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Kassidy Klink
Peak Environmental
Site analysis
Molecular Biological Tools (MBTs) and Chemical
Analytical
Microbial Source Tracking
In-situ Microcosms and Bioreactors
Passive sampling tool for microbes
Collects active microbes
Integrated sample vs. “snapshot”
Analyzed using molecular biological tools, analytical
chemistry, and stable isotope analysis
Molecular Biological Tools
o PFLA: Quantify entire population
o CENSUS (qPCR): Quantify specific populations
o DGGE
o Stable Isotope Probing (SIP): Prove biodegradation
Chemical Analysis
o Compound specific isotope analysis (CSIA)
o Dissolved Inorganic Carbon (DIC)
o Contaminant Concentrations
Simultaneous analysis of different targeted microbes
Quantification of all targeted microbes with single
analysis
Quick TAT of between 10 to 14 days
Determine if known degraders of a COC are present
Evaluate MNA versus enhanced bioremediation
Compare effectiveness of amendments designed to
stimulate bioremediation
Prove that bioremediation of a specific compound is
occurring
Reductive dechlorination
MBTs / types of analysis
Deployable biotraps
Insitu microcosms
Deployable bioreactors
