10th International Luebeck Conference on the ... · PDF file10:20 – 10:40 Henrik Martens, Tekla Kolbow, Christo ph Ott, Hannelore Ehrenreich ... “Epicardial Erythropoietin release

UNIVERSITÄT ZU LÜBECK

INSTITUT FÜR PHYSIOLOGIE

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10th International Luebeck Conference on the

Pathophysiology and Pharmacology of Erythropoietin

and other Hemopoietic Growth Factors

Music & Congress Centre of Luebeck, Germany

June 3 - 5, 2015

Under the auspices of the German Physiological Soci ety (DPG)



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General Information The 10th International Luebeck Conference on the Pathophysiology and Pharmacology of Erythropoietin and other Hemopoietic Growth Factors will be held at the Music & Congress Centre of Luebeck (Musik- und Kongresshalle - MUK), Germany, from June 3 - 5, 2015. Similar to the meetings before, the purpose of the conference will be to stimulate the exchange of ideas between basic scientists and clinicians who are active in this field. The major scientific topics will be: - Erythropoietin receptor biology - Hypoxia sensing - Erythropoietin production - Novel erythropoiesis-stimulating agents - Use in the treatment of anemias - Sports medicine - Colony-stimulating factors Date and Place June 3 - 5, 2015 of the Meeting Music & Congress Centre Willy-Brandt-Allee 10 23554 Luebeck Germany Organization Horst Pagel and Wolfgang Jelkmann

Institute of Physiology University of Luebeck Ratzeburger Allee 160 D-23562 Lübeck Germany

Permanent Office Evelyn Kimpel, Secretary

Phone: ++49-451-500-4152 Fax: ++49-451-500-4151

E-mail: [email protected]

Presentations Oral presentations: in English only (no translation), 15 min plus

5 min for discussion (unless otherwise noted). Please deliver your presentation on a stick on Thursday morning prior to the start of the sessions.



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Poster presentations: The posters will be up on Thursday and Friday (mounting on Thursday morning) in the foyer of the Music & Congress Centre. Poster authors should be present during the Poster Session on Thursday afternoon and the Poster Question and Answer Session on Friday morning. There will be no guided poster tour. However, participants may hand over questions in written form to the secretarial congress office during the meeting for discussion on Friday morning (Q & A).

A poster award committee will evaluate the posters for a poster prize. The winner will be announced during the Q & A session on Friday morning.

For the presentations of the posters a board will be available (120 cm width, 150 cm height). Fixing material will be provided by the organizers.

Lunch and Coffee Coffee, tea, refreshments, sandwiches and snacks will be

offered free of charge.



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How to get to Lübeck By airplane:

Luebeck airport: The Hungarian-Polish airline Wizz Air flies from/to Luebeck from or to Danzig (Poland) and Kiev (the Ukraine). Further information at www.wizzair.com.

Getting from Luebeck airport to Luebeck's town centre is easy: The number 6 bus route starts right in front of the Luebeck airport and leaves every 20 min for the central bus station and back (journey approx. 30 min). A train leaves for the main train station every hour from the "Lübeck Flughafen" stop and back again (journey approx. 8 min).

Hamburg Airport is the closest major international airport.

From Hamburg Airport you can get the S-Bahn (suburban rail network) line S1. Line S1 operates every 10 min between Hamburg Airport and Hamburg’s central railway station, Hauptbahnhof, a journey of 25 min. Trains from Hamburg central station to Luebeck central station operate from 8 am - 8 pm, every 30 min and before and after that every hour (journey approx. 40 min). The “Hamburg Airport (Flughafen)” S-Bahn station is directly in front of the terminals and is easily reached by lift, escalator or stairs.

By train: Trains from Hamburg arrive at Luebeck main station every hour. From there you can reach the meeting location within a 5-10 min walk.

By car: You can reach Luebeck via Autobahn A1 from Copenhagen and Hamburg (exits „Lübeck Zentrum“ or „Lübeck Moisling“) or via A20 from Rostock, A24 from Berlin and change in Hamburg onto the A1.

The Stadtverkehr Lübeck GmbH operates the public transport system in Luebeck and the surrounding area. Information on timetables, tickets and tariffs are available from www.stadtverkehr-luebeck.de, www.scout-sh.de or Tel.: +49 (0) 4 51/8 88 28 28. Accommodation For hotel arrangements, please contact the tourist board:

http://www.luebeck-tourism.de/



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Wednesday, June 3, 2015 19:00 Reception evening with catering

Audience Hall ("Audienzsaal") of the Town Hall ("Rathaus") of the Hanseatic City of Luebeck, Breite Straße 62 Welcome by Mr. Bernd Saxe, Mayor of the City of Luebeck



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Scientific Program Thursday, June 4, 2015 8:30 Opening Addresses Wolfgang Jelkmann Mathias Freund, President of the German Society of Hematology and Oncology (DGHO) 9:00 – 10:40 Session 1 ( Oral Presentations) "Detection of erythropoietin receptor" (Chairpersons: Constance Noguchi, Michael Jarsch ) 9:00 – 9:20 Nina Trošt, Neli Hevir, Patrícia Kimáková, Lenka Hurtuková, Barbora Fecková, Tea Lanišnik Rižner, Radovan Komel, Peter Solár, Nataša Debeljak “Erythropoietin receptor modulates breast cancer cell response to tamoxifen treatment” 9:20 – 9:40 Andre Bernardini, Ulf Brockmeier, Erik Metzen, Joachim Fandrey “Study of erythropoietin receptor activation in living cells by Förster resonance energy transfer microscopy” 9:40 – 10:00 Perry Maxwell, Robert J Cuthbert, Terence R Lappin “Sensitive and specific probes for the erythropoietin receptor in clinical samples” 10:00 – 10:20 John Thompson, Fritz Grunert “Development and specificity testing of novel antibodies directed

against the human erythropoietin receptor” 10:20 – 10:40 Henrik Martens, Tekla Kolbow, Christoph Ott, Hannelore Ehrenreich “New polyclonal and monoclonal antibodies against the human and murine EPO-receptor” 10:40 – 11:10 Coffee and Tea Break



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11:10 – 12:40 Session 2 ( Oral Presentations) "Pleiotropic actions of erythropoietin" (Chairpersons: Reinhard Depping, Joachim Fandrey ) 11:10 – 11:30 Christian Klopsch, Marion Ludwig, Heiko Lemke, Gabriela Kleiner, Anna Skorska, Ralf Gaebel, Christian Maschmeier, Sarah Müller, Stefan Jockenhoevel, Brigitte Vollmar, Robert Jaster, Robert David, Gustav Steinhoff “Epicardial Erythropoietin release improves cardiac functions and enhances rapid proliferation and tissue transformation in the intracardiac mesenchyme after myocardial infarction” 11:30 – 11:50 Mete Özkurt, K Uzuner, N Erkasap, W Jelkmann, R Ozyurt, G Kus, O Uysal, O Kutlay “Investigation of erythropoietin, TNF-α and kidney tissue injury connections in L-NAME induced hypertensive rats” 11:50 – 12:10 Constance Tom Noguchi, Yuanyuan Zhang, Heather Rogers, Li Wang, Mawadda Alnaeeli “Erythropoietin and metabolic response” 12:10 – 12:30 Martina Rauner, Kristin Franke, Rashim Pal Singh, Sahar Hiram-Bab, Max Gassmann, Drorit Neumann, Yankel Gabet, Triantafyllos Chavakis, Lorenz C. Hofbauer, Ben Wielockx “Bone homeostasis is regulated by the PHD2 - HIF2α - EPO axis” 12:30 – 12:50 Sahar Hiram-Bab, Tamar Liron, Naamit Deshet-Unger, Moshe Mittelman, Max Gassmann, Martina Rauner, Ben Wielockx, Drorit Neumann, Yankel Gabet “EPO attenuation of bone formation: contribution of non-osteoblastic cells?”

12:50 – 14:00 LUNCH

14.00 - 15.40 Session 3 ( Oral Presentations) "Effects of erythropoietin on neuronal cells" (Chairpersons: Anna-Leena Sirén, Henrik Oster) 14:00 – 14:20 Nina Hahn, Hannelore Ehrenreich, Ralf Heinrich “Erythropoietin in insects: receptors, signaling pathways and neuroprotection” 14:20 – 14:40 Christoph Ott, Imam Hassouna, Liane Dahm, Sandra Goebbels,



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Henrik Martens, Klaus-Armin Nave, Hannelore Ehrenreich “Inducible formation of hippocampal oligodendrocytes from preexisting local precursors by erythropoietin” 14:40 – 15:00 Imam Hassouna, Christoph Ott, Liane Dahm, Nils Offen, Daniela Winkler, Ekrem Dere, Henrik Martens, Klaus-Armin Nave, Anna-Leena Sirén, Hannelore Ehrenreich “Erythropoietin induces formation of hippocampal CA neurons from preexisting local precursors” 15:00 – 15:20 Jean-Claude Fauchère, Russia H. Leuchter, Giancarlo Natalucci, Christof Dame, Brigitte M. Koller, Christoph M. Rüegger, Cornelia Hagmann, Petra S. Hüppi, Hans Ulrich Bucher on behalf of the ‘The Swiss EPO Neuroprotection Trial Group'. “Results of the Swiss Epo Neuroprotection Trial in Very Preterm Infants” 15:20 – 15:40 Kamilla W. Miskowiak “Neural correlates of improved executive function following erythropoietin treatment in mood disorders” 15:40 – 16:10 Coffee and Tea Break 16:10 – 17:50 Session 4 ( Oral Presentations) "Cellular responses to hypoxia" (Chairpersons: Dörthe Katschinski, Bernhard Brün e) 16:10 – 16:30 Markus Mandl, Maria Lieberum, Reinhard Depping ”Cell-specific upregulation of the transcription factor ARNT (HIF-1β) in response to hypoxia” 16:30 – 16:50 Alexandra Schörg, Sara Santambrogio James Platt, Johannes Schödel, Maja T. Lindenmeyer, Clemens D. Cohen, Katrin Schrödter, David R. Mole, Roland H. Wenger, David Hoogewijs “Destruction of a distal hypoxia response element abolishes trans- activation of the PAG1 gene mediated by HIF-independent chromatin looping” 16:50 – 17:10 Nathalie Dehne, Bernhard Brüne „Hypoxic inhibition of JMJD3 reduces H3K27me3 demethylation, chromatin remodeling, and induction of the STAT6 target gene Ccl18” 17:10 – 17:30 Dominik C. Fuhrmann, Nathalie Dehne, Bernhard Brüne “Regulation of protein expression under chronic hypoxia”



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17:30 – 17:50 Emma J Hodson, Lynn G Nicholls, Philip J Turner, Peter A Robbins, Christopher W Pugh, Keith Buckler, Peter J Ratcliffe, Tammie Bishop “Contrasting roles of HIF-1 and 2 in ventilatory acclimatisation to hypoxia” 17:50 – 19:00 Session 5 (Poster Viewing): 21 K. Gottwald, V. Biedermann, S. Seeleithner, A. Gross, M. Rädisch, W. Jelkmann, C. Unverzagt "Semisynthesis of two glycoforms of human erythropoietin" 22 Maria- Katharina Lieberum, Markus Mandl, Jürgen Dunst, Reinhard Depping "The transcription factor ARNT influences radiation sensibility in different cell lines" 23 Jonas J. Staudacher, Isabel S. Naarmann-de Vries, Stefanie J. Ujvari, Bertram Klinger, Mumtaz Kasim, Edgar Benko, Antje Ostareck- Lederer, Dirk H. Ostareck, Pontus B. Persson, Ralf Mrowka, Michael Fähling "Transcriptome partitioning for mRNA translation in hypoxia" 24 Liane Dahm, Miso Mitkovski, Ralf Heinrich, Imam Hassouna, Christoph Ott, Henrik Martens, Klaus-Armin Nave, Hannelore Ehrenreich "Erythropoietin dampens injury-induced microglial motility" 25 Friederike Katharina Kosyna, Marie Nagel, Maria Lieberum, Larissa Kluxen, Kim Kraushaar, Reinhard Depping "The importin α/β-specific inhibitor Ivermectin affects HIF-dependent hypoxia response pathways" 26 Sina Schultz, Mahtab Ahmad Khan, Markus Schwaninger "The decrease of M-CSF expression in the brain endothelium by acute hyperglycemia leads to a reduction in tissue protective M2 macrophages after ischemic stroke" 27 Birgül Kurt, Katharina Gerl, Dominik Steppan, Lucile Miquerol, Armin Kurtz "Ectopic hyperplastic renin expressing cells can transform into erythropoietin expressing cells at any time" 28 Wolfgang Jelkmann, Gerhard Lonnemann "Relationship between plasma activin A levels and excretory renal function"



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29 Pamela J. Kling, Allison Pollock, Daphne D-Q Pham, Sharon E. Blohowiak "Cord blood erythropoietin levels after maternal obesity during gestation" 30 Demikhov V.G., Lunyakova M.A., Beznoshchenko A.G., Scobin V.B. "Effect of rHuEPO therapy on long-term survival of children with acute lymphoblastic leukemia, undergoing chemotherapy according to the program ALL BFM-90m" 31 Link H., A. Lück, J. Rauh, D. Bòrquez, C. Maas, J. Hesse, M. Koenigsmann, M. Schulze, M. Tölg "Multicentre, non-interventional study with epoetin theta in patients with chemotherapy-induced symptomatic anemia (EPO-amb) conducted in Germany" 32 Patrícia Kimáková, Lenka Hurtuková, Barbora Fecková, Lenka Varinská, Zuzana Solárová, Nataša Debeljak, Peter Solár "Angiogenic potential of hypoxic conditioned media from ovarian adenocarcinoma A2780 cells: the effect of erythropoietin" 33 Agnieszka Zembron-Lacny, Anna Kasperska, Barbara Morawin, Andrzej Pokrywka, Zbigniew Szygula "Effect of intermittent hypoxic training on erythropoietin level in relation to inflammatory response" 34 Elmo Neuberger, Martin Mooses, Jerome Durussel, Jun Wang, Mark Lees, Kerli Mooses, Alexandra Brahmer, Guan Wang, Yannis Pitsiladis, Perikles Simon "Small RNA sequencing of red blood cell specific miRNAs reveals expression changes after 6 weeks of recombinant EPO (rEPO) administration in healthy recreational athletes" 19:00 – ∞∞∞∞ DINNER Friday, June 5, 2015 8:30 – 9:00 Session 6 Q & A: Answers of the poster presenters to submi tted questions - open discussion. Poster award announcement. (Chairperson: Horst Pagel)



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9:00 – 11:00 Session 7 ( Oral Presentations) "Production of erythropoietin" (Chairpersons: Birgül Kurt, Thomas Hellwig-Bürge l) 9:00 – 9:20 Roland H. Wenger "Regulation of the EPO Gene" 9:20 – 9:40 Hanako Kobayashi, Thomas C. Binns, Qingdu Liu, Volker H. Haase "Distinct populations of FoxD1-derived peritubular interstitial cells regulate erythropoietin (EPO) production in the kidney" 9:40 – 10:00 Masayuki Yamamoto "Renal erythropoietin producing cells for anemia and fibrosis" 10:00 – 10:20 Katharina Gerl, Lucile Miquerol, Vladimir T. Todorov, Christian P.M. Hugo, Ralf H. Adams, Armin Kurtz, Birgül Kurt "Inducible glomerular erythropoietin production in the adult kidney" 10:20 – 10:40 Wenwen Sun, Friederike Katharina Kosyna, Wolfgang Jelkmann, Reinhard Depping "Prolyl-4-hydroxylase 2 potentially contributes to hepatocellular carcinoma-associated erythrocytosis by maintaining hepatocyte nuclear factor-4α expression" 10:40 – 11:00 Teresa Otto, B. Delos Reyes, S. Lauterbach, Joachim Fandrey "EPO and EMT - new insights in EPO regulation" 11:00 – 11:20 Coffee and Tea Break 11:20 – 12:40 Session 8 ( Oral Presentations): "Pharmacology and molecular biology" (Chairpersons: Pamela Kling, Vladimir Skobin) 11:20 – 11:40 Anatole Besarab, K.-H. Peony Yu, Thomas B. Neff, Lynda Szczech "Clinical Experience with Roxadustat, a HIF-PHI, in the treatment of CKD associated anemia" 11:40 – 12:00 Ken Aizawa, Yoshihito Tashiro, Ryohei Kawasaki, Kenji, Yogo, Michinori Hirata, Koichi Endo, Yasushi Simona "Epoetin beta pegol retains its biological activity in the presence of the sialic acid metabolic enzyme sialidase" 12:00 – 12:20 Christel Bonnas, Daniela Winkler, Christoph Ott, Katja Specht, Inge Sillaber, Hannelore Ehrenreich, Herbert Stadler "A non-hematopoietic erythropoietin variant expressed in human



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blood" 12:20 – 12:40 Bojana Beleslin Cokic, Constance Tom Noguchi, Vladan Cokic, Svetozar Damjanovic "The effect of inactivated VHL gene on proliferative pathways in renal cell carcinoma" 12:40 – 13:20 LUNCH 13:20 – 13:50 Special Lecture on Sports Physiology (Chairperson: Wolfgang Jelkmann) Zbigniew Szygula: “Erythrocytic system under the influence of sports training - sports anemia, hypoxic training and blood doping” 13:50 – 14:00 Break 14:00 – 15:30 Session 9 ( Oral Presentations) "Recent clinical knowledge"

(Chairpersons: Valeriy Demikhov, Terence Lappin ) 14:00 – 14:20 Katarzyna A. Lisowska, Aleksandra Jasiulewicz, Alicja Dębska-Ślizień, Jacek M. Witkowski "Phenotype and proliferation capacity of CD4+ T and B cells of patients with end-stage renal disease depending on treatment with recombinant human erythropoietin" 14:20 – 14:40 Howard S. Oster, Naamit Deshet-Unger, Sara Prutchi-Sagiv, Nir Maaravi, Nataliya Golishevski, Drorit Neumann, Moshe Mittelman "Erythropoietin treatment is associated with improved immune parameters in patients with lower risk myelodsyplastic syndromes" 14:40 – 15:00 Naamit Deshet-Unger, Sahar Hiram-Bab, Yasmin Haim Ohana, Moshe Mittelman, Yankel Gabet, Drorit Neumann "Erythropoietin in 5T33 murine multiple myeloma: Opposing Immune and skeletal outcomes" 15:00 – 15:30 Howard S. Oster, Drorit Neumann, Moshe Mittelman "Erythropoietin: a burden or an asset?" 15:30 Concluding remarks



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Social Program The events are free of charge for participants of the conference.

Wednesday, June 3, 2015:

19.00 - 21:30 Get-together Party Audience Hall ("Audienzsaal") of the Town Hall ("Rathaus") of the Hanseatic City of Luebeck, Breite Straße 62 Welcome by Mr. Bernd Saxe, Mayor of the City of Lüb eck The Town Hall of Luebeck was commenced by the citizens in 1230 and was modified and extended during the following centuries. Hence, it incorporates a variety of styles from the Gothic to the Renaissance. Today the Town Hall still meets its purpose as domicile of the municipal parliament, the Senate, and the top-departments of the administration. In former times the Audience Hall used to be the convention room of the Hanseatic High Court. Its present outfit and equipment of the Rococo style originates from the years between 1754 and 1761. The paintings by the Italian artist Stefano Torelli show allegorical motives: the liberal arts, trade, freedom, mercy, harmony, industry and abundance, vigilance, intelligence, moderation, and discretion. The interior portal of the Audience Hall dates to 1573 and shows in reliefs the judgement of Solomon, the justice and affection. The portal had/has two openings: Those convicted had to stoop to pass through the lower door, but those acquitted could walk upright through the high one.

Thursday, June 4, 2015:

19.00 - ∞∞∞∞: Dinner Congress & Music Centre

Friday, June 5, 2015:

17:00 – 19:00: Sightseeing walk We invite you to join us for a leisurely stroll of about 2 hours through medieval Hanseatic Luebeck. We will show you its most beautiful corners, such as typical brick-buildings, courtyards and city-gates. UNESCO declared the existing cityscape to be a World Cultural Heritage Site in 1987.



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Saturday, June 6, 2015:

10:00 – 12:00: European Hansemuseum Lübeck Location: An der Untertrave 1 In this new museum (opened by end of May 2015) we will go on a unique trip through 600 years of Hanseatic history. Extending across northern Europe and the Baltic, the Hanse was a powerful economic alliance, in which first merchants and later towns joined together to promote trade. Modern museum technology, original exhibits, media stations, reconstructed scenes as well as graphics and statistics will explain the historical connections (www.hansemuseum.eu.).

Documents

10th International Luebeck Conference on the ... · PDF file10:20 – 10:40 Henrik Martens, Tekla Kolbow, Christo ph Ott, Hannelore Ehrenreich ... “Epicardial Erythropoietin release