A Green Approach- Synthesis of Nanoparticles from Plant ... file10/11/2017 1/20 A Green Approach- Synthesis of Nanoparticles from Plant Extracts Presented by Dr. D. Shalini, AP/ Chemistry

10/11/2017 1/20

A Green Approach- Synthesis of Nanoparticles from

Plant Extracts

Presented by Dr. D. Shalini, AP/ Chemistry

10/11/2017 SNSCE/S&H/Dr.D.Shalini/AP/Chemistry 2/20

Particles having at least one dimension in the range of 1-100 nm

Utilization of a set of principles that reduces or eliminates the use or generation of hazardous substances in the design, manufacture, and application of chemical products


Methods of preparation


Advantages and Disadvantages

High cost

Use of toxic solvents

High energy consumption

Generation of hazardous by-products

Limitations of chemical and physical methods

Advantages of Green synthesis

eco-friendly

Low cost & Can be used for large scale synthesis

Short production time

No need to use high pressure, energy, temperature

and toxic chemicals


Green synthesis of metal nanoparticles

The plant systems are reported as reliable green, eco friendly approach for metal nanoparticle synthesis.

Green synthesis of metal nanoparticles has been an emerging highlight of the research in the field of Nano biotechnology and chemistry Due to growing need for the development of the environmentally benign technologies especially in material science and medicine.


Green synthesis of metal nanoparticles using plant extract

The reduction method using plant extracts is one step, low cost and eco-

friendly, hence considered as the most preferred way for the synthesis of

metal nanoparticles.

Thus, this method may be included in the class of green technology.

Among various nano metals explored so far, nanoparticles of silver, gold,

copper, zinc, palladium, titanium, nickel, indium etc. have been prepared by

using a wide variety of plant extracts.


METHOD - GREEN SYNTHESIS OF METAL NANOPARTICLES



Metal Nanoparticles

Electron micrographs of the iron (A), silver (B), and gold (C) nanoparticles

synthesized in extracts of N. benthamiana at room temperature


Vinca (Madagascar periwinkle) has more than 400 known alkaloids. Some of these are used by the pharmaceutical industry for the treatment of childhood leukemia, Hodgkin’s disease and cancerous tumors. Taken as a daily herbal supplement, it improves the blood supply to the brain, increases oxygen and glucose for the brain to use. There are two classes of active compounds in Vinca: alkaloids and tannins. The major alkaloid is known as vincamine.


Green synthesis of silver nanoparticles

using vinca rosea plant extract

•Main objective of the work is aimed for synthesis of silver nanoparticle using Vinca roseus leaf ,

stem and flower extract.

•The reduction and stabilization capability of the plant extracts of different parts are described.

•The size and structure of the silver can be characterized by varying the plant parts of the Vinca

rosea .

•The biosynthesized nanoparticles are characterized by using UV–VIS spectroscopy, TEM, XRD and

FTIR.

•Catalytic activity of the biosynthesized nanoparticles is established in the degradation of organic

dyes.


Materials and method

Synthesis of silver nanoparticles

Silver nanoparticles stem (S), flower (F) and leaf (L) have been synthesized by

Bhakya et al.[1] method with slight modification. The formation of AgNPs is

indicated by the appearance of dark brown color within 5 min.

Characterization

UV–visible spectra analysis was performed for all samples. The biosynthesized

AgNPs were examined for the presence of bio molecules using UV spectrum .The

crystalline nature of particles was evaluated by using XRD. The studies on size and

shape of silver nanoparticles were performed by transmission electron microscopy


Figure 1 shows the UV–Vis spectra of silver colloids of S,F and L respectively. It is an important method to determine the formation of metal nanoparticles in colloidal solution. It is observed that the maximum absorbance of Ag nanoparticles occurs at 430 nm.


FTIR spectrum was examined to identify the possible biomolecules responsible for capping and efficient stabilization of the biosynthesized Ag nanoparticles, Which can help in further fictionalization with other molecules for various applications in future. The possible mechanism for the reduction of Ag+ to AgNPs is that phenolic OH groups present in hydrolysable tannins can form intermediate complexes with Ag+ ions which consequently undergo oxidation to quinone forms with subsequent reduction of Ag+ to Ag nanoparticles


Photo catalytic Degradation of Dye

Typically 10 mg of methylene blue dye was

added to 1000 mL of double distilled water

used as stock solution.

About 10 mg of biosynthesized silver

nanoparticles was added to 100 mL of

methylene blue dye solution.

A control was also maintained without

addition of silver nanoparticles


While decreasing the concentration of dye shows silver nanoparticles at 22 h of

exposure time .

The percentage of degradation efficiency of silver nanoparticles was calculated as

95.3% at 72 h .

The degradation percentage was increased as increasing the exposure time of dye

silver nanoparticles complex in sunlight .

Methylene blue dye was which diminished and finally it disappeared while

increasing the reaction time, which indicates that the dye had been degraded.


•To evaluate the antibacterial efficiency of synthesized silver nanoparticle against E.coli and staphylococcus. •Free radical scavenging activity determined through DPPH assay. •The synthesized silver nanoparticle from novel plant along with gelatin were used in sustained drug delivery study for diabetics •The AgNPs incorporated with gelatin and drug was characterized using various analyses such as UV-Vis spectroscopy, FTIR and TGA. •The characterized AgNPs and gelatin with drug was taken for Invitro drug release study in mice



CONCLUSION

Different methods (physical, chemical and biological) are available to obtain NPs of various shapes and sizes.

Biological method of NPs is economically and environmentally friendly alternative to chemical and physical approaches.

It provide a new possibility of synthesizing NPs using natural reducing and stabilizing agents.

It has faster synthesis rates, controlled toxicity and well- characterized NPs.

This method is used in various areas such as pharmaceuticals, cosmetics, foods and medical applications


Documents

A Green Approach- Synthesis of Nanoparticles from Plant ... file10/11/2017 1/20 A Green Approach- Synthesis of Nanoparticles from Plant Extracts Presented by Dr. D. Shalini, AP/ Chemistry