2009 research priorities

Cleavage degeneracy

- partially randomized libraries in vitro Cre, Mso, mCre, mMso (Hui)

- single site scans by recombination induction in vivo (Umut, Hui)

- end capture from genomic DNA/Solexa sequence (Stefan)

- insertion site tagging/sequencing (Stefan w/H-P’s lab,Byoung).

New designs for disease gene/Anopheles targets (Umut)

More active/’up’ mutant versions of Mso (Hui)

Benchmarking of native/engineered HEG’s

- expression/stability/toxicity as f(activity)/in vivo sites above (Stefan)