5 th International Conference on Advance Molecular Bioscience …icambbe.ub.ac.id/wp-content/uploads/2018/09/ICAMBBE-2018.pdf · 2018-09-03 · Dr. Syafarudin, B.Eng., M.Eng. (Deputy

5 th International Conference on Advance Molecular Bioscience and

Biomedical Engineering (ICAMBBE) 2018 Malang | i


Biomedical Engineering (ICAMBBE) 2018 Malang | ii

5th International Conference on Advanced Molecular Bioscience and Biomedical

Engineering (ICAMBBE) 2018

Swiss Belinn Hotel, Malang, East Java, Indonesia

September, 3rd – 4th 2018


Biomedical Engineering (ICAMBBE) 2018 Malang | i

Welcome Speech

Rector of Universitas Brawijaya

On behalf of Universitas Brawijaya, I would like to welcome all of invited speakers and

participants of the 5th International Conference on Advanced Molecular Bioscience and

Biomedical Engineering (ICAMBBE) 2018 from many universities in Indonesia and foreign

countries. Considering the noble aim this event, we are highly appreciating and excited to

hold this event for the five times since 2013.

Universitas Brawijaya, consisting of 15 Faculties and schools of Graduate and Vocational

program, commits to cooperate in scientific aspects in accordance with our mission to be a

World Class University. This scientific event, we wish that this initial effort to integrate the

networking and research would support the exchange of information, research, and

technological advances in the field of university as one approach to support molecular

bioscience and biomedical issues in Indonesia, which is as theme of this conference:

Development of Health and Pharmaceutical Research Competitiveness toward

Sustainability Development Goals (SDGs). Universitas Brawijaya received an award from

the Ministry of Research, Technology and Higher Education, Republic of Indonesia in 2018

as an Institution with the highest Productivity Publication Award in the PTN-BLU (Public

Service University) Category.

Besides that, we have initiative to create harmony in collaboration among Academics,

Business (Industry), and Government to promote our Community, that we called as

ABGC. We proud that Institut Biosains as a part of Universitas Brawijaya could start and

bridging this collaboration. Therefore, it could become a role model for others to achieve

our purpose as a university that applied an ABGC collaboration. Firstly, Institut Biosains

UB collaborated with BioFarma Company, Bandung and now we are in progress to

prepare for mass production of Diagnostic Kit based on autoantibody GAD65 that is able

to predict early stage of type 1 Diabetes Mellitus.

We would like also acknowledge to our keynote speaker, Ministry of Health, National

Institute of Health Research and Development, Republic of Indonesia; Deputy Director of

Industrial Technology Development- Energy & Transportation; as well as other plenary

speakers from Kyushu University; Central Pharmaceutical Research Institute JT. Inc.;

Niigata University, Japan; Suranaree University of Technology, Thailand; also from

Universitas Brawijaya.

We sincerely hope that this activity is beneficial for all of us, especially in an effort to

accelerate the development of education and science in Indonesia. We also hope that this

international conference can improve the quality of research in Indonesia but also make

the relationship between all universities for a better and improving the quality of human

kind.


Biomedical Engineering (ICAMBBE) 2018 Malang | ii

We expect that the international conference will be held continuously every year in

Universitas Brawijaya as our motto to achieve our visions Universitas Brawijaya, build up

noble future.

Malang, September 3th 2018

Sincerely

UB Rector

Prof. Dr. Ir. Nuhfil Hanani, AR. M.S


Biomedical Engineering (ICAMBBE) 2018 Malang | iii

Welcome Speech

Head of 5th International Conference on Advance Molecular Bioscience and Biomedical

Engineering ( ICAMBBE) 2018

Dear participants,

On behalf organizing committee of this event, it’s my great pleasure to welcome to 5th

International Conference on Advanced Molecular Bioscience and Biomedical Engineering

(ICAMBBE) 2018. This conference was held after a great success in, 1st, 2nd, 3rd and 4th

ICAMBBE last years. This year, the conference will bring a new theme about Development

of Health and Pharmaceutical Research Competitiveness toward Sustainability

Development Goals (SDGs). Hopefully it will cover all related knowledge and bring the

new insight for a better quality of life. Once again, the conference will bring together

leading researchers, engineers and scientists in the domain of interest from around the

world; therefore, it became a new step to realizing a good collaboration from all aspects.

The objectives of this conference is to share their experience, new ideas and research result

that give positive contributions for the better of our life in the future. Based on our theme,

we divided this conference into five scopes could cover all aspects in life sciences.

On this occasion, we profusely thank to:

dr. Siswanto, MPH, DTM (National Institute of Health Research and Development,

Ministry of Health, Republic of Indonesia);

Dr. Syafarudin, B.Eng., M.Eng. (Deputy Director of Industrial Technology Development –

Energy & Transportation);

Prof. Ken-Ichirou Morohashi, Ph.D (Molecular Biology, School of Medical Science, Kyushu

University, Japan);

Tomohiko Sasase, Ph.D (Lead Guest Editor in International Journal of Endocrinology,

Special Issue on Animal Models of Diabetes and Related Metabolic Disease; Central

Pharmaceutical Research Institute, JT Inc. Japan);

Takeshi Ohta, Ph. D (Central Pharmaceutical Research Institute, JT Inc, Japan);

Assoc. Prof. Dr. Mariena Ketudat- Cairns, Ph.D (School of Biotechnology Institute of

Agricultural Technology, Suranaree University of Technology, Thailand);

Assoc. Prof. Hideaki Yamashiro, Ph.D (Laboratory of Animal Reproduction, Faculty of

Agriculture, Niigata University, Japan).

In this occasion, 56 presenters in oral, 9 poster will be present their research on this

conference provide many opportunities for discussion. We have many researcher and

lecturer that participate in this Conference from many universities of several countries,

such as France, Netherlands, Japan, India, Taiwan and Indonesia, the most of participant is

scholar student.

We would like to inform that we had collaboration with reputable publisher are Acta

Informatica Medica published by the Academy of Medical Sciences of


Biomedical Engineering (ICAMBBE) 2018 Malang | iv

Scopus and Pubmed Indexing and Journal of Physics: IOP Conference Series of Scopus

Indexed.

Also thank for all participant from many universities and Research centers, such as

University of Poitiers, Poitiers, France; University of Groningen, The Netherlands; Tokyo

University of Agriculture, Japan; Charotar University of Science and Technology,

Changa,Gujarat,India; National Central University, Taiwan; Gadjah Mada University;

Universitas Indonesia; Universitas Airlangga; Bogor Agricultural University; Universitas

Surabaya; Malang State University; Universitas Islam Malang; Universitas Sebelas Maret;

Wijaya Kusuma Surabaya University; State University of Surabaya; Maranatha Christian

University, Bandung; Aretha Medika Utama, Bandung; Universitas Ahmad Dahlan,

Yogyakarta; Christian University of Indonesia; Semarang University; Universitas

Muhammadiyah Semarang; Sultan Agung Islamic University, Semarang;Patimura

University, Ambon; Universitas Khairun Ternate; Universitas Syiah Kuala, Aceh;

Universitas Swadaya Gunung Jati, Cirebon; Nusa Cendana University, Kupang, NTT;

State University of Manado (UNIMA).

Thank you for all participant exhibitions and our sponsorship such as PT. Sciencewerke,

Bank Mandiri, CV. Gamma Scientific and CV. Biotek Prima Indoplus for their support that

have made this event.

We also appreciate to all committee for their hard working to make this conference being

scientifically rewarding experience for us.

We wish that 5th ICAMBBE could give significant contribution towards the science

acceleration. We hope also that this conference can improve the quality of research in

Indonesia and promote the quality of education in Indonesia. We hope that the

international conference will be held continuously every year in Universitas Brawijaya.

Best regards,

Prof. Fatchiyah, M.Kes., Ph.D¬¬


Biomedical Engineering (ICAMBBE) 2018 Malang | v

Table of Content

Welcome Speech from Rector of Universitas Brawijaya .......................................................... ............. i Welcome Speech Head of 5th International Conference on Advance Molecular Bioscience and Biomedical Engineering ( ICAMBBE) 2018 ................................................................................... iii Table of Content……………………………………………………………………………. ........... v Scientific Program

5th International Conference on Advance Molecular Bioscience and Biomedical

Engineering (ICAMBBE) Institut Biosains, Universitas Brawijaya .................................... 01

Schedule of Parallel Seminar .................................................................................................... 03

Keynote Speaker

Development of Health and Pharmaceutical Research Competitiveness toward SDGs. 13

The role of Ministry of Research, Technology, and Higher Education (RISTEKDIKTI) in

Coordinating and Directing the R & D on Health Products and Drugs for Utilization .. 15

Plenary Lecture

PL-1 Recombinant Protein Production for Stem Cell Uses ............................................. 16

PL-2 Orchestration of Housekeeping and Cell-Specific Metabolisms in Steroidogenic

Cells by a Transcription Factor, Ad4BP/SF-1 ........................................................... 17

PL-3 Effect of Radiation Exposure on Reproductive Function of Domestic and

Wild Animals after the Fukushima Nuclear Power Plant Accident .................... 18

PL-4 Pathophysiological Analysis of Diabetic Peripheral Neuropathy in

SDT Fatty Rat, a New Animal Model of Obese Type 2 Diabetes .......................... 19

PL-5 Pharmacological Responses to Anti-Diabetic Drugs in Female SDT Fatty Rats,

A New Obese Type 2 Diabetic Rat ............................................................................ 20

PL-06 Development of Recombinant Protein Based Biomarker for

Autoimmune Diseases ................................................................................................ 21

Oral Presentation

MS - 01 Isolation, Characterization Cancer Cells from Breast Cancer Patients ........... 22

MS – 02 Effectivty Of Betel Leaves (Piper Betle L.) And Breadfruit Leaves

(Artocalpus Atilis) In Reducing Uric Acid Levels In Hyperuricemic Male

White Rats (Rattus Norvegicus) ........................................................................... 23

MS – 03 EFFECTIVITY OF TOMATO (Solanum lycopersicum) AND ZINC

COMBINATION TO SPERMS OF MALE WHITE RATS (Rattus norvegicus)

EXPOSED TO MONOSODIUM GLUTAMATE ................................................. 24

MS – 04 The Level Short Chain Fatty Acids and HSP 70 in Colorectal Cancer and

Non Colorectal Cancer ........................................................................................... 25

MS – 05 Antimicrob activity of Whey Mare’s Milk Against Salmonella enteritidis .... 26

MS – 06 FAS and FASL Polymorphism and Susceptibility to Hepatitis B

Virus Infection in Javanese Individuals ............................................................... 27


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MS – 07 The Comparison on Antihyperglycemic Activity Between Gedong Gincu

Mango Leaf (Mangifera Indica Var. Gedong Gincu) And Metformin in

Stretozotocin-Induced Diabetic Rats .................................................................... 28

MS – 08 The Potential Role of Betatrophin in Hepatocellular Carcinoma and

Metabolic Syndrome ............................................................................................... 29

MS – 09 The Inhibition of Vasa Vasorum Angiogenesis by Darapladib in

Dyslipidemic Conditions ....................................................................................... 30

MS – 10 The Effects of Specific Inhibitor Lp-PLA2 on Vasa Vasorum Angiogenesis

Through Inhibition Vascular Endothelial Growth Factor Expression:

Study In Vivo Using Type 2 Diabetes Mellitus ................................................... 31

MS – 11 Albumin and Enzyme Profile of Dwarf Snakehead, Channa gachua were

Caught from the River in East Java ..................................................................... 32

MS – 12 Protein Profile And Hemagglutination Activity of Pilli, an Adhesion Factor

Causing Typhoid Fever by Salmonella typhi...................................................... 33

MS – 13 The Comparison on Antihyperglicemic Effectiveness Comparison Of

Ethanol Extract Of Soursop Leaf (Annona Muricata L.) Againts Acarbose in

Streptozotocin-Induced Diabetic White Rats ...................................................... 34

MS – 14 Cutaneous Wound Healing Activity of Herbal Ointment Containing the Leaf

Extract of Acalypha indica L. on mice (Mus musculus) .................................... 35

MS – 15 Terbufos and Fenthion Induce DNA damage and Active Homologous

Recombination Repair pathway in vitro and in vivo ........................................ 36

MS – 16 Diabetes Dance of Persadia 1 Effect on Blood IL-6 Level .................................. 37

MS – 17 Effect of Torbangun (Coleus amboinicus Lour) on Blood Pressure in

Women with Hypercholesterolemia ................................................................... 38

MS – 18 Improved Hypoglycemic Effect of Anthocyanin Extract Combination

from Red Rice And Black Soybean ...................................................................... 39

MS – 19 Hypoglycemic Mechanism of High Resistant Starch Sago and Red BBBean

Flour- Based Analog Rice on Diabetic Rats : a Nutrigenomics Study ............. 40

MS – 20 Potential Polysaccharide Gel from Durio Zibertinus var.Raja Galuh Rind

Extract Towards Klebsiella Pneumoniae Bacteria .............................................. 41

MS – 21 Effects of IGF1-Induced Wharton Jelly Mesenchymal Stem Cells (WJMSCs)

Toward Expression of Proinflammatory Cytokine in Osteoarthritis Model .. 42

MS - 22 Progress Report on the Rapid Test Kit Development for Early Detection of

Systemic Lupus Erythematosus in Indonesia ..................................................... 43

MS – 23 Protein Profiling of Coloring Rice (Oryza sativa L.) using SDS-PAGE and

Experiontm260 Analysis ........................................................................................ 44

MS – 24 Comparison of Sport Massage And Combination of Cold Water

Immersion With Sport Massage on Decrease of Blood Lactic Acid Level ...... 45

MS – 25 The Effect of Methanol Extract of Sansevieria Trifasciata Leaves on

Pancreatic Beta-Cells Function T1DM and T2DM Rats ..................................... 46

MS – 26 The Morphological Endogenous Development Of Eimeria Necatrix At

Primary And Secondary Infected Chickens And Its

Histopathological Effect ......................................................................................... 47


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MS – 27 The Aquatic Environmental Quality of Koi Fish (Cyprinus carpio)

Pond Infected by Myxobolus sp. Based on the Biological Status of the

Phytoplankton ......................................................................................................... 48

MS – 28 The Efficacy of Goat Milk Yogurt for Hypercholesterolemic rats

(Rattus norvegicus) models ................................................................................... 49

MS – 29 Chondrocyte Pericellular Matrix Strain Fields Of Articular Cartilage

Surface In Hyperglicemia Model Of Rat: Cellular Morphological Study ....... 50

MS – 30 Anti-inflammatory Effect of Ethyl Acetate Fraction of Galing Plant

Extract (Cayratia trifolia) on Male Wistar Rats Induced by Carrageenan ...... 51

MS – 31 Activity of Combination Persea americana Mill and Orthosiphon

aristatus (Blume) Miq. Benth Extract in dissolving Calcium Oxalate

by In Vitro ................................................................................................................ 52

MG – 01 Changes in Endometrial αv β3 Integrin Expression of Macaca nemestrina

after Administration of Gonadotropin dose of 30-70 IU/day in Controlled

Ovarian Hyperstimulation Procedure ................................................................. 53

MG – 02 Development of a Functional Assay to Study The Impact of CHD7 Missense

Mutations in CHARGE Syndrome ....................................................................... 54

MG – 03 Prospects and Problems the Amplification of Dna Metagenomic From

Clove Plant Collected from Plantation Community in Ternate Island .......... 55

MG – 04 Phylogenetics Relationship of the Genus Cyrtodactylus (Squamata;

Gekkonidae) From Java and Sumatra Based on ND4 Gene ............................. 56

BB– 01 Topical Gel Formulation of Sponge Extract Nanoemulsion with Various

Concentration of CMCNa ...................................................................................... 57

BB– 02 The correlation of Knowledge in Preganant Women and Huband Support

with Antenatal Care (ANC) Compliance at Puskesmas Kersana Brebes ....... 58

BB– 03 Google Trend Analysis on Realization Scope of Pekan Imunisasi

Nasional 2016 ........................................................................................................... 59

BB– 04 Decomposition Rate of Many Organic Waste Using Innovative Biopore ...... 60

BB– 05 A Biomass (Champo Leaf) Astable Multivibrator memory circuit ................. 61

PT – 01 The Effects of Anti-diabetic Metals (V, Mo, W, and Cr) on Cellular

Metabolism of Insulin-Sensitive Cells ................................................................. 62

PT – 02 Conserved Amino Acid Residues of LexA Repressor of ESKAPE

Pathogens and its Potential as a Drug Target ..................................................... 63

PT – 03 Cryoprotectant Combination Ethylene Glycol and Propanediol on Mice

Blastocyst Viability Post Vitrification ................................................................... 64

PT – 04 Okra Infusion Water Improving SOD and CRP Level on Diabetic

Induced Rats ............................................................................................................ 65

PT – 05 Visual, Organoleptic and Physical Detection as Qualitative Indicators

of Formalin-containing Food: Experimental Study on Skipjack Fish Meat

(Katsuwonis pelamis) ............................................................................................. 66

MVS – 01 Reproductive Characters of Senduro Goat at District of Lumajang

East Java .................................................................................................................... 67

MVS – 02 Oviduct Specific Glycoprotein Expresion in Goats (Capra aegagrus)

Testes and Ovariums .............................................................................................. 68


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MVS – 03 REPRODUCTIVE PERFORMANCE dan FERTILITY INDEX SWAMP

BUFFALO (Bubalus bubalis) IN NGAWI REGENCY ....................................... 69

MVS – 04 Effect of Supplementation of Either Powdered or Encapsulated Probiotic

on Carcass Percentage, Giblets and Small Intestinal Morphometric

of Local Duck ........................................................................................................... 70

MVS – 05 IN VITRO FERTILIZATION OF CRYOPRESERVED GOAT OOCYTES

IN DIFFERENT CRYOPROTECTANTS ............................................................. 71

MVS – 06 The Activity of Casein Derived from Goat Milk Yogurt as an

Antioxidant on Histophatology of Rat’s Liver Exposured by 2,3,7,8-

Tetrachlorodibenzo-p-Dioxin (TCDD) ................................................................. 72

MVS – 07 The Conception Rate Of Artificial Insemination By Using Sexing Frozen

Semen On Ongole Crossbred Cows ..................................................................... 73

MVS – 08 Individual Mutations in Indonesian Local Ettawah Goats Based on

The GDF9 Gene ....................................................................................................... 74

MVS – 09 Malondialdehyde (MDA) Level and Protein Profile of Serum After

Calving Towards The Provision of Selenium-Vitamin ETM on Dairy

Cow Frisien Holstein (FH) ..................................................................................... 75

MVS – 10 The Profile Of Red Blood Cells In Sumba Ongole Cattle Is Maintained

In Dryland Areas ..................................................................................................... 76

Poster Presentation

P – 01 Pathophysiological Changes in Salt Loaded Female Spontaneously

Diabetic Torii (SDT) Fatty Rats ............................................................................. 77

P – 02 Interaction of Acetaminophen and Caffeine Towards Cyclooxygenase-2

(COX-2) in Inhibition of Prostaglandin (PGH) Synthesis ................................. 78

P – 03 Effect of Combination Core Stability Exercise and Contract Relax

Exercise On Hamstring Flexibility ........................................................................ 80

P-04 Direct Effect Short Wave Diathermy Therapy and Exercise with

Quadriceps Bench Against Knee Osteoarthritis Patients with Functional

Capabilities ............................................................................................................... 81

P-05 Pathophysiological Evaluation of The Liver in Western Diets Fed

Insulin Resistant Zucker Fatty Rats ...................................................................... 82

P – 06 The Immunogenicity Of Polyclonal Antibody From Induced Vibrio

Parahaemolyticus Membrane By Elisa Method .................................................. 83

P – 07 Isolation Of Lactic Acid Bacteria From Ruments Of Cattle As A Starter

In The Manufacture Of Silage ............................................................................... 84

P – 08 The Applification Of Barcode Dna Rbcl Gen For Identification Of

Medicinal Plants: Red Jabon And Gofasa ............................................................ 85

P – 09 Synthesis, Docking Molecule Study and Antibacterial Activity of

N’-(4-Fluorobenzylidene) -4-hydroxybenzohydrazide, and

N’-(3-Bromobenzylidene) -4-hydroxybenzohydrazide ..................................... 87


Biomedical Engineering (ICAMBBE) 2018 Malang | ix

COMMITTEE 5th International Conference of Advance Molekular Bioscience &

Biomedical Engineering (5th ICAMBBE 2018) September, 3rd-4th 2018 ........................... 88

Accessibility ................................................................................................................................ 89

Site Map General Facilities of Swiss Bell Inn Hotel .............................................................. 90


Biomedical Engineering (ICAMBBE) 2018 Malang | 1

SCIENTIFIC PROGRAM

5th International Conference on Advance Molecular Bioscience and Biomedical

Engineering (ICAMBBE)

Institut Biosains, Universitas Brawijaya

Monday, September 3rd 2018

Time Topic Speaker

07.30-08.00 Registration

08.00-08.45 Opening Ceremony:

National anthem (Indonesia Raya)

Seminar Report by Head of Organizing Committee ICAMBBE 2018

Opening remarks by Rector Universitas Brawijaya

Photo Session

08.45-09.00 Coffee Break 1

Keynote Speech (Moderator: Dr. Ir. Gatot Ciptadi, DESS)

09.00-10.00 dr. Siswanto, MPH, DTM. (Director General of National Institute of

Health Research and Development, Ministry of Health, Republic of

Indonesia)

10.00-11.00 Dr. Syafarudin, B.Eng., M.Eng. (Deputy Director of Industrial

Technology Development – Energy & Transportation)

Plenary Lecture I (Moderator: Dr. Ir. Anik Martinah Hariati, M.Sc)

11.00-11.30 Recombinant Protein

Production for Stem

Cell Use

Assoc. Prof. Dr. Mariena Ketudat-Cairns, Ph.D

(School of Biotechnology, Suranaree University of

Technology, Thailand)

11.30-12.00 Orchestration of

Housekeeping and

Cell-specific

Metabolism In

Steroidogenic Cells by

a Transcription Factor

Ad4BP/5F-1

Prof. Ken-Ichirou Morohashi, Ph.D (Molecular

Biology, Medical Science, Kyushu University, Japan)

12.00-12.30 Discussion

12.30-13.30 Lunch & Break

13.30-14.00 Poster Presentation

Parallel Oral Presentation

14.00-16.50 Parallel

Presentation 1

Parallel

Presentation 1

Coffee Break 2

Parallel

Presentation 2

Parallel

Presentation 2



Tuesday, September 4th 2018

Time Topic Speaker

08.00-08.30 Registration

08.30-09.00 Biosains Product Profile : Development Of Recombinant Protein Based

Biomarker For Autoimmune Diseases (Prof. Dr. drh. Aulanni’am, DES)


Plenary Lecture II (Moderator: Nia Kurniawan, S.Si, MP., D.Sc.)

09.15-09.45

Effect of radiation

exposure on

reproductive function

of domestic and wild

animals after the

Fukushima Nuclear

Power Plant Accident

Asc. Prof. Hideaki Yamashiro, Ph.D

(Nigata University)

09.45-10.15 Pathophysiological

analysis of diabetic

peripheral neuropathy

in SDT fatty rat, a

new animal model of

obese type 2 diabetes

Tomohiko Sasase, Ph.D (Central Pharmaceutical Research Institute, JT. Inc, Japan)

10.15-10.45 Pharmacological

responses to anti-

diabetic drugs in

female SDT fatty rats,

a new obese type 2

diabetic rat

Takeshi Ohta, Ph.D (Central Pharmaceutical Research Institute, JT. Inc, Japan)

10.45-11.15 Presentation of Sciencewerke

11.15-11.45 Discussion

11.45-12.45 Lunch & Break

12.45-13.00 Exhibition Session

Parallel Oral Presentation

13.00-14.50

Parallel

Presentation 1

Parallel Presentation 1

Parallel

Presentation 2

Parallel Presentation 2


15.15-16.00 Closing Ceremony, Announcement of the Best Presenter



Schedule of Parallel Seminar

Oral Presentation Session 1

Date : Monday, 3rd September 2018

Theme : Medical Science (MS) & Biochemistry and Bioengineering (BB)

Room : Arjuna 2

Time Keeper : 1. Rista Nikmatu Rohmah, M.Si

2. Akbar Farid H, S.Si

ID Presenter Title Schedule

MS-01 Wahyu Widowati, Yusuf

H, Dian R.L, Diana K. J,

Teresa L.W, Rizal R,

Fajar S.P, Annisa A,

Annisa A, Zakiyatul K

and Ahmad F.

Isolation, Characterization Cancer Cells

from Breast Cancer Patients

14.00-14.10

MS-02 Firman Faizal, Donny

Nauphar, Tiar M.

Pratamawati and Rama

S. Brajawikalpa

Effectivty of Betel Leaves (Piper Betle L.)

and Breadfruit Leaves (Artocalpus Atilis)

in Reducing Uric Acid Levels in

Hyperuricemic Male White Rats (Rattus

Norvegicus)

14.10-14.20

MS-03 Ignatius H. Wirandoko,

Cici Apriyani and

Dadan Ramadhan

Effectivity Of Tomato (Solanum

Lycopersicum) And Zinc Combination

To Sperms Of Male White Rats (Rattus

Norvegicus) Exposed To Monosodium

Glutamate

14.20-14.30

BB-01 Aida Humaira, Nada H

and Yusuf F. Achmad

Topical Gel Formulation of Sponge

Extract Nanoemulsion with Various

Concentration of CMC-Na

14.30-14.40

BB-02 Azwin Abdul J., Ricardi

W. Alibsjah, Donny

Nauphar, Tiar M.

Pratmawati

The correlation of Knowledge in

Preganant Women and Huband Support

with Antenatal Care (ANC) Compliance

at Puskesmas Kersana Brebes

14.40-14.50

BB-03 Risnandya Primanagara Google Trend Analysis on Realization

Scope of Pekan Imunisasi Nasional 2016

14.50-15.00

BB-04 Winarsih Decomposition Rate Of Many Organic

Waste Using Innovative Biopore

15.00-15.10

BB-05 Gaurang Patel, S.P Kosta

and Jitendra P. Chaudari

A Biomass (Champo Leaf) Astable

Multivibrator memory circuit

15.10-15.20





Theme : Medical Science (MS)

Room : Arjuna 3

Time keeper : 1. Lidwina Faraline Tripsila, M.Si

2. Nur Indah Ratnasari, S.Si


MS-04 Fauzi Yusuf, Siti

Adewiah and

Fatchiyah

The Level Short Chain Fatty Acids and HSP

70 in Colorectal Cancer and Non Colorectal

Cancer Patients.

14.00-14.10

MS-05 Annytha Detha, Agus

Saputra and Antonius

Ola

Antimicrob Activity of Whey Mare’s Milk

Againts Salmonella enteritidis

14.10-14.20

MS-06 Dyah A. Agustin and

Afiono A. Prasetyo

FAS and FASL Polymorphism and

Susceptibility to Hepatitis B Virus Infection

in Javanese Individuals

14.20-14.30

MS-07 Qurotul Aqyun,

Ahmad F.M.Z. Zein

and Vivi

Meidianawaty

The Comparison on Antihyperglycemic

Activity between Gedong Gincu Mango Leaf

(Mangifera Indica var. Gedong Gincu) and

Metformin in Streptozotocin-induced

Diabetic Rats

14.30-14.40

MS-08 Hendra Susanto,

Aulanni’am A, C.H

Wang, Dyah K.

Wuragil, and A.Y

Handaya, M.P Pertiwi

and S.A Rufiatin Nisa

The Potential Role of Belatrophin in

Hepatocellular Carcinoma and Metabolic

Syndrome

14.40-14.50

MS-09 Teuku Heriansyah,

Fitria N. Aini,

Nafisatuzzamrudah,

Rozah F.P, Maya D.W

and Titin A. Wihastuti

The Inhibition of Vasa Vasorum

Angiogenesis by Darapladib in

Dyslipidemic Conditions

14.50-15.00

MS-10 Titin A. Wihastuti,

Nafisatuzzamrudah,

Fitria N. Aini, Kenty

W. Anita, Sari

Kushardianti, Sandra

P.W. Aswuri and

Teuku Heriansyah

The Effects of Specific Inhibitor Lp-PLA2 on

Vasa Vasorum Angiogenesis Through

Inhibition Vascular Endothelial Growth

Factor Expression: Study In Vivo Using

Type 2 Diabetes Mellitus

15.00-15.10

MS-11 Anik M. Hariati, A.

Yuniarti, D.G.R

Wiadnya and W.E

Kusuma

Albumin and Enzyme Profile of Dwarf

Snakehead, Channa gachua were Caught

from the River in East Java

15.10-15.20






Room : Arjuna 2

Time Keeper : 1. drh. Fitria Novitasari

2. Sanudi, S.Pi


MS-12 Sri Darmawati,

Stalis N. Ethica and

Sri S. Dewi

Protein Profile And Hemagglutination

Activity of Pilli, an Adhesion Factor Causing

Typhoid Fever by Salmonella typhi

15.20-15.30

MS-13 Ruri R. Setiadi,

Ahmad F.M.Z Zein

and Donny

Nauphar

The Comparison on Antihyperglycemic Effect

between Ethanol Extract of Soursop Leaf

(Annona Muricata L.) Againts Acarbose in

Streptozotocin-induced Diabetic White Rats

15.30-15.40

MS-14 Meity Laut, Nemay

A. Ndaong and

Trie Utami

Cutaneous Wound Healing Activity of Herbal

Ointment Containing the Leaf Extract of

Acalypha indica L. on mice (Mus Muculus)

15.40-15.50

MS-15 Eva A. Wahyuni,

Chiou H.Y and Ssu-

Ching C

Terbufos and Fenthion Induce DNA damage

and Active Homologous Recombination

Repair pathway in vitro and in vivo

15.50-16.00

MS-16 Wahyu N. Pratiwi,

Bambang P and

Abdurachman

Diabetes Dance of

Persadia 1 Effect on Blood IL-6 Level

16.00-16.10

MS-17 Trini Suryowati

and Moskwadina

Gultom

Effect of Torbangun (Coleus amboinicus Lour)

on Blood Pressure in Women with

Hypercholesterolemia

16.10-16.20

MS-18 Wahidah M.

Rahayu, Mary

Astuti and

Yustinus Marsono

Improved Hypoglycaemic Effect of

Anthocyanin Extract Combination from Red

Rice and Black Soybean

16.20-16.30

MS-19 Sri Budi

Wahjuningsih and

Haslina

Hypocholesterolamic Mechanism of High

Resistant Starch Sago and Red Bean Flour-

Based Analog Rice on Diabetic Rats: A

Nutrigenomics Study

16.30-16.40

MS-20 Amanah A, Tiar M.

Pratamawati,

Mutiara Taslimah

and Risnandya P

Potential Polysaccharide Gel from Durio

Zibertinus Var.Raja Galuh Rind Extract

towards Klebsiella Pneumoniae Bacteria

16.30-16.50

MG-04 Muhammad Ali

Fauzi, Nia

Kurniawan

Phylogenetics Relationship of the Genus

Cyrtodactylus (Squamata; Gekkonidae) from

Java and Sumatra Based on ND4 Gene

16.50- 17.00





Theme : Molecular Genetics (MG) & Medical Science (MS)

Room : Arjuna 3

Time Keeper : 1. Yulianto M. Nugroho, S.Si

2. Syahriar Andinata, S.Pt


MG-01 Nurhuda, Rachman

I.A, Muharram R,

Siregar N.C,

Mujihartini N,

Kusmardia and

Pradhita A

Changes in Endometrial αv β3 Integrin

Expression of Macaca nemestrina after

Administration of Gonadotropin dose of 30-70

IU/day in Controlled Ovarian

Hyperstimulation Procedure

15.20-15.30

MG-02 Gara S. Brajadenta,

Frederic B, Brigitte

G.D, Alain K and

Vincent T

Development of a Functional Assay to Study

The Impact of CHD7 Missense Mutations in

CHARGE Syndrome

15.30-15.40

MG-03 Nurhasanah,

Sundari and

Nurmaya

Papuangan

Prospects And Problems Of Amplification Of

DNA Metagenomic From Clove Plant

Collected From Plantation Community In

Ternate

15.40-15.50

MS-21 Ni Luh W.E. Yanti,

Ervi Afifah, Wahyu

Widowati,

Rismawati L. Q,

Fajar S. P,

Tjandrawati M,

Ferry Sandra, Rizal

Rizal, Annisa

Amalia, Zakiyatul

K, Indra B and

Harry Murti

Effects of IGF1-Induced Wharton Jelly

Mesenchymal Stem Cells (WJMSCs) Toward

Expression of Proinflammatory Cytokine in

Osteoarthritis Model

15.50-16.00

MS-22 Kusworini

Handono, Krist N.

Benita, Fitria U.

Habibah and Elvira

Sari

Progress Report on The Rapid Test Kit

Development for Early Detection of Systemic

Lupus Erythematosus in Indonesia

16.00-16.10

MS-23 Dewi R. T. Sari,

James R. Ketudat

Cairns, Anna Safitri

and Fatchiyah

Fatchiyah

Protein Profiling of Coloring Rice (Oryza

Sativa l.) Using SDS-PAGE and Experion TM-

260 analysis

16.10-16.20

MS-24 Nurul F.

Romadhona, Gadis

M. Sari and

Dwikora N. Utomo

Comparison of Sport Massage And

Combination of Cold Water Immersion With

Sport Massage on Decrease of Blood Lactic

Acid Level

16.20-16.30

MS-25 N.Qomariyah,

M.M.Calon, J.K

Kruit, J.E. Bruggink

The Effect of Methanol Extract of Sansevieria

Trifasciata Leaves on Pancreatic Beta-Cells

Function T1DM and T2DM Rats

16.30-16.40



and G. van Dijk

MS-31 Rini Madyastuti,

Ietje Wientarsih,

Setyo Widodo, Erni

H Purwaningsih,

Eva Harlina

Activity of CombinationPersea Americana

Mill Leaves Extract and Orthosiphon aristatus

(Blume) Miq. Benth Extract in Dissolving

Calcium Oxalate by In Vitro

16.40- 16.50




Date : Tuesday, 4th September 2018

Theme : Pharmacological & Toxicity (PT)

Room : Arjuna 2

Time Keeper : 1. Anita Herawati, M.Si

2. Dessy Wulandari Eka Putri, S.Si


PT-01 Anna Safitri, Aviva

Levina and Peter A.

Lay

The Effects of Anti-diabetic Metals (V, Mo, W

and Cr) on Cellular Metabolism of Insulin-

Sensitive Cells

13.00-13.10

PT-02 Rio Risandiansyah,

Reza Hakim and

Yoni R. Bintari

Conserved Amino Acid Residues of LexA

Repressor of ESKAPE Pathogens and its

Potential as a Drug Target

13.10-13.20

PT-03 Adinda Kresna,

Widjiati and Tita

Damayanti

Ctyoprotectant Combination Ethylene Glycol

and Propandiol on Mice Blastocyst Viability

Post Vitrification

13.20-13.30

PT-04 Nurina Tyagita,

Kurnia P. Utami,

Fitrian H.

Zulkarnain and

Azizah H. Safitri

Okra Infusion Water Improving SOD and CRP

Level on Diabetic Induced Rats

13.30-13.40

PT-05 Johana Z. Wantania

and Alfonds A.

Maramis

Visual, Organoleptic and Physical Detection as

Qualitative Indicators of Formalin-containing

Food: Experimental Study on Skipjack Fish

Meat (Katsuwonis pelamis)

13.40-13.50






Room : Arjuna 3

Time Keeper : 1. Perdana Finawati, S.Si

2. Arifudin, S.TP


MS-26 Muchammad

Yunus

The Morphological Endogenous

Development of Eimeria necatrix at Primary

and Secondary Infected Chickens and its

Histopathological Effect

13.00-13.10

MS-27 Uun Yanuhar, Nico

R. Caesar, Feri

Setiawan,

Muhammad Musa,

Dyah K. Wuragil

The Aquatic Environmental Quality of Koi

Fish (Cyprinus carpio) Pond Infected by

Myxobolus sp. Based on the Biological Status

of the Phytoplankton

13.10-13.20

MS-28 Dyah K. Wuragil,

Masdiana C.

Padaga,

Aulanni’am

Aulanni’am and

Chanif Mahdi

The Efficacy of Goat Milk Yogurt for

Hypercholesterolemic rats (Rattus

norvegicus) models

13.20-13.30

MS-29 Ibrahim Njoto,

Ayly Soekanto,

Ernawati,

Abdurrachman,

Handono K and

Kusworini , D.W.

Soeatmadji,

Fatchiyah

Fatchiyah

Chondrocyte Pericellular Matrix Strain Fields

Of Articular Cartilage Surface In

Hyperglicemia Model Of Rat: Cellular

Morphological Study

13.30-13.40

MS-30 Djoko Santoso, I

Ketut Sudiana,

Anny S.R and

Muchammad

Yunus

Anti-inflammatory Effect of Ethyl Acetate

Fraction of Galing Plant Extract (Cayratia

trifolia) on Male Wistar Rats Induced by

Carrageenan

13.40-13.50






Room : Arjuna 2

Time Keeper : 1. Lidwina Faraline Tripsila, M.Si

2. Sanudi, S.Pi


MVS-1 Gatot Ciptadi, M.N.

Ihsan, Agus

Budiarto M.

Mudawamah and

Arieq Naufal

Reproductive Characters of Senduro Goat at

District of Lumajang East Java

14.00-14.10

MVS-2 Herlina Pratiwi,

Aulia Firmawati,

Herawati and

Nurul Isnaini

Oviduct Specific Glycoprotein Expresion in Goats

(Capra aegagrus) Testes and Ovarium.

14.10-14.20

MVS-3 Agus Budiarto,

Gatot Ciptadi and

L. Hakim

Reproductive performance dan fertility index

Swamp buffalo (bubalus bubalis) in ngawi regency

14.20-14.30

MVS-4 Sjofjan Osfar,

Adiansah I and

Sholiha K

Effect of Supplementation of Either Powdered or

Encapsulated Probiotic on Carcass Percentage,

Giblets and Small Intestinal Morphometric of

Local Duck

14.30-14.40

MVS-5 Sri Wahjuningsih,

Muhammad Nur

Ihsan, Gatot

Ciptadi and Helly

N. Karima

In vitro fertilization of cryopreserved goat oocytes

In different cryoprotectants

14.40-14.50





Theme : Medical & Veterinary Science (MVS)

Room : Arjuna 3

Time Keeper : 1. Rista Nikmatu Rohmah, M.Si

2. Nur Indah Ratnasari, S.Si


MVS-6 Chanif Mahdi, Ajeng E.P.

Haskito, Masdiana C.

Padaga and Anna Roosdiana

The Activity of Casein Derived

from Goat Milk Yogurt as An

Antioxidant on Histophatology of

Rat’s Liver Exposured by 2,3,7,8-

Tetrachlorodibenzo-p-Dioxin

(TCDD)

14.00-14.10

MVS-7 Trinil Susilawati, Aulia P.A.

Yekti, Kuswati, Wike A.

Suwito and Asri N. Huda

The Conception Rate of Artificial

Insemination by Using Sexing

Frozen Semen on Ongole

Crossbred Cows

14.10-14.20

MVS-8 Mudawamah M, I.D.

Ratnaningtyas, M.Z Fadli

and G. Ciptadi

Individual Mutations in

Indonesian Local Ettawah Goats

Based on The GDF9 Gene

14.20-14.30

MVS-9 Sri Rahayu, Widya A.

Prasdini, Mochammad S.

Djati, Sasangka P and Gatot

Ciptadi

Malondialdehyde (MDA) level

and protein profile of serum after

calving towards the provision of

selenium-vitamin ETM on dairy

cow Frisien Holstein (FH)

14.30-14.40

MVS-10 Nemay A Ndaong, Annytha

D, Nancy D.F.K Foeh, D.O

Marumata, Roni R.K,

Faustin M.K and Frans U.

Datta

The Profile of Red Blood Cells in

Sumba Ongole Cattle Is

Maintained in Dryland Areas

14.40-14.50



Poster Session


ID Presenter Title

P-01 Soon Hui Teoh, Katsuhiro

Miyajima, M. Shinohara, F.

Briand, Dai Nakae and

Takeshi Ohta

Pathophysiological Changes in Salt Loaded Female

Spontaneously Diabetic Torii (SDT) Fatty rats

P-02 Gabriella C. Krisnamurti,

Novia C, Christyanita P.E,

Yuli K. Br Tarigan, Azula V.D

and Fatchiyah F.

Interaction of Acetaminophen and Caffeine Towards

Cyclooxygenase-2 (COX-2) in Inhibition of Prostaglandin

(PGH) Synthesis

P-03 Aisyah L. Na’ima, Gadis M.

Sari and Dwikora N. Utomo

Effect of Combination Core Stability Exercise and

Contract Relax Exercise on Hamstring Flexibility

P-04 Dimas A. Nugraha, Ratna D.

Haryadi and Siswoyo

Cahyono

Direct Effect Short Wave Diathermy Therapy and

Exercise with Quadriceps Bench against Knee

Osteoarthritis Patients with Functional Capabilities

P-05 Mariko Maekawa, Tomohiko

Sasase, T. Saito, Y. Toriniwa,

Y. Ishii, A. Uemura, K.

Miyajima, Kinuko Uno, Yuki

Shirai, Dai Nakae, and T.

Ohta

Pathophysiological Evaluation of The Liver in Western

Diets Fed Insulin Resistant Zucker Fatty Rats

P-06 Mery Pattipeilohy and

Sundarinurha

The Immunogenicity of Polyclonal Antibody from

Induced Vibrio Parahaemolyticus Membrane by Elisa

Method

P-07 Nancy D.F.K Foeh, Nemay A.

Ndaog, Rambu E.M. Mala,

Elisabet B, Putri L.P, Annytha

D and Frans U. Datta

Isolation of Lactic Acid Bacteria from Ruments of Cattle

as a Starter in the Manufacture of Silage

P-08 Sundari, Khadijah, A.M Jayali

and N.H. Sukamto

Application of Barcode DNA rBcl Gen for the

Identification of Medicinal Plants: Red Jabon and Gofasa

P-09 Suzana, Melanny Ika

Sulistyowaty, Isnaeni and

Tutuk Budiati

Synthesis, Docking Molecule Study and Antibacterial

Activity of N’-(4Fluorobenzylidene) -4-

hydroxybenzohydrazide, and N’-(3-Bromobenzylidene) -

4hydroxybenzohydrazide



Keynote Speaker

Development of Health and Pharmaceutical Research Competitiveness toward SDGs

dr. Siswanto, MPH, DTM

Head, National Institute of Health Research and Development Ministry of Health, Republic of Indonesia

Sustainable Development Goals (SDGs), covering 17 Goals and 169 Targets, are basically to

address people, planet, and prosperity. There are 4 goals that are relating to health i.e. goal 2: zero hunger, goal 3: good health and well-being, goal 5: gender equality and goal 6: clean water and sanitation. Of course, goal 3: good health and well-being is the main goal of health system.

The ultimate goals of national health system are to improve the level of health (life expectancy), the quality of health services, and the equity of financial contribution (World Health Organization). To achieve such ultimate goals, the Ministry of Health has launched three strategies of health development i.e. healthy paradigm, improvement of access and quality of health services, and achievement of Universal Health Coverage. The strategies are basically aimed to strengthen national health system in all aspects i.e. health interventions, community empowerment, health financing, provision of drugs and medical devices, provision of health human resources, as well as, research and development.

Research and development, as a vehicle for innovation, is geared into national health problem priorities. The priorities of Indonesia health problems now are maternal and child health, nutrition (undernutrition, overnutrition), communicable diseases, non-communicable diseases, and access and quality of health care.

In maternal and child health, Indonesia is still facing high maternal mortality and high infant mortality rate. In the field of nutrition, Indonesia has double burden problems, in one hand Indonesia is facing undernutrition problem (underweight, stunting, and wasting), but on the other hand, the problem of obesity is continuously increasing.

In disease control, Indonesia is facing triple burdens, that is, (i) the shifting the burden of diseases from communicable to non-communicable, (ii) the unfinished homework of endemic communicable diseases (malaria, TB, dengue), (iii) the problems of new emerging and re-emerging communicable diseases (Avian Flu, MERSCoV, Ebola, MDR-TB, Leptospirosis, Filariasis, etc.).

In health financing, it is agreeable that the JKN system is able to improve the access to health care in a very significant way. However, it needs to improve a better system on how to control cost without sacrificing services quality and patient safety.

Looking at the problems stated, there are the abundance of opportunities on how to create innovations through research and development in solving such problems. With regard of health research, to simplify, it can be divided into two categories, i.e. research for health product and research for health system strengthening. Health products could be medicines, vaccines, biosimilar, immunotherapy, stem cells, functional foods, traditional medicines (herbs), as well as, medical devices.

For strengthening health system, operational research is suitable approach for evidence-based policies and programs. Problem solving approach is an appropriate model for operational research. The research cycle would cover (i) measuring the magnitude of health problems, (ii) identifying the causes of the problems, (iii) finding the solution to the problems, (iv) identifying the know-how of implementing the policy, and (v) evaluating the policy achievement. Here, applied research with a multidisciplinary approach is more suitable instead of basic research approach.

To do such research and innovations, it needs capable researchers, adequate research funding,



adequate laboratories, and researcher’s creativity. Creativity is the heart of innovation. However, innovation should be able to commercialize or to be applied in the field for better community welfare. To encourage research products to be commercialized, it is must a synergy between academicians/ researchers, business, government, and community (ABGC). The development phases should meet he normative phases of health-product development (the sequence of pre-clinical to clinical phases and good clinical practice), comply with health research ethic principles, as well as, be in line with regulatory requirements.

Keywords: SDGs, national priorities, triple burden of disease, double burden of nutrition, health system, research for health products, research for health system strengthening, problem solving, creativity, synergyKeynote Speaker



Keynote Speaker

The role of Ministry of Research, Technology, and Higher

Education (RISTEKDIKTI) in Coordinating and Directi ng the R & D on Health Products and Drugs for Utilization

Dr. Syafarudin, B.Eng., M.Eng.

Deputy Director of Industrial Technology Development – Energy & Transportation Science and technology is an effective instrument for advancing a nation, including Indonesia, in achieving a better national economic progress and prosperity. However, Science and technology generally cannot be mastered except through a very long process of R & D which is full of risks of failure. Therefore, the creation and management of a conducive R & D environment is the key to ensuring good relevance and productivity between R & D actors and the domestic medical and drug industries.

The R & D environment includes the existence of regulations that support researchers and research facilities, the availability of credible research institutions and is committed to always being a solution, the number of qualified researchers who have passion and focus in their fields, the adequacy of government and private R & D budgets (GERD) and the Government's partiality in using domestic R & D results.

Indonesia is running towards a more prosperous future and prosperity, through various alignments and the presence of the state in R & D. This can be seen from the increasing range of state support in R & D, various regulatory improvements such as the preparation of the 2017-2045 National Research Master Plan. The National Research Master Plan (RIRN) for 2017-2045 is prepared to harmonize long-term research needs with national development directions related to science and technology. RIRN is important because national development requires cross-sector planning to integrate all the interests of Ministries / Institutions, so that they are more effective and efficient in using limited R & D budgets in the desired time period. One of the focuses in RIRN is Health and Drug Research which is translated into several main themes, namely diagnostic and medical device development, bio-pharmaceutical product development technology, and drug raw material development (BBO) technology, all of which are intended to increase domestic capacity and reduce dependence on imports.

For this reason, the positive trend needs to be maintained through improvements to various national schemes and programs, especially by RISTEKDIKTI, which is responsible for the national R & D System. RISTEKDIKTI, particularly through the Directorate General of Research and Development Strengthening, needs to be smarter and continue to work hard to welcome the 4.0 Industry era with the spirit of open science, in order to increase the productivity and relevance of research in Indonesia.



PL-1

Recombinant Protein Production for Stem Cell Uses

Sumeth Imsoonthornruksa1,2, Rangsun Parnpai1, and Mariena Ketudat-Cairns1,2*

1Embryo Technology and Stem Cell Research Center and School of Biotechnology, Institute of Agricultural Technology, and

2Center for Protein Structure, Function and Application Suranaree University of Technology, Nakhon Ratchasima.

* E-mail address: [email protected]

To maintain and propagate undifferentiated mouse embryonic stem cells (ESCs) and human ESCs in in vitro culture requires media containing human leukemia inhibitory factor (hLIF) and basic fibroblast growth factor (hbFGF), respectively. The hLIF and hbFGF are now commercially available but still very expensive. The cost of cytokines and growth factors is one of the biggest issues for many laboratories. We recently reported an uncomplicated, fast and low-cost preparation of homemade soluble biologically active hLIF and hbFGF fusion proteins expressed in Escherichia coli. Under optimal conditions, one-step affinity chromatography was done to recover high purity (>95% pure) hLIF and hbFGF fusion proteins with the yields of 100-200 and 60-80 mg per liter of cell culture, respectively. The purified hLIF and hbFGF proteins, both with and without the fusion tags, were biologically active, which were confirmed by their ability to stimulate proliferation of TF-1 and NIH3T3 cellsin a dose dependent manner, respectively. The fusion proteins also have the ability to support several culture passages of undifferentiated mouse ESCs and human pluripotent stem cells, respectively. These results demonstrated that the N-terminal fusion tag of hLIF and hbFGF fusion proteins does not interfere with its biological activity and can be used directly in the stem cell culture without removing the fusion tags in a manner comparable to commercial hLIF and hbFGF, respectively. The use of these produced fusion proteins was found to significantly reduce the cost of media preparation in our laboratory approximately 30-60%. We have described a simple, reliable, cost effective and user-friendly method for in-house hLIF and hbFGF production, suggesting that it will have widespread usage as a tool in the production of several other growth factors and cytokines for stem cell research. We are also interested in using these growth factors and some others for wound healing and cosmetic uses.



PL-2

Orchestration of Housekeeping and Cell-Specific Metabolisms in Steroidogenic Cells by a Transcription Factor, Ad4BP/SF-1

Prof. Ken-Ichirou Morohashi, Ph.D

Molecular Biology, School of Medical Science, Kyushu University, Japan

Ad4BP/SF-1 (NR5A1) belongs to a nuclear receptor superfamily and was originally identified as a transcription factor that regulates steroidogenic genes. Indeed, many in vitro and in vivo studies have shown that Ad4BP/SF-1 regulates the transcription of nearly all steroidogenic genes by binding to their proximal promoters. Moreover, disruption of Ad4BP/SF-1 gene resulted in disappearance of the gonads and adrenal glands, suggesting that Ad4BP/SF-1 regulates genes essential for development and survival of the steroidogenic organs .

To identify whole target genes of Ad4BP/SF-1, we adopted chromatin immunoprecipitation followed by deep sequencing (ChIP-Seq) and transcriptomic analysis by mRNA-Seq. Our sequence datasets showed that nearly all glycolytic genes are regulated by Ad4BP/SF-1. Glycolysis followed by TCA cycle and oxidative phosphorylation produces ATP. Expectedly, knockdown of Ad4BP/SF-1 reduces ATP concentration. In addition to ATP, NADPH concentration was also decreased. Similar to glycolytic genes, genes required for NADPH production are found to be the target genes for Ad4BP/SF-1. Considering that ATP and NADPH are essential for cellular function and proliferation, it is plausible that insufficient production of ATP and NADPH results in disappearance of the steroidogenic cells.

In addition to glycolysis and NADPH production, our sequencing datasets above revealed that nearly all cholesterogenic genes are also the targets of Ad4BP/SF-1. Given that cholesterol is the starting material for steroidogenesis and is synthesized from acetyl-CoA, which is provided by glycolysis, and ATP and NADPH are consumed in cholesterogenic and steroidogenic pathways, our results possibly demonstrate that the biological processes required for steroid hormone synthesis is governed by Ad4BP/SF-1; Ad4BP/SF-1 orchestrates various metabolismic pathways to supply materials for steroidogenesis so that steroidogenic cells can accomplish them�particular function efficiently. This study provides the first example of a mechanism that orchestrates housekeeping and cell-specific metabolism at the transcriptional level.



PL-3

Effect of Radiation Exposure on Reproductive Function of Domestic and Wild Animals after the Fukushima Nuclear

Power Plant Accident

1Hideaki Yamashiro, 1Kazuki Komatsu, 1Kanna Meguro, 1Takuya Ohdaira, 1Takahisa Yamada, 2Yohei Fujishima, 2Goh Valerie Swee Ting, 2Kosuke Kasai, 3Akifumi Nakata,

4Kentaro Ariyoshi, 4Mitsuaki Yoshida, 5Manabu Fukumoto and 2Tomisato Miura

1Graduate School of Science and Technology, Niigata University, Niigata, Japan 2Graduate School of Health Sciences, Hirosaki University, Aomori, Japan

3Division of Life Science, Hokkaido Pharmaceutical University School of Pharmacy, Hokkaido, Japan 4Institute of Radiation Emergency Medicine, Hirosaki University, Japan

5Department of Molecular Pathology, Tokyo Medical University, Tokyo, Japan

The Fukushima nuclear power plant (FNPP) accident raised worldwide attention to the health risks of radiation exposure and to its potential transgenerational effects. The following questions are frequently asked: What does no immediate effects mean? Does it mean the radioactivity has been secured? How about the risk of cancer? Will the FNPP accident have an impact on offspring? Are low levels of radiation harmless for health? Is artificial radiation bad for health? Is internal exposure more dangerous than external exposure? We have established an archive system composed of livestock and wild animals in a 20-km radius around the FNPP, that is, the ex-evacuation zone of the FNPP accident. This system provides critical information for the understanding of radioactive contamination; environmental pollution, biodistribution and metabolism, the biological effects of internal and external exposure in association with dose evaluation. Here, we will present the effect of chronic and low-dose-rate radiation on reproductive function of domestic and wild animals after the FNPP accident.



PL-4

Pathophysiological Analysis of Diabetic Peripheral

Neuropathy in SDT Fatty Rat, a New Animal Model of Obese Type 2 Diabetes

Tomohiko Sasase, Ph.D

Biological/Pharmacological Research Laboratories, Central Pharmaceutical Research Institute JT Inc.

Aim : To characterize the diabetic peripheral neuropathy (DPN) in Spontaneously Diabetic Torii (SDT) fatty rat, a new obese type2 diabetic animal model with severe diabetic complications, we conducted functional and pathophysiological evaluations. Phlorizin, a sodium-glucose co-transporter inhibitor, was used to control the blood glucose levels. Methods: Male SDT fatty rats were treated the vehicle or phlorizin (100 to 150 mg/kg/day) from 5 to 16 weeks (n=6). Sprague-Dawley (SD) rats were used as an age-matched control (n=6). Body weights and biochemical parameters were periodically measured. The sensory and motor nerve conduction velocity (SNCV and MNCV) of the sciatic nerve, blood pressure, pupil size, and electrocardiogram were measured. At 16 weeks, rats were sacrificed, and sural nerve and intraepidermal nerve were sampled for the histological studies, transmission electron microscopic (TEM) analysis and determination of nerve fiber density. Results: SNCV and MNCV were delayed in the SDT fatty rats compared with the control SD rats (SNCV; SD 59.1±4.5 m/sec, SDT fatty 39.8±5.4 m/sec, MNCV; SD 49.3±13.0 m/sec, SDT fatty 41.2±8.5 m/sec). Intraepidermal nerve fiber density (IENFD) slightly decreased in the SDT fatty rats (SD 29.7±3.9 fiber/mm, SDT fatty 22.8±2.0 fiber/mm). In the TEM evaluation, the mitochondrial abnormalities and thinning of myelin sheath in small myelinated fibers, and vacuolation and mitochondrial swelling in unmyelinated fiber were found in the SDT fatty rats. Blood pressure increased (SD 112.7±8.8 mmHg, SDT fatty 142.2±15.2 mmHg) and the coefficient of variance of R-R (CVR-R) intervals tended to decrease in SDT fatty rats (SD 2.6±1.3%, SDT fatty 1.3±0.3%). The maximum pupil diameter responded to the mydriatic drugs in SDT fatty rats was smaller than that of SD rats (SD 5.3±0.2 mm, SDT fatty 4.8±0.3 mm). These changes, except the thinning of myelin sheath, were prevented by controlling blood glucose level with phlorizin treatment. Conclusion: Functional and pathological abnormalities in somatic and autonomic nerves were observed in male SDT fatty rats and were dependent on hyperglycemia. SDT fatty rats will be useful animal model in studies of the pathogenesis of DPN in type2 diabetes. References: 1. Journal of Pharmacological and Toxicological Methods, 88: 160-166, 2017. 2. Experimental Animals, 64: 161-169, 2015. 3. The Journal of Veterinary Medical Science, 74: 1669–1673, 2012.



PL-5

Pharmacological Responses to Anti-Diabetic Drugs in Female SDT Fatty Rats, A New Obese Type 2 Diabetic Rat

Takeshi Ohta, Ph.D Central Pharmaceutical Research Institute, JT Inc.

Introduction : Metabolic disorders, such as obesity and diabetes, have become global health problems, and this population of patients is rapidly increasing all over the world. Diabetic animal models are essential for obtaining a better understanding of diabetes and developing novel anti-diabetic drugs. The Spontaneously Diabetic Torii (SDT) fatty rat develops diabetes and its complications at a young age and has the potential to become an important animal model for obese type 2 diabetes. In this study, we investigated pharmacological responses to anti-diabetic drugs to elucidate the properties of female SDT fatty rats. Methods: The pharmacological effects of metformin and pioglitazone were investigated using female SDT fatty rats. Changes in biological parameters and histopathology were evaluated. Experiment 1; Metformin (100, 300 mg/kg) was administered to the rats from 5 to 9 weeks of age. Experiment 2; Pioglitazone (1, 10 mg/kg) was administered to the rats from 7 to 13 weeks of age. Results: Experiment 1; Metformin treatment led to good glycemic control, increases in sensory nerve conduction velocity, and improvements in pancreatic abnormalities such as irregular boundaries and vacuole form of islets. Experiment 2; Pioglitazone treatment decreased blood glucose and triglyceride levels; however, increases in body weight were observed. In Computed tomography (CT) analysis, both visceral and subcutaneous fat weights were also increased. In addition, pioglitazone treatment improved histopathological abnormalities, such as irregular boundaries and vacuole form of pancreatic islets, renal tubular vacuole form and extension, and hepatic fatty changes and hypertrophy. Conclusion: In female SDT fatty rats, the sub chronic administration of metformin or pioglitazone improved glucose/lipid metabolic disorders and histopathological lesions. The female SDT fatty rat is useful for development of novel anti-diabetic drugs. References: 1. Experimental Animals, 59 (1): 73-84, 2010. 2. Journal of Diabetes Research, 2014: Article ID 841957, 7 pages, 2014. 3. The Scientific Journal of Diabetology, 1(1): 8-13, 2017. 4. Medical Archives, 71(6): 380-384, 2017.



PL-06

Development of Recombinant Protein Based Biomarker for Autoimmune Diseases

Aulanni’am Aulanni’am

Research Group of Biopeptide, Immunodetection and Immunocontraception Faculty of Veterinary Medicine

Brawijaya University, INDONESIA [email protected]

Detection marker based on antigen antibody interaction had been widely used in the world due to its superior ability in knowing the disease marker, especially in the early phase before the clinical onset. The simplify techniques has been needed to detect the presence of early phase disease marker in order to be used by community without going to laboratory or medical facilities. Preparation of recombinant protein is one of the efforts to develop a simple and rapid technique test. Recombinant proteins also ensure uniform, and stable conditions over time. Recombinant protein-based material for Glutamic acid decarboxylase 65 kDA (GAD65), thyroid peroxidase (TPO) and thyroid stimulating hormone receptor (TSHR), also the matric metallo proteinase-3 (MMP3) were developed as marker to detect type 1 diabetes mellitus, autoimmune thyroid diseases (AITD) and rheumatoid arthritis (RA), respectively. This works involved the gene isolation from human blood, gene transformation and cultivation of competent bacterial cells to obtain gene product, also protein purification and product analysis. This product then used for immunochromathography based detection both reverse-flow or lateral methods. This effort could achieve the specificity, sensitivity, simple and rapid test that can apply in community. Recombinant protein-based material for rapid test also proposed economically and stability product so it can use by wider community in the world to support Sustainable Development Goals (SDGs) of Ensuring healthy lives and promote well being for all at all ages.

Keywords: recombinant-protein, autoimmune diseases, GAD65, TPO, TSHR and MMP3



MS-01

Isolation, Characterization Cancer Cells from Breast Cancer Patients

Wahyu Widowati1*, Yusuf Heriady2, Dian Ratih Laksmitawati3, Diana Krisanti Jasaputra1, Teresa Liliana Wargasetia1, Rizal Rizal4, Fajar Sukma Perdana4, Annisa Amalia4, Annisa

Arliyah 4, Zakiyatul Khoiriyah 4, Ahmad Faried5

1Medical Research Center, Faculty of Medicine, Maranatha Christian University, Bandung, West Java, Indonesia 2Faculty of Medicine, Bandung Islamic University, Bandung, Indonesia

3Faculty of Pharmacy, Pancasila University, Jakarta, Indonesia 4Biomolecular and Biomedical Research Centre, Aretha Medika Utama, Bandung, West Java, Indonesia

5Department of Neurosurgery and Stem Cell Working Group, Faculty of Medicine, Universitas Padjadjaran-Dr. Hasan Sadikin Hospital, Bandung, West Java, Indonesia

*Corresponding author: Wahyu Widowati /[email protected]

INTRODUCTION. The most case of cancer death which in the first ranks among cancers suffered by women is breast cancer. The breast cancer therapy for patients has been done but still not optimal, so it is necessary to understand the mechanism of therapy in the breast cancer cells model. This study aims to develop isolation techniques for cancer cells from breast cancer patients as a cancer cells model. METHODS. While the isolation of breast cancer cells is done by enzymatic methods using the collagenase I and hyaluronidase enzymes. The breast cancer cells characteristic based on the CD44/CD24 expression using positive control MDA-MB231 and MCF-7 breast cancer cell lines. RESULTS AND DISCUSSION. Observations on the 7th day showed that cells were adherent in fibroblastic form. Isolated breast cancer cells expressed 95.33±0.47% of CD44+/CD24+ were similar as MDA-MB231 breast cancer cell line. REFERENCE [1] Huang J, Wu L, Tashiro SI, et al. 2016. The augmentation of TNFα induced cell death in murine L929 fibrosarcoma by pan-

caspase inhibitor Z-VAD-fmk through premiochondrial and MAPK-dependent pathways. Acta Med. Okayama; 59: 253-260. [2] Torre LA, Bray F, Siegel RL, et al. 2015. Global cancer statistics, 2012. CA Cancer J Clin ;65(2):87-108. [3] Sheridan C, Kishimoto H, Fuchs RK, et al. 2006. CD44+/CD24- breast cancer cells exhibit enhanced invasive properties: an early

step necessary for metastasis. Breast Cancer Res., 8(5): R59.



MS – 02

Effectivty Of Betel Leaves (Piper Betle L.) And Breadfruit Leaves (Artocalpus Atilis) In Reducing Uric Acid Levels In

Hyperuricemic Male White Rats (Rattus Norvegicus)

Firman Faizal1, Donny Nauphar2, Tiar Masykuroh Pratamawati, Rama Samara Brajadenta3

1,2,3 Medical Faculty, Universitas Swadaya Gunung Jati

*Corresponding author:

INTRODUCTION. Hyperuricemia is a condition where plasma uric acid is elevated. Prolonged elevated uric acid levels increase the risk of gout. The prevalence of hyperuricemia is 20-25% in the general population. Herbal medication is an alternative therapy for gout with minimum side effects. Betel leaves and breadfruit leaves contain natural substances that may reduce uric acid levels. METHODS. This is a pre-and-post control group experimental research using 24 hyperuricemic male white rats divided into 4 groups. Negative control group were given normal feed and aquadest. Positive control group were given high-purine feed and aquadest. Group 1 and 2 were given high-purine feed and betel leaf extract of 332mg/200gBW and breadfruit leaf extract of 500mg/200gBW respectively. The data were analyzed using paired t-test. RESULTS AND DISCUSSION. Postive control group showed uric acid level of 8.01mg/dl and 8.25mg/dl (pre and post-test respectively). Betel leaf extract group showed a decrease in uric acid levels with post-test uric acid level of 2.02mg/dl from 8.09mg/dl, while breadfruit leaf extract group from 8.12mg/dl to 2.28mg/dl. REFERENCE [1] Sudoyo, Aru W: Buku Ajar Ilmu Penyakit Dalam Jilid III. Edisi VI. Jakarta: Internal Publishing; 2014. hlm.3179-3181. [2] Edwards L. The Role of Hyperuricemia In Vascular Disorders. Curr Opin Rheumatol. 2009;21(2):132-137. [3] Smith E, March L. Global Prevalence of Hyperuricemia: A Systematic Review of Population-Based Epidemiological Studies.

ACR Meet Abstr. 2015. [4] Meneses-leon J, Denova-gutiérrez E, Castañón-robles S, et al. Sweetened Beverage Consumption and The Risk of Hyperuricemia

In Mexican Adults: A Cross-Sectional Study. BMC Public Health. 2014:1-11. [5] Wang Y, Zeng C, Wei J, et al. Association between Dietary Magnesium Intake and Hyperuricemia. Plus One. 2015; 10 (11):1-10. [6] Badan Penelitian dan Pengembangan Kesehatan. Riset Kesehatan Dasar (RISKESDAS) 2013. Lap Nas 2013. 2013:94-96.



MS – 03

EFFECTIVITY OF TOMATO ( Solanum lycopersicum) AND ZINC COMBINATION TO SPERMS OF MALE WHITE RATS (Rattus norvegicus) EXPOSED TO MONOSODIUM

GLUTAMATE

Ignatius Hapsoro Wirandoko1*, Cici Apriyani 2, Dadan Ramadhan3

1Faculty of Medicine, Swadaya Gunung Jati University at Cirebon, email : [email protected] 2Faculty of Medicine, Swadaya Gunung Jati University at Cirebon, email : [email protected]

3Faculty of Medicine, Swadaya Gunung Jati University at Cirebon, email :[email protected]

*Corresponding author: [email protected]

INTRODUCTION .. Infertility is a condition of inability to conceive children. It occurs in more than 20% Indonesian population. Factors causing infertility include unhealthy lifestyle, such as excessive use of MSG which can produce free radicals and can affect morphology and motility of spermatozoa. Antioxidant is needed to prevent free radicals, which can be obtained from various sources such as tomatoes and zinc. METHODS . This is an experimental study with post test only control group design. Samples were divided into 5 groups, i.e. K1 which were given standard feed, K2 which were exposed to 4 mg/day of MSG, K3 which were given 3.7 g/day of tomatoes + 4 mg/day of MSG, K4 which were given 0.27 mg/day of zinc + 4 mg/day of MSG and K5 which were given combination of 3.7 g/day of tomatoes + 0.27 mg/day of zinc + 4 mg/day of MSG. RESULTS AND DISCUSSION. Tomatoes or zinc and the combination of tomatoes and zinc were effective to improve the morphology and motility of male white rats Rattus norvegicus spermatozoa which were exposed to MSG. REFERENCES [1] Alhassan et a. A survey on depression among infertile women in Ghana. J Women’s Heal. 2014.

[2] Soegiharto Soebijanto. Konsensus Penanganan Infertilitas daftar isi. Konsensus penanganan infertil. 2013.

[3] Riska RH, Edy SH PZ. Pengaruh pemberian zink (Zn) terhadap kualitas spermatozoa wistar jantan dewasa (Rattus norvegicus) yang di berikan monosodium glutamat (MSG). Farmakologi. 2012:29-36.

[4] Sukmaningsih a. a. S., Ermayanti IGAM, Wiratmini NI, Sudatri NW. Gangguan Spermatogenesis Setelah Pemberian Monosodium Glutamat Pada Mencit( Mus musculus L .). J Biol. 2009;XV(2):49-52.

[5] Radiyansyah G. Perbandingan efektivitas vitamin C,E,dan kombinasinya terhadap morfologi, motilitas, kuantitas dan viabilitas spermatozoa tikus putih (Rattus norvegicus) yang dipaparkan MSG (Monosodium glutamat). 2017.

[6] Lamarinde. Reactive Oxygen Species and Sperm Physiologi. PubMed; 2007.

[7] Larasati A. Efek protektif pemberian kombinasi tomat (Solanum lycopersicum L.) dan zink terhadap motilitas dan morfologi sperma tikus putih jantan (Rattus norvegicus L.) galur Sprague dawley yang diinduksi gelombang elektromagnetik telepon seluler. 2017.



MS - 04

The Level Short Chain Fatty Acids and HSP 70 in Colorectal Cancer and Non Colorectal Cancer

Fauzi Yusuf1*, Siti Adewiah2, and Fatchiyah3

1 Division of Gastroenterology-Hepatology, Department of Internal Medicine, Faculty of Medicine Universitas of Syiah Kuala/ Dr. Zainoel Abidin Centre Hospital, Banda Aceh, Indonesia.

([email protected]) 2 Department of Internal Medicine, Faculty of Medicine University of Syiah Kuala, Banda Aceh, Indonesia.

([email protected]) 3Research Center of Smart Molecule of Natural Genetic Resources, Department of Biology, Faculty of Mathematical

and Natural Science, Universitas Brawijaya, Malang, Indonesia ([email protected])


INTRODUCTION. Microbial involvement in colorectal cancer (CRC) is now well established. Short Chain Fatty Acids (SCFA) is the main products of anaerobic microbial fermentation in the large intestine and affects colonic health. SCFA mainly produced as microbial metabolites, acetate, propionate, and butyrate acids. Several in vitro studies showed that butyrate induce expression of heat shock protein (HSP) 70 that has function in the beginning of apoptosis. In this study, we investigate the differences level SCFA and HSP 70 expression in CRC compared with non-CRC patients. METHODS. The study consists of 14 patients diagnosed with CRC and 14 non CRC patients. Stool sample were analysed for SCFA (acetate, propionate, and butyrate acids) with gas chromatography and the result is given as µg/mL and the protein expression of HSP70 was determined by immunohistochemistry (IHC) and haematoxylin-eosin staining to determine the morphological changes in colon tissue. RESULTS AND DISCUSSION. We found that CRC patients had lower level of acetate, propionate and butyrate Acids than non-CRC. The mean concentration of acetate 8, 55 µg/mL, propionate 5, 61 µg/mL and butyrate acids 3, 79 µg/mL respectively (all P < 0.05) and among a sample of patients with colorectal cancer was obtained the highest expression of HSP-70.

REFERENCE [1] Chen HM, Yu YN, Wang JL, Lin YW, Kong X, Yang CQ, et al. Decreased dietary fiber intake and structural alteration

of gut microbiota in patients with advanced colorectal adenoma. Am J Clin Nutr. 97,1044–52 (2013). [2] Wu X, Yin Y, Zhang Y and Wu G. Roles of heat-shock protein 70 in protecting againts intestinal mucosal damaged.

Frontiers in Bioscienes. 18, 356-65 (2013). [3] Zeng H, Lazarova DL, Bordonaro M. Mechanisms linking dietary fiber, gut microbiota and colon cancer prevention.

World J Gastrointest Oncol. ; 6(2): 41-51 (2014).



MS – 05

Antimicrob activity of Whey Mare’s Milk Against Salmonella enteritidis

Annytha Detha1*, Agus Saputra2, Antonius Ola3

1Department of Animal Diseases and Veterinary Public Health, Veterinary Faculty, Nusa Cendana University, Kupang, Indonesia

2Divion of pharmacology and biochemistry, Veterinary Faculty, Nusa Cendana University 3Faculty of Engineering and Science, Nusa Cendana University, Kupang, Indonesia.


INTRODUCTION .. Sumba mare’s milk has good nutritional value with a balanced protein, fat and lactose content and therapeutic food sources because of high lactoferrin content. Lactoferrin is an important compound contained in milk whey protein. Lactoferrin has various biological functions, such as antimicrobial activity and to stimulate the formation of beneficial microflora in the gastrointestinal tract. METHODS The test was conducted at the Veterinary Public Health Laboratory of the Faculty of Veterinary Medicine, Nusa Cendana University. Pathogenic bacteria used are Salmonella Typhimurium ATCC® 19585 ™ and Salmonella Enteritidis ATCC® 13076 ™, obtained from the Veterinary Public Health Section, Faculty of Veterinary Medicine, Bogor Agricultural University. RESULTS AND DISCUSSION. The results show that Lactoferrin has antimicrobial activity against pathogenic bacteria Salmonella Typhimurium and Salmonella Enteritidis. Results of this research may be basic research to the development of lactoferrin from Sumba mare’s milk as an antimicrobial agent potential in treating Salmonellosis. REFERENCE [1] Detha A, Sudarwanto M, Latif H, Datta FUD, Puji L. 2013a. Fractionation and Identification Antimicrobial Activity of Sumba

Mare’s Milk Protein Against Subclinical Mastitis Bacteria in Dairy Cattle. Global Veterinaria 11(5):674-680. [2] Detha A, Sudarwanto M, Latif H, Datta FUD. 2014a. Kajian Komposisi Kimiawi dan Fraksinasi Protein Susu Kuda Sumba.

Jurnal Veteriner Jurnal Veteriner 1 (4), 506-514 [3] Lenka Ruprichová L, Králová M, Borkovcová I, Vorlová L, Bedáňová I. 2014. Determination of whey proteins in different types

of milk ACTA VET. BRNO 83: 067–072 [4] Nazzaro F, Orlando P, Fratianni F, Coppola R. 2010. Isolation of Components with Antimicrobial Property from the Donkey

Milk: A Preliminary Study. The Open Food Science Journal, 2010, 4, 43-47 43.



MS - 06

FAS and FASL Polymorphism and Susceptibility to Hepatitis

B Virus Infection in Javanese Individuals

Afiono Agung Prasetyo1,2*, Dyah Ayu Agustin1 1 A-IGIC (A-Infection, Genomic, Immunology & Cancer) Research Group, Sumber, Banjarsari, Surakarta, 57138,

Indonesia 2 Department of Microbiology, Faculty of Medicine, Universitas Sebelas Maret, Jl. Ir. Sutami 36A, Surakarta, 57126,

Indonesia *Corresponding author: [email protected]; [email protected]; [email protected]

INTRODUCTION. Hepatitis B virus infection is still a major global health problem. It is estimated there are 257 million people living with chronic liver disease currently caused by hepatitis B virus leading approximately 1 million deaths annually [1, 2]. The death receptor FAS and its ligand (FASL) has a central role in the physiological regulation of programmed cell death and has been implicated in the pathogenesis of various diseases [3-5]. Polymorphisms in FAS and FASL genes have been associated with susceptibility to hepatitis B virus infection in Iranian [6]. Therefore, the present study intended to investigate the occurrence of FAS and FASL polymorphisms and its role in the susceptibility to hepatitis B virus infection in Javanese individuals. METHODS . All blood samples with hepatitis B virus infection received but negative for other bloodborne pathogens (human immunodeficiency virus, hepatitis C virus, hepatitis D virus, Torque Teno virus, GB virus C, human T-cell lymphotropic viruses, and Toxoplasma gondii by serological and molecular assays) from previous studies [7, 8] was subjected for polymerase chain reaction-restriction fragment length polymorphism (PCRRFLP) assays as described previously [4, 6] to genotype the FAS-670 A/G and -1377 G/A, and FASL -844 C/T and -124A/G polymorphism status, respectively. As a control, a randomly 100 blood samples derived from healthy adults negative for all bloodborne pathogens were used. RESULTS AND DISCUSSION. The present study examined the occurrence of single nucleotide polymorphism (SNP) in FAS and FASL genes, especially the FAS-670 A/G and -1377 G/A, and FASL -844 C/T and -124A/G polymorphism status, and their association with the susceptibility to hepatitis B virus infection in Javanese individuals. The association of the genotypes for both FAS and FASL polymorphisms may contribute to the prediction of some effector immune response to eliminate the hepatitis B virus infection [4]. It is proposed that hepatitis B virus infection outcome in Japanese individuals might be influenced by FAS-670 A/G polymorphism through alteration in apoptosis of hepatocytes, differ with Iranian [5]. REFERENCE [1] Chauhan R, Lingala S, Gadiparthi C, Lahiri N, Mohanty SR, Wu J, Michalak TI, Satapathy SK. World J. Hepatol. 10, 352-70

(2018). [2] Susilawati TN, Setyawan S, Permana TY, Mudigdo A, Prasetyo AA. J. Phys.: Conf. Ser. 1022, 012050 (2018). [3] Guégan JP, Legembre P. F. E. B. S. J. 285(5), 809-27 (2018). [4] Vallinoto AC, Santana BB, dos Santos EL, Santo RR, Hermes RB, Sousa RC, Cayres-Vallinoto I, Machado LF, Ishak MO, Ishak

R. Virus Res. 163(1), 178-82 (2012). [5] Le Gallo M, Poissonnier A, Blanco P, Legembre P. Front. Immunol. 8, 1216 (2017). [6] Mohammadi A, Tajik N, Shah-Hosseini A, Alavian SM, Sharifi Z, Jarahi L. Hepat. Mon. 15(10), e26490 (2015). [7] Prasetyo AA, Dirgahayu P, Sari Y, Hudiyono H, Kageyama S. J. Infect. Dev. Ctries. 7, 453-467 (2013). [8] Prasetyo AA, Marwoto, Adnan ZA, Hartono. J. Phys.: Conf. Ser. 1022, 012044 (2018).



MS – 07

The Comparison on Antihyperglycemic Activity Between Gedong Gincu Mango Leaf (Mangifera Indica Var. Gedong

Gincu) And Metformin in Stretozotocin-Induced Diabetic Rats

Qurotul Aqyun 1, Ahmad Fariz Malvi Zamzam Zein2, Vivi Meidianawaty3

1,2,3Faculty of Medicine, Swadaya Gunung Jati University, Cirebon, West Jawa, Indonesia

Corresponding author: [email protected]

INTRODUCTION. Pharmacologic treatment is one of important modalities in the comprehensive management in type 2 diabetes mellitus, whereas metformin is the first line drug of choice in initiating oral antihyperglycemic agent. Several compounds like mangiferin, quersetin, tannins, & flavonoid have blood-glucose lowering properties. They are available in mango leaf. Gedong gincu mango is widely available in Cirebon, West Jawa, Indonesia, but the study enrolling its leaf in antihyperglycemic effect is not provided yet. This study was aimed to compare antihyperglycemic activity between gedong gincu mango leaf extract (Mangifera indica var. gedong gincu) and metformin on streptozotocin-induced diabetic rats. METHODS. This study was experimental research with pre- and post-test control group design enrolling 30 wistar streptozotocin-induced rats divided into 5 groups of treatment consists of 1 negative control group, and 4 positive control group using metformin 9mg/200gr and 3 groups given gedong gincu mango leaf extract with doses of 150 (P1), 300 (P2) and 600 (P3) mg/200 gr for 7 days. RESULTS AND DISCUSSION. There is significant decrease in blood glucose level in P1, P2, and P3 (96.24mg/dl, 116.62 mg/dl, and 136.21mg/dl). REFERENCES [1]Muruganandan S, Srinivasan K, Gupta S, Gupta PK, Lal J. Effect of mangiferin on hyperglycemia and atherogenicity in

streptozotocin diabetic rats. J Ethnopharmacol. 2005;97(3):497-501. [2] Syah M, Suwendar, Mulqie L. Uji aktivitas antidiabetes ekstrak etanol daun mangga arumanis (Mangifera indica L. “Arumanis”)

pada mencit Swiss webster jantan dengan metode tes toleransi glukosa oral (ttgo). Pros peneliti sivitas akad Unisba. 2015:297-303.



MS – 08

The Potential Role of Betatrophin in Hepatocellular

Carcinoma and Metabolic Syndrome

Hendra Susanto1*, A. Aulanni’am 2*, C. H Wang3, D. K. Wuragil 2, A. Y. Handaya4,5 M. P. Pertiwi1, S.A. Rufiatin Nisa1

1 Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Malang, Malang, Indonesia

2 Laboratory of Biosains, Faculty of Veterinary Medicine, Universitas Brawijaya, Malang, Indonesia 3 Department of Biological Science and Technology, National Chiao Tung University, Hsinchu, Taiwan ROC

4 Department of Digestive Surgeon Dr. Sardjito Hospital Yogyakarta, Indonesia 5 Faculty of Medicine, Universitas Gadjah Mada, Yogyakarta, Indonesia


INTRODUCTION. Metabolic syndrome is a fundamental health problem worldwide induced by obesity and T2DM. Obesity linked long-standing diabetes was established as a crucial event in cancer development. The recent findings improved that betatrophin, a novel liver-derived hormone, regulates lipid metabolism and glucose metabolism. However, the potential function of betatrophin activity in the pathogenesis of hepatocellular carcinoma (HCC) due to metabolic syndrome is not fully understood. This preliminary study aimed to elucidate the fundamental role of betatrophin in HCC linked metabolic syndrome. METHODS. This study was conducted by in vivo model by using miR-122a-/- mouse induced by a high-fat diet. The alteration of liver betatrophin expression was confirmed by real-time quantitative polymerase chain reaction (RT-qPCR) method, while the histological section examination was done to observe the change of liver anatomy. RESULT AND DISCUSSION. Importantly, our study found that diet-induced obesity (DIO) was able to accelerate metabolic syndrome. The long-term treatment of diet-induced obesity exacerbate portal inflammation, fatty liver and liver fibrosis to accelerate liver tumorigenesis or pre-cancer symptoms. The expression of betatrophin significantly increased in our metabolic syndrome mouse model indicate the involvement of betatrophin in lipid metabolism disorder to induce liver injury and hepatoma. Hence, this preliminary study proves a hallmark of the primary contribution of betatrophin in the early incidence of hepatocellular carcinoma linked metabolic syndrome. CONCLUSION. Therefore, further investigation is required to expand our hypothesis and theoretical framework. REFERENCES [1] Després J-P and Lemieux I 2006 Abdominal obesity and metabolic syndrome Nature 444 881-7 [2] Grundy S M, Brewer H B, Cleeman J I, Smith S C and Lenfant C 2004 Definition of metabolic syndrome report of the National

Heart, Lung, and Blood Institute/American Heart Association Conference on scientific issues related to definition Circulation 109 433-8

[3] Katzmarzyk P T, Church T S, Janssen I, Ross R and Blair S N 2005 Metabolic syndrome, obesity, and mortality Diabetes care 28 391-7

[4] Alberti K G M M, Zimmet P and Shaw J 2006 Metabolic syndrome—a new world�wide definition. A consensus statement from the international diabetes federation Diabetic medicine 23 469-80

[5] Eckel R H, Grundy S M and Zimmet P Z 2005 The metabolic syndrome The Lancet 365 1415-28 [6] Kahn R, Buse J, Ferrannini E and Stern M 2005 The metabolic syndrome: time for a critical appraisal Diabetologia 48 1684-99 [7] Nestel P, Lyu R, Low L P, Sheu W H-H, Nitiyanant W, Saito I and Tan C E 2007 Metabolic syndrome: recent prevalence in East

and Southeast Asian populations Asia Pacific journal of clinical nutrition 16 362-7 [8] Hsu W C, Araneta M R G, Kanaya A M, Chiang J L and Fujimoto W 2015 BMI cut points to identify at-risk Asian Americans for

type 2 diabetes screening Diabetes Care 38 150-8 [9] Cohen D H and LeRoith D 2012 Obesity, type 2 diabetes, and cancer: the insulin and IGF connection Endocrine-related cancer

19 F27-F45



MS - 09

The Inhibition of Vasa Vasorum Angiogenesis by Darapladib in Dyslipidemic Conditions

Teuku Heriansyah1, Aini, Fitria N 2, Nafisatuzzamrudah3, Primardhika, Rozah F.4, Wijayanti,

Maya D.5, Wihastuti, Titin A. 6

1Department of Cardiology, Faculty of Medicine, Syah Kuala University,Banda Aceh,Email: [email protected] 2Master Program of Biomedicine, Faculty of Medicine, Brawijaya University, Email: [email protected] 3Master Program of Biomedicine, Faculty of Medicine, Brawijaya University Email: [email protected]

4Bachelor Program, Faculty of Medicine, Brawijaya University, Email : [email protected] 5Bachelor Program, Faculty of Medicine, Brawijaya University, Email : [email protected]

6Department of Biomedicine, Faculty of Medicine, Brawijaya University, Email: [email protected]


INTRODUCTION . Atheroclerotic is a chronic inflammatory disease which leads to major cardiovascular events [1]. The primary cause of atherosclerosis is Dyslipidemia. Increased level of lipid profile triggers endothelial dysfunction, thus results in inflammation with the recruitment of monocyte, macrophage, T lymphosit, and Mast cell secreted by Lp-PLA2 enzyme which causes binding with oxidized LDL.Macrophage binds with oxLDL to form foam cell, and smooth cell migration. Lp-PLA2 receptor functions to hydrolize OxPC which results in LysoPC and OxNEFA, a bioactive compound which stimulates the progression of atherosclerotic plaques [2]. This process leads to tissue hypoxia, and results in the increased of HIF-α, VEGF expression and induces vasa vasorum angiogenesis. The agent of Lp-PLA2 selective inhibitors in this study is Darapladib. METHODS . This study is to understand the correlation between darapladib and formation of vasa vasorum angiogenesis, HIF-1α and VEGF expression in aortic tissue of rats with dyslipidemia. Data measured in this study is the lipid profile (total cholesterol, HDL, and LDL). True experimental laboratory with randomized post test control group design with 30 rats was divided into 6 groups: Normal, Dyslipidemia (DL) and Dislipidemia with darapladib injection (DLDP), each group consists of 2 time serial, 8 and 16 week. RESULTS. Study result using ANOVA test showed a significant darapladib injection with decreased vasa vasorum angiogenesis (p=0.000), HIF-1α (p=0.005) and VEGF (p=0.009) expression in each time series. This result proves Lp-PLA2 inhibition reduces inflammatory process. In conclusion, darapladib injection as Lp-PLA2 selective inhibitors correlates with decreasing vasa vasorum angiogenesis through alteration in HIF-1α and VEGF expression in the aorta of high fat diet rats. We recommend further experiments to determine the effectiveness of darapladib with earlier time series in atherosclerotic process.

REFERENCES [1] Mendis, S., Puska, P; Norrving, B. Global Atlas on Cardiovascular Disease Prevention and Control. World Health Organization,

2011, 1-153 [2] Koenig, W., Twardella, D., Brenner, H., 2006. Lipoprotein-Associated Phospholipase A2 Predicts Future Cardiovascular Events

in Patients With Coronary Heart Disease Independently of Traditional Risk Factors, Markers of Inflammation, Renal Function, and Hemodynamic Stress. Arteriosclerosis, Thrombosis, and Vascular Biology, 26(7): 1586–93



MS – 10

The Effects of Specific Inhibitor Lp-PLA2 on Vasa Vasorum Angiogenesis Through Inhibition Vascular Endothelial Growth Factor Expression: Study In Vivo Using Type 2

Diabetes Mellitus

Titin Andri Wihastuti 1*, Nafisatuzzamrudah2, Fitria Nugraha Aini 3, Kenty Wantri Anita 4, Sari Kushardianti 5, Sandra Pradita Widya Aswuri6 Teuku Heriansyah7

1Department of Biomedicine, Faculty of Medicine, Brawijaya University, Email: [email protected] 2Master Program of Biomedicine, Faculty of Medicine, Brawijaya University, Email: [email protected] 3Master Program of Biomedicine, Faculty of Medicine, Brawijaya University Email: [email protected]

4Department of Patology Anatomy, Faculty of Medicine, Brawijaya University, Email : [email protected] 5Bachelor Program, Faculty of Medicine, Brawijaya University, Email : [email protected]

6Bachelor Program, Faculty of Medicine, Brawijaya University, Email : [email protected] 7Department of Cardiology, Faculty of Medicine, Syah Kuala University, Banda Aceh, Email:

[email protected]


INTRODUCTION . Insulin resistency decreases endothelin’s ability to release NO, O2, and other substances which causes inflammation and endothelial dysfunction [1]. As a result, atherosclerotic plaques are easily formed. Hypoxia due to arterial wall thickening causes increase of vasa vasorum neo-vascularization and HIF-1α secretion which in turn induce the production of VEGF causing angiogenesis. Darapladib is a Lp-PLA2 inhibitor predicted to slow atherosclerosis progressivity [2,3]. METHODS . Experimental study using post test with control group in vivo to Sprague Dawley species. Divided into 3 groups, 1) control groups, 2) type 2 diabetes mellitus group with high fat diet feeding and 3) type 2 diabetes mellitus with high fat diet feeding and darapladib administration. Measured parameter are glucose level, plasma insulin level, insulin resistency, lipid profile, amount of vasa vasorum and VEGF expression. RESULTS. In one-way ANOVA test showed darapladib effect have a significant differences (p<0.05) to total cholesterol lipid profile (p=0.049), HDL (p=0.000), LDL (p=0.000), amount of vasa vasorum (p=0.000), but not significant in VEGF expression (p=0.375). Darapladib administration has ability to decrease lipid profile and amount of vasa vasorum. However, in VEGF expression, there is no significant effect. This findings is probably due to another pathway besides VEGF mediated pathway. Further studies are required to understand compensatory pathways and their effects on artherosclerotic treatment.

REFERENCES [1] Heriansyah, T., Wihastuti, T. A., Sargowo, D., Widodo, M. A., Anjani, P. M., Suparno, T. D., Puspitasari, F. I. 2014. Reduction

Of Histopathological Images Through A Decrease In H2O2 Levels In Diabetic Rats With Polysaccharide Peptides. Biomarkers and Genomic Medicine, 1–7.

[2] Mohler ER, 3rd, Ballantyne CM, Davidson MH, Hanefeld M, Ruilope LM, Johnson JL, and Zalewski A. Darapladib Investigators (2008). The effect of darapladib on plasma lipoproteinassociated phospholipase A2 activity and cardiovascular biomarkers in patients with stable coronary heart disease or coronary heart disease risk equivalent: the results of a multicenter, randomized, double-blind, placebo-controlled study. J Am Coll Cardiol 51:1632–1641.

[3] Serruys, P.W., García, H.M., Buszman, P., Erne P., Verheye, S. et al., 2008. Effects of the Direct Lipoprotein Associated Phospholipase A2 Inhibitor Darapladib on Human Coronary Atherosclerotic Plaque. Circulation AHA Journal, 118(11): 1172–82.



MS – 11

Albumin and Enzyme Profile of Dwarf Snakehead, Channa gachua were Caught from the River in East Java

Anik M. Hariati, A. Yuniarti, D.G.R. Wiadnya and W. E. Kusuma

Aquatic Biofloc Research Group Faculty of Fisheries and Marine Sciences University of Brawijaya Jalan Veteran Malang, Indonesia


INTRODUCTION. Albumin is one of the functional foods that are needed in the health sector, especially to accelerate the wound healing process, regulate osmotic pressure and as an antioxidant. The source of albumin from fishery products comes from snakehead fish. The albumin content in the fish's body can be influenced by the stage of development indicated by the size of the fish. The purpose of this reseach was to obtain albumin and enzyme profile data on various fish sizes. METHODS . In this study measurements of albumin and enzymes at various lengths of fish caught in the river were divided into three ranges, including less than 10 cm, 10-15 cm and more than 15 cm. Whereas fish identification is carried out molecularly with specific primers. RESULTS AND DISCUSSION. The results showed that albumin levels, and enzymes varied based on fish size. The average albumin level ranged between 10.22-14.75% and the highest in fish size more than 15 cm (increased 40% compared to small size). In contrast to lipase levels ranging between 200-225 µmol g-1 and protease 0.40-0.52 µmol g-1, decreased with the length of the fish. CONCLUSION. The longer the size of the fish, the higher the content of albumin, in contrast to the content of digestive enzymes. Identified fish tend to be Channa gachua.

REFERENCE [1] Firlianty, Suprayitno E, Nursyam, Hardoko and Mustafa A. Chemical composition and amino acid profile of channidae collected from central Kalimantan, Indonesia IEESE International Journal of Science and Technology 2(4) pp 25-9 (2013) [2] Asfar M, Tawali A B, Abdullah N. Extraction of albumin of snakehead (ChannaStriatus) in producing the fish protein concentrate (FPC) Int Journal of Scientific &technology Research 3(4) pp 85-8 (2014) [3]Scott D, Moore J, Herborg L-M, Clarke Murray C, Serrao N. A non-native snakehead fish in British Columbia, Canada: capture, genetics, isotopes, and policy consequences. Manag Biol Invasion. 4(4):265–71. (2013)



MS – 12

Protein Profile And Hemagglutination Activity of Pi lli, an Adhesion Factor Causing Typhoid Fever by Salmonella typhi

Sri Darmawati1, Stalis Norma Ethica2, Sri Sinto Dewi3

1Laboratory of Microbiology, Faculty of Nursing and Health, Universitas Muhammadiyah Semarang, Semarang, Indonesia, [email protected]

2Laboratory of Molecular Biology, Faculty of Nursing and Health, Universitas Muhammadiyah Semarang, Semarang, Indonesia, [email protected]

3Laboratory of Microbiology, Faculty of Nursing and Health, Universitas Muhammadiyah Semarang, Semarang, Indonesia, [email protected]

Corresponding author: Sri Darmawati, [email protected]

INTRODUCTION Typhoid fever is an infectious disease caused by Salmonella typhi (S. typhi). The infection was initiated by attachment of bacterial pilli to host cell. Pilli contains pillin proteins, which known to have hemagglutination activity. This study aims at analyzing protein profile of pilli belonged to two S. typhi isolates, BA07.4 and KD30.4, and assessing their hemagglutination activity on human red blood cells. METHODS Profile of pilli proteins extracted from two S. typhi isolates (BA07.4 and KD30.4) were observed using SDS-PAGE 12% method, stained with 0.1% coomasie brilliant blue solution. Hemagglutination activity of the isolated pilli proteins was tested on four erythrocyte of human blood groups, i.e. A, B, AB, and 0 using dilution method. RESULTS AND DISCUSSION Results showed that SDS-PAGE analysis on pilli proteins of S. typhi BA07.4 resulted two major protein subunits sized 87 and 42 kDa along with 14 minor ones. Menwhile, pilli proteins of S. typhi KD30.4 isolate showed two major protein subunits sized 87 and 42kDa along with 18 minor protein subunits observed on the SDS-PAGE. From the hemagglutination activity of S. typhi BA07.4 on the studied blood groups of A, B, AB, and 0 was 128, 2048, 64, and 2 HA (Hema-Agglutination), respectively. On the other hand, the hemagglutination activity of S. typhi KD30.4 on the studied blood groups of A, B, AB, and 0 was 8, 64, 1024, and 0 HA, respectively.



MS – 13

The Comparison on Antihyperglicemic Effectiveness Comparison Of Ethanol Extract Of Soursop Leaf (Annona

Muricata L.) Againts Acarbose in Streptozotocin-Induced Diabetic White Rats

Ruri Rizqiah Setiadi1, Ahmad Fariz Malvi Zamzam Zein2, Donny Nauphar3

Faculty of Medicine, Swadaya Gunung Jati University, Cirebon, West Jawa, Indonesia

INTRODUCTION . Type 2 diabetes mellitus is major health problem worldwide, including in Indonesia. Acarbose, an alpha glucosidase inhibitor, is one of oral pharmacologic management commonly used in clinical practice with potent side effect in long-term use. Flavonoid-and-tannins containing soursop leaf can stimulate insulin secretion and repair beta pancreatic cell dysfunction. This study was aimed to investigate the comparison on antihyperglycemic effect between ethanol extract of soursop leaf (Annona muricata L.) and acarbose in streptozotocin-induced diabetic white rats. METHODS . this sudy was experimental research with pre- and post-test control group design enrolling 30 hyperglycemic white male rats. They were divided into 2 control groups (C(+) was given by aquades and C(-) was given by acarbose) and 3 treatment groups (P1 was given 10 mg/200gBW extract, P2 was given 20 mg/200gBW extract, and P3 was given 30 mg/200gBW extract). Data analysis was using paired-T test. RESULTS. This study showed that there is a significant decrease in blood glucose levels in P1, P2, and P3 (95.08 mg/dL, 113.31 mg/dL, and 125.17 mg/dL; p<0.05).

REFERENCES [1] Justino AB, Miranda NC, Franco RR, Martins MM, da Silva NM, Espindola FS. Annona muricata Linn. leaf as a source of

antioxidant compounds with in vitro antidiabetic and inhibitory potential against a-amylase, a-glucosidase, lipase, non-enzymatic glycation and lipid peroxidation. Biomedicine & Pharacotherapy. 2018: 100; 83-92.

[2] Brindis F, Gonzalez-Trujano ME, Gonzalez-Andrade M, Aguirre-Hernandez #, Villalobos-Molina R. Aqueous extract of Annona macroprophyllata: a potential a-glucosidase inhibitor. Biomed Res Int. 2013;2013:591313. Epub 2013 Nov 4.



MS – 14

Cutaneous Wound Healing Activity of Herbal Ointment Containing the Leaf Extract of Acalypha indica L. on mice

(Mus musculus)

Meity Laut 1*, Nemay Ndaong1, Tri Utami 2 1Division of Pharmacology and Toxicology, Faculty of Veterinary Medicine, Nusa Cendana University, Kupang,

Indonesia 2Division of Surgery and Radiology, Faculty of Veterinary Medicine, Nusa Cendana University


INTRODUCTION . Acalypha indica L. is a tropical weed known as medicinally important plant. The analgesic, antiinflammatory, antibacterial, antiviral, and antifungal properties have already been reported for this plant. The present study evaluated the potential wound healing activity of ethanol leaf extract of Acalypha indica L topical formulation using mice excision wound models. METHODS . Ten healthy male mice aged 3-4 months, weighing between 20-30 g were used in this study. Ethanol leaf extract of A. indica was formulated as 5%, 10% and 20% ointment. Blank ointment base (vaselin album) and betadine 10% ointment served as the control and standard treatment, respectively. The parameter measured were percentage of wound contraction and epithelization period. RESULTS. The mice group treated with 5% and 10% extracts ointment showed better reduced the wound area compared to the negative control. However, it was only 10% of the extract ointment significantly shortened epithelization period. CONCLUSION. These findings collectively indicate that ointment of ethanol leaf extract of A. indica could facilitate wound healing, providing support for the traditional claims of the plant in the treatment of wound.

REFERENCES [1]Kumar B, Vijayakumar M, Govindarajan R, Pushpangadan P. 2007. Review: Ethnopharmacological approaches to wound healing

– exploring medicinal plants of India. Journal of Ethnopharmacology,Vol 114 (2), pp. 103-113. [2]Mohideen SK, Selvan T, Sheriff MA, Azmathullah Md. 2010. Phytochemical Screening of Acalypha Indica L. Leaf Extracts.

International Journal of Applied Biology and Pharmaceutical Technology. Vol 3 (2), pp. 158-161. ISSN: 0976-4550. [3]Rahman MA, Bachar SC, Rahmatullah M. 2010. Analgesic and Antiinflammatory Activity of Methanolic Extract of Acalypha

Indica Linn. Pak. J. Pharm. Sci., Vol.23, No.3, July 2010, pp.256-258 [4]Tariq AL, Priya U, Lone RA. 2015. Medicinal Plants Acalypha indica and Prosopis gladulosa an Alternative Medication for

Candidiasis. Int.J.Curr.Microbiol.App.Sci Vol. 4 (8), pp. 343-351 [5]Young A, McNaught C. 2011. The physiology of wound healing: Basic Science Surgery. Elsevier. pp. 475-479.



MS – 15

Terbufos and Fenthion Induce DNA damage and Active Homologous Recombination Repair pathway in vitro and in

vivo

Eva Ari Wahyuni 1,2,3*, Chiou Hwa Yuh4, and Ssu-Ching Chen2 1 Joint Ph.D Program in Molecular Medicine, National Health Research Istitutes & National Central University, Taiwan

2 Education Faculty, Trunojoyo University Madura, Indonesia 3Department of Life Sciences, National Central University, Taoyuan, Taiwan

4 Division of Molecular and Genomic Medicine, National Health Research Institutes, Miaoli,Taiwan


INTRODUCTION . Terbufos (S-tert-butylthiomethyl O-O-diethyl phosphorodithioate) and fenthion are a widely used worldwide as an insecticide and nematicide. Both of chemicals also represent cellular DNA damage through reactive oxygen species (ROS) generation. However, the mechanism of DNA damage mediated response of DNA repair pathway due to both of that chemicals (terbufos, fenthion and mixture terbufos and fenthion) exposure is not elucidated yet. In addition, combined effects considering to estimate adequately their combined adverse effects. METHODS. Therefore, we exposed HepG2 cells to different low concentration (5µM – 40 µM) of terbufos and fenthion for 24h and 48h, DNA damage and cell viability were analyzed in vitro. Moreover, MTT assay and comet assay revealed the significant decrease in cell viability and increase in DNA damage in single exposure terbufos and fenthion (10 µM – 40 µM), however combining pesticides increased cell viability and failed to induce significant DNA damage (5 µM -40 µM). RESULT AND DISCUSSION. Interestingly, the exposure of terbufos or fenthion could cause DNA double-strand breaks using the neutral comet assay.

REFERENCE [1] Hung, J.H., Chia-Yun Chen, Hany A.Omar, Kuo-Yuan H. Che-Chia T.,Chien-Chih Chiu, Yi-Ling C., Po-Han C., Yen-Ni T.

2015. Reactive oxygen species mediate terbufos- induced apoptosis in mouse testicular cell lines via the modulation of cell cycle and pro-apoptotic proteins. Environmental Toxicology DOI 10.1002/tox.

[2] Liang, Y., Fang T., Lin Z., Wenhe L., Weisheng H., and Yiwu Z., 2017. Fatal poisoning by terbufos following occupational exposure. Clinical toxicology.

[3] Niu, Yujie, Xing Zhang, Yuxin Zheng and Rong Zhang. 2013. XRCC1 deficiency increased the DNA damage induced by –ray in HEpG2 cell: Involvement of DSB repair and cell cycle arrest. Environmental Toxicology and Pharmacology 36: 311-319.

[4] Shaik, A.S., Abjal P.S., Kaiser J., and Abbas H.A., 2016. Evaluation of cytotoxicity and genotoxicity of pesticide mixtures on lymphocytes. Toxicology mechanisms and methods: 1-7. DOI: 10.1080/15376516.2016.1218577.



MS – 16

Diabetes Dance of Persadia 1 Effect on Blood IL-6 Level

Wahyu N.Pratiwi1*,Bambang Purwanto2,Abdurachman3

1Department of Sport Health Science, Airlangga University, Surabaya, [email protected]

2Department of Physiology Science, Airlangga University, Surabaya, [email protected] 3Department Anatomy and Histology, Airlangga University, Surabaya,[email protected]


INTRODUCTION. Diabetes dance of Persadia 1 is the evolvement of prior diabetes dance series with aerobic principle and aims to improve glucose tolerance and decrease HbA1c and belong to weight bearing exercise with more eccentric movement. Diabetes dance of Persadia 1 has been inspected effective for lowering fasting blood glucose compare to prior series of diabetes dance, but its still unknown about its effect on blood level of interleukin-6 (IL-6). The aim of this research is to analyze the effect of Diabetes dance of Persadia 1 on blood level of IL-6. METHODS. This research is experimental, with 16 subject of healthy housewifes who was participated and divided on 2 groups, that is exercise group and control group. 8 subjects were randomly assigned each group. Exercise group was given with diabetes dance of Persadia , 3 times per week for 2 weeks, while control group have the unstructured activity. Both control and exercise group already have their conditioning phase for 2 weeks before they start their training. Blood level of IL-6 has been measured from each subject’s blood that has been taken after 24 hours post single bout exercise (acute-AE) and after repeated exercise (chronic exercise-CE). RESULTS AND DISCUSSION. Paired t-test analysis on both acute (p=0,674) and chronic (p=0,949) shows blood level of IL-6 increase on control group post test result , though its not significant, while it has been decrease significantly on exercise group both AE (p=0,000) and CE (p=0,000). This can happen because of the stimulation of endogenous antioxidant production that is gluthatione. Aerobic exercise can significantly increase the level of glutathione peroxidase, thus lowering oxidative stress biomarkers by suppressing H2O2 production, resulting in NFkB inhibition. NFkB inhibition will decrease the production of IL-6 muscle.

REFFERENCE [1] Abdullah, Ahmad, Herdianty Kusuma, Elyana Asnar, Bambang Purwanto. 2017. Shoe Insole For Eccentric Activities On Daily

Application Prevents Muscular Damage And Improves Fasting Blood Glucose. Folia Medica Indonesiana, Vol 53, No 4. http://dx.doi.org/10.20473/fmi.v53i4.7151

[2] Ahmadian, Mehdi, Valiollah Dabidi Roshan, Anthony S. Leichtc. 2018. Age-related effect of aerobic exercise training on antioxidant and oxidative markers in the liver challenged by doxorubicin in rats. Free Radical Research. https://doi.org/10.1080/10715762.2018.1470328

[3] Gosset, Philippe, Wallaert B, Tonnel AB, Fourneau C. 1999. Thiol regulation of the production of TNF-a, IL-6 and IL-8 by human alveolar macrophages. European Respiratory Journal, 14: 98-105

[4] Pranoto, Agung. 2017. Senam Persadia 1, video Senam Persadia 1. Surabaya: Persatuan Diabetes Indonesia [5] Wibisono, Sony. 2017. Surabaya International Physiology Symposium. Surabaya: Universitas Airlangga



MS – 17

Effect of Torbangun (Coleus amboinicus Lour) on Blood Pressure in Women with Hypercholesterolemia

Trini Suryowati 1*, Moskwadina Gultom2

1*Department of Biochemistry, Faculty of Medicine, Christian University of Indonesia, Jakarta 13630, Indonesia 2Department of Histophatology, Faculty of Medicine, Christian University of Indonesia, Jakarta 13630, Indonesia


INTRODUCTION. Dietary polyphenols have been demonstrated to modify a number of cardiovascular risk markers such as blood pressure (BP), endothelial function and plasma lipids. Torbangun (Coleus amboinicus Lour) is known for its medicinal properties, as antihyperlipidemia, antihyperglicemia. We conducted a randomised, double-blind, controlled to investigate the effect of a phenolic-rich Torbangun leaves aqueous extract on woman with hypercholesterolemia. METHODS . Design experiment used in this study was pre and post-test controlled group design. The first step of this study was analyzed bioactive compound of Torbangun aqueous extract. The next step was administired orally of Torbangun extract to control and woman with hypercholesterolemia for 30 days, with 30 subjects placed in two groups, viz, experimental (CC group) and placebo (PC group). The first group received one capsules of CC (each capsule contains 500 mg extract) while the second group received one capsules of PC (comprised of 500 mg fillers) one times daily for both groups. Blood pressure (BP) and total cholesterol (TC) were measured in the first and the end of intervention. RESULTS. Oral administired of Torbangun extract exhibited reduce of total cholesterol, body mass index but not blood pressure in woman with hypercholesterolemia. Data were analyzed with paired sample t test with 95% significance level. The results show that there was significant decrease in TC on day 30, compared to control (PC group), and not significant for BMI. In CC group, the capsules significant reduction in TC levels (p=0.000). Studies clearly demonstrated that Torbangun extract possesses antihypercholesterolemia activity in woman with hypercholesterolemia. Based on the results of this study Torbangun extract reduce BMI but has not antihypertensive property.

REFERENCES [1] Lu, C. H. and Hwang, L. S. Polyphenol contents of Pu-Erh teas and their abilities to inhibit cholesterol biosynthesis in Hep G2

cell line. Food Chemistry. 111: 67-71. (2008) [2] Stapleton PA, Goodwill AG, James ME, D’Audiffrent AC, Frisbee JC. Differential impact of familial hypercholesterolemia and

combined hyperlipidemia on vascular wall and network remodeling in mice. Microcirculation. 17:47-58 (2010)



MS – 18

Improved Hypoglycemic Effect of Anthocyanin Extract Combination from Red Rice And Black Soybean

Wahidah Mahanani Rahayu1*, Mary Astuti 2, Yustinus Marsono2

1Department of Food Technology, Faculty of Industrial Technology, Universitas Ahmad Dahlan, Prof. Dr. Soepomo, S.H. Street, Warungboto, Janturan, Umbulharjo, Yogyakarta 55164

2Department of Food and Agriculture Product Technology, Faculty of Agricultural Technology, Universitas Gadjah Mada, Flora Street 1, Bulaksumur, Caturtunggal, Depok, Sleman, Yogyakarta 55281


INTRODUCTION. Hyperglycemia is the symptom for impaired glucose metabolism which leads to oxidative stress, diabetes mellitus type 2, and organs failure. As red rice and black soybean have long been part of diabetic patient diet, possible synergetic anti-hyperglycemic effect of anthocyanin extract combination from the two sources was studied. This study aimed to investigate hypoglycemic effect of crude anthocyanin extract from aleurone of red rice (Oryza sativa var. Mandel Handayani) and seed coat of black soybean (Glycine max var. Mallika). METHODS. Observation was conducted on glucose level, total plasma antioxidant capacity using Ferric Reducing Ability of Plasma (FRAP), antioxidant enzyme superoxide dismutase (SOD) activity, liver glycogen content, and pancreatic beta cell of streptozotocin-nicotinamide induced hyperglycemic 2-months old male Sprague Dawley rats. Thirty rats were divided (n=6) into two groups of healthy and hyperglycemic control, and three hyperglycemic groups orally administered with daily dose of 100 mg/kg body weight of anthocyanin extract from red rice, black soybean, and combination at 1:1 (w/w) ratio for 35 days. RESULTS AND DISCUSSION. Results showed that consumption of anthocyanin extract significantly reduced blood glucose by 45.3%-50.9%, prevent body weight loss and increase feed efficiency ratio by 36.42-51.52%. Total antioxidant capacity was also 15.84 – 25.30% higher, while SOD activity was higher in treated groups, only reduced by 16.3 – 54%, compare to significantly lower results of hyperglycemic group. Synergetic effect of combination extract was indicated by 45.3%-50.9% lower blood glucose and higher SOD activity in treated groups.

REFERENCE [1] Anonym. (2013). Riset Kesehatan Dasar Riskesdas (Basic Health Survey) 2013. Jakarta: Research and Development Center of

Indonesian Ministry of Health. [2] DeFronzo, R.A. (2009). From the triumvirate to the ominous octet: a new paradigm for the treatment of type 2 diabetes mellitus.

Diabetes 58: 773–795. [3] Salway, J.G. (2004). Metabolism at A Glance, 3rd ed. Blackwell Publishing, Oxford, United Kingdom. [4] Nandi, A., Chatterjee, I.B. (1988). Assay of superoxide dismutase activity in animal tissues. Journal of Bioscience, 13(3): 305–

315. [5] Robertson, R. P., Harmon, J. (2006). Diabetes, glucose toxicity, and oxidative stress: A case of double jeopardy for the pancreatic

islet β cell. Free Radical Biology and Medicine 41: 177 – 184. [6] Fernandes, I., Nave, F., Gonçalves, R., de Freitas, V., Mateus, N. (2012). On the bioavailability of flavanols and anthocyanins:

Flavanol-anthocyanin dimers. Journal of Food Chemistry, 135: 812–818. [7] Nizamutdinova, I.T., Jin, Y.C., Chung, J.I., Shin, S.C., Lee, S.J., Seo, H.G., Lee, J.H., Chang, K.C., Kim, H.J. (2009). The anti-

diabetic effect of anthocyanins in streptozotocin-induced diabetic rats through glucose transporter 4 regulation and prevention of insulin resistance and pancreatic apoptosis. Molecular Nutrition and Food Research 53:1419–1429.

[8] Takikawa, M., Inoue, S., Horio, F., Tsuda, T. (2010). Dietary anthocyanin-rich bilberry extract ameliorates hyperglycemia and insulin sensitivity via activation of AMP-activated protein kinase in diabetic mice. The Journal of Nutrition 140: 527-533. [9] Scazzocchio, B., Vari, R., Filesi, C., D'Archivio, M., Santangelo, C., Giovannini, C. (2011). Cyanidin-3-O-beta-glucoside and

protocatechuic acid exert insulin-like effects by upregulating PPARγ activity in human omental adipocytes. Diabetes 60: 2234-2244.

[10] Fitri Kharismawati. (2015). Dampak Konsumsi Ekstrak Antosianin Beras Merah, Kulit Kedelai Hitam, Dan Campurannya Terhadap Profil Lipid Tikus Hiperglikemia. Thesis. Program Studi Ilmu dan Teknologi Pangan, Fakultas Teknologi Pertanian, Universitas Gadjah Mada, Yogyakarta.

[11] Shanmuganayagam, D., BeahM, M.R., Osman, H.E., Krueger, C.G., Reed, J.D., Folts, J.D. (2002). Grape seed and grape skin extracts elicit a greater antiplatelet effect when used in combination than when used individually in dogs and humans. The Journal of Nutrition 132 (12): 3592-3598.



MS - 19

Hypoglycemic Mechanism of High Resistant Starch Sago and Red BBBean Flour- Based Analog Rice on Diabetic Rats : a

Nutrigenomics Study

Sri budi Wahjuningsih 1 and Haslina1 1Department of Agricultural Product Technology, Faculty of Agricultural Technology, Semarang University


INTRODUCTION .. Resistant starch is known to be able to decrease blood glucose. One of the products which has rich resistant starch is sago-based analog rice. Previous research has shown that analog rice of sago starch and red bean flour has low IG, and is hypoglycemic. However, how the ypoglycemic mechanism is seen nutrigenomically and its effect on the lipid profile have not been investigated. This study aims to determine the mechanism of hypoglycemic effects and their effect on lipid profiles in diabetic rats. METHODS . Production of sago red bean analog rice and bioassay implementation with experimental animals. Thirty-five male Wistar rats were divided into 5 groups of 7 each and fed different feeds with a standard dietary diet (KS), and four groups of diabetic induction mice with standard dietary feed (DSTD), fragrant mentik rice ( DBMW), sago analog rice (DBS) and sago analog rice with the addition of 10% red bean flour (DBSKM). Lipid profile was observed every week for one month of maintenance, at the beginning and end of maintenance were performed insulin and blood glucose testing for the calculation of the beta HOMA index. Tests were also performed on the levels of the atherogenic index and IHC. At the end of experimental animal preservation, the organ harvesting of the pancreas was conducted to see the histological pancreas. RESULTS AND DISCUSSION. The results showed that sago analog rice (DBS) had the biggest effect of decreasing total cholesterol (86.72%) followed by DBSKM (64.33%) and DBMW (16.35%). The most significant decrease of triglyceride level in DBS group (39.84%) followed by DBSKM (24.44%), and DBMW (9.37%). The lowest HDL increase in DBMW group (3.84%) followed by DBSKM and DBS (14.32%), (23.12%). The most significant decrease of LDL level in DBS group (24.71%) followed by DBSKM and DBMW respectively 16.08% and 6.92%. DBS atherogenic index levels lower than DBSKM, DBMW and DSTD were respectively 1.00, 2.06, 4.75, and 7.41. IHC levels in DBS and DBSKM were 92.42, 85.62 higher than DBMW (85.45) and DSTD (21.97), respectively. Homa B (strength of pancreas beta cells) in sago rice (DBS) on the last week (0.83) was higher than DBSKM (0.46).



MS – 20

Potential Polysaccharide Gel from Durio Zibertinus var.Raja Galuh Rind Extract Towards Klebsiella Pneumoniae Bacteria

Amanah Amanah1,2*, Tiar M Pratamawati 1,2, Mutiara Taslimah3, Risnandya Primanagara1,2

1Departement of Immunology and Tropical disease Faculty of Medicine Universitas Swadaya Gunung Jati 2Department Genetics Faculty of Medicine Universitas Swadaya Gunung Jati

3Student of Faculty of Medicine Universitas Swadaya Gunung Jati *Corresponding author: [email protected]

INTRODUCTION .. Pneumonia disease is commonly caused by Klebsiella pneumonia. Klebsiella pneumoniae a gram negative bacteria, which is prevalent in developing countries. In 2014, from 4,3000,143 toddlers in West Java, 48.06% of them suffered from pneumonia. Especially in Cirebon, 70.9% toddlers were diagnosed with pneumonia. The Rind of Durio zibetinus extract phenolic compounds which are believed to able to inhibit the growth of Klebsiella pneumonia. METHODS . This experimental in vitro study used 12 group samples with an experimental post test only group design, using a liquid dilution method and Mueller Hinton media. The 12 study sample with 3 repetitions consisted of 1 positive control group, 1 negative control group, and 10 treatment groups with the following concentrations: 100%, 50%, 25%, 12.5%, 6.25%, 3.13%, 1.56%, 0.78%, 0.39%, and 0.20%. RESULTS AND DISCUSSION. MIC test results on the tube contain non-motile gram negative bacteria, not encapsulated, large colonies, very mucus and tend to collect. and total plate positive control account showed growth of bacteria number 23,66, group of 100% durian skin durian as much 25,66, group 50% that is 26,00, group 25% 22,67, group 12,5% that is 18,34, group of 6.25% ie 18.00, group 3.13% ie 16.34, group 1.56% ie 13.67, group 0.78% ie 10.67, group 0.39% 8.67, group 0.20% ie 8.34 with statistical analysis p value 0,00 (significant) concentration 0,39% and 0,20% is Minimum Inhibitory Concentration (MIC) bacterium Klebsiella pneumoniae. REFERENCES [1] Ottosen J, Evans H. Pneumonia. Surg Clin North Am [Internet]. 2014;94:1305–17. Available from:

http://www.ncbi.nlm.nih.gov/pubmed/25440125%5Cnhttp://linkinghub.elsevier.com/retrieve/pii/S0039610914001492 Kementrian Kesehatan. Profil Data Kesehatan Indonesia Tahun 2015. Jakarta: Kementerian Kesehatan RI. 2015.

[2] Bott R. Data Kesehatan di Jawa Barat. Igarss 2014. 2014. 1-5 p. [3] Penjumras P, Rahman RBA, Talib RA, Abdan K. Extraction and Characterization of Cellulose from Durian Rind. Agric Agric Sci

Procedia [Internet]. 2014. [4] Ashraf MA, Maah MJ, Yusoff I. 2010. Estimation of Antioxidant Phytochemicals in Four Different Varieties of Durian (Durio

Zibethinus Murray) Fruit. Middle East Journal of Scientific Research 6(5), 465- 471. [5] Faizah LH. Aktivitas antibakteri ekstrak etanol kulit buah durian (Durio zibethinus Murr) terhadap Klebsiella pneumoniae dan

Streptococcus pyogenes serta bioautografinya. 2015. [6] Duazo NO. Crude methanolic extract activity from rinds and seeds of native durian (Durio zibethinus) against Escherichia coli

and Staphylococcus aureus. African J Microbiol Res [Internet]. 2012. [7] Alagesaboopathi C, Kalaiselvi N. 2012. Antimicrobial activities of the root, stem and leaf extracts of Argemone mexicana L.

International Journal of Bioscience 2(5), 61- 68. [8] S. Hokputsaa, A. Gerdditb, S. Pongsamartb, K. Inngjerdingenc, T. Heinzed, A. Koschellad, S.E. Hardinga, B.S. Paulsen, Water-

soluble polysaccharides with pharmaceutical importance from Durian rinds (Durio zibethinus Murr.): isolation, fractionation, characterisation and bioactivity, Carbohydr. Polym. 56 (2004) 471–481.

[9] Pongsamart S, Nanatawanit N, LertchaiponJ, Lipipun V. Novel water soluble antibacterial dressing of durian polysaccharide gel. Acta Hortic 2005; 678: 65–73.



MS – 21

Effects of IGF1-Induced Wharton Jelly Mesenchymal Stem Cells (WJMSCs) Toward Expression of Proinflammatory

Cytokine in Osteoarthritis Model

Ni Luh Wisma Eka Yanti1, Ervi Afifah 1, Wahyu Widowati2*, Rismawati Laila Qodariah1, Fajar Sukma Perdana1, Tjandrawati Mozef3, Ferry Sandra4, Rizal Rizal1, Annisa Amalia1,

Zakiyatul Khoiriyah 1, Indra Bachtiar 5, Harry Murti 5 1Biomolecular and Biomedical Research Center, Aretha Medika Utama, Bandung 40163, West Java, Indonesia

2Medical Research Center, Faculty of Medicine, Maranatha Christian University, Bandung 40164, West Java, Indonesia 3Research Center for Chemistry, Indonesian Institute of Sciences, Serpong, Indonesia

4Department of Biochemistry and Molecular Biology, Faculty of Dentistry, Trisakti University, Jakarta, Indonesia 5Stem Cell and Cancer Institute, Jakarta 13210, Indonesia


INTRODUCTION. Osteoarthritis (OA) which known as degenerative joint disease, is the most prevalent form of arthritis1,2. At the clinical stage of the disease, OA shows an inflammation in the synovial membrane, which tends to develop the synthesis of proinflammatory cytokines, including IL1β and IL-62. Inflammation is the main factor associated with the risk of both progression of cartilage loss and signs symptoms of the disease, including joint pain, swollen, and stiffness, as well as the indicators of synovitis2. Wharton’s Jelly Mesenchymal stem cells (WJMSCs) has been employed as one of important component in tissue engineering for certain disease therapy that is involved in anti-inflammatory events and tissue repair3. METHODS. Insulin-like growth factors 1 (IGF1) is known has a main role in the regulation of immunity and inflammation. The aim of this study is to evaluate the potency of IGF1-induced WJMSCs in stem cell-based OA therapy by analyse the level of proinflammatory cytokines. IGF1-induced WJMSCs (IGF1-WJMSCs) were cocultured with IL1β-induced chondrocyte (IL1β-CHON002) in various ratios (1:2, 1:1, 2:1). The measurements of IL-1β, IL-6, and IL-10 level were performed with ELISA kit method3. RESULTS AND DISCUSSION. The level of IL-1β and IL-6 were decreased in IGF1- WJMSCs and IL1β-CHON002 coculture. In the contrary, the level of IL-10 was increased in IGF1-WJMSCs and IL1β-CHON002 coculture. Based on the results, IGF1-induced WJMSCs is capable to reduce the inflammation as indicated by the decreasing of proinflammatory cytokines concentration and increasing anti-inflammatory cytokines which can be further used as the promising agent in OA therapy. REFERENCE [1] Felson DT, Lawrence RC, Dieppe PA, Hirsch R, Helmick CG, Jordan JM, et al. Osteoarthritis: New Insights. Part 1: The Disease

and Its Risk Factors. Ann Intern Med. 2000; 17: p. 635-646. [2] Sellam J, Berenbaum F. The Role of Synovitis in Pathophysiology and Clinic Symptoms of Osteoarthritis. Nat Rev Rheumatol.

2010; 6: p. 625-635. [3] Seo, Na K. Mesenchymal stem cell-based tissue engineering for chondrogenesis. J Biomed Biotechnol. 2011;: p. 1–8.



MS-22

Progress Report on the Rapid Test Kit Development for Early Detection of Systemic Lupus Erythematosus in Indonesia

Kusworini Handono1*, Krist Nathania Benita 2, Fitria Ummu Habibah3 and Elvira Sari Dewi4

1 Department of Clinical Pathology, Faculty of Medicine, Brawijaya University, Malang, Indonesia. email: [email protected]

2 Medical Doctor Study Program, Faculty of Medicine, Brawijaya University, Malang, Indonesia. email: [email protected] 3 Medical Doctor Study Program, Faculty of Medicine, Brawijaya University, Malang, Indonesia. email: [email protected]

4 Department of Nursing, Faculty of Medicine, Brawijaya University, Malang, Indonesia. email: [email protected]

INTRODUCTION. Gold standard diagnosis for Systemic Lupus Erythemathosus (SLE) is ANA and anti-dsDNA antibody test using ELISA. This method has been used for years, but hardly applicable in rural hospital with no ELISA reader equipment. We are developing rapid test kit for early detection of SLE which is affordable and ease by blotting antigen into membrane. This research has been held since 2015, yet the previous trial did not produce color difference between positive and negative. Our recent trials aim to optimize antigen isolation and its attachment to membrane. METHODS . This was trial-and-error experiment using healthy unused human transfusion blood and manual (non-kit) isolation methods. Lymphocyte was isolated by comparing two substances use of Ficoll and Ery-Lysis Buffer (EB). Nuclear Antigen was isolated by comparing 2 methods and 2 types of eppendorf. dsDNA was isolated by original versus modified salting out method. We compared antigen attachment to nitrocellulose, and PVDF membrane. RESULTS AND DISCUSSION. Lymphocyte was harvested more using single sample human transfusion blood compared to pool multiple sample. The quantity of lymphocyte pellet was almost comparable through isolation using Ficoll versus EB. By comparing 2 methods, more protein yield was achieved through the use of Nuclear Extract (NE) and Cytoplasmic Extract (Buffer). From 15cc of whole blood, can be produced 3-5ul/ul protein. Modified salting out method using high concentration salt and extra 0,05mg/ml Proteinase-K result in more yield, 700-800ug/ul of dsDNA. Attaching dsDNA was feasible using PVDF membrane, rather than Nitrocellulose membrane, by applying 200ug antigen in each blot and BSA 3% blocking rather than milk. Unfortunately the color darkness resulted was not consistent in repeated experiment and fades after the membrane dries. Nuclear protein did not show color difference after attached to nitrocellulose nor PVDF membrane. We suspect too limited antigen was applied to the membrane. CONCLUSION. The cost of producing this kit has been suppressed by using manual technique. There are multiple factors that needed to be optimized in further research, such as sample storage and isolation method to produce more protein yield, also modify DNA-antibody-substrate binding so color change can stay in longer period of time.

Figure 1. A) Blotting nuclear antigen in Nitrocellulose membrane. B)Dot blotting of ds-DNA antigens in nitrocellulose membrane. The dot blotting did not show the difference color between control, positive, and negative.

REFERENCE [1] Holman, Halsted R. "Partial purification and characterization of an extractible nuclear antigen which reacts with SLE

sera." Annals of the New York Academy of Sciences 124, no. 2: 800-806. (1965) [2] Luo, Yuqian, Takeshi Hara, Yuko Ishido, Aya Yoshihara, Kenzaburo Oda, Masahiko Makino, Norihisa Ishii, Naoki Hiroi, and Koichi Suzuki. "Rapid preparation of high-purity nuclear proteins from a small number of cultured cells for use in electrophoretic mobility shift assays." BMC immunology 15, no. 1: 586. (2014)

A B



>>THIS LINE WITH YOUR PAPER IDENTIFICATION NUMBER MS-23

Protein Profiling of Coloring Rice (Oryza sativa L.) using SDS-PAGE and Experiontm260 Analysis

Dewi R. T. Sari

1,2, James R. Ketudat Cairns

3, Anna Safitri

1,4, Fatchiyah Fatchiyah

1,2*

1Research Center of Smart Molecule of Natural Genetics Resources, Brawijaya University, Indonesia.

2Biology Department, Faculty of Mathematics and Natural Sciences, Brawijaya University, Indonesia. 3

School of Chemistry, Institute of Science, Suranaree University of Technology, Nakhon Ratchasima, Thailand

4Department of Chemistry, Faculty of Mathematics and Natural Sciences,

Brawijaya University, Indonesia *Correspondence: [email protected] INTRODUCTION. Black rice is one of coloring rice and contains high nutrients, minerals, and bioactive compounds

1. Black rice becomes more popular due to their benefit such as anti-inflammation, antioxidant,

improving LDL via increasing HDL levels, anti-hyperlipidemic, anticancer, and anti-obesity2,3,4

. This study provides to compare the protein profile of rice by using SDS PAGE and ExperionTM260. METHODS Samples were collected from East Java, Central Java and West Java, there were white rice (WREJ), red rice (RREJ), black rice from East Java (BREJ), Central Java (BRCJ) and West Java (BRWJ). Proteins were extracted from rice seeds and run with SDS-PAGE and ExperionTM260. The protein bands were detected by Coomassie Brilliant Blue (CBB) stain on the SDS PAGE. The molecular weight of the protein was determined by the Rf value. RESULTS AND DISCUSSION. Based on SDS PAGE analyses, fifteen protein bands were detected on each samples whereas based on ExperionTM260, the protein bands in each sample were different (Figure 1). The range of proteins detected on the SDS page was 8-137kDa, whereas ExperionTM260 detected protein bands from 1.2kDa to 260kDa. Several protein has been found in WREJ, RREJ, and BRWJ both on SDS PAGE and ExperionTM260. There were one protein in WREJ (127kDa), two proteins in RREJ (132kDa18kDa) and two proteins in BRWJ (25kDa &21kDa). Whereas the other proteins were not the same in each sample in both analyzes. Several proteins with MW more than 137kDa and less than 8kDa were identified using ExperionTM260, not on SDS-PAGE with CBB stain analyses. CONCLUSION . Our study proved that several proteins bands were detected on ExperionTM260 analyses, but based on SDS-PAGE analyses were not detected as ExperionTM260 analyses.

Figure 1. The Number of protein band which detected on ExperionTM260 and SDS-PAGE REFERENCE [1] Goufo P, Trindade H. 2014. Rice antioxidants: phenolic acids, flavonoids, anthocyanins, proanthocyanidins, tocopherols, tocotrienols, γ-oryzanol, and phytic acid. Food Sci Nutr, 2, 75-104. [2] Ichikawa H, Ichiyanagi T, Xu B, Yoshii Y, Nakajima M, Konishi T. 2001. Antioxidant activity of anthocyanin extract from purple black rice. J Med Food 4(4):211–218. [3] Xia, X., W. Ling, J. Ma., M., Xia, M. Hou, Q., Wang, H., Zhu & Z., Tang. 2006. An anthocyanin rich extract from black rice enhances atherosclerotic plaque stabilization in apoliprotein e deficienr mice. J. Nutr. 136: 2220–2225. [4] Esther T.C., A B.Santhakumara, J L. Christopher, L. Blancharda. 2018. Therapeutic potential of rice-derived polyphenols on obesity-related oxidative stress and inflammation. Journal of Applied Biomedicine. https://doi.org/10.1016/j.jab.2018.03.001.



MS – 24

Comparison of Sport Massage And Combination of Cold Water Immersion With Sport Massage on Decrease of Blood

Lactic Acid Level

Nurul F. Romadhona1 , Gadis M. Sari2 , Dwikora N. Utomo 3

1Department of Sport Health Science, Airlangga University, Surabaya, [email protected] 2Department of Sport Health Science, Airlangga University, Surabaya, [email protected]

3Department of Orthopedics and Traumatology, Dr. Soetomo Hospital, Surabaya, [email protected]

*Corresponding author: [email protected], [email protected]

INTRODUCTION .. Recovery is an attempt to reduce blood lactic acid levels and make the body recover after physical exercise. There are several methods to achieve recovery such as sports massage and cold water immersion (15 oC) combined with sport massage. The effect of cold water immersion (15 oC) and sport massage combined on decreasing blood lactic acid level is still unknown. METHODS . This research method is experimental laboratory with randomized pretest-post test control group design, the number of male subject was 16, each of whom is a member of Surabaya Community Runner. The subjects were randomly assigned to 2 groups, K1 group for recovery sport massage and K2 recovery group for combination of cold water immersion (15 oC) with sport massage. Both groups performed sub-maximal physical exercise by doing fast walk on the treadmill to reach (80% HRM) and then maintained for 5 minutes, followed by recovery process. lactate acid level data was acquired for 3 different times of retrieval which were before physical activity, 5 minutes after sub-maximal physical activity, and immediately after recovery. RESULTS AND DISCUSSION. ANOVA test of lactic acid level post treatment was p = 0,00 (p <0,05) which shows there was significant difference in the decrease of lactic acid level. The paired t-test of each group showed significant result in sports massage group (p = 0,002), and combination cold water immersion (15oC) with sport massage group (p = 0,000). Recovery by soaking in contrasting waters, which result in alternating vasoconstriction and vasodilation, are considered to act in a manner similar to muscle pumping (vaso-pumping) thus improving blood flow and removal of metabolites therefore enhancing recovery. REFERENCE [1] Best, T. M., R. Hunter, A. Wilcox and F. Haq. (2008). Effectiveness of sports massage for recovery of skeletal muscle from

strenuous exercise. Clinical Journal of Sport Medicine 18(5): 446 [2] Gillian EW dan Greg DW, 2013. Cold-water immersion and other forms of cryotherapy: physiological changes potentially

affecting recovery from high-intensity exercise. Extreme Physiology Medicine. 2013; 2: 26. doi: 10.1186/2046-7648-2-26 [3] Peiffer, Jeremiah J. , Abbiss, Chris R. , Watson, Greig , Nosaka, Ken and Laursen, Paul B. 2009. Effect of cold-water immersion

duration on body temperature and muscle function. Journal of Sports Sciences, 27: 10, 987 — 993 DOI: 10.1080/02640410903207424

[4] Versey NG, Halson SL, Dawson BT. (2013). Water immersion recovery for athletes: effect on exercise performance and practical recommendations. Sports Med. 2013 Nov;43(11):1101-30

[5] Wiltshire E. Victoria, Poitras V, Pak M, Hong T, Rayner J, and Tschakovsky ME. 2010. Massage Impairs Postexercise Muscle Blood Flow and ‘‘Lactic Acid’’ Removal: Journal of the American College of Sports Medicine



MS – 25

The Effect of Methanol Extract of Sansevieria Trifasciata Leaves on Pancreatic Beta-Cells Function T1DM and T2DM

Rats

N.Qomariyah1,2* , M.M.Calon, J.K Kruit 3 , J.E. Bruggink, and G. van Dijk1*

1Department of Neurobiology, GELIFES Institute, University of Groningen, The Netherlands; [email protected]

2Department of Biology, Faculty of Mathematics and natural sciences, the State University of Surabaya, Indonesia ([email protected])

3Department of Paediatrics, University Medical Center Groningen, University of Groningen, the Netherlands ([email protected])

*Corresponding author: : [email protected] and [email protected]

INTRODUCTION .. Sansevieria is a genus of about 70 species of flowering plants, native to africa, madagascar and southern asia. It is known to be rich in antioxidants and bioactive compounds, which are presumed to be able to affect pancreatic beta cell function. For this reason, sansevieria is widely used by local communities as an alternative medicine to treat diabetes. The aim of this study is to investigate the efficacy of methanol extract of sansevieria trifasciata leaves on the percentage of islet of Langerhans and glucose homeostasis in rats with type 1 diabetes (T1DM) and type 2 diabetes (T2DM). METHODS . Three months old wistar rats underwent surgical implantation of jugular vein and stomach catheters for repeated blood sampling and intragastrical infusions, respectively. After recovery, they were subdivided into T1DM (45 mg/kg STZ, low fat diet) and T2DM (30 mg/kg, high fat diet) groups. Sansevieria extract was given intragastrically in a dose of 200 mg/kg bodyweight for 25 days. Blood glucose homeostasis was assessed with gastric and intravenous glucose tolerance tests. Pancreas was embedded with paraffin method, the section cutted in 5µm and stained with DAB insulin staining. The percentage of beta-cells was analyzed and calculated using Imagescope program. RESULTS AND DISCUSSION. The blood glucose levels were significantly (p<0.05) decreased in T2DM rats that were treated with sansevieria (compared to the watertreated T2DM group). However, the percentage of beta-cells did not significantly difference in T1DM and T2DM rats.

REFERENCE [1] Srinivasan, K., Viswanad, B., Asrat, L., Kaul, C. L., & Ramarao, P. Pharmacological research, 52(4), 313-320 (2005) [2] Matheus, V. A., Monteiro, L. C. S., Oliveira, R. B., Maschio, D. A., & Collares-Buzato, C. B. Experimental Biology and

Medicine, 242(12), 1214-1226 (2017).



MS – 26

The Morphological Endogenous Development Of Eimeria Necatrix At Primary And Secondary Infected Chickens

And Its Histopathological Effect

Muchammad Yunus1*, Endang Suprihati2 and Agus Wijaya3 1, 2 Department of Veterinary Parasitology, Universitas Airlangga 3 Department of Veterinary Clinic, Bogor Agricultural University


INTRODUCTION. Coccidiosis in chickens is a parasitic disease with great economic significance, which has been controlled successfully for decades using mainly anticoccidial products. To overcome this condition, the choice to approach vaccine use in controlling a more intensive and planned disease is needed. METHODS . Twenty five broilers at three weeks old were divided into two groups. Group 1 was 10 chickens administered aquades, after 14 days then challenged 5 x 103 E. necatrix oocysts, Group 2 was 15 chickens inoculated 5 x 102 E. necatrix oocysts, 4 days post inoculation 5 chickens were sacrificed to observe morphological endogenous development of parasites by histopathological changes examination for primary infection, while oocysts production was calculated on 10 other chickens from day 6 to 12 post primary inoculation, 14 days post primary inoculation then challenged 5 x 103 E. necatrix oocysts, at 4 days post secondary infection, 5 chickens were sacrificed to observe morphological endogenous development of parasites by histopathological changes examination for secondary infection, while oocyst production calculation was done at 6 to 12 days post challenged on other chickens for secondary infection. RESULTS AND DISCUSSION. The inherent difference in reproductive potential is high for E. tenella and E. acervulina, and low for E. maxima. Immunity, which is specific to each coccidian species, results in decreased production of oocysts after ingestion of infective oocysts [7]. CONCLUSION . We concluded that the low doses of virulent E. necatrix had low propagative level and few histopathological changes effect that represents a promising strategy to prevent chickens coccidiosis particularly E. necatrix.

Figure 3. Histopathological changes of mid small intestine by E. necatrix oocyst infected chickens. In primary infection, (G1), there was no

endogenous development stage of parasite due to only water administered, the same condition occurred at E. necatrix in low doses infected chickens (G2), arrow, which parasite appeared clearly well development in the primary infection, although few proliferation and multiplication of E.necatrix in epithelial cell of small intestine. In secondary infection, (G1) , erosion of surface and damage of epithelial cell of mid small intestine due to development of E.necatrix continuously, arrow, propagation of E. necatrix in site infection, erosion of mucosa surface of mid small intestine and be accompanied many parasites proliferation, arrow. On G2 chicken infection, a few endogenous development of parasites due to the presence of protective immunity by induction of primary infection, arrow. Magnification x400.

REFERENCES [1] Yun, C.H., Lillehoj, H.S. and Lillehoj, E.P., 2000, Develop. Comp. Immunol., 24, 303-24. [2] Wallach, M., Pillemer, G., Yarus, S., Halabi, A,, Pugatsch, T. and Mencher, D., 1990, Infect. Immun., 58, 557- 62. [3] Wallach, M., Smith, N.C., Petracca, M., Miller, C.M., Eckert, J. and Braun, R., 1995, Vaccine, 13, 347-54. [4] Jenkins, M.C., Augustine, P.C., Danforth, H.D. and Barta, J.R., 1991, Infec. Immun., 59, 4042- 8. [5] Chapman, H. D. and Cherry, T. E., 1997, J. Appl. Poult. Res., 6 (3), 274–8 [6] Oikawa, H., Kawaguchi, H., Nakamoto, K. and Tsunoda, K., 1975, Nihon Juigaku Zasshi, 37(3), 271-9. [7] Arabkhazaeli, F., Nabian, S., Modirsaneii, M., Mansoori, V. and Rahbari, S., 2011, Iran J. Parasitol., 6 (4), 23–32.



MS – 27

The Aquatic Environmental Quality of Koi Fish (Cypr inus carpio) Pond Infected by Myxobolus sp. Based on the

Biological Status of the Phytoplankton

Uun Yanuhar1*, Nico Rahman Caesar1, Feri Setiawan1, Muhammad Sumsanto1, Muhammad Musa1, Dyah Kinasih Wuragil2

1 Laboratory of Biotechnology and Waters Science, Faculty of Fisheries, and Marine Sciences, Brawijaya University, Jl. Veteran, Malang –Jawa Timur, Indonesia, 65144

2 Laboratory of Animal Diseases Diagnostic, Faculty of Animals Health, Brawijaya University Jl. Veteran, Malang –Jawa Timur, Indonesia, 65144

*Corresponding author: [email protected]; [email protected]

INTRODUCTION. Myxobolus sp. is a type of parasite that causes myxosporeasis. This disease is caused by Phylum Myxozoa, which is the dominant obligate cellular parasite in the waters. Myxobolus sp. attacks a lot of freshwater aquatic species such as koi fish Cyprinus carpio. The status of freshwater waters as a medium for cultured C.carpio related to the presence of phytoplankton and also the occurrence of myxobolus infection in these waters have not been widely revealed. The purpose of this study was to observe the biology of phytoplankton found in the waters pond of C.carpio and the Myxobolus sp. infected C.carpio in Blitar, Indonesia.. METHODS . The research method was a survey of the culture condition, including the analysis of the sample of phytoplankton in water pond, identification of phytoplankton in the targeted fish organs infected with myxobolus sp. C.carpio samples have a length of 1-3 cm and weight of 5-8 grams. Observation of both phytoplankton and myxobolus samples was done using the Olympus CX31 microscopes and Olympus E330-ADU1.2X cameras. RESULTS AND DISCUSSION. The identification results showed that the phytoplankton was from the Charophyta division, genus Mougeotia, Division of Chlorophyta, genus Pediastrum, and Protosiphon, Division of Cyanobacteria, genus Mycrocystis. In Myxobolus sp. infected koi, the gills, the stomach, and the intestine indicated phytoplankton. The phytoplankton found in water cultured pond of koi fish allegedly biologically became an intermediary for infection in koi fish. It is necessary to prove through cellular and molecular methods whether the phytoplankton also contained myxobolus sp.

REFERENCE [1] Nugroho, A. Bioindikator Kualitas Air. Universitas Trisakti. Jakarta, (2006) [2] Zulfiandi, M. Zainuri, and I. Widowati, Pros. S emin. Nas. Kelaut. IX, Hang Tuah Surabaya, pp. 24–31, (2014



MS – 28 The Efficacy of Goat Milk Yogurt for Hypercholesterolemic

rats (Rattus norvegicus) models

Dyah Kinasih Wuragil1,3*, Masdiana Chendrakasih Padaga1, Aulanni’am Aulanni’am 1,2,3 and Chanif Mahdi2*

1Faculty of Veterinary Medicine, Universitas Brawijaya, INDONESIA 2Department of Chemistry, Faculty of Sciences, Universitas Brawijaya, INDONESIA

3Research Group of Biopeptide, Immnodetection and Vaccines (BIODET-Vaccines), Universitas Brawijaya, INDONESIA

*Corresponding authors: [email protected], [email protected]

INTRODUCTION : Goat milk yogurt is known as one of protein source which have more advantages than cow’s milk. Peoples believe that this product have ability to be used as therapeutic agents for many diseases such as Hypercholesterolemia. Hypercholesterolemia is a condition when the cholesterol level in the blood is higher than normal and leads to cardiovascular diseases. Goat milk yogurt has bioactive peptide and bile salt hydrolases which have potency as anti-hypercholesterolemia. on several organs were aortic, heart and kidney based on histopathology and cytokine expression. METHODS : Male rat (Rattus norvegicus) Wistar strain were used in this research which divided into control (A), hypercholesterolemic rat (B), and hypercholesterolemic rats with goat milk yogurt therapy dose of 300 mg/kgBW (C), 600 mg/kgBW (D), and 900 mg/kgBW (E). Hypercholesterolemia were induced by hypercholesterol diet contain of 10% lard, 0.1% cholic acid and 5% boiled quail egg yolk for 14 days. Goat milk yogurt therapies were administered for 28 days. RESULTS AND DISCUSSIONS : This findings showed that goat milk yogurt could repair histopathology of aortic, heart and kidney which was damaged due to hypercholesterol diet. It was also shown declining of TNFα expression on those organs. The higher therapeutic dose, the lower of TNFα expression obtained. CONCLUSIONS : This research concluded that gout milk yogurt has potency to be used as therapeutic for hypercholesterolemia REFERENCES [1] Korhonen, H., and Pihlanto-Leppala, A. 2003. Current Pharmaceutical Design, 9:1297-1308. [2] Zhong,Y., and Z. Heping.2013. The Creative Common Attribution Liceanse: Amerika.



MS – 29

Chondrocyte Pericellular Matrix Strain Fields Of Ar ticular Cartilage Surface In Hyperglicemia Model Of Rat: Cellular

Morphological Study

Ibrahim Njoto 1, Ayly Soekanto1, Ernawati2, Abdurrachman3, Handono Kalim4, Kusworini Handono5, Djoko W. Soeatmadji4, Fatchiyah Fatchiyah6

1 Anatomy Department Faculty of Medicine, Wijaya Kusuma Surabaya University 2Farmacology Department Faculty of Medicine, Wijaya Kusuma Surabaya University

3Anatomy Department Faculty of Medicine, Airlangga University, Surabaya 4Internal Medicine Department Faculty of Medicine, Brawijaya University, Malang 5Clinical Patology Department Faculty of Medicine, Brawijaya University, Malang

6Research Centre of Smart Molecule of Natural Genetics Resources, Faculty of Mathematics and Natural Science, Brawijaya University, Malang


INTRODUCTION. Chondrocytes as one and the only cell resident in atricular cartilage, it products many proteins, molecules, factors. Interaction between chondrocytes and micro enviroment which contain of macromolecules, micromolecules come from internal and external influence of cartilage. Internal influence of cartilage were perform between chondrocytes and it products. One of the chondrocyte product was perlecan (HSPG2) which act as chondrocytes mechanotransducer. External influence of cartilage, such as: hyperglicemia was entering joint capsule and impact to chondrocytes and cartilage micro enviroment. Hyperglicemia caused modification of perlecan molecule through glycation process. It condition was detected as changing of chondrocytes pericellular matrix strain fields, because perlecan was dominantly located at pericellular matrix than extracellular matrix. The aim of this study was to show cellular morphogical changing of chondrocytes pericellular matrix by influence of hyperglycemia. METHODS . Tweenty adults male rats were divided into four groups: control, standard diet, hyperglicemia diet 1, hyperglicemia diet 2; maintained for two month. Then animal model were sacrificed and left knee was taken to observe changing of chondrocytes pericellular matrix strain fields by Scanning Electron Microscope (SEM), at femoral condylus cartilage. The observation area were each of highest central surface of femoral condylus lateral and medial for each sample. Changing of chondrocytes pericellular matrix strain fields were more white light strain fields at group control and I, than group II and III which darker strain fields. This changing of pericellular matrix strain fields was corresponding to changing of perlecan which located at pericellular matrix of chondrocytes in hyperglicemia condition. RESULTS. Result of this research as based to next research for accomplish of hyperglicemia influence to perlecan articular cartilage changing, to prevent degeneration of cartilage towards osteoarthritis. Present study conclude that hyperglycemia influence to chondrocyte pericellular matrix strain fields changing. REFERENCES [1] Lessons From Conditional Knockout Mice. American College of Rheumatology. ARTHRITIS & RHEUMATISM Vol. 64, No.

12: 3835-38. [2] Wilusz R.E., Defrate L.E., Guilak F. 2012. A Biomechanical Role for Perlecan in the Pericellular Matrix of Articular Cartilage.

Abstract Matrix Biol. 31(6): 320-327. [3] Sakai N., Hashimoto C., Yarimitsu S., Sawae Y., Komori M., Murakami. 2016. A Functional Effect of The Superficial

Mechanical Properties of Articular Cartilage as a Load Bearing System in Sliding Condition. Biosurface and Biotribology 2: 26-39.

[4] Kaab M.J., Richards R.G., Ito K., ap Gwynn I., Notzli H.P. 2003. Deformation of Chondrocytes in Articular Cartilage under Compressive Load: A Morphological Study. Cells Tissues Organs 175: 133-139.

[5] Chaterine A., Vorgl-Willis., Iris J., Edwards. 2004. High-glucose-induced structural changes in the heparan sulphate proteoglycan, perlecan, of cultured human aortic endothelial cells. Elsevier Science Direct: Biochimica et Biophysica Acta 1672: 36-45.

[6] Salinas D., Minor C.A., Carlson R.P., Mc.Curtchen C.N., Mumey B.M., June R.K. 2017. Combining Targeted Metabolomic Data with a Model of Glucose Metabolism: Toward Progress in Chondrocyte Mechanotransduction. PLOS ONE 12(1):e0168326.

[7] Kouri J.B., Jimenez S.A., Quintero M., Chicos. Ultrastructural Study of Chondrocytes from Fibrillated and non-Fibrillated Human Osteoarthritic cartilage. Osteoarthritis and Cartilage 4: 111-125.



MS – 30

Anti-inflammatory Effect of Ethyl Acetate Fraction of Galing Plant Extract (Cayratia trifolia) on Male Wistar Rats Induced

by Carrageenan

Djoko Santoso1*, I Ketut Sudiana

2, Anny Setijo Rahayu

3 and Muchammad Yunus

4 1. Department of Internal Medicine, Faculty of Medicine, Universitas Airlangga

2, 3

Department of Anatomic Pathology, Faculty of Medicine, Universitas Airlangga & Department of Veterinary Parasitology, Faculty of Veterinary Medicine, Universitas

Airlangga *Corresponding author: ([email protected])

INTRODUCTION. Traditional medicine is a mixture of unrefined natural ingredients comes from plants or animals used for treatment traditional [1]. This study aims to determine the anti-inflammatory activity of the ethyl acetate fraction of galing plant extract (Cayratia trifolia) on one of the inflammatory parameters and determine the effective dose of ethyl acetate fraction of galing plant extract as anti-inflammatory. METHODS . This study used 25 male wistar rats divided into 5 groups. Group 1 (negative control) Na CMC 0.5%, group 2 (positive control) diclofenac sodium 0.0065 mg /gBW rats, and group 3, 4 and 5 suspension ethyl acetate fractions of galing plant extract 0.0065; 0.013 and 0.026 mg / gBB rats. Inflammation in rats by inducing 1% carrageenan as much as 0.10 ml. The volume of edema every hour is known from the difference in foot volume at certain hours with normal foot volume. The AUC value of the edema volume was calculated by trapezoid method every one hour and calculated for anti-inflammatory power (IP). The AUC value of the edema volume obtained was analyzed statistically by the Kruskal-Wallis and Mann-Whitney tests using SPSS release 16. RESULTS AND DISCUSSION. The galing plant obtained is processed into simplicia on all parts of plant. Simplicia is pollinated and sieved with No. 30/40 sieve. Simplicia what is used is simplicia which passes on No. 30 sieve and does not pass on the sieve no.40. Galing ethyl acetate fraction can have anti-inflammatory effects allegedly caused by secondary metabolites contained therein, namely flavonoids, saponins and steroids. Some flavonoids play a role in inhibiting lipoxygenase while other flavonoids play a role in inhibiting prostaglandin synthesis. The anti-inflammatory mechanism most likely is suspected saponins capable of interacting with many lipid membranes such as phospholipids which are precursors of prostaglandins and other inflammatory mediators. Steroids in the body can inhibit the enzyme phospholipase A2 which is the enzyme responsible for the release of arachidonic acid which is then metabolized by the enzyme cyclooxygenase and lipo-oxygenase which will then release inflammatory mediators. CONCLUSION . Ethyl acetate fraction of Galing (Cayratia trifolia) extract can have anti-inflammatory effects on male white rats induced by caragenine with an effective dose of 0.0065 mg / gBW. REFERENCES [1] Tjokronegoro, A. and Baziat, A. 1992. Fakultas Kedokteran UI. Jakarta. [2] Chattopadyay, Deprasad, Arunachalam, Ghosh, L., Rajendran, K., Mandal, A.B., Bhatta Charya, S.K. 2005. J. Pharm

Pharmaceut Sci 8 (3): 558-564. www.cspsCanada.org. [3] Robbins SL, Kumar V. 1995. Buku Ajar Patologi I. Terjemahan Staf Pengajar Laboratorium Patologi Fakultas Kedokteran

UNAIR , cetakan 2. Jakarta : EGC. [3] Wirakusumah, E.S. 2002. Buah dan sayur untuk terapu. Jakarta : Penebar Swadaya

[4] Nijveldt, R. J., E. van Nood, D.E.C. van Hoorn, P.G. Boelens, K. van Norren, P.A.M. van Leeuwen. 2001. Flavonoids: a

review of probable mechanisms of action and potential applications. American Journal of Clinical and Nutrition 74: 418-425. [5] Nutritional Therapeutics. 2003. NT Factor: Phosphoglycolipids-High Energy Potential. www.propax.com/FAQ/

soy_high_energy.html [2 Desember 2005]. [6] Katzung, B.G. 2002. Farmakologi Dasar dan Klinik. Jakarta: Salemba Medika



MS – 31

Activity of Combination Persea americana Mill and Orthosiphon aristatus (Blume) Miq . Benth Extract in

dissolving Calcium Oxalate by In Vitro

Rini Madyastuti 1, Ietje Wientarsih1, Setyo Widodo1, Erni H Purwaningsih2, Eva Harlina1

1 Department of Clinic, Reproduction and Pathology, Faculty of Veterinary Medicine, Bogor Agricultural University, Indonesia.

2 Department of Medical Pharmacy, Faculty of Medicine, Universitas Indonesia, Indonesia


INTRODUCTION Kidney stone or urolithiasis is presence of one or more calculi in urinary system. It is a disease with high recurrence both in human and small animal. Calcium oxalate is a major component of urolithiasis. The aim of this study is to evaluate the activity of combination extract Persea americana Mill and Orthosiphon aristatus (Blume)Miq. in dissolving calcium oxalate by In Vitro. METHODS The varian combination was compared with commercial brand (group K(+)) using artificial urine as solvent. This experiment used 11 group with varian combination Persea americana and Orthosiphon aristatus (Blume)Miq extract. The measurement of calcium from the solution was used spectrophotometric atomic absorption (AAS). RESULTS AND DISCUSSION. The result showed that combination of extract Persea americana Mill. and Orthosiphon aristatus was effectively in dissolving calcium oxalate. From the data, the combination of the extract at rasio (1:4) (P8) and (4:1) (P9) had the potency as the inhibitor urolithiasis.

REFERENCE [1] Wientarsih I, Madyastuti R, Prasetyo BF, Aldobrata A. 2012. Anti lithiasis of Avocado (Persea Americana Mill.) leaves extract white male rats. Hayati Journal of Bioscience. 19 (1): 49-51. [2] Burns JR. Finlayson B. A proposal for standard reference artificial urine in in vitro urolithiasis experiment. J Urol 1980; 18: 167-

9. [3] Saha S, Verma RJ. 2013. Inhibition of calcium oxalate crystallisation in vitro by an exract of Bergenia ciliate. Arab J Urol. ;11: 187-192



MG – 01

Changes in Endometrial αv β3 Integrin Expression of Macaca nemestrina after Administration of Gonadotropin

dose of 30-70 IU/day in Controlled Ovarian Hyperstimulation Procedure

Nurhuda*, Rachman IA, Muharram R, Siregar NC*, Muji hartini N, Kusmardi,

Pradhita A* *Biomedical Sciences, Faculty of Medicine Universitas Indonesia

INTRODUCTION . Controlled Ovarian Hyperstimulation (COH) procedures adversely affects the endometrial receptivity of the implantation period. These adverse effects were assessed based on changes in αvβ3 integrin expression as a sign of endometrial receptivity. First purpose is to explain changes in αvβ3 integrin expression in endometrium of Macaca nemestrina after ovarian stimulator administration and compare with control. Second is to see the correlation between levels of steroid hormone phase before ovulation or the day of hCG administration with αvβ3 integrin expression. METHODS. Six teen female Macaca nemestrina were devide into 4 groups consisting of one control group and three treatment groups. The treatment grouns that is Gonadotropin (recombinant FSH) dossage, which is: the first dose is 30 IU / day, the seccond dose is 50 IU / day, and the third dose is 70 IU / day. The administering protocol COH regimens was carried out using the long protocol procedures namely long-dose analogue GnRH combine with recombinant FSH doses of 30, 50, and 70 IU / day for 10-12 days of injection. Blood collection is done in the phase before ovulation, which is the peak of estradiol secretion of hCG delivery day. Endometrial retrieval is performed at mid-luteal phase of 8-10 days after the highest peak of estradiol prior to the ovulation phase or the day of hCG administration. Expression of αvβ3 integrins was assessed immunohistochemically and the concentrations of estradiol and progesterone hormones were measured by ELISA technique. RESULTS AND DISCUSSION. Based on Anova test, there was no significant difference between expression of integrin αvβ3 control group and treatment (p = 0.107). Similarly, in the correlation test there was no significant association between αvβ3 integrin expression with estradiol and progesterone hormones on the day of hCG administration. When compared to the level of progesterone secretion day of hCG, there was a decrease of αvβ3 integrin expression in progesterone concentration> 6 ng / mL. CONCLUSION. There was no significant difference of αvβ3 integrin expression between control group and treatment group, 30-70 IU gonadtropin dose. But there are changes in αvβ3 integrin expression occurred at progesterone days of hCG> 6 ng / ml. REFERENCE [1] Dey SK, Lim H, Das SK, Reese J, Paria BC, Daikoku T, Wang H. Molecular cues to implantation. Endocr Rev 2004; 25: 341-

373. [2] Ben-Nun, I., Jaffe, R., Fejgin, M.D., and Beyth, Y. Therapeutic maturation of endometrium in in vitro fertilization and embryo

transfer. Fertil Steril. 1992; 57: 953–962. [3] Fleming R, Jenkins J. The source and implications of progesterone rise during the follicular phase of assisted reproduction cycles.

Reprod Biomed Online. 2010; 21 ( 4): 446– 449. [4] Noyes, R.W., Hertig, A.T., and Rock, J. Dating the endometrial biopsy. Fertil Steril. 1950; 1: 3–25. [5] Lessey BA, Castelbaum AJ, Wolf L, Greene W, Paulson M, Meyer WR, et al. Use of integrins to date the endometrium. Fertil

Steril. 2000;73(4):779–787. [6] Lessey BA, Damjanovich L, Coutifaris C, Castelbaum A, Albelda SM, Buck CA. Integrin adhesion molecules in the human

endometrium. Correlation with the normal and abnormal menstrual cycle. J Clin Invest. 1992;90(1):188–195. [7] Van der Gaast MH, Beier-Hellwig K, Fauser BC, Beier HM, Macklon NS. Endometrial secretion aspiration prior to embryo

transfer does not reduce implantation rates. Reprod Biomed Online 2003; 7: 105-109. [8] Sing H, Aplin JD. Adhesion molecules in endometrial epithelium: tissue integrity and embryo implantation. J Anat.

2009;215(1):3–13. [9] Arici, A., Engin, O., Attar, E., and Olive, D. Modulation of leukemia inhibitory factor gene expression and protein biosynthesis in

human endometrium. J Clin Endocrinol Metab.1995; 80: 1908–1915.



MG – 02

Development of a Functional Assay to Study The Impact of CHD7 Missense Mutations in CHARGE Syndrome

Gara Samara Brajadenta1,2* , Frédéric Bilan1,3 , Brigitte Gilbert-Dussardier1,3 , Alain Kitzis1 ,

Vincent Thoreau1 1EA-3808 Neurovascular Unit and Cognitive Impairments, University of Poitiers, Poitiers, France

2Division of Human Genetics, Faculty of Medicine Swadaya Gunung Jati University, Cirebon, Indonesia 3Department of Genetics, Poitiers University Hospital, Poitiers, France

*Corresponding author: : [email protected]

INTRODUCTION .. CHARGE syndrome (CS; OMIM 214800) is a rare genetic disease with various congenital anomalies including coloboma, heart defect, choanal atresia, retarded growth and development, genital anomalies, and ear anomalies/deafness. The majority of CS cases are caused by nonsense or frameshift mutations in one allele of CHD7 gene. Besides, missense mutations are rarely found. Haploinsufficiency is hypothesized as the pathogenic mechanism causing CS. CHD7 encodes a chromodomain protein, involved in the ATP-dependent remodeling of chromatin. CHD7 protein also plays a role as a positive regulator of the nucleolar expression of the 45S ribosomal RNA precursor. The objective of this study is to develop a relatively simple biological test of the CHD7 protein, to study the impact upon CHD7 protein functionality of CHD7 missense mutations encountered in CHARGE patients. METHODS .

The CHD7 protein, either wild-type or mutated, were overexpressed in HeLa cell line. Expression and localization of CHD7 protein was demonstrated by western blot and immunofluorescence. Regardless of the experimental conditions, we observed a nucleoplasmic and nonnucleolar localization of CHD7 protein, both for wild-type and mutated alleles. To determine the consequences of the expression of CHD7 alleles, we measured the relative transcription levels of 45S rRNA, Sox4, Sox10, Myrf and Id2 genes, whose transcription is regulated by CHD7 according to the literature. We performed qRT-PCR experiments following three independent transfections. The expression of these genes was normalized against the housekeeping gene GAPDH and calculated by the Livak method RESULTS AND DISCUSSION. We observed that the expression of wildtype CHD7 leads to a significant (p <0.001) and reproducible decrease of transcription level for all reporter genes. By contrast, when mutated CHD7 alleles were expressed, the transcription levels of these genes appear identical to that measured in the mock-transfected cells. These results suggest that the three mutated alleles of CHD7 (G1982W, R2319C, L1302P) are not polymorphisms but that the variant proteins are non-functional. Therefore, these mutations can be considered as causal of CHARGE syndrome.



MG – 03

Prospects and Problems the Amplification of Dna Metagenomic From Clove Plant Collected from Plantation

Community in Ternate Island

Nurhasanah1, Sundari2, Nurmaya Papuangan3

1,2,3 Prodi Pendidikan Biologi, Fakultas keguruan dan Ilmu Pendidikan Universitas Khairun Ternate,Indonesia

Corespoding Author: [email protected]; email: [email protected]

INTRODUCTION DNA content in total microorganisms in plants can be utilized for DNA fingerprint studies and a description of the physiological conditions of the plant. The metagenomic technique represents a novel approach in complex genomic analysis of total non-cultured microorganisms. The purpose of this study was to obtain metagenomic DNA isolated from clove plantations of communities in Ternate. METHODS The method used in this research is total DNA isolated from leaf and stem samples directly using ZymoBiomic (Zymo Research DNA Extraction) Kit, then amplified DNA based on 16s RNA with forward sequence 27F (5'-AGAGTTTGATCATGGCTCAG-3 ') sequence and sequence reverse ie 1492R (5'-GGTTACCTTGTTACGACTT-3 '). RESULTS AND DISCUSSION The results of this study show that of 4 samples only 1 sample from fresh leaves well amplified of successful. Provided amplicon size of 1400bp. REFERENCE [1] Kuske, C.R., Barns, S.M., dan Busch, J.D., (1997), Diverse Uncultivated Bacterial Groups from Soils of the Arid Southwestern

United States That Are Present in Many Geographic Regions, Applied and Environmental Microbiology, 0099-2240. 63 (9) : 3614-3621

[2] Sambrook J., Fritsch, E. F., and Maniatis, T., (1989), Molecular Cloning: A Laboratory Manual 2nd Ed, Could Spring Harbour Laboratory Press, USA

[3] Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S. (2011). MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28: 2731-2739



MG – 04

Phylogenetics Relationship of the Genus Cyrtodactylus (Squamata; Gekkonidae) From Java and Sumatra Based on

ND4 Gene

Muhammad Alif Fauzi, Nia Kurniawan

Biology Department, Faculty of Mathematics and Natural Science, University of Brawijaya

Corespoding Author: [email protected]

INTRODUCTION DNA content in total microorganisms in plants can be utilized for DNA fingerprint studies and a description of the physiological conditions of the plant. The metagenomic technique represents a novel approach in complex genomic analysis of total non-cultured microorganisms. The purpose of this study was to obtain metagenomic DNA isolated from clove plantations of communities in Ternate. METHODS The method used in this research is total DNA isolated from leaf and stem samples directly using ZymoBiomic (Zymo Research DNA Extraction) Kit, then amplified DNA based on 16s RNA with forward sequence 27F (5'-AGAGTTTGATCATGGCTCAG-3 ') sequence and sequence reverse ie 1492R (5'-GGTTACCTTGTTACGACTT-3 '). RESULTS AND DISCUSSION The results of this study show that of 4 samples only 1 sample from fresh leaves well amplified of successful. Provided amplicon size of 1400bp. REFERENCE [1] Kuske, C.R., Barns, S.M., dan Busch, J.D., (1997), Diverse Uncultivated Bacterial Groups from Soils of the Arid Southwestern

United States That Are Present in Many Geographic Regions, Applied and Environmental Microbiology, 0099-2240. 63 (9) : 3614-3621

[2] Sambrook J., Fritsch, E. F., and Maniatis, T., (1989), Molecular Cloning: A Laboratory Manual 2nd Ed, Could Spring Harbour Laboratory Press, USA

[3] Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S. (2011). MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28: 2731-2739



BB– 01

Topical Gel Formulation of Sponge Extract Nanoemulsion with Various Concentration of CMCNa

Aida Humaira 1*, Nada Hanifah2, and Yusuf Farid Achmad3

1 Faculty of Pharmacy, Universitas Gadjah Mada Email:[email protected] 2 Faculty of Veterinary Medicine Universitas Gadjah Mada Email: [email protected] 3 Faculty of

3Veterinary Medicine Universitas Gadjah Mada [email protected] *Corresponding author: [email protected]

INTRODUCTION. . Sponge is one of the important source of active substance between avertebrata[1]. Active substances that can isolated from sponge are saponin, tannin, alkaloid, and terpenoid [2]. Nanoemulsion is one of the greatest and advantageous dosage forms with the application of nanotechnology in pharmaceutical formulation [3] .Very small size droplets of the nanoemulsion favor better drug absorption and targetting[4]. METHODS . Preparation of Nanoemulsion and Nanoemulgel The nanoemulsion was prepared using Virgin Coconut Oil (VCO), Tween 80, PEG 400, consecutively as the oil phase, surfactant, and cosurfactant, with the ratio of 1:4:1, stirred until an isotrophic mixture was obtained. The sponge extract was then dissolved into the mixture and lastly the aqueous phase was added. Nanoemulgel was obtained by mixing the prepared nanoemulsion with the gel base under gentle stirring. The gel base was prepared by dissolving CMC Na in hot distilled water, with CMC Na amount varied into 2%, 3%, 4%, and 5% (w/w). Nipagin was added into the gel base as preservative [5]. Lastly, propylene glycol as humectant and glycerine to increase gel viscosity were added into the formed gel base [6]. Nanoemulgel examination Nanoemulgels were observed physically with color, scent, and homogeinity parameters[7]. The acidity levels of nanoemulgels with different amount of CMC Na were observed by measuring the pH [8].Spreadability was tested to ensure even distribution of nanoemulgels when applied on skin. Nanoemulgel was placed between two circular glasses, left on for 1 minute, then weigh was added each minute starting from 50 grams until 200 grams. Gel distribution diameter was measured for each weigh added. RESULTS AND DISCUSSION. Nanoemulgel examination The prepared gel formulation were greenish in CMC-Na 2% and 3%, brownish in CMC-Na 4% and 5%. All the prepared gel formulation were viscous and homogenous. The pH values of all prepared formulations ranged from 6,5-7,0 which is considered acceptable to avoid the risk of irritation upon application to the skin. Spreadability was in the range of 3.94 to 6.43. Maximum spreadability was observed for gel containing CMC-Na 2%. The minimum spreadability test was observed for gel containing CMC-Na 5%. The spreadability of gel suggested is between 3-5 cm [9].

REFERENCE [1] T. Muniasih. Metabolit Sekunder dari Spons Sebagai Bahan Obat-Obatan.Oseana 28(3):27-33 (2003) [2] M.Y. Putra, T.A Hadi. Chemical Composition, Antimicrobial, Cytotoxic, and Antimiplasmodial Activities of Three Sponges from

Buton Islan,Indonesia. Jurnal Ilmu Kelautan. 22(3):147-154. (2017) [3] Sutradhar,B.S.,Amin .L.2013. Nanoemulsions:Incereasing possibilities in drug delivery. Eur.J.nanomed 5(2): 97-100. [4] Mason TJ, Wilking JN, Meleson K, Chang CB, Graves SM. 2006.Nanoemulsions: formation, structure and physical properties.J

Phys-Condens Matter;18:636 – 43. [5] De Villiers, Melgardt. (2009). Antimicrobial Preservatives. 203-2015. [6] Melani, H. D., T. Purwanti, and W. Soeratri. 2005. Korelasi Kadar Propilenglikol dalam Basis Dan Pelepasan Dietilammonium

Diklofenak Dari Basis Gel Carbopol ETD 2020. Majalah Farmasi Airlangga, 5(1): 1-6. [7] Anief, M. 1997. Ilmu Meracik Obat. Gajah Mada University Press, Yogyakarta. [8] Tranggono, R.I., dan F. Latifah. 2007. Buku Pegangan Ilmu Pengetahuan Kosmetik. PT. Gramedia, Jakarta. [9] Garg, A., D. Aggarwal, S. Garg, and A. K. Sigla. 2002. Spreading of Semisolid Formulation: An Update. Pharmaceutical

Tecnology. September: 84-102.



BB– 02

The correlation of Knowledge in Preganant Women and Huband Support with Antenatal Care (ANC) Compliance at

Puskesmas Kersana Brebes

Azwin Abdul J., Ricardi W. Alibsjah, Donny Nauphar, Tiar M. Pratmawati

1 Faculty of Medicine, University Swadaya Gunung Jati INTRODUCTION. Maternal mortality is one of the health issues that continues to cause worldwide concern. Indonesian Ministry of Health recommends a minimum of 4 antenatal care visits during the pregnancy, with a minimum 1 visit in the first trimester, 1 visit in the second trimester and 2 visits in the third trimester. Quality antenatal care is key to reduce maternal mortality during pregnancy, labor, and after-labor. This study aims to find out the effect of knowledge of pregnant mothers and support of husbands with the compliance of antenatal care visits at Puskesmas Kersana Kabupaten Brebes. METHODS. This was a cross sectional observational design involving 70 pregnant women in the third trimester using total sampling. Data were obtained from interview with the pregnant women and records of maternal health control books. Data were analyzed using Spearman’s correlation test and logistic regression. RESULT AND DISCUSSION. At the significant level of 5% significance, knowledge showed r=0.546, p=0.000 and husband support showed r=0.276, p=0.00. Multivariate analysis showed a value of PR=5.267 (CI95%=2.143±12.949) for knowledge and a value of PR=1.514 (CI 95%=0.387±5.922) for husband support. CONCLUSION. Both knowledge of pregnant mothers and husband support has a positive effect on compliance of antenatal care visit. Pregnant mothers must be further educated with the benefits of antenatal care to increase visitation compliance.

REFERENCE 1. UNICEF Indonesia. Kesehatan Ibu & Anak. UNICEF Indonesia [Internet]. 2012; Available from: www.unicef.or.id 2. Kementerian Kesehatan RI. Profil Kesehatan Indonesia 2015 [Internet]. Jakarta: Kemenkes RI; 2016. Available from: http://www.kemkes.go.id 3. Dinkes Jateng. PROFIL KESEHATAN PROVINSI JAWA TENGAH [Internet]. Semarang; 2015. Available from: www.dinkesjatengprov.go.id 4. Badan Litbangkes Kemenkes RI. Riset Kesehatan Dasar 2013 [Internet]. Depkes RI; 2013. Available from: http://www.depkes.go.id 5. WHO. Trends in Maternal Mortality : 1990 to 2015. 2015. 6. Nae SN. Hubungan Pengetahuan Ibu Hamil Trimester III Dengan Frekuensi Kunjungan Antenatal Care. J Ilm Kebidanan. 2015;3(2):47–53.



BB– 03

Google Trend Analysis on Realization Scope of Pekan Imunisasi Nasional 2016

Risnandya Primanagara1*, Shofa Nur Fauzah2, Amanah3, Uswatun Khasanah4

1 Health Informatics Study Group, Medical Faculty of Swadaya Gunung Jati University, [email protected]

2 Public Health Study Group, Medical Faculty of Swadaya Gunung Jati University, [email protected] 3 Biomedic Study Group, Medical Faculty of Swadaya Gunung Jati University, [email protected]

4 Health Informatics Study Group, Medical Faculty of Swadaya Gunung Jati University, [email protected]


INTRODUCTION . Search trend on google was facilitated on the url trends.google.com, allows user to look for trending search on given condition, time and location. Indonesia held National Immunization Week (Pekan Imunisasi Nasional) on 8-15 March 2016. During that period of time, people animo in Indonesia is high, including in google search. This research is conducted to answer whether search trend and realization scope are related for Pekan Imunisasi Nasional. METHODS. Data gathered by visiting the webpage trends.google.com and search for Pekan Imunisasi Nasional located in indonesia during 2016 [1]. Data is compared with the realization scope data from infodatin on Ministry of Health publication [2]. Data separated by province, and empty province data was excluded. Data was analyzed using 2 related Sample test, using Computer Assisted Statistic Analyis RESULTS AND DISCUSSION Data analysis shows that there were significance difference between Google trends value and the Realization Scope number (p=0,01). Thus, Google trends value and the Realization Scope number is related. Despite the result, we found that the animo from indonesian people towards Pekan Imunisasi Nasional is real, showed by monthly results is overwhelming during the period. Furthermore, analysis on google trends result can be observed onto different approach. REFERENCES [1] Google, "trends.google.com," 1 07 2018. [Online]. Available: https://trends.google.com/trends/explore?date=2016-01-01%202016-1231&geo=ID&q=pekan%20imunisasi%20nasional. [2] Kementrian Kesehatan Republik Indonesia, "infodatin Imunisasi 2016," 24 April 2016. [Online]. Available: http://www.depkes.go.id/folder/view/01/structure-publikasi-datapusat-data-dan-informasi.html.



BB– 04

Decomposition Rate of Many Organic Waste Using Innovative Biopore

Winarsih

Surabaya State University, [email protected]

*[email protected]

INTRODUCTION. The present of land in many villages, such as Surabaya, as the place of managing many organic waste is decreasing through the years, and it went became zero land due to the human activities to fulfill their daily needed. Managing household waste became important due to the limit of the land presented as the garbage dump and the increased volume of the waste itself. Resolving this problem can be done by using innovative biopore on the road at the villages. The aim of this research were calculated the decomposition rate of many organic waste that was composted and the quality of nutrients (C/N rasio, N,P,K) included in 4 kind of organic waste compos. METHODS. This research was an experimental research. Sample were collected by using purposive random sampling. Variable of this research were 4 kinds of organic waste including 1) household waste, 2) rind, 3) leaves, and 4) the combination of household waste, rind, and leaves. RESULTS AND DISCUSSION. The result showed that the decomposition rate of rind waste was 0.135; household waste was 0.116; combination waste was 0.109; and leaves waste was 0.071. The nutrient quality of compos included C/N rasio in all waste sample is very high. The level of nutrient N in compos made by combination waste was low, and the other 3 was medium. The level of nutrient P in compos made by combination waste was medium, but the other 3 was high. The level of nutrient K for all kind of waste was medium.

REFERENCES

[1] Dharmawan, IWA, Neviaty P. Zamani , and Hawis H. Madduppa. Journal of Oseanologi dan Limnologi di Indonesia,1-10,1,1 (2016)

[2] Hayati E., A. Halim Ahmad, and Cut Taisir Rahman. Journal of Agrista 14, 1 (2010)



BB– 05

A Biomass (Champo Leaf) Astable Multivibrator memory

circuit

Gaurang Patel1, Dr.S.P. Kosta2, Dr.Jitendra P. Chaudhari3

Charotar University of Science and Technology, Changa, Gujarat, India [1,2,3] *Corresponding author: 1 [email protected],

2 [email protected], 3 [email protected]

INTRODUCTION. Novel new application of live biomass media (green leaves) as electronic circuit components/circuits an electronic memory circuit is presented. Dr.Shivprasad Kosta conceptualized and published development of biomass electrical circuit components (R, C, diode, transistor) in 1995. In live green leaf the xylem layer sap and phloem layer liquid fluid form electrically charged circuit loops (paths). By supplying appropriate Vcc at various points of leaf along a central mid nerve through pinned connecting wires (harness) at appropriate points the electronic circuit components were realised. METHODS. A typical standard theoretical astable multi-vibrator circuit with two identical NPN transistors, four voltage biased registers (R1, R2, R3, and R4) and two coupling capacitors C1 and C2 interconnected by wire harness as shown in Fig1. Figure1 theoretical circuit thus was transformed to biomass based astable multivibrator circuit.Fig.2 depicts actual experimental biomass astable multivibrator in green Champo leaf. RESULTS AND DISCUSSION. Details of input and output signal are depicted in table1. Parameter Input Output Bit stream 01001111 01001111 Voltage 5V 3.7V Frequency 111 KHz 111 KHz Conclusion: Study of table1 depicts desired biomass astable multivibrator electronic behaviour.

REFERENCE [1] S.P.Kosta, Vimal Patel et al.-2003 “Green Biomass bistable multivibrator” International; journal of electronics (Taylor and

Francis, U.K.) vol.90, pp 117-21. 2) [2] S P Kosta, Shakti Kosta et-al 2010 “Novel Electronic device diode made from human blood” International journal of biomedical

engineering and consumer Health informatics vol.2 NO 1 Jan-JUNE PP 7-13.



PT – 01

The Effects of Anti-diabetic Metals (V, Mo, W, and Cr) on Cellular Metabolism of Insulin-Sensitive Cells

Anna Safitri 1,2*，，，，Aviva Levina1

，，，，Peter A. Lay1 1School of Chemistry, the University of Sydney, City Road, Camperdown, 2006, Australia

2 Chemistry Dept., Brawijaya University, Malang, 65145, Indonesia *Corresponding author: [email protected]

INTRODUCTION. The mechanisms of action of V(IV/V), Mo(VI), W(VI), and Cr(III) compounds in this study are thought to be mainly due to their negative regulation in insulin signalling pathway, by inhibition of protein tyrosine phosphates (PTPs) [1], which dephosphorylate phosphotyrosine residues. Therefore, these metal compounds not only affect the insulin signalling pathway but also affect the whole cellular metabolism. METHODS. In the current work, this can be carried out by measuring mitochondrial functions of the live cells in real time using Seahorse Extracellular Flux (XF) analyzer, by calculating oxygen consumption rate (OCR), as an indication for oxidative phosphorylation, and measuring the extracellular acidification rate (ECAR) as an indication for glycolysis, which are related to glucose metabolism and uptake [2]. RESULTS AND DISCUSSION. Results showed that L6 muscle cells treated with V(V/IV), Mo(VI), and W(VI) compounds had significant improvements in their mitochondrial functions, with similar effects to those observed in insulin treatment. Results from the glycolysis assay also show positive effects from insulin and V(V/IV), Mo(VI), and W(VI) complexes on the glycolysis, glycolytic capacity, and glycolytic reserve of L6 muscle cells. The exception was shown from Cr(III) treatment of the cells, which did not seem to stimulate glucose metabolism on L6 cells; nevertheless, it did not show signs of toxicity to the cells. This suggests that Cr(III) did not have insulin-like effects on mitochondrial respiration and glycolysis.

REFERENCE [1] Levina, A.; Lay, P. A. Dalton Trans. 2011, 40, 11675-11686. [2] XF Cell Mito Stress Test Kit, Seahorse Bioscience: North Billerica, MA, USA, 2010.



PT – 02

Conserved Amino Acid Residues of LexA Repressor of ESKAPE Pathogens and its Potential as a Drug Target

Rio Risandiansyah1, Reza Hakim2, Yoni R. Bintari 3

1,2,3 Medical Faculty, Universitas Islam Malang


INTRODUCTION .. Antimicrobial resistance (AMR) in bacteria may be obtained via random mutation resulting from an error-prone DNA repair, controlled by LexA repressors [1]. Discovery drugs which inhibiting LexA repressor cleavage may provide a different method to reduce or delay the development of AMR in pathogens, particularly in ESKAPE pathogens (Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa and Enterobacter sp.). However, it is unknown whether the repressor in these species has similar properties. This study aims firstly to discover conserved amino acids in ESKAPE pathogens compared to E. coli which may indicate functionally important amino acid residues. Secondly, this study screens the ability of several known anti-mutagenic compounds (dihydroquercetin, gallic acid, lutein, and beta-carotene) to bind to LexA repressor. METHODS . Amino acid sequences was collected from NIH database, using a search term of the “species name” and “LexA repressor”. Obtained sequences (536 hits) was then aligned using JALVIEW software (ClustalX) to discover the presence of the catalytic dyad (S-119 and K-156), and known cleavage sites A-84 and G-85[2]. Conserved regions was determined by comparing LexA repressor across all species. Molecular docking was performed using Autodock Vina on compounds (Pubchem) against LexA repressor (PDB ID: 3JSO), and visualized in Biovia Discovery Studio 2017-R2. RESULTS AND DISCUSSION. LexA repressors in ESKAPE pathogens (131/536 hits) were found to be serine protease with unconventional catalytic dyads. Several amino acid residues, mostly in close proximity to the catalytic dyads and cleavage site, was found to be conserved in >80% of hits across all species. From the tested compounds, beta-carotene and lutein (binding affinity was -8.3 and -8.0 kcal/mol, respectively) was found to form alkyl bonds, while dihydroquercetin and gallic acid (-6.9 and -5.0 kcal/mol, respectively) was found to form hydrogen bonds with conserved amino acids. Whilethis binding may indicatesome LexAcleavage interruption, none wasfoundtointeractwiththe catalyticdyads. REFERENCE [1] Giedraitienė, A. et al. (2011) ‘Antibiotic resistance mechanisms of clinically important bacteria.’, Medicina (Kaunas,Lithuania),

47(3), pp.137–46. doi: 1103-01e[pii].

[2] Kovačič, L. etal. (2013) ‘Structural insight into LexA – RecA * interaction’, Nucleic Acids Research, 41(21), pp. 9901–9910. doi: 10.1093/nar/gkt744.



PT – 03

Cryoprotectant Combination Ethylene Glycol and Propanediol on Mice Blastocyst Viability Post Vitrification

Adinda Kresna1, Widjiati 2*, Tita Damayanti3

1Student of Faculty of Veterinary Medicine Universitas Airlangga 2Veterinary Anatomy Departement, Faculty of Veterinary Medicine Universitas Airlangga

3Veterinary Reproduction Departement, Faculty of Veterinary Medicine Universitas Airlangga


INTRODUCTION. Vitrification is the best method to store frozen embryo because it is protecting the embryo from environmental disasters and infectious diseases. All cryoprotectant has a toxic effect that can cause osmotic damage depending on its concentration and duration of exposure. During cryopreservation with vitrification method, there is physicochemical changing due to physiologic stress. Morphological changing like fracture zone, lysis and degeneration of the membrane, and intracellular damage that cannot be seen with a usual microscope. The toxic effect of cryoprotectant can be minimized by giving the right concentration during vitrification process [1]. This research was aimed to find the effectiveness of vitrification using a combination of intracellular cryoprotectant especially EG and PROH in the same stage of the embryo, especially blastocyst. METHODS . This research was using mice embryo in blastocyst stage as the sample. There were 4 treatment groups in this research, Treatment Group 1 (T1) : PBS + EG 30%, Treatment Group 2 (T2): PBS + PROH 30%, Treatment Group 3 (T3): PBS + EG 20% + PROH 10% and Treatment Group 4 (T4): PBS + EG 10% + PROH 20%. RESULTS AND DISCUSSION. The combination could reduce the toxic effect from propanediol with combination ethylene glycol 20%, and the used of propanediol with concentration 10%(T3) could help ethylene glycol molecule more stable during penetration in the cell membranes but still there were blastocyst that became death caused by the osmotic stress from ethylene glycol 20%. While using ethylene glycol 10% and propanediol 20%(T4), showed the viability of blastocyst was 100%. This result it was known that using propanediol with concentration 20% could make the membrane plasma more stable during penetration and warming process, also ethylene glycol with concentration 10% could reduce the toxic effect from propanediol.

Fig 1. The result from the research

REFERENCE [1] Widjiati, Ratri AW, Arifin, MZ. 2011. Pengaruh berbagai konsentrasi krioprotektan propanediol pada proses vitrifikasi

terhadap viabilitas embrio mencit pasca thawing. Veterinaria 4 (2).



PT – 04

Okra Infusion Water Improving SOD and CRP Level on Diabetic Induced Rats

Nurina Tyagita1*, Kurnia Putri Utami 2, Fitrian Hanif Zulkarnain 3, and Azizah Hikma Safitri4

1 Biochemistry Department, Medical Faculty of Sultan Agung Islamic University, Semarang [email protected]

2 Medical Faculty of Sultan Agung Islamic University, Semarang [email protected] 3 Medical Faculty of Sultan Agung Islamic University, Semarang [email protected]

4 Biochemistry Department, Medical Faculty of Sultan Agung Islamic University, Semarang [email protected]


INTRODUCTION. Chronic hyperglycemia on Diabetes Mellitus type 2 progression leads to inflammation and oxidative stress [1], [2]. Combination of inflammation and oxidative stress triggers various complication including macro and microvascular disturbance, and also death [3]. Blood glucose controlling is mandatory to prevent this damages, either using oral hypoglycemic agent [4] or medicinal plant, such as okra. This study investigate the effectiveness of okra infusion water (OIW) in improving CRP and SOD. METHODS. Posttest only control group design was used in this study. Using 24 male Wistar rats, divided randomly into 4 groups: Control, received no induction nor treatment, 3 groups were induced by 65 mg/kg bW streptozotocine and 110 mg/kg BW nicotinamide: STZ, METF, OKRA. After successfully induced, METF & OKRA were given 9 mg metformin and 3.6 ml OIW for 28 days. Blood samples collected at day 40. CRP and SOD tested using ELISA. Data was analyzed using ANOVA and followed by LSD posthoc test (p<0.05). RESULTS AND DISCUSSION. SOD level on OKRA group was much higher than SOD level on STZ group (p<0.05). Other study found that okra reduced ROS level on rats. Quercetin in okra is a potential antioxidant, functioning in quenching ROS, chelating metal ions and inhibit oxidases activity [5]. Decreased ROS production will enhance SOD level, as first line antioxidant [6]. CRP level on OKRA group was lower than those on STZ group (p<0.05). Okra administration was able to attenuate pro-inflammatory cytokines, such as TNF-α and IL-1β. Okra reduced Lipopolysaccharide (LPS) induced NF-kB p65 phosphorylation. Okra also inhibited LPSinduced Akt phosphorylation, which is an upstream molecule of NF-kB [5].

REFERENCE [1] S.-C. Chang and W.-C. V. Yang, “Hyperglycemia, tumorigenesis, and chronic inflammation,” Critical Reviews in Oncology/Hematology, vol.

108, pp. 146–153, Dec. 2016. [2] T. V. Fiorentino, A. Prioletta, P. Zuo, and F. Folli, “Hyperglycemia-induced oxidative stress and its role in diabetes mellitus related cardiovascular

diseases.,” Current pharmaceutical design, vol. 19, no. 32, pp. 5695–703, 2013. [3] U. S. D. of H. and H. S. and U. S. D. of Agriculture, 2015 – 2020 Dietary Guidelines for Americans. 2015. [4] A. B. Olokoba, O. A. Obateru, and L. B. Olokoba, “Type 2 diabetes mellitus: a review of current trends.,” Oman medical journal, vol. 27, no. 4,

pp. 269–73, Jul. 2012. [5] N. Mairuae, P. Cheepsunthorn, C. L. Cheepsunthorn, and W. Tongjaroenbuangam, “Okra (Abelmoschus esculentus Linn) inhibits

lipopolysaccharide-induced inflammatory mediators in BV2 microglial cells,” Trop J Pharm Res Tropical Journal of Pharmaceutical Research Journal Citation ReportsScience Edition, vol. 16, no. 166, pp. 1285–1285, 2017.

[6] O. M. Ighodaro and O. A. Akinloye, “First line defence antioxidants-superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPX): Their fundamental role in the entire antioxidant defence grid,” Alexandria Journal of Medicine, Nov. 2017.



PT – 05

Visual, Organoleptic and Physical Detection as Qualitative Indicators of Formalin-containing Food: Experimental Study

on Skipjack Fish Meat (Katsuwonis pelamis)

Johana Z. Wantania1*, and Alfonds A. Maramis1 1Department of Biology, Faculty of Mathematics and Natural Sciences, State University of Manado (UNIMA),

Minahasa, Indonesia

*Corresponding author: [email protected], [email protected]

INTRODUCTION .. To ensure a food contains formaldehyde, the only way is to bring it to a particular laboratory for further analysis, but this method is considered less practical for the general public. This problem encourages researchers to conduct research in order to provide information relating to qualitative indicators that can be used as a marker of formalin-containing food. The purpose of this study was to detect visually, organoleptically and physically the meat of Cakalang fish (Katsuwonis pelamis) which had been intentionally added with formalin. METHODS . This study used a Randomized Block Design (RBD), with 6 categories of concentration of formalin solution used for dipping Cakalang fish (K. pelamis), namely: F1 = 100000 ppm; F2 = 10000 ppm; F3 = 1000 ppm; F4 = 100 ppm; F5 = 10 ppm; and F6 = 0 ppm. Each treatment was repeated 4 times. Parameters observed were visual, organoleptic (numerical), and physical detection. These parameters were observed after 12 hours of treatment, namely by dipping the fish into formalin solution with various concentrations for 5 minutes. Visual indicator data will be analyzed descriptively, while the organoleptic and physical data will be analyzed non-parametric statistics (Kruskal Wallis Test). RESULTS AND DISCUSSION. The results showed that: (1) visually, the fish dipped in formalin solution with high concentrations (100000, 10000, and 1000 ppm) looked fresher and less deformed than those dipped in formalin with low concentrations (100 , 10, and 0 ppm). The fish dipped in formalin was also not infested with flies, in contrast to the fish that was not dipped in formalin; (2) organoleptically, immersion into formalin significantly eliminates foul odor from fish after 12 hours of observation; and (3) physically, dipping into formalin significantly affects the level of hardness of fish meat. CONCLUSION. Visual, organoleptic and physical detection can distinguish fish contains formalin with high or low concentration.



MVS – 01

Reproductive Characters of Senduro Goat at District of Lumajang East Java

Gatot Ciptadi1*, M.N. Ihsan, 2, Agus Budiarto3, M. Mudawamah4 and Arieq Naufal5

1 Faculty of Animal Science, Brawijaya University, . ciptadi @ub.ac.id 2 Faculty of Animal Science, Brawijaya Universityand email 3 Faculty of Animal Science, Brawijaya University and email

Faculty of Animal Science, UNISMA, Malang 5Faculty of Animal Science, Brawijaya University

*Corresponding author: ciptadi @ub.ac.id

INTRODUCTION. Senduro goat is native local animals which raised many in the area of Lumajang District East Java. This goat is considered as a potential new genetic resource for both milk and meat production. Senduro goat has been declared as new breed Indonesia goat at 2014. In general, the character of Senduro goat was identic with Peranakan Etawah (PE), because actually it was selected from PE by the group and Senduro breeding association during a number of years. The aims of this research are characterized reproductive characters of adult female Senduro goat existing in smallholder farm. METHODS . Determination of the location of the research was carried out purposively in 6 villages in Senduro sub-district, Lumajang. The animal material used is 155 adult goats. The method used is a case study. Data is analyzed descriptively. RESULTS AND DISCUSSION. The results showed that the average litter size was 1.83 + 0.69 per parent, while the kidding interval was 281.87 + 37.66 days. Mortality of Senduro goats is low, at 4.93%. The reproductive performance is considered low [1,2]. The incidence of single births was 32.45%, while multiple births reached 67.55, where the two births were the highest reaching 55.62%. REFERENCES [1] Sodiq, A, and MY. Sumaryadi. 2002. Reproductive performans of Kacang and Peranakan Etawag Goat In Indonesia. Journal

Production 4 (3):52-59 [2] G. Ciptadi, Budiarto A., Ihsan M.N., Wisaptiningsih U., Wahyuningsih,2002.Reproductive Performance and Success of Artificial

Insemination in Indonesian Crossbreed Goats in Research versus Small Holder Farm JOURNAL home page:

http://www.aensiweb.com/aejsa.html. American-Eurasian Journal of Sustainable Agriculture 8(7), Special, Pages: 35-38



MVS – 02

Oviduct Specific Glycoprotein Expresion in Goats (Capra aegagrus) Testes and Ovariums

Herlina Pratiwi 1, Aulia Firmawati 1, Herawati2 and Nurul Isnaini3

1,2Veterinary Medicine Faculty, Unniversitas Brawijaya, Puncak Dieng Eksekutif Malang, Indonesia

3Animal Science Faculty, Universitas Brawijaya, Veteran Malang, Indonesia

*Corresponding author: [email protected], phone: +6282257837067

INTRODUCTION Oviduct Specific Glycoprotein (OVGP) is glycoprotein that have both rule in male and female goats reproduction. The OVGP have impportant rule in male reproduction to improve physiological and membrane fluidity and helps increase calcium ions into membranes that stimulate membrane bonding with intracellular cAMP thus enhancing the integrity of intact membranes of spermatozoa. In female reproduction, OVGP have important rule to support fertilitation. In pig and cow, there have been found where was the expression of OVGP in ovarium and it have been used in vivo fertilitation. METHODS. This study was conducted to determine the expression of OVGP in local goat (Capra aegragus) testis and ovarium. The testes and ovariums that used in this study was taken form slaughterhouse whith maximum time three hours from organs harvesting. Observation of OVGP expression in the testis and ovarium was done by immunohistochemical method. RESULT AND DISCUSSIONS. The results showed that OVGP expression in goat testis was seen in the base cells of seminiferous tubules of spermatogonium, spermatocyte cells and in interstitial cells of leydig cells, on the other hand OVGP expression showed in folikel tertier and corpus luteum of ovarium. CONCLUSION. Based on these results it can be concluded that there is an expression of OVGP in local goat testis and ovarium that have importan rules of male and female reproduction. REFERENCE

[1] Coy P., and M. Aviles. 2010. What Controls Polispermi in Mammals, the Oviduct or the Oocyte?. Biological Reviews 85: 593–

605. [2] Laheri, S., M. Deepak, and B. Purvi. 2017. Extra-oviductal Expression of Oviductal Glycoprotein 1 in Mouse:Detection in

Testis, Epididymis and Ovary. India: J. Biosci. 42(1), March 2017, 69–80 * Indian Academy of Sciences. DOI: 10.1007/s12038-016-9657-2. Published online: 11 January 2017. http://www.ias.ac.in/jbiosci.



MVS – 03

REPRODUCTIVE PERFORMANCE dan FERTILITY INDEX SWAMP BUFFALO ( Bubalus bubalis) IN NGAWI

REGENCY

A Budiarto 1, G Ciptadi2, L Hakim 3

1,2,3Lecturer of Faculty of Animal Science, Brawijaya University, Malang


INTRODUCTION. Reproduction is a very important factor in determining livestock productivity. The better reproductive efficiency seen from the low birth spacing is expected to increase the population of buffaloes.The purpose of this research is to know the reproductive performance of productive female swamp buffalo (Bubalus bubalis) in Ngawi Regency, including service per conception (S/C), length of pregnancy, days open (DO), birth interval, conception rate (CR) and fertility index. METHODS . The material used in this research is 162 female buffalos by 40 breeders. The research method used is surveys. The data obtained were analyzed descriptively. RESULTS AND DISCUSSION. The results showed that the buffalo reproduction performance in Ngawi Regency was average service per conception 1.3+0.5 times, length of pregnancy 10.4+0.5 months, days open 3.8+ 0.7months, birth interval 14.3+0.9 months, conception rate 68.5% and fertility index 62.6%. It was concluded that the reproductive performance is still normal in terms of fertility index.so that good reproduction arrangements can increase livestock productivity both in terms of production and reproduction. REFERENCE [1] Chaikhun, T. Hengtrakunsin, R. Rensis, F. D. 2012. Reproductive and Dairy Performances of Thai Swamp Buffaloes under

Intensive Farm Management. Thai J Vet Med. 42(1): 81-85. [2] Fooda, T.A., A.R Elbeltagi, R. Laila, H. Set El-habaeib and S. Awad. 2011. Evaluated of Egyptian Buffaloes crossing with Italian

Buffalloes for Reproductive Traits. J. of American Sci. Vol. 7 (7): 209 - 213



MVS – 04

Effect of Supplementation of Either Powdered or Encapsulated Probiotic on Carcass Percentage, Giblets and

Small Intestinal Morphometric of Local Duck

Sjofjan O1) Adiansah I2) and Sholiha K2) 1)Lecturer of Animal Nutrition and Feed Science Department, Faculty of Animal Science, University of Brawijaya

2)Undergraduate Student of Faculty of Animal Science, University of Brawijaya

Corresponding author: [email protected]

INTRODUCTION . The purpose of this research was to evaluate the effect of supplementation of either powdered or encapsulated probiotic on carcass percentage, giblets and small intestinal morphometric of local duck. METHODS . One hundred twenty male local day old ducks (DOD) were distributed to 6 different dietary groups, included 2 probiotic forms of either powdered (T1) or encapsulated (T2) and 3 levels: 0% (L0), 0.2% (L1), 0.4%(L2). They were reared for 42 days (6 weeks). Variables observed were carcass percentage, giblets (gizzard, heart, liver) and small intestinal morphometric (villus height, villus width, crypt depth). Data were analyzed by Nested of Completely Randomized Design ANOVA and if there was significant effect followed by Duncan’s Multiple Range Test (DMRT). RESULT AND DISCUSSION. The result showed that there was no significant effect (p>0.05) of the form of either powdered or encapsulated probiotic on carcass percentage, giblets weight, and small intestinal morphometric. However, increasing level of probiotic have a significant effect (p<0.05) on carcass percentage, villus height, and villus width, but did not significantly affect giblets weight and crypt depth. It could be concluded that supplementation of either powdered or encapsulated probiotic have similar result. It is suggested to use 0.4% of encapsulated probiotic in local duck diet.



MVS – 05

IN VITRO FERTILIZATION OF CRYOPRESERVED GOAT OOCYTE S IN DIFFERENT CRYOPROTECTANTS

Sri Wahjuningsih 1), Muhammad Nur Ihsan 1),Gatot Ciptadi 1), Helly Nurul Karima 2)

1)Department of Animal Production,Faculty of Animal Husbandry, University of Brawijaya, Indonesia

2) Central Laboratory of Life Sciences, University of Brawijaya, Indonesia

Corresponding author : [email protected]

INTRODUCTION . The aim of this research was to examine the rate of fertilization of in vitro matured goat oocytes using three different cryoprotectans. METHODS . Iso-osmolar concentrations of 1.5 M of ethylene glycol (EG), glycerol (GLY) and dimethylsulfoxide (DMSO) were contrasted for their effectiveness as cryoprotectans. Ovaries were obtained from local abattoir and were brougt to the laboratory in physiological saline 0.9% (w/v) at 25 to 30oC within 3 h. Follicular oocyte were collected by aspiration follicle with 2-6 mm diameter.This study exclusively selects oocytes with compacted ooplasm and cumulus cells. Following a 26 h incubation, oocytes were cryopreserved and then fertilized. Data were pooled from 10 replicates. RESULT AND DISCUSSION. Statistical significance of differences among groups were analysed using Chi-square analysis. Percentage of fertilized oocytes after freeze thawing in EG (48.38%) was higher (P<0.05) than in GLY (45.05%) or DMSO (44.50 %). The cleaved rate of cryopreserved oocytes in EG (25.28%) was higher (P<0.05) than of those frozen in GLY (19.28%) and DMSO (17.21%). This experiment suggests that EG was the most suitable cryoprotectant for cryopreservation of post-thaw in vitro matured goat oocyte. REFERENCES [1] Gabriel PR, Tornes P, Fratto MC,Cisale H, Claver JA,Lombardo DM and Fischman ML. 2017 Effects of different sucrose

concentrations on vitrified porcine pre antral follicles : qualitative and quantitative analysis. Cryobiology 76: 1-5 [2] Hochi S, Kimura K, Ito K,Hirabayashi M . 1997 Effect of nuclear stages during in vitro maturation on the survival of bovine

oocytes following vitrification Theriogenology 47 : 345 [3] Men H, Monson RL,Rutledge JJ. 2002 Effect of meiotic stages and maturation protocols on bovine oocyte’s resistance to

cryopreservation Theriogenology 57:1095-1103 [4] Wiesak T,Wasielak M, Zlotkowska A and Mileski R. 2017 Effect of vitrification on the zona pelucida hardening and follistatin

and cathepsin, B genes expression and developmental competence of in vitro matured bovine oocyte. Cryobiology 76 : 18-23 [5] Wahjuningsih S and Isnaini N. 2014. Supplementation of goat follicular fluid in TCM 199 based medium on in vitro matured

oocyte. American Eurasian J of Suistanable Agriculture 8(7) : 39-40



MVS – 06

The Activity of Casein Derived from Goat Milk Yogurt as an Antioxidant on Histophatology of Rat’s Liver Exposured

by 2,3,7,8-Tetrachlorodibenzo-p-Dioxin (TCDD)

Chanif Mahdi 1, Ajeng Erika Prihastuti Haskito 2, Masdiana C. Padaga3, Anna Roosdiana1* 1 Department of Chemistry, Faculty of Mathematics and Science, Brawijaya University, Malang 65145, Indonesia

2 Veterinary Pubic Health Laboratory, Faculty of Veterinary Medicine, Brawijaya University, Malang 65145, Indonesia


INTRODUCTION. 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) is a toxic contaminant which persistent in the environment. Accumulation of this compound on the body through food chain can generate adverse effect in human health, such as oxidative stress in various organs. The aim of this research was to observe the activity of casein derivad from goat milk, which is well known to have potential as an antioxidant, to prevent oxidative stress due to TCDD intoxication. METHODS. A total of twenty four male Wistar rats of twelve weeks old were used in this research. The rats were divided into six groups: (1) control group, (2) placebo group which given casein 600 mg/kgBW, (3) TCDD group given with TCDD 100 ng/kgBW, (4) group P1 given casein 300 mg/kgBW and TCDD, (5) group P2 given casein 600 mg/kgBW and TCDD, (6) group P3 given casein 900 mg/kgBW and TCDD. Provision of casein and TCDD were given orally for 21 days. Liver sample collected for histophatological examination with hematoxylin Eosin (HE) stained. Experimental method was based on completely randomized design. The histopathological were analyzed by Kruskall-Wallis test followed by Mann-Whitney test to know the differences of the treatments. RESULTS AND DISCUSSION. showed that casein could protect the rats liver from damage in TCDD intoxicated rats. Significant increase (P<0.05) shows from group given with casein dose 900 mg/kgBW. REFERENCE [1] Hassoun, E. A., F. Li, A. Abuchaban and S. J. Stohs. 2000. The Relative Abilities of TCDD and Its Congener to Induce Oxidative

Stress in the Hepatic and Brain Tissue of Rats after Subchronic Exposure. Toxicol. J. 145: 103-113. [2] Hutahaean, S. 2010. Kajian Palatogenesis Mencit (Mus musculus L.) Akibat Pemberian 2,3,7,8-tetrachlorodibenzo-p-dioxin.

Disertasi Sekolah Pasca Sarjana Universitas Gadjah Mada. Yogyakarta. 1-3. [3] Karami, M., M. G. Khansari, M. Rezayat, B. Minaei, M. Abdollahi, O. Sabzevari. 2001. Histopathological Study of TCDD by

Isolated Rat Liver Perfusion System. Medic. J. of the Islamic Republic of Iran. Vol. 15 (1) : 55-60. [4] Kumalaningsih, S. 2006. Antioksidan Alami : Penangkap Radikal Bebas. Trubus Agrisarana. Surabaya. 7-86.



MVS – 07

The Conception Rate Of Artificial Insemination By Using Sexing Frozen Semen On Ongole Crossbred Cows

Trinil Susilawati 1*, Aulia Puspita Anugra Yekti2, Kuswati3, Wike Andre Suwito4 and Asri

Nurul Huda 5

1 Veteran Street Malang, Indonesia Animal Husbandry Faculty, University of Brawijaya, Indonesia, 2 Veteran Street Malang, Indonesia Animal Husbandry Faculty, University of Brawijaya, Indonesia, 2 Veteran Street

Malang, Indonesia Animal Husbandry Faculty, University of Brawijaya, Indonesia, [email protected] 2 Veteran Street Malang, Indonesia Animal Husbandry Faculty, University of Brawijaya, Indonesia


INTRODUCTION The artificial insemination by using sexing semen is aimed for getting offspring with sex as expected, this research was conducted by using percoll density gradient centrifugation method which has successfully frozen with percentage of motility is > 40% and proportion of spermatozoa Y is 89% (The identification was based on spermatozoa head size) produced by AI centre Singosari Malang. The purpose of this research is to evaluate the conception rate of Ongole crossbred cows which were inseminated with sexing frozen semen. The material used was 69 head of crossbred cows inseminated with non-sexing frozen semen and 69 head of crossbred cows inseminated with sexing frozen semen. the Age was around 2-7 years old, have given birth normally, the cows were housed in traditional farm in District of Palang, Tuban district of East Java province. METHODS The research methods were Ongole CrossBred cows that have shown signs of estrus such as vulva swelling, red, warm and transparent mucous and make a noise sound were inseminated after 6-8 hours later with non-frozen semen or sexing frozen semen in deep insemination. The frozen semen was previously thawed by using tap water (20-22 C for 20 seconds). The cows were injected by using vitamin Bio ATP and given the additional feed with 10% protein 3 kg/day for 3 days after inseminated. The sign of estrus was observed on 19-22 days after inseminated to confirm the Non Return Rate (NRR-1). The cows that showed the sign of estrus were considered as non-pregnant cows, meanwhile the cows that did not show the sign of estrus were considered as pregnant cows. Furthemore the conception rate was observed by using rectal palpation to confirm the pregnancy of cows, then the Ultra Sono Grafi (USG) was used to confirm the cows which have the reproductive problems such as did not showed the sign of estrus, however the cows were not pregnant based on rectal palpation. RESULTS AND DISCUSSION. The results showed that NRR-1 and NRR-2 non-sexing frozen were 89.8% and 97.2%, while in sexing frozen semen was 81.2 and 89.9%. The value of Conception Rate in the first AI (CR-1) and in the second AI (CR-2) on non-sexing semen were 46,2% and 43,8% while in sexing frozen semen were 53, 8% and 61,5%. Based on these data showed that there was a decreasing of NRR value to CR value, the differences value of CR-1 and CR-2 on non-sexing frozen semen were 49.2% and 53% while in sexing frozen semen were 27.3% and 28.3%. furthemore the USG results showed that the differences of NRR and CR value mainly caused by either a repeat breeder or an AI failure or an ovarian hypofunction. This is due to the recipient used were the offspring of crossing between filial Ongole with Limousin or Simental which cannot bear to be maintained in the tropics condition with hot temperature, not resistant to ectoparasites and endoparasites and the poor feed quality and quantity. REFERENCE [1] Boro, P., B. C. Naha., A. Madkar and C. Prakash. 2016. Sexing of Semen in Bulls: A Mini Review. International Journal of

Applied Research. 2 (4): 460-462. [2] Cooke, R. F., D. W. Bohnert., B. I. Cappellozza., R. S. Marques., T. Delcurto and C. J. Mueller. 2014. Incorporation of Sexed

Semen Into Reproductive Management of Cow-Calf Operations. Livestock Science. 163: 165-171. [3] JainudeenM.R and E.S.E Hafez 2008a Pregnancy Diagnosis. In Farm Animal Reproduction . ed by E.S.E. Hafez and B. Hafez 7th

Edition . Blackwell Edition : 395 – 404 [4] Kusumawati, E.D, N Isnaini, A.P.A Yekti, M. Lutfi, L.Affandhy, D.Pamungkas, Kuswati, A.Ridhowi, H.Sudarwati, T. Susilawati

and S. Rahayu .2017. The Quality Of Sexed Semen On Filial Ongole Bull Using Percoll Density Gradient Centrifugation Method Asian Jr.of Microbiol.Biotech.Env.Sc. 19 (1) : 189-100

[5] Susilawati,T, S. Rahayu, S.Udrayana, H. Sudarwati and E. Nugroho (2014) Effect of Different Centrifugation Duration on Simmental Bull Sperm Quality and Membrane Status after Sexing, Cooling and Freezing Process. American-Eurasian Journal of Sustainable Agriculture , 8 (7) : 28-34



MVS – 08

Individual Mutations in Indonesian Local Ettawah Goats Based on The GDF9 Gene

Mudawamah Mudawamah1, I.D. Ratnaningtyas1, M.Z Fadli 2 and G.Ciptadi3

1Animal Science Department, University of Islam Malang, MT Haryono 193 Malang, Indonesia 2Medicine Department, University of Islam Malang, MT Haryono 193 Malang, Indonesia

1Animal Science Department, University of Brawijaya, Veteran Malang, Indonesia

*Corresponding author: : [email protected]

INTRODUCTION .. A prolific trait was very important to determine the litter size in goats including Indonesian Local Ettawah Goats (ILEG), and one of the genes that influence the prolific trait was the GDF9 gene. The purpose of this study was to describe the amino acid expression of the GDF9 gene sequencing in ILEG compared with Genbank accession number GU784823.2. Capra hircus GDF9. METHODS This research method was an experiment which includes the sampling of 21 does who had given birth more than once with low, medium and high prolific categories. Furthermore, samples were PCR with exon 1 GDF9 gene and sequencing results were carried out by amino acid alignment with BioEdit software. RESULTS AND DISCUSSION. The results showed that there were 3 variants in the exon 1 GDF9 gene in ILEG which was located at the location of 27 amino acid residues (9.25% of the total sample with medium-high birth type), 61 (100% of the total sample with low-high prolific type) and 85 (14.29% of the total sample with a prolific type of medium-high). At the 27th residue there was a change in the proline amino acid to alanine (CCT to GCT), at the 61st residue there was no change in the amino acid Leucine but there was a change in the base N compound (CTA to CTC) and at 85 residues there was a change in acid residue amino Alanine becomes Glycine (GCT to GGT). REFERENCE [1] R. Xue-qin, L. Jian-bin, D. Zhi-yong, Q. Cheng, and W. Jia-fu. Zoology Research. 30, 593-602 (2009). [2] R. Dutta, B Das, S. Laskar, D.J. Kalita, P. Borah, G. Zaman

and D. P.Saikia. African Journal of Biotechnology. Vol. 12, 6894-

6900 (2013). [3] Mudawamah, I.D. Retnaningtyas, V.M.A.Nurgiartiningsih, C.D.K Bottema. Proceeding The 16th Asian-Australian Association

Of Animal Production Societies, 16, 124-127 (2014). [4] K.Q. Tang, W.C. Yang, S.J. Li and L.G. Yang. Genet. Mol. Res. 12: 390-399 (2013)



MVS – 09

Malondialdehyde (MDA) Level and Protein Profile of Serum After Calving Towards The Provision of Selenium-Vitamin

ETM on Dairy Cow Frisien Holstein (FH)

Sri Rahayu1*, Widya Ayu Prasdini2 , Mochammad Sasmito Djati1, Sasangka Prasetyawan3, Gatot Ciptadi 4

1Biology Department, Faculty of Natural Sciences, Brawijaya University, Malang, Indonesia 2Training Center for Animal Husbandry, Batu, East Java, Indonesia

3Chemistry Department, Faculty of Natural Sciences, Brawijaya University, Malang, Indonesia 4Faculty of Husbandry, Brawijaya University, Malang, Indonesia


INTRODUCTION Free radicals play an important role in reproductive biology because free radicals are molecules that play a role in reproductive function. However, free radicals can cause damage to the cell membrane, which can result in decreased fertility. This study aims to determine effectiveness of selenium and vitamin ETM intramuscularly to the serum MDA level and protein profile after calving. METHODS . Dose and timing of the Selenium and Vitamin ETM based on research conducted by Widya et al (2014). Twenty pregnant FH cows were use in this experiment, devided into four groups, the control group (K0) (without injection of selenium and vitamin E), Group 1 (K1) injections of 0.5 mg / ml selenium + 50 mg / vitamin ETM ml, Group 2 (K2) were injected 1.5 mg / ml selenium + 50 mg / ml of vitamin ETM and Group 3 (K3) were injected 2 mg / ml selenium + 100 mg / ml vitamin ETM. Selenium-vitamin ETM given intramuscularly at the age of 7 and 8 months of pregnancy, two weeks before birth, 7 days and 14 days after calving. RESULTS AND DISCUSSION. The results showed that, MDA levels decreased with increasing doses of Selenium and vitamin ETM on the 45th day after calving. There was no difference in protein profile of serum in all of treatment groups. REFERENCES [1] Castillo C, Hernandez J, Bravo A, Lopez-Alonso M, Pereira V, Benedito J.L.. 2005. Oxidative status during late pregnancy and

early lactation in dairy cows. The Veterinary Journal. 169 : 286–292 [2] Da Silva, F. M., A. Marques. & A. Chaveiro. 2000. Reactive Oxygen Species : A Double – Edged Sword in Reproduction. The

Open Veterinary Science Journal. 4:127-133. [3] Higuchi, H., Erino I., Hidetoma I., Shin O. & Hajime N. 2013 Effects of Vitamin E Supplementation on Celluar α Tocopherol

Concentration of Neutrophils in Holsteins Calves. The Canadian Journal of Veterinary Research. 77:120-125. [4] Jishage, Kou Ichi., Takanori T., Tsuneo I., Norihito S., Shigeo S., Toshio M., Toshio H., Hiroyuki A. & Hiroshi S. 2005. Vitamin

E is Essential for Mouse Placentation But Not for Embryonic Development Itself. Biology of Reproduction. 73:983-987.



MVS – 10

The Profile Of Red Blood Cells In Sumba Ongole Cattle Is Maintained In Dryland Areas

Nemay A Ndaong1, Annytha Detha2, Nancy DFK Foeh3, Dikhi O Marumata4, Roni R

Kapida4, Faustin M Kaka4, Frans U Datta1 1Divion of pharmacology and biochemistry, Veterinary Faculty, Nusa Cendana University 2Department of Animal

Diseases and Veterinary Public Health, Veterinary Faculty, Nusa Cendana University, Kupang, Indonesia 3Divion of Animal Reproduction, Veterinary Faculty, Nusa Cendana University 4Veterinary Faculty, Nusa Cendana

University, Kupang, Indonesia.


INTRODUCTION .. This study aims to determine the picture of red blood cells in Sumba Ongole Cattle that are kept on dry land area. The research sample is 12 adult Sumba Ongole Cattle. METHODS Tool used disposible syringes 3 ml (one med®), venoject tube, needle size 18G, ethylediaminaminatetraasetat (EDTA) tube, glass object, glass cover, diluent pipette. Blood sampling is preceded by finding a jugular vein located in the neck region. After finding the jugular vein the part to be stabbed was given cotton alcohol. Blood is taken as ± 3 ml with venoject and no needle. 18G connected to EDTA tube. Examination of blood samples using MEK-8222J / K CELLTA-F tool. The results of examination on total erythrocyte of sumba ongole (SO) obtained mean total erythrocyte ie 9,43-10,33 x 106 / µl. The range of normal values of cow erythrocytes is 5.51-8.9 x 106 / µl (Egbu et al., 2013). RESULTS AND DISCUSSION. The results of the examination on hemoglobin content of sumba ongole (SO) obtained mean hemoglobin level of 13.97-15.70 g / dL. The range of normal values of cow hemoglobin level is 8,5-13,6 g / dL. The result of hematocrit score of sumba ongole (SO) is obtained by average hematocrit value that is 44,67-50,50%. The range of normal hematocrit values in cows is 30-50%. Check up result. The normal range of MCV values in cows 47-56 fl. The results of the examination on the value of MCH cow sumba ongole (SO) obtained an average MCH value of 14.43-15.87 pg. The range of normal MCH values in cows is 12-18 pg. REFERENCE [1] Dvya, A. B., Mandal, A., Biswas, A. S., Yadav., A. K. Biswas. 2014. Effect of Dietary Moringa oleifera Leaves Powder on

Growth Perfomance, Blood Chemistry, Meat Quality and Gut Microflora Of Broiler Chicks. Animal Nutrition and Feed Technology. 14: 349-357.

[2] Esfandiari, A., Widhyari, S. D., Sajuthi, D., Maylina, L., Mihardi, A. P., Supriyatna, E. R., et al. (2016). Panduan Pemeriksaan Laboratorium Patologi Klinik. Bogor: IPB press.

[3] Harvey, John W. 2012. Veterinary Hematology: A Diagnostic Guide and color Atlas. Missouri (US): Elsevier.



P – 01

Pathophysiological Changes in Salt Loaded Female Spontaneously Diabetic Torii (SDT) Fatty Rats

Soon Hui Teoh1, Katsuhiro Miyajima 1, Masami Shinohara2, François Briand3, Dai

Nakae1, and Takeshi Ohta4* 1 Tokyo University of Agriculture, Japan, [email protected]

2 CLEA Japan, Inc., Japan, [email protected] 3 Physiogenex, France, [email protected]

4 Japan Tobacco Inc., Japan, [email protected]


INTRODUCTION. . Type 2 diabetes mellitus had become an international health concern with its growing number of patients globally. At the same time, excessive salt consumption was also seen as a major cause of diseases such as hypertension and may expedite renal complication in diabetic patients. In this study, we investigated the effects of excessive sodium chloride supplementation on the kidney and the liver of the female Spontaneously Diabetic Torii (SDT) fatty rats, an obese type 2 diabetic model. METHODS Female SDT fatty rats and normal Sprague Dawley (SD) rats at 5 weeks of age were loaded with 0.3% NaCl in drinking water for 12 weeks. Blood serum and urinary parameters were observed throughout the experiment and kidney and liver samples were examined in histopathological analyses. RESULTS AND DISCUSSION. Significant changes in the body weight and blood glucose, ALT, and AST levels were observed in the salt loaded SDT fatty rats. In the kidney, slight Armani Ebstein changes, tubular degeneration, hyaline cast, inflammatory cell infiltration and fibrosis were observed in female SDT fatty rats while the levels of some changes were higher in the salt loaded group. In the liver, histological changes such as activation of Ito-cells and inflammatory cell infiltration were significantly observed in the salt loaded group while the degree of hepatic fibrosis in the salt loaded group was comparable to that in the unloaded group. Acceleration of histopathological changes in salt loaded SDT fatty rats shows that excessive salt consumption may act as a diabetic pathology exacerbation factor.

REFERENCE [1] Katsuda et al., Anim. Sci. J. 85, 588 (2014). [2] Katsuda et al., J. Diabetes Res. 363126 (2014). [3] Ishii et al., World J. Gastroenterol. 21, 9067 (2015).



P – 02

Interaction of Acetaminophen and Caffeine Towards Cyclooxygenase-2 (COX-2) in Inhibition of Prostaglandin

(PGH) Synthesis

Gabriella C. Krisnamurti1,2

, Novia C.2, Christyanita P. Ekasari

2, Yuli K. Br Tarigan

2, Azula

V. Daniwati2, Fatchiyah Fatchiyah

1,2,*

1Department of Biology, Faculty of Mathematics and Natural Science, University of Brawijaya

2Research Centre of Smart Molecule of Natural Genetics Resources, Brawijaya University, Indonesia


INTRODUCTION. Pain is a response of inflammation and it can be relieved by using analgesic drugs such as acetaminophen. Acetaminophen worked by inhibit COX-2 to synthesize prostaglandin and caffeine can optimize the reaction. Objectives of this study are identifying the binding site of COX-2 with acetaminophen and caffeine and also determining the binding energy of COX-2 with acetaminophen and caffeine. METHODS . The COX-2 structure used for this study is PDB ID 1CVU. Ligands used for this study is PubChem ID 1983 (acetaminophen) and 2519 (Caffeine). Preparation of COX-2 were using Discovery strudio v.16 and ligands were using PyRx. Docking was done by PatchDock after preparation by interacting COX-2 with acetaminophen then adding caffeine so does COX-2 with caffeine the adding acetaminophen. Data analyzed using Discovery Studio v.16. RESULTS AND DISCUSSION. Caffeine bind to COX-2 with less energy, compared with acetaminophen (Table 1). Less energy means that molecules can bind easily. Energy needed to bind are quite similar, thus acetaminophen bind with COX-2 with less energy, with or without caffeine. Amino acid residues binding with ligand are the active site of COX-2. COX-2 and acetaminophen have interactions at Ser 530, Gly 526, Ala 527, Val 349, and Leu 352 (Table 2). COX-2 binding sites with acetaminophen and caffeine depend on compound reacted first. Table 3 and figure 3 show the differences of ligand’s binding sites and position. COX-2 active sites are in hydrophobic channel located between Arg 120, Glu 524, and Tyr 355. Ligands bind in hydrophobic channel and inhibition sites for COX-2 inhibition. Interaction occur in Ser 530, Val 523, and Tyr 385 which are COX-2 inhibition sites. Both caffeine and acetaminophen can bind with COX-2 active sites, thus acetaminophen only can react with COX-2. Caffeine can optimize acetaminophen reaction, but side effect still unknown.

Table 1. Energy binding of COX-2 with acetaminophen and caffeine

Table 2. Interaction of COX-2 with acetaminophen



a

b

c

Figure 1. Interaction of COX-2 with ligands: a) COX-2 with acetaminophen, b) COX-2 and acetaminophen

and caffeine, c) COX-2 and caffeine with acetaminophen Table 3. Interaction of COX-2 with acetaminophen and caffeine COX-2

+ A + C

COX-

2 + C + A

REFERENCE [1] Sellers, R.S., A. Radi & N.K. Khan. Veterinary Pathology 47(4): 601-613 (2010) [2] Turtle, E.J., J.W. Dear & D.J. Webb. Br J Clin Pharmacol 75(6): 1396-1405 (2013) [3] Ricciotti, E & G.A. FitzGerald. Arterioscler Thromb Vasc Biol 31(5): 986-1000 (2011) [4] Bebenista, M. J, & J.Z. Nowak. Acta Poloniae Pharmaceutica - Drug Research, 71(1) : 11-23 (2014) [5] Derry, C.J., Derry S & Moore R.A. Wiley (2012) [6] Fiebich, B.L., K. Lieb, M. Hull, B. Aicher, J. van Ryn, M. Pairet & G. Engelhardt. Neuropharmacology 39: 2205-2213 (2000) [7] Jovel, Espinosa & S. Mejia. Neurologia. 32(6): 394-398 (2017) [8] Zhang C, G. Vasmatzis, J.L. Cornette & C. DeLisi. J Mol Biol. 267(3):707-26 (1997)



P – 03

Effect of Combination Core Stability Exercise and Contract Relax Exercise On Hamstring Flexibility

Aisyah L. Na’ima1*, Gadis M. Sari2, Dwikora N. Utomo3 1Department of Sport Health Science, Airlangga University, Surabaya 2Department of Sport Health Science, Airlangga University, Surabaya

3Department of Orthopedics and Traumatology, Dr. Soetomo Hospital, Surabaya


INTRODUCTION. Hamstring is a muscle group that often has a reduced flexibility compared to other muscle groups. This hamstring flexibility reduction can lead as a low back pain risk factor. Nowadays, the effect of core stability and contract relax exercise on hamstring flexibility is still unclear, thats why the aim of this research is to know about this exercise on hamstring flexibility. METHODS. This research is experimental with 24 subjects of healthy female students who participated in the training program for 4 weeks consisting of 3 times per week. Subjects were randomly assigned to one of three groups: (1) core stability-CS (n=8), (2) contract relax-CR (n=8), (3) core stability combined contract relax-CS+CR (n=8). The hamstring flexibility was assessed with sit and reach test using the sit and reach box. The examinations were conducted by observers twice, prior the training program and 2 days after the last session of exercise. Two subjects who did not participate in all three exercise sessions were excluded from the analysis. RESULTS AND DISCUSSION. 22 subjects were analyzed (CS,n=7; CR,n=8; CS+CR,n=7). The paired t test analysis of each group showed significant CS (p=0.03), CR (p=0.00), CS+CR (p=0.01) in increasing of result sit and reach test was observed. There was a difference between the three groups by the ANOVA test (p=0.00). This can occur through reciprocal inhibition mechanism that occur in the 2 muscle targets of the muscles of the hip extensor and lumbal extensor thus allowing for increased hamstring flexibility. REFERENCE [1] Reis, F. J Jandre, Adriana, R Macedo. 2015. Influence of Hamstring Tightness In Pelvic, Lumbar and Trunk Range Of Motion In

Low Back Pain And Asymptomatic Volunteers During Forward Bending. Asian Spine Journal. Vol. 9, No.4, Hh. 535-540. [2] Kuszewski MT, Gnat R, dan Gogola A. 2018. The Impact Of Core Muscle Training On The Range of Anterior Pelvic Tilt In

Subject With Increased Stiffness Of The Hamstring. Human Movement Science of Journal, Vol. 57, Hh. 32-39. [3] Magalhaes, Francisco Elezier, Arlindo Rodrigues, Harnold’s Tyson, Rayele Pricila, Ezaine Costa, et al., 2015. Comparison of

The Effects of Hamstring Stretching Using Proprioceptive Neuromuscular Facilitation With Prior Application of Cryotherapy or Ultrasound Therapy. Journal Physical Therapy Science. Vol. 27, No. 5, Hh. 1549-1553.

[4] Czaprowski, Dariusz, Justyna Leszczewska, Aleksandra Kolwicz, Paulina Pawlowska, Agnieszka Kedra, Piotr Janusz, Tomasz Kotwicki. 2013. The Comparison of The Effects of Three Physiotherapy Techniques on Hamstring Flexibility un Children: A Prospective, Randomized, Single-Blind Study.

[5] Hasebe, Kiyotaka, Yu Okubo, Koji Koneoka, Kohei T, Daisuke S, Koichi S. 2016. The Effect Of Dynamic Stretching On Hamstrings Flexibility With Respect To The Spino-Pelvic Ryhtm. The Journal Of Medical Investigation. Vol. 63, Hh. 86-90



P-04

Direct Effect Short Wave Diathermy Therapy and Exercise with Quadriceps Bench Against Knee Osteoarthritis Patients

with Functional Capabilities

Dimas Arya Nugraha1*, Ratna D Haryadi2 , and Siswoyo Cahyono3 1 Departement of Physical Therapy, Airlangga University, Surabaya, [email protected]

2 Departement of Physical Medicine and Rehabilitation Faculty of Airlangga University, Dr, Soetomo Hospital, Surabaya, [email protected]

3 Departement of Physical Therapy, Dr. Soetomo Hospital, Surabaya, [email protected] *Corresponding author: [email protected]

INTRODUCTION .. Osteoarthritis (OA) is a disorder of joint cartilage characterized by changes in the clinical, histological and radiological. OA knee associated with various pathological deficits such as pain, decreased range of motion of the knee joint and the quadriceps muscle nirguna atrophy. OA pain in the knee are strongly linked with a reduction in quadriceps muscle strength which is the main stabilizer of the knee joint, causing instability of the knee joint and the resulting decrease in functional ability. Physiotherapy can reduce pain and improve functional ability immediately after treatment. METHODS . This study is observational analytic and design research using cross-sectional design. Subjects in this study were 30 patients with osteoarthritis of the knee in outpatient IRM Hospital Dr. Soetomo aged 40-70 years were taken with consecutive sampling technique the period from march to april 2015, an assessment of functional ability before and after treatment using a scale jette. RESULTS AND DISCUSSION. The data in normal distribution and statistical test is then performed using paired t-test. Jette scale mean pre- and post-treatment with Short Wave Diathermy (SWD) modalities and exercises with Quadriceps Bench (QB) in patients with knee osteoartriitis show is different, with a value of less significance α (p <0.05). This can occured because, blood flow in the tissues increase, throw away the metabolite residual, and decrease muscle spasms, so the pain is reduced and the functional ability of the knee increases. REFERENCES [1] Corwin, Elizabeth J. Buku Saku Patofisiologi. Jakarta : Penerbit Buku Kedokteran EGC (2007). [2] Darrow M. The Knee sourcebook. McGraw-Hill, Chicago (2002). [3] Depkes. RI. Standar Pelayanan Fisioterapi di Sarana Kesehatan. Jakarta (2008). [4] Heru P Kuntono. Penatalaksanaan Fisioterapi pada Kondisi Osteo Arthritis. Temu Ilmiah IFI, Kediri (2005). [5] Hoogland, R. Enraf Nonius Equipment Protocols, B.V. Enraf Nonius, Delf, Netherlands (2010). [6] Kisner C, Colby L.A. Therapeutic Exercise :Foundations and Techniques. 5th ed. Philadelphia, Pa: FA Davis Co (2007). [7] William E. Prentice. Rehabilitation techniques for sports medicine and athletic training. 5th ed (2004).



P-05

Pathophysiological Evaluation of The Liver in Western Diets Fed Insulin Resistant Zucker Fatty Rats

Mariko Maekawa1, Tomohiko Sasase1*, Tomoyuki Saito1, Yasufumi Toriniwa1,

Yukihito Ishii 1, Atsuhiro Uemura1, Katsuhiro Miyajima 1, 2, Kinuko Uno2, Yuki Shirai 2, Dai Nakae2, and Takeshi Ohta1

1 Japan Tobacco Inc., Japan, [email protected] 2 Tokyo University of Agriculture, Japan, [email protected]

*Corresponding author: [email protected]. In these decades, the Westernization of

food is closely related to the development of metabolic diseases such as nonalcoholic steatohepatitis (NASH). In the present study, we investigated pathophysiological changes in the livers of obese Zucker fatty

(ZF) rats induced by feeding Western diets. METHODS . Male ZF rats were fed sucrose/fat/cholesterol-enriched diets (Western diets; WD) or standard diets (SD) for 18 weeks, from 7 to 25 weeks of age. Body weight, food intake, and biochemical parameters were periodically measured, histopathological analyses

were performed at 25 weeks, and mRNA expression in the liver was also determined. RESULTS. Zf rats fed wd (zf-wd rats) developed obesity, hyperinsulinemia, hyperglycemia, and hyperlipidemia. Blood levels of

alanine aminotransferase (alt) and aspartate aminotransferase (ast), markers of liver damage, were increased compared with those in zf rats fed sd (zf-sd rats). Hepatic lesions including fibrosis and necrosis were

observed in the zf-wd rats at 25 weeks; however, these pathological changes in the liver were not found in the zf-sd rats. Oxidative stress markers also increased in the livers of zf-wd rats. Hepatic mrna expression related to inflammation and fibrosis increased in the zf-wd rats; however, mrna expression related to lipid synthesis and microsomal triglyceride transfer protein (mtp) in the zf-wd rats were decreased. Zucker lean rats fed wd showed similar but slight changes of the liver compared to zf-wd rats. Hepatic lesions, such as inflammation, fibrosis, and necrosis, were observed in the zf rats fed wd. Sucrose/fat/cholesterol-enriched

diets induced significant lipotoxicity in the livers of animals in this insulin-resistant model.

REFERENCES [1] Saito et al., J. Toxicol. Pathol. (2018)



P - 06

The Immunogenicity Of Polyclonal Antibody From Induced Vibrio Parahaemolyticus Membrane By Elisa Method

Mery Pattipeilohy1, Sundari2*

1Biology Education, Faculty of Teacher Training and Education, Patimura University, Ambon, Indonesia 2Biology Education, Faculty of Teacher Training and Education, Khairun University, Ternate, Indonesia


INTRODUCTION Polyclonal antibodies are antibody proteins formed from interaction between antigens that enter a body. It results in immunogenicity. The process of generating polyclonal antibodies begins with purifying a membrane protein. A membrane protein that is potential to make an antibody is the outer membrane protein (OMP) of bacterium V. parahaemolyticus. METHODS. In the current work, this can be carried out by measuring mitochondrial functions of the live cells in real time. This protein is injected into an animal’s body as the target antigen so that an antibody can be produced. The antibody is obtained by taking the animal’s blood sample. It is then isolated in a serum until it generates a polyclonal antibody. ELISA (Enzyme-Linked Immunosorbent Assay) is a technique normally used to measure the concentration of an antibody or an antigen in blood or to detect their existence in a sample. The aim of this research was to analyse the immunogenicity of polyclonal antibodies produced from Vibrio Parahaemolyticus membranes that were induced using ELISA method. RESULTS AND DISCUSSION. Research findings indicate that the highest OD value of ELISA (0, 404) was obtained on the third week of 1/100 dilution. The highest value of antigens and antibodies interaction was found on 1/100 dilution. Immunization before booster generated relatively small antibody titres because the rat’s body was not ready to provide an immune response.

REFERENCES

[1] Abdallah F., R., Lagha., Ellafi A., Abdelkader N., Rousselle J.C. (2012). Identification Of Outer Membrane Proteins Altered in Response to UVC-Radiation in Vibrio parahaemolyticus and Vibrio alginolyticus. Indian Journal Microbiol (Oct–Dec 2012) 52(4):660–665.

[2] Lequen. (2005). Enzyme Immunoassay (EIA)/Enzyme-Linked Immunosorbent Assay (ELISA)". Clinical Chemistry 51 (12): 2415–2418.

[3] Ramandi, M.F., Moghaddam, A. N., Arabsalmany ,F., Asgari, S., Renani, S. A., Ahmadi, K., (2014). Production And Characterization Of Monoclonal And Polyclonal Antibody Against Recombinant Outer Membrane Protein. American Journal of Immunology 10 (1): 56-62, 2014.



P – 07

Isolation Of Lactic Acid Bacteria From Ruments Of Cattle As A Starter In The Manufacture Of Silage

Nancy DFK Foeh1* , Nemay A Ndaong2, Rambu EM Mala3, Elisabet Beribe3, Putri L

Pau3, Annytha Detha4, Frans U Datta2 1Divion of Animal Reproduction, Veterinary Faculty, Nusa Cendana University

2Divion of pharmacology and biochemistry, Veterinary Faculty, Nusa Cendana University 3Veterinary Faculty, Nusa Cendana University, Kupang, Indonesia.

4Department of Animal Diseases and Veterinary Public Health, Veterinary Faculty, Nusa Cendana University, Kupang, Indonesia


INTRODUCTION .. The purpose of this research is to isolate lactic acid bacteria found in rumen liquid of bali cattle at slaughterhouse of Oeba City of Kupang, and to determine the benefits of lactic acid bacteria on fermentation process of rice straw silage as animal feed based on the quality of rice straw given starter of lactic acid bacteria which is isolated from rumen fluid. METHODS . This research will be conducted from September to November 2017. The research was conducted at Veterinary UPT Laboratory of Animal Husbandry Service of NTT Province and at Animal Disease Laboratory and Kesmavet Faculty of Veterinary Medicine, Nusa Cendana University. The research materials used include rumen fluid, MRS media (De ManRogosa-Sharpe) agar, MRS (De Man-Rogasa-Sharpe) broth medium, PBS (Phospat Buffered Saline) solution, SIM media (Sulfide Indole motility), effective microorganism (EM4 TM). Intake of rumen cow fluid taken from slaughterhouse Oeba, Kupang City. Isolation of lactic acid bacteria from rumen fluid was done by growing lactic acid bacteria on MRS medium (De Man-Rogosa-Sharpe) agar. One way of identifying bacteria is by doing Gram staining, identification of lactic acid bacteria includes catalase test, motility test on SIM media (Indole motility Sulfide). Lactic acid bacteria that have been cultured on MRS Broth media. After preparation of lactic acid bacteria inoculum, followed by testing total plate count to calculate total lactic acid bacteria on MRS agar media. Preparation of lactic acid bacteria probiotic is done by sterilizing sugar water that has been prepared mixed inoculum of lactic acid bacteria from rumen fluid. Furthermore, the utilization of lactic acid bacteria in the fermentation of rice straw silage. The parameters measured to determine the quality of the silage are the percentage of silage damage. RESULTS AND DISCUSSION. The results showed that the positive result is characterized by the growth of white lactic acid bacteria on the media for MRS. This is in accordance with Brenner et al. 2005) which showed positive result of growth of lactic acid bacteria on MRS agar. MRS media to be a selective medium of lactic acid bacteria developed by de Man, Rogosa, and Sharpe. This medium has all the nutrients needed for the growth of lactic acid bacteria. Examination of The 20th FAVA CONGRESS & The 15th IVMA Veterinary Scientific Conference (KIVNAS) - Bali Nov 1-3, 2018 | 2 characteristic of lactic acid bacteria isolated from rumen fluid was identified by Gram positive staining characterized by purple and basil-shaped lactic acid bacteria cells and coccus. Positive results are characterized by the formation of gas bubbles when the bacteria are added with H2O2 (hydrogen peroxide). Lactic acid bacteria are anaerobic but are able to tolerate the presence of oxygen. While on examination of motility test, obtained negative result (non motile). Lactic acid bacteria have characteristics as Gram positive bacteria, do not form spores, rod or round shape, catalase and negative oxidase, and have non motile properties. The percentage of rice straw silage damage was calculated based on the number of diffuse rice straw silage and the amount of rice straw silage that clumped. The result of calculation of rice straw silage riot in group P0 is 29,78%, P1 equal to 30,55%, P2 equal to 46,57%, P3 equal to 33,33%, P4 equal to 22,72%, P5 equal to 20% and P6 equal 45.45%. Based on the calculation results can be said that the damage of rice straw silage in each treatment group ranging from P0-P6 does not reach 50% of the total rice straw silage. The presence of lactic acid bacteria in silage can prevent silage from damage. REFERENCE [1] Axelsson, L. 2004, Lactic Acid Bacteria: Classification and Physiology.In Salminen, S., Wright, A.V., Ouwehand, A., editors.

Lactic Acid Bacteria: Microbiological and Functional Aspects, 3rd edition, revised.



[2] Brown, A. 2001. Microbiological Applications Lab Manual 8th Edition. The McGraw-Hill Companies, New York. [3] Ennahar, S., Cai, Y., and Fujita, Y. 2003, Phylogenetic Diversity of Lactic Acid Bacteria Associated with Paddy Rice Silage as

Determined by 16S Ribosomal DNA Analysis. Journal Appl. Environ. Microbiology 68:444-451. [4] Kaiser, A. G. and W. Piltz J. 2004, Feed Testing: Assessing Silage Quality. In: Top Fodder Successful Silage.2nd edn. Kaiser,

A.G., Piltz, J.W., Burns, H.M., and Griffiths, N.W. (eds.) NSW Department of Primary Industries and Dairy Australia, New South Wales. pp 311-334.

[5] Kurniawan, D., Erwanto dan Fathul, F. 2015, Pengaruh Penambahan Berbagai Starter Pada [6] Piltz, J. W. and Kaiser A. 2004, Principles of Silage Preservation. In: Top Fodder Successful Silage. 2nd edn. A. G. Kaiser, J. W.

Piltz, H. M. Burns, and N. W. Griffiths (eds.) NSW Department of Primary Industries and Dairy Australia. New South Wales. pp 25-56.

[7] Ratnakomala, S., Ridwan., Kartina, G., dan Widyastuti, Y. 2006, Pengaruh Inokulum Lactrobacillus plantarim 1A-2 dan IB-L terhadap Kualitas Silase Rumput Gajah ( pennisetum purpureum). Jurnal Biodiversitas. 7 (2) : 131-134.

[8] Weinberg, Z., G., Muck R., E., Weimer P., J., Chen Y., and Gamburg M. 2004, Lactic Acid Bacteria used in Silage Inoculants as Probiotics for Ruminants. Journal Applied Biochemistry Biotechnology. 118 : 1-10.



P – 08

The Applification Of Barcode Dna Rbcl Gen For Identification Of Medicinal Plants: Red Jabon And Gofasa

Sundari1, Khadijah2, A.M Jayali2, N.H Sukamto3

1*) Departement Biology education, Faculty of Teacher and Training education, Khairun University Ternate,Indonesia 2) Departement Chemistry education, Faculty of Teacher and Training education, Khairun University Ternate,Indonesia

3)Departement Chemistry, Faculty of Mathematic and Natural Science, Hasanudin University Makasar, Indonesia


INTRODUCTION Plant red Jabon and Gofasa has potential as a medicinal plant. One of the potential drugs of these two plants as anticholeterol. The purpose of this study was to identify the red Jabon and Gofasa plants in Ternate island using rbcL gene of DNA barcode. METHODS The method in this study consist of total DNA isolation using ZymoBiomic (Zymo Research DNA Extraction) Kit, DNA amplification using rbcL gene, sequencing and BLASTN analysis. Based on barcode DNA rbcL, the red Jabon plants has similarities 99% Cephalanthus occidentalis, Mitragyna speciosa and Ocherreuclear sp. For Gofasa plants has 99% resemblance with Vitex glabrata, Vitex megapotamica, Vitex trifolia and Vitex negundo at NCBI database. RESULTS AND DISCUSSION The application of rbcL barcode DNA is enough effective to identify plants at the family level up to the genus. REFERENCES [1] Consortium Barcode of Life (CBOL). (2009). A DNA Barcode for Land Plants. PNAS, 106 (31). [2] Cumming, M.P., Nugent, J.M., Olmstead, R.G. & Palmer, J.D. (2003). Phylogenetik Analysis Reveals Five Independent Transfer

of the Chloroplast Gene rbcL to the Mitochondrial Genome in Angiosperms. Curr Genet, 43:131-138. [3] Hollingsworth PM, Graham SW, Little DP (2011) Choosing and using a plant DNA barcode. PLoS ONE 6: e19254. doi:

10.1371/journal.pone.0019254 [4] Kritpetcharat O, Kritpetcharat P, Daduang J, Daduang S, Suwanrungruang K, Khemtonglang N, Bletter N, Sudmoon R,

Chaveerach A (2011) Using DNA markers and barcoding to solve the common problem of identifying dried medicinal plants with the examples of Smilax and Cissus in Thailand. Journal of Medicinal Plants Research 5: 3480–3487.

[5] Kwanda N, Noikotr K, Sudmoon R, Tanee T, Chaveerach A (2013) Medicinal parasitic plants on diverse hosts with their usages and barcodes. Journal of Natural Medicines 67: 438– 445. doi: 10.1007/s11418-012-0695-2



P – 09

Synthesis, Docking Molecule Study and Antibacterial Activity of N’-(4-Fluorobenzylidene) -4-hydroxybenzohydrazide, and

N’-(3-Bromobenzylidene) -4-hydroxybenzohydrazide

Suzana1*, Melanny Ika Sulistyowaty1, Isnaeni1 and Tutuk Budiati 1

1Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Universitas Airlangga, Surabaya 60285 [email protected]

1Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Universitas Airlangga, Surabaya 60285: [email protected]

1Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Universitas Airlangga, Surabaya 60285 [email protected]


INTRODUCTION. Many antibacterials are already resistant, so new antibacterial is needed. Objective: The compounds of N'- (4-Fluorobenzylidene) -4-hydroxybenzohydrazide, and N' - (3-Bromobenzylidene) -4-hydroxybenzohydrazide as antibacterial generally contain azometin (HN-N=CH-) and halogen groups. Before the compounds to be synthesized were done docking molecule study, to predict its activity as antibacterial. METHODS . The synthesis was carried out by microwave and identification of the results with FT-IR, 1H-NMR, 13C-NMR and MS, docking molecule study with the Molegro Virtual Docker program, and antibacterial activity by diffusion method. RESULTS AND DISCUSSION. The obtained compounds of N '- (4-Fluorobenzylidene) -4-hydroxybenzohydrazide, and N' - (3-Bromobenzylidene) -4-hydroxybenzohydrazide 87%, and 81% yield respectively. The results of docking molecule study obtained rerank score lower than the starting material (methyl 4-hydroxybenzoate). The compounds have antibacterial activity against Bacillus subtilis (Gram positive) and Escherichia coli (Gram negative). Conclusion: N '- (4-Fluorobenzylidene) -4-hydroxybenzohydrazide and N' - (3-Bromobenzylidene)-4-hydroxybenzohydrazide have been synthesized, characterized and exhibited promised antibacterial. REFERENCE [1] Mallikarjuna, B.P., Sastry, B.S., Kumar, G.V.S., Rajendraprasad, Y., Chandrashekar,

S.M.,Sathisha, K. European Journal of Medicinal Chemistry, 44, pp.4739-4746 (2009). [2] Ritesh, P.B., Kishore, P.B., Bhusari. Digest Journal of Nanomaterial and Biostructures, 4(2),

pp.329-338 (2009).



COMMITTEE

5th International Conference of Advance Molekular Bioscience & Biomedical

Engineering (5th ICAMBBE 2018)

September, 3rd-4th 2018

Steering Committee

• Rektor

• Wakil Rektor I

• Wakil Rektor II

• Prof. Dr. drh. Aulanni’am, DES

• Prof. Djoko Wahono Soeatmaji, dr, SpPD (K)

• Prof.Gugus Irianto M.SA Ph.D

Organizing Committee

Chairman of Committee • Prof. Fatchiyah, M.Kes., Ph.D

Vice Chairman of Committee • Dr. Ir. Gatot Ciptadi. DESS

Treasurer • Aisah Maulani, S.Pt

• Dwi Rachmawati, SE

Secretariat • Megawati Sistin A., S.Si

• Lidwina Faraline Tripsila, M.Si

• drh. Fitria Novitasari

• Arifudin, S.TP

Scientific Operational • Dyah Kinasih Wuragil, S.Si., MP., M.Sc

• Anna Safitri, S.Si., M.Sc., Ph.D

• Dr. Ekowati Retnaningtyas, S.Kp., M.Kes

• dr. Rulli Rosandi, SpPD KEMD

• Wahyu Nur Laili Fajri, M.Si

• Rista Nikmatu Rohmah, M.Si

• Nur Indah Ratnasari, S.Si

Event • Dr. Ir. Anik Martinah Hariati, M.Sc

• Anita Herawati, M.Si

• Perdana FInawati P, S.Si

• Dessy Wulandari EkaPutri, S.Si

Sponsorship, Publication and

Documentation

• Nia Kurniawan, S.Si.,MP.,D.Sc

• Yulianto M. Nugroho, S.Si

• Sanudi, S.Pi

Accommodation and Transportation • Akbar Farid H, S.Si

• Syahriar Andinata, S.Pt

• Bayu

Hospitality • Drh. Herlina Pratiwi, MS

• Pupimadita Tizar Afdora, M.Si

• Susiati, S.Si,.M.Biomed



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SWISS BELL INN HOTEL

ICAMBBE 2018

Swiss Bell Inn Hotel 1st Floor

LIFT

THE DINING ROOM

SWIMMING POOL ARJUNA

III

ARJUNA I

ARJUNA

II

TOILET

&

MUSHOLA

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Documents

5 th International Conference on Advance Molecular Bioscience …icambbe.ub.ac.id/wp-content/uploads/2018/09/ICAMBBE-2018.pdf · 2018-09-03 · Dr. Syafarudin, B.Eng., M.Eng. (Deputy