View
720
Download
2
Category
Preview:
Citation preview
MCQ009 Savepaper…Savetrees…
FormoreQuestionsandResourcesforyourCSIR/ICMR/DBT/ICAR/UGC/JRFNETLifeScienceExaminationpleasevisit:
www.easybiologyclass.com 1
BiologyMCQ(MultipleChoiceQuestionsinLifeScience)(Sample/Model/PracticeQuestionsforJRF/NETLifeScienceExamination,ICMRJRF,DBTJRF,GATE,ICARNET,PGEntrance)
BiophysicalInstrumentation:SeparationTechniquesPart1:(MCQ009)
1. Whenvoltage‘V’isappliedacrossapairofelectrodes(cathodeandanode),apotentialgradient‘E’iscreatedbetweentheelectrodes.Wecancalculate‘E’as:
a. E=V/db. E=(1/V)xqc. E=(Vd)/qd. E=V+d
2. Thevelocity(‘v’)ofachargedparticlesinanelectricfieldinamediumcanbemathematicallyexpressedasv=Eq/f,where‘Eq’and‘f’are________.
a. Eq:Energy;f:Frictionalforceb. Eq:Electricalforce;f:Gravitationalforcec. Eq:Electricalforce;f:Frictionalco‐efficientd. Eq:Equilibriumconstant;f:co‐efficientofgravity
3. FortheseparationofDNAbyelectrophoresis,whichofthefollowingmethodiscommonlyused?
a. Agarose‐verticalb. Agarose‐horizontalc. PAGE‐verticald. PAGE‐horizontal
4. Sodiumdodecylsulfate(SDS)usedinSDSPAGEis___________.a. Ananionicdetergentb. Acationicdetergentc. Anon‐ionicdetergentd. Ananionexchangere. Acationexchanger
5. Functionofβ‐mercaptoethanolinSDS‐PAGEis__________.a. Togivenegativechargestoaminoacidsintheproteinsb. Fortheoxidationofdisulfidebondsintheproteinsc. Forthereductionofdisulfidebondsintheproteinsd. Forbreakinghydrogenbondsintheproteins
www.easy
biolog
yclas
s.com
MCQ009Savepaper…Savetrees…
FormoreQuestionsandResourcesforyourCSIR/ICMR/DBT/ICAR/UGC/JRFNETLifeScienceExaminationpleasevisit:
www.easybiologyclass.com 2
6. Theratioofvelocity(‘v’)ofbiomoleculeinamediumunderconstantelectricfield(‘E’)iscalled‘Electrophoreticmobility’denotedas‘µ’.‘µ’ismathematicallyexpressedas:
a. µ=E/vb. µ=v/Ec. µ=1/(Ev)d. µ=VE
7. Inelectrophoresis,theelectrophoreticmobility(‘µ’)isdeterminesthecharacteristicsofmigrationofdifferentbiomolecules.Whichofthefollowingisnothavinganyinfluencein‘µ’?
a. Stereochemistryofmoleculeb. Sizeofmoleculec. Shapeofmoleculed. Molecularweighte. Netchargeofmolecule
8. Electrophoresisisnotusedfortheseparationof________.a. Nucleicacidsb. Proteinsc. Aminoacidsd. Lipids
9. InSDS‐PAGEofproteinseparation,oneSDSmoleculewillbindsto__________.a. Everyaminoacidb. Everytwoaminoacidsc. Everythreeaminoacidsd. EveryFouraminoacids
10. InSDS‐PAGE,migrationofproteiniseffectedby____________.a. Chargeofproteinb. Sizeofproteinc. Netchargeofproteind. Allofthese
11. Innative‐PAGEtheseparationofproteinisinfluencedby___________.a. Chargeofproteinb. Sizeofproteinc. pIofproteind. Both(a)and(b)e. Both(b)and(c)
12. ThemainadvantageofdiscontinuousbuffersysteminSDSandNativePAGEis________.www.ea
sybio
logyc
lass.c
om
MCQ009Savepaper…Savetrees…
FormoreQuestionsandResourcesforyourCSIR/ICMR/DBT/ICAR/UGC/JRFNETLifeScienceExaminationpleasevisit:
www.easybiologyclass.com 3
a. Conformationofproteinisconservedb. Constantlymaintainthechargeofproteinsc. Assistinmigrationofproteind. Enhanceresolutionofseparation
13. ThepHof(i)stacking,(ii)resolvinggeland(iii)tankbufferinSDSPAGEis_________respectively.
a. (i).8.30 (ii)8.80 (iii)6.80b. (i).6.80 (ii)8.80 (iii)8.30c. (i).8.30 (ii)6.80 (iii)8.80d. (i).6.80 (ii)8.30 (iii)8.80
14. ThepercentageofSDScommonlyusedinthebuffersofSDSPAGEis__________.a. 0.1%b. 1.0%c. 10%d. 1–10%
15. TheroleofureainPAGEseparationofDNAisto_______.a. Actasanionb. Actascationc. HelpstodenaturetheDNAd. Providebufferstabilityofthegel
16. TheroleofAPSinSDSPAGEisto________.a. Actasacatalystinthepolymerizationofacrylamideb. Actasasourceoffreeradicalsc. Actasabridgebetweenacrylamideandbis‐acrylamided. Actasaporebuilderinthepolymerizedgel
17. The‘trackingdye’usedinSDSPAGEwillbe______.a. Anionicb. Cationicc. Non‐ionicd. Amphipathic
18. Whichofthefollowingisusedasa‘trackingdye’inSDS‐PAGEofprotein?a. Bromophenolblueb. Xylenecyanolc. OrangeGd. Both(a)and(b)e. Allofthese
19. Glycerolisaddedtoproteinsamplesbeforetheyareloadedtothe‘wells’ofPAGE.Thefunctionofglycerolisto_______.www.ea
sybio
logyc
lass.c
om
MCQ009Savepaper…Savetrees…
FormoreQuestionsandResourcesforyourCSIR/ICMR/DBT/ICAR/UGC/JRFNETLifeScienceExaminationpleasevisit:
www.easybiologyclass.com 4
a. Stabilizeproteinstructureb. Providedensitytothesamplec. HelpstobindSDStotheproteind. Helpstoreducedisulfidebondsbyβ‐mercaptoethanol
20. ForthebetterresolutionofminuteproteinbandsinSDS‐PAGE,whichofthefollowingstainingmethodisadvised?
a. CBBStainingb. Silverstainingc. Avidinstainingd. Allofthese
Forcorrectanswersandexplanationsclickbelow(http://www.easybiologyclass.com/biology‐mcq‐multiple‐choice‐questions/
Visitwww.easybiologyclass.comformore….
www.easy
biolog
yclas
s.com
Recommended