11_Magneticky Modifikovane Aktivni Uhli a Biouhel_SAFARIK

7/13/2019 11_Magneticky Modifikovane Aktivni Uhli a Biouhel_SAFARIK

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Magneticky modifikovan

aktivn uhl a biouhel a jejichvyuit

Ivo afak, Kateina Horsk,Kristna Pospkov, Zdenka Madrov,

Mirka afakov

Oddlen nanobiotechnologiestav nanobiologie a strukturn biologie CVGZ AVR

esk Budjovice

[email protected]

www.nh.cas.cz/people/safarik


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Types of magnetic nano- and

microparticles

Multi domain, single domain or

superparamagnetic

Magnetite (Fe3O4)

Ferrites (MeO . Fe2O3;Me = Ni, Co, Mg, Zn, Mn ...)

Maghemite (-Fe2O3)

Greigite (Fe3S4)

Iron, nickel


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Why magnetic materials are so

important in bioapplications?

They are smart materials!!!!

The following typical properties ofmagnetic materials form the basis

of their applications in biosciencesand biotechnology


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Important properties

Selective separation(removal) of magneticparticles from thesystem

Targeting (navigation)of magnetic particles

to desired area usingmagnetic field


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keeping magneticparticles in

appropriate area

using magnetic field

Heat formation inalternated magnetic

field
http://images.google.com/imgres?imgurl=http://www.biomagres.com/content/figures/1477-044X-1-2-3.jpg&imgrefurl=http://www.biomagres.com/content/1/1/2/figure/F3&h=256&w=300&sz=30&hl=cs&start=1&um=1&tbnid=K7ADwlcaRzc7XM:&tbnh=115&tbnw=135&prev=/images%3Fq%3Dmagnetic%2Bdrug%2Btargeting%26svnum%3D10%26um%3D1%26hl%3Dcs%26newwindow%3D1%26sa%3DX


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Increasing of contrastduring MRI

Peroxidase-likeactivity


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Hardening ofbiological structures

(chiton teeth)

Navigation inmagnetic field


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Magnetic labeling ofbiologically activecompounds

Magnetization ofbiologicaldiamagnetic materials


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Examples of of magnetic nano- and

microparticlesapplications

From molecular biology to environmentaltechnologies

Manipulation of microliters as well asmillion of liters

Manipulation in suspension systems

Both separation and non-separationtechniques are important


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Preparation of magnetic particles

for bioapplications Precipitation High-temperature reactions Reactions in steric environments Sol-gel reactions

Decomposition of organometallic precursors Polyol methods Biosynthesis

Laurent S, Forge D, Port M, Roch A, Robic C, Elst LV, Muller RN: Magnetic ironoxide nanoparticles: Synthesis, stabilization, vectorization, physicochemicalcharacterizations, and biological applications. Chem Rev 2008, 108(6):2064-2110.


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Postmagnetization

Chemical precipitation procedures

High temperature treatment

Ferrofluid treatment Microwave assisted procedures

Mechanochemistry

Encapsulation


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Review paper


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Conversion Of Activated Carbons (Charcoal) Into Their Magnetic

Derivatives Using Chemical Precipitation Procedures

Modification Procedure

Precipitation of magnetite from FeSO4 and Fe2(SO4)3 by NaOH in the presence of charcoal, followed by

aging for 24 h and heating at 473 K

Precipitation of iron oxides from FeSO4 and FeCl3 by NaOH in the presence of charcoal, followed by

drying at 100 C for 3 h

Precipitation of hydrated iron oxides from FeSO4

by NaOH in the presence of charcoal, followed by

heating to 100 C for 1 h

Activated carbon was suspended in NaOH solution and heated to 100 C; then a solution of Fe(NO3)3

and Co(NO3)2 was quickly poured into the AC suspension and refluxed at 100 C for 2 h

Bamboo charcoal powder was suspended in Fe(NO3)3, Zn(NO3)2, Ni(NO3)2 and aqueous ammonia

solution and then heated in an autoclave at 180 C for 2 h and air cooled to room temperature

Activated carbon was suspended in CuCl2and FeCl3solution, followed by NaOH solution addition and

heating to 98-100 C for 2 h

FeCl3 and FeSO4solution was mixed with NaOH solution to keep pH value of 9.5, then activated carbon

was added and the obtained material was dried in an oven at 100 C for 3 h


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Activated carbon was impregnated with an aqueous solution of sucrose and Ni(NO3)2, followed by heating at 600 Cunder N2for 3 hours. Ni nanoparticles were formed within the porous AC matrix

A solution of Ni(NO3)2was dropped into NaOH solution, then ethanol solution of phenolic resin was added followed by

solvent evaporation at 333 K and carbonization under argon atmosphere at 873 K

Impregnation of activated carbon with Fe(NO3)3 solution followed by drying at 90 C and heated to 700 C under

argon; then benzene vapor was introduced

Activated carbon from rice husk was modified with HNO3 for 3 h at 80 C followed by suspending in Fe(NO3)3anddrying. Thermal treatment was conducted at 750 C for 3 h in the presence of N2to enable formation of magnetite

nanoparticles

Dried chitosan microspheres were immersed in (NH4)3[Fe(C2O4)3] solution followed by washing and drying, then the

sample was carbonized under Ar atmosphere at 700-1000 C for 4 h

Activated carbon was suspended in Fe(NO3)3; after drying it was heated to 800 C in N2 atmosphere and after cooling

heated at 850 C in CO2 atmosphere for 1.5 h

A mixture of the anthracite powder, coal tar, Ni(NO3)2 and water was mixed and extruded in the form of 1 cm

cylinders. After drying the material was carbonized under a flow of N2 at 600 C and then activated at 880 C

under a flow of N2

Activated carbon was impregnated with Fe(NO3)3 solution and then with ethylene glycol. The impregnated sample was

subjected to heat treatment under N2atmosphere at a temperature 250-450 C for 2 h

Activated carbon was filled with a Fe(NO3)3 solution in ethanol and then dried at 90 C for 2 h. Then the sample was

impregnated with ethylene glycol followed by heat treatment under N2 atmosphere at a temperature 350 or 450C for 2 h

Conversion Of Activated Carbons (Charcoal) Into Their Magnetic Derivatives By

High Temperature Treatment


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Conversion Of Activated Carbons (Charcoal) Into Their

Magnetic Derivatives By Encapsulation


Activated carbon was mixed with alginate solution and citrate stabilized ferrofluid and then the suspension was

added dropwise into a CaCl2 solution

Cellulose was dissolved in a cooled NaOH/urea solution followed by the addition of maghemite nanoparticles

and activated carbon; the suspension was added dropwise into a NaCl solution. The formed beads were cross-

linked with epichlorohydrin

Charcoal and magnetisable ferric oxide were entrapped in a polyacrylamide gel followed by lyophilisation and

micronisation

Charcoal and barium ferrite microparticles were mixed with bovine serum albumin solution followed by

emulsification in n-butanol castor oil glutaraldehyde continuous phase

Charcoal and magnetisable ferric oxide were entrapped in a polyacrylamide gel followed by drying at 80 C

overnight and milling to obtain particles of less then 50 m in diameter

Activated carbon was suspended in NaOH solution and heated to 100 C; then a solution of Fe(NO3)3and

Co(NO3)2 was quickly poured into the AC suspension and refluxed at 100 C for 2 h. This material was added

to Na alginate solution followed by pouring dropwise into CaCl2 solution


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Application Of Magnetic Activated Carbons (Charcoal) For The Separation Of

Organic CompoundsType of MAC Separated organic compound

Almond shells 2,4,6-Trinitrophenol from water; 97% desorption achieved by methanol and hot water

Orange peel Naphthalene and p-nitrotoluene

Commercial Methylene blue from river water; maximum adsorption capacity was 47.62 mg g-1

Hydro-thermal process Methyl orange from water; maximum adsorption capacity was 44.65 mg g-1

Coconut shell Humic substances

Bitumine Methylene blue; maximum adsorption capacity was 229.5 mg g-1

Commercial Adsorption of methylene blue by activated carbon/cobalt ferrite/alginate composite beads

Chezacarb B Water soluble organic dyes from aqueous solutions

Chezacarb B Crystal violet and safranin O; magnetic solid-phase extraction used for preconcentration

Palm shells Oil from palm oil mill effluent

Commercial (Norit) Imidacloprid from water

Phenolic resin Methylene orange from water; maximum adsorption capacity was 0.16 mg m-2

Coconut shell Methyl orange from water; regeneration by hydrogen peroxide performed

Rice husk Methylene blue from water, maximum adsorption capacity was 321 mg g-1

Commercial Malachite green from water; maximum adsorption capacity was 89.29 mg g-1


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Application Of Magnetic Activated Carbons (Charcoal) For

The Separation Of Inorganic Compounds

Type of MAC Separated inorganic compound

Coconut shell

Mercury; maximum adsorption capacity was 38.3 mg g-1. Hg desorption can be

performed by heating

Bituminous coal Mercury(II) from water

CommercialArsenic(V) removal from contaminated water with MAC coated with bacteria or

biopolymers

Coconut or fruit pit Gold from cyanide leach liquor or cyanide pulp

Orange peel Phosphate from wastewater


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Microwave assisted synthesis of

magnetically responsive biochar

Biochar

Ferrous sulfate

Microwave oven

high pH

Magnetic properties are caused by the deposition of magnetic iron oxides nano-

and microparticles on the biochar surface using the developed procedure

Magnetic biochar

Fe2++ H2O Fe(OH)2

3 Fe(OH)2+ O2 Fe3O4+ 3 H2Omicrowave


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Safarik,I., Horska,K., Pospiskova,K., Maderova,Z., Safarikova,M.:

Microwave Assisted Synthesis of Magnetically Responsive Composite

Materials. IEEE Trans. Magn. 49 (1) (2013) 213-218


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Magnetic derivative of biochar


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Adsorption of acridine orange

Akridinov oran 50mg mag.biochar

0

5

10

15

20

25

30

0 10 20 30 40 50 60

Ceq ( mg/l)

Qeq(mg/g)


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Sirofloc


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COST Action (do 25. 3. 2016)


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MC


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!!!!!

[email protected]

www.nh.cas.cz/people/safarik

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11_Magneticky Modifikovane Aktivni Uhli a Biouhel_SAFARIK