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Isonet® L TT and Isonet® L TT BIONew mating disruption products
for the control of Lobesia botrana (Lepidoptera: Tortricidae)
Andrea LucchiDip. Scienze Agrarie, Alimentari e Agro-ambientali, University di Pisa
IOBC-WPRS meeting of the Working Group on "Integrated Protection and Production in Viticulture",
Austrian Agency for Health and Food Safety (AGES), Vienna, Austria
Lobesia botrana & Mating disruption
MD with hand-applied dispensers is the most well-studied and widely used pheromone-
mediated control technique against grape berry moths in Europe.
In 2015: applied on 140,000 ha in European vineyards (3–4% of the total grapevine-
growing area).
Rak, BASF Isonet, Shin-Etsu Puffer, Suterra
Lobesia botrana & Mating disruption
We have been researching on L TT and LTT BIO(biodegradable) because they show advanced merits:
- Applied at half point sources (250 vs 500)
- Applied in less time
- Biodegradable (in one case)
- Aluminum free
• reservoir pheromone dispensers: 2 parallel
capillary tubes (Twin Tubes) filled with L. botrana
pheromone blend, and joined and sealed at ends
• No need to twist, only open and hang
• gap in the middle allows each dispenser to form a
loop easily and quickly deployed over the end of
spurs or by looping it around cordons
• difference between Isonet ® L TT and Isonet ® L TT
BIO: material of which the dispensers are made:
polyethylene for Isonet ® L TT and biodegradable
plastic for Isonet ® L TT BIO
Isonet ® L TT & Isonet ® L TT BIO - products description
L TT BIO
L TT
Shape and material of which
dispensers are made is of
sound importance for a correct
and season-long release of
pheromone.
Efficacy of both products in
controlling L. botrana on
grapevine has been evaluated
and verified in several field
trials.
This talk deals with
the field research
we carried out in a
Farm of Tuscany in
2014 and
• Test site location: Bolgheri, Livorno, Tuscany (Italy)
• Grapevine cultivars: Vermentino (2014), Cabernet Sauvignon (2015)
• Tested treatments:
Isonet ® L TT & Isonet ® L TT BIO - trial design
Treatment ProductApplied rate
(dispensers/Ha)
Control -
Isonet L TT Isonet® L TT200 (2014)
250 (2015)
Isonet L TT BIO Isonet® L TT BIO200 (2014)
250 (2015)
StandardGrower’s Standard (2014)
Isonet® L (2015)
* (2014)
500 (2015)
• Grower’s standard 2014 = 2 sprayings I gen Methoxyfenozide
III gen Chlorantraniliprole
Frequency:
1. at end of 1st generation (G1; May, full flowering)
2. at end of 2nd generation (G2; July, berry touch)
3. at harvest (G3; September)
Type:
• % infested flower clusters (G1) / bunches (G2 and G3)
• N. nests per flower cluster (G1) / bunch (G2)
• N. damaged berries per bunch (G3)
10 subplots within each treatment, big enough to allow for assessments on at
least 100 bunches per subplot, were selected.
10 subplots x 100 flower clusters per treatment (G1) and 10 x 50-100 bunches per
treatment (G2 and G3).
Isonet ® L TT & Isonet ® L TT BIOASSESSMENTS
Challenges to the success of Mating Disruption:
• overcome the border effects of immigration of mated females;
• prevent potential treatment interference between plots;
• population density.
For these reasons:
• sites cannot be split into conventional plots;
• replicated treatments are often unpractical.
Plot size for the pheromone treatments may be of significant size (e.g. several hectares)
in order to achieve reliable performance. Plot size for either untreated controls or
standard insecticide treatments do not have to be as large, but should, where possible
have comparable population densities.
Whilst replication is preferred where practical, trials may be unreplicated but a suitable
number of trials should be conducted to allow appropriate support of product claims.
EPPO Guideline PP 1/264(1) Mating disruption pheromones
Similar trials in Tuscany, Emilia Romagna, Sicily and Spain supervised by the
same research team.
Statistical analysis:
• Since a randomized block design does not apply to large plots required for
studies on mating disruption products, ten subplots per each treatment
were considered as replicates;
• Data were compared across Treatments using 1-way ANOVA followed by
Student-Newman-Keuls’ test for post-hoc comparison of means. Bartlett’s
test was used to test for homogeneity of variances.
Isonet ® L TT & Isonet ® L TT BIO – data analysis
L TT BIO 1
L TT BIO 2L TT 1
L TT 2
CONTROL
Crop: grapevineVariety: Vermentino
Location:Bolgheri (LI)
Total surf.17.5 ha
L TT 5 h
L TT BIO 5 ha
Control 7.5 ha
Dosage/ha :LTT 200 d/haLTT BIO: 200 d/ha
Damage assessment:
I° gen: 31 May
II° gen: 17 July
III° gen: 03 Sept
1,000 clusters/plot (G1)
500 clusters/plot (G2 + G3)
WIND
BOLGHERI PLOT 2014
1
32
111
1
5
4
Grower’s standard
Experimental area
0
10
20
30
40
50
60
70
18.3
25.3
1.4
8.4
15.4
22.4
29.4
6.5
13.5
20.5
27.5
3.6
10.6
17.6
24.6
1.7
8.7
15.7
22.7
29.7
5.8
12.8
19.8
26.8
2.9
9.9
average captures/trap
NO
MD
20
14
NO
MD
1 2
01
4
NO
MD
trap
cap
ture
sB
OLG
HE
RI P
LOT
20
14
L TT
BIO
1
Trap
cap
ture
sB
OLG
HE
RI P
LOT
20
14
Re
sults
20
14
0 2 4 6 8
10
12
14
16
24.3
31.3
7.4
14.4
21.4
28.4
5.5
12.5
19.5
26.5
2.6
9.6
16.6
23.6
30.6
7.7
14.7
21.7
28.7
4.8
11.8
18.8
25.8
1.9
average captures/trap
L TT
L TT
BIO
Gro
we
r's Sta
nd
ard
Co
ntro
l
ResultsBOLGHERI PLOT 2014
Results 2014
0,0
5,0
10,0
15,0
20,0
25,0
30,0
35,0
40,0
45,0
50,0
CONTROL L TT L TT BIO GROWER'S
STAND.
5,3 b
1,3 c 1,2 c
15,9 a
31,2 a
13,0 b9,8 b
26,6 a
22,2 a
6,4 b4, 2 b
19,8 a
% Infested flower clusters (G1) / bunches (G2 and G3)
G1
G2
G3
Grower’s standard was sprayed with Prodigy (Methoxyfenozide, 400 ml/ha) against G1 and
all fields were sprayed with Coragen (Chlorantraniliprole, 150 mL/ha) against G3
ResultsBOLGHERI PLOT 2014
Results 2014
0,00
0,05
0,10
0,15
0,20
0,25
0,30
0,35
0,40
0,45
0,50
CONTROL L TT L TT BIO GROWER'S
STAND.
0,05 b
0,014 c0,015 c
0,186 a
0,42 a
0,136 b0,108 b
0,326 a
0,432 a
0,082 b0,048 b
0,282 a
N. nests (G1 and G2) and
damaged berries (G3) per bunch
G1
G2
G3
Grower’s standard was sprayed with Prodigy (Methoxyfenozide, 400 ml/ha) against G1 and
all fields were sprayed with Coragen (Chlorantraniliprole, 150 mL/ha) against G3
Grower’s standard was sprayed with Prodigy (Methoxyfenozide, 400 ml/ha) against G1 and
all fields were sprayed with Coragen (Chlorantraniliprole, 150 mL/ha) against G3
Grower’s standard was sprayed with Prodigy (Methoxyfenozide, 400 ml/ha) against G1 and
all fields were sprayed with Coragen (Chlorantraniliprole, 150 mL/ha) against G3
Migliarini stradone
Cbs
56,8%
Migliarini 2001
Vm
55,20%
Pozzi al comune
Vm19,8%
Campone
Cbs
5,6%
Camillo
LTT1
LTT2BIO1
BIO2
% INFESTATION III GENERATION 2014 DESPITE 2 SPRAYINGS
WITH METHOXYFENOZIDE AND CHLORANTRANILIPROLE
Bettelli
Cbs
Belvedere
Cbs
S.Antonio
Mer
2,9%
2014 trial
2015 trial
Crop: grapevineVariety: Cab. Sauvign. Location: Bolgheri (LI)
Total surface: 25 ha
Isonet L TT: 8.5 haIsonet L TT BIO: 7.8 haIsonet L : 7.2 haControl: 1,5 ha
Dosage/ha :
Isonet L TT: 250 d/haIsonet L TT BIO: 250 d/haIsonet L: 500 d/ha
Damage assessment:I° gen: 27 MayII° gen: 16 JulyIII° gen: 03 Sept
1,000 clusters/plot (1G)500 clusters/plot (2G + 3G)
BOLGHERI PLOT 2015
L TT 1
L TT 2
L TT BIO 1
L TT BIO 2
L 1
L 2
CONTROL
0
20
40
60
80
10
0
12
0
18.3
25.3
1.4
8.4
15.4
22.4
29.4
6.5
13.5
20.5
27.5
3.6
10.6
17.6
24.6
1.7
8.7
15.7
22.7
29.7
5.8
12.8
19.8
26.8
average captures/trap
NO
MD
20
15 N
O M
D tra
pca
ptu
res
BO
LGH
ER
I PLO
T 2
01
4-2
01
5
Trap
cap
ture
sB
OLG
HE
RI P
LOT
20
15
Re
sults
20
15
0 5
10
15
20
25
30
35
30.3
6.4
13.4
20.4
27.4
4.5
11.5
18.5
25.5
1.6
8.6
15.6
22.6
29.6
6.7
13.7
20.7
27.7
3.8
10.8
17.8
average captures/trap
L TT
L TT
BIO
LCo
ntro
l
ResultsBOLGHERI PLOT 2015
0,0
5,0
10,0
15,0
20,0
25,0
30,0
35,0
40,0
45,0
50,0
CONTROL L TT L TT BIO L
5,9 a
0,8 b 0,8 b 1,3 b
25,5 a
2,3 b 3,1 b5,1 b
19,2 a
2,0 c
8,6 b 7,8 b
% infested flower clusters (G1) / bunches (G2
and G3)
G1
G2
G3
ResultsBOLGHERI PLOT 2015
Results 2015
0,00
0,05
0,10
0,15
0,20
0,25
0,30
0,35
0,40
0,45
0,50
CONTROL L TT L TT BIO L
0,071 a
0,009 b 0,009 b 0,014 b
0,287 a
0,023 b 0,031 b0,051 b
0,474 a
0,034 c
0,222 b
0,172 b
N. nests (G1 and G2) and
damaged berries (G3) per bunch
G1
G2
G3
• Time required for application of L TT dispensers was 1 hour per ha compared
with 1.5 hs per ha of Isonet L
• In both years, Isonet® L TT and Isonet® L TT BIO resulted in almost complete
trap catches shut-down of L. botrana.
• G1, G2 and G3 L. botrana infestation was lower in Isonet ® L TT and Isonet ® L TT
BIO than in the control treatment.
• Efficacy of both products in controlling L. botrana was comparable to or higher
than that of the reference treatment. This was confirmed by the results
obtained in other Italian Regions and in Spain.
• Isonet® L TT and Isonet® L TT BIO can be considered valuable tools for effective
and less labour-intensive control of European grapevine moth.
Isonet ® L TT & Isonet ® L TT BIO trials
Summary