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Volume 24 December 15, 2013 3775
2013 ASCB Annual Meeting abstractsThe American Society for Cell BiologyThe American Society for Cell Biology, Bethesda, MD 20814
The abstracts of the 2013 American Society for Cell Biology (ASCB) Annual Meeting are attached to this article as searchable PDF files. To view the files, click on the “Abstracts” link in the content box in the middle column of the HTML version of this article.
The ASCB and the Editor-in-Chief of Molecular Biology of the Cell (MBoC) have decided to present the Annual Meeting abstracts in this manner so that they will be clearly associated with the ASCB’s basic research journal and so that they can be properly archived. The abstracts of the 2010, 2011, and 2012 ASCB Annual Meetings were published shortly after the meetings (American Society for Cell Biology, 2010, 2011a, 2012). The abstracts of the 2005–2009 ASCB Annual Meetings were published retroactively (American Society for Cell Biology, 2011b).
Historically, abstracts of each ASCB Annual Meeting were published in a printed supplement to MBoC. (PDF files of the supplements for 1992–1996 were recently made available online; see Table 1.) That practice was discontinued in 2005, and abstracts were instead made available on the ASCB’s website. Thus the histori-cal link between the abstracts and the journal became tenuous, although authors were encouraged to cite ASCB Annual Meeting abstracts as having appeared in a supplement to MBoC. The associa-tion between the Annual Meeting abstracts and MBoC has been restored by making the abstracts available in the online journal.
Archiving of meeting abstracts is important because they are of-ten cited in the scientific literature. They need to be permanently available. Although the ASCB is committed to maintaining abstracts
from its Annual Meetings since 2003 on its website, it is prudent to preserve such documents in multiple locations. The Annual Meeting abstracts will now be available on the MBoC website at HighWire Press and in downstream repositories such as PubMed Central as well as on the ASCB website.
All abstracts submitted for ASCB Annual Meetings are screened by the abstract programming committee to ensure that they meet minimum submission standards. However, they are not peer-reviewed.
Abstracts are citable. Citation styles vary by journal, but one way in which 2013 ASCB Annual Meeting abstracts may be cited is as follows:
Smith AB, Jones CD (2013). Abstract title. Mol. Biol. Cell 24, xx (abstract #)
DOI: 10.1091/mbc.E13-10-0584Address correspondence to: American Society for Cell Biology ([email protected]).© 2013 The American Society for Cell Biology. Two months after publication this article is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).“ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society of Cell Biology.
Year URL
1992 www.molbiolcell.org/content/3/supplement.toc
1993 www.molbiolcell.org/content/4/supplement.toc
1994 www.molbiolcell.org/content/5/supplement.toc
1995 www.molbiolcell.org/content/6/supplement.toc
1996 www.molbiolcell.org/content/7/supplement.toc
TABLE 1: URLs for abstract supplement tables of contents.
REFERENCESAmerican Society for Cell Biology (2010). 2010 ASCB Annual Meeting
abstracts. Mol Biol Cell 21, 4299.American Society for Cell Biology (2011a). 2011 ASCB Annual Meeting
abstracts. Mol Biol Cell 22, 4705.American Society for Cell Biology (2011b). 2005–2009 ASCB Annual Meet-
ing abstracts. Mol Biol Cell 22, 3555.American Society for Cell Biology (2012). 2012 ASCB Annual Meeting
abstracts. Mol Biol Cell 23, 4663.
MBoC | ASCB ANNUAL MEETING ABSTRACTS
ORAL PRESENTATIONS
165 Optical thickness and optical volume measurement of live beating cardiac myocytes K. Creath1,2,3, G. Goldstein1,2; 14D Technology Corp, Tucson, AZ, 2University of Arizona, Tucson, AZ, 3Optineering, Tucson, AZ
This paper describes recent research related to optical thickness and optical volume measurement using a dynamic quantitative phase imaging microscope. Results are presented of quantitative measurements of optical volume changes in beating rat cardiac myocytes before and during treatment with IPHC (isoproterenol hydrochloride). Full phase and topographic data are obtained at 15 frames/sec. Live cells were prepared and grown on #1 coverslips or coated slides. Cells were placed into a Bioptechs FCS3 perfusion chamber and kept at 37°C. Videos of cell optical thickness topography were generated and processed to obtain relative optical volume. These results show dramatically increased rate and strength of contractions with application of IPHC.
The microscope developed for these studies provides instantaneous measurements of dynamic motions of live cells without labels or contrast agents. It utilizes a pixelated wire grid polarizer mask in front of the camera sensor that enables simultaneous measurement of multiple interference patterns. Optical path difference (OPD), optical thickness (OT) and optical volume (OV) data are obtained from phase images. Simulated DIC (gradient), simulated dark field (gradient magnitude) images and 4D typographic movies can easily be derived from these images.
Cell Shaping ePoster Talks
ASCB 2013 Annual MeetingNew Orleans, LA
ORAL PRESENTATIONS
SUNDAY, DECEMBER 15
POSTER PRESENTATIONS
262 Optical thickness and optical volume measurement of live beating cardiac myocytes K. Creath1,2,3, G. Goldstein1,2; 14D Technology Corp, Tucson, AZ, 2University of Arizona, Tucson, AZ, 3Optineering, Tucson, AZ
This paper describes recent research related to optical thickness and optical volume measurement using a dynamic quantitative phase imaging microscope. Results are presented of quantitative measurements of optical volume changes in beating rat cardiac myocytes before and during treatment with IPHC (isoproterenol hydrochloride). Full phase and topographic data are obtained at 15 frames/sec. Live cells were prepared and grown on #1 coverslips or coated slides. Cells were placed into a Bioptechs FCS3 perfusion chamber and kept at 37°C. Videos of cell optical thickness topography were generated and processed to obtain relative optical volume. These results show dramatically increased rate and strength of contractions with application of IPHC.
The microscope developed for these studies provides instantaneous measurements of dynamic motions of live cells without labels or contrast agents. It utilizes a pixelated wire grid polarizer mask in front of the camera sensor that enables simultaneous measurement of multiple interference patterns. Optical path difference (OPD), optical thickness (OT) and optical volume (OV) data are obtained from phase images. Simulated DIC (gradient), simulated dark field (gradient magnitude) images and 4D typographic movies can easily be derived from these images.
ASCB 2013 Annual MeetingNew Orleans, LA
POSTER PRESENTATIONS
SUNDAY, DECEMBER 15
Imaging Technologies, Single Molecule Imaging, and Super-resolution
Abstract “Label-free” quantitative measurements of optical volume changes in beating rat cardiac myocytes are presented before and during treatment with IPHC (isoproterenol hydrochloride). This newly developed microscope provides instantaneous measurements of dynamic motions of live cells via a pixelated wire grid polarizer mask in front of the camera sensor. Videos of cell optical thickness topography were generated from quantitative phase data and processed to obtain relative optical volume. Live cells were prepared and grown on #1 coverslips or coated slides. Cells were placed into a Bioptechs FCS3 perfusion chamber and kept at 37°C. These results show dramatically increased rate and strength of contractions with application of IPHC.
!
! Objectives
! 50X (NA 0.8) ! 20X (NA 0.45)
! 1X or 2.25X FOV lenses ! Source wavelength: 511 or 660 nm ! Fast data acquisition – no scanning ! Real-time processing – 15 fps ! Vibration insensitive
Microscope Specifications
References [1] Creath, K., and Goldstein, G., "Dynamic quantitative phase imaging for biological objects using a pixelated phase mask," Biomedical Optics Express 3(11), 2866-2880 (2012). [2] Creath, K., and Goldstein, G., "Processing and improvements in dynamic quantitative phase microscope," Proc. SPIE 8589, 85891A (2013). [3] Goldstein, G., and Creath, K., "Dynamic four-dimensional microscope system with automated background leveling," Proc. SPIE 8493, 84930N (2012). [4] Creath, K., "Dynamic quantitative phase images of pond life, insect wings, and in vitro cell cultures," Proc. SPIE 7782, 77820B (2010).
Optical Thickness and Optical Volume Measurement of Live Beating Cardiac Myocytes!
Katherine Creath and Goldie Goldstein 4D Technology Corporation, Tucson, Arizona and
College of Optical Sciences, The University of Arizona
Acknowledgements We thank our team at the The University of Arizona led by Drs. Andy Rouse and Ron Lynch. This work has been partially supported by the National Institutes of Health (NIH/NCRR 1R43RR028170-01, 2R44RR028170-02, and NIH/NIGMS 8 R44 GM103406-03)."
December 2013!
CONTACT: Kathy Creath <[email protected]>, or Goldie Goldstein <[email protected]>
(Top) Microscope schematic for epi-illumination with a Linnik interference objective. (Bottom) Transparent samples in liquid are imaged under a coverslip on a reflective surface. This system measures relative integrated optical thickness (OT) [or optical path difference (OPD)]. OT is related to both physical thickness and index of refraction variations.
Optical Layout
Effects of Treatment on Cell Morphology
Optical Volume Before & After Treatment
Relative optical volume over time series of 200 datasets. The same area of cells (above) is compared before and after pushing IPHC. Note changes in both strength of contractions and speed of contractions. These are indicative of changes in the force of the contractions.
Rat cardiac myocyte cells cultured on #1 coverslip. Measured in a perfusion chamber at 37°C with 40X at 660nm. Optical thickness and 3D topographic maps of two areas. Data series taken at 15 fps for analysis below.
Before IHPC After IHPC
Optical thickness and 3D topographic maps of embryonic rat cardiac myocyte cells cultured on a #1 coverslip. Measured in a perfusion chamber at 37°C with 40X at 660nm. Data series taken at 15 fps.
Rat Cardiac Myocytes
Comparison of Optical Volumes
Optical volumes of 2 areas of above culture sampled at 15 fps (lower right “Cursor 0” and “Cursor 1”). Notice variation in strength of contraction in different areas of the culture (upper right). (lower left) Histogram of optical thickness values.
Like a color camera that sees in phase (or polarization), phase shifts are obtained to simultaneously determine brightfield, phase contrast, dark field and DIC images. Fast data acquisition using short exposure times with a pixelated phase mask enables measurement of moving samples without blurring or scanning.
Enabling Technology
Applications ! Morphological Studies ! Mechanistic Studies ! Tissue Dynamics ! Quantify & Track Cellular Motion ! Process Monitoring ! Quantify Cellular Changes with Treatment
Rat cardiac myocyte cells cultured on #1 coverslip. Measured in a perfusion chamber at 37°C with 40X at 660nm. Optical thickness maps of two cultures imaged at 20X (top) and 45X (bottom). Data series taken at 15 fps.
Rat Cardiac Myocytes
