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Dietary Polyphenols&
Nutrient TransportDr. Fawaz Alzaid
Background
• B.Sc. (Hons) in Human Nutrition– University of Westminster
• M.Sc. Nutrition, Physical Activity & Public Health– University of Westminster
• Ph.D. Diabetes & Nutritional Sciences– King’s College London
Dietary polyphenolic compounds influence nutrient transport processes in intestinal
epithelial cells
Elucidating mechanisms to advise nutraceutical applications
BioinformaticsGO clustering
Molecular function Biological process
2557 genes differentially expressed with polyphenol treatment [↓1796; ↑761]
BioinformaticsFunctional Similarity Clustering
Bait gene
Nutrient pathways affected by dietary polyphenols
Glucose
Iron
Copper
Nutrient pathways affected by dietary polyphenols
Glucose
Iron
Copper
Polyphenols & glucose uptake
Transport componentGLU
T2
Glucose
Circulation
Apical
Basolateral
SGLT1
Sugar pool
GLUT2
Polyphenols & glucose uptake
GLUT2
Glucose
Circulation
Apical
Basolateral
SGLT1
Sugar pool
GLUT2
GLUT2
Glucose
Circulation
Apical
Basolateral
SGLT1
Sugar pool
GLUT2
Polyphenols & glucose uptake
GLUT2
Glucose
Circulation
Apical
Basolateral
SGLT1
Sugar pool
GLUT2
Polyphenols & glucose uptake
GLUT2
Glucose
Circulation
Apical
Basolateral
SGLT1
Sugar pool
GLUT2
Control Berry extract
GLUT2
β-actin
SGLT1
β-actin
Polyphenols & glucose uptake
p<0.05
NS
Nutrient pathways affected by dietary polyphenols
Glucose
Iron
Copper
DCYTB
Fe pool
FPN HEPH
DMT1
Fe3+ Fe2+
Fe2+ Fe3+ Circulation
Apical
Basolateral
Polyphenols & iron transport
55Fe
upt
ake
55Fe
Tra
nsep
ithel
ial fl
ux (%
)
DCYTB
Fe pool
FPN HEPH
DMT1
Fe3+ Fe2+
Fe2+ Fe3+ Circulation
Apical
Basolateral
Polyphenols & iron transport
DCYTB
Fe pool
FPN HEPH
DMT1
Fe3+ Fe2+
Fe2+ Fe3+ Circulation
Apical
Basolateral
Polyphenols & iron transport
DCYTB
Fe pool
FPN HEPH
DMT1
Fe3+ Fe2+
Fe2+ Fe3+ Circulation
Apical
Basolateral
Polyphenols & iron transport
NS
NS
Nutrient pathways affected by dietary polyphenols
Glucose
Iron
Copper
Polyphenols & copper uptake
DCYTBSTEAP
CTR1
Cu2+ Cu+
Circulation
Apical
Basolateral
DMT1
ATP7B ATP7A
HAH1
?
?
Polyphenols & copper uptake
DCYTBSTEAP
CTR1
Cu2+ Cu+
Circulation
Apical
Basolateral
DMT1
ATP7B ATP7A
HAH1
?
?
Polyphenols & copper uptake
DCYTBSTEAP
CTR1
Cu2+ Cu+
Circulation
Apical
Basolateral
DMT1
ATP7B ATP7A
HAH1
?
?
Polyphenols & copper uptake
DCYTBSTEAP
CTR1
Cu2+ Cu+
Circulation
Apical
Basolateral
DMT1
ATP7B ATP7A
HAH1
?
?
NS
NS
Dietary Polyphenols & Nutrient Transport• Dietary polyphenols affect a number of nutrient
transport pathways: Glucose, Fe, Cu transport• Glucose uptake:
– Acute inhibition– Transcriptional &translational effect. No functional inhibition
• Iron transport: – Acute inhibition– Transcriptional effect & functional inhibition
• Copper uptake:– Acute transporter-specific enhancement– Transcriptional effects & transporter-specific enhancement
Thank youDr. Fawaz Alzaid
FPN
DMT1
ACTIN
GLUT2
SGLT1
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