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StaphylococcusStaphylococcus aureus, Staphylococcus epidermidis, and Staphylococcus saprophyticuswww.easymicrobiology.com
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Staphylococcus
Kingdom: Bacteria
Phylum: Firmicutes
Class: Bacilli
Order: Bacillales
Family:Staphylococcaceae
Genus: Staphylococcus
Dr.Aravind
• Gram positive cocci in clusters or groups.
• Catalase positive
• Facultative anaerobes
• Oxidase-negative
• Medically important Organisms:• Staphylococcus aureus,
• staphylococcus epidermidis,
• staphylococcus saprophyticus.
Dr.Aravind
Catalase Test
Dr.Aravind
Staphylococcus
Dr.Aravind
Staphylococcus classification
StaphylococcusCatalase +, clusters
S.aureusβ hemolytic,
yellow on Blood
agar, Mannitol
positive.
Non-Coagulase
S.epidermidisNovobiocin sensitive,
β or γ Hemolytic
S.SaprophyticusNovobiocin resistant,
γ hemolytic
Coagulase Positive
Dr.Aravind
Staphylococcus aureus
• Coagulase positive
• Golden yellow colony on blood agar
• Nonmotile, Nonsporeforming
• Facultative anaerobe
• Fermentation of glucose produces mainly lactic acid
• Ferments mannitol (distinguishes from S. epidermidis)
Mannitol fermentation
Dr.Aravind
To perform the Slide Coagulase Test, place a 2ml of coagulase reagent (rabbit plasma) onto a clean test tube, and then add several colonies of the unknown Staphylococcus. Mix well.
If fine grains of sand or small clumps are seen, the coagulase test is positive.If the mixture remains smooth (milky looking), the coagulase test is negative. Courtesy: http://staff.um.edu.mt.
Staphylococcus aureus
Dr.Aravind
Virulent FactorsAdhesion:
Clumping factor - surface proteins
• Attaches to laminin, fibronectin, fibrin and fibrinogen, collagen (osteomyelitis)
Invasion:
alpha toxin (alpha - hemolysin)
membrane-damaging toxin (platelets and monocytes)
ß-toxin (sphingomyelinase) – β-hemolysis.
delta toxin – detergent like (produced by s. aureus and s.epidermidis)
Leukocidin – acts on PMN’s (↑ dermatonecrotic strains)
Coagulase extracellular protein binds to prothrombin → staphylothrombin → inhibits Phagocytosis
Staphylokinase, proteases, a lipase, (DNase) and a fatty acid modifying enzyme (FAME) important in abscesses
Dr.Aravind
Exotoxins
Enterotoxin – leads emesis ( food poisoning in 2-6 hrs)
TSST-1 (superantigen) – Toxic shock syndrome
Exfoliatin toxin – Scalded skin syndrome (Cadherins in desmosomes)
Pencillinase
Host Defenses escape
Capsular Polysaccharide
Protein A
Surface protein binds IgG molecules by their Fc region ↓ opsonization.
Dr.Aravind
Super antigen animation
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Virulent Factors
Courtesy : Dr.Kenneth Toder
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Epidemiology
Most opportunistic Pathogen
Nosocomial
Skin, mucus membrane and nasal areas.
Food, tampons, trauma, immune suppression etc
Dr.Aravind
Dr.Aravind
Skin Infections:
Invasion: Leukocidin,
hemolysins.
Pneumonia:Invasion: hemolysins, Leukocidin and coagulase.
Endocarditis:
Invasion:
Coagulase, staphylokinase,
hemolysins and leukocidins.
Osteomyelitis & septic
arthritis:
Invasion:
Surface proteins to
collagen, hemolysins and
leukocidin
Toxic Shock Syndrome:
TSST 1, Enterotoxin.
Food Poisoning:
Enterotoxin
Scalded skin syndrome:
Exfolitin toxin
Pathogenesis
Dr.Aravind
Clinical featuresSkin conditions:
Folliculitis, Carbuncle, furuncle, Impetigo, Mastitis,Surgical infections
Opportunistic, immune suppression.
Pneumonia:Fever, chills, cough, consolidation, effusion
Hospitalized pts, post influenza infection, immune suppressed pts.
Acute Endocarditis:IV drug abuse, tricuspid valve, pneumonia
Osteomyelitis & Septic arthritis:Most common, trauma or hematogenous
Food poisoning:
2-6 hrs onset, resolve in 24-48 hrs.Mayonnaise, salads etc
Toxic shock syndrome:Tampons, sutures, Endometritis etc.
Scalded skin syndrome:Children with skin infections and umbilical cord infections.Dr.Aravind
furuncle
Impetigo Scalded skin syndrome Folliculitis
Carbuncle
Courtesy by CDC
Toxic shock syndrome
Dr.Aravind
Diagnosis Gram staining
Catalase test
Coagulase test
Yellow colonies on blood agar
PYR +
Bacitracin resistant.
Dr.Aravind
Gram Positive cocci Identification
Dr.Aravind
• Treatment:
• Resistance to penicillin
• Vancomycin drug of choice
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MRSA
Resistant to Antibiotics
HA MRSA
CA MRSA
Susceptibility – hospitalized patients, immune suppressed, improper hygiene, athletes etc.
Treatment: Vancomycin.
Dr.Aravind
Staphylococcus epidermidis • Gram +, in clusters can’t differentiate in gram stain with S.aureus.
• Coagulase negative
• Mannitol negative
• Thick polysaccharide capsule, delta toxin.
• Most common on skin flora
• Common contaminant of blood samples
• Nosocomial
Dr.Aravind
Virulent factors:
• Polysaccharide capsule
• Delta toxin
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• Clinical conditions :
• Nosocomial infections
• Catheters and prosthetic devices
• Endocarditis of prosthetic heart valves
• Infections of artificial joints
Dr.Aravind
Diagnosis
• Coagulase negative
• Mannitol negative
• Beta or gamma hemolytic on blood agar
• Novobiocin sensitive
Dr.Aravind
Treatment
• Resistant to antibiotics
• Vancomycin
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Staphylococcus saprophyticus
• Gram positive cocci inclusters
• Non hemolytic
• Coagulase negative
• Mannitol negative
• Novobiocin resistant
• UTI in sudden sexually active women (honey moon cystitis)
Dr.Aravind
Diagnosis
• Novobiocin resistant
• Coagulase negative
• Mannitol negative
Treatment
• Penicillin
Dr.Aravind
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