75
INTERNATIONAL TROPICAL TIMBER ORGANIZATION ITTO PROJECT PROPOSAL TITLE: GENETIC CONSERVATION, UTILIZATION AND MANAGEMENT OF GALL RUST RESISTANT STRAINS OF FALCATARIA MOLUCCANA GROWING IN THE PHILIPPINES SERIAL NUMBER: PD 771/15 Rev.2 (I) COMMITTEE: FOREST INDUSTRY SUBMITTED BY: GOVERNMENT OF THE REPUBLIC OF THE PHILIPPINES ORIGINAL LANGUAGE: ENGLISH SUMMARY: In the Philippines the demand for wood and its derived products is increasing, while areas devoted to tree plantations are shrinking. Thus, there is an urgent need to plant trees that are fast growing, with valuable utility traits, and are adapted to marginal environment. However, establishment of forest tree plantations has been hampered by the limited access to planting materials of high quality and yield potential. Biotechnology offers solutions to hasten tree improvement through advanced breeding strategies and efficient nursery and in vitro vegetative propagation methods. One industrial tree plantation species planted throughout the Philippines that requires biotechnological inputs is Falcataria moluccana. However, growth and yield of plantations of F. moluccana were low, mostly due to poor genetic selection of seed sources and poor silvicultural practices. They have also been attacked by the gall rust pathogen, but there are trees found resistant that are widely grown in the CARAGA region in Mindanao, southern Philippines. This program aims to conserve the disease resistant and plus trees of F. moluccana from the region and mass propagate them in order to increase the supply of superior quality planting materials and yield of plantations, and thereby generate better economic and environmental gains from plantations of the species. EXECUTING AGENCY: Department of Biological Sciences (FBS) College of Forestry and Natural Resources (CFNR) University of the Philippines Los Banos (UPLB) Department of Environment and Natural Resources (DENR)-CARAGA Centre de Coopération Internationale en Recherche Agronomique pour le Développement (CIRAD) DURATION: 36 MONTHS BUDGET AND PROPOSED SOURCES OF FINANCING: SOURCE CONTRIBUTION IN US$ ITTO 1,248,913 Government of the Republic of the Philippines 162,300 TOTAL 1,411,213

ITTO · 2015. 10. 6. · international tropical timber organization . itto. project proposal . title: genetic conservation, utilization and management of gall rust resistant strains

  • Upload
    others

  • View
    1

  • Download
    0

Embed Size (px)

Citation preview

Page 1: ITTO · 2015. 10. 6. · international tropical timber organization . itto. project proposal . title: genetic conservation, utilization and management of gall rust resistant strains

INTERNATIONAL TROPICAL TIMBER ORGANIZATION

ITTO

PROJECT PROPOSAL

TITLE: GENETIC CONSERVATION, UTILIZATION AND MANAGEMENT OF GALL RUST RESISTANT STRAINS OF FALCATARIA MOLUCCANA GROWING IN THE PHILIPPINES

SERIAL NUMBER: PD 771/15 Rev.2 (I)

COMMITTEE: FOREST INDUSTRY

SUBMITTED BY: GOVERNMENT OF THE REPUBLIC OF THE PHILIPPINES

ORIGINAL LANGUAGE: ENGLISH

SUMMARY: In the Philippines the demand for wood and its derived products is increasing, while areas devoted to tree plantations are shrinking. Thus, there is an urgent need to plant trees that are fast growing, with valuable utility traits, and are adapted to marginal environment. However, establishment of forest tree plantations has been hampered by the limited access to planting materials of high quality and yield potential. Biotechnology offers solutions to hasten tree improvement through advanced breeding strategies and efficient nursery and in vitro vegetative propagation methods. One industrial tree plantation species planted throughout the Philippines that requires biotechnological inputs is Falcataria moluccana. However, growth and yield of plantations of F. moluccana were low, mostly due to poor genetic selection of seed sources and poor silvicultural practices. They have also been attacked by the gall rust pathogen, but there are trees found resistant that are widely grown in the CARAGA region in Mindanao, southern Philippines. This program aims to conserve the disease resistant and plus trees of F. moluccana from the region and mass propagate them in order to increase the supply of superior quality planting materials and yield of plantations, and thereby generate better economic and environmental gains from plantations of the species. EXECUTING AGENCY:

Department of Biological Sciences (FBS) College of Forestry and Natural Resources (CFNR) University of the Philippines Los Banos (UPLB) Department of Environment and Natural Resources (DENR)-CARAGA Centre de Coopération Internationale en Recherche Agronomique pour le Développement (CIRAD)

DURATION: 36 MONTHS

BUDGET AND PROPOSED SOURCES OF FINANCING:

SOURCE

CONTRIBUTION IN US$

ITTO 1,248,913

Government of the Republic of the Philippines

162,300

TOTAL 1,411,213

Page 2: ITTO · 2015. 10. 6. · international tropical timber organization . itto. project proposal . title: genetic conservation, utilization and management of gall rust resistant strains

TABLE OF CONTENTS Project brief ....................................................................................................................................................... ii List of abbreviation and acronyms ................................................................................................................ iii Map of Project site ........................................................................................................................................... iv Part 1: Project Context .................................................................................................................................. 1

1.1 Origin .................................................................................................................................................. 1 1.2 Relevance ........................................................................................................................................... 1

1.2.1 Conformity with ITTO objectives .............................................................................................. 1 1.2.2 Relevance to the policies of Philippines ................................................................................... 2

1.3 Target area ......................................................................................................................................... 2 1.3.1 Geographical location .............................................................................................................. 2 1.3.2 Social cultural, economic and environmental aspects ............................................................. 2

1.4 Expected outcomes at project completion ......................................................................................... 3 Part 2: Project Rationale and Objectives ................................................................................................... 4

2.1 Rationale ............................................................................................................................................ 4 2.1.1 Institutional set-up and organizational issues .......................................................................... 4 2.1.2 Stakeholder analysis ................................................................................................................ 4 2.1.3 Problem analysis ...................................................................................................................... 8 2.1.4 Logical framework matrix ....................................................................................................... 12

2.2 Objectives ......................................................................................................................................... 14 2.2.1 Development objectives and impact indicators ...................................................................... 15 2.2.2 Specific objectives and outcomes indicators ......................................................................... 15

Part 3: Description of Project Interventions ............................................................................................. 16

3.1 Output and activities ......................................................................................................................... 16 3.1.1 Outputs ................................................................................................................................... 16 3.1.2 Activities ................................................................................................................................ 16

3.2 Implementation approaches and methods ....................................................................................... 16 3.3 Work plan ......................................................................................................................................... 19 3.4 Budget .............................................................................................................................................. 20

3.4.1 Master budget by activity ........................................................................................................ 20 3.4.2 Consolidated budget by component (in US$)………………………………………………...27 3.4.3 Consolidated budget by component to be requested from ITTO ................................... 29 3.4.4 Consolidated budget by component to be requested from Executing Agency ............ 29

3.5 Assumptions, risks, and sustainability .............................................................................................. 30

Part 4: Implementation Arrangements ..................................................................................................... 31 4.1 Organization structure and stakeholders involvement mechanism .................................................. 31

4.1.1 Executing agency and partners ............................................................................................. 31 4.1.2 Project management team ..................................................................................................... 31 4.1.3 Project steering committee .................................................................................................... 32 4.1.4 Stakeholders involvement mechanisms ................................................................................. 33

4.2 Monitoring, review, and evaluation ................................................................................................... 33 4.3 Dissemination and mainstreaming of project learning ..................................................................... 34

4.3.1 Dissemination of project results ............................................................................................. 34 4.3.2 Mainstreaming project learning .............................................................................................. 34

Annex 1: Profiles of the executing agencies ........................................................................................... 35 Annex 2: Task and Responsibilities of key personnel ........................................................................... 37 Annex 3: Terms of references of personnel and consultants .............................................................. 38 Annex 4: Overall assessment and specific recommendations of the 49th Expert Panel ................... 39 Annex 5: Overall assessment and specific recommendations of the 50th Expert Panel ................... 43 Annex 6. Curriculum Vitae of the /Program Coordinator………………………………………………… 46 Supplementary Material 1. Organizational Structure of the UPLB Foundation, Inc……………………. 47 Supplementary Material 2.1-2.5. MOA between UPLB and UPLBFI as separate attachment in pdf file Supplementary Material 3. UPLBFI Securities and Exchange Commission certification as separate

attachment in pdf file

-i-

Page 3: ITTO · 2015. 10. 6. · international tropical timber organization . itto. project proposal . title: genetic conservation, utilization and management of gall rust resistant strains

PROJECT BRIEF

In the Philippines the demand for wood and its derived products is increasing, while areas devoted to tree plantations are shrinking. Millions of quality planting materials are needed to meet the increasing demand for wood and derived products and address the decreasing land area available to production forest. Based on Philippine Forestry Statistics (2012), the country’s total log production amounts to 862,429 cubic meters (approximately 0.008 cubic meter per capita) and logs from plantation forests accounted for the 99.6% of this value. This amount, however, is remarkably low compared to the world’s average per capita production of 0.5 cubic meters (FAO 2002). If this low rate is sustained, the harvests of timber from the remaining natural forest reserves would continue. Thus, there is an urgent need to propagate trees that are fast growing and with valuable utility traits. However, forest tree plantations establishment has been restricted by lack of high quality and yield potential planting materials. In addition, classical tree improvement has been hampered by the time and areas needed to assess and select superior planting material. The choice of suitable planting stock origins is the main element of plantation success while advanced breeding strategies and efficient nursery and in vitro vegetative propagation methods can hasten the mass production of genetically improved planting stock. One industrial tree plantation species planted throughout the Philippines that requires biotechnological inputs is Falcataria moluccana, locally known as Moluccan sau (in CARAGA it is known as Falcata). The tree is a fast growing species with an annual average increment of 30m3/year. It is popular in South East Asia for pulp and paper making, veneer manufacturing, light furniture making, and other end-uses. In spite of its economic importance, very limited efforts have been devoted for improving the adaptability, including pest and disease resistance, growth, phenotypic, and wood traits of the species. Growth and yield of plantations of F. moluccana were low (60 cubic meter/ha eight years after planting), which is mostly due to poor genetic selection of seed sources and poor silvicultural practices. They have also been attacked by the gall rust pathogen, but there are trees found resistant and they are widely grown in the CARAGA region in Mindanao. The proposed program aims to contribute to the conservation and efficient production of superior quality planting materials of F. moluccana. The expected outputs of the project are 1) Selection and conservation of gall rust resistant plus trees and gall rust resistant non-plus trees of F.moluccana; and 2) Establishment of Clonal Seed Orchard and plantation from clones of gall rust resistant plus trees of F.moluccana . The primary beneficiaries of the proposed program are the local tree farmers of CARAGA region in Mindanao through increased income from the enhanced production of quality planting materials nurseries. The proposed program will also provide baseline information (e.g. genetic diversity level and structure, candidate gene(s) for gall rust resistance, and wood quality traits of gall rust resistant plus trees and gall rust resistant non-plus trees) for future tree breeding program for F. moluccana. This program will be executed and implemented by the College of Forestry and Natural Resources, University of the Philippines Los Baños in collaboration with the DENR-CARAGA and CIRAD.The results of the proposed program will be shared publicly through presentations and written scientific journals, and it will be disseminated and discussed to the local tree farmers with the coordination of the DENR. Management of the clonal orchards in the CARAGA region will be under the supervision of the DENR-CARAGA for long-term maintenance. Funding contributions from the ITTO amounting to US$ 1,075,101 and contribution in kind from the Government of the Philippines amounting to US$ 162,300 are requested in order to execute the whole program of activities.

-ii-

Page 4: ITTO · 2015. 10. 6. · international tropical timber organization . itto. project proposal . title: genetic conservation, utilization and management of gall rust resistant strains

List of abbreviation and acronyms

CFNR College of Forestry and Natural Resources

CIRAD Centre De Coopération Internationale en Recherche Agronomique Pour Le Développement (French Agricultural Research Centre for International Development)

CSO Clonal Seed Orchard

DENR Department of Environment and Natural Resources

DOST Department of Science and Technology

EA Executing Agency

FBS Forest Biological Sciences

FBP Forest Biotechnology Program

FMB Forest Management Bureau

FTFDI Federation of Tree Farmers and Developer Inc.

ITTO International Tropical Timber Organization

LGU Local Government Unit

NGO Non-government Organization

NIMBB National Institute of Molecular Biology and Biotechnology

PCAARRD Philippine Council for Agriculture, Aquatic and Natural Resources Research and Development

PGC Philippine Genome Center

UPLB University of the Philippines Los Banos

-iii-

Page 5: ITTO · 2015. 10. 6. · international tropical timber organization . itto. project proposal . title: genetic conservation, utilization and management of gall rust resistant strains

Map of Project Site

Figure 1. Map of the CARAGA Region in Mindanao, Philippines. The location of Falcataria

moluccana tree farmers ‘cooperators’ of the DENR CARAGA is indicated by red dots. CARAGA Map DENR-CARAGA

Tree farmer ‘cooperators’

-iv-

Page 6: ITTO · 2015. 10. 6. · international tropical timber organization . itto. project proposal . title: genetic conservation, utilization and management of gall rust resistant strains

PART 1. PROJECT CONTEXT 1.1 Origin The Department of Science and Technology (DOST) and Philippine Council for Agriculture, Aquatic and Natural Resources Research and Development (PCAARRD) had funded a Forest Biotechnology Program (FBP) that was implemented at the CFNR-UPLB in 1991. The second phase of the FBP on “Applications of Forest Biotechnology for the Mass Production of Genetically Superior Trees for Agroforestry, Community-Based Forestry and Industrial Forest Plantations” was funded by DOST-PCAARRD in 2000-2002. All this was supported by several back-up missions from CIRAD Projects on selection, macropropagation, and micropropagation of plus trees of Acacia mangium, Swietenia macrophylla, Pterocarpus indicus, Gmelina arborea, and Eucalyptus deglupta were conducted. Some of the rooted cuttings of plus trees selections were planted at the vicinity of the National Institute of Molecular Biology and Biotechnology (NIMBB, formerly BIOTECH) of UPLB. Procedure for the rooting of cuttings of A. mangium X A. auriculiformis putative hybrid, G. arborea, E. deglupta X E. pellita hybrid, P. indicus, and S. macrophylla had been accomplished. Protocol for micropropagation of A. mangium, using materials from mature trees, was also developed. The protocol initially developed for E. deglupta was successfully applied to tissue culture of E. deglupta X E. pellita hybrids. The implementation of the second phase of the program had also enabled the establishment of a tissue culture laboratory at the Department of Forest Biological Sciences (FBS), CFNR-UPLB, College, Laguna. The laboratory had also been equipped with other facilities such as water distilling apparatus, autoclave, and refrigerators, through the donation of the French embassy. The proposed three-year program will use the said laboratory facility. In 1997 a provenance trial of F. moluccana growing in Surigao del Sur in Mindanao was implemented, which involved 14 provenances and two land races. The provenances were evaluated at several growth parameters such as diameter at breast height, total height, canker disease incidence, bole form and volume, and exceptional individual trees. With the assistance of the DENR-CARAGA, these provenances will be surveyed and utilized for selection and collection of plus trees. 1.2 Relevance 1.2.1 Conformity with ITTO objectives Relevance to ITTA 2006 The proposed program is relevant to the following objectives of ITTA 2006: Objective (c): Contributing to sustainable development and to poverty alleviation Objective (f): Promoting and supporting research and development with a view to improving forest

management and efficiency of wood utilization, as well as increasing the capacity to conserve and enhance forest value in timber producing tropical forests

Objective (i): Promoting increased and further processing of tropical timber from sustainable sources in

producer member countries, with a view to promoting their industrialization and thereby increasing their employment opportunities

Objective (j): Promote and support research and development with focus to improve forest management

and efficiency of forest utilization as well as increase the capacity to conserve and enhance other forest values in timber producing tropical forest and the promotion of research and development in forest management.

Objective (r): Encouraging members to recognize the role of forest-dependent indigenous and local

communities in achieving sustainable forest management and develop strategies to enhance the capacity of these communities to sustainably manage tropical timber producing forest.

Relevance to ITTO Strategic Action Plan 2013-2018 This program is also relevant to the ITTO Strategic Action Plan 2013-2018 specific to Strategic Action Plan No. 4 on reduction of tropical deforestation and forest degradation. By providing sustainable source of quality timber of F. moluccana it will ease the pressure directed to harvesting of timbers from natural forests.

-1-

Page 7: ITTO · 2015. 10. 6. · international tropical timber organization . itto. project proposal . title: genetic conservation, utilization and management of gall rust resistant strains

1.2.2 Relevance to the policies of the Philippines The proposed program supports the Philippine Master Plan for Forestry Development, which aims to (a) contribute to the employment and growth of national and local economies through establishment and integration of forest-based enterprises, and (b) meet the demands for wood products through sustainable management of all production forests. The program will operate under the Department of Environment and Natural Resources (DENR) Order No. 35 issued in 1999, which permits utilization and harvesting in areas where communities have established their own plantation forests. 1.3 Target Area 1.3.1 Geographical location The CARAGA Region (Region XIII) was created through Republic Act No. 7901 on February 25, 1995. It is located in the northeastern section of the island of Mindanao in the Philippines (Figure 1). It is bounded on the north by the Bohol Sea; on the south by the provinces of Davao, Compostela Valley, and Davao Oriental of Region XI; on the west by Bukidnon and Misamis Oriental of Region X; and on the east by the Philippine Sea and the Pacific Ocean. It has a total land area of 2,141,298 ha, which is divided into Agricultural Land (523,407 ha), Temporary Crop Land (189,994 ha); Permanent Crop Land (312,152 ha); Meadows/Pasture (812 ha); Forest Land (5,498 ha); Idle Land (3,317 ha); and Other Land (9,841 ha). The region is composed of 5 provinces, 6 cities, 67 municipalities, and 1,311 barangays, the smallest unit of the local government in the Philippines (Philippine Statistics Authority). It is made up mostly of mountainous areas, flat, and rolling lands. The Diwata Mountain Ranges divide the Agusan and Surigao provinces and sub-ranges separate most of the lowlands along the Pacific Coast. Most productive agriculture area lies along the Agusan River Basin. The famous Agusan Marsh sits in the middle of Agusan del Sur. Among the lakes in the region, Lake Mainit is the widest, covering eight municipalities: Alegria, Tubod, Mainit and Sison in Surigao del Norte and Tubay, Santiago, Jabonga and Kitcharao in Agusan del Norte. Agusan del Sur is a landlocked province. Surigao del Sur is a coastal province. Both these provinces are mountainous with more than half of the provincial areas classified as forestland–76% of 896,550 hectares in Agusan del Sur and 71% of 455,216 hectares in Surigao del Sur. CARAGA has excellent tourism potentials because of its unspoiled and beautiful beaches, abundant and fresh seafood, ancient and historical landmarks, hot and cold springs, evergreen forests, and balmy weather. The region generally does not have definite dry season. Rainfall occurs throughout the year with heavy rains from November to January. Storms occur on the northern and eastern portions facing the Pacific Ocean; the rest is relatively typhoon-free. 1.3.2 Social, cultural, economic, and environmental aspects Based on the 2010 Philippines census data, the population of CARAGA is 2,429,224, with a growth rate of 1.49% from 2000. Majority of the population of the region are of Visayan origin. Some of the ethnic residents include the Manobo (35% of the population), Mamanwa, and other tribes. During the early years of the CARAGA region, the inhabitants came from the mainland Asia, followed by Malayans, Arabs, Chinese, Japanese, Spanish, and Americans. Migrants from the Visayan and Luzon provinces later settled in CARAGA. Majority of the population speaks Cebuano (about 44%) and resides in the rural areas. Other dialects are Surigaonon, Kamayo, Boholanon, Manobo, Butuanon, and Hiligaynon. Based on the current and 2000 constant prices, the Gross Regional Domestic Product (GRDP) of CARAGA are PhP109,765,000 (US$2.7 million) and PhP68,592,000 (US$1.7 million), respectively. The Gross Value Added (GVA) in Agriculture, Fishery, and Forestry based on the current and 2000 constant prices are PhP30,248,000 and PhP14,843,000, respectively. In 2011, the GRDP of CARAGA grew by 9.56%. The Agriculture, Fishery, and Forestry Sectors accounted for 21.64% of the regional economy. CARAGA contributed 1.16% to the country’s Gross Domestic Product (GDP). In 2011, the total employment in the region was 1,006,000 of which about 39% was absorbed by the agriculture sector. Of those employed in agriculture, 76% are male workers. The leading crops in CARAGA are palay, banana, and coconut. These crops contributed 41.22% of the region's agricultural output. The region was number 13 in the national production of palay, 9th for coconut, and 10th for banana. The production in agriculture, however, decreased by 9.14% in 2011.

-2-

Page 8: ITTO · 2015. 10. 6. · international tropical timber organization . itto. project proposal . title: genetic conservation, utilization and management of gall rust resistant strains

CARAGA has fertile land, favorable climate, and appropriate elevation, which are favorable for tree plantation. Thus, tree plantation has become an important economic activity in the region. Tree farming is an established livelihood in the region, covering approximately 46,000 hectares of private lands, which involves 31,000 tree farmers. CARAGA has become the major source of timber for Mindanao and the rest of the country, with potential area of about 116,000 hectares of private lands that can be developed into private tree plantations. In 2000, timber produced from private tree farms was 60% of the Philippines log production (800,000 cubic meters). Based on the recent Philippine Forestry Statistics (2012), the production of logs during the review year totaled 862,429 cubic meters, which is about 1% less than the production of the preceding year. Logs harvested from plantation forest accounted for 99.6% of the total production (858,888 cubic meters), while naturally grown logs shared only 0.4% to the total log production, dropping by 76% from the previous year as a result of moratorium on the cutting of trees from the natural and residual forests as directed under Executive Order 23. CARAGA had the biggest share in the total log production with 584,257 cubic meters or 68%. Timber traded in the region was F. moluccana. Sawlog timber of size 30 cm and bigger was sold at an average price of PhP 2,367 per cubic meter or 56.13% higher than the 2011 record. Peeler/veneer log of F. moluccana was priced at an average of PhP 1,544 and PhP 2,249 for diameter size below 30 cm and diameter size 30 cm and bigger, respectively. F. moluccana has a ready market in CARAGA and its low wood density favors efficient transport and loading. It also reaches economic maturity in a shorter period, thus, it generates higher profit than other tree crops. In the domestic market, logs F. moluccana , with small end diameter of 12 cu.m. and above, can be sold at the mill door from PhP1200/cu.m. to PhP3200/cu.m. In the international market, the species is sold as sawnwood. The region is also rich in natural resources, with large tracts of available land for development. It has extensive water resources, and rich mineral deposits such as iron, gold, silver, nickel, chromite, manganese, and copper. The proximity of CARAGA to other growth areas such as the Cagayan-Iligan corridor and the Davao Gulf Economic Zone gives the region the economic advantages. The entire region is connected by roads from and to the major commercial, trading, and processing centers of Cagayan de Oro and Davao. Regional center with modern facilities is also being developed. Secondary seaports and airports are also present in CARAGA. 1.4 Expected outcomes at project completion This proposed research and development program will help small scale tree farmers in the Philippines, particularly those in the CARAGA region, to increase their income from tree farming as a result of the technology and information that made the supply of high quality planting materials for plantation establishment sufficient. This will later provide avenue for creating more local employment and opportunities for establishing new small scale industries and reinvigorating existing ones. When these are realized, it will invite more investment and more financial and logical support devoted for tree farming in the region and throughout the country. Ultimately, the project intervention will contribute to the betterment of the socio-economic condition of tree farmers in CARAGA region.

-3-

Page 9: ITTO · 2015. 10. 6. · international tropical timber organization . itto. project proposal . title: genetic conservation, utilization and management of gall rust resistant strains

PART 2. PROJECT RATIONALE AND OBJECTIVES 2.1 Rationale 2.1.1 Institutional Set-up and Organizational Issues. The UPLB-CFNR will be the Executing Agency of the program since most of the project activities will be done in the University of the Philippines Los Baños College of Forestry and Natural Resources. UPLB-CFNR will implement all projects in active collaboration with the two collaborating agencies (i) DENR-CARAGA and (ii) CIRAD. In the implementation of project activities, UPLB-CFNR will share the responsibilities with these agencies, and will comply with national and ITTO rules and regulation, will also work indirectly with the (iii) local tree based farmers, (iv) LGUs, and (iiv) NGOs and national research institution. local LGUs and other relevant institutions. The overall project findings and outputs are disseminated and coordinated by UPLB i) DENR-CARAGA is the implementer of policies related to forest resources utilization and has

direct control of forest related activities in the area and been involved in setting national strategy for tree breeding program. They will contribute to the identification of the local tree based-farmers in the area to take part in the project since it has identified established tree farmers’ federation since 2002.Their involvement will range from assistance and coordination in the provenance survey, sampling, and preparation of sites for nursery and clonal orchards and their maintenance. UPLB-CFNR and DENR-CARAGA have a good and long standing working relationship in the management of Mindanao’s forest particularly in Caraga Region. They have also collaborated in execution of many projects including international donor funded projects like ITTO, ACB, CIRAD, CSIRO and AKECUP. This long standing relation between the executing agency and UPLB-CFNR will contribute to the effective implementation of this project

ii) CIRAD has extensive experience in selection, breeding and propagation of trees as well as on

marker-assisted selection/analyses. Hence, they will provide technical expertise in terms of training in the fields of genetics and biotechnology. They will be involved in the refinement of protocols for macro- and micropropagation, and mass production of clones and in the implementation of the study on F. moluccana-rhizobial and mycorrhizal association specifically on the following activities; Characterization and identification of effective mycorrhiza and rhizobium-root associations; Collection, isolation, and screening of mycorrhiza and rhizobium strains; Inoculation of clones with mycorrhizal fungi and rhizobial bacteria; Assessment of its effect in promoting survival and growth of clones; and Formulation of biocontrol to combat gall rust. Their recommendations and significant inputs are indispensable in the success of this project.

2.1.2 Stakeholder analyses Stakeholder identification and consultation The DENR-CARAGA and the UPLB-CFNR are the two relevant institutions to be primarily involved in this program. The involvement of the DENR-CARAGA will range from assistance and coordination in the provenance survey, sampling, and preparation of sites for nursery and clonal seed orchards in the CARAGA region and their maintenance. The UPLB-CFNR, as the lead proponent of this program, will be involved in the coordination and implementation of all activities of the program. CIRAD will provide technical expertise in genetics and biotechnology specifically in the implementation of the study on F. moluccana-rhizobial and mycorrhizal association and biocontrol formulation and development. However, the primary stakeholders/beneficiaries of this project are the tree-based farmers of CARAGA in Mindanao. The CARAGA Federation of Tree Farmers and Developers, Inc. (FTFDI) In CARAGA, there is an association of small tree farmers, i.e the CARAGA Federation of Tree Farmers and Developers, Inc., which ‘initiates tree farming in private lands, leads farmers in sustainable livelihood projects in pursuit of poverty reduction and economic development, and serves as the unifying organization for pro-active policy towards a favorable investment climate”. The association envisions empowering farmers to engage in sustainable livelihood project through tree plantation. The creation of the federation was initiated by the DENR to unite all private tree farmers in the region. Along with the establishment of the federation, a resolution, which called for a pilot deregulation of harvesting and transport of planted timber products, was signed upon by the

-4-

Page 10: ITTO · 2015. 10. 6. · international tropical timber organization . itto. project proposal . title: genetic conservation, utilization and management of gall rust resistant strains

federation, DENR, and Philippine Wood Producers Association. As an answer to the call of deregulation, a Regional Memorandum Order 02-014 was issued by the DENR CARAGA mandating the federation to undertake the function of documenting the planted timber harvested from private and Alienable & Disposable (A&D) lands as an interim procedure. Members of the federation are entitled to free 500 seedlings and 50% discount on additional seedling requirements; free seed requirements; access to Zero Interest Loan; provision of technical assistance; financial assistance; assistance during trucking and towing; and fast track of documentation of the planted timber harvested from private and A & D lands as an interim procedure. Since its establishment, it has generated direct and indirect employment (116 men and women, of which 31 are registered foresters, 11 are BSF graduates and 74 members of other professions). It has benefitted about 1,139 farmers from augmented income, with an increase of 28% of their net income. The federation has already established 27 nurseries, producing 314,000 seedlings per month, and three model farms (Mitchao 2004). The DENR-CARAGA and FTFDI, particularly farmers engaged in Falcata production, has a long strong standing collaboration since 2003 when the federation was created. The federation sprouted in response to the adverse market and policy conditions governing the small-scale tree farming industry. In the conceptualization of this proposal, there was no actual participatory stakeholder analysis conducted in the target area; however, the DENR-CARAGA, which has direct involvement with the tree farmers in CARAGA as the primary beneficiaries identified by this program, was consulted when the proposal was conceptualized. Their characteristics, problems, needs, as well as interests related to the proposed program were identified based on secondary data such as reports of DENR, FTFDI, and other secondary sources that evaluated tree farming in CARAGA. Consequently, the information gathered was used to generate the problem and objective trees. The level of commitment of the stakeholders, including tree-based farmers such as the FTFDI and a number of Falcata tree incorporators of the DENR-CARAGA to the proposed program is expected to be strong. However, there are a number of challenges and constraints faced by small-scale tree farming that were identified by the federation; 1) political intervention; 2) lack of initial database as to the extent, age and species of plantation; 3) lack of technical support; 4) absence of proper market linkage for the products; 5) lack of quality–driven products from seed to wood management; and 6) minimal community and capability building. Through this project, many of their challenges will be positively addressed. The wood industries that will buy timber products from the communities and the non-government organizations and the local government units which will assist tree farmers are identified as the tertiary stakeholders. The wood industries, Local Government Units (LGUs), and Non-Government Organizations (NGOs) will have an indirect role in the implementation and success of the program. The wood industries as buyer of timber products of local tree based farmers in CARAGA will provide long-term partnership with the tree farmers. The LGUs being implementer of local policies and regulation related to business opportunities and requirements have strong collaboration with the DENR-CARAGA. They are also buyer and user of selected seeds of F. moluccana from Mindanao Tree Seed Center (MTSC), one of the few sources of quality seeds. LGUs are expected to give support for the community by providing a business-friendly regulations for small holder farmers to set up business venture. NGOs which are capable of close collaboration with tree farmers are expected also to raise awareness of community about the project. The Philippine Genome Center having experts in genomics and bioinformatics will provide technical assistance in screening candidate genes resistance for gall rust and also help in the analyses of the genomics data. All of them have limited or no direct control over the implementation of the project findings.

-5-

Page 11: ITTO · 2015. 10. 6. · international tropical timber organization . itto. project proposal . title: genetic conservation, utilization and management of gall rust resistant strains

Stakeholders Group

Characteristics Problems, needs, interest

Potentials Involvement in the project

Primary stakeholder

Local tree-based farmers/small-scale tree-based farmers

Users of forest resources; producers of timbers

Lack of knowledge and skills in production of quality planting materials

Minimal support for their activities from the government due to absence of clear cut policy on tree farming in private lands

Lack of initial database as to the extent, age and species of plantation; Lack of technical support; Absence of financial windows for soft loans either from the government or private lending institution; Absence of proper market linkage for the products; Not properly organize in cooperation with other stakeholders; Lack of quality–driven products from seed to wood management; Absence of investment incentives and/or investment attraction; Minimal community and capability building

Capable of enhanced production of timber

Involvement in field plantation establishment; participation in capacity enhancement program

-6-

Page 12: ITTO · 2015. 10. 6. · international tropical timber organization . itto. project proposal . title: genetic conservation, utilization and management of gall rust resistant strains

Stakeholders Group

Characteristics Problems, needs, interest

Potentials Involvement in the project

Secondary stakeholder

DENR-CARAGA

Implementer of policies related to forest resources utilization; has direct control of forest related activities; involve in setting national strategy for tree breeding program

Limited technical people to execute some of the methods to be employed by the program

Capable of influencing tree-based farmers and their association, if any, to utilize planting materials derived from this program; facilitate implementation of the project findings

Coordination of activities in the study site (provenance survey, selection of plus trees, collection of samples, establishment of nursery and clonal orchard in the CARAGA region

UPLB-CFNR Educator; involved in research and development

Has limited control over implementation of the project findings beyond the duration of this program (e.g. granting permits for plantation establishment)

Capable of influencing policy makers to set national strategy for tree breeding through provision of scientific information

Coordination and implementation of all activities of the program

CIRAD Involved in research and development on tree improvement and vegetative propagation techniques as well as on symbiosis

Has limited control over implementation of the project findings

Extensive experience in selection, breeding, and propagation of trees as well as on marker-assisted selection/analyses

Involvement is limited to specific activities

Tertiary Stakeholders

Market

- Agents

- Traders

- Financiers

Buyer of the timber

- finds wood sources and earns commission per cubic meter

- finds financiers and processes transport documents

Has tendency to monopolize market price of timber

Traders could dictate the purchasing cost by bulk/whole sale (per area)

May provide long-term partnership with the farmers

End-user

-7-

Page 13: ITTO · 2015. 10. 6. · international tropical timber organization . itto. project proposal . title: genetic conservation, utilization and management of gall rust resistant strains

Stakeholders Group

Characteristics Problems, needs, interest

Potentials Involvement in the project

- Wood processing plants

- negotiates with the wood processing plant for purchase orders and provides operational capital

- buys and processes tree species

LGUs Implementer of local policies and regulation related to business opportunities and requirements; some LGUs are buyers of selected seeds of F.moluccana from Mindanao Tree Seed Center (MTSC) for their greening program

Some bureaucratic procedures prolong issuance of business activity permits, and other documents needed by farmers

Strong collaboration with the DENR-CARAGA

Support for the community by providing a business-friendly regulations for small holder farmers to set up business venture

NGOs Social support system

Has limited control over decision-making

Capable of close collaboration with tree farmers

Awareness raising for the community

2.1.3 Problem analysis The Philippines needs sufficient supply of superior quality timber to meet the increasing demand for wood and wood derived products but without placing too much pressure on harvest from natural forests, which are dramatically shrinking. This prompted the need to propagate fast-growing and high-yielding tree plantation species, resorting to advanced breeding strategies and efficient nursery and in vitro vegetative propagation methods (e.g. tissue culture). F. moluccana is a popular industrial tree plantation species in the Philippines. The tree has various uses for pulp and paper making, veneer manufacturing, light furniture making, and other end-uses. However, growth and yield of plantations of F. moluccana were low, mostly due to poor selection of planting materials (mostly seeds) and seed sources and poor silvicultural practices. Many plantations had seriously been affected by the gall-rust pathogen Uromycladium tepperianum. To date, management of gall rust disease has been mostly silvicultural. For example, since occurrence of the disease in seedlings is observed in higher elevations, management of disease was done by planting the seedlings at elevations lower than 250 m. The now defunct Paper Industries and Paper Corporation (PICOP) had also an integrated approach in the disease management from seed to plantation management. In recent years there has been a growing interest in the use of biological control to manage diseases in many crops, including forest trees. Krisnawati et al (2011) and Eusebio (1998) reported microorganisms that have the potential to be used to prevent the disease such as Penicillium italicum, Acremonium recifei, and Tuberculina species. However, there is still lack of information about the protocol management of the disease through the application of these biological controls. As for U. tepperianum there has been no other microorganisms reported yet to

-8-

Page 14: ITTO · 2015. 10. 6. · international tropical timber organization . itto. project proposal . title: genetic conservation, utilization and management of gall rust resistant strains

have antagonistic property to this fungal pathogen. In CARAGA, trees resistant to gall rust disease have been found widely growing in the region. There are several factors that affect the success of any planting activity. This includes low survival of the outplanted materials, which may be due to low quality of seeds and inferior planting stocks, poor site-species selection, absence of species trial, lack of documentation of seed origin, and absence of seed quality testing and technology (Lapis, 2006; Tolentino 2006). One way to produce millions of superior quality planting materials is through the intervention of biotechnology. Tissue culture offers a reasonable cost and fast method of producing millions of high quality planting materials. One tropical tree that has been mass produced through tissue culture is Tectona grandis (or Teak). Millions of rooted cuttings and microcuttings of clonally propagated teak trees have been produced and planted by private companies worldwide, since the mid-1990s. Owing to the superiority of such planting material compared to seedlings, teak clonal plantations are rapidly expanding due to the high demand of private investors with the aim to maximize their return on investments in the shortest time (Monteuuis et al., 2004; Goh and Monteuuis 2005). This will help to ensure that the excellent properties of teak continue to be enjoyed, regardless of the declining availability of natural stocks. Millions of cloned teak plantlets have been produced in different laboratories using tissue culture. The rooted cuttings or microcuttings have performed satisfactorily in the field, remaining true-to-type. In the Philippines, commercially available protocol on tissue culture of genotypically superior trees has not been available yet. The only tree species that can be mass produced through tissue culture of explants from mature trees is Eucalyptus deglupta, an endemic eucalyptus species in the Philippines and also an important ITP in CARAGA (Calinawan et al.). The rest of the tissue culture protocols of trees reported to date had used parts from in vitro germinated seeds. But since trees are cross-pollinated, these tissue culture protocols will not produce the desired phenotypically superior trees. In addition, due to the lack of efficient policies and national strategy devoted on forest crop improvement and breeding, there are a number of initiatives that failed to address the low yield of industrial tree plantations as a result of poor quality planting materials used. The absence of a national strategy has led to limited available funding for national research programs that are expected to give information for selection of parent materials for tree breeding programs.. Likewise, with the absence of national strategy on forest crop improvement and breeding, the support for the local and small scale tree farmers toward capacity enhancement (e.g. skills in selection and propagation of quality planting materials and proper plantation establishment) was also limited. Problems encountered within the context of SFM In the Philippines small-scale tree farming on private lands was an offshoot of large-scale logging operations since 1950s. It drew migrants who sought employment in logging companies and also triggered the interest of people in logging as their primary source of income next to farming. However, when the national government issued log bans in the 1990s, many workers in logging concessions established their own tree farms. The Philippine national government selected the development of agri-business as one of its key priorities in its 10-point development agenda. Tree farming on private lands contributes to internal revenue, creates local employment, and boosts the local economy, and contributes greatly to domestic wood supply. Small-scale tree farming on private lands, however, is still considered a forest activity in which strict regulatory measures apply. At present, small-scale tree farming is suffering major setbacks in terms of poor policy support (e.g. seed and wood certification) and inconsistent policy implementation. Enabling mechanisms such as financial support and technical assistance for tree farm development are also lacking. Currently, the strength of the tree farming industry in CARAGA lies with the farmers who plant fast-growing species on their own lands with limited or no assistance from the government. In the 1970s to 1980s, interest on planting trees increased because of the large market for wooden electric poles, the declining relative prices of corn and rice, and the massive reforestation program on public forestlands. In the late 1990s, however, the cement industry came up with a more durable substitute for wooden electric poles, bringing down the price of logs from fast-growing species. Thus in response to the adverse market and policy conditions governing the small-scale tree farming industry, the FTFDI was formed. Marketing the wood from private tree farms is a complex process. There are several intermediate stakeholders that the tree farmer must deal with before finally getting his logs to the end market, including, traders, financiers, and road checkpoints. The tree farmer’s income is partly determined by the distance of his or her tree farm from the highway. Wood can be sold to the trader by

-9-

Page 15: ITTO · 2015. 10. 6. · international tropical timber organization . itto. project proposal . title: genetic conservation, utilization and management of gall rust resistant strains

bulk/wholesale (per area), or by wood volume (per cubic meter). If sold by area, the price would be determined by the size of the farm as well as the number and size of the hills, and the trader shoulders the cutting and hauling costs. If sold by volume, the price would depend on the volume of logs harvested excluding rejected logs and the tree farmer shoulders the felling, skidding (by carabao) and hauling costs. Farmers are seeking ways to increase diameter growth and correctly grade logs to take advantage of higher prices for large diameter logs. An average truckload of falcata carries around 30-35 cubic meters consisting of around 200 pieces of large diameter logs and more than 500 pieces of the smaller logs. Average cost of production is around PhP960/cu.m (Rp168,000) including felling and bucking, yarding, loading, transport, local and national taxes, and formal and informal checkpoint tariffs. Most small-scale tree farmers market their wood in Butuan City or Davao City. Some farmers in Agusan del Sur are able to sell to the 6 small- and medium-scale sawmills along the route to Butuan. Surigao del Sur has commercial-scale wood processing plant attached to a timber concessionaire. The plant produces plywood and sources raw materials only from its concession. Surigao del Sur also has areas with Community based Forest Management Agreements managed by peoples’ organizations that have Falcata and Mahogany plantations and process timber into wood crafts, souvenir items, and furniture. In order to address the above problems we are proposing a three-year research and development program that aims to address the insufficient supply of quality planting materials and low yield of existing plantations of F. moluccana . The program is envisioned to ultimately help the local tree farmers of CARAGA region in Mindanao by contributing to the improvement of their socio-economic condition through increased income from enhanced yield of plantations. The program would also benefit the research and academic sectors by establishing baseline information for developing tree breeding strategy for F. moluccana and consequently would prompt the Philippine government to legislate policies directed towards development of a national strategy on forest crop improvement and tree breeding.

-10-

Page 16: ITTO · 2015. 10. 6. · international tropical timber organization . itto. project proposal . title: genetic conservation, utilization and management of gall rust resistant strains

Figure 2 Problem tree

Lack of information for economically traits to be used for producing and planting superior planting material

Lack of efficientnational strategies for boosting tree plantation productivity

Low yield of existing industrial tree plantations to meet the increasing demands for wood and wood-based products

Insuf f icient availability of superior quality planting materials of industrial tree plantation species, with special mention for F. moluccana

Lack of skills of local tree-based farmers on propagation of superior quality planting materials

Lack of knowledge of local tree-based farmers on selecting for superior quality planting materials

Absence of knowledge on genetic variation of the species

Low income of tree-based farmers

Lack of advanced techniques, methods, and protocols to combat gall rust disease in F. moluccana

Diminished interest to invest in industrial tree plantation establishment

High price of quality seeds

Very limited source of quality/certified seeds; seeds are sold conventionally in inappropriate nurseries (e.g. highways)

Lack of information education campaign on the availability of quality seeds

Unsustainable seed production of mother trees/ Seed production areas are not sustainably managed

Absence of policy on seed certification

Lack of training and capacity building on seed technology and planting stock production

Lack of initial database as to the extent, age and species of plantation

Lack of quality– driven products from seed to wood management

Lack of research on establishment of science about gall rust in F. moluccana and its possible biocontrol

Lack of technology on clonal production of the gall-rust resistant F. moluccana

-11-

Page 17: ITTO · 2015. 10. 6. · international tropical timber organization . itto. project proposal . title: genetic conservation, utilization and management of gall rust resistant strains

2.1.4 Logical framework matrix

PROJECT ELEMENTS INDICATORS MEANS OF VERIFICATION

IMPORTANT ASSUMPTION

Development Objective To contribute to the improvement of the socio-economic condition of the tree farmers in CARAGA region by increasing their income from the high yield of F. moluccana plantations established from superior quality planting materials

Number of tree farmers engaged in establishment of plantations of improved F.moluccana increased by at least 50% by 2025 Annual yield of plantation of F. moluccana (30 cu.m/yr) increased by at least 50% by 2025 Income and employment opportunities of communities from tree plantations and related industries or enterprises increased by 50% in 2025

Community livelihood profile Community visits Membership record to the CARAGA Tree Farmers and Developer Association

Participation of target communities Concerned government and local institutions are willing to support the community by enhancing the farmers’ capacity to establish and manage plantations, to set up wood-based business venture, or to improve entrepreneurial skills

Specific Objectives To conserve and mass propagate gall rust-resistant trees plus trees of F. moluccana to increase the supply of superior quality planting materials and increase the yield of plantations

Supply of superior quality planting materials increased by at least 50% in 2020 Ex situ (Clonal Seed Orchards) conservation sites established by the end of this program

Number of plants produced

Number of clonal seed orchards field established

Such plus and gall resistant F.moluccana trees are still exisiting

Output 1 Gall rust resistant plus trees and gall rust resistant non-plus trees of F. moluccana selected, characterized and conserved

By the end of the 3rd quarter of the 1st year of the program, at least 100 gall rust resistant plus trees and 100 gall rust resistant non-plus trees were surveyed and selected, and propagules for mass propagation through cuttings and tissue culture were collected from the gall rust resistant plus trees By the end of the first year of the program, wood properties of the plus trees and gall rust-resistant trees were determined By the end of the 3rd year of the program, levels of genetic diversity and structure of at least 16 stands and/or plantations (at least 24

Progress reports Record of genotypic profiles of individuals used in the molecular analysis Published gene annotations for gall rust resistance

Coordination with the DENR-CARAGA and concerned local government units and tree plantation owners are properly established Issuance of permit(s) or related documents for collection of biological resources is expedited Complete facilities for molecular analysis and wood quality determination is available Enhanced procurement of laboratory chemicals

-12-

Page 18: ITTO · 2015. 10. 6. · international tropical timber organization . itto. project proposal . title: genetic conservation, utilization and management of gall rust resistant strains

individuals per plantation), representing the 14 provenances and 2 land races of F.moluccana used in the provenance trial in CARAGA, including selected gall rust resistant trees, were evaluated By the end of the 3rd year of the program, at least one candidate gene for resistance to gall rust was identified

Compatibility of mycorrhiza and rhizobia with F. mollucana is determined

Output 2 Clonal Seed Orchards of and clonal plantation of F.moluccana established

Superior quality F.moluccana planting materials were mass produced through macropropagation/cuttings (at least 1000 by the 2nd year and at least doubled by the 3rd year) and tissue culture (at least 1000 by the end 2nd year and at least doubled by the 3rd year) By the end of the 3rd year, the planting materials produced through both cuttings and tissue culture were acclimatized and evaluated for growth and survival with rhizobium and mychorrizal inoculants under nursery condition By the end of the 2nd year at least one biocontrol for gall rust was screened and formulated by the end of the 3rd year By the end of the 2nd quarter of 3rd year of the program, clonal orchard containing at least 100 plus trees (at least 3 ramets per one ortet/mother tree) was established in CARAGA and duplicated in UPLB

Progress reports Observation and monitoring record Published/reported protocol for gall rust bioncontrol Biocontrol formula

Land for clonal orchard establishment for long term use is provided by the DENR-CARAGA and UPLB Processing and procurement of laboratory chemicals and nursery equipment are expedited Potential organism for biocontrol formulation is available

-13-

Page 19: ITTO · 2015. 10. 6. · international tropical timber organization . itto. project proposal . title: genetic conservation, utilization and management of gall rust resistant strains

2.2 Objectives Figure 3 Objectives tree

Information for economically traits for producing and planting superior planting material is available

Efficient national strategies for boosting tree plantation productivity implemented

High yield of existing industrial tree plantations and met the increasing demands for wood and wood-based products

Supply of superior quality planting materials of industrial tree plantation species, with special mention for F. moluccana is sufficient

Local tree-based farmers are skilled on propagation of superior quality planting materials

Local tree-based farmers have knowledge on selecting for superior quality planting materials

Genetic structure of the species is assessed and made available

Income of tree-based farmers increased

Advanced techniques, methods, and protocols to combat gall rust disease in F. moluccana are made available

Interest to invest in industrial tree plantation establishment increased

Quality seeds are reasonably priced

Source of quality/certified seeds are made available; seeds are sold in appropriate nurseries

Information on the availability of quality seeds disseminated

Seed production of mother trees/ Seed production areas are sustainably managed

Policy on seed certification crafted

Local tree-based farmer are trained on seed technology and planting stock production

Initial database as to the extent, age and species of plantation existed

Quality– driven products from seed to wood management existed

Science about gall rust in F. moluccana and its biocontrol is available

Technology on clonal production of the gall-rust resistant F. moluccana is available

-14-

Page 20: ITTO · 2015. 10. 6. · international tropical timber organization . itto. project proposal . title: genetic conservation, utilization and management of gall rust resistant strains

2.2.1 Development objectives and impact indicators To contribute to the improvement of the socio-economic condition of the tree farmers in CARAGA region by increasing their income from the high yield of F. moluccana plantations established from superior quality planting materials The impacts of the development objective are:

• Number of tree farmers engaged in establishment of plantations of improved F.moluccana increased by at least 50% by 2025

• Annual yield of plantation of F. moluccana (30 cu.m/yr) increased by at least 50% by 2025 • Income and employment opportunities of communities from tree plantations and related

industries or enterprises increased by 50% in 2025 2.2.2 Specific objectives and outcomes indicators To characterize, conserve, and mass propagate gall rust resistant plus trees of F. moluccana in order to increase the supply of superior quality planting materials, thereby increasing yield of plantations The impacts of the specific objective are:

• Supply of superior quality planting materials increased by at least 50% in 2020 • Ex situ (i.e. Clonal Seed Orchards) conservation sites established by the end of this program

-15-

Page 21: ITTO · 2015. 10. 6. · international tropical timber organization . itto. project proposal . title: genetic conservation, utilization and management of gall rust resistant strains

PART 3. DESCRIPTION OF PROJECT INTERVENTION 3.1 Outputs and activities 3.1.1 Outputs Output 1 Gall rust resistant plus trees and gall rust resistant non-plus trees of F. moluccana selected, characterized, and conserved Outcome indicators Genetic structure of gall rust resistant plus trees and gall rust resistant non-plus trees ascertained Candidate gene(s) for resistance screened and identified Wood quality traits of gall rust resistant plus trees and gall rust resistant non-plus trees determined Activities

Survey of provenance, selection and collection of propagules and leaf samples from gall rust resistant

plus trees and gall rust non-plus trees Genetic characterization of gall rust resistant trees (plus trees and non-plus trees) Screening of candidate genes for gall rust resistance in F. moluccana Determination of wood quality traits of gall rust resistant trees (plus trees and non-plus trees) Output 2 Clonal seed orchard and plantation of F. moluccana established Outcome indicators Nursery and clonal orchards of F. moluccana established in CARAGA and duplicated in UPLB-CFNR

provided long-term benefits for further research opportunitiesClonal plantation (pilot) established in selected tree farmer area in CARAGA

Activities

Refinement of protocols for macro-and micropropagation and mass production of clones Characterization and identification of effective mycorrhiza and rhizobium-root associations Inoculation of clones with mycorrhizal fungi and rhizobial bacteria, and assessment of its effect in promoting survival and growth of clones Formulation of biocontrol protocol to combat gall rust Development of appropriate nursery techniques and clonal seed orchard establishment Clonal plantation established in tree farmer area

3.2. Implementation approaches and methods The proposed project requires mutual cooperation and partnership among DENR, UPLB, and CIRAD. Survey, selection, and collection of plant materials The first set of activities are selection and collection of plant materials from F. moluccana trees, which include gall rust resistant plus trees and trees that are gall rust resistant but not necessarily plus trees. Selection of plus trees will include growth and morphological traits, including stem straightness, presence or absence of epicormic branches, branch angles, among others. Wood quality such as physical and structural features will also be considered in plus tree selection. Molecular characterization In the recent years, forest tree improvement for economic traits, including health condition, has used genomic-based breeding. But because of the complexity of traits, together with the long generation, in forest trees, application of such method begin by first dissecting the complex traits in trees to

-16-

Page 22: ITTO · 2015. 10. 6. · international tropical timber organization . itto. project proposal . title: genetic conservation, utilization and management of gall rust resistant strains

their individual gene components (Neale, in press). To date, there are two major approaches for genetic dissections of complex and quantitative traits, namely; genome-wide scanning (GWS) and candidate gene approach. GWS is usually done without any presumptions on the importance of specific functional features of the traits being studied, but it is expensive and resource intensive. It only locates the chromosomal regions of quantitative trait loci (QTLs), with the aid of DNA markers under family-based or population-based experimental designs that usually embed a large number of candidate genes. On one hand, the candidate gene approach as an alternative method has been proven extremely powerful to study the genetic architecture of complex traits in many organisms, including forest trees, which is far more effective and economical for direct gene discovery (reviewed Zhu and Zhao 2007). Although the practicability of candidate gene approach is said to be limited by its reliance on existing knowledge about the known or presumed biology of the phenotype being studied, which unfortunately the detailed molecular anatomy of most biological traits particularly in forest trees remain unknown, significant progress in the identification of candidate genes involved in genetic traits has been achieved in the recent years (reviewed in Zhu and Zhao 2007). The rationale of candidate gene approach states that a major component of quantitative genetic variation of phenotype under investigation is caused by functional mutation of putative gene. Candidate genes are generally the genes with known biological function directly or indirectly regulating the developmental processes of the investigated traits, which could be confirmed by evaluating the effects of the causative gene variants in an association analysis. This approach has been ubiquitously applied in gene-disease research, genetic association studies, biomarker and drug target selection in many organisms (Tabor et al 2002). Several studies have already been conducted in the recent years to understand resistance to gall rust. Genetic variability on gall rust resistance has already been determined among different seed sources of F. moluccana in field trials. In Malaysia, seed sources of the species were tested against Uromycladium tepperianum. Wamena, a seed source from Papua, was the best to resist gall rust among six seed sources from Asia and the Pacific (Rahayu et al, 2008). The Wamena seed source was also found to be resistant to the disease among 80 open pollinated families (Baskorowati et al, 2012). Strong positive genetic correlation between diameter and disease and strong negative correlations between stem form and disease were found in this same trial of open pollinated families. A DNA analysis showed a low diversity values among 44 genotypes of the host species, F. moluccana, from 11 seed sources (Rahayu, 2009). This implies a narrow range for selection of resistance genes against U. tepperianum. Uromycladium species, its life cycle, development and genetic relationships, were also further studied. To understand the infection pathway of the gall rust pathogen to leguminous species, a laboratory trial was conducted using F. moluccana leaves and A. mangium phyllodes. Unlike in the inoculation in F. moluccana leaves where the number of germinated and penetrated teliospores was directly proportional to the hours of inoculation, no penetration of teliospores was observed in A. mangium with increase in time despite increase in the number of germinated teliospores (Widyastuti and Surya, 2013). However, morphology and phylogenetic analysis provided evidence the possibility of new or different Uromycladium species that may imply a wider range of pathogenic genes that may affect the narrow genetic variability of P. falcataria and other host species. A different species of Uromycladium, U. falcatarium sp. nov., in a DNA study was found to cause gall rust disease in Paraserianthes falcataria (syn F. moluccana) (Doungsa-ard et al. 2015). Having pool of experts in the fields of genetics, molecular biology, and biotechnology, the proponents aim to take a big leap toward tree improvement by evaluating the genetic structure of F. moluccana and screening for candidate genes for resistance against gall rust, being the major disease that have widely infected the species in the Philippines. There will be two subcomponents of the molecular assay. First, the genetic characterization of gall rust resistant trees plus trees and non-plus trees will be assessed using molecular markers. Second, for screening of gall rust resistance gene(s), published protocols related studies will be consulted for optimization of protocol for this project. Wood quality determination For a non-destructive evaluation of the wood quality, wood samples will be taken at diameter breast height (dbh) using an increment borer. From these samples, oven-dried density profile across the radius of the stem from the pith to the peripheral region would be determined using the gravimetric method. Oven-dried density is a good quality indicator because it represents the total cell wall substance present within the sample and this is directly related to the mechanical properties of the material. In addition, shrinkage behavior would be evaluated as well as the fiber biometrics

-17-

Page 23: ITTO · 2015. 10. 6. · international tropical timber organization . itto. project proposal . title: genetic conservation, utilization and management of gall rust resistant strains

particularly cell wall thickness and fiber length. Upon production of clones, the wood qualities of the clone would be compared to the characteristics of the mother trees. Macropropagation and Micropropagation For macropropagation, nodal and shoot tip cuttings from gall rust resistant plus trees will be surface sterilized and soaked in different combinations of vitamins and auxins prior to planting in potting media. The set-up will be kept under a misting system. Data on date of root of initiation, number of macro-roots, and length of macro-roots will be obtained after one month. For the micropropagation, shoot apical meristem will be collected from gall rust resistant plus trees. The methods described for teak (Monteuuis 2000) and A. mangium (Bon et al.) will be adopted by the proposed project. Mychorrhizal/Rhizobium-F. moluccana association Nursery management protocol will be optimized to include studies on associated Rhizobium and mychorrizal fungi. Screening of Rhizobium and mycorrhizal strains will be done using known laboratory fungal and bacterial cultures and mycorrhizal samples from randomly selected experimental soils from CARAGA region. These species will be assessed in terms of root colonization and their effectiveness in enhancing the growth of nursery-grown clones. In vitro bio-assay with potential bio-control agents will be conducted to screen effective fungal organisms in inhibiting development of Uromycladium tepperianum, a pathogen-causing gall rust, in artificial culture medium. The in vivo testing will be done in both clones in the greenhouse and mature trees in the field. Nursery Management The rooted cuttings and tissue culture-raised plantlets will be acclimatized under nursery conditions. Inoculation with the identified associated mycorrhiza and Rhizobium will be done. Growth performance like height, diameter, and percent survival will be monitored monthly. Fine root samples will be collected at two months after acclimatization to determine colonization of beneficial microorganism. Clonal Seed Orchard (CSO) Establishment The clonal orchard is envisioned to conserve the superior parental breeding populations and at the sametime serve as source of superior seeds for plantations. A minimum of 1 hectare of land requested from the DENR-CARAGA and UPLB-CFNR will be used for the CSO establishment. At least three ramets of each ortet (‘mother’ tree) in three replications will be used to establish the CSO of F. moluccana.Plantation establishment A two-hectare clonal plantation will be established in CARAGA through a representative tree farmer identified by DENR. The plantation will showcase the different clones from plus trees. Site reconnaissance will be done prior to planting to evaluate planting design, and scheduling of weeding and maintenance activities based on site conditions. Outplanting will follow after site preparations, such as brushing, clearing, and lay-out, are done. Regular maintenance such as watering and weeding will also be conducted. The parameters that will be monitored quarterly will be height, diameter, and percent survival. Other information like biomass, root-shoot ratio and colonization of beneficial microorganism will also be assessed.

-18-

Page 24: ITTO · 2015. 10. 6. · international tropical timber organization . itto. project proposal . title: genetic conservation, utilization and management of gall rust resistant strains

3.3 Work Plan

Output/ Activity Responsible Party Year Schedule (In Months)

3 6 9 12 15 18 21 24 27 30 33 36

Output 1 Gall rust resistant plus trees and gall rust resistant non-plus trees of F. moluccana selected, characterized, and conserved

Activity 1.1 Survey of provenance, selection and collection of propagules and leaf samples of plus trees and gall rust resistant trees

DENR-CARAGA; UPLB-CFNR; CIRAD

Activity 1.2. Genetic characterization of gall rust resistant trees (plus trees and non-plus trees)

UPLB-CFNR

Activity 1.3 Screening of candidate genes for gall rust resistance in F. moluccana

UPLB-CFNR

Activity 1.4. Determination of wood quality traits (Density, Fiber biometry, Microfibril, etc.) of plus trees and gall rust resistant trees plus trees

UPLB-CFNR

Output 2 Clonal seed orchard and clonal plantation of F. moluccana established

Activity 2.1 Refinement of protocols for macro- and micropropagation, and mass production of clones

CIRAD; UPLB

Activity 2.2. Activity 2.2.1 Characterization and identification of effective mycorrhiza and rhizobium-root associations Activity 2.2.2 Inoculation of clones with mycorrhizal fungi and rhizobial bacteria, and assessment of its effect in promoting survival and growth of clones

CIRAD; UPLB

Activity 2.3. Formulation of biocontrol protocol to combat gall rust

CIRAD; UPLB

Activity 2.4. Development of appropriate nursery techniques and clonal seed orchard establishment

DENR-CARAGA; UPLB-CFNR

Activity 2.5 Clonal plantation established in tree farmer area

-19-

Page 25: ITTO · 2015. 10. 6. · international tropical timber organization . itto. project proposal . title: genetic conservation, utilization and management of gall rust resistant strains

3.4.1 Master budget by activity (in US$)

Outputs and Activities Description

Budget Compon

entQuantity Unit Total

Units Unit Cost Total Cost Total Cost

Year 1 Year 2 Year 3 Year 1 Year 2 Year 3 Year 1 Year 2 Year 3

1. Other Personnel Services (Project Staff) 12.1 12

Person-month 12 175 2,100 2,100

2. Ticket (Domestic) 32 27Participant-trip 27 250 6,750 6,750

3. Daily Subsistence Allowance 31 108

Participant-trip 108 80 8,640 8,640

4. Local transport 33 3 Trip 3 150 450 450 5. Fuel and utilities 52 3 Trip 3 100 300 300 6. Consumables 51 1 Package 1 150 150 150 7. Capital Equipment (Survey instruments) 42 1 Package 1 10,000 10,000 10,000 8. Report writing/Publication cost 63 1 Package 1 1,000 1,000 1,0009. Miscellaneous 67 1 Package 1 2,000 2,000 2,000

Subtotal Activity 1.1 31,390 30,390 1,000 0 0 0 0 0

1. Expert 11.2 12 12 12Person-month 36 250 9,000 3,000 3,000 3,000

2. Research Assistant 12.1 12 12 12Person-month 36 625 22,500 7,500 7,500 7,500

3. Daily subsistence allowance 31 24 24 24 Participant 72 80 5,760 1,920 1,920 1,9204. Local transport 33 24 24 24 Participant 72 150 10,800 3,600 3,600 3,6005. Consumables 51 1.5 1 0.5 Package 3 10,000 30,000 15,000 10,000 5,000

6. Other labor 12.3 12 12 12Person-month 36 250 9,000 3,000 3,000 3,000

7. 1 Capital Equipment (Genetic Facility) 42 1 Package 1 125,000 125,000 125,0007.2 Laboratory space and utilities (Genetics Lab) 41 1 1 1 Year 3 9,000 3,000 3,000 3,0008. Report writing/Publication cost 63 1 Package 1 1,000 1,000 1,0009. Miscellaneous 67 1 1 1 Package 3 500 1,500 500 500 500

Subtotal Activity 1.2 214,560 159,520 29,520 25,520 9,000 3,000 3,000 3,000

ITTO EA

Output 1. Gall rust resistant plus trees and gall rust resistant non-plus trees of F. moluccana selected, characterized, and conservedActivity 1.1. Survey of provenance, selection and collection of propagules and leaf samples of plus trees and gall rust resistant trees

Activity 1.2. Genetic characterization of gall rust resistant trees (plus trees and non-plus trees)

-20-

Page 26: ITTO · 2015. 10. 6. · international tropical timber organization . itto. project proposal . title: genetic conservation, utilization and management of gall rust resistant strains

Outputs and Activities Description

Budget Compon

entQuantity Unit Total

Units Unit Cost Total Cost Total Cost

1. Expert* 11.2 0 0 0Person-month 36 0 0 0 0 0

2. Research Assistant 12.1 12 12 12Person-month 36 625 22,500 7,500 7,500 7,500

3. Daily subsistence allowance 31 24 24 24 Participant 72 80 5,760 1,920 1,920 1,9204. Local transport 33 24 24 24 Participant 72 150 10,800 3,600 3,600 3,6005. Consumables 51 1.5 1 0.5 Package 3 10,000 30,000 15,000 10,000 5,000

6. Other labor 12.3 12 12 12Person-month 36 250 9,000 3,000 3,000 3,000

7.1 Capital Equipment (Genomic Facility) 42 1 Package 1 125,000 125,000 125,0007.2 Laboratory space and utilities (Genetics Lab) 41 1 1 1 Year 3 9,000 3,000 3,000 3,0008. Report writing/Publication cost 63 1 Package 1 1,000 1,000 10009. Miscellaneous 67 1 1 1 Package 3 3,000 9,000 3,000 3,000 3,000

Subtotal Activity 1.3 213,060 159,020 29,020 25,020 9,000 3,000 3,000 3,000

1. Expert 11.2 12Person-month 12 250 3,000 3,000

2. Other personnel services (Project staff) 12.1 12

Person-month 12 175 2,100 2,100

3. Other labor 12.3 12Person-month 12 175 2,100 2,100

3. Consumables 51 1 Package 1 1,000 1,000 1,0004. Report writing/Publication cost 63 1 Package 1 1,000 1,000 1,0005. Miscellaneous 67 1 Package 1 500 500 500

Subtotal Activity 1.4 9,700 9,700 0 0 0 0 0 0Subtotal Output 1 468,710 358,630 59,540 50,540 18,000 6,000 6,000 6,000

ITTO EA

Activity 1.3. Screening of candidate genes for gall rust resistance in F. moluccana

Activity 1.4 Determination of wood quality traits of gall rust resistant trees (plus trees and non-plus trees)

-21-

Page 27: ITTO · 2015. 10. 6. · international tropical timber organization . itto. project proposal . title: genetic conservation, utilization and management of gall rust resistant strains

Outputs and Activities Description

Budget Compon

entQuantity Unit Total

Units Unit Cost Total Cost Total Cost

1. Expert 11.2 12 12 12Person-month 36 250 9,000 3,000 3,000 3,000

2. International expert (CIRAD)/Daily Subsistence Allowance 31 10 10 10 Person -day 30 125 3,750 1,250 1,250 1,250

3. Ticket (International) 34 2 2 2Participant-trip 6 1,750 10,500 3,500 3,500 3,500

4. Accommodation 35 10 10 10Person-night 30 75 2,250 750 750 750

5. Research Assistant 12.1 12 12 12Person-month 36 625 22,500 7,500 7,500 7,500

6. Other labor 12.3 12 12 12Person-month 36 175 6,300 2,100 2,100 2,100

7. Consumables 51 1 Package 1 2,000 2,000 2,0008. Report writing/Publication cost 63 1 Package 1 1,000 1,000 1,0009. Miscellaneous 67 1 1 1 Package 3 500 1,500 500 500 500

Subtotal Activity 2.1 58,800 20,600 19,600 18,600 0 0 0 0

1. Expert 11.2 12 12 12Person-month 36 250 9,000 3,000 3,000 3,000

2. International expert (CIRAD)/Daily Subsistence Allowance 31 10 10 10 Person -day 30 125 3,750 1,250 1,250 1,250

3. Ticket (International) 34 2 2 2Participant-trip 6 1,750 10,500 3,500 3,500 3,500

4. Accommodation 35 10 10 10Person-night 30 75 2,250 750 750 750

2. Technician 12.2 12 12 12Person-month 36 250 9,000 3,000 3,000 3,000

3. Other labor 12.3 12 12 12Person-month 36 175 6,300 2,100 2,100 2,100

4. Consumables 51 1 1 1 Package 3 1,000 3,000 1,000 1,000 1,0005. Other consumables 54 1 1 1 Package 3 1,000 3,000 1,000 1,000 1,0006. Report writing/Publication cost 63 1 Package 1 1,000 1,000 1,0007. Miscellaneous 67 1 1 1 Package 3 500 1,500 500 500 500

Subtotal Activity 2.2 49,300 16,100 17,100 16,100 0 0 0 0

Activity 2.2. Characterization and identification of effective mycorrhiza and rhizobium-root associations; Inoculation of clones with mycorrhizal fungi and rhizobial bacteria, and assessment of its effect in

Activity 2.1. Refinement of protocols for macro-and micropropagation and mass production of clones

ITTO EA

Output 2. Clonal orchard and clonal plantation of F. moluccana established.

-22-

Page 28: ITTO · 2015. 10. 6. · international tropical timber organization . itto. project proposal . title: genetic conservation, utilization and management of gall rust resistant strains

Outputs and Activities Description

Budget Compon

entQuantity Unit Total

Units Unit Cost Total Cost Total Cost

1. Expert 11.2 12 12 12Person-month 36 250 9,000 3,000 3,000 3,000

2. International expert (CIRAD)/Daily Subsistence Allowance 31 10 10 10 Person -day 30 125 3,750 1,250 1,250 1,2503. Daily subsistence allowance 31 24 24 24

Participant-trip 72 30 2,160 720 720 720

5. Consumables 51 1 1 1 Package 3 1,000 3000 1000 1000 1000

6. Other labor 12.3 12 12 12Person-contract 36 175 6,300 2,100 2,100 2,100

7. Report writing/Publication cost 63 1 Year 1 1,000 1,000 1,000 8. Miscellaneous 67 1 1 1 Package 3 500 1,500 500 500 500

Subtotal Activity 2.3 26,710 8,570 8,570 9,570 0 0 0 0

1. Expert 11.2 48 48 48Person-month 144 250 36,000 12,000 12,000 12,000

2. Other Personnel Services (Project staff) 12.1 12 12 12

Person-month 36 175 6,300 2,100 2,100 2,100

3. Labor 12.3 48 48 48Person-month 144 250 36,000 12,000 12,000 12,000

3. Ticket (Domestic) 32 27Participant-trip 27 250 6,750 6,750

4. Daily Subsistence Allowance 31 108

Participant-trip 108 80 8,640 8,640

5. Local transport 33 3 3 3 Trip 9 150 1,350 450 450 4506. Consumables 51 1 1 1 Package 3 4,000 12,000 4,000 4,000 4,0007. 1 Capital Equipment (Nursery ) 42 1 Package 1 20,000 20,000 20,0007.2 Nursery space and utilities (Nursery) 41 1 1 1 Year 3 21,600 7,200 7,200 7,2008. Fuel and utilities 52 2 2 2 Year 6 200 1,200 400 400 4009. Report writing/Publication cost 63 1 Package 1 1,000 1,000 1,00010. Miscellaneous 67 1 1 1 Package 3 500 1,500 500 500 500

Subtotal Activity 2.4 130,740 66,840 31,450 32,450 21,600 7,200 7,200 7,200

ITTO EA

Activity 2.3 Formulation of biocontrol protocol to combat gall rust

Activity 2.4. Development of appropriate nursery techniques and clonal seed orchard establishment

-23-

Page 29: ITTO · 2015. 10. 6. · international tropical timber organization . itto. project proposal . title: genetic conservation, utilization and management of gall rust resistant strains

Outputs and Activities Description

Budget Compon

entQuantity Unit Total

Units Unit Cost Total Cost Total Cost

1. Expert 11.2 24Person-month 24 250 6,000 6,000

2. Other Personnel Services (Project staff) 12.1 24

Person-month 24 175 4,200 4,200

3. Labor 12.3 72Person-month 72 250 18,000 18,000

3. Ticket (Domestic) 32 12 Trip 12 250 3,000 3,0004. Daily Subsistence Allowance 31 72

Participant-trip 72 80 5,760 5,760

5. Local transport 33 3 Trip 3 150 450 4506. Consumables 51 1 Package 1 4,000 4,000 4,000

7. Capital Equipment (Clonal seed orchard establishment ) 42 1  Package 1 40,000 40,000 40,0008. Clonal seed orchard (Land and building) 42 21,000 10,500 10,5009. Vehicle use 42 9,000 4,500 4,50010. Equipment 3,000 1,500 1,500

11. Community coordination and meetings and workshops 65 6,000 3,000 3,00012. Fuel and utilities 52 2 Year 2 200 400 40013. Report writing/Publication cost 63 1 1 500 500 50014. Miscellaneous 67 1 Package 1 500 500 500

Subtotal Activity 2.5 82,810 0 0 82,810 39,000 0 19,500 19,500Subtotal Output 2 348,360 112,110 76,720 159,530 60,600 7,200 26,700 26,700

Activity 2.5 Clonal plantation established in tree farmer area

ITTO EA

-24-

Page 30: ITTO · 2015. 10. 6. · international tropical timber organization . itto. project proposal . title: genetic conservation, utilization and management of gall rust resistant strains

Non-activity based expenses

Personnel (20% salary of UPLB-based project management team (equivalent to the time devoted to project) 11 12 12 12 63,000 21,000 21,000 21,000

Program coordinator 11.1 12 12 12 Person-month 36 250 9,000 3,000 3,000 3,000

Project secretary 11.3 12 12 12 Person-month 36 250 9,000 3,000 3,000 3,000

Ticket (International; Conference representation, i.e Oral or Poster presentation of research results) 34 8 7 Participant 15 3,750 56,250 18,750 18,750 18,750

Office space and utilities 41 1 1 1 Year 3 14,400 4,800 4,800 4,800

Capital Equipment (Computer equipment and peripherals) 42 1 Package 1 5,000 5,000 5,000

Office supplies 53 2 2 2 Package-Year 6 500 3,000 1,000 1,000 1,000

Other Consumables 54 1 1 1 Year 3 3,000 1,000 1,000 1,000 Sundry 61 1 1 1 Year 3 500 1,500 500 500 500 Local transport 33 4 4 4 Year 12 500 6,000 2,000 2,000 2,000 Fuel and utilities 52 4 4 4 Year 12 500 6,000 2,000 2,000 2,000 3,000 1,000 1,000 1,000 Other publication cost 63 5 3 Package 8 500 3,500 2,500 1,000

Project Steering Committee Meeting (transport and organization) 65 2 2 2

Meeting-Year 6 875 5,250 1,750 1,750 1,750

Consultative meeting (transport and organization) 65 1 2 2

Meeting-Year 5 500 2,500 500 1,000 1,000

Project management team (Technical Meeting) 65 2 2 2

Meeting-Year 6 1,000 6,000 2,000 2,000 2,000

Monitoring and evaluation (Internal M&E of Project Team) 64 2 2 2 Year 6 300 1,800 600 600 600

Other Miscellaneous 67 1 1 1 Year 3 1,000 3,000 1,000 1,000 1,000 300 100 100 100

-25-

Page 31: ITTO · 2015. 10. 6. · international tropical timber organization . itto. project proposal . title: genetic conservation, utilization and management of gall rust resistant strains

Subtotal Non-activity based expenses 117,800 41,100 39,100 37,600 83,700 27,900 27,900 27,900 SUBTOTAL 1 (1) 934,870 511,840 175,360 247,670 162,300 41,100 60,600 60,600

Project Administrative Cost by the UPLB Foundation, Inc. (15% of 10-60 budget component, excluding EA fund requested )

62 1 1 1 Year 3 140,231 76,776

26,304

37,151

SUBTOTAL 1 (2) 1,075,101 588,616 201,664 284,821 162,300 41,400 60,600 60,600 Executing Agency Cost 71 162,300

162,300

Monitoring and review Costs (effective estimation) 81 25,000

Evaluation Costs (effective estimation) 82 15,000

SUBTOTAL 2 40,000

Program Support Costs (12% of 10-82 above) 83 133,812

GRAND TOTAL 1,411,213

-26-

Page 32: ITTO · 2015. 10. 6. · international tropical timber organization . itto. project proposal . title: genetic conservation, utilization and management of gall rust resistant strains

3.4.2 Consolidated budget requested from ITTO by component (in US$) Category Description Total Year 1 Year 2 Year 3

10 Personnel

11 National Experts

11.1 Project coordinator

9,000

3,000

3,000

3,000

11.2 Experts

81,000

27,000

24,000

30,000

11.3 Project secretary

9,000

3,000

3,000

3,000 12 Other personnel

12.1 Other personnel services (Project staff)

14,700

6,300

2,100

6,300

12.1 Research Assistant

67,500

22,500

22,500

22,500

12.2 Technicians

9,000

3,000

3,000

3,000

12.3 Other labor/Labor

93,000

26,400

24,300

42,300 19 Component Total 283,200 91,200 81,900 110,100 30 Travel

31 Daily subsistence allowance 47,970 25,590 8,310 14,070 32 Ticket (Domestic) 16,500 13,500 0 3000 33 Local transport 29,850 10,100 9,650 10,100 34 Ticket (International) 77,250 25,750 25,750 25,750 35 Accommodation 4,500 1,500 1,500 1,500 39 Component Total 176,070 76,440 45,210 54,420 40 Capital Items

41 4.1 Use of laboratory space and utilities

4.2 Use of space and utilities (Nursery)

4.3 Office space and utilities 42 Capital equipment

42.1 Survey instruments 10,000 10,000 0 0 42.2 Genetic Facility 125,000 125,000 0 0 42.3 Genomic Facility 125,000 125,000 0 0 42.4 Nursery Facility 20,000 20,000 0 0

42.5 Clonal Seed Orchard Establishment Facility

40,000 0 40,000

42.6 Computer equipment and peripherals) 5,000 5,000 0 0

49 Component Total 325,000 285,000 0 40,000 50 Consumable items

51 Consumables (Raw materials)

85,150

39,150

26,000

20,000

52 Fuel and Utilities

7,900

2,700

2,400

2,800

53 Office supplies

3,000

1,000

1,000

1,000

54 Other consumable items

3,000

1,000

1,000

1,000 59 Component Total 99,050 43,850 30,400 24,800

-27-

Page 33: ITTO · 2015. 10. 6. · international tropical timber organization . itto. project proposal . title: genetic conservation, utilization and management of gall rust resistant strains

Category Description Total Year 1 Year 2 Year 3 60 Miscellaneous

61 Sundry

1,500

500

500

500

62

Project Administrative Cost by UPLB Foundation, Inc. (15% of 10-60 budget component, excluding EA Budget)

140,231

76,776 26,304 37,151

63 Other publication cost

12,000

1,000

5,500

5,500

64 Monitoring and evaluation (Internal M&E of Project Team)

1,800

600

600

600

65 Meetings (Transport and Organization)

Project Steering Committee Meeting (transport and organization)

5,250

1,750

1,750

1,750

Consultative meeting (transport and organization)

2,500

500

1,000

1,000

Project management team (Technical Meeting)

6,000

2,000

2,000

2,000

Community coordination and meetings and workshops - - - -

67 Miscellaneous

22,500

9,000

6,500

7,000 69 Component Total 191,781 92,126 44,154 55,501

Total 10-60 Above 1,075,101 588,616 201,664 284,821 70 National Management Cost

71 Executing Agency Cost 162,300 41,100 60,600 60,600 79 Component Total 162,300 41,100 60,600 60,600

SUBTOTAL 1 1,237,401 629,716 262,264 345,421

80 ITTO Monitor, Evaluation Costs

81 Monitoring and Review Costs (effective estimation) 25,000

82 Evaluation Costs (effective estimation) 15,000

SUBTOTAL 2 40,000

83 Program Support Costs (12% of 10-82 above)

133,812

84 Donor Monitoring Costs -

89 Component Total 173,812

90 Refund of Pre-Project Costs - GRAND TOTAL 1,411,213

-28-

Page 34: ITTO · 2015. 10. 6. · international tropical timber organization . itto. project proposal . title: genetic conservation, utilization and management of gall rust resistant strains

3.4.3 Consolidated budget by component to be requested from ITTO Category Description Total Year 1 Year 2 Year 3

10 Personnel 283,200 91,200 81,900 110,100 20 Duty Contracts

30 Travel 176,070 76,440 45,210 54,420

40 Capital Items 325,000 285,000 40,000

50 Consumable items 99,050 43,850 30,400 24,800

60 Miscellaneous 191,780.5 92,126 44,154 55,500.5

SUBTOTAL 1 1,075,101 589,616 201,664 284,820.5

80 ITTO Monitor, Evaluation Costs

81 Monitoring and Review Costs (effective estimation) 25,000

82 Evaluation Costs (effective estimation) 15,000

SUBTOTAL 2 40,000 83 Program Support Costs (12%

of 10-82 above) 133,812 84 Donor Monitoring Costs

90 Refund of Pre-Project Costs ITTO TOTAL 1,248,913

3.4.4 Consolidated budget by component to be requested from the executing and collaborating

agencies (in kind/valued in US$) Category Description Total Year 1 Year 2 Year 3

10 Personnel (20% salary of UPLB-based project management team (equivalent to the time devoted to project)

63,000 21,000 21,000 21,000

19 Component Total 63,000 21,000 21,000 21,000 40 Capital Items

41 Office space and utilities 14,400 4,800 4,800 4,800 42 Nursery space and utilities 21,600 7,200 7,200 7,200 43 Laboratory space and utilities 18,000 6,000 6,000 6,000 44 Equipment 3,000

1,500 1,500

45 Clonal seed orchard (Land and building) 21,000

10,500 10,500

49 Component Total 78,000 18,000 30,000 30,000 50 Consumable items 52 Fuel and utilities 3,000 1,000 1,000 1,000 54 Other consumable items 3,000 1,000 1,000 1,000 55 Vehicle use 9,000

4,500 4,500

59 Component Total 15,000 2,000 6,500 6,500 60 Miscellaneous 67 Other Miscellaneous 300 100 100 100

68 Community coordination meetings and workshops 6,000

3,000 3,000

69 Component Total 6,300 100 3,100 3,100

GRAND TOTAL 162,300 41,100 60,600 60,600

-29-

Page 35: ITTO · 2015. 10. 6. · international tropical timber organization . itto. project proposal . title: genetic conservation, utilization and management of gall rust resistant strains

3.5 Assumption, risks, and sustainability Since the goal of this objective is to uplift the socio-economic condition of the local tree-farmers from enhanced yield of plantations of F. moluccana, thereby promoting active economic activity in the region, the program assumes acceptance and full support from all stakeholders. However, the long-term realization of this goal can be achieved if there is a continuous support of the government through the DENR-CARAGA and local government units. The roles of the UPLB-CFNR and DENR-CARAGA will be very important to maintain initiated activities of this program, particularly the proposed nursery and clonal orchards, and protect the information to be generated by the program for possible exploitation. These will become possible if the UPLB-CFNR and DENR-CARAGA will enter into an agreement of a long-term partnership in providing support funds for maintaining the clonal seed orchards of F. moluccana. Risks

- Lack of control on the distribution and use of the products of the improved clones; example, selling of seeds or vegetative parts from clones. To protect the produced F. moluccana clones, the EA will require a material transfer agreement with the buyer of the clones. If the protocol for production will be bought by a company, a royalty fee from every sale of the clones will be sought, which will be used for the maintenance and production seed orchards.

- No full support from major project stakeholders especially DENR-CARAGA and tree farmers

To avoid this, early and continuous coordination with the primary stakeholders, especially farmers. They will be part of the entire management cycle i.e. project design, operations planning, implementation, and monitoring and evaluation. The full benefits of the project from the environmental and socio-economic perspectives will be fully explained – environmental –tree farmers will be encouraged to plant in denuded areas; and socio-economic – increase in income will improve their overall well being.

-30-

Page 36: ITTO · 2015. 10. 6. · international tropical timber organization . itto. project proposal . title: genetic conservation, utilization and management of gall rust resistant strains

PART 4. IMPLEMENTATION ARRANGEMENT 4.1 Organization structure and stakeholder involvement mechanism 4.1.1 Executing/Implementing agency and partners The profile of the CFNR-UPLB as the implementing agency is indicated in Annex 1. 4.1.2 Project Management team The structure of the Project Management Team is shown by the figure below and its composition is listed shown in Annex 2.

Program Coordinator

(UPLB)

Project Steering Committee

ProgramSecretary

Project Leader

(Activity 1.3)

Project Leader

(Activity 1.4)

Project Leader

(Activity 1.2)

Project Staff

&other labor

Project Leader

(Activity 2.1)

Project Leader

(Activity 2.2)

Project Leader

(Activity 2.3

Project Leader(Activity 2.4) UPLB SIte

International Experts(CIRAD)

Project Staff

&other labor

Research Assistant

&other labor

Research Assistant

&other labor

Laboratory Technician

&other labor

Research Assistant

&other labor

S&T Consultant

Cooperating Agency(DENR-CARAGA)

(Activity 1.1, 2.4 (CARAGA Site), 2.5)

Project Staff

&other labor

Project Leader

(Activity 2.5)

Structure of the Project Management Team. Bold lines indicate direct relationship and broken lines indicate interconnectivity of projects. The Project Management Team will be headed by the Program Coordinator (PC). The PC will be in close coordination with two coordinating agencies, CIRAD and DENR-CARAGA. The PC will also work closely with the Project Steering Committee (PSC), with roles indicated in Section 4.1.3. There will be nine projects. The PC, aside from overseeing the whole program, will lead the Activity 1.3 (screening for candidate genes for gall rust resistance). Project leaders of activities 1.2 (genetic characterization of gall rust-resistant plus trees and non-plus trees) and 1.3 will work closely with each other and will comprise the molecular component of the program. Project leader of activities 2.1 (micro- and macropropagation/clone production) and 2.2 (mycorrhiza and rhizobium screening and inoculation), and 2.2 and 2.3 (biocontrol formulation) will work closely with each other since they will be using the same materials for the project. The project leaders of activities 2.1, 2.2, and 2.3 will be coordinating with the international experts from CIRAD. As described in the stakeholder analysis, CIRAD will primarily be involved in these three major activities, although they will be assisting the Philippine team in the survey of gall rust resistant plus trees as well as with the molecular component of the project. Project leader of activity 2.4 (nursery and clonal seed orchard establishment) will coordinate with project leaders of activities 2.1 and 2.2 because activity 2.4 will use materials produced and assessed in activities 2.1 and 2.2, respectively. Since clonal nurseries and orchards will be established both in the CARAGA region and UPLB, the Project leaders of both teams will be coordinating with each other as well as with the Project leader of activity 2.5, especially during the implementation of the clonal plantation in tree farmers area. Science and Technology consultants will be involved in the genomic component of the project. Respective research assistant or laboratory technician or project staff (UPLB or DERN-CARAGA staff) will work under the direct supervision of their respective Project leaders. For all administrative matters and concerns the Project Secretary will work under the direct supervision of the program coordinator. The terms of reference for the composition of the Project Management Team are listed in Annex 3.

-31-

Page 37: ITTO · 2015. 10. 6. · international tropical timber organization . itto. project proposal . title: genetic conservation, utilization and management of gall rust resistant strains

Although not part of the Project management structure, the Program Coordinator will work closely with the UPLB Foundation Inc., a non-profit, non-stock private corporation registered with the Philippines Securities and Exchange Commission (SEC), nominated in this program proposal to manage the funds requested from the ITTO. It is a Science Foundation duly accredited and certified by the Department of Science and Technology and by the Philippine Council for NGO Certification. Based inside the UPLB campus, UPLBFI, thru its quality assurance procedures and services, ensures that the contractual obligations with the funding agency are promptly fulfilled and the necessary technical and administrative backstopping supports to the project are provided. UPLBFI provides the adequate and efficient administrative support and management system necessary for the successful implementation of the project. It is responsible for the disbursement and accounting of project funds. Project Management Officer of UPLBFI provides guidance and directions to the project team during the implementation period. It is responsible for the hiring of project personnel and for the termination upon completion or termination of the project. UPLBFI ensures the orderly, systematic and timely implementation of the project as programmed and approved; provides the necessary administrative assistance such as financial management, accounting services, communications, and records management; ensures the regular monitoring on the progress of the project; and ensures the recording and reporting of any unforeseen changes in conditions that affected the implementation of the project.

Gross Administrative fee As service fee for the management of project funds, UPLBFI charges a project administrative cost of 15% of the total budget of the project under its management. Policies related to this administrative cost are explained below. The administrative fees accumulated in a year constitute the gross income of the Foundation in that year.

Pre-implementation expenses It is the policy of the Foundation to allocate 15% of the gross administrative fee for paying pre-implementation expenses. These expenses correspond to the cost of time, effort, and resources invested by an individual or group of individuals or institution in connection with the search of a project, crafting of the project proposal, negotiation with the funding agency, which pave the way to clinching a project for implementation through UPLBFI. Pre-implementation expenses are categorized and paid out as follows; a) Finder’s fee – 7.5% as payment to an individual, or group of individuals, or unit/institution/organization for identifying, packaging, and negotiating the project, and b) Project development fee – 7.5% as payment to an individual, group of individuals, or institution/organization for preparing the project proposal. Sharing of the Net Administrative Fee (NAF) The NAF is the remainder of the administrative fee after paying the project pre-implementation expenses, that is, finder’s fee and project development fee and other related costs. The accumulated NAF at the end of the Fiscal Year is shared and utilized pursuant to the General Policy of the Administrative Income from UPLBFI Projects. Under this policy, the principles and premises are; 1) UPLBFI, as a Science Foundation duly accredited by the Department of Science and Technology (DOST), is mandated to allocate 60% of its income to UPLB. This is pursuant to the meaning of Section 24 of the Republic Act 2067 as amended by RA 3589 (An Act to Integrate, Coordinate, and Intensify Scientific and Technological Research and Development and to Foster Invention; To Provide Funds Therefor; and for Other Purposes or the ‘Science Act of 1958’), and 2) As provided by the Memorandum of Agreement (MOA) between UPLB and UPLBFI in January 2006 (Please see attached Supplementary material 2.1-2.5), the income of UPLBFI out of the Administrative cost charges (15%) for each project will be shared according to the following schedule: 40% goes to UPLBFI to sustain its operation (20% for maintenance and operation and 20% for capital build up fund) and the 60% goes to UPLB (20% for Central Administration – UPLB, 20% for the concerned college – in the case of this proposal it is the College of Forestry and Natural Resources or CFNR, and 20% for the implementing unit – Department of Forest Biological Sciences or FBS-CFNR). 4.1.3 Project steering committee The Project Steering Committee (PSC) will be formed, consisting of the Dean of the CFNR-UPLB who will act as the chair of the committee, the Regional Technical Director of DENR-CARAGA, the head of the Forest

-32-

Page 38: ITTO · 2015. 10. 6. · international tropical timber organization . itto. project proposal . title: genetic conservation, utilization and management of gall rust resistant strains

Management Bureau of DENR, the Program Coordinator, and a member representative from the ITTO. The University of the Philippines System has a special steering committee for genetics and genome researches in agriculture, biotechnology, and forestry called the Philippine Genome Center (PGC). In UPLB, the College of Forestry and Natural Resources is a member unit of the PGC-UPLB, which is headed by a Program Coordinator. Thus, the Program Coordinator of PGC-UPLB will also be appointed in the PSC. The PSC will guide, evaluate, and provide advice about the direction of the implementation of all component projects. The PSC will also review the financial status of the program and suggests changes in the budget component if necessary. 4.1.4 Stakeholder involvement mechanism An initial meeting between UPLB-CFNR project team and the collaborating agencies (CIRAD and DENR-CARAGA) will be organized before the operational activities of the projects. A representative from the CARAGA FTFDI, Falcata tree grower/cooperators of DENR-CARAGA, and Philippine Wood Producers Association will also be invited during the meeting. Technical meetings will be organized every six months to evaluate the progress of the activities according to the work plan. A consultative meeting to be coordinated by DENR-CARAGA with the respective local government units (LGUs) of CARAGA and tree farmers will be conducted before the deployment of mass propagated planting materials for plantation establishment. Impacts of the project on gender equity

The main composition of the project management team is comprised of almost equal number of male and female researchers (Please see Annex 3). Field-based personnel, including labor, will also be given equal opportunity of employment for both male and female. Women representation in the stakeholder composition, including their views, will also be sufficiently represented.

Since the project will be working with community-organized groups, gender sensitization will be advocated. Since one of the project outcomes is to alleviate poverty in the CARAGA region, women will have more opportunities. 4.2 Monitoring, review, and evaluation Monitoring, review, and evaluation will be made to ensure that the documentation, logistics, and inter-governmental arrangements and linkages are properly placed. The following reports will be submitted in accordance with the requirements of the ITTO. Inception report To be submitted after signing the agreement, confirmation of availability of office, nursery, and laboratory space, registration of bank account for fund transfer, and preparation of necessary legal documents. Yearly operation reports The yearly operation report, which contains the budget plan in accordance with the work plan for the current year, will be submitted prior to the operation of the activities of the current year and for endorsement by the ITTO. The first yearly operation report will be submitted together with the inception report. Project progress reports To be submitted to the ITTO annually or as often as required by the ITTO. The reports contain information on the executed activities and achieved outputs during the period covered by the report, including copies of research paper(s), abstract(s), or proceeding(s) of scientific meetings, which used results of the project. Technical reports The technical report will contain technical and scientific data, analyses of data, and project results, which may be obtained from one activity or a set of activities, including scientific papers. This report will be submitted two months after project completion. Financial report Financial report will be prepared and audited by the UPLB Foundation Inc., as the administrative agency. This report will be submitted within three months after the end of the current financial report.

-33-

Page 39: ITTO · 2015. 10. 6. · international tropical timber organization . itto. project proposal . title: genetic conservation, utilization and management of gall rust resistant strains

Completion report The completion report will contain the activities, inputs and expenditures, and achieved outputs, including copies of relevant research paper(s), abstract(s), or proceeding(s) of scientific meetings produced during the entire implementation of the program. The report will also contain lessons learned from the implementation of the projects. This report will be prepared and submitted to the ITTO at least three months after the completion of the project. Monitoring and review Review, monitoring, and evaluation of the project will also be conducted using ITTO procedures. The PSC meeting will be used to review and evaluate the project implementation. 4.3 Dissemination and mainstreaming of project learning 4.3.1 Dissemination of project results Technical reports The technical report, which contains scientific data and any other important information, will be disseminated to relevant institutions such as DENR, UPLB, and local government units of CARAGA. National workshops and internal meetings National and international scientific meetings will be used to disseminate project results. Completion report This report contains the complete information of project implementation, results, and findings. This report will be submitted to ITTO for wider uses and dissemination. 4.3.2 Mainstreaming of project results The primary users of the findings and results of this project are local community, local government, and research and academic institutions. Through dissemination for wide application and uses of the project findings and results, the expected impacts of the whole program will be realized in conservation and breeding program of F.moluccana species in the Philippines.

-34-

Page 40: ITTO · 2015. 10. 6. · international tropical timber organization . itto. project proposal . title: genetic conservation, utilization and management of gall rust resistant strains

ANNEX 1. Profile of the Executing Agency/ Implementing Agency The College of Forestry and Natural Resources (also referred to as CFNR) is one of the 11 degree-granting units of the University of the Philippines Los Baños (UPLB). It started as the Forest School under the UP College of Agriculture in 1910, making it the oldest forestry school in the Philippines. CNFR is considered one of the nation’s finest and has been identified as a "Center of Excellence" in forestry education by the Philippine Commission on Higher Education in 2000. Its vision is anchored on five-point strategic programs namely, 1) advancing distinctive excellence in forests and natural resources education, 2) developing world-class research and development capacity, 3) instituting responsive extension services for community and national development, 4) promoting enabling environment through good governance and effective support system, 5) generating resources for productivity. The research program undertaken by the CFNR is in line with the college’s vision and thrust which supports the overall program thrust and the national government’s focus. The College remains to be at the forefront of teaching, research and extension activities in various areas such as climate change, silviculture, ecology, biodiversity, physiology, agroforestry, pulp and paper technology, natural resource management, policy formulation, economics, and community development. CFNR’s 59 faculty members, who are among the top professionals in their fields, are very dedicated in advancing the science and practice of forestry and natural resources management through responsive instruction that is sensitive to the needs of the students. The CFNR R&D programs strategically revolve in areas such as biodiversity conservation, forest biotechnology, environmental forestry, silviculture, forest resources management, forest restoration, forestry economics, forest policies, forest products utilization, pulp and paper science, agroforestry, natural resources conservation and management, geo-spatial technology, social forestry and governance, mountain ecosystem development, watershed management, biofuels, carbon sequestration and climate change. The CFNR is one of the seven degree-granting units in the University of the Philippines Los Baños, as well as the second oldest among them. It is headed by a Dean and assisted by an Associate Dean. The college has one institute and three (3) academic departments viz., the Institute of Renewable and Natural Resources (IRNR), the Department of Forest Biological Sciences, the Department of Forest Products and Paper Science (FPPS) and the Department of Social Forestry and Forest Governance (SFFG). These departments administer the courses offered by the college and develop formal courses at the undergraduate (BS) and graduate levels (MSc, PhD). At the same time, they undertake research and creative work that contribute to the advancement of forestry and environmental science and technology in the country. Academic Units 1. Forest Products and Paper Science (FPPS)

The department is one of the oldest CFNR units. Its mandate is to provide instruction and carry out research and extension in the areas of wood science and forest products utilization. In this time of limited forest resources, the Department focuses research on non-timber forest products, wood protection, bio-based composites, pulp and paper, and conservation of wood resources through efficient utilization.

2. Social Forestry and Forest Governance (SFFG) As the pioneering academic institution in the field of Social Forestry and Forest Governance, the department is an astute advocate of participatory, equitable and sustainable natural resources management. It envisions to advance the frontier of knowledge through the highest standards of education, research and extension.

3. Institute of Renewable Natural Resources Established in 1998, the Institute is envisioned as a center of excellence in natural resources management science and a dynamic leader in the integrated and sustainable management of renewable natural resources.

4. Forest Biological Sciences The department, established in 1965, is envisioned to be the learning and research center in forest biological sciences, biodiversity, biotechnology, and allied fields for the conservation and sustainable use of biological resources for the better quality of life and environment.

-35-

Page 41: ITTO · 2015. 10. 6. · international tropical timber organization . itto. project proposal . title: genetic conservation, utilization and management of gall rust resistant strains

Centers 1. Makiling Center for Mountain Ecosystems (MCME) MCME is the first center in the Philippines to focus on the science and importance of mountains. Based on the approved BOR resolution which took effect on January 30, 2003, the revised mandate of the MCME are to: 1) assess critical issues relating to the conservation of Mt. Makiling and development programs to effectively deal with them; 2) generate resources, formulate and execute plans and strategies, monitor and coordinate the implementation of projects and activities for the sustainable management of Mt. Makiling Forest Reserve; and 3) conduct research and demonstration programs on mountain ecosystems development. 2. Training Center for Tropical Resources and Ecosystems Sustainability (TREES) The Center was established in June 1998 by the UP Board of Regents and has a satellite office in Baguio. TREES is the extension/training unit of CFNR and is mandated to package and implement relevant and continuing education courses in sustainable tropical resources and ecosystems management 3. Forestry Development Center (FDC) The Center, established in 1978, is mandated to conduct basic policy researches in forestry and develop or help develop effective machinery for forestry policy formulation and implementation. Institute Institute of Agroforestry (IAF) The IAF envisions forming progressive and productive communities where farming is harmonized with environmental conservation principles for the sustained production of food, wood and provision of services through the use of sound agroforestry practices. Personnel As of 2012, the CFNR has a total of 200 personnel consisting of 59 (29%) faculty members, 39 (20%) research and extension personnel (REPS), and 102 (51%) administrative staff. Of the total 59 faculty members, 37 (63%) are Ph.D. degree holders, 19 (32%) are masters degree holders and 3 (5%) are BS degree holders. REPS consist of 39 (20%) PhD holders, 7 (18%) MS holders, and 11 (28%) BS degree holders. The administrative personnel consist of 101 (99%) administrative staff on regular appointments and 1 (1%) laborer on casual basis.

-36-

Page 42: ITTO · 2015. 10. 6. · international tropical timber organization . itto. project proposal . title: genetic conservation, utilization and management of gall rust resistant strains

ANNEX 2. Task and Responsibilities of key personnel

Name/Designation Education/Expertise Roles in project implementation Dr. Marilyn O. Quimado, University Researcher II CFNR-UPLB

PhD Plant Biotechnology

National Expert and Project Leader for Activity 2.1

Dr. Lerma S. Maldia,* Asst. Prof. CFNR-UPLB

PhD Agricultural Science (Phylogenetics & Phylogeography)

National Expert, Project Leader for Activity 1.3, and Program Coordinator

Dr. Jorge Gil C. Angeles University Researcher II, Philippine Genomic Center

PhD Molecular Biology (Minor in Bioinformatics)

Science and Technology Consultant for Activity 1.3

Dr. Rita P. Laude Professor Institute of Biological Sciences

PhD Science and Technology Consultant

Dr. Olivier Monteuuis, CIRAD

PhD International Expert and Lead of the CIRAD Team, To be Involved in Activities 2.1, 2.2, 2.3

Dr. Antoine Galiana, CIRAD

PhD International Expert, To be involved in Activities 2.1, 2.2, 2.3

Dr. Dennis Gilbero Science Research Analyst ERDS-DENR Region 13

PhD Forestry To be involved in Activity 1.1

Dr. Willie P. Abasolo, Prof. CFNR-UPLB

PhD Agricultural Science (Wood Engineering)

National expert and Project Leader for Activity 1.4

Prof. Crusty E.Tinio Asst. Prof. CFNR-UPLB

MSc Forest Genetics Project Leader for Activity 1.2

Prof. Mutya Ma. Q. Manalo, Associate Prof. CFNR-UPLB

MSc Microbiology National expert and Project Leader for Activity 2.3

Prof. Jessa P. Ata, Asst. Prof. CFNR-UPLB

MSc Forestry (Forest Pathology/Mycology)

Project Leader for Activity 2.2

Prof. Marlo Mendoza, Asst. Prof. CFNR-UPLB

MSc Forestry National Expert and Project Leader for Activity 2.4

Prof. Rosalie Calapis, Asst. Prof. CFNR-UPLB

MSc Forestry (Forest Products and Paper Science)

Support staff for Activity 1.4

For. Amelita C. Luna, University Researcher II CFNR-UPLB

MSc Forestry (Silviculture) Support staff for Activity 2.4

For. Ronnie Salac, Regional Technical Director DENR-CARAGA

BSc Forestry

Lead of the DENR-CARAGA Team National Expert for Activity 1.1, 2.4, and 2.5

For. Teresa Salisid, Forester, DENR-CARAGA

MSc Forestry Support staff for Activity 2.4

*Please see attached one-page Curriculum Vitae of the Program Coordinator

-37-

Page 43: ITTO · 2015. 10. 6. · international tropical timber organization . itto. project proposal . title: genetic conservation, utilization and management of gall rust resistant strains

ANNEX 3. Term of references of personnel and consultants funded by ITTO 1. Terms of references for Program Coordinator The Program Coordinator (PC) shall lead the program and coordinate the operational activities of the whole program. The PC shall be responsible for the day to day management of the project. The PC shall coordinate and supervise all activities, ensure that the objectives are attained, integrate all activities of the program, and oversee the preparation of all reports required by the ITTO. The PC shall work closely with the PSC, with the collaborating agencies, and with other personnel of each component project. Qualification, duration of contract, and payment: Postgraduate degree in forestry (with expertise in forest genetics and biotechnology, and with knowledge

in silviculture) Possess good English, both oral and written The PC will be hired for the duration of 36 months 2. Term of references for Program secretary The secretary shall assist the program coordinators in all administrative works and will be hired for the duration of 36 months. The secretary will report directly to the PC. Responsibilities: The secretary will be responsible in handling administrative functions, including preparation of the various reports by the project. He or she will work closely to all parties and personnel involved in the project, particularly with PC and experts. Qualification, duration of contract and payment: Graduate degree from any discipline preferably with minimum 2 years of working experience Possess good English, both oral and written Familiar and have good knowledge on Microsoft platform 3. Terms of references for National Expert(s) Responsibilities: The expert shall properly design the best appropriate approaches to achieve all outputs in activities. Expert shall prepare one or more technical reports related to his/her assigned activity. The reports and other database must be presented during the project management team meetings. Qualification, duration of contract and payment: Postgraduate degree The expert(s) shall hold expertise and knowledge in the field related to the assigned activity. Possess good English, oral and written. The project expert(s) will be hired for the duration of contract for each activity.

-38-

Page 44: ITTO · 2015. 10. 6. · international tropical timber organization . itto. project proposal . title: genetic conservation, utilization and management of gall rust resistant strains

ANNEX 4. Overall assessment and specific recommendations of the 49th Expert Panel PD 771/15 (I) Genetic Conservation, Utilization and Management of Gall Rust Resistant Strains of

Paraserianthes Falcataria Growing in the Philippines (Philippines) Assessment by the Forty-ninth Panel A) Overall Assessment The Panel acknowledged the relevance of the proposal to the Philippines and the importance to promote biotechnology to hasten tree improvement to meet the increasing demand for wood and its derived products. The panel also noted that the project proposal aims to conserve the disease resistant plus trees of P. falcataria. However, reformulation and clearer explanations to several aspects of the proposal are indispensable to undertake in order to understand the links between clones production, established plantation and end-use of the wood. The Panel also noticed information lacking on state-of-science to combat Gall Rust, land status for plantation, ownership of plantation and its beneficiaries. The Panel requested a supporting letter from the collaborating agency, CIRAD, to be included in the revision of the project proposal. B) Specific Recommendations 1. Reformulate in an alphabetical order and complete the list of abbreviation and acronyms; 2. Improve the project site map presentation. Delineate the specific project site in the map; 3. In Section 1.2.1, in order to keep the relevance of the project with the Objective (i) of the ITTA 2016; 4. In Section 1.3.1, add more information on the geographic location and the project site which include

a short description on area’s major physical features and ecological characteristic; 5. In Section 1.3.2, explain more specifically the social, cultural, economic and environmental aspects

of the target area. Explain the land ownership and local-tree based farmers; 6. In Section 1.4, rephrase or delete the expected outcomes (d) to avoid misunderstanding its context

within the overall project interventions. Keep the formulation of this Section in consistency with the indicators in the logical framework matrix;

7. Reformulate Section 2.1.1 in accordance with the ITTO manual for project formulation; 8. In Section 2.1.2, add a paragraph on the undertaken participatory approach on analysing the

stakeholders; 9. In section 2.1.3, add statements that discuss the encountered problems within the context of SFM.

Reformulate the problem tree in accordance with the ITTO manual, and add the objectives tree; 10. In Section 2.1.4, reformulate the Logical Framework Matrix (LFM) and use SMART indicators; 11. In Section 2.2.1, rephrase the development objective. Change the word ‘uplift’ with ‘contribute’; 12. In Section 3.1, in relation with the project Activity 2.3, add the established plantation as one of the

project’s outputs; List the project’s activities in this section; 13. Enhance the elaboration of Section 3.2 with paragraph(s) on plantation establishment; 14. Reformulate the budget arrangement to include the master budget table. Calculate and present in

the appropriate tables the budget item for ITTO project monitoring and administration. List the capital items purchase by ITTO budget. Clarify the use of the international travel costs by specifying intended conferences/seminars to attend. Include budget for financial auditing. Clarify the cost of land;

15. Enhance the elaboration of Section 3.5. Assumption must be elaborated based on the key

assumption identified in the LFM. Sustainability after project completion must be clearly explained;

-39-

Page 45: ITTO · 2015. 10. 6. · international tropical timber organization . itto. project proposal . title: genetic conservation, utilization and management of gall rust resistant strains

16. In Section 4.1.1, referring to the cover-page of the proposal, explain the listed collaborating agencies

and their roles in the project implementation; 17. In Section 4.1.3, include donor, NGO and industry representatives; and 18. Include an Annex that shows the overall assessment and specific recommendations of the

Forty-ninth Expert Panel and respective modifications in tabular form. Modifications should also be highlighted (bold and underline) in the text.

C) Conclusion

Category 2: The Panel concluded that the proposal requires essential modifications and will be returned to the proponent. The Panel will need to assess the revised proposal before it can commend it to the Committee.

-40-

Page 46: ITTO · 2015. 10. 6. · international tropical timber organization . itto. project proposal . title: genetic conservation, utilization and management of gall rust resistant strains

ITTO Comment Response

Overall Assessment The Panel acknowledged the relevance of the proposal to the Philippines and the importance to promote biotechnology to hasten tree improvement to meet the increasing demand for wood and its derived products. The panel also noted that the project proposal aims to conserve the disease resistant plus trees of P. falcataria. However, reformulation and clearer explanations to several aspects of the proposal are indispensable to undertake in order to understand the links between clones production, established plantation and end-use of the wood.The Panel also noticed information lacking on state-of-science to combat Gall Rust, land status for plantation, ownership of plantation and its beneficiaries. The Panel requested a supporting letter from the collaborating agency, CIRAD, to be included in the revision of the project proposal.

As for the comment on lacking of science to combat gall rust, we included in the list of activities the screening and formulation of biocontrol to combat gall rust; this was considered in the problem tree, objective tree, LFM, workplan, and budget. The letter of support from CIRAD was obtained to be submitted as attachment to this revision.

B) Specific Recommendations 1. Reformulate in an alphabetical order and

complete the list of abbreviation and acronyms;

We have arranged the acronyms in alphabetical order and included those that were introduced in this revision

2. Improve the project site map presentation. Delineate the specific project site in the map;

We improved the map by highlighting the project site and added some information on the location of target beneficiaries of the project

In Section 1.2.1, in order to keep the relevance of the project with the Objective (i) of the ITTA 2016;

4. In Section 1.3.1, add more information on the geographic location and the project site which include a short description on area’s major physical features and ecological characteristic;

Short description of the physical features and ecological characteristics have been added and highlighted in page 2

5. In Section 1.3.2, explain more specifically the social, cultural, economic and environmental aspects of the target area. Explain the land ownership and local-tree based farmers;

The social, cultural, economic and environmental aspects of the target area were expounded as indicated in page 2. Land ownership and local-tree based farmers were also described as highlighted in page 3

6. In Section 1.4, rephrase or delete the expected outcomes (d) to avoid misunderstanding its context within the overall project interventions. Keep the formulation of this Section in consistency with the indicators in the logical framework matrix;

We deleted this outcome and revised Section 1.4 according to the ITTO Proposal Preparation manual

7. Reformulate Section 2.1.1 in accordance with the ITTO manual for project formulation;

Revision is highlighted in page 4

8. In Section 2.1.2, add a paragraph on the undertaken participatory approach on analysing the stakeholders;

Paragraph on participatory approach in stakeholder analysis is highlighted in page 4-5 and made additional enhancement on the stakeholder analysis

9. In section 2.1.3, add statements that discuss the encountered problems within the context of SFM. Reformulate the problem tree in accordance with the ITTO manual, and add the objectives tree;

Encountered problems in the context of SFM was answered in detail in page 8-10 as part of the problem analysis section

-41-

Page 47: ITTO · 2015. 10. 6. · international tropical timber organization . itto. project proposal . title: genetic conservation, utilization and management of gall rust resistant strains

ITTO Comment Response 10. In Section 2.1.4, reformulate the Logical

Framework Matrix (LFM) and use SMART indicators;

Logical framework matrix was reformulated and used SMART indicators for each activity

11. In Section 2.2.1, rephrase the development objective. Change the word ‘uplift’ with ‘contribute’;

Development objective was rephrased based on the problem/objective tree

12. In Section 3.1, in relation with the project Activity 2.3, add the established plantation as one of the project’s outputs; List the project’s activities in this section;

We added the establishment of clonal plantation on the third year; activities were listed in page 16; consequences of this change was already considered in the workplan and budget

13. Enhance the elaboration of Section 3.2 with paragraph(s) on plantation establishment;

This was answered in pages 16-18

14. Reformulate the budget arrangement to include the master budget table. Calculate and present in the appropriate tables the budget item for ITTO project monitoring and administration. List the capital items purchase by ITTO budget. Clarify the use of the international travel costs by specifying intended conferences/seminars to attend. Include budget for financial auditing. Clarify the cost of land;

We reformulated the master budget table; The cost for ITTO project and monitoring was also included in the ITTO budget by component; International travel costs include possible conferences where research results of each project component can be presented; Budget for financial auditing is classified as Administrative/Financial Management Cost which is 15% of the total budget from ITTO, excluding monitoring and evaluation costs, Category 80-89. All foreign funds are automatically managed by the UPLB Foundation Inc, 15% is the cost of management fee, including the financial auditing. The cost of the land in this proposal was reflected as the value of its use, but it will be provided in kind by the executing agency.

15. Enhance the elaboration of Section 3.5. Assumption must be elaborated based on the key assumption identified in the LFM. Sustainability after project completion must be clearly explained;

Section 3.5 on assumptions, risks, and sustainability was enhanced in this section

16. In Section 4.1.1, referring to the cover-page of the proposal, explain the listed collaborating agencies and their roles in the project implementation;

Collaborating agencies and responsibilities were explained in detail in this section and revised in the table of stakeholders Table 1

17. In Section 4.1.3, include donor, NGO and industry representatives; and

Their involvement was indicated in this section

-42-

Page 48: ITTO · 2015. 10. 6. · international tropical timber organization . itto. project proposal . title: genetic conservation, utilization and management of gall rust resistant strains

ANNEX 5. Assessment by the Fiftieth Panel

A) Overall Assessment

The Panel considered the modifications contained in the revised proposal in response to the comments and recommendations made by the Forty-ninth Expert Panel. The Panel noted that the revised proposal satisfactorily addressed partly of the comments and recommendations. However, further improvement to the proposal is necessary in order to fully address the comments and recommendations.

The Panel opined that gender specific indicators must be highlighted in the revised proposal. The newly adopted ITTO SFM Guidelines may be referred. More information on the state of the art of the gall rust research outcomes must be added and referred in the project implementation.

The Panel also noted that the budget contribution of the Government of the Republic of the Philippines must be increased at the level of at least 10% of the total budget.

B) Specific Recommendations In order to fully comply with the conditions of Category 1, the proposal shall be revised taking into account the following:

1. Improve the presentation of the map of the project site. Include clear legends of the map; 2. In Section 3.1, improve the presentation of the Section with the correct numbering and

harmonize the listed activities with the activities presented in the master budget table (note: wording and number of activities are different) ;

3. In Section 3.4, amend the budget arrangement. Budget allocation for Activity 2.5 (clonal plantation establishment) is considered too expensive. Elaborate the details of the administrative cost (15%);

4. In Section 3.5, elaborate more about Risk; 5. In Section 4.1.2, explain how the project management team work and interact among

themselves; and 6. Include an Annex that shows the overall assessment and specific recommendations of the

50th Expert Panel and respective modifications in tabular form. Modifications should also be highlighted (bold and underlined) in the text.

C) Conclusion Category 1: The Panel concluded that the proposal could be commended to the Committee with incorporation of amendments.

-43-

Page 49: ITTO · 2015. 10. 6. · international tropical timber organization . itto. project proposal . title: genetic conservation, utilization and management of gall rust resistant strains

Overall Assessment

The Panel considered the modifications contained in the revised proposal in response to the comments and recommendations made by the Forty-ninth Expert Panel. The Panel noted that the revised proposal satisfactorily addressed partly of the comments and recommendations. However, further improvement to the proposal is necessary in order to fully address the comments and recommendations.

The Panel opined that gender specific indicators must be highlighted in the revised proposal. The newly adopted ITTO SFM Guidelines may be referred. More information on the state of the art of the gall rust research outcomes must be added and referred in the project implementation.

The Panel also noted that the budget contribution of the Government of the Republic of the Philippines must be increased at the level of at least 10% of the total budget

(All changes to address the comments of the 50th Panel of experts meeting were indicated in this revision in bold and underlined letters and highlighted in RED. Gender specific indicators were added in Section 4.1.1 Stakeholder mechanism. Results of new studies on gall rust were reviewed and included in the Section 3.2 implementation approaches and methods (Mychorrhizal/Rhizobium-F. moluccana association) By reviewing the contributions of the UPLB-CFNR and DENR-CARAGA, we added component which the two offices can provide to the program, hence increasing the previous contribution to at least 10%. Changes are included in the revised master budget

D) Specific Recommendations In order to fully comply with the conditions of Category 1, the proposal shall be revised taking into account the following:

Improve the presentation of the map of the project site. Include clear legends of the map;

We changed the map with more detailed and with clear legends in this revised proposal.

In Section 3.1, improve the presentation of the Section with the correct numbering and harmonize the listed activities with the activities presented in the master budget table (note: wording and number of activities are different) ;

We improved the numbering and wording of the activities in master budget as it appears in the list of activities in Section 3.1.1 and in the Gant chart

In Section 3.4, amend the budget arrangement. Budget allocation for Activity 2.5 (clonal plantation establishment) is considered too expensive. Elaborate the details of the administrative cost (15%); In Section 3.5, elaborate more about Risk;

We amended the budget. Budget allocation for activity 2.5 was reviewed and the changes was indicated in the revised master budget. The earlier revision was unintentionally computed for 3 years and was already corrected here. The details of the 15% to be collected by the UPLB Foundation, Inc., the institution that will manage the fund to be given by ITTO, was elaborated in the Memorandum of Agreement as attached in this revised proposal as supplementary materials, including their certificate of registration, organizational structure, and description of how the UPLBFI works. This was addressed in this section.

In Section 4.1.2, explain how the project management team work and interact among

We addressed this concern by presenting the structure of the Project management team

-44-

Page 50: ITTO · 2015. 10. 6. · international tropical timber organization . itto. project proposal . title: genetic conservation, utilization and management of gall rust resistant strains

themselves; and through a figure, which was included in this section, and short text describing their relationship as well as how the activities are interrelated were also explained in this section.

Include an Annex that shows the overall assessment and specific recommendations of the 50th Expert Panel and respective modifications in tabular form. Modifications should also be highlighted (bold and underlined) in the text.

Attached as Annex 5

-45-

Page 51: ITTO · 2015. 10. 6. · international tropical timber organization . itto. project proposal . title: genetic conservation, utilization and management of gall rust resistant strains

Annex 6. Curriculum Vitae of the Lead proponent/Program Coordinator

Lerma San Jose-Maldia Home address: 489 Brgy. San Miguel, Santo Tomas, Batangas 4234 E-mail address: [email protected] Contact Numbers: 043-3124452 (Home Phone); 09298101371 (Mobile) Educational (Tertiary) Background/Training - Doctor of Agricultural Science (2010); Master of Agricultural Science (2004). Nagoya University

Graduate School of Bioagricultural Sciences. Chikusa-ku, Nagoya City, Japan - Bachelor of Science in Forestry (1999). University of the Philippines Los Baños College of Forestry and

Natural Resources College, Laguna, Philippines

Field of Specialization/Research Interest Population Genetics, Phylogenetics and Phylogeography, Tree Genomics, Molecular Ecological Genetics, Evolutionary Biology, Conservation Biology, Forest Ecology Employment History and Community Services

• Assistant Professor 3, University of the Philippines Los Baños (Feb. 5, 2014-present) • Assistant Professorial Lecturer (Sept.2013-present). De La Salle University-Manila, College of

Science, Dept. of Biology • Postdoctoral Researcher (2010-2013). Forestry and Forest Products Research Institute, Dept. of

Forest Genetics, Tsukuba, Japan • Teaching Assistant (April 2007- March 2010). Nagoya University Graduate School of Bioagricultural

Sciences • Intern (Aug.- Oct. 2009). United Nations Center for Regional Development, Nagoya, Japan • University Extension Associate I (1999-2000).UPLBCFNR Training Center for Tropical Resources

and Ecosystems Sustainability On-going research project Project leader. Long term monitoring changes in plant community structure in the Mt Makiling Forest Reserve. 2015-2016. PhP 300,000 (6,500 USD) project from the UPB-Basic Program Fund Project leader. Molecular analysis of drought resistant fruits trees in Region 1. February 2015-Nov. 2015. PhP 648,000 (14,400 USD) collaborative project fund from the Don Mariano Marcos Memorial State University-Commission on Higher Education Representative research article in ISI Journal San Jose-Maldia, L., Uchida, K. and Tomaru, N. (2009) Mitochondrial DNA variation in natural populations of

Japanese larch (Larix kaempferi). Silvae Genetica 58:234-240 Megumi K. Kimura, Kentaro Uchiyama, Katsuhiro Nakao, Yoshinari Moriguchi, Lerma Jose-Maldia and

Yoshihiko Tsumura. 2014.. Evidence for cryptic northern refugia at last glacial period and anagenetic divergence in southernmost population of Cryptomeria japonica. Annals of Botany.

Membership and Involvement in Professional/Scientific Associations Japan Forestry Society; Ecology Society of Japan; International Association of Ecology; Society of Filipino Foresters; Gamma Sigma Delta Honor Society of Agriculture; Philippines Association of Japanese Ministry of Education Scholars; Nagoya University Alumni Association-Philippines; University of the Philippines Zeta Beta Rho Honor Fraternity-Mussaenda Honor Society Alumni Association Recipient of Japan Ministry of Education, Culture, Sports, Science and Technology Scholarship for Doctoral Degree (2006-2010) Matsushita Electrical Ind. - Panasonic Scholarship Inc. Japan for Masteral Degree (2001-2004) Philippine Agricultural Resources Research Foundation, Inc.-Philippine Council for Agriculture, Aquatic, and Natural Resources Research and Development, Full Scholarship and Thesis Grant (1998-1999) Other Personal Information Date of Birth: March 28, 1977 Place of Birth: Sta. Maria, Laguna, Philippines Nationality: Filipino

-46-

Page 52: ITTO · 2015. 10. 6. · international tropical timber organization . itto. project proposal . title: genetic conservation, utilization and management of gall rust resistant strains

Supplementary Material 1. Organizational Structure of the UPLB Foundation, Inc.

Board of Trustees: Name Profession Position Years on the

board Dr. Fernando C. Sanchez, Jr.

UPLB Chancellor President and Chairman Over 20 years

Prof. Rex V. Demafelis Vice Chancellor for Administration

Vice Chairman Over 20 years

Dr. Jose V. Camacho Jr. Dean, Graduate School Member Over 20 years Dr. Josefina T. Dizon Dean, College of Public

Affairs and Development Member Over 20 years

Dr. Felino P. Lansigan Dean, College of Arts and Sciences

Member Over 20 years

Dr. Domingo E. Angeles Dean, College of Agriculture Member Over 20 years Dr. Juan M. Pulhin Dean, College of Forestry

and Natural Resources Member Over 20 years

Dr. Isabelita M. Pabuayon Dean, College of Economics and Management

Corporate Secretary Over 20 years

Dr. Casiano S. Abrigo, Jr Executive Director, UPLBFI Executive Director Over 20 years Dr. Ernesto P. Lozada Treasurer, UPLBFI Treasurer Over 20 years Atty. Chito C. Avecilla Legal Counsel, UPLBFI Legal Counsel Over 20 years Atty. Filemon D. Nolasco Legal Counsel, UPLBFI Legal Counsel Over 20 years

-47-

Page 53: ITTO · 2015. 10. 6. · international tropical timber organization . itto. project proposal . title: genetic conservation, utilization and management of gall rust resistant strains

Supplementary Material 2.1-2.4. Memorandum of Agreement between UPLB and the UPLB Foundation, Inc., explaining the details of the role of the UPLB FI to handle the fund administration and the details of 15% Project Administrative Cost being charged to the project fund. (Please see separate files in pdf)

Supplementary Material 3. By-Laws of the UPLB Foundation, Inc. (Please see separate files in pdf).

-48-

Page 54: ITTO · 2015. 10. 6. · international tropical timber organization . itto. project proposal . title: genetic conservation, utilization and management of gall rust resistant strains
Page 55: ITTO · 2015. 10. 6. · international tropical timber organization . itto. project proposal . title: genetic conservation, utilization and management of gall rust resistant strains
Page 56: ITTO · 2015. 10. 6. · international tropical timber organization . itto. project proposal . title: genetic conservation, utilization and management of gall rust resistant strains
Page 57: ITTO · 2015. 10. 6. · international tropical timber organization . itto. project proposal . title: genetic conservation, utilization and management of gall rust resistant strains
Page 58: ITTO · 2015. 10. 6. · international tropical timber organization . itto. project proposal . title: genetic conservation, utilization and management of gall rust resistant strains
Page 59: ITTO · 2015. 10. 6. · international tropical timber organization . itto. project proposal . title: genetic conservation, utilization and management of gall rust resistant strains
Page 60: ITTO · 2015. 10. 6. · international tropical timber organization . itto. project proposal . title: genetic conservation, utilization and management of gall rust resistant strains
Page 61: ITTO · 2015. 10. 6. · international tropical timber organization . itto. project proposal . title: genetic conservation, utilization and management of gall rust resistant strains
Page 62: ITTO · 2015. 10. 6. · international tropical timber organization . itto. project proposal . title: genetic conservation, utilization and management of gall rust resistant strains
Page 63: ITTO · 2015. 10. 6. · international tropical timber organization . itto. project proposal . title: genetic conservation, utilization and management of gall rust resistant strains
Page 64: ITTO · 2015. 10. 6. · international tropical timber organization . itto. project proposal . title: genetic conservation, utilization and management of gall rust resistant strains
Page 65: ITTO · 2015. 10. 6. · international tropical timber organization . itto. project proposal . title: genetic conservation, utilization and management of gall rust resistant strains
Page 66: ITTO · 2015. 10. 6. · international tropical timber organization . itto. project proposal . title: genetic conservation, utilization and management of gall rust resistant strains
Page 67: ITTO · 2015. 10. 6. · international tropical timber organization . itto. project proposal . title: genetic conservation, utilization and management of gall rust resistant strains
Page 68: ITTO · 2015. 10. 6. · international tropical timber organization . itto. project proposal . title: genetic conservation, utilization and management of gall rust resistant strains
Page 69: ITTO · 2015. 10. 6. · international tropical timber organization . itto. project proposal . title: genetic conservation, utilization and management of gall rust resistant strains
Page 70: ITTO · 2015. 10. 6. · international tropical timber organization . itto. project proposal . title: genetic conservation, utilization and management of gall rust resistant strains
Page 71: ITTO · 2015. 10. 6. · international tropical timber organization . itto. project proposal . title: genetic conservation, utilization and management of gall rust resistant strains
Page 72: ITTO · 2015. 10. 6. · international tropical timber organization . itto. project proposal . title: genetic conservation, utilization and management of gall rust resistant strains
Page 73: ITTO · 2015. 10. 6. · international tropical timber organization . itto. project proposal . title: genetic conservation, utilization and management of gall rust resistant strains
Page 74: ITTO · 2015. 10. 6. · international tropical timber organization . itto. project proposal . title: genetic conservation, utilization and management of gall rust resistant strains
Page 75: ITTO · 2015. 10. 6. · international tropical timber organization . itto. project proposal . title: genetic conservation, utilization and management of gall rust resistant strains