1 Agenda Introduction Functional traits Genomics to system biology in animal science OMICS for analyzing diseases in livestock species Genomics Transcriptomics Proteomics Conclusion Global Veterinary Summit-August 31-2 September 2015 2 Will there be enough food to support the projected population of 9.2 billion in 2050? Global Veterinary Summit-August 31-2 September 2015 3 FOOD QUALITY and SAFETY BREEDING GOALS CONSUMERS DEMANDS ENVIROMENTAL IMPACT 4 Milk yield vs reproductive traits traits? Global Veterinary Summit-August 31-2 September 2015 Data from Lucy et al, Journal of Dairy Science, 2001, 84(6): 5 Global Veterinary Summit-August 31-2 September 2015 6 What are functional traits? The ICAR* Functional Traits Working Group currently is working on: General health traits Female fertility Feet and legs problems Udder health Workability *International Commitee for Animal Recording 7 Functional traits are being used Global Veterinary Summit-August 31-2 September 2015 Source: Miglior et al., 2012 8 BREEDING GOALS ADVANTAGES REGARDING SALE OF PRODUCTS INCREASED INCOME REDUCED COSTS EASE OF MANAGE MENT Higher production of milk better fertility fewer diseases reduced culling rates Temperament Milking speed animal welfare consumer concerns Global Veterinary Summit-August 31-2 September 2015 9 10 Global Veterinary Summit-August 31-2 September Genomics to system biology in animal science 1990 QTL MAPPING PROJECTS 11 2. Genomics to system biology in animal science Genome-wide association study Genomic selection-GEBV Global Veterinary Summit-August 31-2 September 2015 12 3. Genomics to system biology in animal science Affymetrix GeneChip scanne r MICROARRAY GENE EXPRESSION PROFILING Global Veterinary Summit-August 31-2 September 2015 13 4. Genomics to system biology in animal science Global Veterinary Summit-August 31-2 September 2015 NEXT GENERATION SEQUENCING 14 Global Veterinary Summit-August 31-2 September Genomics to system biology in animal science WHAT IS THE SYSTEM BIOLOGY?????? 15 How does it integrate OMICs data and phenotypes? Global Veterinary Summit-August 31-2 September 2015 16 Application of OMICs tech for analyzing diseases in livestock species Global Veterinary Summit-August 31-2 September 2015 Diseases GenomicsTranscriptomicsMetabolomicsProteomics 17 Diseases in cattle Global Veterinary Summit-August 31-2 September 2015 18 GWAS for health Global Veterinary Summit-August 31-2 September 2015 19 GWAS for mastitis Global Veterinary Summit-August 31-2 September SIGNIFICANT SNP FOR SCS 52 WERE ANNOTATED WITHIN GENES SOME INVOLVED IN THE IMMUNE RESPONSE TO MASTITIS 20 GWAS for Johns disease in sheep Moioli, B., S. D'Andrea, L. De Grossi, E. Sezzi, B. De Sanctis, et al., 2015 Genomic scan for identifying candidate genes for Paratuberculosis resistance in sheep. Anim. Prod. Sci. in press. NEGATIVEVSPOSITIVE PARATUBERCOLOSIS ELISA TEST 30 PUTATIVE CANDITATE GENE FOR DISEASE SUSCEPTIBILITY CD109PCP4 Global Veterinary Summit-August 31-2 September 2015 21 GWAS for calving ease in cattle Global Veterinary Summit-August 31-2 September 2015 BTA6 LAP3 SHOWS THE MOST INVOLVEMENT WITH THE TRAIT 22 TRANSCRIPOTIMCS for paratubercolosis in cattle Global Veterinary Summit-August 31-2 September 2015 MICROARRAY 23 TRANSCRIPOTIMCS for paratubercolosis in cattle Global Veterinary Summit-August 31-2 September 2015 RNAseq 24 Global Veterinary Summit-August 31-2 September 2015 TRANSCRIPOTIMCS for paratubercolosis in cattle 2hp i 6hp i 25 TRANSCRIPOTIMCS for mastitis Global Veterinary Summit-August 31-2 September 2015 MICROARRAY 26 TRANSCRIPOTIMCS for mastitis RNAseq Global Veterinary Summit-August 31-2 September 2015 27 Global Veterinary Summit-August 31-2 September 2015 TRANSCRIPOTIMCS for mastitis 28 PROTEOMICS Global Veterinary Summit-August 31-2 September 2015 29 Global Veterinary Summit-August 31-2 September 2015 PROTEOMICS 30 PROTEOMICS and mastitis 95 DE proteins mammary tissuehealthy vs mastitic cow Global Veterinary Summit-August 31-2 September different glycosilated sites mammary tissuehealthy vs mastitic cow 551 N-glycosylation sites 294 proteins283 proteins HM 36 UPregulated mammary tissuehealthy vs mastitic cow 19 DOWNregulated 31 PROTEOMICS and Salmonella in pig Global Veterinary Summit-August 31-2 September 2015 32 CONCLUSIONS Global Veterinary Summit-August 31-2 September 2015 PHENOTYPES Traditional traits Functional traits BREEDING GOAL Breeder needs Consumers demands Enviromental impact OMICs Genomics Transcriptomics Proteomics Food safety and quality