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CURRICULUM VITAE

Chung-Tiang (David) Liang D.V.M., M.S., Ph.D.

Veterinarian Department of Animal Facility

Novo Nordisk Research Centre China

Building #2, 20 Life Science Park Road Changping District

Beijing 102206 China

+86 10 53211208 (direct) 86 1381156-6542 (mobile)

znlg@novonordisk.com

EDUCATION EXPERIENCE

Johns Hopkins University, School of Medicine, Baltimore, Department of Molecular

and Comparative Pathobiology, Rodent Phenotyping Core USA

Post Doctorate /Visiting Scientist, 2010

National Taiwan University, Department and Graduate Institute of Veterinary

Medicine, School of Veterinary Medicine Taiwan

PhD. , Major (Veterinary Pathology and Laboratory Animal Medicine), 2011

National Taiwan University, Department of Veterinary Medicine Taiwan

MS. , Major (Veterinary Pathology), 1988

National Taiwan University, Department of Veterinary Medicine Taiwan

DVM. , 1985

PROFESSIONAL EXPERIENCE

AAALAC ad hoc Site Visitor

09/2014~08/2017 Asia, Pacific Rim

I have been the AAALAC ad hoc specialist (site visitor), since Sep. 2014 to Aug. 2017. I have been the

IACUC member of five institutes, including National Laboratory Animal Center (NLAC) (Taipei and

Tainan branch), National Health Research Institute (NHRI), Fu Jen Catholic University and National Cheng-

Kung University. I did quite a lot of protocol review annually, 20 to 30 cases per year for 10 years. I also did

so many internal semi-annual site visiting and IACUC meeting in these facilities.

Site Visitor

07/2004~now Taiwan

I engaged and as a site visitor of a project titled” Nationwide site visit for 40 animal facilities annually”

grant supported by CSLAS and Council of Agriculture, Taiwan. Each time, we will send two to three

experts to do the site visit of animal facilities. If anything not compatible with animal welfare or well-being,

we will tell them (IACUC) to correct immediately. We also do the annual evaluation of these 40 animal

facilities by groups of expert with standard semi-quantitative scores to check which facility is excellent and

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which is not good for animal use and care. If some facility did not follow our suggestion, we will do the site

visit next year till them correct the deficiency of animal care and use program. I have been the member of

the Chinese Taipei Society of Laboratory Animal Sciences (CSLAS) for fifteen years and recently been the

“secretary general” of this society for four years (2012 till present). The Chinese Taipei Society of

Laboratory Animal Science, a scientific association for laboratory animal science, was founded in 1989 with

an aim to promote the quality and wellbeing of laboratory animals. In addition, the Animal Protection Act,

which governs the scientific application of animals, was approved by the Taiwanese government in 1998.

Associate Researcher/Attending Veterinarian Taiwan

10/1991~now

I received a DVM degree from National Taiwan University, Department of Veterinary Medicine

(NTUDVM) in 1985. I also spent 3 years in NTUDVM to finish MS degree in 1988. My title of MS

thesis is “Pathological studies of abortion, stillbirth and neonatal deaths on a swine herd in Taiwan”. One

paper of my MS thesis titled” Congenital abnormalities in piglets from a purebred swine herd “got the 1995

National Science Council annual project award. During my MS period, I also completed three year

veterinary anatomy pathology residency training in Pig Research Institute, Taiwan (PRIT) under the

supervision of Dr. Redman RM Chu, and Dr. Chen-Hsuan Liu et al. When I graduated, I went to military

army for two years (July 1988 to May 1990). Then after short term job in local pet animal clinic, I took the

exam and interview, and become the key person of National Laboratory Animal Center (NLAC) since Oct

1991. I worked in the NLAC more than 24 years since Oct 1991. I also got promotion as the attending

veterinarian (AV) of NLAC (Taipei branch) from 2002 till present. As the AV, I did the IACUC protocol

preview of each case. I supervises laboratory animal care and use (including Health Monitoring Program,

Animal Quarantine, Animal Health Abnormality Handling), daily runs veterinary inspection, daily-animal

rooms observation record reviewing, supervises health monitoring diagnosis including diagnosis records

keeping, quarterly health monitoring report announcement, controlled drugs management, internal and

external education and training and surgical authorization.

As a key person in NLAC, NLAC received full accreditation from the Association for Assessment and

Accreditation of Laboratory Animal Care International (AAALAC International) in 2007, 2010 and 2013;

the accreditation indicates that NLAC has met the criteria in excellent animal management and use program.

My specialty is veterinary pathology, immunohistochemistry, toxicological pathology, animal tumor

diagnosis, rodent pathology phenotyping of genetic modified (GM) rodents and laboratory animal medicine.

I am also in charge of the first and second” International pathology phenotyping of GM mice workshop in

Taiwan” in 2009 and 2011. Between that period, I went to Jones Hopkins University, school of medicine,

phenotyping center, Baltimore, to take five months training of veterinary pathology, pathology phenotyping

and laboratory animal comparative medicine training (July 28- Dec 18, 2010). In that period, I also

completed my Ph.D. degree in 2011. My doctoral dissertation titled” Optimization of the in situ detection of

viral protein and nucleic acid in tissue: mouse hepatitis virus and canine distemper virus". The training program/course abroad or in Taiwan I have attended including:

1. American veterinary pathologist and veterinarian residency training, (Baltimore, Jones Hopkins Medical

School, phenotyping core), 2010 (July 28, 2010-Dec 18, 2010).

2. The Rodent Pathology in Translational Research and Phenotyping Workshop, Taiwan (Coordinated and

leaded by Johns Hopkins University, School of Medicine , Baltimore, Rodent Phenotyping Core, Dr.

Cory Brayton), 2011.

3. Endocrine Pathology, Seventh RTP Rodent Pathology Course, Cary, North Carolina, USA, 2014.

4. Hepatobiliary Pathology, Fifth RTP Rodent Pathology Course, Cary, North Carolina, USA, 2010.

5. AALAS 60 th national meeting, Denver, Colorado, USA, 2010

a) Preparing for an AAALAC international site visit

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b) Jejular vein catheterization in the rat

6. AMMRA/AMPC annual meeting and workshop on mouse model research, Nanjing, 15-17th, 2012

a). Status report from NLAC phenotyping centers (short presentation)

b). Introduction of pathology phenotyping in GM mice (short presentation)

7. Kumamoto University and Riken BioResource Center, Riken BRC phenotyping facility visit, 2012.

8. Infrafrontier/IMPC Korea Meeting Program, Korea, 2012.

9. Mary Lyon Centre, MRC Harwell and Welcome Trust Sanger Institute phenotyping facility visit, 2012.

10. IMPC Toronto TCP phenotyping meeting and visit, 2012.

11. AALAS 64 th

. national meeting, Baltimore, USA, 2013.

a).Animal facility design, process, design and technology

b).Tools for performing and training aseptic techniques for rodent surgery.

12. Preparing for an AAALAC international site visit course, The Chinese society of laboratory animal

sciences, Taiwan, 2011.

13. Laboratory animal breeding training, NIH, Washington DC, USA, April to July, 1992.

14. Laboratory animal medicine and health monitoring training, Jackson Laboratory, Bar Harbor, Maine,

Jan to March,1992.

15. Charles River Laboratories training, training and experiences in laboratory animal health monitoring and

clinical diagnosis, Maine, USA, 1993.

16. Armed Force institute of Pathology, trainings and experiences in histopathology diagnosis,

immunochemical staining, surgical and clinical pathology, Washington DC, USA, 1996.

17. Training course on the humane care and use of laboratory animals, Council of Agriculture, Taiwan

18. Pathology of Genetically Engineered Rodents and Aquatic Species, an Introduction to Phenotyping

Workshop, Covance Laboratory Learning Center, USA, 2007.

19. 7th Annual Workshop on the Pathology of Mouse Models for Human Disease. Cornell University,

Ithaca, NY, 2008.

20. The Rodent Pathology Phenotyping Workshop, Taiwan (Coordinated and leaded by Johns Hopkins

University, School of Medicine , Baltimore, Rodent Phenotyping Core, Dr. Cory Brayton), 2009.

TEACHING EXPERIENCE

1. Laboratory Animal Medicine (Health monitoring program, euthanasia, anesthesia, post-surgery operation

care, bleeding and restraint techniques, humane endpoint, animal welfare and daily veterinary inspection)

2. Veterinary Pathology (Swine disease, exotic animal, dog and cats disease)

3. Mice and rats necropsy and tissues trimming

4. Mice pathology phenotyping and slides interpretation

5. Mice bone marrow extraction and smear interpretation

6. Laboratory Animal Disease (Infectious, non-infectious and neoplastic disease, GEM pathologic

phenotyping)

7. Veterinary histology (chiefly including mice, rats, swine, canine, feline, guinea pigs, rabbits, hamster,

and wild animals)

8. Molecular pathology and related techniques (IHC, ISH, IFA, RT-PCR, cell culture, virus isolation,

phylogenetic analysis)

AWARD

1. 2009 Taiwan Vet J annual best paper award” Isolation and the phylogenetic analysis of canine distemper

viruses in Taiwan. Taiwan Vet J 34(4), 198-210, 2008. “

2. 1995 National Science Council annual project award” Congenital abnomalities in piglets from a purebred

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swine herd. J Chin Soc Vet Sci 21：183-195, 1995”, USD 3,000

3. 1997 National Science Council annual project award” The occurrences of spontaneous tumors in SFP rats

and mice. J Chin Soc Vet Sci 23：531-545, 1997.”, USD 3,000

4. 1998 National Science Council annual project award” Case report: Histiocytic Sarcoma in a mouse. J

Chin Soc Vet Sci 23：506-514, 1997.”, USD 3,000

PUBLICATIONS & ACADEMIC PRESENTATIONS

1. Liu YH, Tsai YS, Lin SC, Liao NS, Jan MS, Liang CT, Hsu SW, Chen WC, Sung JM, Maeda N, Tsai

PJ. Quantitative PPARγ expression affects the balance between tolerance and immunity. Scientific

Reports, 6:26646, DOI: 10.1038/srep26646, 2016 ((SSCCII))

2. Chang HM, Lin YY, Tsai PC, Liang CT, Yu-Ting Yan YT. The FYVE domain of smad anchor for

receptor activation (SARA) is required to prevent skin carcinogenesis, but not in mouse development

PLOS One 9(8), e105299. doi:10.1371/journal.pone.0105299, 2014 ((SSCCII))

3. Canene-Adams K, Sfanos KS, Liang CT, Yegnasubramanian S, Nelson WG, Brayton C, De Marzo AM.

Dietary chemoprevention of PhIP induced carcinogenesis in male Fischer 344 rats with tomato and

broccoli. PLOS One 8(11), e79842, 2013 ((SSCCII))

4. Chang TK, Ho P, Liang CT, Yu CK. Vaginal septa decrease the reproductive performance of

BALB/cByJNarl mice. J Amer Assoc Lab Anim Sci, 52(5): 520-523, 2013 ((SSCCII))

5. Liang CT, Chueh LL, Pang VF, Lee KH, Liang SC, Lee CC, Lee YH, Liu CH. Improving detection of

canine distemper virus in formalin-fixed, paraffin-embedded tissues: using in situ hybridization with

integrated optical density to give a semi-quantitative assessment. J Appl Res Vet Med. 10(4): 335-343,

2012 (SCI)

6. Hsieh AAHH,, Jhoouu YYJ, Liang CT, Chang MI, Wang SL. Fragment of tegument protein pp65 of human

cytomegalovirus induces autoantibodies in BALB/c mice. Arthritis Research & Therapy,, 13(5):R 1162 :1-

15, 22001111.. (SSCCII))

7. Liang CT, Chueh LL, Brayton C, Pang VF, Wu SC, Huang SW, Liang SC, Yu CK, Lee CC, Liu CH.

Canine Distemper in Taiwan from 2000 – 2009: co-infections, and use of RT-PCR and

immunohistochemistry to detect tiisssue involvement in ttwwoo ggrouuppss of dogs. Inter J Appl Res Vet Med 9(3):

265-277, 2011. (SSCCII))

8. Huang SW, Ho PY, Cheng PH, Ma WH, Chen YL, Liao SL, Chiu YY, Yu CK, Liang SC, Liang CT.

Case report: High mortality associated with ketorolac treatment in CByB6F1 mice after embryo transfer

surgery. Taiwan Vet J 36(4): 261-268, 2010.

9. Liang CT, Shih A, Chang YH, Liu CW, Huang YL, Huang WT, Kuang CH, Lee KH, Zhuo YX, Ho SY,

Liao SL, Chiu YY, Hsu CN, Liang SC, Yu CK. Microbiological contamination of laboratory mice and

rats in Taiwan from 2004 to 2007. J Amer Assoc Lab Anim Sci 48(4): 381-386, 2009 (SCI)

10. Zhuo YX, Liang CT, Lin YH, Chen JF, Tseng YS, Liao HL, Liang SC, Huang YT. Retrospective study

of spontaneous lesions in the germfree mice and rats. Taiwan Vet J 35(2): 124-130, 2009. (in Chinese)

11. Liang CT, Chueh LL, Lee KH, Huang HS, Takehara K, Miura R, Kai C, Liang SC, Yu CK, Liu CH.

Isolation and the phylogenetic analysis of canine distemper viruses in Taiwan. Taiwan Vet J 34(4), 198-

210, 2008.

12. Liang CT, Chueh LL, Pang V F, Zhuo YX, Liang SC, Yu CK, Chiang H, Lee CC, Liu CH. A non-biotin

polymerized horseradish peroxidase method for the immunohistochemical diagnose of canine distemper

virus infection. J Comp Pathol113366::5577--6644,, 22000077.. ((SSCCII))

13. Liang CT,, LLeeee PPCC,, WWuu SSCC,, HHuuaanngg YYTT,, CChhaanngg WWJJ,, HHsscc TTYY,, LLiiaanngg SSCC.. EEffffeeccttiivvee eerraaddiiccaattiioonn ooff ppiinnwwoorrmm

iinnffeeccttiioonn（（SSyypphhaacciiaa mmuurriiss,, SSyypphhaacciiaa oobbvveellaattaa）） ffrroomm aa llaarrggee rrooddeenntt bbrreeeeddiinngg cceenntteerr.. TTaaiiwwaann VVeett JJ

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3300::110066--111155,, 22000044..

14. Liang CT, Wu SC, Huang Y T, Lin YC, Chang WJ, Chou JY, Liang SC, Liu CH. Immunohistochemical

diagnosis of mouse hepatitis virus and Mycoplasma pulmonis infection with murine antiserum. J Comp

Pathol 113311::221144--222200,, 22000044.. ((SSCCII))

15. Liang CT, Wang HC, Liu CH. A modified immunohistochemical staining method employed in formalin

or alcohol-fixed, paraffin-embedded porcine tissue sections. J Chin Soc Vet Sci 26：213-222,2000. (in

Chinese)

16. Liang CT, Chang MH, Hong CC, Huang KJ. Establishing the blood chemistry reference valves for SPF

rats and mice. J Chin Soc Vet Sci 25:: 55-68,1999. (in Chinese)

17. Liang CT, Liu CH, Huang YT, Victor Pang F, Hans HC Chen, Hong CC. The occurrences of

spontaneous tumors in SFP rats and mice. J Chin Soc Vet Sci 23：531-545, 1997.(in Chinese)

18. Huang YT, Chang YH, Liang CT, Chou CK, Hong CC, and Wu MF, Immunological and ultrastructural

characteristics of T-cell lymphoblastic lymphoma (associated with mast cell infiltration in a Wistar rat.

Lab Anim Sci 47:209-212, 1997. (SSCCII))

19. Liang CT, Liu CH, Huang YT, Pang VF, Hong CC, Chen HHC. Case report: histiocytic sarcoma in a

mouse. J Chin Soc Vet Sci 23:506-514, 1997.

20. Chen HHC, Yu C, Ueng TH, Liang CT, Chen BJ, Hong CC, Chiang LY. Renal effects of water-soluble

polyarylsulfonate C60 in rats with an acute toxicity study. Fullerene Sci Tech5:1387-1396,1997 (SSCCII))

21. Liang CT, Chu RM, Liu CH, Chen WF, Weng CN, Liu RS. Pathological studies of abortion, stillbirth

and neonatal deaths on a swine herd in Taiwan. J Chin Soc Vet Sci 22: 270-280, 1996. (in Chinese)

22. Chen HC, Liang CT, Hong CC, Huang YT, Pan IJ. Spontaneous pulmonary squamous cell carcinoma in

an aging CD rat. Vet Pathol 33：228-230, 1996.(SSCCII))

23. Liang CT, Chu RM, Chen WF, Weng CN, Liu RS. Congenital abnomalities in piglets from a purebred

swine herd. J Chin Soc Vet Sci 21:183-195, 1995.

24. Chen HC, Pan IJ, Liang CT, Hong CC. Nasal adenocarcinoma with myoepithelial component in a CD-1

mouse. Vet Pathol 32:710-713 ,1995.( SSCCII ))

25. Liang CT. Animal models of human diseases in Taiwan. Science Bulletin 26:1-4, 1994.

26. Liu CH, Jeng CR, Chu RM, Liang CT, Yang PC. Histopathological analysis of swine pseudorabies: a

retrospective study of 82 cases (1984-1987). J Chin Soc Vet Sci 14: 219-227, 1988.

27. Chu RM, Liang CT. Lymphocytic chemotaxis of mammary gland I.the development of a multiple-wells

method for lymphocytic chemotaxis. J Chin Soc Vet Sci 13: 181-187, 1987.

28. Cheng CR, Liu CH, Liang CT, Tsai MC。Pathological study of polyarteritis nodosa in swine, six cases.

J Chin Soc Vet Sci 14：245-252, 1988. (in Chinese)