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How to successfully handle pets’ aging process?

© 2019 SPF- DPF all rights reserved© 2019 SPF- DPF all rights reserved

Anne LepoudèreR&D Manager, VIVAE – Diana Pet Foodalepoudere@diana-petfoodcom

Fernando de OliveiraDVM, R&D Project Manager, VIVAE – Diana Pet Food

Background

How to successfully handle pets’ aging process?

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Background

Understanding what Ageing means

Nutritional approach: Focus on Polyphenols

Key home messages

Context

For owners, the question raised is no longer “Is my pet going to live longer” but “Is my pet going to remain healthy as it ages ”?

Need for comfort is in the top 5 consideration of Caring for Geriatric Patients

Pet healthy aging is a growing concern for pet pare nts

Dogs and cats’ lifespan is continuously increasing

Lifespan increase2002-2012

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Senior pet food offer is on the riseMore than 45 M dogs around 7-years-old

in US & EU Bosch, 2012

2002-2012

+ 10% + 4%

“ “

Banfield State of Pet Health 2013 report

Rachel V. Poulin, Coral Springs Animal Hospital, 2015

Factors impacting health and longevity

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O’Neill et al. (2013); Kaeberlein et al. (2016); Creevy et al. (2016); Salt et al. (2018)

Average lifespan

Average weight

European cat12 yrs

4-5 kg45- 90 kg 23 - 41 kg 14-18 kg 2-3 kg

Aging from a biological stand point

Biological process common to all living organisms, defined as the progressive reduction of its ability to face the demands of the environment. It is influenced by genes, the environment and the interaction between both. Butterwick, 2016 ; Creevy 2018

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Process characterized by progressive loss of tissue and organ function.Liguori et al., 2018

Declining ability to respond to stressful situations, increased homeostatic imbalance and increased risk of diseases.Alexander et al., 2017

Biological alterations due to agingMOLECULAR CELLULAR

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PHYSIOLOGICAL

Kunlin Jin, 2010; Marsman et al., 2018

FUNCTIONAL

Dysregulation of immune & inflammatory responses

Ageing is associated with dysregulated immune and inflammatory responsesHyperactivity of innate immune responseHypoactivity of cell mediated immunity

Immunosenescence

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Multifactorial complex of changes that occur in the immune system with age.

Inflammaging

Age-related constant challenge associated with the production of inflammatory mediators.

Day 2010; Alexander et al., 2017; Marsman, 2018

Adapted from Yin et al., 2013; Liguori et al. 2018

Oxidative stress: biological effects of ROS

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Chronic diseases linked with oxidation & inflammation OXIDATIVE

STRESSOsteoarthritis

Diabetes

Cancer

Chronic kidney diseases

Neurodegenerativediseases

Chronic obstructive pulmonary diseases

Cardiovascular

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Adapted from Liguouri et al. (2018)Ferrucci & Fabbri (2018) &

Gut microbiota

Existing diseases:e.g. Obesity

Genetic predisposition

Chronic infections

External actors(pollution, UV, etc.)

Psychological Stress

STRESS

INFLAMMATION

Osteoarthritis Cardiovasculardiseases

What Polyphenols can do for pets?

Against oxidative stress•ROS scavenging•Effect on enzymes implied in ROS production•Protection of cell membrane proteins and lipids•Inhibition of enzymes involved in oxidation

Secondary metabolites synthesized by plants,with phenolic structural units, hydroxyl groupsSarni-Machado & Cheynier, 2006; Hussain 2016

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Against inflammation•Effect on enzymatic and signaling systems involved in inflammatory processes•Modulation of pro-inflammatory molecules

Naringin

Polyphenols bioavailability and antioxidant properti es can be improved when they are combined .

Effect of a polyphenols combination on oxidative stress and inflammation in adult dogs

30 Beagle dogs

1 to 12 years old 15 dogs : Control diet

15 dogs : Diet supplemented

with 500 ppm polyphenols blendCONTROL DIET

Blend of polyphenols from Rosmarinus sp., Syzygium sp.,Curcuma sp. and Citrus sp

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76542 31

PRE-SUPPLEMENTATIONTraining to feeding conditions

TRANSPORT

CONTROL DIET

Measurement of

- Lipid Peroxidation biomarker : Tbars

- Inflammation biomarker : CRP

Measurement of

Lipid Peroxidation

biomarker : Tbars

8 9 10 11

SUPPLEMENTATION

Weeks

40

50

60

70

80

TBARS

TBARS decreased in the supplemented group at the end of the trial but increased in the control group

Impact of a polyphenols combination on lipid peroxidation (TBARS) before transportation stress

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-30

-20

-10

0

10

20

30

40

CONTROL PRO'AGE

δ [D28 - D0] (%)

the trial but increased in the control group(normal conditions).

The polyphenols combination could prevent and decrease the lipid peroxidation in a normal conditio n

0

10

20

30

CONTROL PRO'AGEδ [One-hour

CRP

Impact of a polyphenols combination on inflammation (CRP) after transportation stress

CRP (C-Reactive Protein) increased in the control group and not in the supplemented group after transportation.

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-50

-40

-30

-20

-10

CONTROL PRO'AGEδ [One-hour

post stress -

D28] (%)

transportation.

Potential anti-inflammatory effect of the polypheno lcombination

Aging process induces numerous changes at the cellular and metabolic levels, leading to the development of various chronic diseases

Nutritional strategies focusing on the limitation of

Take home messages

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oxidative stress and inflammation may help maintain old pets in good health

Natural plant-based ingredients represent an attractive solution to limit age-related effects in dogs

THANK YOU

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THANK YOUFOR YOUR ATTENTION