BiobrickBiobrickAn adjective, not a noun

http://en.wikipedia.org/wiki/Biobrick

OverviewOverview• Short introduction to biobrick parts and plasmids• How to make plasmids with biobrick parts• How to combine the biobrick parts in one plasmid

Biobrick part in a Biobrick part in a plasmidplasmid

http://partsregistry.org/Help:An_Introduction_to_BioBricks

The plasmid The plasmid (backbone)(backbone)

http://partsregistry.org/Help:Plasmids

E EcoRI

X XbaI

S SpeI

P PstI

http://partsregistry.org/Help:Plasmid_backbones/Glossary

Construction plasmidsConstruction plasmids

http://partsregistry.org/Part:BBa_P1010

Prefix & SuffixPrefix & SuffixPrefix: GAATTCGCGGCCGCTTCTAGAG

CTTCCGCGCCGGCGAAGATCTC

Suffix: TACTAGTAGCGGCCGCTGCAGATGATCATCGCCGGCGACGTC

EcoRIXbaISpeIPstINotIPrefix cut with

XbaI:...GCTT^CTAG AG...CGAA GATC^TC

Suffix cut with SpeI:TA^CTAG TAGC...AT GATC^ATCG...

SpeI + XbaI...TA^CTAG AG......AT GATC^TC...

http://partsregistry.org/Help:BioBrick_Prefix_and_Suffixhttp://partsregistry.org/Help:Plasmid_backbones/Glossary

Standard assemblyStandard assembly

http://partsregistry.org/Help:Standard_Assembly_%28zoom%29

RBS-CDS issuesRBS-CDS issues

http://partsregistry.org/Assembly:RBS-CDS_issues

XbaI + SpeI(RBS)TA^CTAG AG(CDS)(RBS)AT GATC^TC(CDS)

Scars:Normal:(RBS)TACTAGAG(CDS) Scar of 8 basesWith T:(RBS)TACTAGATG(CDS-ATG) Scar of 6 bases

Parallel/Rolling Parallel/Rolling assemblyassembly

http://partsregistry.org/Assembly:Rolling_assembly

Robotic assemblyRobotic assembly

epMotion5075

http://partsregistry.org/Assembly:Robotic_assembly

QuestionsQuestions

http://www.firstnews.co.uk/bored/can-you-do-this-word-search-i1919