The inferior olivary nucleus is the only source of cerebellar climbing fibers Introduction to...

The inferior olivary nucleus is the only source of cerebellar climbing fibers

Introduction to NeurobiologyLecture 10: The inferior olive 1

The Inferior Olive nucleus 2

Olivary neurons (Ramon Y Cajal)

3

Intracellular labeling of olivary neuron HRP filled olivary neuron4

Action potential in olivary neuron5

Action potentials in olivary neuron6

The effect of TTX on action potentials in olivary neuron7

The effect of Mn2+ and Cd2+ on action potentials in olivary neuron 8

-68 mV

-60 mV

-60 mV

-55 mV

Two types of action potentials in olivary neuron

20 msec

1 nA

15 mV 15 mV

-60 mV

9

The Na+ and Ca2+ components of the two types of action potentials

10

TTX

Independent activation of the action potentials 11

Refractory period of the action potentials 12

10 mV

Refractory period of the action potentials 13

The Inactivation curve of the low threshold action potential14

The effect of Harmaline on Olivary neuron . 15

The effect of harmaline on the Inactivation curve .

control

harmaline

control

harmaline

Resting potential

16

The effect of Octanol on Olivary neuron . 17

Possible mechanism of rhythmic activity

Ionic currents in Olivary neurons:

Na current – typical H&H current

Dendritic Ca current – high threshold non-inactivating

Ca dependent, K current

Somatic Ca current – low threshold inactivating

18

Cell 1 Cell 2

Cell 1

Cell 2

10 mV

5

200 ms

10 mV

20 mV

Cell 1

Cell 25

mV

200 ms

Cell 1

Cell 22

mV

100 ms

20 mV

Electrical coupling between olivary neuron 19

Dye coupling between olivary neuron 20

The olivary glumerulus 21

Olivary Gap junction 22