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Your Baby is coming with a miracle gift to your

Family

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Your babys lifeline for nine monthsUmbilical Cord blood..may well be your familys life-saver against deadly diseases

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CORD BLOOD A TREASURE TROVE OF STEMCELLS

Residual blood present in the cordwhich was once a Medical Waste

Now A Medical Miracle!

Cord blood collection procedure is

no harm to CHILD and MOTHER.

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WHAT IS SO SPECIAL ABOUTUMBILICAL CORD BLOOD?

It has high concentration of specialtype of cell called STEM CELL

If the cord blood is collected at the

childs birth then these Stem Cells canbe harvested and stored under 196Ctemperature for future use

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Stem cells are master cells

Get their name from the word stem asthe stem of a plant.

Tissue precursor cells that havethe ability to self-renew anddifferentiate into more specific celltypes. Stem cells serve as acontinuous source of new cells

The goal of any stem cell therapyis to repair damaged tissue thatcant heal itself

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Stem Cell Differentiation

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Chief Sources Of

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BEST GIFT OF THE LIFE

Make sure that you offer yourchild the disease free life and

also inturn your child makesyour whole family protected

with 75 life threateningdiseases.

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Stem Cells Can Protect Us From

Blood disorders

Leukemia's Cancer.

Thalassemia

Heart stroke Diabetes

Spinal cord Injury

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Your baby's cord blood could save the lifeof the child as well as family member orsomeone else in need.

You only get one chance!

Thousands of adults and children are in

serious need of a life saving transplant. The possibility of medical advancement

relies on cord blood stem cells.

BANK OF STEM CELL

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A Joint Venture between CryobanksInternational Inc & RJ Group, India

R J Group is 15000 crore Group

RJ Corp

BeveragesEducation

Health

CareReal EstateHospitality

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North & South America

43 Systems

23 Cord Blood Banks5 Countries

Europe/Russia/Mid East

33 Systems


Asia

47 Systems


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First Cord Blood Bank which is licensed by Drug

ControllerGeneral of India& ISO Certified

28.000 sq. ft. area with 14,000 dedicatedlaboratory space

Provides complete in house processing and

storage technology, licensed by Cryobanks Intl.Inc. USA which is accredited by AABB, US FDA,

FACT NETCORD and NMDP

CRYOBANKS INTERNATIONAL INDIA

The Caitlin Raymond

International Registry

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Closed system of processing which preventschances of contamination of cord blood &High Viability counts in each cord blood unit

processed. Lab is completely class 10,000 sterile

laboratory premises.

National coverage We collect your unitfrom all major cities in India

Our highly qualified team of medicalprofessionals has been extensively trainedand certified in cord blood technology

Our lab operates 24X7We have several

flexible payment plans to help you protectyour family

CRYOBANKS INTERNATIONAL INDIA

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Stem Cells insured with Bajaj Allianze Generalinsurance co.

HLA matching facility available inG

ujarat Import export licensed for transferring the sample out

of India and vice a versa.

Dr. Sunil Advani (Hematologist, Mumbai) will beheading the transplant department at MedicityGurgoan from January 2009.

Doctor is available at all center ofGujarat

Transplant facility and transplant doctor is available inAhmedabad

Advantages of Cryobanks

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ADDRESS

129, PACECITY 1,SECTOR 37

GURGAON INDIATEL. 0124-4265030FAX: 0124-4370257TOLL FREE NUMBER

1-800-102-2796E-MAIL

[email protected]

CRYOBANKS INTERNATIONAL INDIAGURGAON CENTER

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In the words of Dr Trehan, Umbilical Cord

Bloodcontains millions of valuable stem cells that

can be used in many different ways to correctleukemia,

anemia, metabolic disorders, and many white

blood cell diseases. In the very near future,these cells could be used to regenerate heart

muscles and impact neurological diseasetreatments,

It is the future of medicine.

Dr.Naresh Trehan(M.D.)CARDIOLOGISTSTEMCELL SPECIALISTFOR CARDIACSURGERY

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Cryobanks International India Pvt. Ltd.Staff Members (Trained at U.S.A)

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Our kit is UN certified which means it can travelanywhere in the world

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Collection procedure

Pre Collection Set

up

The Placenta is

examined for gross

abnormalities

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ContinueContinue

The Cord is cleanedwith iodine at the

venipuncture site

The Cord is cleanedwith an absorbent

gauze followed byalchohol

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Blood drains bygravity into acollection bag

The umbilical veinis punctured with

the bevel of theneedle toward

the vein

ContinueContinue

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ContinueContinue

Tubing is clampedand needle cut off

Complete blood baglabel and affix

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ContinueContinue

Place labelled bloodbag and maternal

blood into plastic kitboxes

The box is then sealedand stored at room

temperature

DO NOT REFRIGERATE

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Incubator

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Cord Blood Processing

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Separator Unit for

Stem Cell

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Separation of Stem

Cell in close system

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Test Performed onMaternal Blood

HIV (I & II)

HBsAg

Anti HCV

Malaria

Syphilis

Anti HTLV (1&2)

Anti CMV IgM & IgG Maternal BloodGrouping

Tests Performed onCord Blood

TNCC-Total Nucleated Cell

CountViabilityCD34+ CountCord Blood groupingCord Blood culture for

microbial contamination

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Coulter for in-house

diagnostic testing of

the cord blood unit &

mother

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Flow cytometer for

cd34+ counts of stem

cell unit

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In house facility of

all diagnostictesting of unit

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Decreasing

refrigerator

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Vapour Phase

LN2 Storage Tanks

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Storage system ofstem cell in ln2 tank

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How Long Stem Cell can be stored

Stem Cell can be stored for 21 years asper the initial contract with the company.

Storage can be renewed after 21 years

Stem cell can be stored for indefinitetime.

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Likelihood of HLA match

Identical twin with same genetic complement- no match required

Sibling of same parents 1 in 4 chance of100% match

3/6 or 4/6 match sufficient for transplant

ANYONE ELSE IN THE FAMILY- no predictions

possible - similar to unrelated donors

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FIRST CORD BLOOD STEM CELL TRANSPLANTIN THE WORLD- A REALITY !

1988 in FranceElianeGluckman

first successful related UCB transplant, ofsibling in a 6 year old boy to cure Fanconis

Anemia

The boy is now a healthy teenThe boy is now a healthy teen

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HEMANT BEBERA-The First Cord Blood Stem Cell

Transplant in India

Has two birthdays.Has conquered thalassemia,Was trested on August 7, 2002

Army Hospital (Research andReferral), New Delhi

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MetachromaticLeukodystrophyMLD

Source of stem cells: matchedcord blood >3x10E6 CD34+cells. Need to minimizeGvHD.

Complications:

1. Severe gutGvHD, >2 months ofvery extensive diarrhea, extensivenutritional support for months.

2. Now almost 2 years from time oftransplant, perfectly normal, no

signs of any GvHD or MLD.

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Metachromatic LeukodystrophyMLD

1st child in family with juvenile MLD

Presented with signs/symptoms at age 4-5;died age 12.

2 subsequent pregnancies screened; werepositive & terminated

4th pregnancy said to be negative.Subsequent testing after birth showed that initialtesting was false negative.

Decision made to proceed with transplantprior to the onset of any signs and symptoms.

Genetic diseases generally run true in families,meaning that we should anticipate onset of

symptoms at about the same time as the sibling

who died.

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Myelodysplastic Syndrome

Transplanted in 2nd remission

1. Source of stem cells: No matched sibling

available so cord blood used at thecenter at which he was transplanted.

Use of cord blood helped expeditetransplant.

2. Delayed engraftment 3-4 weeks.

3. Initial Transplant hospitalization: fungalinfection

4. left hospital within 1 week with norecurrence of illness

Transpl. 7:395-399, 2005

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Blackfan-Diamond Syndrome or congenital absence of

erythropoiesis.

1. Chronically steroid dependent

2. Short stature, pseudotumor cerebri, osteoporosis,multiple fractures, AVN, infection risks, iron overload

neg.

3. Stem cell source: cord blood to minimizeGvHD.

4. Mother pregnant: stem cells collected at birth of

sibling5. Stem cells used: cord blood 1x10E6 CD34+cells/kg

plus marrow 2x10E8 nucleated cells/kg; 100%chimerism

6. GvHD: skin, resolved. Steroids gone after 6 months.

7. Outcome: fractures healing, on growth hormone,

attending school, playing soccer, no infections.

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Genetic ImmunodeficiencyDisease*

Patients with SCID, CID,CVID, Wiscott AdrichSyndrome, Leukocyte

Adhesion deficiency, etc.

recover full immunefunction (both T and Bcells) after unrelatedcord bloodtransplantation.

Shown at 3 years

post transplant

Transplanted for WASAt age 18 months

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Metabolic Disease MPS-1 (HurlerSyndrome)*

CB transplant arrestsprogression andreverses damage inthe cornea, liver,bones, cartilage,

hearts and brains ofyoung patients withHurler Syndromewhich otherwisecauses death by 5-6years of age.

Shown at 6 yearspost transplant

IQ147

Transplanted with UCB

at 5 months of age

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Genetic DiseaseSickle Cell Anemia & Thalassemia

4 years Post Transplant

for Thalassemia

Unrelated CB Tx at2.5 months Both related and

unrelated cord bloodtransplants cansuccessfully treatchildren withhemoglobinopathies,

correcting thedefect. If thetransplantIs performed early inlife, survivals are>80%.

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MetabolicDisease/Krabbe

Disease

(GloboidLeukodystrophy)

This child is now neurologicallynormal at age 5. Two year oldaffected sibling died at age 2.

Transplanted at 3 weeks of age

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The Blood Brothers-

Nicholas andNathaniel Henderson

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CCMB, LVPEI and Sarojini Eye Hospital

Wednesday, March 02, 2005CCMB eyes on creating cornea in-vitro

250 patients who had undergonestem cell therapy - 70 % had visionrestored.

The next step Develop a hemi-cornea

Final goal- Reconstruct an entirecornea from a suitable stem cell

source

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TIMES NEWS NETWORK- SATURDAY, FEBRUARY 26, 2005

1. Cardiomyopathy

2. Sroke patient3. Insulin-dependent diabetes

have been given stem cell therapy at

AIIMS

As of now , 360 patients have

undergone this Therapy,says Director of AIIMS

.. Dr. Venugopal

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Transendocardial, autologous Stem Cell transplantation of

severe, chronic IHD- Circulation 2003, 107 :2294 - 2302

35 cardiac patients who only had

chances Of surviving after a hearttransplant instead Stem Cell therapywere given and all patients survived.

64% of their dead heart muscles wererevived in 18 months

Severe Heart Failure Treatment

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Applications in Plastic Surgery

Fillers Breast implants

Reconstruction of bone, cartilage

Skin graft- in burns and other skin

diseases Baldness

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Growth in UmbilicalCord Blood

Stem Cell Therapeutic

Use

Growth in UmbilicalCord Blood

Stem Cell Therapeutic

Use

0

1000

2000

3000

4000

5000

6000

7000

8000

1994 1995 1996 1997 1999 2001 2003 2005 2006

Annual

Cord Blood Stem CellTransplants

(Worldwide)

Perlow JH. Stem Cell Reviews; November 2006

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Stem Cell therapy centres in India

o Gujarat Cancer & Research Institute, Gujarato Tata Memorial Hospital, Mumbaio Adyar Cancer Centre, Madraso Apollo Specialty Hospital, Madras,o Apollo Hospital, Global Hospitals, NIMS, Hyderabado Christian Medical College, Vellore

o Narayana Hruduyalaya , Bangaloreo R&R Army Hospital, New Delhio AIIMS , New Delhio Inlaks Hospital, Puneo Armed Forces Medical College, Puneo Sanjay Gandhi PGIMS, Lucknow

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Diseases Treatable by

Stem Cell Transplant

Hematologic malignancies

Leukemia, lymphoma

Immunodeficiency diseases SCID, CID, CVID, WAS

Bone marrow failure syndromes

Fanconi Anemia, Severe aplastic anemia

Hemoglobinopathies Sickle Cell Anemia

Thalassemia Major

Inborn errors of metabolism

ADL, MLD, GLD MPS I, II, III, VI

Tay Sachs Disease

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Diseases Treatable by StemCell

Transplant

Refractory Anemia (RA)

Refractory Anemia with RingedSideroblasts (RARS)

Refractory Anemia with ExcessBlasts (RAEB)

Refractory Anemia with ExcessBlasts in Transformation (RAEB-

T)

Chronic MyelomonocyticLeukemia (CMML)

Aplastic Anemia (Severe)

Fanconi Anemia

Paroxysmal NocturnalHemoglobinuria (PNH)

Acute Lymphoblastic Leukemia(ALL)

Acute Myelogenous Leukemia

(AML) Acute Biphenotypic Leukemia Acute Undifferentiated

Leukemia Chronic Myelogenous

Leukemia (CML) Advanced Chronic

Lymphocytic Leukemia (CLL) Juvenile Chronic Myelogenous

Leukemia (JCML)

Juvenile MyelomonocyticLeukemia (JMML)

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Stem Cell

Transplant


Stem Cell

Transplant

Familial Erythrophagocytic

Lymphohistiocytosis Histiocytosis-X

Hemophagocytosis Beta Thalassemia Major

Pure Red Cell Aplasia Ataxia-Telangiectasia

Kostmann Syndrome Leukocyte Adhesion Deficiency

DiGeorge Syndrome Bare Lymphocyte Syndrome

Omenn's Syndrome Sickle Cell Disease

Severe CombinedImmunodeficiency (SCID)

SCID with AdenosineDeaminase Deficiency

Absence of T & B Cells SCID

Absence of T Cells, Normal BCell SCID

Common VariableImmunodeficiency

Wiskott-Aldrich Syndrome

X-Linked Lymphoproliferative

Disorder Lesch-Nyhan Syndrome

Cartilage-Hair Hypoplasia

Glanzmann Thrombasthenia

Osteopetrosis

Amegakaryocytosis /

Congenital Thrombocytopenia

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Emerging therapies

Heart Disease

Diabetes Muscular Dystrophy

Multiple Sclerosis

Alzheimers

Parkinsonism Spinal Cord Injuries

Lupus

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The Registration Process:-Paper Work andPayment

Pre-Collection:-Cord Blood Collection and TransportKit

On The Way to the Hospital:-Make a Phone Callto Cryobanks Relationship Executive

Collection Process:-The doctor in charge performs

the collection process Shipping of Cord Blood:- Responsibility of

Cryobanks Relationship Executive

Processing and Storage

Steps To Follow :-

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Documents

135136_633671881158745000