Electronic Supplementary Information

Formation of Simple single-tailed Vesicles Mediated by

Lipophilic Solid Surface

Na Du, Xiaoyu Zhu, Ruiying Song, Renjie Zhang, Shue Song and Wanguo Hou*

Key Laboratory of Colloid and Interface Chemistry (Ministry of Education), Shandong

University, Jinan 250199, P.R. China

a b c

d e f

g h i

Figure S1 Negative-staining TEM images of LSB aqueous solutions with (a, d, g) pH 4.0, (b, e, h) pH 6.8, and (c, f, i) pH 9.0 at LSS-mediation time of (a, b, c) 1 day, (d, e, f) 10 days, and (g, h, i) 30 days. Scale bar = 500 nm.

Electronic Supplementary Material (ESI) for Soft Matter.This journal is © The Royal Society of Chemistry 2016

a

b c d

Figure S2 Cryo-TEM images of LSB aqueous solutions with (a) pH 4.0

and (b, c, d) pH 9.0 at a LSS-mediation time of 9 days. Scale

bar = 200 nm.

0 10 20 30 400

10

20

30

40LSS removalwith LSS

A v(a. u

.)

tsm (day)

0 10 20 30 400

10

20

30

40

50with LSS LSS removal

A v(a. u

.)

t (day)0 10 20 30 40

0

10

20

30

40

50 with LSS LSS removal

A v(a. u

.)

t (day)

(a) (b) (c)

Figure S3 Variation of the vesicle peak area for LSB sulutions at (a) pH

4.0, (b) pH 6.8, and (c) pH 9.0 over different LSS-mediation

time and after the removal of LSS.

Figure S4 Negative-staining (a, c, e) TEM images and freeze-fracture (b,

d, f) TEM images of LSB vesicle systems over 20 days after

the LSS removal at (a, b) pH 4.0, (c, d) pH 6.8, and (e, f) pH

9.0.

Figure S5 NS-TEM images of LSB solutions (pH 6.8) with concentrations of

(a) 10 mM, (b) 50 mM, and (c) 100 mM at a LSS-mediation time

of 9 days.

-0.5 0.0 0.5 1.0

40

45

50

55

60

(mN·m

-1)

logC

Figure S6 Semi-logarithmic plots of surface tension (γ) vs. concentration (c)

of purified LSB solution. The cmc of the LSB solution is ~3 mM.

ca b

1200 1000 800 600 400 200 0

O K

LL

O 2

s Ti

3s

TiO2+LSB-LSS

Ti 3

p

N 1s

S 2p

C 1s

Ti 2

s

Ti 2

pO

1s

Binding Energy (eV)

Coun

ts / s

TiO2+LSB

Figure S7 XPS survey spectra for LSB vesicle solution with and

without LSS-mediation. Both of the samples were dried on

titania. Some peaks were indexed with reference [3].

Figure S8 Negative-staining TEM images of LSB vesicle solutions with (a–c)

pH 4.0, (d–f) pH 6.8, and (g–i) pH 9.0 after (a, d, g) thermal

treatment at 80 °C for 2 h, and (b−i) freezing at (b, e, h) −20 °C

and (c, f, i) −196 °C for 2 h and then thawing at room temperature.

1 10 100 10000.00

0.05

0.4

0.6

0.8 (a) 80 C -20 C -196 C

Dh (nm)

f (D h

)

1 10 100 10000.00

0.05

0.10

0.4

0.6 80 C -20 C -196 C

Dh (nm)

(b)

f (D h

)

1 10 100 10000.00

0.05

0.10

0.4

0.6 80 C -20 C -196 C

(c)

Dh (nm)

f (D h

)

Figure S9 Hydrodynamic diameter distributions of LSB vesicle solutions

through thermal treatment, freezing treatment and then thawing at

room temperature at (a) pH 4.0, (b) pH 6.8, and (c) pH 9.0.

Figure S10 Negative-staining TEM images of LSB vesicles under different

concentrations of ethanol: (a, c) 5% (v./v. %) and (b, d) 20%

(v./v. %), at pH 4 (a, b) and pH 9 (c, d).

200 nm

Figure S11 Negative-staining (Left) TEM images and freeze-fracture (Right)

TEM images of vesicles in (a, b) DTAB and (c, d) SDS solutions

at a LSS mediated time of 9 d.

1 10 100 10000.0

0.1

0.2

0.3

1.4

f (D h

)

Diameter (nm)

0d; 7d; 14d; 14d after LSS removal

1 10 100 10000.0

0.1

0.2

0.8

f (D h

)

Diameter (nm)

0d; 7d; 14d; 14d after LSS removal

(a) (b)

Figure S12 Hydrodynamic diameter distributions of (a) DTAB and (b) SDS

vesicle solutions over different LSS-mediation time and after the

removal of LSS.

a b

c d

a b c d

e f g i

Figure S13 Negative-staining TEM images of (a-d) DTAB and (e-i) SDS

vesicle solutions through (a, e) 20 days storage, (b, f) thermal

treatment at 80 °C for 2 h, (c, g) freezing at −20 °C for 2 h and (d,

i) freezing at −196 °C for 2 h and then thawing at room

temperature.

1 10 100 1000 100000.00

0.05

0.10

0.15

0.20

80 C -20 C -196 C

f (D h

)

Diameter (nm) 1 10 100 1000 100000.00

0.05

0.10

0.15

0.20 80 C -20 C -196 C

f (D h

)

Diameter (nm)

(a) (b)

Figure S14 Hydrodynamic diameter distributions of (a) DTAB and (b) SDS

vesicle solutions through thermal treatment, freezing treatment

and then thawing at room temperature.