2 Manage Burewala Strain of Cotton Virus PC-I Page(6-8)

8/8/2019 2 Manage Burewala Strain of Cotton Virus PC-I Page(6-8)

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Mlnaging Bure\\ala Strain of Cotton Mrus IBSCV)

rrNrsrRy o!' !'ooD, AGRlcuLl uRE AND LIVESTOCK

COVERNMENT OF PAKISTAN

ISLANIABAD



a

PC-1

Managing Burewala Strain of CottonVirus (BSCV)

" MINISTRY OF FOOD. AGRICULIUBE4IIO LTYESTOC(



6'

PC-IPART'A'

| !4U!qE-!!grBqlEEL

,) !ss!!qN

" Mana$lng Burewala Strah ofcofton vhus (ASCV) "

2. 4!IE98UIES!ES!QTSIE!q(2&

Midislty of Food, Ae.i.ul'ue @d Ljvcsto.l (MINFAL)

ii) Ex.ecuTIoNThis prcjet will be run b, MINFAL in couaboratioh wilh Palisld Cental

Corton Connitle (PCCC) md Prcvincial Asicultural D€Parhents. ftedctsils aE s follov,

. Fcdedl Cov.mncnt

a) Minisr.y of F@d, Asicuhuie & Liv€stck (MNFA,,b) cehral cofon Rese&h Innnoie (ccRJ), Molknc) Centnl cofton Res.qch Itrsdute (ccRI), s'kand

d) Nnci€d lnsdute for As.i.oltue & aiology OUAB), Iaislsb.de) National Inslilutc for Biot€clsology & Cenetic Engiheerihs (NIBGE), Faisalabad

. CovcrnF.ntofth(Prnjnb

a) Direcror Cotlor Resdch lnstitule (CRI). Ayub Asricllilral Rcsedch lnstilule,

b) Dir€dor Ccnet.l Asricullute (Exrensioh & Adlpive Rese&ch). Punjob Lahore

. Cov.rnF€nt ofSindh

a) Cotton Botsnist, Agriculture Resc.dh lnstitur., Tandojan

. CovcrnneniofBrlo.htul,n

a) Dnecto. C€ffil Alt.olldrc Exletsjo,, DepnnDent of Asiculture. Qudta

. cdtrftn.ntoaNwFP

a) Dterj.l Oltcer Arliculttrc. ,^Bnculture ExleNion Depaimcnr. D I Khd



,iD ORGANIZATIONS INVOLVgD

,) Mitisrry of Foo<j, Agriculture dd tirest6k {MlN[At')i) gakistd Ceffal conon conbi(d (Pccc)

c) ?alGtm Atonic Enersy Connission (PAEC)

r Gov.rnnent oflne Plrjtr'

r, D,r.ror 6odsl Agnrullure ( Rcsech,

b) DrBlor uenersr Agnlultu.(Frrensron& Aqapr've Ke\e!(nt

Dirertor Ceneql Act.ultu6 {R*dch)

GorcrnDebr or Bdochittrn

Dnector Geflqal As.iculrute (Exicnsion)

L

a) DirecIe Cet..,l. Agricqltute Pxlension

TIMD RT,OIJIRED FOR COMPIf,TION OT PRO''ACT

4l Mon6s (Dcftdbd I,2Oo2loJue30 2006)

4. ,) PI-AN PRovlSIoN:

i) If t!. projert B in.lud.d iD lhe cldtrr'iYcv6'r

pht''p&ift

r'rutl

alloc.tion.

Not ,gpli.nble

ll not lncltrdeit in the clr.nt pl.n, how ls il no{ prcpos'rt b rP

aic.nn"arr"O O'r*nt"ts.ctor;t I'ljuslmctrl' in 'lr"'rido' otn'r

r.sou.cB bry bc indicllcd)

,,)



tabl.'2 At., Poduclbn dnd Yttld ot CoAo

Coron failure or poor yieldi tue onen oulcone of biod. nd 3btotic std$cxteriebed by plels duing fi€ir liac sp Anons rh€ bi6tic facbrq conon plont

hs b€en under diecr threal ofclcuv tud Rhitcny, Nnich is vecrdr ofclcov



,o

Coitotr Le,aCurl Virus -MullM Sh.in

Cotto. Leafcurl Virus(cLcuv) Muila Strain appearcd in 1933 and its inlestationircresed every y.ar in the subscquent !.riod. lne calaslrophic efi€cis of CLcuVarGct on collon wcre lithesFd during 1992 93 .nd in tlE subsequ€nr yeds. In 1993-

94. aboul 0.39 hillion hectaes w€r. badly dadagcd resulting about rNo niUion balesloss in poduclion due ro CLCuv. ln econonic ((m. tne coontry fiad sqffercd

"l6s

ofaboul T,6 million blles, which cosrs lo the tuneofRs.7l billionsincc 1988 duerollE i.,ee.rion oI CLCuv.

A th@ yed auon leaf curl virus prcj€cr Ms stancd i. 1995. Asie DelelopmenrBa.I piovided a los of US$ l0 nillion aor this pojecl. The Pimiog edDevelopmenl Division nade a blck pbvisioh of auhds ro Minktry of Food,

As.ic,llud & Ln'esl*t Tne DDWP jb Minbrry of Food, A$culrurc & l,iveslck

appoved 3l Dojecls for vdious inslitrtions a CCRI, Mulrani NIAB, NIBCE,CEMa, AARI, Faisalab.d dd CCRI, Sar{r.nd, CRS Tmdoian. Cenhl CoiionResedch l.stitule. Mulan\ scientists successlully idenlified (lrce Esislote€notyp€s (LRA 5166, CPl52 md Ced€x) to CLCV. Aner idendllcatio. ol rh€segenotypcs, sientists rcr€ able lo .el4 . Itge nuDbcr rf v,rjeties !! CCRI.Multd, NIAB, and AARI, Faisalabad. Thcse veiedes hclped Paknran lo lide ov.rlh. CLCV icnace md put Pakishrs cotion producdon Drcsraftmc in iotward sce.

Burwrla Sir{in olCorlon Virus iBSC\')

Rec€ntly, corton leafcurl litus h"s asain emerged as a key dne6e in thc povince ofrbe Pubjnb

'ngcneal and Bwwala aea in

'aniculd.Th€ re-.mersence oa vias

annobly called .s Burewla Skain oiConon viNs (8SCV) ha dqsercus venioned @uld develop inlo a seriou p@blem. h is suspsted 1hal pevious vids st6in ham aled fld resis|ad v&ieries ae showiog syntloms ofCLClv nr tie wnob @tlonb€lt of Punjab. Evcn $e parcnt n.terial (LRA-5166. CP-15/2, and Ceder) u$d to

deve,op cotl@ Je,t cur, vjtu rsjsiarr vdjeties has aho b€en found suscertible inBue*al. t€rilory Th. infesladon oi CLCIV discse has crossed lhe boudaries oflhe Punjab fld enletd ihto the neiehbouing povioc.s.

Thc delelopned oa bisrdt vdie(i6 to BSCV would require scrcening of lvailable

EedplM showi,E rcsistat e b BSCV' lDderemc oI CLCIV esi$ancc fhn c,4rroferr dd *ild species into G ,ia,r,' tra.sfdcnce of isect resnthc€ 0R)eenes ibto cohnercial cLcuv rcsisldt vdietics, ropularion dyraoics of 4.,'6trr"ra.i (veclor of CLCuv), nonitoling oi iheclicide rcsisroc. ro , r.6ac'! sweyingand NessFe ot loss due b CLCIV. nulirional $udi* / chenical basis ofresisradcc, srodies on cdppins sysred, chetui..l \Eed cmllol. attcio! usc offenili4rs, imgalion ed pler densny, dev€lothcnl ofm rcl ru.agemcnr shlcgya.d ls{_y dhsenination of ihfom.tion to lhe l'n6s dd srakeloldeG thoughhuuimcdir inresrared approaci Developnent of virus iesnrer vaiietics withdesirsblc fibie cbdacteristics tbrough .on conventional nethods such s! Acnelic



€nginering, indrced hulalions md relaled teclDologics *itl cone ioNard to fisht

ll IB b€r €poned tlrrt .apid .4ohbirald .rle mong vjtuses j. c,usihe r$jslecbtak doM in nosl ol tne blerml and r.sistanr vanefts at Bureqala. In rhis endles3

.fte bet*e, pjal od rjds, tlf is ned |o cbdscreiiE esistdce breakios srnitual noleculd level ed rer&ch ior new v&idts This i.cludes clonin! of sel€Gdroh *. scquenlingsd Inlc!nq( saly,r of Bscv su,in.ln rh(r.pe'r,a srclli'"DNA called DNAp is cssentally requiFd ior causing disese syhptons and is al$Gsciatql with BSCV vriat€, Moreover, rsing DNA in(erferehce (DNAi)tchnology for developi4 bo.d sp4trud rcsisrane againsl AsCv will strngdenpace of res€dch lo €volv. BSCV nsistam ldieties.

Therefod, theic is d usenr .eed ihat intesrared and interdisciDlinary resetuch b{

underh*€n for d€rclopnfll of ASCV .esisrat vricries rd .ulruEl ]lffieeoerlstmlesies on wir loori.s bcaoF the probleh eel dangcous dinensio! hurlins lhr

Cohb.rins oa lhis disease will ensure slability ahd increase in national codobproducion, *bich is cnviegcd in ih. five-yeo plan io meet local frill consunrflionfld cxpon r€quiemeft. Cod forbid, couniry *ill beai a loss oaRs.l0 billion iflheeis reduclion of frrce rnillior bales in collon pbduclion, lf such situarion continues,counlry vill b€ in a qtaslroFhic situalion. Thc tequiene.l ofDediunr lo.s, lons fldexrF long staple cotoh is I2.3,2.5 dd 0.2 frillion 6alcs per ycar Especrjrely.

Slrpl4ii.@qut@erls otE .otoD hy2005.2010

0.2

5. Nf,I,ATIONSHIP OF THE PROJECT WITTI OBJECTTVES OI SEC'TOR:Indicrt. rhc.ontribution ofp.oiect. ouantilicd it possibl.. io t.rf,€k itr th€ fiy.r€rr pbn. .hd nane of orhd prci.cls (*heth.r srr.rion.d or undsrpnparartonl which Bould ford b.rts oaan intesrared wiihin secror:

Tre pojecl is ih @.sonancc *nh ii€ objccrive ofrhd &ncllior S€to. ro imp.ovelhc incone of the fancr. Tne estihalcd conshptio. of 6w co(on of tcxrile i.dusryof Pakisrd is ll niilion b.les by rb. yea.2005. Tbe ncw rhrear of CLCV {BSCV)



tuy becone a Dajor liFitation to achieve the itrgcl dd lo meel local and exponaquiFhenis. The developne.t of CLCV (BSCV) tcsisram veielies md adolrionofndasenc.l pr.clices to mininize the los in yield would esnh in increae in s.edafton yi€ld Per $il lmd 4a.

6. CAPIIAL COST OT TTIE PROJECT

Sumnary ol the PtoJect "Man.sing orBur*.|. Str.in Cotton VirE (BSCV)"

b) Foreisn Exchdg€

\'r\l NirNil

2003

2003-2005

2005-

22922 l2a9l 62.t32o 120 3.160 1 205 6,445

2 310 2UO 2 530 2.210 9.390

0 450 16 225 3.025

0451 3796 6823 5058 21.12A

0 350 r 900 I 900 4,325

s|l{DH

2.142 3.630

2 025 2025 t-rT".*

1.025 1.025 I 025 0.525

COORDINATIOI{ UNIT o.745 I 671 1752 6.012

TOTAI 23,792, 64.94' 34.O73 26,89 t49.045

7 A]VNUAL RDCUR'TING LVEND'TU'E AI TER COMII ITIOI:



8. OBJECTIVNS OT TTD PROJECT PREFERABLA IN QUANTITATTVETTRMS:

CENTRAI COTTOII RESEARCH INSIITUTE, MUITAiI

. Sc@ning ol arailable ceftpldb for evalualion of suc€ ot resisbncc lo cotlon

r Developme of hiSh yielding col{on lqa curl virus esislant codmeEial

. Delelopfrcnl of comnercisl corton bybrids resist|m ro CLCV.

. Sludi€s to i denti fy the mecbanish of ihhclilhnce a I CLC V relista.e.

. Developne.l ofinre4pecific hybids reshthl ro new slr.i.s olleafcun vitu.t ldendfi@tion ed lransicr of Bl Sene i. @dmercial varieties.

. Developoent of t{hniques for Esenedion of corlon plut frcm cokerl I2. 315nd local BenoltTes.

. tbe"emrMic

studies of vdi€des developed bI the brcedeB wilh sp*idrcfercnce b CLCUV.

. studi.s for €Jliciol r oflcnilia wirh speci.l Efereoce ro cl-cuv.

. Role oftlml de.-.y in nininizing los4 qused bycoiton lealcurl vitus.

. Weds ad tl€i. effeds o,.otlo..op iD CICDV e,!iroD€,r.

. w.ter mtusgehent srrategies for pofir2blc @(on produclion in couoh l€alcul

. Studies of coppins syslen qirh dst€d lo CLCUV diseasc

. ld.ntilicariohof suspededhostplm(sofCLCuVii major@tidnsdwiasdc6.

. Sufrey and assessnent ol losses in corton producrion dle ro CLCV dis*.

. virus tdnsnission fon colon pldr lo vrious olhcr nost plbrJ ed vise vesa.

. Populalion drnmic ofr?zisia,zDa., dnrios pre. and posl, co(on fdon.

. Role oi allcmste host plants for supival of a rab,.i md ns pddiloids

. Mohitorhs ol isectici'le resislane b A @ba.i

. Scrtenine of semplasn fo! tolennce/suceprible b , kl,ci

. LvestaalioD or the nadrc !.d kven$ of nuti€nr dnord€n and rhcn relationshipvirh lh€ ihciden€e or CLCUV i. vdious .e!o clihatic zoncs oflne couon belt.

r Srudies o, cldical bsis ol resisltuce asainsr CLCuv in lhc susccflible dd

. FieJd expetD.r,s iD s'udy |he iileDclion ol nacro, and ni.io, nudenls invanous combinatons in rclevsce ro colton ploductivily dd incidcnce ofCLCuV

. A technic.l bochud on nutri€nl dnordeS sod thci symproms io cotton for Eady

r Studi€s oh $e €fl4t of CLCUV di*ae on libre quality. The omherct l vrieti* ffd pmmising sldi6 oa rle P,rjsb ,nd Sirdb $ll be

tesled et Cotloh Resrdch Stalion, D.l. Kha (NVFP) ro screen out rheit lotemce



The poducrion of basic &d pd bsic @nonsed oI culrivd besr suit€{l ro D.t.Khe ehyironnent tud turther ils dulripli.ation.To en@unge fmc* to arcw noe co on in $e new tues avrilsbte aorcuhivalion b pbvidihgqulity

sed to fmers in D.l. Ki&.To'nctac

yield per u.it land arca rhioush provision ofquality seed md CLCVdisease frc inD.l. Khan.Disenination of infomation ro rhe tmes ltuoush multinedia im€grar€dlpptonch lhrclgh famers lield shool (FFS) ih $e conoD b€ll.

MrctEAR IXSTTTUTEFAISALAAAD

Derelopfrenr or .ouon v&ierFc tud eempt^nr hav'ng h'gh )retd po,entEl.$ideradaprdbilir!. CLCuvre!tuLe dd d.nEble qu.nry , hdro. r.rr(ic

rsdpt"lehSlh 29.5 lo 10.5 mm witb microhaire value (4.0 -4.5 rs/inch), for lieituiiliation i. coton b@dine Dogrdne of the counlry md roanongsl collon ercqeB abou advaned producrion tccbnolosy.CFaion of senetic vaiabi|ny for CLcuV dsistancc lnroush lne usc or inducedntrlllions.nd e@ning of geneic veiabilily (i.duced. local, and.xotic) assinsl

r,s ot CICDV resi$Dr .owr ls) io collor beediry prosrame ro deveto! hishyieldi4 tud virus resnrat vari€tics having good fibcr qudity.Sa&li6 Io derhid oode of i.t€.ittrc. fo. resistee.

ITATIOIIAI T{SITUTE FOR BIOT€CHIIOIOGY & GENETTC ETIGINEERIIIGFAISALABAD

Mol*llar characterialion of Buewla md otner potchriol Esisrtuce bicakingslr.i.s of cottoh leaf cud virus.

Develolhent of RN?\i constructs for broad s'.cltuo rcsisnnc€ agaimt Ccnini-

Dekloprnenl of ANA, consh.rs for cono! leal c!., djsea* bsed on a DNAelellile called DNAp thrl is $sedially dtuir€d for rhe developnent of @0on

Trdsfomarion of cohsrNcts in Dodel and conon plds &d evatuad.n oflansseni. plants for virus resistan@.

scken'ns ofdinerent colon.n.cFs agdinsr rhc Bucqla \ituhApplietion of DNA togerpri.rins lftlmology b nnd DNA potynoQhisnJmong fie conon spccier forsen.denrilcarion snd senomersppingApplication of Bio-infofta1i6 lfthnolosy to seaich foi DN?\ sequ.nces ofdifledl sress Elaled sen s ed lrifrer d6ieningDNA sy.lbe$s.lslation ofdilfereft genes coofetriog Bislmce .gainsl the diese.Iffcacy oI Meler Assistsd sele.rio, {MAS) j" come(ial cotroD srains

FOA AGaICUITURE At{D BtotoGY (Nraa),



COTIOI{ RESEARCE IN'IrIUIE, FAISAT.ABAO

CENTRAL COTTON RESEARCH IIISTITUTE SAKRAND

To qeate Ce.clic sbieid ?sainst n€* Btrrew,la CLCuv sbsin by pyrdidihe

tdedincarion ot Be.e conlirarion ror Cl-Cuv resi3tancc in available marerirl

IncoqD.ar'o. ofCLCuv resisianl genes b good ljbrc quahy and high yielding

To altenpl ttu3fer ofcLclv resistol genc frcm D€si (c' ,rrozzl) cotto. iltoAmaicm 1C. ,tr uu) cotlon }igh tietdins ed good tbre quak/

SNq of CLCV incidena at R€semh Stalioos nd tameB field.

Scr€enins of aaailable filial gencration halrial ed advanced lines lgainstBurclala CLCY tîon.Prcvnion of rsoues to diueenr Cdtron Research Stations i. Punjab lo catry our

elllctoriyraneral introveme.t plosmhmc.To determin€ dte condbudon of norphological trdirs in planr rcsislancc rsainn

To nrd out the dosl ef€ctive dd efe insecticides for $e contol of wbneny,

To {ork oui the €iltrI ol inisation, fedilizr dd planl density on rhe

developndnt of qhn€fl y popularion.

To srudy fie host pref€ienco of whilefly in clops, vcgerables and wecds.

Recod diseasc incidenG, sevenry &d variely infoftation uhd€r natural

rmdidons tron difleEnr Co on growing Esiohs of tE ?unjab.

DcteDinalion of dhde scverny &d vaiely beh3viou ttrough sra0ins uodcr

Colleclioh of collon leaf cuil virus (CLCUV) infecled sdples frch diltae.lgeosEphiclcarun\in<oronsro*rn8trgionolrhcFunjabMoldular analys;s ofthe coll€cbd smples in lhe Laboralory lhrough PCR and

biolo8ial malysis in Orcsdt rooh.

AGRICL"TURE (EXTEI{$OII & ADAPTIVE RESEARCN' PUNJAB LAHORE

Th. o'snll objectile of rho pDjccr is to incFa* couo. produclivity lh@uBh

mmas€mcnl ofclcv. Efons will be made ro educlte and l.air rhe flmeB wirh

regrd 16 lll lossibb llffires fd eiTeclivc and cconoFic conrol of C'LCV,wbich has becohe a kev diease ofcolton cro!.

Sufr€y ol coftI c6p in Sindi and Balochisld.

S€r€eninsoaexolic lines orSindh dd Punjab v:licties as,inst the vftsDev.lopmnt of CLCV Esisl6i nacrial,Detemining sucki.s pcsts, €specially whiteny as vector ror lhe virus.



kol.tion aod inoculido. tecbniques lor lhc vitus.

lolcofnurhrion Mo'drgation

In rhedeeeropnrcnr or the \'d.D(ect of qeds on co(lon cmp in CLCV enlironnrent.

Up'sDd alio. of ex ist ing faci Ii ties at CCRI, Sakrand.

colTol{ RESEARCH STATION, TA!{DO]AM

Testine of cofion srainylin€v v4icli$ for rcsist nt to CLCV ir CCRI, Mulr,iCRS, Sini*al dd NTBCE hielabad in lujab where CLCV dis@se is pevaiting.Hybridiation of comeEial @d ewly d.veLlcd hish )ieldbs laieries wif)CLaV Esisrer varietier

Teslins ot Filial sen€rarions for €valuilins agai.sr CLCV dkqs ar diffrEntlocatio.s in Sindh and Puslb, (CCRI,

Mrll.!.CRS,

Sahnld,NIBGE.

Fai*labad).Resisra.l mr€lial will b€ resrcd in breedins t.jal.Dclelop reh,ologJ, for dMgeoenr of whirefly i, coloh.ld.n(i6utid of$hnefly speci6, oc€uing in co{onScreen out .ono. vtuierics eiislsl (o *hiefl, infe*arim.Sludt de horplDlogical charicld ol plat rspodibtc tor tdis(se (owhitenyrcLCV

Fiq+Nr biolqical @lroi fte1hod lor ndasemcdr ofwhnely in colton.

surer of @non Ehwine de6 of Siod[ € s tlE gevaleNc of litu diseae ed@lleclio. oI disesed smpler.

lde,tncrlionoa vitus

dise fbd collcted s@plcs rlhueh NARCIVIDCESc@ning of gBlplah of cous a8ainsr 'itu discae,ldentificalion of virus on otler Khrif crops.

De jncaftn of axemale lDs pldts.

Codhercial veieries a.d pbnising srrains of rbe Punjab ad Sindh {ill betesled .t Agricuh\€ Delelophal ln$nute, Xlrepur. lJsla MulDrtndd lo scFenoul llEir resistmce to CICV dise&e snd their sraFabiliry under i6e

To dourage f@ers to g.ow mon couon in lt€ new d@s for culiivrtioo by

lroviding seed to fmers.To increde yield per uil led &ea thmugh quainy sced_lh€ pojed will sivd a basic knowlcdac lo researcbrs, cxrension worters dd

AGRICULTURE EXTTNSIOI{ DEPARTMEIIT, QUETTA, AALOCHISTAN



AGRICULTURE EXTETISION OEPARTMEI{' NWFP

. Comherifll Eielier &d pbhising sr.ains ol rhe Punjab ed Sindh will bebs{ed in De& rmail Kh3n and Bdo d* 10 screen out $eir r€sisrode roCLCV dise3e ed rhen dapl?bilny under the €nvtomenr.

. To €ncourage fmcrs to grow nore colton in the new are6 for culrivarion byproviding sdd lo f@€rs.

. To ihcE se yield per unit l?nd aea tl&usl quahy seed.

. The projed witl cive a ldiic knowledg. 1o Eseeh.6, exrension workeG ddfanners in parliculd.

FEDERAL COORDINATION UI{IT

! AdSdcnls of budsd prcvisions. Coodinationmd facilita{io.of acliviliesof tbepej€ct.



CHICKIDBY.

A

l4 ]s!.f rl[t"hrnn',d ft{.ft Gill

Dicror,Ccrtr.l Collon Res€arcb

r,,r!h& \" /tr,

s

,lr )-41l' ]'-l)r- M rl,,'n'n.d H.nif

Asricultural Developdcnt Con'nnssiorer

r- llr l> -' >-



PART'B'

PROJECT DESCRIPTION AND FINANCE-

LOCATION OF ttIE PROJECT: GIVD NAME oF PLTICES ANDADMTNISTRATIVE DISTRICTS IN \'dICII THE I{ESEARCII CDNTERWILL BESELECTEN

'l herefot, the exisling nelwork of asricuhunl r€scmh al iederal and provincisl level

lnd exrension will be involved i. ihh prcposed rrcject. The Fsearch a.d extension

facililicsat dif,erentoryanizarions.r€ as follows:

10. EXISTINC FACILITTES: give nforn,tirn nbotrt tlc orsdnialions doinasinilar res.nr{ork!t pr6ot. indicate their srrll, budgct f.,r the lasr live yc.rsadd rh.ir .(tpil in tnns of numD.r oI rldid e{., menrjon it rber. ir luyo.grnizrliob lo enstrre coordinrrion of Fo.k done by diller€nt rescarclr

All the f€deral lod provnrcial dsearcb nNdutions havc NeU.eslablished rcscarclr

laboratones, ereen houe, expedmenral liux and larm dachinery to conducr

€sd.dr D, lle Dullidi$jpjln.ry sd Dulrj-djDc$ioDal asFcts of $e prcject The

inslnudons have sufficiehr number of experienced scienlists a.d supporliDg peônhel

to achiele tbe objectives or lhe pojst undt vaious agro-clinaric Esions of rbe

provinces wilhin the siipulated period Under thc piese.l set up, connodny specific

FsaEh ins ules/depannenls have bccn undenaking rcsarch on the de!€lopncli ofvaieties md impoved production lechnology !scka!!

a) CLCUV PROJECTS 1995 - 1997

,The CLCV poject was innialed with llte US$ l0 niuion assistance from AriatrD€vclopmerr Banl i. 1995. A Felerol Coodinaridn Udt qas established nr Miisryof Food. AeicultuE & Livestock wnh ll sub-proFcts in rarious rese!rch

orgdialions at CCRI, MultsD; CCRI, sakaDd, NIAB. NIBCE, Iaislabad, ARI.

Iaisalabad, CDMB, Lanore, CRS. Tandojm. 'fhe achierenehts made by wiousi.slitutons dc descibed as below:

C€nhal Cotton Research lnstitute, Mullan

Keepins in !iew, the severily ofthe problem, aM lo sustain the cconony of lhe counry,Covmenr of P.iistan siclion€d projects wo h tu.23.9284 nrillion to onduct resenrch

oD vdious dpecls of CLCUV dheasc. 'l\e unrnine rescarch ellorls of $e ccRl, Mulianscientists r*ult€d ir rhe evolurion of Cl-CuV EsisraDi varieiies. Thc llsritute is the piohe€r



a.-insttution to cvolv. ev€r insl CLCIV reristari v!.icry CIM Il00 rhlr p,v.t way ror lhe

dev€lopMt ol succelsile CLC{V Fsistan \aielis in fic &uD1ry. aential Couon ResocbIns !1e, Mult& developed six CLCnv csislanr leicries tidrdesn.bF fibre char&terisrics

Ibese veicties occupy aboul 55 perc.d aFa in dE Pujab proln\.e. Due (o lb. Nlt rli@of rher CLCV iesistdnt v&ieties. $. Punjab provincc harvested 8.8 nrillion bales duri.g1999-2000 con$ted 10 6.5 niLlis bales d$ilE r99l-9,1. ln addiiion 1D lhis, epidcniolosical

srudies wero undertalen to detemine lbe tturbnsior ofCl,CV and its bohaviourdl patteF

in ihe 6nd bel. S$dies or n61 pl! Esisldce cspecidly {hireny we.e uddend}s to

dininjze the i.frslation $rcugh shircny. Inlesrated prcduction tcchnology was devetoped

10 mininie the ldsses ol $e discase.

co(on L6 Ctrl Virus fi€dsr.nl vai6ries Oevoloped by cCRl, Murl,n

GOT

t%)

ctM-t 100 1996 330 29A

1936 34.0 933

1993 276

t993 J62 21.4

ctM462 2000 392 245 930

2002 39.3 296 952

Developoent ol CLcv Esislmt vadelies resullcd at avedge incesc in production ol1.2nillion balet worlh Rs. l2 billion pft amud.

In addirion, 7 cultiles ol @non have bccn Eleded aner hybridizatioa with lN(aris tavingspecilic trai$ al NIAB tud oth€r Provihciai ed feder.l Collon lrstilules. Of thesc cullivajNIAB-Kdisle is Ihe most siediftrmt mveing abo( 2{% dd usder .o(on in lbjab

Nucleer ln3ritute fo. Adrlculluro A Bioloov (NlABl. FaisElabad

Unds th€ prcjrct t{o CLCV Esistdi slro! veie{es (NIAB 98 ard NIAB 99) Nqecdntribured in the Narioml Coo.dinaled Yield Triah orgeized by PCCC durins 2000.

SeteBl Nt61 liretslqins &e .ls in the pjt€line, shich h.le sh.M e\cellent p.rfoinecewnh rcspecr ro CLCV r€sis&ne ed yjeld l)ot€nl'al and are expared to be iicluded in huhibarion rrinls in rh. coDinB )e&s.

Th€ nrin targer ol tlF projecl was 1o Ecnrrale aeflDplasm. reiabiliiy for the isolalion ofCLCV r6istat Inener yielding vdeties/gemrypes of coron with tlE dev.lopnent olresisrmt vuieties since 1996lbe dada8e rlue to lear curr vius has bee. reduced in drc

coutry ro a tignili@t level-



aNational In3litoro for Biotechnology & Genetic Engineering {NIBGE),

NIBGE is a ledortl preDier esqrch ins ule of tr\Ec Nnh a nandare to apply modch andimovalive lsbniques in a8riculluro. NIBCE has.lonc pioneering work in undoseDdi.s lhe

complex naturc of couon loaf cul vis discase (CLcuD) by rnolecutar chftercriaiioa ofqusali ve sgenh lhat would esuh ih devhins ne w stmle gies lor contbl of lh e d iscde.

l- tlirus Charu.lerintian:Anoog sev€n sub pojecr of Vnobej,, tie con|.ibution ofNItsCE wcre exeptid.auy highleEl dd €alrmelr hrlpfnl in Dde6imdibg md nm,geD.nt or CLcuD. fu dira* was

found to be dscialed wifi a amplex of dhlinor oonop ile begodoviruses (DNA A), a

heovirus like nolecule olled DNA I and a DNA sarellile called DNA 0 rh n.slenrial for

the d.velopneft oldiseas synplons. DNA p dcpends on DNA A lnr encapsid,tion edta.sntsion lul unlike bilelile begofloviruses shaie no sequence hofolosy wirh DNA A.Inoculation of DNA A ofone $e disinct conon begoDoviruses wnh DNA P is suJticienl to

ca6e dis6e symplons in collon. Despile highly divcise natun .rcollon besoDoviNssseia&d wilh the disqs, a sinsle sD€cies of DNA ll is associaled wnl fie dis€de. These

esults shows llld DNA p is lhc najor playq in evolurion offie dise,se thar can capture

div€rse DNA A that ae able lo replicate in couon. Tlus rhe con*ned .arue of couon B

Fovides a nolel leg€l for ensin€erins rcsistoce aeainsr cotton leafcun disease.

2- Da.lophent d Tethniq @:NIDCE develop.d bauery of tesls (wbitefly hanrnrissio., Nlultiplex PCR, Dot blot Nay,eleclron nicroscopy fld in{ectioN clone) for rhe detection of vtus. l bis is the o.ly ce rewheE all lhe iecbniqus N ftlly lunclio.al md conlinuourly applied. Tnes seflices are

provided to all lrderal a"d lrcvincial cenlr€s.

J- Epi.leh io los! Stu.l es :

lor the nanasenrent ol tjie disease, epjdenriologl studies lvere taken u! on sciendfic brsis.

AU the bilosical ed mol4dd 6says were pcitomEd art dlere(e h.sls oa rhe liss vereidenti lied. Tnse stud ies conlriburred d necdy in rbe ma.aeenrenr o a r he d i scase

The enablishnert ofDNA linserp hlins lecbnology in Paki5ra. is dorb.r nalhaik of thisplojecl. Tbe studi€s rcrc conducted o! all fie pre md post CLCV era varielies ofcouon.These slndies wre helpful 10 EebuE dile.sily .mong our larielies. These fi.din8s w€requit€ rlamins dd indicaled very Mrorv sen€tic base. Our nessagc to couoh breeden was

loud and cld. Rece.t emergence olftsistance brcakinA Burewala virusofCLCV h obviououtcooe of jg.o.ing this scientific dat!. Molecul.r ndke6 for CLCV and olhcr r6ils mnear conplelion. Th€ delay is nalurdl !s popnlation developDcnt hkes r-J years.

5 Derelophehl dcereticdll! lihgine.rins ynus Resitut CaI.h:The objeiive 16ro!sh hiel$ anbitiuN atrd chdq bDt fiis sas mado possibl€ ar NIBGE.Coiton lrasfomflion is fully eslablishdd md CM pl tuls conrinuo usly beinsdeveloped. Tnisis a nev technolocy md erclusively i. lhe enrol of Mulli Narioml compflies (MNcs).Despire inl€rnalioml bui€rs, exlemive work Esulied in derelopment of two tnnssenic tinesof S 12 and sdl lh6 ot CDker 112 {Modcl Coton pl.n9. t, rce bse.t rcsistad lnles lR,



aFB-901 , IR-C|M113 ord )R-C|M-q], aa.l o"e vitns rcsistntl soxtu NIBCE-I nrc beitE.wlt tq{ n co6r.inhut ta.ikry ii t'. na ho6e nrd nt tt. 6"arch Jield serffii AM.orcn pldts *nn ldied gene conslrlct3 are i| th. pe line

Cotto. Rosearch lnsiilrt6. F.isrlabad

CrCV wd n€ver a trobled in PaList{ befoie 1988-S9. h raas \oliced i. 1968. Enuged !sdpoblen in 1989-90.Ils sprcad b*me ponoun ed ed danas,ins d{rins t99?-91. sFe.dis incidenrrlly coEelared with rhe disserindlio! of S 12. county bec@e fon net erro@r

oi rnw co[on lo ner inpodei ilie catastrollic efllcl of cl-cv looded ovd co on fion1992-9] onward. CLCV m checked wilh lhe release of rcsnranr vrieties i e. ClMl I10,

co"{ 448. CtM446. ClM44], CIM 432. rH-900, FH-901, rH-6]4, FVH-51, BII-118.

tlowever, lesietant of dFR'dieties

\ate bcs bro[d d@n and usent elions de rcquned

F d.\elop.l.\ F hr$' vnel".r r'.d .u.d),he !"r{ oi F urd(n. e o| t c\ b qell $undc.sredins of whiteny lopulalioh dy'mics. Tie Preliols Ct,cv Fojec( res rcd is drc

Flease of rsisltut v&elies such * FH-900 and FII-9oi, which fted nnhediate

'eprrener', s bod minellc.uvFabdnn rcB cl.v a.!

VAdETIES RELEASEO BY OIFFERCT{T BESEARCh STAIIONS Of CRI, FAISATASAD

1. FH 6U2 FH 900

3. FH-901

5.

6.

CR9, MurtartvNH329

t\,rNH554

i\,lNH552

1996

2000

2000

45

43

40

50

363n7538.8

41.0

42540.0

285?9426.7

28.0

29.!27.1

1998

2000

2000

FVH 53 1998

BH-118

36.3 285

39.0 280000

cenlral Cotlon Rosearch lnditulo. Sakrand

Sludi.s vft cdn€d olt for one sctson and as sucl nolhi.S solid could be concilded. olen

tl\en the inirial dtsrPadons vqe qlile dcoula8itsi) Sone natul eredies hive betd ide'rtiicd as !t!,tnr V, b.letnocctut sp,.

Crryrdru sp. elc. whicb can bc uscd in pes contol as biohgisL ae.q1,

ii) Ferrilid.l.vels of 100 ts N tud 50 Pper hd have beeo panially estabthhed

iiil Thc sosinsdare has been rol.d shieh nishl benetr Io the optimuD. Ar sa*rdd ltisnay be rroD l0rh Mly ro lorh May.



acotion R€search St tion, Tandoiam

In Sindh rhe Cl-cuv dis6e ws obscfled on one srowcts' field in Talura UbaN in 196.

Ai€trards the die& ilciden€ is spEadins slorvly & eraduall) lnd ha been t@ded nqr

SalmdTalula Howve!; lhe

inlmilyof disede remined bq dd widonl.najor innucnce

on Foducdon Th€ posilio intmsily of CLCuv in sindh ftonr 1999 lo 2002 is 8iv€n 6

THEYEARAND TALUXA-WISE INTENSITY OF CLCV IN SINOTi

Y..Fwbe %age of Inr€nsiry

1999 i 2ooo 2001 20002

1-2%

2.

1-2

2-3%

3. 5-6

56

10'11

10-11

453-5

23

4-534

59

5.9

9.3

88

5.3

5,20 al0

100

4512-15

1.00 ?.9

5.1-3

t-3 4-5

riorl. Ourg khaf200r cdr'orsalra'ard Sn.Lro'oralklarof Sanshar dislict srth int€nsily ranqinq trcm 01-1.55 Sniary, n Hyderabad dislrct t was

Eponed rrcm Haa taruka bul infesralioi was in iG@s and iegligLbe



aIt is lieed ltul du€ to exislenl incidehce ol CLcv disle in Sinnb, i( nray i'rcftbl if noderail 6eeh votk on rhis pmblem is nor rat<en in hdd d.d erctved couon lejetics Esislat10 CICV diFas dd lhproved Crop MaMsenenr TeclDiqucs.

L&Ung to rbe sw€rily of CLCV dieae a sub-project endlcd "Mdasehent ofLcal Culviru Disesa TlTouel Evoltrdon ofResislor Coton V&ieries by Bio kchnotogicat Bieedine

Techniques (Sindh ohponent)" vd appmved for teiod of 24 honths

Ttu total cosr ofltc pojet {c as sdsi.

2.266

a 423

2 266

4,245 o.423

PRESENT STATUS OF RESEARCH ON BUREWALA VIRUS

SyDprons ol CLC! on viit Esitlml vdi€ties scre observed in Seplenber 2001 by lhcscientisls of CCRI. Mulla! tud daned teslins oflirus resisianr varieties agairst rhh vitusthouCh gani4 ed whitefly lmsnission al CCRI, Multd. lh€ iisl Nort lrodisincRnsrdrhaknal*a5 plhEd in rhe tor .po' red\ rbr ruah.r \renr

'eFollowire joiol .ftons of federal ad proviociaL reiedch insli(urions s€ h.ise sdde 1o srud]turlher CLCV problen io lhe Bue{ala der.

AII vdielal tials teins cooducted by Fed€dl or prolirciqL RsaKA lss(ilures bare conllmedb@kdown of r€sistde asainsl @tlon leaf curl vids in Buresab aDd vehdi dea. lncidence

ofdiee on di|fer€Dl vdi€ties od advactd lines varicd froD 20 lo 70%.

A tobl of2938 lin€s including setmplad aod adlaoc€d lines fron CCRI, Multe; CRl.Faisalabad; CRS, Multm &d NIBCE, Ioisalabad weE llflted at Cottoh Eseeh sblio.,Veh&i lor $reaing agaitut Buewala virus. ADong these 229, which have not, shownsymplom s ill a€ under obseFation.

VIRUS DATAOFGENETIC SIOCK PLANTEDAT COTTON RESEARCH

S]ATION, VEHARI.DATE OF OATA RECORDING 28.09.2002

212



aSnRey o f aU .oton-soNing d islriqs of P dnj ab has bccn catricd o ut lt was concl uded tron

sweys that Buewala stdin hs spsd tpto Veh.ri bul maior ddot gtuwjng disLicls

includins Mulun. Lodttfl, Bahawalpur, Rahin YBr (nd, Dera Gbazi Khe m'r Rardpur

e no1 afi6led bY this slrain.

Moleitrtd cidsieriation olcottoo leafcurl vi.us tts lEe, irl,ited al NIBGD Inl4tedsmples lere colleded Buerala &d vehari &ea Tohl DNA ale{iral DNA have been

plrifid dnd virus @mp!@l! Itve been olomd. l,iljsl Psnlt sh.wed 3% difietunces in the

DNA bela component *er€ det€cEd fronr edlier cloned DNA beta.

Moleculd nethods have ats bed usd 1o id€nily altenaic hosls ol cotton leaf cul virus in

Bdewal, od Vel*t d€a- One srsldl hosl denlified froh Bueqala @a t chilies, w\ich

d wdely qrc@ bl fmers on small tueo ll $d" IoJnd lhdl "mpl€s ol.hil,es .ollsred

t/od l"jr r;s $er infpded wirh conon leal .url \itu $hrle sple..o'ltuLeJ rbm orhe6

wE infered dilh olha gediniviruses

Eleclbn nicro3copic siudies nave detecled the presence of scniniviru p?nicles ih iniected

sntles. gow€lcr, no orh€r vns p.nicl€ olher than Seninivnus {as detccled

P€lirnindt wrc bei4camed oul by CCRI., Mullah, NIBGE, CRI &NIAB need inpurs to

sl'eh8th.n rc$dch a.dvnios. Conce{ed efons and conli.uily of rc*ei lindida is

expecled to Esuh in elTecliae control of the dhease lfnot contollcd, il is fcated lhat lhe

dieN will dr.sticall) reduced yierd and qualilv of cotlon in Punjab llE a.e!d ot J3

million bales equiFd by the yed 2005 will refranr elu$ve dtoan

b)CURRENT RESEARCH FACIILIIES AVAILABLE

central conon Research Insftute, (ccRl) Mulian

C€ntal Co(on Re*&h lNtitute. Multh w.s eslablished at Mulh in 1970 The inslitut€

has th€ ehfte inars(tu(@ idcludids dapower to.Dnd&l DuJlif,ce|ed reedch on cotton

prodrdion. u h4 sllicie laboratory spa*, .! experinenbl fh, waler $d Porerfaciliiies io @y olt Poposcn es€e.b l*o$lane

The iosailut€ is o"drcli,C fud4e,,tl ed applied Esesrct in alllhe disciplircs of cotton

pldt @nelyi crtogcnelics, Plant Dreeding and Genelics. Agononv, Phvsioloev/Chenistv

bnloioloer'. Pl€,t P.rbology, libE Teclrnologt, Slatistics and Trdsfet of Tdbnoloev Edchsecrion is haded by . Se or Scienlist, who h6 recciled training abroad h 1he respedile

disciplim, M6r of the stali in lhe inslitute hd ah experience oi about l0 yea6 in $etr

i€sp;cdle lietds. The instil$e h6 38 re*sch scieDtists and vell-equipped labodtories of

The vdiou rsearch Isbo@tories arc equimcd witb adrbc€d appa61u and otlrer facihies

b sork otr the sDecG oacolion dises and ils conrcl neasurcs. Thc@ is no olftr inslitute

doias sidile rcaeNb work. Mo@vc. CCRI, Multe is a mullidisiplinary lnsifure. ln



alens ol equipnents. lhe lnstiiule has hduclively Coupled Plasma (tCP), Aton c AbsorptionSpetopholoneler. Eleclro-llllanltladoh Appai.lus. Lrv-Spe.trophoronetet, A{tosaricA@ Metei lhobqeler, Pcsse Bonb Apparatus, Micsscope, PCR nacnnE. ELISA plate

ieader, cel Docunentation syslehj Cameh Micbscopc, water Distillery machine, -85.C

UllE low FEZi. Miclo Ceolrifuge nachine. cool incubator, I-lnind An Flow C.binet,Uitra Ce ilug€, Htbridiedon Ove[ Autoclales, Frce2cr (-20'C), lcc-nacker. watei bath.Fume hood, Cene Pulsd, Elcctrcphotesis syslen, D-code SysteD, cool Orbirll Sbaker,

Biolytic, Cnene-Dd. Cold lhcubation, Inscci Grov,lh Climbers. lNecticides for Insetr.eisia!c., Berl€as tunrcls, lots Tosq, lntraslruclLre ior lnsect Rcari.s, ,\ulomatic mne

collecting nachihe, Elalric Balahces, Micro Applicaros. Blending Appahrus dd Ilol

Nuclear Institut€ fo.Agriculture & Biolosy {NIAB), Fai3alebad

NI B is *eU equipped for various aspec$ of g.cullunl ard Biological Escarch. An

experiDenidl tam of about 200 acrcs is altached wnl lhe ft(itur€ for fieldwo andproduclion ofpre-basic and basic seds. Tbelnsr'luteIEs o!€r90scienrisk oniis srafl,l0 ofwbich ar bevingPh.D dcgree, Th. Laboratoriesofthe Instilure arc quiie well equipt d wirhusul itutrun.nls such as Diciosope, ceokifuge, ulrracchl.ifuges, UV, aronJc absorprionspecroneler, ClC, HILC, Preparadve IIPLC, electophorcsis equipnent, hdiodcdvnycolnt€s dd nonilo*, faciliries for PCR and IiFLP analysis. fibro sraph and orherhsku'N61s ro slud] rtt qlali$ ol colton ibre.

NIAB is well int€erared inlo the asdcuhure systeo ofde country ard has vely sood Iinrasesvith thc depanment of Asricuhuie (Fed€@l a(I l,!.!ircid) nnd al$ collabtrdns wnhpublidprivare or8dianio$ for'th€ production ofqualilt seed ol collon and olner crops by

prcvidios lh€ brede.s seed lo d€velop c€rlified seed.

& G€netic Ensinee.ing (NlBGEl,

The Nalioml lnslitute lor Bio€chnoiogy aDd Genelic !.sineering (NiBGE),lraisalabad h6a {ell-established iniiastrucrute and highly hined manpdwer to 0ary oul research woik,NIBCE has all lhe b$ic facihres requned b can! oul nictubiological. molecultr bioloeiGled ultm{Jwluci (ttussisior elelJor Di.roscop)) Nork. rh. basic racilit'res includeincubato6. autoclav*, wall (3 - l0 L) tud larec (150l) Iemrentod, bigh sleed cenlrifuges

(Becknan). ullreentituges (Beclmtu & llitachi), nicofuges, chnmarogdpbic colum.s,contolled rempeFt@ waler baths. ldinar itc! hods. luG h@ds, spectrophob$eles,prepmlive gel elrctFphoEsh, pube neu sel elecroplDresis, lder sceni$ densiloDeler,

UVaVIS photo'docndentation sysleh, probo counlcrj liquid scinlillalio. couorer (l,KB),theinal cycles (Perkin EIhe. Hybaid), probe counrer, lntud sci.liuation counter (t-KBl.COr incubalor, liquid nilogen cell sroraBe systeD (Taylocwhanon) and relaled cquipDent5,

DNA sequencins appadtus (Bio-Rad & LKB), biolhlic Bun (Bio-tud), DNA syntbesizer(Phtrm&ia), FILC (Pham&ia) dd microscopes ircludiis Uv hicroscopd, double b€mspe.rbpholometer, aromic absorplion specrrcplDroneler {ith furruce, elemenlal oalyser



ellj d FIIR'@hiRe.

h dddilion, tuI! eqlippcd Franl Broyih roons, two s"l! in cold

mom. gla$house dd e ."inral houe is avdilable. Rccemlt. NIBG€ b.s acquiEd

Transhissioi Electron Microscope (TEM) ed DNA Seq!en.e., sbicn nill b. rery useful

fo. rh€ prcj4t. NIBCE tas also its l.csl !.ea nel*.!k or conpulem.

Cottdn Resoarch lnslit|lle, Faisalabad .nd ils research gtations at lllultan,vehari, Rahihyfi Khan, thawalpu., sahikal, Khanpur, Khanowal, Thatbcurnani, Haroonabad, Kot Chutt , Jhang, Sarqodha, Piplrn.nd Ralwind.

Entonological Research Station,Faisalabdd

vjrclog Seclio", Faielabad.

Tddare 30 colron vdietics have been elolved by various iesearci sations, which co.dbnledincredine and stabilizing couon yield in FuDjab Enroarologr section ed PId vnology

*crion hav€ oolrihutcd lreNnd.usly in €srcb on yhitcnr.' and CI-CV iesFectitely.

Corto. Rcearch rnsrirute, Faisalabad and otber se.lions ofAARI, r_aisbbad hav. necessary

nrfGtruclure nlcluding mnpoEr lo codet nultif.c€(cd reeonh s co{ton lhdlclionuhder va ous geosraphical condilions in lhe corto. bell oalne Punj,b lt lEs suilicient

la!,mlorj spacc. elp€inenFl f.rhs. wals and lower supplies lo cary out th. proposdd

Fsea(h progrdmc. Moslofthe slallin rbc lffliluteh.s an cxnerictrceofs6outli-3s years

in llEir rcspectile lields. Tn€ Inslirutc hrs nroE llEn 27 rese,rch scientisrs rvho are well

expenekd ib inen ireld. SDhe.qriPhent and opcrational iunds are requi€d to accclerare

pace oftresent cs*rch soik

Asdculture (Exteneion & Adap{ivo Research), Lahot6, Pujab

Extension slalt !p ro the Union Council h a$ilabld to imFleDent the projed nr Ue cotto.

c.nt al cotton Research InBlilute, Sakrand

I]roush, there are everal organizalions doine similar research on corlon leaf.un virus lile,NIBCE, NIA, PARC e,tc. bd lh€se aE auldple .mp rcsearch oieanranons Norkins on

diferehi crcps snd it is $e only laristan C€.tnl Couon Conhitlee, r'nich is so'6 K!Dnltldhciplined Fsrarch orga.i4tion in the coumr), dealingwilbo.ly cotlon crop.T hus, it

heorcs th€ $lc rerpoNililig ol Colro. Res*ch lnsliiutes to deal wilh sound-foolinssagaiNt Corton ldf Crl Vitus di*se in Pakisran

Bec.use ofde diilirences in as!.-e6 clinratic condi(i6n! ol Pnnjab nd Sindn pn,vnres. itb€cones inlpc.atile to lat€ prercntive De.surc. aeand dE evailing lnus accordinstolhecondiiions oiSindh drd Baloclisld prolinces.

Th. C,ental Cortoo Res4rch lneftute sakad belongs ro rhe Pakistdr Cenbal Colton

CoiN e iPCCC), Mininry ol Food, Agricultuie end Livesiocr, Governmenl of Parist&,



aTn€ In$nuF s6 rstablish€d in Nov.nber 1916 as nDro_crop rnulti_ disciPlinary resdch

m.rirde uidr rheobjec|ivslo Ectle,olon relcred mobrn olsridl'!'oL'{e Thel6lirrrc

" do'nq boLn ,DDned ffd lm(Lnenrdl re*tr.\ .omb'd ns rhe .ndcdvod ' uf AFrononv.

BRd'fu. td,;;lo$. Pajrolocv " Ph) no osJ \ll Ine c,l'\,phnc' io't u' Jer one mol

ra evoG List\rieLdins,

qrLy BaNring, ins!-lesl 3nd disw esislst add qd$ 6rtoa

vdelies. The hsLilule also pmvides co on produclion technolosv lo the grow6 foiobur,.p l''sh.' \,eld:. Iach q.r.n ol $e lr'ri'ur '' tad€d bv /'n''I''l scEnilic

omF6'"\;de;ell qu"hficd, nJ.. lun" erPericn.e a.'\ed rb'rrn"dl r'''nirr;> lo'd

md abrc.d. The Inslnnle possesses lrore lhan l0 tuseitrch scicnlkls tnd labomloiies de

TbeScie'rstsoltb'rsrn$nrteweFlerymmhintolvddinllieptonrrionddnroiivdionofcofton crcp ih Brlochhbn Tbe insritft n slill Provid s tcchdical seFices lo the

Aericultu; depehrenl of Balochisl.d Covernnenl Tbus out staif h verv nuch fasilir to

otton gNwing m$ of Batochislatl

Sone olhet organiations lik€. Nuclcat Inslirure ot Asdculnrc dd Povincial Aericultue

Resarch Instiiulet e also wortins on coiton and olher crops B{ none of lhcse

ontu'arion( re d. rullnedEe a. Coron Pc J'.1 lr'rirLre s''Jn qirh all'olareJ

ree-cn,i r'pt-+ Howe\er' coord'L"on r' L J oe de\clon'o qrh ol er orcd'/drln ror

Ae exchdse of id€s sd dncussion

cotlon R$earch St tion, TandoJam

Co1lotr Rese&ch Slalion, fddoiam hd enliE infr.slru.rure incLudins hanpoeer to conduct

nrultifaet€d rcsearcn on cotlon poduclion undor vdious Ceosraphical condilions in Sndb lt

bs sufiicient laboralory spde, expe.inenral fms, warer and poqer supPlies ld carvoul lhe

pioposed rcse&h

')rogime There ffc l3 scienLisrs wo ins on difeftnt aspecls ot cotlon

Agriculturs Ext€n6ion DeParlrnant Ou€fl .' Bal06hislan

The Drcie.l is inteoded to b. lannched in cotlon srowing zdnss oiDalocbisttu' wirh nucleus

office ai,q.gricullUrc Dcpannent Ins ule Klmpur, Usla Muhanxnad. tsalochnlBn Th€

poi{t sbali be idplen;l<t in the followi.g agro_ecoloeical zones wh€r€ colron crop is

Nsimbad & Kachni Distrcls(nuzdd, Baebbd4 Nall \vadh and Ktrklr

De.a bugli dd Sibi

Kndan, Turb.t dd olher areas ofMekan



aal

arirc|'d Are, t000 hecr.@)

725211\

AGRICTJLTURE EXTENSION DEPARTMENT, NWFP

E*lesion $.il !p b $€ Ubion Coucil n nailalle to inPlen'ent the Pbjcct in the Dl. I<nm

dd Dau are.s. 'Ihe exisiing adni.isrratire aoansemenrs halc thc capa6ililid 6 i$plem.n(

It. DESCRIPTION OF TIIE PROJECT:

a) Giv.bri€lLilrory, propoled trciliiies rrd jutiffcrtion orproject: ildicrie tleb$i! for ielating (n. .rd of r*.rch and j$ti$ thc pnority lhst should be

siven lo Oe rr.r. Indic,te the b€hclitr ol r€selr.h lo tl'c sector add the

orierEi3ron.!d J iiticition

cDtbn lerl cud rtus (CLCV) vas oev& a p.oblen i. Ptlisran befoe 1988.89. Itws nsl notic€d in 1 968 tud €mdrged as a pobLB (989-90, its i .st:lion s?reld

a.rcss co on bell oldE Punjab and duaged cmp durine 1992-91. Txe counlry blssuflsrd a lossoialoutRs,70billio. due to lirus irlcslalion.

PRODUCTON IOSS DUE fO COTION L€AF CURL VIRUS IN IHE PUNJAB

{1963-2004)



aC4unrry b€cas nct ihronci insteld oi ner e&one!. lhd c?bnrophic effects of.l ( v looh.d o\er (dron podu.ron f'r ls) a1 oqw,r"

corroN PRoouctlol'l rN PAKISTAN

The spEad ol CLCV was chected vnh $e relase ol resisrrnl varieli* ie,clM,l )00. c1M.448. L-lM-446, ClM.44l, CIM-482, CIM'47I.FH 900, FII-901. FH-

614. M-53 ed DE'l18.rlowew., t€ resisFn.e decnarisn of lhcse cuhivds hdhren tNk€n dovn du{ 10 r1nat€d liottpc oi CLC\, connonl} called as Brewala

s|{sis of s(d \irs (ESCV). lhe resugchcc ol CLCV in lhe slapr ofnore v'rulenl

0rr( cdls for sgesl o(endon 1o fight bdd Dn {!. footi4 basis Moreover, inlensile

Esearch is rceddi to usd.hl$d riNs tamDitsioD lrcm cDiton Pldl to ratiou bosl

Dldri snd vie vds, otouilorins oi ins.cli.idc relnbr.e 10 tbneflI (tcz,ri,iaradt) ed {ftely popuialion d}nsics. lhe jesuigeNt ol CLCY mat .ot be

dudied in lanial bur in rotalityThftefore, resedch is needed to detedine ,srononitdhural practices. whiteflv

ndaseoent, insbricidt Esisla.ce to rileny, ud nutr'tioml Rqunene dlong

with deleloplEor.l BSCV res$&1)aticlns snploynrg conventioml and hodehtehrchgies lo be adalub{e i! ldious aBro clinalic coDditions ol ire pmunces

DESCRIPTION OF IHE PROJECT

t he ptuject wi[ n]cN id gtMd .n the follo{ine issu6:

L Stdt ot epid<Diolog oI lhe diseasc

L Sc@inB ol gmplbiL Slsct ed nonilorine of lhe diiease

4. td€nrifielih of go$ sd Elca$e of vari.tics rcsirlant td BSCV

5. Cstron af vdiabiBy throush redieliotmnuiidtr6. Use cf sole{l$ cbtcclerialion ol }nus7. IingErllinriq ol dton varie{ies

8. Managehedt ofv.cbt (whiteflyi

9. Ddvelopne ornars.nont (alegy ofCLCv diaajrI0. DirseDidalion oftechnologix to the fsms6



PLAN OF WORK

'In€ Injlnudon-wise derailcd phn ofwork is given as unller

.I CENTRALCOTTON RESEARCH

INSTITUIE, MULTAN

Colton failure or poor yields trc oiien oltcone of biotic and abiolic sriesses experierced bypldN duing lheir life spe. Amone de biolic facto6, cotor pla.r has been uder diEctthreal of Cotto. Leaf Cul vi@ (CLCuV) ard vhilefly, rhich is a vector cfCLCuV.

CLCIV w6 firsr noled i. 1963 dd eDcieed as a prcblen dudns 1989-90 Ils infeslalion

sprad acrcss otton belt ol lbe Punjab and ddased crop duillc 1992-93 ed in lhesubsequent yds. In 1992-91. about 0.89 miLlion hectecs were bsdLy dcnRged aldproduction dopped 1o only 6.5 aillion bales in lhc Punjab. Ccntal Cotlo. Researcb l.stnuto.Mulran developed six CLCV rcsistant varjetiesi CIM 1100. clM'448, cIM443, CIM 446,

CIM-482 md CIM-473. Develop.cnr of CLcv ftstuanl ldieties resulted & aleraseincredc in rhe produclionofl.2 nitlion bales woft Rs l2 rrillion per sn$

Keeping in view, lh€ sevdilr of lbc problem, a.d to susta rhe econody of dre courl.y,Oovcmnent of PaLslm sdctioned Droiecls {orth of Rs. 2:19284 nillion Io @nduct

resdch or variou asp€cts of CLCV d6sase.

Tbe utiring Esearch eiforrs of lhe CCRI, Multal scienrisrs resulled in tnc clolution ofCLCuV resisrst varieties The Lstillre is the pionett instilulion lo evolvccvd firstClcuvqistst !&i€1y CtN{-1100 rnat DBled sry for $e dcvdolaBt oi s@c€ssivr CLuV

Fsislmt vri€des intne

courY,Reehny, colron leaf curl lirus bas.gan eme4cd a, a key dise4e in lhe ptovin.e oflunjabin g.l$t sd Bu€wala .g nr 9s(alqr. T\e te€sere€ncc o{ lirus \as dbgetols trsiordd colld develop inlo a serious o$brcak. Il is sspected that previous vitus st6in hamuhled. MoEover lhe prBenr resistenl vadedes and semplasm have sbown su$cptibility lo

this n€q st6in of CLCuV disese. lheefore, sdiols efons ee needed that CLCUV

Esisl3n e f6n Colsrtiso rrtorih 10 Go$)pih ttr"l,Dr be tdsferRd b nare isnusii)lsaimtthisvirus. If researchqorkisnotuhdedaken,lnecounrryqillbearslake.

Theicfoe, lb@ is ar n€e need tlal inlegraled and inledisciplindr rcearch be mderla&eo

lor develoDwsl ol esisl6t !ai.!ies to \his ne! siFin of CLCIV dissi The rsdth becmied our on wd fooring before lhe problem sea &ngerous dnnensions fld hunirs the

narionolec.nony, rhich the.ountry exptrienced catll€tduiDs l99l 94

A( {\e pJ€senr no er{, CCRI, Mllran l\$ m€aAs raouces ro combat lh€ dEBing pioblmof Buewrla CLCuv disese. we hav€ ihitiied rcscaich wo in this dnection:

i) Scr€ening of gemplsn ro searh oul rsista.ce asanN iew lirus for irlhctddelopftnr of €sisssl lar€ly



ii) keping,atch aod wad oails funher severiq lltrough nonnori.g 11'e collon bell arca

padiculdly aourd DuEwala krilory.iii) (.leslisation the cause of ils csugerce in Burewala aEa.

iv) Stdyins the cappine pa(m 6 w€lL as t.h.vi.u .f presd esisle( \$ieiies in

Bwwals rea sd developrent of slrat€Bics for it manasenEnt.

How€ler, rmdidg is requn€d b conducr rcsarch lo nelt this nenacc. The &se{cbpmeillme 1o be cdried out $ s mdei:

131Year lln Dec.mbsr 2002 to 306 Jun., 2Oo3)

Cempldd lill be screened aeiinsl lhe new stuh of cotlon leaf curl vtus Tbe

gedpldm found resislant wiU bc used for crossins purtioses in lhe glashouse to lmnsfer

vids Esista.t eenes in adaprable local variclies of upland conoi (G hnlutun L.).'lhecrossed seed will bt lldted in lhe Fl ecneration id tbe fi eld to raise F2 seed.

lde iicarior of Fv desira6le ispeciali) CLCuV €sisldce) sen€s fq rtusfet $cultilated coltoN. in wild ed cultilared species of Cossypiun and in relat€d ecnenEvedmenb on pleting ne6od and nMgcm.nl or itrisalion and lenilizei

Cropping paltern studies.

Popularion dynmics ol, t/raci on altemate nosl plants taoh colton srowita &cas ot

Multan. Bur€mla sd vehdla) ldenrify the supecled host pla sofCLCuv.b) Population dy@ic ofA rarz.i oo alletuale host plants.

Screening of sftaintvarieties for blerance/susccptible to a rord.t and CLCUV

MornornB of inseclicide esisldce,

Srudies on nubieht indexins of conon i.fcclcd wilh clcdv lo dtt€m E lbe.alue dd

severily ofnutriliod dnorders in difcienl agro_clinaric zonesField elaluaioo ot @nnercial and advmccd $tains fot lheii response to clcv dhede

uder D.l. (lrm envnoment.

Vite up oflnitial ReFrl.

. cmplsm will be screoed asrinsl lbe new slrain of cotlon leaf curl viNs Thc

qenDlasn fonhd .esisrant wiU be lsed for crossnB pugoses in the slassnousc to lrmsler

iitus r€sisrlnt sen6 in adaptable local larielies of upled cotrar @ hnsttun L) hecmesed *ed will be pldt€d in the F I geneBlior i. lhe ficld lo nke F2 seed.

. C drdu,, (A-eedone sp6iet. EsisLnl m$s nmuic to collon leaf curl viru, Niube

crc$ed with GdrDer,, e.hartnetsii, G tldritlenii, <i raino"dii et (D'g.none

spdies), s Garb,re!, does nol co$s dnectly wilb O r,tr,/,4.. Ths crosscd seds ofi.l.rsp*ilic hybids Nill bd eroM iI pots ed their chrodo$me

number will be doubled (2n=521.

r Ide ifiqtion of .ew destable (speciauy C!CuV rcsGtdre) genes for tmnslar to

culrivaled @nons, in wild md cuiivakd species ol Cosslpium md in rclated 8e.era

. Field experiEents on agrcnomic requiEmc.l of Pesent ad new CLCUV eshla



. Expeimenls on pldling nelhod end Da.agenent dlnrigation and feniliTf,r

. weed nmeemenl studics.

. Crcppina paltem studies.

. ExperinEnt at fames fields in lhe conon beh

. Populalion dynmi.s of,4 /aba.i on allemate host plants fron corlon gro*ing ar.rs ofMullan, tsuF*ala dd vehdic) Idenrifythe suspecledhosrplantsof CLCUV

d) Populalion dynoic ofa rdba.,oh alremak host pla.r.. Survcy of crops, ornmcnlal. rruit and foE$ plants aDd r€eds fot the susiral of,

/dra.i\ mlural enemFs liob the cotlon Brovnr8 deas of Punjab. Sodt then biolosyaod ecolqy in lhe given enviomenl

. Tc inlesligale hosts which odd serve as resenon for rne hultiplication olpromhinspaasnes and their shining lo @tlon.

. Sc@. idg o f slrainvvdieties for lolcrmce/suscepr ible lo , rabar; and CLCuV

. Mon iloinc of i nsecticidc rcs islance

t Snney vill be co.dlcled in couon Crowing areN to rccord incidence & i ensity oa

corton leafcul lirus on suceptiblc as well as eshlanr varieties oaco[on.. ThesyDptonsofleofcutl virus will be obseryed o, ollEr pl,.ls. Sludi€s on.urrieht ihdexing ofcotlon

'nfccrdwnh CLCuV ro d€tcrmine rhe mlure ed

*verity ofnuhitional disrde$ indiferc.r ogm-clitraic zoNs. Filal exrE.inents lo study l[e inleraction of nilLoaen a.d pota$iun nudion in

ft levdce to incidence of CLCUV disetue and couon poduction.. To detemine the.elatio.ship b€tween nurient indexnrg md clcuv disease.

. Field €valualion of conn4ial dd advarccd nrains for rheirrcsponsero Cl,Cv disea*nnder D.l, Khan envnonnrenl.

r Wrile up ofAnnMl ProCress Reporl

. flre F2 seed qill b€ pldted in lhe ficld in F2 geneFlion for sclection of desiBbleeereSates. Tbe seleclion for virus resistahr plrnrs will be made rnder natural infestationsnditions $d dtiiciBly ct€alisg the tlNtalion of inlificarion lbneflies (Denisia

tabaci) to lrosmn de virus.

' Difercnt cultivm of OhB,/,D, now susceptiblc ro CLCuV will be oossed withinleBpecinc hybrids to bting al lelFploid lev€l (2n=s2)

. The oo$ed reds (Fl) will be so*r id glass house for *r€cning of CLCuV resisr&t

hntanrc and back cpssed with 6 hittttn (RC 1).. Developncnr of Bt ed other traNseic corton r.sisranl to insecr pcsrs and diseases witb

h igh yield and desi 6bb nbE clDracleristic s.

. Field exp€linents on agronomic requiremeDl of prcscnt Md new CLCUV esislmt

. Experinenis on pl&rng nelhod aM Danagenentofirrigltion and feililizerr Wed nanagenenr studies.

. Croppins paltern dudies.



Experim.nt at fameu lields in lhe coltod belr

Popnlation dyhdics of , ,abaci on alteDale

BanawalPur.

Tq inresliSate hosls Nhich could sene s res€reotr

pdasils ed 1h.n shinihg to conon.ScFening of straindvdieli€s lorbl.Ence/susccptible

Ell€cr oi insdt eostl tellatos (lGR) ed diilercnr

l$sr lL/nts fro'n KhaoewsL tuad

for lhe Dulliplication of pronining

groups ol insccricides on , taldct

Modbrnrs of iNe€ticide resist nce.

Swcy vill be conducted i. cotlon sDwins &eas acd i.cidence & intensnv orcoton

l€af curl virus on suseplible as sell $ resisldt vdieli€s ol cotton. Abo n.d the

pFlaleoce and spesd of clcv lo other geoctaphlcal ae6The syqrlons of leal clrl vitus {iU be obsened on othcr Flmts

Studies o! nutriert indenng ofcoton infected {ith CLCuV lo delennine lhe natG and

severny ofnudional disode$in dinetntagm climatic zoncs

Field expeimenls b evaluale the conpdative elicacy of folie leedi4 ofNPK, B edZn vesus soil appliqtion in relarion lo CLCuV disase

Mulliplicalion of CLCV Esista.t vdi€ties undo D l KIan envnonmenr

Wrne-up ofAflual toer€s Rcpon

. Selecled sesresales will be planled il fr gcn.rati.n rhe lm€s selected in [] seneralio.

vill be pla.ted in 14 seneration it rep licaled p rogenr @* t'aLs

. Bc,l nuterialwill be toM.

. sc@ringaeaist

Clcnvmd back_crossine $ilh G,r/rlrd (Bc_2)

. Oplini;ion of pDloel for rmsfer of these senes to lhc cultivaled coto's bv using

biolahnolosy. D;v€bpnell ofleclDiques foi rceeneralion ofcotto. llhlJ llon coker-

ll2.l15 md loal genolypes

. Fi€ld exgeriMrs on nsoqonic €qun*e ol D€sr 3nd ncw CLCtv €sisrat

. Expenmenls on pldting 4ethod ed mdlgeneni of iBisation ard fenilizer'

r Weed Dgnacenent slud'es.

. Crossi.g palt€n sludiss

. Expe h€nl in lame^ lield

. Screeni ng of sl.ai n$dieties for 10 lerance/susceflr bl e lo /t rz'a'r:

. EfIecl of irFcl sroMh Eculalor (lCR) and diIIeE.t srcups of nsecticides on , rdDltt

. Moniloitc ot iBediode r6isloce

. Dsd on Esults delis. teasenent st6resy.

!) R€duclion in CI-CV inoculun belbF and during collon season

b) R€duclion in pesicde use

. I*hnnalbrcchue on Meagenentolcorlon leaicurl viu reetor Benisia labacit'



. Suvey willbe conducEd in cotlon srcw c dcas rccord incidence & inte.sily olcollon{€f cld viN s su$eprible as well as resislanl ldi.ris or cotlh. Al9 nnd ficpralence md spEad of CLCV to olber eeognphical aeas

. The synDloms of leaf clrl lirus will be ob*ryed on otlEr planls.

. Field exDeriments to elalutethe conparative eficacy of foliar lecding or NPK. B

odzli v6us soil application in relation to CI-CuV diseas.. Incorporaiion of Fatt Ydd Mmure alons silh elemenlol sulpbu ro inv€sligale $e

incid€ne of CLCuV dise& under 6eld conditions.

t cr€d hous. srudi.s lo est.blish nuirienl deliciencies in coLron on lbe b6is of field

. Multipliqtion of CLCV Esislmt vdieti6 for provision of qualily seed in D.l. KnM ea.

Ilii4iiq

b) I€ining of f€ld stafi ol pesticide @mpei6and pest cide dealec

c) Trainlng proq€mmes in colaboration wilhOepanm€nt olAg cu tur€ Punjab

83gDg!I{--Erss!a449!

'i)iDProductlon of Resou..€ tl.t€.lal

a) Cotlon Guids Book lorthe Extens on workecbl Cotton Pamphlets, Booklets, elcc) Guidan€ thDugh the NeGpapored) Popularadicles in fie NewPaPeFNallonal and lntornalionEl S.minac

2500

30

150

3000

150

3500

No. of Proqr.mm.! p.r Ye.r

2A

5

1102010

25

20

50

1

102A

10

I

205

1

t02A

10

1

wrire olFrnal Te.hn,ral ReFn

Oulpul ofthe Proi€.t

. The gemplsn idendfied as CLCuV rcsislel will bc ulilized in cmssing poeranhe lor

the delelopnenl ofhi8l' yieldinC CLCuv esislmt comncrcial .otton ladelies.. Resntanl cempldd sill be utilidd fo! the derelopdent oi CLCUV reshlant comnercial

. Studies on rhe nrechrism ofinheritance ofCI-CuV vill lead lo develop more ErstaItllines vilb other desnable chdacters.

. Resistanc€ of the wild C,rs/p,!u species asainst CLCuV Fjll be incoQorated in tne

. Cylo iruleial delelop.d thtrouen inG6pecinc bybriJizarion wnl be screened,nd lcsted

agains clcuv sd oiher desnaue yield and tbie chersderistics.



. Conon culti!&s obhioed rbrough bioleclmotoel qill be cxpccred io be rcsisldt to collonleaf curl vins sd bollwoms. In Ihis way billions of foreign erchanse *ill be saved bylhe increase ofcolton production md nidiRizins ltE cosl of todurion.

. DxistinC cshldt to CLCUV veieties will be restcd i. urious cortoh gowing ares ro

dekmine rheir F.ponr roCI ru\ d.Js field londi o'.. R€sistaht vdielies of cl-cnv qill be rcommended wirh coDplete packase of ptodncrionlechnology to be filt€d in collon croppinA sysien.

. Delemination olsuitable line olso ns for laions cl-clv vaieties vill help nr lbenmaa€ment of clcuv problem.

. Raonnc.dations nrade fiom varer.nd linilizer srudics will help inlhe mmagement ofwhit€fly, whicn is lne leclor ofCLCuv

. Re@nnedations madc tsh ueed mmoecncnl sudies will bc adopled by tn€ fdre6for the control ofweeds in oder to clEck md sprcad of CLCUV

. Coltoh coppinc stldies will facililate lo check md spread oi CLCuv.

. Studies on lhe populalio. dynmicsofwhiiely

andmonilori.g ol

resistancewill l€ad torhe be(e! \dqsiandiog .f (he CLCIV 3ft1 lesr lonrrol neasses These srndis Dill aho

facilirare in rhe mMgenent of whnefly (vector of CLCUV) for the dcvelopment ofcontrol shategies o f CLC uV.

. Nuririo.al studies will h€lp ih undeBtandina clcuv disease auack dd ik severity.

. Studies on tbe nulrieol indexins will help lo hhase CLCuV dis6e.

. Experinent on chediMl basis of rcsistancc will belp in urdersunding resistarrDaheisn ofcollon leaf curl lirus.

. Prelalence ed sprerd of virulent srlnr {iU bc dekfrnred for funher CLCuVmoagement srategi6.

. ldenlificationof .ouo. leaf cudvnushosrpldruill facilirate innmasencnrof cl,Cuv.

. Rcsdch sodies ofvdieties in dif€€m corton areas with respecl !o Cl-cuv will help inlh€ rccommendations of valielies for diff€.eni areas.

. va.ieties showins tol€rance 1o CLCV qill b. idcnrified lor the parricultr deasr li yil help 10 ]edDe the yield losses due b Ct-Cv nrieclio. in $e couon srovine &eas

r Resqrch bsed infodalion developed thbueh CLCV pFject on lbe manasenEnt ofotlon leafcurlvirus in|he@untrywill be disseninated to vdious laryer sroups rhtuugb

hJl'imedia inlesEEdspprodch .le(xonic and pnn' m, J',

NUCLEAR INSTITUTE FOR AGRICULTURE AND BIOLOGY (NIAB),

13rYoar{l'' O6c.mb€r 2OO2 to 3Oh June,2O()3)r Induced mulalios.. Hybridiation dd other teclDiques

. MaintenaM dd mdtiplicario .l rc$ stljl of conon lcai cud vnus under controlle!



a

. Ml sedwiUbe plmled es M2 populatior udcr high seleclion ptcssue Seleclion inM2

will b€ cdied ;ut on the basis oa dheasc resishncc vith desinble norpholosical

chadclerhtics. lri sh yi€ld potenial and goon nbre qullitv

. Malue !'./M. dd.esulrins lron inle6pe.it' crdsscs {ill be utiliz'd b iais Fl/Ml

. il Ml epnoope. $ill @ evdlured lor $eir morphJlosi.'i marrabie pldr ramp

rotrace aens;ri, boll erention.l {de rdr'ber' numbe' ol Jtls blen lor boll

r"miLloo naruarion, e.ponsro niln r.mpe'alur.ddCl CV di*d'. Reshlmtlole€nl natetial found dudns fi$i vcar ttuough sraftine lill be llmted at

Buewala ro see iB espome 10 conon leaacurl lirus under naluial condilions Graiiins ol

oew mllets / stnins will also be P€lfomed NIAB lo find oihd lrighlv ressldl

malerial. Subj.cli.g lhe plmt population to diffe€.t deske of sel{tioo pressue {illmske rhe sel;do;of d;na6le resista.t Farerial The pq'posc w'll be Io sc@d fdseneral aod specific tol€r&c

S'nsle planr progen) tusi or rh. clre mLrdslri.rn' sirl r errlted srth BuFwdla

qninor.olbnledlc l\iiuq b li.d re'isLanr/rol('a'l .uuRe

. DesiEble egresdls elecr€d in M2 gere6,iD, rtill be used ,o tti* Ml senetalion (rcs

. One loculc bulk frch promhing fUMl genoilpes wrll be uilized b advmce the

sesEsali.s seneratioc (t2lM2). vl*d eJul,De rron airecr imdistion of seed will be urilized 1o raise M I seneiatiotr'

NATIOIIAL INSIITUE FOR BIOTECHNOLOGY AND GENETIC ENGINEERING'

FAIShLABAO

1'r Year (ld Decomb€r 2002 to 30s Juns,2003). Coll€ciion of gemplsn of diflerenl cotton species from varios souces

. S.Ren'ne ofdaEoe lor drre,enl (now ri tuigene

. cloninc of vial conponehr i.cluding DNA A, DNA bela

. Availdbility ofeCuence infom,,id sbou! ecnes cotfding tesislerce ngainst difere't

diseses in cop ptdts.

. Cloncs ol all conPone.ts ol Bur€wala cotlon leaf curl lirus

I Sudey of cofion sosi.s rteas and sadplc colleclion

r ScreniDg of difierent collon species .3ainst Burewah lnus disease to hale ctosses



a. Dgsictrinsofp ne$ for RNAj .ohrlrucrs. CloninC ofPCR mplifiedproducrs in RNAi veuror

. Tmslomadon of conslrucls in,4s.,rz.r?rt,- md lraDsienl assay.

. D€velopment of infecliou clones.

' Thnsfomaton ofgene conslru€ls in cop plets

. Dvalulion of tFnscenic p)uls ior €sistece againll disease associaled witb wbilelly_

tdsmitled geDiniviues.. Synihesh of DNA prcbevpriners.

. PCR mplincadon of genonric DNA in collo. {iIl) $c spccinc prineis

. Clonins oI lhe mplified DNA poducl inlo a !.clor for DNA sequenci.g

. Scming ol DNA of tbe selecled species with RAPD, and SSFS lo nnd DNA

. Dsrlblisbmeot oflbe nostrobusl DNA llnBcQtihting rcchnology-- AFI-I

. DalaEedditc lhe dheas€ r€sponse ofdiferent couon sFcies will be available

. Synderic allolclraploids vill be d€wloped

. Complete mde6t ndins ortbe causarive asent at nrolecular level wlicb will hclplo

develo! lesl lrd plolidi eeoes hr 6is!!B.. Delelopme.t of specific diagnotlic took for thc bEcdcs

'.dcl..r Btrrew,la vnts In

.ol|on, aliernale host dd shi&nies.

. Availabihy ofdala on spE.d of Burcsala virus i. olnd coito. eowing aEa of Paldsh

. Availabihy ofcene construch for blocknrs virus rcplicalioD of geninirirus or lh€

. DNA squ€nce infomalion ofde cloned frasmeDh of colto. will be available

. Polynoqhi. DN,^ mtrke(si donC the selected colto. species will be .vailable vhichcould be used 10 difieEntiate tho specFs

. Conrinled tmfomalioh ol ge.e consrucrs in fop Plmts

. Eval@rioh ol tmsgenic plmrs lor r€sislance again$ dheases associared wnh whireily

lncnitled Seniniviruscs.. D€velopment ofF population.

. screehingoflh€ sesrcsalins poPulalions aaainst the BureNalavnus di*ase

. Confimation oalinkageofthe DNA polynorphkmt with lhc disease Esisl&ce

. Codrnction oflinkage maps with lhe lpplicalion otbioinfornatics technoloev

. ScEening of lh€ F, poPulation lith DNA probr:s a.d pflmcs

. conpsrison of th. pubrile r€sishnt genes {ith othei crop pldrs.

. Tmsie say will ssess usfuln€ss of gene colstrucls lo block virus rcplicalioh or

. Tmsformalion of co.structs in cotlon siu prolide $urce of genelicaU/ e.sineered virus



a. Cenone napping of tsist nl gencG) ag0n$ lhe Buieqala virus discasc

. Diferc.ces of the putaive Estuant gene *ith senes conLdrs resisra.ce againsrdifcrc.l di*ses in ditlr€hl cnp phnts

r sc@ningofC.netiallyensineeredrirusLeshtanlcoltonplantsunderfieldcondiriotrs.r Populalionadvanccment

. ApplicationollhelinkedDNAnarkerinDarleFa$istcdc.tlonbreedingp.ogFnN

. Ayailabilitt ofvnus rcsisldtplanls lo breede6.

. AEilabilily of rcouees and mlpower ro uhdcdalc dy projecr Elaied to inlrcducine

senelically en8ineeEd chdaclen i'r colto... Availability of DNA narles lo diileEnt Esearch ot8aniation.

COTTON RESEARCH INSTITI',TE, FAISALABAD

1'rYear(l't December2002 to 3o'i June,2oo3). Sufley colleclion md identificaliobolparasires and predarorsofvhit.llyandothr

. Scening ofgemplsm in cEenlouse nr Vclari.r Closiry for sene pyrmidina in s@dl$use at Faisrlabld, Mullfl, Bahowalpu 6d

. Fs|er advdcemenl of lilliel generadon nrsreenhouse

!t Fahalabad, Multd, tsa|a$alpur

. ScEenincof lieldresislantsenolypes rhrcugbwhirellyarveltari.

. lnlrsp{ific cosss b€tw€en C. nns um and Cossypiun arboreum

r Record disease nrcidence fld severity

. ScE.ins ofl2l0 Colton accessions a8anN CICV al Colton Resedch SIation. Vehri.

. Sludy of 1242 eempldto accessions d research slarions located at Fakal.bad. Sabiwal,

Mult n, Vebari, Batawalpw fld R Y.tudr fo! CLCV resislance md olher lnits.! SrrJy o' Io1 Fl !ms*. lor lombin'ng

"bilr)' J hrb"d \'ts u

i Sludy of 165 F2 cross€s for selection oi plec resisrant b CI-CV and posFssing good

. Study of 1928 F3. 727 F4, 388 F5 fld 75 !6 pogenies for CLCV resnldrce ed otbcr

destable ago.omic trsils.. Study of I I PYT &d 44 AVT lmils tbi CI-CV resisrece and other agrcnomic lnits.. Study of l0 DGA lrails for CLCV Fsist$ce and a8rononnc lrarls.

. Studyof6NCv traih lorCLCV resistdce and other agronomic rBib.

. FibEqulity evalualion of 1000 enties.



a. Developheol o1800 l@sh crosses for dE developnent for pymnridnrs Eshlar senes to

cl-cv.

' Plmtins of 1000 filial seneFlion entries Esnla lo CLCV for adrancine Cenemtion ih

. Developmenl of 50 crosss bet{een Desi couoD (C d/r,/.,,, dd uptand Cotlon (C.

. Sw€y of CLCV incidence.t th€ ftrmels ficld.

r Dev€lopme.r of 1000 t*h crosscs for introducing cr,Cv Fsisrdrce in hish yi€ldinggood 6brc qualily colton v&ieti.s.

. Study o! 100 F2 cosses. 1500 F3 progeies snd 200[6 pmscdes aor rhe devetopheol of( LaV resNhr. hrgh )'.ldrn8dd bere qrd.rr f-'rur,'n-

. siudy of35 PYT dd 45 AYT expdimenrs ror CLCV resklance, yield and nbr quliry

. Study of DGA lrials md NCVT for CLCV resktance, yield and asrononic lsns.

. Advecins ofelite fi |o Fi Ccdcmlion clcv .dis&nr n,arerial in s€en taur.

. Screening of alailable gempldm of colron asainst CLCV ar Coroh Reedch Shrion,

. FibE qulily evalualion of4000 slpl€s.

. SurveyofCLCV incidence at$e lamert neld.

. 20 out-reach vdiet l raih for noniloring CLCV incidence.t Development of60 crcsses bellen D€si co[on (czl6orc!,) ed upland Cotlon

'IL Sludyofsu(dsfL inter-speific hybids.

. DereJopDor oi 1200.rNes fo pyraDidir€ diffdsl .oebidlio, ofresirot goc.

. Study of400 r_2 cro$s, 1300 F:t progenies,900 F4 pLoaenies. 500 F5 pogenies od 300F6 Fosenies for lhe developmert ofcl-cv esisl&t tines travillg hish yield ahd be(er

. Sludyol4200linl sahples for fibrc qualiq tnits cv.luatron

. Study ofd6i couon (c z/bdrd,,,) x upl,nd cotton (G t'6!ru)

cros$bred naeiiat.. Sodyoainte.specincsuccessful crosFbrednateriol. Study ofNCVT and DCA rials for cLCv esnhnce, yietd $d quahy atftibutes,

. Studt of,15 PYT and 50 AYT for CLCV incide.cc. licld and tibE quahy ftns

. sutveyofCLCv incidence allhe litmersfieldt Muldpliatioo ol CLCV resisrmt mal€lial.

' Screeninc ofall @uo. nalerial aeai.st cl-cv ar coLlon Resedch Sration, V€bad.r 20 oul reach v{ielal kails for nonilorihg CLCV nrcideDce.r Rapid multipliqtion ol CLCV iesisbm h,reriat i.e. l'3 to 16 gcncrarion dd advaned



aDEPASTIIIENT OF AGRICULTURE (EXTENSION & ADAP1IVE RESEARCH),PUNJAB,LAHORE

1'tYear(l't Decembsr 2oo2 to 30m Jon6,2003)

. PuFhae or vehicles tbr DOAS dd DDO S (Exl) for cxrensior actiln€s.

. Proririun ofVororJ.les ro ao. on h'r pu(hs.e bdn

. Prihiing of lilcmtu.e ad pubUcity

. Piepaation oI Fli? Charts

. wntins ofAnnual Pogre$ Repon

. Printing of liled@ and puhlicity

. Prepmlion oa Flip Chtrls

. Wrnihg ofAmual Pocres Repon

. Prihline oflit€ratu.€ fld publicity

. PrepsationolllipCbans

. Writinc ofAmualP.osless Report

. Prinlincof lireralueandpublic y

. Pcparalion of Flip Cians

. Wriline of 4.6{.1 PrcC€ss ReDo(

CENTRAL COTTON RESEARCH INSTITUTE, SAKRAND

13r Year (1'' Oecemb€r 2002 to 3Ot Juno, 2ooi)

. Exiensive sw€, ol€nlie couon beltofboth rieprcvinces wiu be ceried-out for CLCVmd ns v€cloB in cotlon dd ahernale hosts planb

. ScEenihgofcouon Semplasm iahol spols ofCLcv wiU b€ canied ourr ldentincatun of vecto6. rlFir Mtural enenies. relalionsbia between lbe vslor and rhe

' Extensiv€ swey of ende cotbn belt of both the Flvinces will be cdried our foi CLCVdd ilJ lecton in cotlon dd alre are hosrs pldrs.

r Vistu of CLCV bot spols i. lunjab wilb special Elerence ro BuEwdla virus to getfamiliar with lhe hod€ ol atlek. ddase synplonrs, crc of rhc virus.

. Screenihgofcollon Cemplasm in hol spots ofCLcv uillbe caoied our



a. Crossing of CLCV r€sist!tr1 lines wirh well adaptd and hieh yieldihg stnins deleloped

ar CCRI-Salmd will be aredpled.. Identification ol ledoB, their nalur.l enenics, Elalionship bel*een lhe vstor ad 1[€

. tfrecliveness of bio-contbl ag€nr

edinsccricidcs aeainsr rhc vccror

G) willbe

. The virus-afected plels qill be screned $o!sh ELISA ten for lhe ielation liius

. To screeh lhc bre€dine maleiial by enftinc or n€ck buddins.

. To obseFe the atlack ofCLCv in relaion lo nuririonand irigarion doses

! Thc rechnical slafwill be sent for hlnds on rrainnrs in localorsaDiations

. Dxtensiv€survey olCLCvwill becontinuedlor$e secondtetr.! visns ofCLCV lo( spots will slro be co nrred

r Sceninc ol coton gemplm ioi CLCV resistance Nill conhue. CrossioC proeram€ wiU also onlinue.. Fl nateial developed in 6e ntst yer will be scr€ened in th€ clcv bor sporrr Other studies cadied-our inlne Insr yed willbe rcpcated.

. Pesl md prcy relaliomhip will aho be studied.

r The t€ch.ical salfwill be sehr for haideon troini.g in local organizrions. Shorllem Fconoedalions oo lbeb6is ofstudies cried-out so far, wiu be

dissenimted 1o tlt $eaohe*. extension {oikers Md the giowe4.

. Exrensiv€ suryey will also be coodnued for rhe $nd year.

r Vhits of CLCV hot spols vill aho bc conlihucd. CiosihC prqram€ wiu @dnrue.r FImate dldeveloped in 2rd yetuwill bc sciccncd for CLCV r€sisrd*.. P2 natdid nevelopeA durine 2ad te!. vjil tunhe' b€ s.reoed b'.esisrin.e in CLCV

r Fi.al report writing, @onnendations aM fulure plannins ro manage dre problen will becomlnicated 10 the quarlen concenred

. CLCUV Esisrant ldieties sill be developed.

' Identili@tioh of vedoB, rheir mtual en€nies, ElatioDship b€tseen veclor ed virus will

. Efstiveness of bio-conlrol agents ed insecticidcs against the ledor G) vill be

! Alract of CLCUV in Flarion to nubition and idgation {ill ielp in nininizing lo$es due



COTTON RESEARCH STATION, TANDOJAM, SINDH

1't Year ('ld D€c.lnb€r 2002 io 30bJune,2003)

ISuney of tne C|CV ee. io Sldn lnd runjab

. S.knirg of Cedpl@ al Kol Chulam Muhaddad (Mnpur (hat' s,$rg .I clcv adreced litres in differeni cortoo sioving dislricls ot Sindh

. Jnvesris ehost DldtofCLCV ih differcn1 loc,tioos ofs{rdh

. Invesligarediferntspeciesofwhielly incotlo.srovingditrictsof siddi'

2"d Y6ar. Coll€ction ofCLCV Esi3tdt geftplaD ltd ro bc !own-

. To utrlie gmpl6& ir b'b.idizl'd,

. Surcy of corlor srD'i,g re. of Sindh {Krnnpu dislticL in upper Siqdh. H)dsabsd

sosld, Mirlu*ns dhtiic$ and pan of Koti dea in lo*€r sindhG) lbr colladoh.

tBse(ario. dd id€ntificadob of dilfee.t species of*h'lelties. Taxononic s$dies o. qhileny spccics in coltor

r To r.cod narurul eDenies of whirenies {nLl inllet of ,uCDenlriortr.redcs of

nrcdarorvpshiloids nt co(od .op l:r confol ,f thil.,)]. Ide.lificaaoo oaaltcndtelbn desof $il.ties in Sn h

. R.d.d tte popol.rion dl&Di$ aDd disrribuliotr ol v\iLcily ii dilGrcnl crops

. lvdlu,lidoftkllerlr,clsfotthcconlrololwhitelly

' sw.y oI6ton goMns &ea of sindh Kuitpur district in uppo sindh, Hyderabad

shgbd, Mirpu*IN dislricts &d pan ol Koiri 4ca in {oser si.dh(cl for colledion,

peewatoned ide.liication ol id dne&s

. Colledion otspeciDer loi tunner study ol !'rur.

. Wriline ofAnnudl Progtess Reporr

. Tle !1, rill be so\n in the nonlh of Februaq !r KoL G[ulml Muhmrod/ Nabisd

.:nreFlwillbepiclcdinl@e&d[2villberaisd0tKoldijiirlstvekoftune,indlesme yq sd s well 6 CCRI. Muhm drd Cotbn Rese&cl Slatlotr S.niMvveldi.

. lt will facilirat€ to eraluate tlre filial genedlion in datc€l itucoru& i. lol spo! do oIPuojsb from lhe infi{ $ages of brce{iing dalerisl becrusti $eF is to ho1 stol tuea or

CLCV dises. in Sindn.

r llybridistioo tro|l to be.dlitu.d.. Su6!y ot .olloD at€es of Sindh Gnairpur disldcr in lpper Snth. Hi-dcrabod Sdgl8r.

Mjrlur\bs distncts rnd Dan ol Kotri e! ir lo\eL si'rdht) lor colleclion ard

ideddncado. of difieEnt sFcie! of whknies will bc cohtqued

. Blological contol of shneily lhroush bio'ageN nr Lab ad field oldnions

. Identification ol altrrale hosl planG or{ltnetics wil] & .onlinued.

r Study ol lhe topda{od dFdiif of while0} e cotlo it dilfetent cologial zones ofsindh rill b€ ePedrcd.

. V&ielal re$istaDc srudis slaiost whiieflics nr cofon vill be @r(ih!ed.



Re latiorohi p of veclor vA dise$e i ncidehce.

Sudy of inpsl of mir crcpp:ns / t6p cops on *hite0y build-up in conon.

To l@rd latual eDeDies ol whnenies and angmc.ialion / relees ol prcdalors/paraitoids

in ionon cmp for control oawl efly.

I valudnon ofpld ourF ior rhe @nuor olqhicnj.Mirefly nmsene with difier€nt conlouihg techniques includng sacrificisl pldls.

SN€y sd @lleclion of sp6ino.Identili@tion of viru dis6e fron colleted sd.ples lhrough NARC

ScHing of gcnpl$m of @tlon against cotlon disses.

Ue ncation of lirus on oth€r Klt&ir crops.

Wtuing of^mual Progteds Repon

lnis F2 will be 6i3ed d 13 at l(ot Chdlm ,\lolmnad/l'J,b js i! te ,rorrh oJ February

F3 will be pick€d in ihe nonlh of June od raisd s F4 at Koldlji dd s well d CCRI,Multr, CRS, Sa,himl a.d NIBCE raisalabad for c*luating aeainst CLCV disede

Tne successtul material vill d$ bc t€sted tbouen gmning t€dniques 5t CCRI,

Mull,n dd DNA tes.t NIBGD, Faisalabad

Finally rhe r€shLn datedals will be lested in sleednrs Triah in dilerenl ecological bne

Adroced seneDtion of cistdl poee.ies vill bc bulked inro strains for nrclusion in

aesides, d€ present r€sklant mtedal oa clcv will aho be ptoDoted md will be tesled

wlilefly lif€ kble! stndisin lab @ndnions

Vdielal eistance studies will b€ condnued.

lmFcr olcullu,l pra.tic6 on Du.ld-tpol trturericc in oroPEpamrion ofshofilem edlongrem @ome.dalions for of vhneniesconlol.

Publi€don of one paper in vemacuh langlag€

Codpilaliotr of nnd 6ults of esedch work.

Reedch project will result in elolution md developnent ofcouon !&ielies Esisrd lo

Re€dcn on eoionological dd palhological sludies uill helP ih lhe develophe.t

stategy for mEnagemenl of CLCuV dise$e !o savc lhe counlly lrch loss in yield

Conon growen of $e sindh province will be lhe uhnnate beneficiaries i. tems

prevenlion dd onlrcl of clcuv disease.



AGRICULTURE EXTENSION OEPARTIl/IENT, QUETTA, BALOCHISTAN

Ech year @tton seed of CLCV tesisla.t vsieties vill b. supplied b lne growe^ ofBarochisld at 50 petenl subsidt in lne 6Ft year,

'10 percent subsidy ih second !€d, l0p€cnt subsidy in ihird y€ar dd 20 pereot su6sidy h founh yee Aeticultural machinery

will.lso be provided on lhe sade subsidy leleh as seeds. Baloclistan sov.rMent wiu sel up

a connitle uder Direclor c€neral Ag cuilunl Exlens'or to fde rules fldrcsulations/€rileta for eligibility to subsidized seedvmachincry.

fte dehh orAdoch$En Cotron pbglllme re.s iollJ\\

1'tYear {1'r D€cember 2oo2 to 30ih June,2003). Introduclionof cottonleaf curlrirusresnk rarictics

. DeoonstElion of @t10n producdon rechnology lo creale llle awarene$ ado.8 lhe

fmiogconft unity for CLcv naDasenent.

. Denonsrralion ol lam Dachinery for @tton produclion

. lntuduclioo of cotton l@fcurl vi rus ics istart vdri€lics

. D€nonslratiotr of cdfton poduction teclnologt io credte llle €4&encss anotg the

faming comhunily for CLCV nmagenc.l.r DenoNtralion of fam mchinery for colton poduqion

. l.lrcduclionof couonleaf cul virusresistlnlvaricties

. Denomtation of .olton prcduction tcchnolog/ to crcate the awareDess mong tbef@ins conhunny tor clcv ndacen€nl.

. Denoalralion oa fm ndhinety for colton prcductnn

. Introduclionof cotlonleatcurlvirusdhttrlvaieties! Demonstmlion of conon ploduclio. lecbnoloey lo crcdle lhc awareness mo.a the

fdnins oon'n'u.ity for CICV nanag€nr€nt.

. D€donslration of iam nachioe.y for couon pioducdon

. MoE cultivable land of Balochistar NiU cone uder cotlon cuhivadon, which Rould

help in disine sta.ddd ofliving oliuial populato..

. Oldalion oa lhe rojei shall btoaden $e spbere oa A3icultue Exte.sion sraff $mu3h

trainins loh f€llow scientist md olher i€source pereN lo lackle cl-cv di*de

. Cnhivation ofcoron in Nsirobsd ffiibry Rill e!. tne eea ftom fic problen ofwaler



AGRICULTURE EX]ENSION DEPARTMENT, NWFP

Eeh yqr afton sced of cLCv Esista.l vdiclics will be supplied to fie sroNes ofDalochist n al s0 p.rcen( sobidy in $e nrsl ye&. 40 percenl subsidy in scond yee, 30percent subsidy in lnnd ye& dd 20 Derc€nl subsidy in tburlh yeu. Aedcullual dachinery

will als b€ prcvided on the sde subsidy levels s sceds. NWFP govern'ncnt will set up a

ommtue under Dimlor Cene6l Agricultural Extension lo frme rules adrcgnlaliondcnreiia loreligidily lo subsidiz\t seedtmachnEry

lhe debils ofNWFP Coton pogranme {e as follows:

1'.rYe.r ('t3r D€c.mb6r 2OO2 to 30$ Jun6, 2oo3). Inlroductionofcottonleaf curl virusrcsistanrrarielies. DenonslEtio. of colton production technolosJ ro creatc thc ,sareness anone thc

faminscomunity for CLcv nanagenent

. Demonslratio. of fon nachihdy for conon prcduclion

. Ihtrcductioholcolton lcafdurl vi6rcsnhntvarielies

' Dedonsrdtion of cotton Foduclion lechnology lo creare lhe dwarcness aDo.g lhe

faming onnunny for CLCV mmagemenl,

t Demonstration of fam nachin€ry for coltoh psduclion

. Inlioduclionof cotlonlealdud lnusEsislanlraielies

. Denonstralion of couon prcduction teclDologv to creaie tbe awreness among thefaming communily for CLCV nanasement.

. D€morclratio. of fam nachincry for cotlon poducrion

. lnrioducliodof cononl@acuil virusFsistlntvarielies

. Denonslration of corton prcduclion lcchnology to create the ludeness anong the

fami.s conmunity for CLCV na.agcnenl.. Denonsrration olfam nachi.ery lbr coLron prcduction

Output ofth. Prcject. Tlie virus tue cotlon cultildtion NiU be ensured.

. Help in achievins sovernm.nfs obj€clive olconbatins the present CLCUV silualion.

. To help the Covemenf s in Poleny Allerialion.

. w'{.r srg€ vill be rdr*d.

. Fmiog co@uily socio-esononic stalu will b€ uplincdr Encouse privat€ seclor.



PUBLICATIONS

Exrension wort€rs fld €ducaled farjners are nrore inlerested in sbon, elsy to undeNt3nd

Dublicaliotr. Knowledge about existi.g iechnologies and ihfornation lhat vould be

se.eraled nom $ienlilic eseaoh lindinss will b€ convened into ertehsion infoination md

sunable nessges to be lmsnnted in lhe foih of bullctiN. btociur€s, rcmmmcndaro.s,leaflers, €tc. ?rinred naterial will be p€lerably pteF€d in local/natio.al languge

AV MATERIAL

Audiocssrtes end viddo deme.trietprognninrs aboul tho oh-gding lield activilies will

b€ prcdued and will Fach the famen lhrcugh Rldio Pakisrdn,nd PTv.

USE OF MASS MEDIA

Prinl ed elsrronic nedia wuld be exLensirely used 1o delirer the linal messages" lo tlE

end use6. This would include dre publicarion ofpopula soience arlicles in natioml mdi€sional lmsuses md brcadcG/leleqst of audio-visul prograne by Radio PalGlan md

PTv. Radio md TV hlks by €xpens would also be orsdized as a reeuld activny alo.g with

on-sirediscusion pbslmes wilh panicipalion by lbe scie.tists ad limers.

WEBSITE DEVELOPMENT

Alllhe ext€nsion mtctial publishcd duing lhc aclilitics oflhis pioFct will also be mdeavailable on InGdet. For ihis purpose specill Urdu le6ion of Website will be developed.

Audio Poerus od Video docunenleies will also be Dade availabl€ wiln play, te@rd &d

ADJUSTMENT IN WORX PLAN

The abore wotk ph ha b€en tlanned on tne rsstrtuption ot alailabilS of adequale funds

for vdious acrivnies poposcd in lh€ docunenr. In case dainadequrre lunding, the prosam

can be rc-pdoritisd 6nd $aled down to nalch the rlailabilitt of ftrnds

IRAINING PROGRAM

'1 he rraining progrdure will be laucned for scientkls and technical srafl al larious fedeEl

and prcvincial Bearcb iNtilulio.s lo keep abEa$ wnh lhe latest technologics i. thc i€lat€d

discipli.es. Comnuiq, padicipalioh a.d Eprcscnlalio. will bc aPprecialed in wortshop,exhibiliondd lielddemo.stralions.

PROVISION OF FUNDS

Altempl will be nade to lrovide fuMs lo provnrial acricultual depanments lluousb the

su6'omc6 of ACPR dl Povi.cisl headquad€b for thc purpose. rhe NIS for ilenized

expendituF for eoch instilulion will be prepared by lhe Projccl Dtector ,nd rerlected in the

budsel book each year. In cde lhat was nol possible, ihe budect will be prcrided drcu8lrtmsfer glmr. In lhat case, the povnrcial Agricultural Depannenls wiu op€n bsk dcouts



to opeEle the bdgel. Altendlely. the previucs may $e sny ott€r sysl€s for $. pup*dult approved by the Provinci.l Gove.me.ls lo hedle lbe pruvincial accounts.

Thc PCCC is a aulono@G orgasiati.u md d.cs ml opc$le ituough AGPR rrsren hwill .ot b€ possible to @fl.cl ils itenizcd cost ttuouah NIS again$ ACPR, Islmbad. the

funds for PCCC and PAEC vill b. E0ecred in fte budeet ol Fedcral Coordinarion Uni! oIM|NFAL uder lhe hedd lrdsfer gdnls. Th€se srants will be rletued to PCCC md PAEC

throusi aCPR, lslamabad To opeEt€ these iundi. PCCC sillopen Bmk accounr or use ilseaBlins s)sim 10 chMelise 1be fuds.

FINANCIAL DISCIPLINE

Th€ fmds will be provi&d a a Ir€de6l Ennt to tlE panicipati.e inslilulions outtimd ind€ PCl. Oo teriimtion of the Doje{ rhe drts will sland t dsf€ned lo th€

panicipsling instirulions The MINIAL will not entcrlaia my inegule/uaulhdzednpenditue. MINTAL

silalso nol pick ay lilbilitis alter closu.€ oflbe projed.

MinisFy oflood, Aericulrm a.d Livett@t will conduct .Mual rudit and thc exl€rnal

ddit *itL be ffied o$ by (ne Fedeni Aldir Depann1.

The provision oI lirnds lo sy componed prcgEn in lhh PC-l will bc co.dilional ongood perfommce, TIE fudds lo slow noving or not good petfornins mdlocn(s will be

The level of subsidy on inpnls will be adiusted lo malch lbe availabilily of ftnds.

llowcver n wil not be noF lhan thc proposed subsidy h ths PC- | docme.lf$vnrcial Cov€rnoed will follow lhe prccedures laid do*n rn rl'c prcvincial purcha*

innnual or $dding hslnctions for effccli4 purchases

lCCc wiu follow PCCC dles lb! allccdrs pudhdcsThe lwo vehicles of la@r CLCV Pioiecl l99s in us€ ln MINFAL Nill b€ mnlcnedfor ue by lhe Pbjed Dtecbr of Fedeial Coordiratun Unil (Fcuyceilnl Cotlon

Resdch Insdtute, Mullm.



APPOII{TMEI{T OF PROJECT DIRECTORS

Conon C.mhissioner/DiNior. Cd{Rl Coltq\ Rescarcn nsrnue, Mnhan qill ,olk is NdionalPrcject Direcror h.adine Federat Cdrdinalion Utrir ro be l@ared dr Mulr pmjed Dnecror forvdnous conponenB will b. as rollo*s:

.DiEcror

CentilCtunReseq.h

h{n$q M $' Dir*lor, cellsl co.lo' Rearch Instnde. satr.N. Dircror,Nucl.arlnnnuleof AAricuhure & Aiotoe/, Faistabadt Dn*r, NarioAll ln$ntr fq Bidehnolosl & Ceneric Ereineeriig.

r Dir*ror, coro, Ress&h rndirore, Fanalibad. oiactd ctnebl A8rilDlrse (8 . & Adaplrve Ressrch) I'u'rjab! cofron Botanis! coton Rsearch srarion,lsrdojan. Dtecior Cener. I Aeric ulruE Exrension, Querh, Bllochhranr D'e'icr offe' A3'iculruE. Ac,trulrrR tvrn.'o. D.t t(ir,

MONITORING OF PROJECT

Agriculturrl Develdpm€ni Con,nisiotrs (ADC) witt b€ rhe llariotrat Prciecl Coordinaror and witlmonirorrho prcje.. MlionalPrcjecr DiErodnd cortoD conDksioner, MTNFAL wilt !t$ nrniror.hd prcjcr on r.gular bais, nr}' rill b. pFvidd t rspon, POL, conrpured lid oltDr fs.ifiie

A reDior ldal *pen wil evie* ltFpurpos *ilhi', fBhe*ork oardes atrd

Pojet during 2005. Lel s$shad *ill br hjnd fr rhis

Pd.ntrtiotr rnd Sc.uliny oltne PojectA ff*fi.1ion m thc Fojeot was nade by ,\DC (Dr Nluhamn$d Hado ro I odmifree ot MINIAL,Pldni"e, Finmce rnd Pakisbn Arornic Enerly Con ission Ih.r mer utrder de Chainnrnship ofs6rcr..y (F&A). Tle mnftjfi* &r up. sub,commiree ro {rdii'ri& the iren iad .xpendittrre oaihe

r. Dr r(.us A.Mirik M.mb.r, Bidrosi€l srflaq patrc, rslanigad c|[imm2 Dr Muh,hmad H,nti As,icu NFAL, khftbd M.mbrtr. Dr YahF ahd D.pry chjer (^!rh u r'ure) piam ing D i is ion. h tanabid

Thesub,comnitie.netth.slmedayondsourini4drilcxpendirur*.frheprcject.Thepnjecrwasrcvhedinthelighlofexrminelionoftescniirycom uee.The prcject was subn,ted 10 secrerrry MINFAL*ho funher ser up a sut'-co[bi e.loscruliniz.lhis

Addndrr s4rehr Agrlerhft. MINFA!

Diredor. cednl c.tud R.erclt lnaide, MULGn

The $b smmiuee meeting rvrs held.i 20 tl-2002 a0d rhc jtojccr Ms scruririad *ft rsped loal/ its EquiEmeib aid modiried.

rhe prcject *rs apprcved in 6eCDWP neering heid oi 07,07-2003 subjccr ro mrionetib co{ ofnrequip'netrt in coisulErlon with I'laniing & Dovelopme[ Division I'r adcodance ro rhedecisjod ofrh. CDWP m€rir& rhe cos of rhe polecr *as Hrionalird in tr Nerilg held on 26,0&2001 sdqlh. Chaimrnship of M6be(SD)/Senior Chiea ( &F) of Plnniry & D.v.iopftenr Diviston,



AENEFITS OFTHE PROJECT

The bercils oflhe !rol..1 o€ e^reLlsd !s follors.

. r h. prcjed rill hdp ro dev.lop Bscv abbd cotun raii.ri.s'o

susaii qop prodldioi.1ri.prcjd*illbiigodapr.kq.ott.hnoloe/ron,nimiz.rheloses6sedbyclcvdisas.

. It€gstietdp.r ri( ldddarill inqre. Thc @ony or ie counry *ill Fmin ;bble.

. rh b.ch and.ndsion &riviti4 will be inr$red dd bketr odr

irxrirucs buildins to ri. f'elds onh. ftmns.. Jh. esrcn .rp,bilit orleeh iniirlriod *ill b. improv.d. Tlr. vorrinS crp&ig and $i.nrifi. I'nowl.dse orrhe &i.nrht w,ll b. inproved.

iniicr. denoiirarions ,nd orrFr

! Cotun pmdudiotr l0 dillion balor and Pak enony 9225 Uss n,illion vill b. $sbin d/incEA.d.

12. AIR.ANGEMENTS FOR MONITORINC A]tD DVALUATION OF THEPROJECT DURINC TMPLEMENTATTON

Sleering Comdill.e:

PtuvincialGovemmertsofPu[abffd Sindh sillse! upa SlerinEComhifreeas follors:. Addniond Chiefsecabry Prannins& De'cloptrMrt Sdr.bi' Agriculture. Dnedor Ceieral Agridultunl Rtsarch. oid$ ccffi.l AeirultrEl Eilcmiurt ChiclAS.icllrurc,(l&D)DeparhenrI ArepEntrtivefio'nlhelinaiceDepdnrcir. Agricultuml D.velopn'ert Comnissione/Colroi ConDissioner(MlNlAl-i

TerhsofRerer€rce. The comniftee will b€ responsible Io 6sure irregral otr dftht Resarcl' and Exl.Nion.divnies al fdmer's fi.lds ar rh. Ininuiotrs

. Tho prccmm tons so@rhly a rhe achievenenh of rhe rarecls df rlE projEt will be

monitoredandremediolndsuesl enfof pbblemsolving.

Sleri.g Cohditte ro. f€dehl Inrtilulions

t AsriculrunlDevelopmentcommhsioner ChannFn. Didcror, CCN, Mnlran Secrerary/Membe!. Dneb.. ccRI, sikmd Membei

t Diector. NIAB, Fais.lrbrd Membc'

r D'fl ro', NIBGE, rai.al,hrJ vc'nb.T.ms of refeEne will b€ sme 6s fort.ovincialneeri'gcdmninee.

13. CIVE DATE WHEN CAPITAL EXPENDITURX ISTIMATES WEREPREPA|ID: IF PRIPARED MORE TIIAN oNE YEAR ACOI CONFTRM rFTIIEY ARE STILL VALID



0.095 0.t91 o 692,/

6.300 r.875 0 000 0 000 3175,/

Machnery and Equipn.nt 9.450 13.743 6.200 32 883 P

0.050 0.200 0.7i9 /

0.520 3605 3095 2.945 10165r'

12 317 39 623 24 319 24.052 96431

2a,792r' 59,441 34,073 26,739 149.045

,-

14. GTVE SUMMARY OF CAPITAL COST, COVERINC'THE TNIIOL' OS'fHEINVESTMDNT PERIOD AS INDICATED BELOW: aRs, Milliotr)

ls. BASIS OF COST ESTIVATf,S: (CIvr rULL DE I AILS)

The estmaies de bsed on oFn ndler prices.

16, TINANCIAI PI,ANS:

F€d.ral Covemrenl will provide the funds. Dcrails of the establisbnent &

opeiatiodlcost

fgiven in thc Amexur€

r7. BENEFICIARYPARTICIPATION:

The be.etcidies will be fame6 of aU the lor provinces of ?alist r. Tbe fed€nl

a.d pmvincial insliluliohs *pacily r ll !e inrproved in reas of b@ding,

ott4eneli€s, biotechnologj/, g.netic eneineeing, enlonology, palholog'/vilology,

thysiolosy,.gmnony, and ttenc. o|tecnnology

18. RISK ANALYSIS:

19. ESTIMATES OI ANNUAL RDCURRING EXIENDITUR.E Af'TERCOMPLETION OF EACII PIIASE: (ALSO aNDICATE THE SOURCES OF

FINANCING RECURRINC EXPENDITURE):



t20. AUTHORITIDS RESPONSIBLE TO MEET TUI RECURRING

EXPDNDITURE AFIER CoMPLETTON OI THE PROJECTT

21. UNIT COST FOR EACH CATEGORY OF SERVICE OR OUTPUTIINDICATE NUMBER OF TECTINICAL TERSONNEI AND MAN YI]ARS,NUMBER OF STUDIES, COST FER STUDY AND COST PER MAN YEAR:

22. COMTETITVE UNIT COST OT SIMILAR PROJECfS UNDEITIMPI,EMENTATION AND COMILETED:

23. GIVD STATEMDNT SUOWINC PTIASINC OF PAYMENT OI' LOANSINDICATED DEBET SERVICING CAPACITY:

24. INCASE OF INDUSTRIAL RI]SEARCII, IF TgE PROCESSES DEVELOPED

AR.E TO BE LEASED TO COMMERCIAL FIRMS: (GIVE CASH rLOWSTATEMENT INFLOW AND OUTFLOW) FOR THE FIVE YDARS

25. ANNUAL PIIASING OF OUTRDACII PROCR{M AND FINANCIAL

RDQUIRIMDNTS FOR ItlE PROJECT: (ATTACH PDRT OR DARDTAGRAMS IF PR.DPARED)Oilen in Ameiw,



PART'C"PROJECT REQUIREMENTS

CONSIILI ANTS

,) Tne follori.g sr.ff *ill be beed lor FedeEl coordiDlaiotr Unic

iLh! of t1F Psi No of posts BPSAc.ounls officer 1 17

Assida.t conput6. Progdmtref 1 16

AericultuEl DelelopDe CoDnissior.r *iil lolk a Natianal PsjarCNrdin.ror DiEror, Cenral Co[o' R.search I'Nlnure, Mu]tan *iil work as

All techni$i mr'po'er reqljred for rhe prqecr dcriviGs wo+ld t. n4 ht rhc

erhring sraflof lhe iNlnu ons olfedcdl6 Roll cs prcvinces involved h lhd

fie Federal C@rdinarion Urit (fcu nalIrill be rc(uited only fo{ {€ Prile.t

Thr P'oid Dndor G) 'iltbe Eiven appropri'le I'oiorari! rirhin rh. provhioi

of Covernhenl rules ord reBulalions.

(b)

G)

Gire tirl ofeDploytoenl lo be geder.ted 6y gebder.

Due to Folicies of $e Prbent Co'!. ire exislins slati would !e utilised in ahis

Give d.trBower requircd dqring {h. firsi Wrr ol lhs inpl.m.trt.riot olrne p.oj$a. Giv€ dct.ilx of rFcific arJll! req{ired Gcientbn, tcb/liddrorkeF' rechDici.n cr) sq,ant€ly ror nale lDd febate anJ lb€ir er.d.s,

Ukety s[ort g€ of $lnDovq by o.NpBrion,

SteF to b€ alka to !$ure,vril.bilitt of mrtrpo*ei

The extting stafin th€ resetrcl synen is radily av.ileble,

(d)



(I) Approidri. rudbo ofpeBors r.quire.t ao be blin.d per ycrr (tocdlymd abhrd) &d (h. kind of stiilh to be rcarnr

27, CtV[ WORKS

28, PHYSTCALANDOTtlERFACILTTiES R0QUiREDtORTITEpROJECT

29, MATERIALS, SUPPI,IES AND EQUIPMDNT RIQUIRXMENT

Derail.din

dexLte30. IN THE CASE OF [I!TPROV!]D MATERIAL AND T,QUIPMEN'f FOR

EXECUTION,INDICATE

a) Jstifialio! aor inponb) Prcposed eurce/surcar ofsupply



o

FEDERAL INSTITUTIONS

L CEr|TRAL COTTON RESEARCH INSTTUTE. MUITAN

LrsT oF EQUIPhET{TS

Adjusi.ble Speed Shaler

2. Air-@nditioner Split lype (2 Ione) " t2 0.600

3. I 0.o€o

Conoul€. P4. Lasor 8 Coror Pnnler and S€n.ere(c.

5. I o.100

6. 0.500

7. Srirl Can6'a & adesrones (setof lec.s)Pholo@pier wilh acessoried 0 600

0. 0.050

Diqialvid6o c.heE dth a@6sGsEarth Themometd. 20.30.60,110$m 0020

12. 0.030

13. a 200

Farh !la.t\i@rr, Rid!€rrh+larn.r, B6d shaperCuliivatorlillel Pl5nke., Ctumbref. Chdel Maks, Oi$H.mw, aeit Furov Weedins Equipmenl, conc€ieMirlue ior s6ed delintins, wieat dnll Chilel, Rolavaror,

Higb clesrs€ a(a6hnst

0 650

15. 1.400

Gimrng Machi.6 (bo-S&v) Conplere 0 t00

HsidifDr vilh {hetnos1al 2 0 r00

1B LaD lop conpuler {Pentiuh-M complete with I 0.150

19. Bft r'ng/La'@tion M;cnkE 0 050

20. o.250

21. MieosaP€ w{thosl carera 2 0.200

22. 0 013

23 2 0 060

llvliiCla@t Mlso+rp€tle I 0 030

25 Multim€dla q6td silh a@Esoies & e{ra lrmps 0.250



26.

27. Phot@rnlhes€ lr\6ui.o Eys1efr

Pdr{er ls Chemids 4d Tefinal PaPs 0.200

29.

30. Record'nq 6vrpor5l'on b.l6e 0 030

31. 0 20032. soir sanp,ins rub* wilh Hamnl4g6!9!19!!33. Spray€.s (5 Mororized + 15 Hand) 20 0 t50

0.0s0

Trador hountgd boom sp.ayer plu. ,c.essones 2 0150

33. 0.500

v\Jind -lelFmel€ring Device,

indi€tor & ior wihd v6locity

1 0 080

1

42. PVC Yel,ow Traps ro. whileny 5000

43 1

8.953

-h6 €Fcr pd@3 sll be rMn ar the hmen Th€ p@*nl Bir sod(6nio sYstam

upoEdFlio. is tul Po*ible h3r'6 rhv the

b.!ts ,hicn !i[ esun h .ne4v .aMng.

lri lhe latd.rbn* is very old. lls rcluvenalion ,ndspll unii. wlrbe insral/ed. The un



CEI{TRAL COTTOI{ RESEARCH II{STITUTE/ MULTAT

ustoF Gtassw RE

Si

2. 0 015

3. aeak€c. Boro.illi€te (v{ious €pacines) 500

Bu.ed€s. Glas6(variousepacitie.) 50 0.020

5. 00t0

6. Crucibl6. (cer5nics) with I'd IO 0 010

cyrindeB. Graduated (va 0u. capaciries) 0.020

s obD6able Potproprlene tbes 15 nr 50 rdr 0.100

9. Dudn laboratoryBotles {100, 250, 500, 1000n|) 0.050

t0.

F l€r papd ( 12.5 6 dia ) 0015

12 Flask, Polyca.bon.le (vsnous c.pac ies) r00 0.030

13. Flasl<, Pyrex, Kjeld.hr (various 6pacit€s)

Fla6k, Voumetic (vadols cspaciti€s) 0.0f,0

15. 25 0010

16 clasr Bealer {200 ml) Py/ox 20

17. Glasr bonle wilh lid (5.lOdl) 50 00r5

18. Glrss Jsr (2.5, 10 rbs capacity) r00 0.005

Ldev Surgi€l Glove (ldedium & L*ge)

20. Olher Gla3.ware/Plastisare 0100

21 200 0.150

22. Mdo@ntriflse rubes (0 1 0 2. 1 5 mr)

0.015

Pet dish$ {vaaou. 6ize5) disodablo1000

001025. PipetleTip€110,250, 1000u0Whit6.bhe&yerlow) 30.000 0.050

26. 3 0 030

27. Ieel'rub$ (v.no66p5ctues giastPl.si'() 500 0.010

23 1000 0 009

29. Per dishe6 (Py€r) venous sizes 200 0 060



CENTRAL COTTON RESEARCH INSTITUTE. I'IULTAN

usroFchEMrcAtS

Si

1. 259 0 007

2 0.1€o

3. 8x50Oml o 060

0 025

5. 3XI tit 0.021

6 4r5009 0 090

7. AOar As* (Emerck, Gemany) 5k9 0 0rt5a 3r5009 0 350

I10. Amofla lobiion (Analat 0010

Ammonilm a@tal€ (Analar) 3x5009 0 010

12. Amfr onium m€tavanadate (An./ar) 215009 0 011

I3 Ammon un nolybdar€ (Analar) 11500 g 0 020

A.xmony polaesium (+) tsrlGle (Analar)

15. 2)1009 0 0t2

2009 0010

17 0 002

18 3(2509 0 t75

19.

2A 1og 0.002

21 1'25 q

22. 25s

0 008

24 0 004

0 008

26, cabium hidroxide (Aturst 2\5oo g 0.002

27. Cardum svlphare d h yrdate 3x500 g 0.005

0 006

29. 0 020

30.



rl31

0.050

2t5AA I a 002

34. Conjusate Adi molse in Rebbit lgc(whole 0 050

36 0 002

CopPer 6ulPhal€ (Anala4 2\500 g

33 13,500 q 0 320

2^5s. 0 002

40 0 061

lK9 0.100

42 Diethvdithi@tbamat.(Sodiuh

sa!l) NaD

ECA 20090 050

15^250 !9 0 930

44. dNTP SEi (dATP dCTP.dTTP dGTP) I 025

215009

0 062

48. 0 002

5x5g 0.065

50. ElhYene oiamins lea6a@lab 0 009

51 Ethylene diamine l€tra a@ti6 acid dr sodum 5/500 g 0 007

o072

o 024

v F6rou6 sulphale (Analar) 2/500 ! 0.004

200 g

5"25 g 0 075

53. Gas€s (Ac€tylene, Nitrous or d6, Algon, Helium,

Nitrog€n, Hydrogen)

0.050

59 0.050

109

Glu6 (for yellow sticky tdps) 200 kg 0.200

62. 0025

63. Hyd@chroric rcid (Anarar) 3x2 5lll 0 003

3 o0a5

65



66.

69. r0og 0 006

70. M.gnesium (100Ous/mr) 0 002

71 Magneriun n rare (AnEl.4 0 002

72. Magnesium ehhaio {Analat

Mansane* (l000ug/mi) 0.002

0 020

0 005

lx2 5l 0012

77. 0 002

74. Melhyl Pafab.. (melhyl-4-hydrobo.zoate)7S Melhyl rcd (pH indioalot 0.003

Monoclonal anlibodies SCRI 4 52 54.60 5 0 500

a1 0016

a2 0 002

002s

84. 0 005

35 1v2 5 [9 0 004

36.PeEhlor ic .cid 60% (anala4 5r2 5 ft 0 030

33

a9 PhenoFinhabin (pH indi€lor) 2\ 1009 0 0ol

Phosphelg 6ub.t6l6 lablei.

9I Phosphorus ( I000 ughl) 0.002

92. Phologene 3yllem 2.0.nd bionic raberling syslem 0 300

9J 200 g 0033

Polyerhylene Gltcor (PEGI 3000 0 008

95 500 9

96 Potassum dichrcnel3 (analar) 2r5O0g 0003

97 Polassum pemansrnare (Ana.r) 1,500 s 0 002

08 Plolein Analysis Klts (Strlps. Eri6clors Butrer elc )

t00 0.200

IOI



102. 0.073

103 r, r00 g 0 003

509 0 010

l05. 1r I00I 0012

106. SodNm .@tale anhydrous (An.la4 0.015

107. sodium hydroxide perlela (A.alai) 10'519

104. 59 0013

109. 0 006

1t0. stcprmycino sulphale s3501 311009 0.028

111 10^2.5tt

112 T.q Dr,rA Pdyme€e6 lilh b!fterc 55x250 I 400

113. 500 u oi22Tet€methylelhy'mBdimire 0 0t8

115. 00J3

116. 0 003

117. Tds(HCl) 24.5009 0 630

118. 1 00tl119

124. 3Kg 0 013

121 0 015

122 3r I0g 0016

123. 0 002

,11.251

! Th. .kr prirt! rill 6. knon d n\e



CENTRAL COTTOT RESEARCH TNSIITUTE, MUI"TAN

Alllrual PHASIi{G oF ftr.tA$cIAL REQUTREhENTS OF TltE PRO]ECT

PhologEph(Msi'l.PhotolEphy



ofr6 .quipn.nb (@nFr.L Dhtupi6r

csiEdtud Gkirr.d, fskir.d) etor6

€rea'iflait r inp,b (.€€d, renllz.r,



A46nmabbl,|@Edgd:2600lqnlkmdq|.sd|heR5@{t

@b b,t'cod ir !yn@ vdidm sFr4

t Lebd bb b tu m* Fd4 d tr{url.mna, dns Gs 'd

bMr Fei!,'..lry!4m4@silddhodsb,@.!ve:'d€!n'm

6rd! 6d ddtoiy (tu dat €dr

ji)Ms!E'rgah|fuidd.e'nmhDry

Fbrd Enid3 ,00 cc DFler (r,

c@eter 2€.s€rar tor lamets rDinins (r)

T6dor 35 HP(ccRr MuLr.n) (r)

rr.er60NP(ccFr Mtui)()

16.120 22.922 12.891 LO.A9 62.rs21



t

2. CEI{TRALCOTTO$ RESEARCH t$SrrrurE. SAKAAND

A) LISTOF EQUTPMENTS

Ar Cond loneG (Iw un(sof 1.5 tons and hro or 2 0lons) 2

Rerr seEio6 (one tun s'zeandone medium)

O*p lGeze6 (-40 and 69C) 2

comp0t6E (Pc Peir umrv with Le

Video CamoG wilh .c$*t3PholoeLi Mach'ne wih €duced and €iErge runcrons

TOIAL 1.355



a

2. CENTRAL COITON RESEARCH TNSTTTUTEI SAKRAND

ANNUAIPHASING OF PROJECT PROPOSAL

RECURRING

Publiciry a nd Adved $ment

Pr nrng, Pholdg€phy, R€pon

cont€ctuar seryic6s (sklted,

I{OI{ RECURRIITG

R€h.biritd d or Laboratod€s

1.355

1,555GRAND TOIAI o.720 3.160 1.400 1.205 6.445



Annar!B{r{r)

3, NTCLEAR INSTITUTE FOR AGRTCULTURE & BTOLOGY (NIAB),FAISALABAD

LIST OF EQUIPMENTS

oigital Pl.nt Canop! lma$er

2.

Pod.bl. Phoiosynth*b ryslem

5

Elect'onic & 5n.. lc.llenr€mp)

3, TDR Sgnsor ls so'l wale' nanasement

fusjdudrneG25o*t6o Bie drs drnir d 4 i

hdd.vabofuhdl'gEiedll.stsEb'hdhc6d4 rrh, €iftdri.,i!D drrloid



Annqure-Irt (b)

3. llucIEAR INSTITITTE FOR AGRICUI'URE & BIOLOGY (NIAE),fAISAI.AEAD

AIIIIUAI PHASII{G OF PROJECT PROPOSAI.

conri*tual L€hou. (sktred,

2 Erp€riflslal op.Etionar cosr

5. cftsing Maie al, clo(h

Ea.lhs .nd Pberic @rc. non

slalirrry, Pnnting rftlerialand

9. Repat nainlemn@ ol €quipoenr /

woAshops / SedinaE aor tEininqot screntsts .nd hd€6 End

GRAND TOTAI L3102.841 2.530

t.?109.490



Anmxub-lv la)

4, I{ATTOI{AI INSTITUTE FOR BTOTECITNOLOGY & GENETICEI{GINEERII{G (I{IEGE), FASAIAIAD

A) LIST OF EQUIPMEI{T

5i

upgr.dalion of pLnl l'esue currue E./'i'ee

2

5. Gel ele.topho'd.'e ryrlems

Equipmeil or AFLP ersien)(.6y llm .assorles for .ubEdi€€phy

2.000



Annrxurelv (b)

4. IIATIONAL ITSIrIUTE FOR AIOTECHI{OTOGY & GENENCENGII{E€RrNG (irIB6E),

'AISAI"AEAD

a) ustoFcHEurcals

5

Ger Maxi Prasdid isoEboi ktr



tg

MuEsh'q 3, srooq media w h A5 v Edin

2t

519

cuslcm gene synlhesid$qu€icinq

Tn€.gent rbr RNA enEdion

5r9

R€genls tor pbiem aârysF

TOiAr. 13.600



OAnnexure-Mc)

4. ATIO AL INSTI'UTE FOR BIOTECHIIOLOGY & GENETICENGINEERING (NIB6E), FAISAIABAD

') LIST OF GLAsSWARE

. th. M pric* *iU b. knoM ar $e time ofplr.irs d'nd

Dspoebl€ pF€ne rps (r.r0 uD

D sp@ble pip€t. iips (20.200 uD

oispG.br. prpeha r|p3 tr00 10oo ul)

O6po.able peti dishes (90 mn ) 150 mm) 0 500oispos.b € pipene r pt (5 hD

0 3posable piee@ rjp. (5 c )

DBpeble centituso tub€s (s0 ml)

o'sp@ble enffuge hrb€s ( 15 mD

Gla* Plales (va' abl€ slz€s)

Pld bEnd media bon|* ryairbls 3 zet)

Polyprcphyr€n€ b€aker(Vadableeiz.s)

Oispos.bl€ 15 ler (0 2s ul) I nllEr aMbry

PyEr Gl$s eir,itug.bre tub€ (30 ml)

Pyd G $3 cenritug.brc rub. (15 m )

3,Oolt



aAm€xne-rv (d)

4. !4|!fr rNsfrTurE FoR BTOTECHT{OLOGY & GENETTCEITGII{EERING (NIBGE), FAISALABAD

ANNUAI PHASIITG OF PROJECT PROP]OSAL

conratu.r Manporer Fi6rd

5 Slaboiery pnhtjng datonar .hd

R6p.tr / mainieiance of

GRAND TOIAL o.Agro 16,225 3.025 21,775



a

s.l conon [email protected] In6titur.. F.r..r.bad51 Cotbn R*.rch Inslitub Farsalabed sEiiorc at Mu[.n. veh.n.

Bahaetpdr, Rahimya. &.h, Khan€Gt. Ttlrua Gumani Kol chuta. H5@nabad, Kha.Pur Jh.nd sa4odha Pipran and Raiwjnd.

5 2 Enlomorogel Reseroh S€cltdn. AAR|, Fa sarabad.5 3 P a.r Virclogy S€ctron, A,ARI, Fa'sarabad

s.r c6n6n Rd€.rcn h3ftd.. Far3.hb.d .nd it Rd€adh stadon6

ust of EQUTPUE|{iS

PROWNCIAL II{STITUTIONS

PUNIAB

Disc Hadow (Jhaig .nd Khan4a0

2 Cdlndlg HP4500 Faielabad. itull:n, vehr & Rahtnyar

Chillins cabin.l ior gemplasm (Fais.rabad)

vheo came€ with acc*soies

5. Lamd d @hlne {Falsslaoad)

Cu tivalrr 1 t hns R.h myar Kh$ a.d Khanerel

Far* ce'r'no Technology L.b (Mubi &F.srabad)

t0. FibE Lab. At condllioner Sprii rype 2 i.n (Fan hbad A

God€n Pump(Bahe.lpun

't2. H.nd knap6ftk sp€ys lor diffi*nt E3*rch {ari..e

Lifr Pump wi$ peter dixel Enqine (moveabl6), Fa6abbadRah mya Kh5h, S.hiMl & Mun5n (one bl €.ch)

2

Mec,ra.@ Conon Bl.nds CS-4s



aMl@ .pclcatoE wifl s/nng€ {aubma€)

ftrorocoDi.r bEchrtu (Mulkn & FairFtrbadr

Po€ S06y* riy drfriss\t l!sc5 sf.tos

20. Rotleard 12 3r.de. (lGrnsa0

21. T€@r Df,En T.roh:li (Mulbn)

TEctr Hi$ MF-37s (Multan)

[email protected] Tu'bine % Cu.'d Oeh$qE ( {urt3n I 3

ULV SpEv t { dm€Ent le*rch sbdons

uPs fd Pc Muh.. & Fa'sr.bad one ror e.cn

29.

Mulinedis Prci€cior (nrdus)

rod (Equlpn6r) crt, F.iel.bad



c6ron R€.e.Eh hnitub. l.€.t.b.d ,nd iE RG..rch sr.rions

3AP aenzyle Ahino Puni.5 gm

BEA6 E€do e adenine 5 9m

Nephlh.lii€ .cera.od 2s sm

12

smoph.nol bluc (M6l]\) 25 sm

102

20.

u



aA.nuu6-v (c)

5.1 cotron R*.rEh lBtrtlts, Frl*r.bad.nd tt! R....rch Sraton.

LI5T OF GI.A5SWARE

2

3.

5

Grass b.nl6 sEdoared and s.l€Mbre 100m

Gbs bonbs qEdu.Fd and *@abre 250mr

Grass botes gEdusred and s@abre 500 ml 50

T€sl tub* (Pye.) 13rl50mm

re$ tub$ (PyE 2 5\20cn

15.

20.

22

Pip€isEnd rr!€25'35(*dy)spEy bonr$ 65oml 0ng9e4

25.

27

w-h bours r 2smr (12 0&k) sci.nce MG

D q'lar €p.thve d sp6ns$ (chedp€ti6)



a

coton R€...6h hsritrt , Fais.r.b.d ,nd li.V.h.ri, S.hrut, Bahawstpur, KhrnMr, Kots.4pdh., Raisind, Khan pu r and Piphn,

Rd..rch sr.llons, .r iru[.n, R.himy.r Kh.n.chlna, rhan Gumanj, H:Eon.trd, Jh.hg,

PuEh*.otDu6bt G@ds

P0cha4 or Fumhre & F tue (otfcerabr6 .ha 6. compursr 6ks, l5b sbole)

s!b{or.r (.)(cRr, Frrr.r.b.dl

Repar ol Mach'n6r] 3 Equ pned 1.200

Reh.br Erion o, L.bodory

s€fric* Reidecd (DPr- (skned,

orher slo6 (crr€mdr. grassa6)

G6nd !or.r 1..6+ct 0t cRr. F.i..r.bad.



a

^rn*!cv(.)

'2EltIO OLOGY SECNOT, AARI. FAISALB'D

OETAIL OF EAUP ENIS

Mde C.me6 wilh .ccsdes lFa6alabadr

2. Hum'difier (Fa slabad e Sahacrpu4

srareo bin@u ar M crcspe (Mu|bn a Bah*3/puf)

5.

Fan oil h€der wift ft.m6El

Fie d hagnify nq micrcs@Pes 5

9,

chlo@phyr conr€nt Mew (oq'b )

2

tol lEqulpmo'n) Enronorogy



ann.ruE_v to

5,2 EdTOfiOLOGy SECTION. Al4lit. FATSAIBAD

ANNUAL FINAXCIAI PHASII{G OF PROJECI PROPOSAL (ENIOhOIOCY)

Purcn... ofounblo Good6

R.p.rr 3, Mtnr.nd€ or Dfibi;Cood!Rapatr or r;;F-n;pa'otl',lac'fie,y?TdFa;i--ReD.tr ol tumdie & Firtr6su}-iot t - --modni;a r sd@-

gg9

slpqggu9s

9t:9

!j!9!t!

llq

oiro

q!]!lq

!2:!

oi/o

!!!9

141

oro0

oJ6!

T6r+hon€ 3 Trunk ca r charges

SeMes @de6d DPL (Skill.d

chm@ls. Fedli6r/ peglicider/ s€.d

of)er sior* (lnsed @nng 6ck in$ctc€les b.xes 6rc.) Fal*l.bad. Mutan &

Grrd totrl of Entomology A^Rt



a

5.3 VIROLOGY SECTION. AARI. FAISALBAD

Gel €leclophor*is @mpl.l€ sy.len !i$ pMr supp y

2

3



a

5r u@!!GysEg!r9.!aB!l4sA!Ep

ActolamiddBsA.dlanida 191 6tio

5.

15

2A

25.

5

T.o DNA mlvm6Ed. w|ln bufi#



a

5,3 VIROIOGY SECTION. A,ARI. FA]SALBAD

B€lk€E (v.noue dpacliFs)

cylindoE cEduaEd (vanous €p.ci*)

Dudn Labo€bry Aoflbs (vamus €prdt6)

F a.r vorumer'ic (uanous rypes)

Gk* Bek.r (vai4, s@) Pyd

Lalex m6di€r9106 lm€d

una.d

raEe)

12. M€rsuDnq cyrindeB (10o n )

Mi@nhfr4e ubs (Erds sa€)

15.



aann.ro-v (i)

5.3 !ES!9S!SES!j!A!!4$.!AE4!EAS

ANNUAL FINANCIALPHASING

OF PROJECT PROPOSAL(PITnt

VIbIogyI

P!Eh.$ Ot DuEbr. G@d.

Purcha$ oa FuhituG A Fixluc

Fepatr or tLch rery 3 Equ pruntRehabi btion or Labo6'ory

sediF @ndeed oPL (5k ed ahd

TEv. ing.rlow5i@ POL SE$oieryea 0 350

c6nd robr {.+b+c) .r vlorogy

a.n.ru6.v(k)SUMMARY

5. CottoD R€serrct hsfitute, Fatualabid

CRI. FAISALAB,qD AND ITS RES.

ENTOMOLOGICqL RES. gECTION A^RI, r 136

PTANT VIROLOGY SECTION, AARI, 2.264

GRAI{D TOTAL OF DUI{IAB(RESEARCH)

o.451 4.796 6.423 5.054 21.124



Annex!re-Vl

6, AGRICULTURE DEPARTMENT (EXTENSION &4.R.), PUN'AB LAHORE

ANNAUL PHASB\G OF PROJECT PROPOSAI

3

5.

! MdKJrhs *ill b. Fsvld.d s hiar\nche bais It!. rsr wirl bt ft.t6d in iMlnm ad'ilr

b.d.posned Eguldy in dE Fed!61 TEaury.



a

PROVINCIAL IT{STIIUTIONS

SIXDE7. corroN RESEARCH STATIO , T NDOJAM (STXDH)

A) EQUIPMEITTS

rracnr Mounied sp€yer (r)

5

came€ {ilh tur/ E@s&e (1)

11

! Th. eed pne will bc knom d $.



COTTOII RESEARCH SIATIOI{, TATOq'AM

ANI{AUL PHASING OF PROIECT PROPOSAL

co.rr.cbrl Lbouf lski[.d,

CoM.di 6 'nd Sowles.

,.

3.544



Annexufe-Vttl

BAIOCHISTAN

A. AGRICULTUREEXTENSIONDEPARTMENT,BAIOCHISTAN

A) AIIXUAI PHASING OF PROJECT PROPOSAL

?

r,025 2,OE ,;025 t.o25

' tJ. -&tpna wiLlb.lno*i a n. umr otpl4mBd'd.A



6

N.W.F.P.

9. AGRICUITURE E(IENSION DEPARTMENT. NWfP

ANNUAI PHASING OF PROJECT FROPOSAL

Rs, Million

2

' nr. .Ed pns v,ll b. |jom d'he

M( of DLrcins o'd.n.



rah-I't

TEDERAL COORDINATION UNIT

PAY AI{D AILOWANCEE

C.nput€r Arlovane @ Rs isoor PM

Congulltnq ror rcv 6w or Piojecl

Tereohon. and Trunk crr chaG.s

5o nMre ourc hE s.rdeFloomentOlnd Ch{@ (Mi@reMl

G6nd T.t r (P.y & Allowane +Tdvelli.q AllMne + conline6.id)

o.745 r,67r 1,U4 6.012

.Twov.hic|6prccU6l''onpEviousc!cvl1995lpDieclrrcsid'r N.iron.l Pbi*r oiE.tor rtrd $. olhsr v.hi.lc m.v b.h rEti.ub, ullln

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