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food microbiology
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Aeromonas
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Aeromonas spp.
A gramnegative rod, facultative anaerobic, oxidase positive,
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ferments glucose
Stationary psykrofiilisia aeromonadeja (A. salmonicida) and mobile
aeromonadeja mesophilic (A. hydrophila, A. sobria, A. caviae)
DNADNA hybridization: genotypes (1517) versus phenotyyppeja (14)
Opportunistic infections and gastroenteritiksia
= Aeromonas virulence factors of toxins: aerolysiini (haemolysiini)
enterotoxin, proteases, lipases and others.
Pathogeeniset Aeromonas spp .: A. HG 1 hydrophila, A. HG4 caviae, A. veronii
biovar sobria HG8
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Aeromonas spp.
Commonly found in aquatic environments, particularly in fresh water
Can be easily isolated from fish, shellfish, meat and grate
vegetables
Appears in pork, beef, sheep and chickens in the faeces
Grows jääkaappiläpötiloissa (<5 ° C)
Sensitive to low pH, Aw
and CO2
Minimally processed vegetables = a risk foods Aeromonas spp. As regards
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Aeromonas spp. Diseases
The pathogen in humans and animals
Disease formats vary depending on the animal and
aeromaslajista
Blood poisoning A. veronii biotype Sorbs
Gastrointestinal infections A. hydrophila
A. caviae
A. veronii
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Aeromonas spp. growth
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growth area for 342 ºC
optimum 1520 ° C
increases the pH from 4.5 to 10.0
a minimumw
0.91
3.5% NaCl inhibits the growth of
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Aeromonas spp. Toxins
part of the produce heatresistant (30 min at 100 ° C) toxins
foodstuff
toxins produced in food are not to blame
Food poisoning
The symptoms caused by the implantation of aeromonaksien
intestinal cells and the toxin in the intestines return
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The clinical picture
watery diarrhea Heals itself!
abdominal pain, pahonvointia, vomiting and fever
The duration of symptoms in children less than one week and adults under 10
days
parts symptoms persist up to several weeks
1025% ripuleista have bloody diarrhea (reminiscent of shigellosis)
Ulostamiskipua
Fever
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The clinical picture
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Infections Peritoneum
The pericardium
Lung
Ureteral
Wound
The conjunctiva of the eye
Vakavaoireiset requiring hospitalization
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Infective dose
Not applicable
Cause of foodstuffs isolated 00010 1000 000 000
cfu / g
Even large Volunteers feed amounts are not
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caused disease
Not included in yeisvaarallisiin not notifiable infectious diseases
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Participation in humans
Isolate sivulöydöksinä occasionally
diarrhea, stool studies
In temperate zones, 3% carriers
In the tropics and developing countries, the applicants for more than 30%
The environment and isolated from stools positions differ
one another
Environmental strains only part of the are thought to be able to
cause disease
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Participation in food
A. hydrophila commonly found in animal
foods
A. caviae, particularly vegetables
In Europe the most common food isolated is A.
hydrophila
Japan A. caviae
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Epidemics
Has not had outbreaks in Finland
Aeromonakset caused mainly individual
illness
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Minimally processed vegetables
Aeromonas demonstrable per 25 grams, but low levels
In previous studies, the sample containing 6/27
Aeromonas bacteria per 25 grams, but later
(Neyts et al, 2000), only 2/27> 100 g
Aeromonas did not increase during processing.
Competitive flora may inhibit Aeromonas growth as formulated
1.12 log Aeromonas growth of lettuce seven day 7 ° C
(Jacxsens et al, 1999) (inoculated with 10 4 cfu per g)
Wash waters, low levels of Aeromonas (110 per ml)
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Disinfection chlorite
Aeromonas can survive and + 4 and 30 ° C (max 0.5
log reduction 120 min) (0.18 mg per l free chlorine)
(Brandi et al, 1999).
Cfu of E. coli not detectable 10 min and kloriittivedessä
+ 4 ° C to 30
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15% ETOH
Gray = 1 day famished
Darker = 50 days famished
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