1 97 193 289 385 481 577 673 769 865 961 105711531249134514410

102030405060708090

h706 (control)

1 100 199 298 397 496 595 694 793 892 991 10901189128813870

20

40

60

80

100

120

h88 depth

10k bin

1 97 193 289 385 481 577 673 769 865 961 105711531249134514410

1020304050607080

h225 depth

10k bin

1 97 193 289 385 481 577 673 769 865 961 105711531249134514410

10

20

30

40

50

60

70

h144 depth

10k bin

Supplementary Figure 1. Comparison of genomes missing dpy-5 and/or unc-13 markers. A. The distribution of EMS mutations are plotted along the length of chromosome I. The genomes missing markers have more EMS mutation in the first 7Mbp of chromosome I when compared to a control genome (h706). B. The average read depth per 10Kbp of coding element is plotted along the length of chromosome I. The x-axis shows the coordinate in 10K units. The y-axis shows the number of reads. The control genome show 33% more reads in the first 7Mbp while the genome with missing markers shows a flat distribution.