Embed Size (px)
Citation preview
The inferior olivary nucleus is the only source of cerebellar climbing fibers
Introduction to NeurobiologyLecture 10: The inferior olive 1
The Inferior Olive nucleus 2
Olivary neurons (Ramon Y Cajal)
3
Intracellular labeling of olivary neuron HRP filled olivary neuron4
Action potential in olivary neuron5
Action potentials in olivary neuron6
The effect of TTX on action potentials in olivary neuron7
The effect of Mn2+ and Cd2+ on action potentials in olivary neuron 8
-68 mV
-60 mV
-60 mV
-55 mV
Two types of action potentials in olivary neuron
20 msec
1 nA
15 mV 15 mV
-60 mV
9
The Na+ and Ca2+ components of the two types of action potentials
10
TTX
Independent activation of the action potentials 11
Refractory period of the action potentials 12
10 mV
Refractory period of the action potentials 13
The Inactivation curve of the low threshold action potential14
The effect of Harmaline on Olivary neuron . 15
The effect of harmaline on the Inactivation curve .
control
harmaline
control
harmaline
Resting potential
16
The effect of Octanol on Olivary neuron . 17
Possible mechanism of rhythmic activity
Ionic currents in Olivary neurons:
Na current – typical H&H current
Dendritic Ca current – high threshold non-inactivating
Ca dependent, K current
Somatic Ca current – low threshold inactivating
18
Cell 1 Cell 2
Cell 1
Cell 2
10 mV
5
200 ms
10 mV
20 mV
Cell 1
Cell 25
mV
200 ms
Cell 1
Cell 22
mV
100 ms
20 mV
Electrical coupling between olivary neuron 19
Dye coupling between olivary neuron 20
The olivary glumerulus 21
Olivary Gap junction 22
