2Full Length for 8th Biomass-s

8/12/2019 2Full Length for 8th Biomass-s

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Bio-Ethanol Production Ability of Straw Mushroom Waste Bed

from Vietnamese Local Agriculture

Kouji Yoshida1, Ngo Duc Duy2, Hoang Quoc Khanh2, Kazuhiro Mochidzuki1, Yasuo Igarashi3, and

Akiyoshi Sakoda1

1Institut o! Industria" Scinc, th #ni$rsity o! %okyo, &'('1 Ko)a*a, Mguro'ku, %okyo 1+3'

+-+, .a/an

2Institut o! %ro/ica" 0io"ogy, itna) Acad)y o! Scinc and %chno"ogy, (21 Ha Noi High4ay,

5inh %rung 6ard, %hu Duc Dist7, Ho 8hi Minh 8ity, itna)

39raduat Schoo" o! Agricu"tura" and 5i! Scincs, %h #ni$rsity o! %okyo, 1'1'1, Yayoi, 0unkyo'

ku, %okyo, 113'(+:

Ky4ords; stra4 )ushroo), 4ast *d, *io)ass, *io'thano", "oca" agricu"tur

8ontact addrss< a)ati=iis7u'tokyo7ac7j/

Abstract:

%h stra4 )ushroo) >Volvariella volvacea? is gro4n on th ric stra4 4hich is on o! th

"argst so!t *io)ass in itna)7 6 4r ins/ctd and co""ctd )ushroo) *d sa)/" !ro) %an

Hoa i""ag, 5ai ung, Dong %ha/ @ro$inc, in Mkong D"ta7 ic stra4 4as trans/ortd *y cana"s,

co)/ostd in 2 4ks, ridg !or)d and inocu"atd th )ushroo), thn k/t undr high )oistur

condition in 2 4ks7 6 co""ctd 3 o! di!!rnt )ushroo) *d sa)/"s, non')ushroo)'cu"ti$atd

stra4 !or contro"s, thn )asurd !i*r contnts and in$stigatd !r)ntation a*i"itis7 %h !i*r

ana"ysis r$a"d that ach )ushroo) *d sti"" containd nough high a)ount o! c""u"os7

Br)ntation a*i"ity 4as $a"uatd *y Si)u"tanous Sacchari!ication and Br)ntation>SSB? using

c""u"as and yast7 Cn 4k SSB cou"d /roduc a !4 a)ount o! thano" !ro) th )ushroo) *d7

ntroduction

%h rsarch da"s 4ith $a"uations !or *io)ass usag o! *y/roduct !ro) itna)s "oca"

agricu"tur syst), undr a rsarch /rojct Sustaina*" Intgrations o! 5oca" Agricu"tur and

0io)ass IndustrisE >SA%F@S, .I8A'.S%?7 %h /rojct ai)s to construct sustaina*" agricu"tur

socity using "oca" *io)ass uti"ization7 %o ra"iz this /ur/os, uti"ization a*i"itis o! *io)asss 4r$a"uatd7 0caus o! th high"y ric /roduction in itna), 4 ar !ocusing ric stra4 as th /art o!

th "oca" *io)ass7

Stra4 )ushroo) >Volvariella volvacea? 4ast *d 4as intrstd in as on o! stra4 *asd

*io)ass in itna); thr!or thano" /roduction a*i"ity !ro) th 4ast *d 4as $a"uatd7 Stra4

)ushroo) is 4id"y cu"ti$atd !ro) southrn china to South Fast Asia, inc"uding itna)7 Nor)a""y

di*" )ushroo) rGuirs 4oody )atria" to cu"ti$at, ho4$r stra4 )ushroo) can * gro4n on th

grass )atria", inc"uding ric stra47 @r$ious r/ort /ointd out that stra4 )ushroo) /roduction

cou"d accu)u"at c""u"os contnt and dcras "ignin contnts, thr!or )ushroo) cu"ti$ation

cou"d * *io"ogica" /rtrat)nt !or ric stra4 >1?7 Ho4$r, stra4 )ushroo) is a"so kno4n to *


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c""u"ash)ic""u"as /roducr, thr!or, th )ushroo) can dgrad c""u"os >2?7 Birst 4 trid to

gras/ thano" /roducti$ity o! th )ushroo) *d7

Straw mushroom culti!ation in "an #oa Village

%h stra4 )ushroo) !ar)ing in %an Hoa i""ag, 5ai ung, Dong %ha/ @ro$inc, in Mkong

D"ta, 4as ins/ctd in 23 thto 2&th,.u"y and thAugst, 2-117

%h stra4 d"i$rd !ro) )arkt *y cana"s using *oat >!ig71?, and /i"d u/ )ountain >!ig73?7

%h stra4 )ountain 4as 4"" draind 4ith cana" 4atr and k/t 2 4ks !or !r)ntati$

/rtrat)nt >!ig73?7 During d"i$ring and !r)ntati$ /rtrat)nt tr)s, th stra4 4as hatd u/

*y !r)ntation and th sta) 4as o*sr$d >!ig72?7 A!tr !r)ntation /rtrat)nt, th stra4 4as

)ad to "an undr !ruit tr >7g7, "ongan, !ig7&? or co$rd 4ith drid stra4 >!ig7(? to k/ th

)oistur, and thn th sd )ushroo) 4as inocu"atd7 A!tr 2 4ks cu"ti$ation, )ushroo) 4as

har$std and th *d 4as co""ctd to us as th !rti"izr o! th !"o4r /roduction7

Figure 3, Delivered rice straw was piled up

to mountain and drained by canal water for

2 weeks fermentative pretreatment.

Figure 4, Pretreated straw was made to

lane and then straw mushroom seed

culture was inoculated. Trees were longan.

Figure 5, Sampling point of around

longan tree mushroom bed. Mushroom

grew well around sampling point.

Figure 6, Sampling point of river side

mushroom bed. Cover straw also sampled

as control.


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Materials and methods

Sa)/"s

Mushroo) *d sa)/"s !ro) ri$r sid *d >!ig7(?, around "ongan tr *d >!ig7&,+? and

attachd on th har$std )ushroo) 4r co""ctd to ana"ysis7 %h stra4 usd to co$r th

)ushroo) *d >!ig7(? and a!tr /rtratd >i77, *!or )ushroo) inocu"u)? stra4 a"so co""ctd as

contro"7 %h *d sa)/"s 4r co""ctd !ro) /oints that )any )ushroo)s can * sn7

Bi*r ana"ysis

%h !i*r contnts 4r )asurd *y su"!uric acid hydro"ysis )thod >3?7 Sa)/"s 4as drid

u/ at -in 2 or 3 days, and )i""d to undr 1))7 -73g o! ach sa)/" 4as /ut into th )diu) jar

>1-- )"?, 3)" o! :2>$$? su"!uric acid so"ution 4as addd to th jar and thn th jar k/t in th 4atr

*ath >3-? !or 1 hour 4ith stirring >thick acid trat)nt?7 A!tr thick acid trat)nt, th acid 4as

di"utd to & >$$? thn k/t in autoc"a$ >121? !or 1 hour >thin acid trat)nt?7 A!tr thin acid

trat)nt, so"id !raction 4as !i"tratd *y 9ooch cruci*" >/r'*urnd and 4ighd, /or siz


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4as /r!or)d 4ith 3>dry 4$? o! ach sa)/" >)i""d in 4t condition?, +>44 dry *io)ass? o!

c""u"as >Acr)oniu) c""u"as, Miji? and 1>4$? o! /o4drd corn st/ "iGuor >So"u"ys -+F,

ockt?7 %h 4orking $o"u) o! ach SSB 4as 1--)", sa"d 4ith air "ock /"ug, th SSB 4as

incu*atd at 3+ 4ith stirring at 1+-r/)7 %h concntration o! r"asd g"ucos and !r)ntd

thano" 4r )asurd *y H@58 >Shi)adzu? 4ith Sugar 8o"u)n >ShodJ Sugar 8o"u)n? and

dtctd *y r!racti$ indJ dtctor >Shi)adzu?7

No any othr /rtrat)nt 4as /r!or)d on th sa)/"s7

$esults and %iscussions

%h !i*r contnts o! ach sa)/" 4r sho4n in !igur (7 8ontro" >i77, co$r stra4? contains

&- o! c""u"osstarch and 33 to 3& o! c""u"osstarch sti"" r)ains in th )ushroo) *d7 5ignin

contains cou"d not * rducd during )ushroo) !ar)ing, n$rth"ss th /r$ious r/ort /ointd out

th /ossi*i"ity o! "ignin dgradation a*i"ity o! th stra4 )ushroo) >1?7 Stra4 )ushroo) has $ry

short gro4th tr), nor)a""y on"y 24ks, co)/aring 4ith othr di*" )ushroo)s, nor)a""y o$r

1 )onth7 It 4as considra*" that th nons"cti$ dgradation a*i"ity cou"d su//ort that Guick gro4th

Figure 6

Fiber contents of cover straw, pretreated bed and each straw mushroom bed sample.

Straw mushroom did not show selective lignin degradation.


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o! th )ushroo)7

Si)u"tanous sacchari!ication and !r)ntation 4as /r!or)d to $a"uat thano" /roduction

a*i"ity o! )ushroo) *d sa)/" >!ig7 :?7 Mushroo) *d sa)/"s !ro) around "ongan tr, ri$r sid

and co$r stra4 sa)/" 4r a//"id to SSB7 "asd g"ucos and /roducd thano" concntration

4as con$rtd to yi"d >? $rsus thortica" g"ucosthano" /roducti$ity that ca"cu"atd !ro)

c""u"osstarch contnts o! ach sa)/" >!ig7(?7 A"though, no othr /rtrat)nt >7g7, a"ka"in or

acid trat)nt?, thano" /roducti$ity o! co$r stra4 4as sti"" high; ho4$r, thano" yi"d o! ach

sa)/" 4as "o4r than contro" stra4 >i77, co$r stra4?7 %his 4ou"d * considrd that asy'to'accss

c""u"os 4as a"rady usd *y )ushroo)7 "asd g"ucos concntration !ro) ach sa)/" 4as

a")ost sa) during SSB /riod, ho4$r, thano" /roducti$ity 4as di!!rnt7 %h g"ucos

concntration 4as r"atd to yast a*i"ity and yast a*i"ity 4as k/t nough high during th /riod,

thr!or, th concntration 4as k/t $ry "o47

A"though th *d sti"" r)aind *io'thano" /roducti$ity, !urthr )or in$stigations shou"d *

rGuird to us stra4 )ushroo) *d !or *io'thano" rsourc7


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Ackn

o4"dg)nt

%his 4ork 4as su//ortd *y .S%.I8A'SA%F@S, Sustaina*" Intgration o! 5oca"

Agricu"tur and 0io)ass Industris7 Mr7 Nguyn 8ong Man kind"y introducd us to %an Hoa i""ag,

5ai ung, Dong %ha/ @ro$inc7

1? .ustin 07 S"uitr, ay)ond C7 uiz, 8hristo/hr .7 Scar"ata, A)i D7 S"uitr, and Da$id 67%)/"ton, 8o)/ositiona" Ana"ysis o! 5ignoc""u"osic Bdstocks7 $i4 and Dscri/tion o!

Mthods,J Agric Food Chem7 2+; +>1(?< /7-&3L-+3 >2-1-?

2? Y7 .7 8ai, .7 A7 0us4"" and S7 %7 8hang, @roduction o! c""u"ass and h)ic""u"ass *y th stra4

)ushroo), o"$ari""a $o"$aca,Mycal. Res. >?, 1-1'1-2& >1&?

3? A7 @hi"i//oussis, 97 r$akis and @7 Dia)anto/ou"ou, 0iocon$rsion o! agricu"tura" "ignoc""u"osic

4ast through th cu"ti$ation o! th di*" )ushroo)sAgrocybe aegerita, Volvariella volvaceaand

Pleurotuss//7 World J. Microbiol.Biochem.1:< /711'2-- >2--1?

Figure 7

SSF results of cover straw river side bed sample (triangle) and longan bed sample

(circle).Open symbols showed glucose yield and filled symbols showed ethanol yield from

each sample.

Documents

2Full Length for 8th Biomass-s