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5. Microbial Growth2-ST
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Microbial Growth
Chapter 6
Objectives• Describe some techniques
used to grow microorganisms• Explain the requirements for
the growth of microorganisms• Explain the different phases of
bacteria growth
Growth of Microbes• Bacteria growth is an increase in number of
cells, not cell size– One cell becomes colony of millions of cells
• Bacteria divide by – Binary fission– Alternative means
•Budding (yeast)•Conidiospores (filamentous bacteria,
actinomycetes)•Fragmentation
• Control of growth important in control of infection
Figure 6.12a
Binary Fission
Generation Time• Time required for cell to divide, for
population to double• Average for bacteria is 1-3 hours• E. coli generation time = 20 min
–20 generations (7 hours), 1 cell becomes 1 million cells!
–Exponential growth
Figure 6.12b
Cell Division and Growth Curve
Phases of Growth• Lag phase:
– making new enzymes in response to new medium
• Log phase (exponential growth):– Most sensitive to drugs
and radiation during this period
• Stationary phase:– nutrients becoming
limiting or waste products becoming toxic
– death rate = division rate
• Death phase:– death exceeds division
Standard Growth Curve
Direct Measurement of Microbial Growth
• Several methods of measuring cell growth– Direct
• Plate count• Filtration• Microscopic count• Most probable number
– Indirect• Turbidity, Metabolic
activity, Dry weight
Figure 6.16
Serial Dilutions• Reducing concentration to manageable levels• After incubation, count colonies on plates with 25–250 colonies
(CFUs)• Measures viable cells; require 24 h or longer; assume that each
bacterium produces a single colony
Counting Bacteria by Filtration
• Water sample are passed through a thin filter whose pores are 0.22 m in size
• Used to detect coliform bacteria (indicators of fecal contamination) in drinking water
Most Probable Number (MPN)• M/os being counted usually grown in liquid media
– Usually liquid differential medium is used
• You are making a statement that there is a 95% chance the bacterial population falls within a certain range
Direct Microscopic Count of Bacteria
Estimating Bacterial #s by Turbidity
•A practical way of measuring bacterial growth in a spectrophotometer
•A beam of light is transmitted through a bacterial suspension to a light-sensitive detector
•As bacterial #s increase, less light will reach the detector
•The turbidity is expressed as absorbance or optical density in a scale of 0 to 2
Ex.• A piece of hamburger meat is left at room
temperature for 2 hrs. If the generation time for pathogenic E. coli (O157:H7) is 20 min, how many bacteria would be present at the end of the 2 and 7 hrs if you only had 2 bacterium at the beginning?
• A)128• B)4,194,304