. Melzer , V. Skrypnyk, A. Skrypnyk Evaluation of several Complement Fixation Test Antigens and a RBT antigen for Serological Diagnosis of Glanders 16th Intern Symposium of the World Association of Laboratory Diagnosticians, June 5-8, 2013 Berlin Abstractbook S. 194Moser, I. Spoligotyping Microarray, EU-Referencenlaboratory meeting Madrid 2013

Ulrich, R.G. Rodent monitoring in Germany 2010-2012, EDENext, Annual meeting, Barcelona, 19.03. 2013.

Zoonoses in Europe

d) Other:..............................................................................21

(Provide website address or link to appropriate information)

Manfred Weidmann, Mandy Elschner: Practical Considerations for Collection and Transportation of Hemorrhagic Fever Samples - Chapter 11 IN: Viral Hemorrhagic Fevers Sunit K . Singh and Daniel Ruzek CRC Press 2013 Print ISBN: 978-1-4398-8429-4, eBook ISBN: 978-1-4398-8431-7

Banyard, A.C., D.T.S. Hayman, C.M. Freuling, T. Müller, A.R. Fooks, and N. Johnson. 2013. Bat Rabies. In: Jackson, A.C. (ed.), Rabies - Scientific Basis of the Disease and Its Management, 3rd Edition, Academic Press, ISBN: 978-012396547-9, 704 p., p. 215-267.Barrow, P.A., and U. Methner. 2013. Vaccination against Salmonella Infections in Food Animals: Rationale, Theoretical Basis and Practical Application. In: Barrow, P., and U. Methner (eds.), Salmonella in Domestic Animals, 2 nd Edition, CAB International, ISBN: 978-1-8459-3902-1, 560 p., p. 455-475.

Belaya, V., H. Hansen, and B. Pinior. 2013. Measuring the Costs of Foodborne Diseases: a Review and Classification of the Literature. Schriften der Gesellschaft für Wirtschafts- und Sozialwissenschaften des Landbaues e.V. 48:47-58.

Fast, C. and M.H. Groschup. 2013. Classical and Atypical Scrapie in Sheep and Goats. In: Zou, W.-Q. and P. Gambetti (eds.), Prions and Diseases: Vol. 2, Animals, Humans and the Environment, Springer Science+Business Media, New York, ISBN: 978-1-4614-5338-3_2, 295 p., p. 15-44.Foster, N., and A. Berndt. 2013. Immunity to Salmonella in Farm Animals and Murine Models of Disease. In: Barrow, P., and U. Methner (eds.), Salmonella in Domestic Animals, 2nd Edition, CAB International, ISBN: 978-1-8459-3902-1, 560 p., p. 136-161.

Johnson, N., A.R. Fooks, C. Freuling, T. Müller, H. Ün, O. Aylan, N. Ünal, S. Eskiizmirliler, N. Akkoca, and A. Vos. 2013. The Role of Phylogeography in the Control of Wildlife Rabies in Turkey. In: Introduction to Sequence and Genome Analysis III, iConcept Press Ltd., ISBN: 978-1-922227-09-6, 279 p. URL: http://www.iconceptpress.com/www/site/papers.webView.php?publicationID=BK025-3-1

Shivaprasad, H.L., U. Methner, and P.A. Barrow. 2013. Salmonella Infections in the Domestic Fowl. In: Barrow, P., and U. Methner (eds.), Salmonella in Domestic Animals, 2nd Edition, CAB International, ISBN: 978-1-8459-3902-1, 560 p., p. 162-192.

Wernike, K., B. Hoffmann, and M. Beer. 2013. Schmallenberg virus. In: Roth, J.A., J.A. Richt, I.A. Morozov (eds.), Vaccines and Diagnostics for Transboundary Animal Diseases, Dev Biol, Basel, 135:175-182.

Rabies Bulletin Europe: Rabies Information System of the WHO Collaboration Centre for Rabies Surveillance and Research (4 issues per year) http://www.who-rabies-bulletin.org

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http://www.who-rabies-bulletin.org/

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African horse sickness - Home: OIE - World Organisation … · Web viewEvaluation of several Complement Fixation Test Antigens and a RBT antigen for Serological Diagnosis of Glanders