Microscope & Simple Epithelium

Microscope & Simple Epithelium

LAB EXERCISEBY

DR. ANSARIWednesday, May 3, 2023

05/03/23 1HISTOLOGY LAB.1.

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OBJECTIVES

• Parts of a compound microscope.• Function of each part.• Types of microscope.• Types of tissues• Features of simple epithelium• Types of simple epithelium• Locations of simple epithelium

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PARTS OF A COMPOUNDMICROSCOPE

EYE PIECE

• The part of the microscope that you look through. Eyepieces in most compound microscopes have a lens with a 10x magnification level. Our Advanced microscope has a pointer built in, which appears as a black line across half of the field of view. To move the pointer, just turn the top of the eyepiece.

05/03/23 The eye piece/ocular 5

The first set of objectives are called low power, The magnification range is 10X; 4X

05/03/23 The objectives lenses 6

The second set of objectives are called “high Power”

They multiply 40X; & 100X. 100X is oil immersion

05/03/23 THE HIGH POWER 7

OBJECTIVE LENSES ARE NEAR THE SPECIMEN

CONDENSOR/IRIS

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IT CONVERGES THE LIGHT ON TO THE SPECIMEN

NON OPTICAL PARTS ARE

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STAGE, HANDLE, ADJUSTMENT KNOBS

SLIDE MOVEMENT KNOBS9

TYPES OF MICROSCOPES• DARK FIELD MICROSCOPE• PHASE CONTRAST

MICROSCOPE• FLOURESENT

MICROSCOPE• ELECTRON MICROSCOPE• A beam of electrons are

used for illumination and it is recorded and viewed, it increases the resolution

• A light object is seen on a dark background, e.g.. Spirochetes, syphilis bacteria can be seen by dark field microscope.

• Live unstained microorganism can be seen by Phase contrast microscope.

• Ultraviolet light is used to illuminate the specimen by fluorescent microscope.

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Types of tissues

• There are four basic tissues of our body.• 1. Epithelial tissue,• 2. Connective tissue,• 3. Muscular tissue,• 4. Nervous tissue.

05/03/23These are fundamental

tissues of the body11

SIMPLE EPITHELIUM1. It is a single layered structure, the cells are arranged side by side.2. No spaces in between.3. No extra cellular material.4. No blood vessels/nerves…

05/03/23FEATURES OF SIMPLE

EPITHELIUM12

TYPES OF SIMPLE EPITHELIUM

• 1. SIMPLE COLUMNAR• 2.SIMPLE SQUAMOUS• 3.SIMPLE CUBOIDAL• 4. PSEUDOSTRATIFIED

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CLASSIFICATION OF SIMPLE EPITHELIUM 13

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CILIA ARE MOTILE STRUCTURES ARISING FROM LUMINAL SURFACE OF EPITHELIUM

SIMPLE COLUMNAR EPITHELIUM1. CELLS ARE ARRANGED IN ONE ROW.2. TALL COLUMNAR CELLS3. ALL RESTING ON BASEMENT MEMBRANE4. HELPS IN ABSORPTION/SECRETION

05/03/23 FEATURES 15

EXAMPLES

1.GALL BLADDER2.STOMACH3.INTESTINE4.UTERINE TUBE

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SIMPLE COLUMNAR EPITHELIUM 16

SIMPLE SQUAMOUS EPITHELIUM

• A single row of thin scale like cells resting on a basement membrane.

• Helps in perfusion/filtration/lining the blood vessels/peritoneum/pleura/pericardium.

• Examples are seen in lung alveoli, • Bowman’s capsule of kidney.

05/03/23 FEATURES AND EXAMPLES 17

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SIMPLE SQUAMOUS EPITHELIUMSEEN IN THE INNER LINING OF THE BLOOD VESSELS

05/03/23 TUNICA INTIMA/ENDOTHELIUM 19

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SIMPLE SQUAMOUS EPITHELIUM

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05/03/23 LUNG ALVEOLI 23

Simple squamous epithelium

05/03/23SIMPLE CUBOIDAL

EPITHELIUM 24

SIMPLE CUBOIDAL EPITHELIUMSEEN IN CONVOLUTED TUBULES OF KIDNEY

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LINING CELLS OF THYROID FOLLICLES

SIMPLE CUBOIDAL EPITHELIUM

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SECRETION/EXCRETION/ABSORPTION FUNCTIONS OF

SIMPLE CUBOIDAL EPITHELIUM26

PSEUDOSTRATIFIED CILIATED COLUMNAR EPITHELIUM

RESPIRATORY EPITHELIUM

05/03/23 TRACHEA 27

FEATURES OF REPIRATORY EPITHELIUM

• MULTILAYERED CELLS• NUCLEI AT VARIOUS LEVELS• SURFACE CELLS HAVING CILIA• SIMPLE GOBLET CELLS • CONTAINING MUCOUS• ALL CELLS REST ON BASEMENT MEMBRANE

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RESPIRATORY EPITHELIUM

CILIA AT THE FREE APICAL SURFACE

05/03/23FALSE APPEARANCE OF

MULTILAYERD CELLS29

IDENTIFY THE TYPE OF EPITHELIUM ?

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What type of epithelium is this?

Where it can be found?

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Identify the type of epithelium?

Where are the places it can be found ?

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WHAT TYPE OF EPITHELIUM IS THIS?

WRITE ANY 2 FEATURES OF THIS TYPE OF EPITHELIUM.

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