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MLAB 2401: Clinical Chemistry
Chapter 1: Laboratory Safety and Regulations
Introduction
The chemical reagents that are used can be dangerous if not used properly Caustic / corrosive
Patient specimens must be handled with caution due to the possibility of infectious agents HIV, Hep B, meningitis, antibiotic resistant
pathogens, etc
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Minimize Risks by:
Everything must be labeled and stored appropriately
It’s essential to know the location and proper use of all safety equipment
It’s essential to know and have access to all printed information about safety policies and reagents
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LABORATORY SAFETY AND REGULATIONS
Lab risks
Electrical: fire and shock Toxic vapors Compressed gasses Flammable liquids Radioactive materials ( not too common these days ) Corrosives Mechanical: moving machinery Poisons Biological: microbes, animals, plants and genetically modified agents Ergonomic: standing, repetitive motion
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Safety is…… A combination of common sense , good behavior and
housekeeping
Good personal behavior & habits such as professional conduct and appearance, frequent hand washing, no eating, smoking, drinking, or application of makeup, etc. in lab. Should also make yourself aware of emergency procedures / location and proper use of emergency equipment and fire escape route.
Good housekeeping - Laboratory cleanliness, properly labeled containers, immediate appropriate cleanup of any spills, warning signs of potential danger posted, etc.
Good laboratory technique - carefully read all instructions and labels, do not operate equipment until you are instructed and authorized to do so, always respect the power of acids, bases and corrosive chemicals. Protect yourself with PPDs and use proper eyewear protection devices.
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Precautions
No eating, drinking, application of personal products, such as make-up or contact lenses in lab area
Frequent handwashing Follow Standard precautions at all times,
treat all specimens as if infectious Never pipet by mouth Properly dispose of wastes
SAFETY AWARENESS
Safety responsibility – shared responsibility
Employer Provide and supervise safety methods, equipment and policies
Employee Understand and practice established safety procedures Notify supervisors of unsafe conditions
Bottom line … Safety is everyone’s responsibility !!!
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OSHA ( Occupational Safety and Health Act )
Federal law that mandates safe working conditions for workers
OSHA may inspect work places for compliance with safety rules
OSHA compliance is the law, not just a nice idea
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Other Regulations and Guidelines
Clean Water Act – federal
Toxic Substances Control Act – federal
JACHO (Joint Commission on Accreditation of Health Care Organizations ) – non governmental ** outlines requirements of participating healthcare agencies for accreditation.
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CHEMICAL SAFETY
Hazard Communication Standard (Right to Know Law) The lab, by law, must provide written policies for the safe use of hazardous
chemicals ( OSHA regulation ) Intent to ensure all chemical health hazards are evaluated and information
presented to employees.
Material Safety Data Sheet ( MSDS ) A list of all safety information created by the manufacturer for each laboratory
reagent (biological or chemical) The lab is responsible to collect and make available MSDS information for all
reagents and chemicals An MSDS lists general information, precautionary measures, and emergency
information. Employees must have ready access to MSDS information
Lab must have written Chemical Hygiene Plan that provides specific work practices for hazardous chemicals
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Storage and Handling of Chemicals
Different storage/handling requirements chemicals based on their differing characteristics: Flammable/Combustible - classified according to flashpoint,
among the most dangerous in the lab. Corrosives – cause injury to eyes/skin, respiratory tract if contact
is made Reactives – under certain circumstances may react violently -
require special knowledge to prevent their contact with reaction causing substances.
Carcinogenic chemicals- been determined to cause cancer. Benzidine is often used as example of lab chemical now classified as carcinogen.
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Signage and Labeling of Chemicals
Everything must be labeled !!!
Hazardous material must include all necessary safety information
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Chemical Safety
NFPA labeling system for hazardous chemicals
• The system uses a diamond-shaped symbol, four colored quadrants, and a hazard rating scale of 0 to 4.
• The health hazard is shown in the blue quadrant.
• The flammability hazard is shown in the red quadrant.
• The instability hazard is indicated in the yellow quadrant.
• The specific hazard is shown in the white quadrant.
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SAFETY EQUIPTMENT
Safety showers Eyewash stations Fire blankets Fire extinguishers Spill kits First aid kits Fume hoods Biosafety hoods Chemical storage cabinets PPEs ( Personal Protective Equipment )
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BIOLOGICAL SAFETY
Standard Precautions ( Universal Precautions ) All biological specimens must be considered potentially infectious
Always use required PPEs when handling biohazardous material
Cap specimens that are being centrifuged
Mouth pipeting is NEVER permitted under any circumstances
Spills must be cleaned using proper technique
OSHA mandates that labs provide written bloodborne and airborne ( TB ) safety policies.
Annual TB screening ( PPD test ) for hospital employee’s is mandatory
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Laboratory Safety
Cleaning Specimen Collection Areas and Biological Spills
• Wear gloves.• Use 1:10 bleach solution or commercially prepared
solution.• First clean area with visible blood and then disinfect
the entire area.• Keep the bleach in contact with contaminated area
for at least 20 minutes. • Use colored biohazard labels. • Provide accident follow-up.
Radioactivity Safety
Gamma ray - most penetrating; must store behind lead
Beta ray - less penetrating; normal storage
Must wear badge Work area monitored frequently
The Three Cardinal Principles of Self-protection
1. Time2. Shielding 3. Distance
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FIRE SAFETY
Classification of Fires Class A: Paper, wood, plastic, fabrics Class B: Flammable liquids – gasses Class C: Electrical Class D: Combustible metals
Fire extinguishers are labeled to indicate which type of fire they combat
Most extinguishers utilize a dry chemical that combats A, B and C fires
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FIRE SAFETY
Know the location of Fire alarm pull stations Extinguishers Fire blankets
Know the telephone number to report fires
RACE Rescue - Remove patients from immediate harm Alarm -Pull fire alarm Contain - Close doors and windows Extinguish - Extinguish fire
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DISPOSAL OF HAZARDOUS MATERIALS
Chemical Waste Know the SOP for the disposal of every substance You can’t assume that everything goes down the drain !
Biohazardous Waste Disposal of biohazardous waste is regulated by law Waste containers must be clearly labeled Sharps ( needles, blades, glass ) must be placed in hard containers Infectious wastes should be autoclaved before disposal General rules
Tubes and other containers of blood, papers or other materials that contain significant amounts( > 1 inch diameter on paper or ½ mL total) of blood, used culture plates should go into bio-waste
Paper towels, used gloves, KimWipes should go in regular trash
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ACCIDENT DOCUMENTATION AND INVESTIGATION
All accidents must be immediately reported to a supervisor (AFTER first aid )
Accident Investigation forms must be completed by the employee and employer
OSHA regulations require accident records to be kept 30 years
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