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BiobrickBiobrickAn adjective, not a noun
http://en.wikipedia.org/wiki/Biobrick
OverviewOverview• Short introduction to biobrick parts and plasmids• How to make plasmids with biobrick parts• How to combine the biobrick parts in one plasmid
Biobrick part in a Biobrick part in a plasmidplasmid
http://partsregistry.org/Help:An_Introduction_to_BioBricks
The plasmid The plasmid (backbone)(backbone)
http://partsregistry.org/Help:Plasmids
E EcoRI
X XbaI
S SpeI
P PstI
http://partsregistry.org/Help:Plasmid_backbones/Glossary
Construction plasmidsConstruction plasmids
http://partsregistry.org/Part:BBa_P1010
Prefix & SuffixPrefix & SuffixPrefix: GAATTCGCGGCCGCTTCTAGAG
CTTCCGCGCCGGCGAAGATCTC
Suffix: TACTAGTAGCGGCCGCTGCAGATGATCATCGCCGGCGACGTC
EcoRIXbaISpeIPstINotIPrefix cut with
XbaI:...GCTT^CTAG AG...CGAA GATC^TC
Suffix cut with SpeI:TA^CTAG TAGC...AT GATC^ATCG...
SpeI + XbaI...TA^CTAG AG......AT GATC^TC...
http://partsregistry.org/Help:BioBrick_Prefix_and_Suffixhttp://partsregistry.org/Help:Plasmid_backbones/Glossary
Standard assemblyStandard assembly
http://partsregistry.org/Help:Standard_Assembly_%28zoom%29
RBS-CDS issuesRBS-CDS issues
http://partsregistry.org/Assembly:RBS-CDS_issues
XbaI + SpeI(RBS)TA^CTAG AG(CDS)(RBS)AT GATC^TC(CDS)
Scars:Normal:(RBS)TACTAGAG(CDS) Scar of 8 basesWith T:(RBS)TACTAGATG(CDS-ATG) Scar of 6 bases
Parallel/Rolling Parallel/Rolling assemblyassembly
http://partsregistry.org/Assembly:Rolling_assembly
Robotic assemblyRobotic assembly
epMotion5075
http://partsregistry.org/Assembly:Robotic_assembly
QuestionsQuestions
http://www.firstnews.co.uk/bored/can-you-do-this-word-search-i1919
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