250�l PJ E12.5-E18.5 4) Hypoxia-PJ control: FiO2 � 12%, E15.5-18.5, gavaged daily with 250 �l glucose (13.5% w/v � glucose con-centration in PJ) Food intake and body weight of pregnant mice wererecorded daily, and placentas and fetuses were weighed at E18.5.Western blots of placental extracts were quantified for the oxidativestress marker, Hsp90, and apoptosis markers, cleaved caspase 3 andcleaved PARP.RESULTS: Compared to normoxia-FR, hypoxia significantly increasedplacental expression of Hsp90, cleaved caspase 3, and cleaved PARP.Importantly, hypoxia-PJ, compared to hypoxia-PJ control, showedsignificantly (p�0.05) reduced expression of all three markers of pla-cental injury. Although placentas from hypoxia-PJ weighed less thanhypoxia-PJ control, there were no differences in the number of nida-tions per pregnancy, fetal weights, or the placenta:fetal weight ratiosbetween these two groups.CONCLUSION: PJ limits hypoxia induced oxidative stress and apoptosisin placentas of pregnant mice. We speculate that PJ is a potentialtherapeutic agent to limit exogenous injury to human placentas. NIHRO1 HD 29190.

193 Skin autofluorescence, a marker of advancedglycation endproducts accumulation, increasesduring normal pregnancyBart Groen1, Pieter-Dirk Boekel2, Paul van den Berg1, HelenLutgers3, Douwe Mulder2, Marijke Faas4, Petronella Geelhoed-Duijvestijn5, Thera Links3, Joop Lefrandt2

1University of Groningen, University Medical Center Groningen, Obstetricsand Gynecology, Groningen, Netherlands, 2University of Groningen,University Medical Center Groningen, Internal Medicine, Groningen,Netherlands, 3University of Groningen, University Medical CenterGroningen, Endocrinology, Groningen, Netherlands, 4University ofGroningen, University Medical Center Groningen, Medical Biology,Groningen, Netherlands, 5Medical Center Haaglanden, Internal Medicine,the Hague, NetherlandsOBJECTIVE: Parity is an independent risk factor for cardiovascular(CV) disease, resulting in an increased risk for CV events in womenwith �4 children. Since pregnancy is associated with relative insulinresistance and increased glycemic and oxidative stress, the cumulativemetabolic burden of pregnancy may contribute to this increased risk.Advanced glycation endproducts (AGEs) are sugar-modified proteinsthat accumulate during normal ageing, driven by glycemic and oxi-dative stress. This process is accelerated in diabetes mellitus (DM),contributing to vascular disease. Skin autofluoresence (SAF), a vali-dated non-invasive measure of AGEs accumulation, has a strong pre-dictive value for mortality in type 2 DM. Earlier, we showed that SAFis increased in recently pre-eclamptic women but it is unclear if SAFincreases during normal pregnancy. The aim of the present study is toinvestigate SAF during normal pregnancy.STUDY DESIGN: 54 consecutive healthy pregnant women from theUMCG midwifery clinic were included. 13 women dropped out: 4moved to another clinic, 3 were unable to attend all measurementsand 6 had a complicated pregnancy. SAF was measured with the AGE-reader (DiagnOptics, Netherlands) in the 1st (T1), 2nd (T2) and 3rd(T3) trimester of gestation; in 20 women also 12-16 weeks after deliv-ery (PP). ANOVA and paired sample t-tests were used. A p-valueof �0.05 was considered as significant. Data as mean�SEM.RESULTS: 41 women (age 32�1 yrs) completed the study at term afteran uncomplicated pregnancy. SAF increased from 1.48�0.03 (T1) to1.60�0.03 AU (T3), p�0.014; SAF did not change thereafter in 20women: 1.57�0.05 (T3) to 1.57�0.05 AU (PP), p�0.938 (Figure).CONCLUSION: SAF, a marker of AGEs accumulation, increases duringnormal pregnancy. This may reflect the cumulative metabolic burdenof pregnancy and contribute to the increased CV risk associated with�4 pregnancies. Further studies should address if increase in SAF isexaggerated during pregnancies complicated by pre-eclampsiaor DM.

194 Omentin, a novel adipokine with insulin-sensitizingproperties is associated with insulin resistanceindices in normal gestationBenny Brandt1, Shali Mazaki-Tovi1, Yoav Yinon1, Eyal Schiff1,Rina Hemi1, Hannah Kanety1, Eyal Sivan1

1Sheba Medical Center, Department of Obstetrics and Gynecology,Tel-Hashomer, IsraelOBJECTIVE: Omentin, a newly identified adipokine, enhances insulinmediated glucose uptake in human adipocytes, thus inducing sys-temic insulin-sensitizing effect. The aims of this study were to deter-mine whether circulating maternal omentin levels are associated withinsulin resistance indices and to assess which compartment, maternal,fetal or placental, is the source of omentin in maternal circulation.STUDY DESIGN: Fasting serum glucose, insulin and omentin were de-termined in 25 healthy pregnant women at the third trimester, beforeand 3 days after elective cesarean section. Cord blood omentin wasmeasured in their 25 neonates. The Homeostasis Model Assessment(HOMA) was used to evaluate insulin sensitivity before and after de-livery. Non-parametric statistical methods were employed.RESULTS: Antepartum maternal omentin levels were negatively corre-lated with insulin levels (r��0.41, p�0.04) and insulin resistance(HOMA-IR; r� �0.41, p�0.03) and positively correlated with insu-lin sensitivity (HOMA-%S; r�0.4, p�0.04). Postpartum omentinlevels were negatively correlated with maternal BMI (r��0.44,p�0.02), antepartum HOMA-IR (r��0.49, p�0.01), and beta cellfunction (HOMA-%B; r��0.47, p�0.01). Median maternal omen-tin levels was comparable before and after delivery (57.2, IQR: 38.2-76.2 ng/ml vs. 53.4, 39.8-69.4 ng/ml, respectively, p�0.25) and highlycorrelated (r�0.83, p�0.001). Antepartum maternal and neonatalomentin levels did not differ significantly (fetal: 62.2, 44.3-74.2 ng/ml,p�0.77) and did not correlate (p�0.6).CONCLUSION: Circulating maternal omentin levels are correlated withinsulin resistance indices, implying that this adipokine may play a rolein metabolic adaptations of normal gestation. The strong correlationbetween anteparum and postpartum maternal omentin levels, as wellas the lack of association between maternal and neonatal omentinlevels, suggest that the placental or fetal compartments are unlikely thesource of circulating maternal omentin.

Skin autofluorescence during and afternormal pregnancy

Poster Session I Clinical Obstetrics, Epidemiology, Fetus, Medical-Surgical Complications, Neonatology, Physiology/Endocrinology, Prematurity www.AJOG.org

S92 American Journal of Obstetrics & Gynecology Supplement to JANUARY 2013