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A Novel Approach to Lactate Sensing. Christine Zhang, Stephanie Wu, Joseph Sun, Wern Ong, Toby Li. Objectives. Problem Market Potential Background Requirements Design Verification and Validation Current Status Questions . Problem Statement. - PowerPoint PPT Presentation
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A Novel Approach to Lactate Sensing
Christine Zhang, Stephanie Wu, Joseph Sun, Wern Ong, Toby Li
Problem Market Potential Background Requirements Design Verification and Validation Current Status Questions
Objectives
Fill the market void for a Type II Diabetes prescreening tool
Elevated lactate levels are highly correlated with type 2 diabetes onset
Problem Statement
Chronic illness characterized by high blood sugar
Insulin is a hormone produced by the pancreas to control blood sugar
Type II diabetes is caused by insufficient insulin levels, most often because the body has become resistant to insulin
Diabetes
Death Coronary artery disease Diabetic retinopathy Erection problems Hypertension Infections of the skin, female urinary tract,
and urinary tract Peripheral vascular disease resulting in
gangrene, amputations Stroke
Symptoms
Diabetes causes decreased lactate threshold LT is a point during exhaustive, all-out
exercise at which lactic acid builds up in the blood stream faster than the body can remove it
Diabetes & Lactate
Type II Diabetes 170 million
worldwide, 20.8 million US
300 million by 2025 – WHO prediction
Diabetes is a $15 billion dollar industry
Market Potential
Fasting Blood Glucose Test Fast overnight Go into clinic Take blood glucose
measurement Drink a 75g sugar beverage Take blood glucose
measurement 2 hours later Common Complaints
Nausea Pain Long wait time
Current Testing Methods
Background Information
What is lactate? Byproduct of anaerobic
metabolism - little O2 in environment
Lactate
Saliva and Blood Lactate Levels
Saliva and blood lactate levels show high correlation
Hydrogel Mechanism
pH sensitive hydrogels coupled with lactate oxidase 2-hydroxyethyl methacrylate (HEMA) N,N,dimethylaminoethylmethacrylate
(DMAEMA) Tetraethylene glycol dimethacrylate (TEGDMA)
Crosslinking agent
Hydrogel
Design
According to the CDC and WHO, there is a need for a: Cheap Uniform Reliable Accurate Easy-to-use
…predictor for prediabetics
Performance Criteria
Hygienic Non-invasive Inexpensive Easy to use Accurate Disposable
Design Goals
Directional swelling Thermometer-like
device Physical Change
Colorimetric Assay Color change pH sensitive indicator
Internal Control
Lactate Sensor
Lactate sensing 0.1 mmol resolution – if
digital Range 0.15 – 0.3mmol 0.2mmol indicator level
Performance Metrics
How much Saliva?
Interaction Time?
Exact specification of Hydrogel Preparation
Testing & Verification
Hydrogel filled capillary detects lactate levels in saliva
Directional Swelling/Colorimetric Assay indicates lactate concentrations
Indicate probability of type II diabetes
Solution Overview
Initial Hydrogel Preparation Testing Hydrogel Preparation Specifications Lactate Oxidase Concentration
Current Status
Testing Colorimetric Assay coupled to specific lactate
levels Establishment of Testing Standards/Protocols Testing varying lactate concentrations under
different situations Fabrication
Calibration of Sensor
In the Future…
http://www.bme.vanderbilt.edu/srdesign/2010/group11/
Website
Questions?