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EXPRESSION AND FUNCTION OF MICRORNA IN HUMAN CANCER
DISSERTATION
Presented in Partial Fulfillment of the Requirements for the
Degree Doctor of Philosophy in the Graduate School of
The Ohio State University
By
Eun Joo Lee, M.S.
*****
The Ohio State University
2008
Dissertation Committee:
Dr. Thomas D. Schmittgen, Adviser Approved by
Dr. James T. Dalton
Dr. Terry S. Elton
Dr. Mamuka Kvaratskhelia Adviser
Graduate program in Pharmacy
Copyright by
Eun Joo Lee
2008
ii
ABSTRACT
microRNAs (miRNA) are functional 22 nt noncoding RNAs that negatively
regulate gene expression. The mature miRNA binds to the 3’ untranslated region of the
target messenger RNA (mRNA) and represses translation. There is mounting evidence
that miRNAs are involved in a number of human cancers. Pancreatic cancer is the fourth
leading cause of cancer-related death in the United States. The prognosis for pancreatic
cancer is the worst of all cancers. Better understanding of the genetic alterations in
pancreatic cancer may improve the early detection and treatment regimens.
The expression of over 200 miRNA precursors on specimens of human pancreatic
adenocarcinoma, paired benign tissue, normal pancreas, chronic pancreatitis and
pancreatic cancer cell lines was profiled using a real-time PCR assay developed in our lab.
The results showed that a number of miRNAs were differentially upregulated in the
tumors and cell lines compared to the normal pancreas tissue. The expression of the
active, mature miRNA was validated using a Taqman real-time PCR assay and Northern
blotting. Finally, RT in situ PCR was performed to verify that aberrantly expressed
miRNAs were localized to tumor cells and not to stroma or normal acini or duct cells.
To explore the mechanism of overexpressed miRNAs in pancreatic cancer and to
evaluate potential therapeutics, antisense oligonucleotides (ASO) were used to inhibit the
activity of the overexpressed miRNAs in pancreatic cancer. ASO produced cytotoxicity
iii
to pancreatic cancer cell lines in the low nM range. Cytotoxicity was less pronounced
when the normal ductal epithelial cell line HPNE was exposed to ASO or when
pancreatic cancer cell lines were exposed to an irrelevant control oligonucleotide.
Inhibition of miRNA with antisense increased the amount of apoptosis and resulted in
cell cycle arrest in HS766T cells compared to the control oligonucleotide. Synergistic
cytotoxicity was observed when ASO was combined with gemcitabine. The mRNA
targets of the aberrantly expressed miRNA in pancreatic cancer were validated and
identified to determine mechanism of action.
The miRNA processing pattern in different tissues and cell lines were determined
and a large number of miRNAs are transcribed but are not processed to the mature
miRNA.
iv
DEDICATION
To my parents
v
ACKNOWLEDGMENTS
I would like to express sincere appreciation and gratitude to my advisor, Dr.
Thomas D. Schmittgen for his support and guidance. I have been very fortunate to have a
great teacher in the past five years who stimulated my interest in microRNA research.
Dr.Schmittgen guided my efforts to learn about microRNA and cancer and he always
encouraged me with his enthusiasm. I also would like to thank the members of my
dissertation committee, Dr. James T. Dalton, Dr. Elton S. Terry, and Dr. Mamuka
Kvaratskhelia for their comments and discussions.
I appreciate Yuriy Gusev for statistical analysis of my studies. I would like to
thank Daniel J. Brackett, Megan R. Lerner, Daniel L. Morgan, and Russell G. Postier for
providing human pancreatic tissues and cell lines and I would like to thank Dr. Wendy L.
Frankel for tissue specimen examination. I appreciate Dr. Caifu Chen for support of
Taqman real-time PCR assays and experimental discussions. I am grateful to Dr.
Christine Esau for support of antisense oligonucleotides and expertise on antisense
studies. I also would like to thank to Dr. Gerard J. Nuovo for In situ RT PCR work.
I would like to thank Dr. Jinmai Jiang, Jong-Kook Park and Myungwon Baek for
their assistance with experiments, implementation, and data interpretation of the studies
performed herein. I wish to thank Ana Clara Azevedo and Liuqing Yang for their
friendship and help.
vi
I would like to thank my parents, Sungho Lee and Keumsook Jeong, and my
brothers, Hyoshik Lee and Yongshik Lee, for their love, encouragement, and support.
I would like to thank Proctor and Gamble for the Fellowship. This research was
supported by a grant from NIH (CA107435-01 to T.D.S.) and the National Pancreas
Foundation.
vii
VITA
November 29, 1975…………………... Born in Ulsan, South Korea
1995 – 1999……………………………B.S. Pharmacy, Seoul National University
Seoul, South Korea
1999 - 2001………………………….…M.S. Pharmacy, Seoul National University
Seoul, South Korea
2003 - Present……………………….…Graduate Teaching and Research Associate,
College of Pharmacy, The Ohio State University
PUBLICATIONS
1. Schmittgen, T.D., Lee, E.J., Jiang, J., Sarkar, A., Yang, L., Elton, T.S., Chen, C.
Real-time PCR quantification of precursor and mature microRNA. Methods,
44(1):31-38, 2008.
2. Lee, E.J., Baek, M., Gusev, Y., Brackett, D.J., Nuovo, G.J., Schmittgen, T.D.
Systematic evaluation of microRNA processing patterns in tissues, cell lines, and
tumors. RNA, 14(1):35-42, 2007.
3. Calin, G.A., Liu, C.G., Ferracin, M., Hyslop, T., Spizzo, R., Sevignani, C., Fabbri,
M., Cimmino, A., Lee, E.J., Wojcik, S.E., Shimizu, M., Tili, E., Rossi, S.,
Taccioli, C., Picchiori, F., Liu, X., Zuppo, S., Herlea, V., Gramantieri, L., Lanza,
G., Alder, H., Rassenti, L., Volinia, S., Schmittgen, T.D., Kipps, T.J., Negrini, M.,
Croce, C.M. Ultraconserved regions encoding ncRNAs are altered in human
leukemias and carcinomas. Cancer Cell, 12(3):215-229, 2007.
viii
4. Lee, E.J., Gusev, Y., Jiang, J., Nuovo, G.J., Lerner, M.R., Frankel, W.L., Morgan,
D.L., Postier, R.G., Brackett, D.J., Schmittgen, T.D. Expression profiling
identifies microRNA signature in pancreatic cancer. International Journal of
Cancer, 120(5):1046-1054, 2007.
5. Lee, E.J. and Schmittgen, T.D. Comparison of RNA assay methods used to
normalize cDNA for quantitative real-time PCR. Analytical Biochemistry,
357(2):299-301, 2006.
6. Martin, M.M., Lee, E.J., Jiang, J., Buckenberger, J., Schmittgen, T.D., and Elton,
T.S. microRNA-155 regulates human angiotensin II type 1 receptor expression in
fibroblasts. Journal of Biological Chemistry, 281(27):18277-18284, 2006.
7. Jiang, J., Lee, E.J. and Schmittgen, T.D. Increased expression of microRNA-155
in Epstein-Barr Virus transformed lymphoblastoid cell lines. Genes,
Chromosomes & Cancer, 45(1):103-106, 2006.
8. Jiang, J., Lee, E.J., Gusev, Y and Schmittgen, T.D. Real-time expression
profiling of microRNA precursors in human cancer cell lines. Nucleic Acids
Research, 33(17):5394-5403, 2005.
9. Lee, E.J., Kim, E.J., Kim, Y.G., Chung, H.C., Kim, S.H., Kim, D.H., Lee, I., Kim,
S.G., Lee, M.G. Pharmacokinetic changes of intravenous 2-(allylthio) pyrazine, a
chemoprotective agent, in rats with acute renal failure induced by uranyl nitrate.
Research Communications in Molecular Pathology and Pharmacology, 111(5-
6):225-236, 2002.
10. Shim, H.J., Lee, E.J., Jung, Y.H., Kim, S.H., Kim, S.H., Yoo, M., Kwon, J.W.,
Kim, W.B., Lee, M.G. Determination of a new phosphodiesterase V inhibitor,
DA-8159, in plasma and urine by high-performance liquid chromatography.
Journal of Pharmaceutical and Biomedical Analysis, 30(3):527-533, 2002.
11. Shim, H.J., Lee, E.J., Kim, J.H., Kim, S.H., Kwon, J.W., Kim, W.B., Cha, S.W.,
Lee, M.G. Subacute toxicities and toxicokinetics of a new erectogenic, DA-8159,
after single and 4-week repeated oral administration in dogs. Biopharmaceutics &
Drug Disposition, 22(3):109-117, 2001.
12. Shim, H.J., Lee, E.J., Kim, S.H., Kim, S.H., Yoo, M., Kwon, J.W., Kim, W.B.,
Lee, M.G. Factors influencing the protein binding of a new phosphodiesterase V
ix
inhibitor, DA-8159, using an equilibrium dialysis technique. Biopharmaceutics &
Drug Disposition, 21(7):285-291, 2000.
13. Shim, H.J., Lee, E.J., Kim, S.H., Kim, S.H., Yoo, M., Kwon, J.W., Kim, W.B.,
Lee, H.S., Lee, M.G. Pharmacokinetics, stability, and blood partition of DA-8159,
a new phosphodiesterase V inhibitor. Research Communications in Molecular
Pathology and Pharmacology, 108(3-4):275-286, 2000.
14. Hurh, E., Lee, E., Lee, A., Kim, Y., Kim, S., Kim, S., Lee, M. Effects of enzyme
inducers or inhibitors on the pharmacokinetics of intravenous parathion in rats.
Biopharmaceutics & Drug Disposition, 21(5):193-204, 2000.
15. Hurh, E, Lee, E.J., Kim, Y.G., Kim, S.Y., Kim, S.H., Kim, Y.C., Lee, M.G.
Effects of physostigmine on the pharmacokinetics of intravenous parathion in rats.
Biopharmaceutics & Drug Disposition, 21(8):331-338, 2000.
16. Hurh E, Lee, E.J., Kim, Y.G., Kim, S.Y., Kim, S.H., Kim, Y.C., Lee, M.G.
Effects of neostigmine on the pharmacokinetics of intravenous parathion in rats.
Research Communications in Molecular Pathology and Pharmacology, 108(3-
4):261-273, 2000.
FIELDS OF STUDY
Major Field: Pharmacy
x
TABLE OF CONTENTS
ABSTRACT ......................................................................................................................ii
DEDICATION .................................................................................................................iv
ACKNOWLEDGMENTS ................................................................................................v
VITA ................................................................................................................................vii
LIST OF TABLES .........................................................................................................xvi
LIST OF FIGURES ......................................................................................................xvii
CHAPTERS
1. INTRODUCTION ................................................................................................1
1.1 miRNA DISCOVERY ......................................................................................1
1.2 miRNA BIOGENESIS .....................................................................................3
1.3. miRNA TARGET IDENTIFICATION ...........................................................6
1.4 miRNA EXPRESSION AND PROFILING .....................................................8
1.5 miRNA EXPRESSION IN HUMAN CANCER ............................................10
1.6 miRNA THERAPEUTICS .............................................................................14
1.6.1 Inhibition of miRNA using antisense oligonucleotides (ASO) .......15
1.6.2 Replacement of miRNA ...................................................................16
1.7 OBJECTIVE OF DISSERTATION ...............................................................17
xi
2. EXPRESSION PROFILING IDENTIFIES MICRORNA SIGNATURE IN
PANCREATIC CANCER ..................................................................................21
2.1 INTRODUCTION ..........................................................................................21
2.2 MATERIAL AND METHODS ......................................................................22
2.2.1 Tissue procurement ..........................................................................22
2.2.2 Cell lines ..........................................................................................23
2.2.3 miRNA precursor expression profiling ............................................23
2.2.4 Real-time PCR of mature miRNA ...................................................24
2.2.5 Northern blotting ..............................................................................25
2.2.6 RT in situ PCR .................................................................................25
2.2.7 Statistics ...........................................................................................26
2.3 RESULTS .......................................................................................................28
2.3.1 Validation .........................................................................................28
2.3.2 miRNA precursor profiling in pancreatic tissues .............................28
2.3.3 Data analysis by prediction analysis of micro-array algorithm (PAM)
..........................................................................................................30
2.3.4 Validation of miRNA expression .....................................................31
2.4 DISCUSSION .................................................................................................33
2.5 ACKNOWLEDGEMENTS ............................................................................37
xii
3. ANTISENSE INHIBITION OF microRNA REDUCES CELL VIABILITY
AND SENSITIZES THE EFFECT OF GEMCITABINE IN PANCREATIC
CANCER .............................................................................................................48
3.1 INTRODUCTION ..........................................................................................48
3.2 MATERIAL AND METHODS ......................................................................52
3.2.1 Cell lines and culture .......................................................................52
3.2.2 miRNA profiling ..............................................................................52
3.2.3 Antisense oligonucleotides ..............................................................53
3.2.4 Antisense oligonucleotides transfection ..........................................53
3.2.5 Gemcitabine treatment .....................................................................53
3.2.6 Cell proliferation assay ....................................................................54
3.2.7 Combination assay ...........................................................................54
3.2.8 Data analysis ....................................................................................55
3.2.9 Statistical analysis ............................................................................56
3.3 RESULTS .......................................................................................................56
3.3.1 miRNA expression in pancreatic cell lines ......................................56
3.3.2 Antiproliferative effects of antisense oligonucleotides ....................57
3.3.3 Schedule-dependent combinatorial effects of antisense with
gemcitabine ......................................................................................59
3.3.4 Evaluation of synergism in ASO combination ................................60
xiii
3.4 DISCUSSION .................................................................................................62
3.5 ACKNOWLEDGEMENTS ............................................................................63
4. ANTISENSE INHIBITION OF microRNA ARRESTS CELL CYCLE,
INDUCES APOPTOSIS AND IDENTIFIES THE TARGET MOLECULES
IN PANCREATIC CANCER ............................................................................77
4.1 INTRODUCTION ..........................................................................................77
4.2 MATERIAL AND METHODS ......................................................................80
4.2.1 Cell lines and culture .......................................................................80
4.2.2 Antisense Oligonucleotides and Pre-miR™ miRNA Precursor ......80
4.2.3 Antisense oligonucleotide and Pre-miR™ miRNA Precursor
transfection ......................................................................................80
4.2.4 Cell cycle analysis ............................................................................80
4.2.5 Apoptosis analysis ...........................................................................81
4.2.6 Real-time PCR .................................................................................81
4.2.7 Plasmid constructs ...........................................................................82
4.2.8 Luciferase assay ...............................................................................82
4.2.9 Western blotting ...............................................................................83
4.3. RESULTS ......................................................................................................83
4.3.1 Effects of miRNA inhibition on the cell cycle .................................83
4.3.2 Effect of miRNA inhibition on apoptosis ........................................84
xiv
4.3.3 PTEN, RECK and p27(kip1) validation as target of miR-21 and miR-
221 ....................................................................................................85
4.3.4 FOXO3a as a new target of miR-212 ..............................................86
4.4 DISCUSSION .................................................................................................88
4.5 ACKNOWLEDGEMENTS ............................................................................91
5. SYSTEMIC EVALUATION OF microRNA PROCESSING PATTERNS IN
TISSUES, CELL LINES AND TUMORS ........................................................97
5.1 INTRODUCTION ..........................................................................................97
5.2 MATERIAL AND METHODS ......................................................................99
5.2.1 Cells, tissues and RNA ....................................................................99
5.2.2 Real-time RT PCR, miRNA precursors, and mature miRNA .........99
5.2.3 Real-time RT PCR, absolute copy number for precursor and mature
miRNA .........................................................................................100
5.2.4 Northern blotting ............................................................................101
5.2.5 In situ detection of miRNA ............................................................101
5.2.6 Data analysis ..................................................................................101
5.3 RESULTS .....................................................................................................102
5.4 DISCUSSION ...............................................................................................107
5.5 ACKNOWLEDGMENTS ............................................................................109
6. SUMMARY AND DISCUSSION ....................................................................117
xv
BIBLIOGRAPHY..........................................................................................................124
APPENDICES
APPENDIX A: Data Relevant to Chapter 2 .......................................................147
Table A ....................................................................................................148
Figure A ..................................................................................................198
APPENDIX B: Data Relevant to Chapter 3 .......................................................199
Table B ....................................................................................................200
APPENDIX C: Data Relevant to Chapter 5 .......................................................215
Table C.1..................................................................................................216
Table C.2..................................................................................................251
Table C.3..................................................................................................287
Table C.4..................................................................................................302
Table C.5..................................................................................................316
Table C.6..................................................................................................321
xvi
LIST OF TABLES
Table Page
2.1 Clinical data and tumor pathology ........................................................................46
2.2 Top 20 aberrantly expressed miRNA precursors in pancreatic adenocarcinoma .47
3.1 Sequence and chemical modifications to antisense oligonucleotides ...................73
3.2 miRNA expression, pancreas cancer cell lines normalized to normal pancreas cell
(HPNE) .................................................................................................................74
3.3 Antiproliferative activity parameters of ASO against miR-21, -100, -212, -221
and -301 in human pancreatic cell lines ................................................................76
5.1 microRNA that display tissue-specific or tissue enriched expression ................115
5.2 Relative expression levels of mature miRNA and miRNA� forms ....................116
xvii
LIST OF FIGURES
Figure Page
1.1 miRNA biogenesis ................................................................................................19
1.2 Chemical modification of antisense oligonucleotides ..........................................20
2.1 18S rRNA expression in pancreatic tissue ............................................................38
2.2 miRNA precursor expression in pancreatic samples ............................................39
2.3 Three dimensional expression terrain map ...........................................................40
2.4 Estimated probabilities for the training and test data ............................................41
2.5 miRNA expression by Northern blotting ..............................................................42
2.6 Validation of precursor and mature miRNA levels ..............................................44
2.7 Histologic and molecular analysis of pancreatic cancer for microRNA
expression .............................................................................................................45
3.1 Structures of gemcitabine and deoxycytidine .......................................................65
3.2 Comparison of three antisense oligonucleotides ...................................................66
3.3 The effect of antisense oligonucleotide and control oligonucleotide in pancreatic
cell lines ................................................................................................................68
3.4 Effect of control oligonucleotides on cell proliferation ........................................69
3.5 ASO pretreatment sensitized the antiproliferative effects of gemcitabine ...........71
3.6 Combination index (CIx) versus fraction affected (fa) plots in HS766T cells .....72
xviii
4.1 Flow cytometric analysis of the effect of antisense oligonucleotide (ASO) on cell
cycle of HS766T cells ...........................................................................................92
4.2 Flow cytometric analysis of apoptosis in HS766T cells .......................................93
4.3 Western blot analysis of PTEN, RECK and p27(kip1) in HS766T cells treated
with antisense to miR-21 or miR-221 ...................................................................94
4.4 Alignment of the FOXO3a binding site with miR-212 and mRNA expression of
FOXO3a after ASO treatment .............................................................................95
4.5 Target validation by luciferase assay and FOXO3a western blotting ...................96
5.1 microRNAs with good correlation in expression levels of precursor and
mature .................................................................................................................110
5.2 Expression of miR-128a in 22 normal tissues and cells .....................................111
5.3 Lack of microRNA processing in cancer cell lines ............................................112
5.4 miR-31 expression in cancer cell lines ...............................................................113
5.5 Pearson correlation among the various samples studied .....................................114
6.1 Pancreatic cancer progression model and genetic alteration .............................123
1
CHAPTER 1
INTRODUCTION
1.1 miRNA DISCOVERY
Until very recently, the primary role of RNA was believed to carry genetic
information for protein translation. The discovery of small non-coding RNAs and other
noncoding RNAs has caused many to rethink the role of RNA in biology. microRNA
(miRNA) is endogenous ~ 22 nt non-coding RNA that negatively regulates gene
expression (1-3). miRNA was discovered by the Ambros and Ruvkun laboratories during
genetic screening of developmental progress in C. elegans (4, 5). The lin-4 gene
produced ~ 22 nt noncoding RNA that binds to sequences in the 3’ untranslated region
(3’ UTR) of lin-14 mRNA (4). The interaction between lin-4 and lin-14 caused
translational repression of the lin-14 mRNA (4). The Ruvkun laboratory also discovered
that another small non-coding RNA, let-7, recognized the 3’ UTR of lin-41 mRNA and
repressed LIN-41 protein levels (6). let-7 was conserved in a wide range of animals,
suggesting that other ~ 22 nt regulatory RNAs may exist in other organisms (7).
Shortly thereafter, RNA interference (RNAi) was uncovered in C. elegans by the
Fire and Mello laboratory. They discovered that double-stranded RNA (dsRNA)
2
produced target gene silencing, in which the target mRNA is cleaved in a sequence-
specific manner (8). Twenty-two to twenty-five nt duplex RNA, named small interfering
RNAs (siRNAs), was discovered by the Tuschl laboratory (9). siRNAs mediate
sequence-specific mRNA degradation during RNA interefence (RNAi). siRNA is
generated from long dsRNA by the RNase III, Dicer and it is incorporated into the RNA
induced silencing complex (RISC). mRNA that is 100% complementary to the siRNA
(10) is cleaved. RNAi has been a powerful tool of gene silencing and has been applied in
a wide range of human diseases.
In 2001, interest in the field peaked when hundreds of miRNAs were discovered
in Drosophila, C. elegans and mammals (1-3). These ~ 22 nt endogenous RNAs, derived
from longer hairpin precursors, were named microRNAs (miRNAs). Using
bioinformatics and cDNA cloning methods, miRNA sequences were uncovered based
upon the following criteria; expression of ~ 22 nt mature RNA in one arm of the hairpin
structure, location in intergenic regions, DNA sequence homology between similar
species and containing a stem-loop structure in the precursor transcript (1-3). The
expression of miRNAs was also examined during larval stages and young adults in C.
elegans and gene expression was varied in different stages of development,
demonstrating that they play an important role in developmental timing (1-3). A number
of miRNAs have been identified in a wide range of species such as plant, nematodes,
fruit flies, and human and more miRNA genes are expected to be discovered in many
species (11). At the time of writing, 678 Homo sapiens miRNAs have been discovered
(12).
3
1.2 miRNA BIOGENESIS
Human miRNAs are distributed in all chromosomes except the Y chromosome.
Initially, miRNAs were thought to be located only within intergenic regions (1-3).
However, Rodriguez et al. reported that around 70% of mammalian miRNAs are located
in defined transcription units (TU) i.e. intronic and exonic miRNAs (13). Among those
miRNAs, 61% RNAs are located in the introns of protein-coding TU, 18% are located in
the introns of non-coding TU and some are found in exons of non-coding gene (20%)
(13). Most miRNAs are transcribed by RNA polymerase II. However, miRNAs
positioned within Alu-repetitive elements are transcribed by RNA polymerase III (14).
Some miRNAs are clustered and transcribed together as long polycistronic RNAs.
Clustered miRNAs seem to be functionally linked to each other (15). miR-23a, -27a, -
24-2 and miR-17, -18, -19a, -20, -19b-1 are examples of polycistronic miRNA transcripts
(16). An overall picture of miRNA biogenesis is shown in Figure 1.1.
The primary transcript, called the pri-miRNA contains a 5’ 7-methyl guanosine
cap and 3’ polyadenylate tail (17, 18). Pri-miRNA is processed in the nucleus by Drosha,
an RNase III endonuclease to produce 60 to 70 nt pre-miRNA; the flanking sequences
around the hairpin structure are believed to be degraded (19). The ~ 160 kDa Drosha
crops the stem of the pri-miRNA hairpin structure asymmetrically, i.e. it produces a 2 nt
3’ overhang on the pre-miRNAs. Drosha forms a microprocessor complex of ~ 650 kDa
with the DiGeorge syndrome critical region gene 8 (DGCR8); this complex is conserved
from flies to human (20-23). Drosha shows non-specific RNase activity, but DGCR8
contains two double-stranded RNA binding sites at the C-terminus. This helps Drosha
4
correctly recognize and cleave the hairpin structure at the specific site (20-23). DGCR8
also has a WW domain at the N-terminus, which is believed to interact with proline-rich
peptides and in the proline-rich N-terminus of Drosha (20). Neither Drosha nor DGCR8
shows any processing activity independently (22).
The pre-miRNAs are then exported to the cytoplasm by Exportin-5; a Ran-
dependent nuclear transport receptor (24). Exportin-5 especially transports the minihelix
motif RNAs, which contain a double-stranded stem (> 14 nt) with 3' overhang on the end
(25). ILF3, which is a nuclear protein containing two double-stranded RNA binding
domain and RGG domain forms a quaternary complex with the minihelix motif RNA and
Exportin-5, indicating that ILF3 facilitates transport of pre-miRNA along with Exportin-5
(26). It was believed that if Exportin-5 is inhibited by siRNA, the pre-miRNA would
accumulate and mature miRNA would decrease (24). However, pre-miRNA did not
accumulate in the nucleus, which suggests that pre-miRNA is a quite unstable
intermediate in the absence of Exportin-5 (24).
In the cytoplasm, pre-miRNAs are processed into ~ 22 nt mature miRNA by
another RNase III endonuclease, Dicer (27). Dicer is a highly conserved protein. Dicer
binds with other cofactors such as Loquacious/HIV-1 TAR RNA binding protein (TRBP)
(28, 29). Dicer cleaves the loop of the hairpin to produce the miRNA:miRNA� duplex.
The duplex contains a 5’ phosphate and 2 nt 3’ overhang. Dicer-TRBP recruits
Argonaute protein (Ago 2) to form the RNA-induced silencing complex (RISC)
incorporating the mature miRNAs into the RISC (30). One strand of the duplex miRNA
is degraded and the other strand remains as the mature miRNA. The decision by RISC
as to which strand to degrade is based on base pair stability at the 5’ ends of the duplex
5
miRNA; the miRNA strand with relatively lower stability at 5’ end will remain in RISC
(31).
Finally, the mature miRNA that is incorporated into RISC binds to the 3’ UTR of
the target mRNA and mediates translational repression or target cleavage according to
sequence complementary between miRNA and target mRNA (31). If the mRNA and
miRNA have perfect complementary, miRNA will cleave the mRNA much like a siRNA.
In animals, it is more likely that the miRNA/mRNA interaction will not have perfect
complementarity and the miRNA will repress translation (32). mRNA destabilization is
caused by deadenylation and decapping (33). Translational initiation or elongation might
also be inhibited (34, 35). However, the Steitz laboratory recently reported that miRNA
may also up-regulate translation during cell cycle arrest, indicating that translational
regulation by miRNA can be either repressive or activating (36). miRNA is generally
thought to bind to the sequence of 3’ UTR. However, recent reports showed that miRNA
can also bind to other sites. For example, let-7 binds to the mRNA target sites in either
the coding region or the 5’ UTR in zebrafish (37) and the Steitz laboratory demonstrated
that miRNA binds the target mRNA efficiently in the 5’ UTR as in the 3’ UTR (38).
Also, the Lund laboratory showed that miR-10a binds to the 5’ UTR of ribosomal
proteins mRNA and enhances the translation, suggesting that miR-10a may control global
protein synthesis (39). These studies imply that miRNA has more diverse and complex
functions.
Recent work has demonstrated that miRNA biogenesis may be regulated in
developmental stages or specific tissues (40-44). One control mechanism is
transcriptional regulation. let-7 is regulated by a transcriptional enhancer element in C.
6
elegans (41). Another mechanism is post-transcriptional regulation. pri-miR-18a
requires an RNA-binding protein hnRNP A1 for processing to the precursor (40). The
RNA binding protein, lin-28 inhibits the processing of pri-let-7 miRNAs in embryonic
cells (44). pre-miR-138-2 is restricted to Dicer cleavage in certain tissues and cell types
(43). Finally RNA editing, which converts adenosine (A) to inosine (I), can inhibit Dicer
cleavage. Edited pre-miRNA accumulates, indicating that Dicer-TRBP complex cannot
bind to edited pre-miRNA (42).
1.3 miRNA TARGET IDENTIFICATION
miRNAs control gene expression by binding to the 3’ UTR of target mRNA. It is
estimated that approximately 30% of human genes are regulated by miRNA (45).
Although 678 human miRNAs have been reported, identification and validation of their
targets has been a challenge. Knowing the miRNA’s target is crucial to identifying the
miRNAs function.
The first miRNA target was the lin-14 gene (4). Further studies (46, 47)
identified the criteria for the miRNA/mRNA interaction by mutating known target
binding sites. Of particular importance is the 5’ seed region of the miRNA. This 5’ seed,
consisting of nts 2-8, must bind perfectly to the target mRNA sequence. The Cohen
laboratory reported the minimal target site for miRNA function (46). They mentioned
that seven or more sequences that are complementary at the 5’ end of the miRNA are
enough to show function. If the 5’ sequence has weaker binding, then the 3’
compensatory binding is required. However, extensive binding at the 3’ site without 5’
7
complementarity is not enough to have miRNA function (46). Even though 5’
complementary sequence is important to predict the target gene, 3’ complementary site is
also important to distinguish the target of miRNA isoforms. miRNA isoforms may target
the same gene or different genes. For example, let-7 family members (e.g. let-7, miR-48,
miR-84 and miR-241) have weak seed binding to one known target (lin-41), but only let-
7 has strong 3’ binding and lin-41 is regulated by only let-7 among let-7 family members.
In addition, G:U wobble in the seed sequence also showed negative effect in binding
activity (46).
Other elements may affect target binding. Secondary structure in 3’ UTR of the
mRNA may prevent the miRNA from binding to the site (48). Several laboratories imply
that target regulation by miRNA is also dependent on 3’ UTR context. For example, the
function of CAT-1, which is a target of miR-122, depends on the presence of HuR
protein binding site (49) and lsy-6 functionally binds to the target site (cog-1) in
endogenous 3’ UTR, but not in a heterologous 3’ UTR (50).
Several target prediction programs have been developed. The main algorithms
used in these programs include a 5’ seed sequence match and a calculation for the
thermodynamic stability between miRNA and mRNA. TargetScan
(http://www.targetscan.org) (45, 47, 51) is one program used to predict mammalian
miRNA targets. It finds conserved 8 mer and 7 mer sites that match the seed sequence of
the miRNA and calculates the free energy using the RNA Fold algorithm. A false
positive rate of between 22-31% may be expected from TargetScan. PicTar
(http://pictar.bio.nyu.edu) (52-54) is used to identify miRNA target genes in C. elegans,
Drosophila, and vertebrates. It identifies targets for single miRNA as well as targets that
8
are regulated by several miRNAs in a coordinated manner. It predicts around 200 targets
per miRNA with an estimated false positive rate of around 30%. Lastly, miRanda
(http://microrna.sanger.ac.uk) (55) uses sequence match and binding energy between the
miRNA and the predicted target site. Evolutionary conservation of the target binding site
is applied; miRanda has a 24-39% false positive rate.
However, prediction programs don’t account for all of the criteria. For example,
3’ UTR accessibility and/or 3’ UTR context (specific RNA- or protein-based cofactors)
as well as the 5’ seed region and thermodynamics may be important criteria for target
prediction. Combining all these possible factors can produce better performance to
predict the miRNA target. Moreover, a single miRNA targets numerous genes and one
target gene is regulated by multiple miRNAs. This suggests that miRNA and target
network might be more complicated than originally expected and that experimental
validation should be performed carefully.
1.4 miRNA EXPRESSION AND PROFILING
miRNA profiling is a major discovery tool to identify tissue-specific or disease-
specific miRNAs. Several methods are available to profile miRNA expression. These
include Northern blotting, microarray, real-time PCR and in situ hybridization. The
benefit of Northern blotting is that both mature and precursor transcripts are visualized at
the same time on the blot. However, it is a laborious technique with poor sensitivity and
requires large amounts of RNA (10-30 µg). Another method is cDNA microarray (56).
cDNA arrays are used in many laboratories for high-throughput screening since
9
thousands of genes may be placed on the array. cDNA microarray is based on the
hybridization between the target gene and probe. However, it often shows low sensitivity
and variation in signal intensity (57). In addition, microarray data should be corrected
from background and normalized to reduce dye labeling bias, variations in hybridization
and scanning (58). Therefore, the microarray data should be confirmed by northern blot
or real-time PCR. Real-time PCR is the most sensitive of the high-throughput screening
methods. Performing real-time PCR on miRNA is a challenge because the precursors
exist as a stable hairpin and the mature miRNA is ~ 22 nt, roughly the size of a standard
PCR primer. The Schmittgen laboratory developed a PCR assay to amplify precursor
miRNA as a means to predict the mature miRNA expression (59). Shortly thereafter,
Applied Biosystems developed a real-time PCR assay to the the mature miRNA (60).
This assay is named the looped primer PCR assay because the RT primer consists of a
stable loop structure. Stem-loop RT primers were developed for several reasons. It
increases the annealing temperature to the mature miRNA. It also avoids precursor
miRNA or genomic DNA from being amplified. Finally, it extends the size of the ~ 22 nt
mature miRNA. Since the mature assay uses TaqMan probes, it enhances sensitivity to
distinguish miRNA family members (61). The assay is sensitive enough to profile the
miRNA expression from a single cell (62, 63).
miRNA profiling of human cancers is a useful tool to classify cancer type
according to their origin. Jiang et al. (61) profiled around 200 miRNA precursors in 32
human cancer cell lines and hierarchical clustering showed that most of cell lines are
clustered into each tissue type based on their origin. Also, human pancreatic tumor
tissues and normal tissues were used to profile miRNA precursors and the heatmap
10
results demonstrated that the tumor tissue group was completely separated from the
normal tissue group (64). Golub’s group (65) profiled miRNAs in 334 human samples
using a bead-based flow cytometric method. Many miRNAs were down-regulated in the
tumor samples. Hierarchical clustering classified the epithelial and hematopoietic
samples based on the developmental origins of the tissues. Interestingly, cancers within a
single cell lineage were further discriminated according to their genetic abnormality.
Profiling data from 200 miRNAs correctly classify each tumor group, but profiling data
from around 16,000 mRNAs did not (65). This evidence suggests that miRNA profiling
can distinguish the phenotypic signature of diverse types of cancers and that miRNA may
be used as a potential diagnostic marker.
1.5 miRNA EXPRESSION IN HUMAN CANCER
Several miRNAs are tissue-specific or developmental specific, indicating that
they could play important roles in many biological processes. For example, miRNA-143
regulates adipocyte differentiation (66), miRNA-375 regulates insulin secretion (67),
human embryonic stem cells express a unique set of miRNAs (68), and miRNA also
controls oocyte maturation in Drosophila (69).
miRNA expression also plays a role in the initiation and progression of certain
diseases. miR-1 was identified to play a key role in cardiomyocyte differentiation and
cardiovascular development (70). Human angiotensin II type 1 receptor, which function
as vasoconstrictor and plays a role in the pathogenesis of cardiovascular diseases, is
regulated by miR-155 (71). miRNA is also linked to several neurodegenerative diseases
11
such as X mental retardation syndrome (72), Alzheimer’s disease (73) and Parkinson’s
disease (74).
In addition, there is mounting evidence that miRNAs are involved in a number of
human cancers and that miRNAs may act as both tumor suppressors and oncogenes. A
decrease in the expression of a tumor suppressive miRNA will cause an increase in the
target oncogene. In contrast, an increase in expression of oncogenic miRNAs will lead to
the reduction of the target tumor suppressor. Some miRNAs are overexpressed in cancer
and some are down-regulated in cancer. For example, miR-15, -16 (75), miR-143, -145
(76-78), let-7 (79), miR-199a, -199a� (80-82) and 122a (81, 83) are reduced in cancer.
miR-21 (84-87), miR-221, -222 (88-90), miR-100 (88) and miR-155 (91) are increased in
cancer. It is still uncertain if differentiated miRNA expression is a cause or outcome of
tumor transformation. Kumar et al. (92) knocked down Drosha and Dicer enzymes in
cell lines producing a global reduction of mature miRNA expression. This reduction in
mature miRNA levels caused an increase in cell growth. Using a Dicer deficient lung
cancer mouse model, tumor burden (area, size and number) was increased compared to
control. Furthermore, RAS and c-Myc were up-regulated, suggesting that a global loss of
miRNA boosts tumorigenesis.
Each cancer type shows a specific miRNA expression. Perhaps the best
characterized miRNAs in human cancer is miR-15 and miR-16 in human chronic
lymphocytic leukemia (CLL). Deletion of the chromosome 13q14 region is common in
more than 50% of CLL; miR-15 and miR-16 are located at 13q14.3 (75). The Croce
laboratory showed that miR-15 and miR-16 were deleted or down-regulated in more than
60% of CLL samples and that these miRNAs negatively regulate BCL-2 (93). Another
12
example is the miR-17-92 cluster. This cluster is increased in human B-cell lymphomas
and is located at 13q31.3, which is amplified in B-cell lymphoma (94). He et al. also
showed that expressing the miR-17-92 clutster in mice accelerated c-Myc induced tumor
formation, demonstrating that miR-17-92 cluster is oncogenic. In contrast, c-Myc
negatively regulates the transcription of the miR-17-92 cluster and two miRNAs from
this cluster (miR-17-5p and miR-20a) negatively regulate the transcription factor E2F1
(95), suggesting this cluster functions as a tumor suppressor miRNA. Theses results
suggest that the identical miRNA may show both functions depending on tissue of cell
types. miR-155/BIC RNA is elevated in B-cell lymphomas (91). The expression of miR-
155 was higher in cells with an activated B cell phenotype compared to the germinal
center phenotype. Since patients with activated B cell type lymphoma show worse
prognoses, this suggests that miR-155 may be used for as a diagnostic marker (91). The
Croce laboratory showed that a miR-155 overexpressing transgenic mouse displayed pre-
leukemic pre-B cell proliferation in spleen and bone marrow, followed by B cell
malignancy (96).
The expression of mature miR-143 and miR-145 were decreased in colorectal
neoplasia (76). Interestingly, the expression of the hairpin precursors in normal and
tumor tissues remained constant, demonstrating posttranscriptional processing is reduced
in cancer. In lung cancer, let-7 is down-regulated in the tumor and patients with reduced
let-7 had a reduced postoperative survival (79). RAS oncogene is a possible target gene
of let-7 (97) and Yanaihara et al. reported that high expression of miR-155 and low
expression of let-7a-2 correlated with poor survival in lung cancer (98). Several miRNAs
13
are also involved in human breast cancer (99), papillary thyroid carcinoma (90), primary
glioblastoma (100), and hepatocellular carcinoma (82).
Some miRNAs are implicated in several types of cancer. In particular, miR-21 is
overexpressed in B cell lymphoma (101), CLL (102) and many solid tumors, e.g.
pancreatic cancer (64), ovarian cancer (103), esophageal cancer (104), uterine
leiomyomas (105), head and neck cancer (106), colorectal cancer (107) gastric cancer
(108). It was shown that miR-21 is up-regulated in glioblastoma and knockdown of miR-
21 enhanced apoptosis, suggesting miR-21 targets apoptosis-related genes (87). Iorio et
al. (99) and Si et al. (85) also found that miR-21 is overexpressed in breast cancer. Si et
al. showed that anti-miR-21 oligonucleotide decreased cell growth in vitro and in vivo
and they suggested that miR-21 is an oncogene that is associated with the anti-apoptotic
gene BCL-2. Zhu et al. (109) used two-dimensional gel electrophoresis to identify
targets of miR-21 and found that the tumor suppressor gene tropomyosin 1 (TPM1) as a
target of miR-21 in a breast cancer cell line. The Patel laboratory demonstrated that miR-
21 is highly expressed in hepatocellular cancer (86, 110). Inhibition of miR-21 sensitized
the effect of gemcitabine and miR-21 functioned in cell proliferation, migration and
invasion targeting the phosphatase and tensin homolog of PI3 kinase signaling (PTEN).
Recently, a paper showed that the tumor suppressor gene, programmed cell death 4
(PDCD4), is also negatively regulated by miR-21 and that oncogenic miR-21 promotes
cell transformation (111). Löffler et al. explained that miR-21 contributes to signal
transducer and activator of transcription 3 (Stat3)-mediated survival of myeloma cells
(112).
14
This body of evidence indicates that miRNA may act as oncogenes or tumor
suppressor genes and that miRNAs are involved in cell proliferation, apoptosis, cell cycle,
development and metabolism. Identification of miRNAs and the genes they regulate may
provide potential targets for diagnosis and therapy in human cancer.
An important question is what are the causes of differential expression of miRNA
in cancer? Because cancer is a complex genetic disease, the cause might be complicated.
Many miRNAs are located in genomic regions that are involved in cancer including
minimal regions of loss of heterozygosity (LOH), minimal regions of amplification, or
common breakpoint regions (113). Zhang et al. showed that alterations in the DNA copy
of genes that are involved in miRNA biogenesis, such as Dicer 1 and Argonaute 2 may
regulate miRNA expression in tumors (114). In addition, epigenetic regulation such as
DNA hypomethylation, CpG island hypermethylation and the alterations of histone
acetylation (115), transcriptional, posttranscriptional, and translational regulation may
explain the deregulation of miRNA expression in tumors. For example, miR-124a and
miR-127 are inactivated by hypermethylation in several human cancers (116, 117).
1.6 miRNA THERAPEUTICS
As mentioned above, one third of the human genome is regulated by miRNA.
Since one miRNA targets multiple mRNAs, one deregulated miRNA in cancer can affect
the expression of scores of downstream genes. The expression of miRNA is deregulated
in many types of cancer, indicating that miRNAs play important roles in cancer.
Therefore, cancer-related miRNAs are promising targets for therapeutics.
15
The most common method of inhibiting miRNA is with antisense
oligonucleotides (ASO). Other possible options for miRNA blocking are using target
protector or targeting miRNA processing molecules. Target protector binds to the
miRNA binding site in the 3’ UTR of mRNA and it inhibits the miRNA’s access to the
binding site (118). Target protector has the complementary sequence of the seed region
and additional flanking sequence of the 5’ or 3’ binding site. Many molecules, including
Drosha and Dicer are involved in miRNA processing and inhibiting the processing steps
in cancer with increased miRNA expression could reduce mature miRNA. However, this
method can cause diverse blocking effects on other miRNAs and this technique showed
mild anti tumor effect in vitro experiments (19), suggesting that this is not a practical
approach to inhibit miRNA expression. For replacement of miRNA in cancer with
reduced miRNA expression, miRNA mimetics and miRNA expressing vectors could be
used. Although miRNA-based therapeutics is promising, their effective delivery to
specific tissue is a critical issue that needs to be addressed.
1.6.1 Inhibition of miRNA using antisense oligonucleotides (ASO)
For the past 20 years, the antisense field has progressed to better understand the
mechanism of action, delivery, pharmacokinetics, chemical modifications and function of
antisense (119). Antisense is 15-20 nts that binds to target RNA through Watson-Crick
base-pairing. There are several possible mechanisms of action for antisense. Antisense
may bind to the target RNA inhibiting translation (120, 121) or modulating pre-mRNA
splicing (122). Sense-antisense (RNA-DNA) duplex recruits an endogenous nuclease,
RNase H, which degrades only the target RNA (119). Chemically modified
16
antisense with 2’ sugar modifications and phosphorothioate backbone modification
showed effective inhibition of miRNAs (123-126) (Figure 2). For 2’ sugar modifications,
2’-O-methyl (2’-OMe), 2’-O-methoxyethyl (2’-MOE), 2’-flouro (2’-F) and locked
nucleic acid (LNA) are used in vitro. The modified antisense shows improved activity,
stability and delivery. Other modifications to antisense also enhance the silencing effect.
For example, antisense to miR-122 was linked with cholesterol at the 3’ end. The so-
called Antagomirs produced sustained knock down of miR-122 in mouse liver up to 23
days after injection (127). Inhibition of miR-21 in glioblastoma cells and inhibition of
miR-125b-1 in HeLa cells and PC3 cells results in apoptosis and decreased cell viability
(87, 128). These data suggest that antisense may be used to inhibit miRNA and that it
can be used as a potential therapy in cancer treatment.
However, antisense may have “off-target” effects. Antisense and random target
genes may bind to each other producing an unexpected non-target effect. One paper
demonstrated that phosphorothioate backbone modification showed non-antisense effects
through binding of cellular proteins (129). To measure the off-target effects of antisense,
several mismatch control oligos containing the same nucleotide composition as the
antisense should be tested and selection of good control oligonucleotides, better
modification and alternative delivery method might reduce these problems.
1.6.2. Replacement of miRNA
In situations of reduced miRNA expression in certain diseases, re-introduction of
the miRNA could be advantageous. One method of re-introducing miRNA is through
miRNA mimetics or miRNA expressing vectors. miRNA mimetics contain endogenous
17
mature miRNA sequence in a duplex structure. The duplex structure is necessary
because RISC is more efficient at loading small RNAs from double-stranded RNAs
rather than a single-stranded RNAs (130). RISC will also process the duplex to release
the single stranded mature miRNA. miRNA mimetics successfully inhibit Gli-1
expression and modified miRNA mimetics could show better effect than endogenous
miRNA (131).
An alternative method to replace miRNA is through the use of short hairpin RNA
(shRNA) vectors. This vector can maintain silencing effect for a long period of time and
more than one miRNA transcripts can be expressed from the same vector. shRNA may
introduce enough pre-miRNA, to cause saturation of the Exportin-5 pathway (132).
Modified adenovirus have been used, but problems with immune response to the
adenovirus and available tissues that virus can efficiently infect limit the usage of this
virus (133). Lentivirus also have been studied for shRNA vector and this produces more
stable and persistent silencing effect because it incorporates into the genome (134).
However, lentivirus shows the possibility of insertional mutagenesis and wild-type virus
production (134).
1.7 OJECTIVE OF DISSERTAION
The first aim of this study is to identify the most important miRNAs that are
deregulated in pancreatic cancer. Theoretically, these deregulated miRNAs may be used
for diagnosis or treatment of pancreatic cancer. The expression of miRNA precursors
was investigated in pancreatic tissues and cancer cell lines (Chapter 2). Overexpressed
18
miRNAs in pancreatic cancer were inhibited using antisense oligonucleotides (Chapter 3
and 4) and the effects of combining antisense oligonucleotides to miRNA and commonly
used a chemotherapeutic agent was studied (Chapter 3). The target molecules of
miRNAs that are deregulated in pancreatic cancer were studied in Chapter 4.
The second aim was to see if the processing of miRNA differed in various normal
tissues, cancer cell lines and in patients’ tumor tissue (Chapter 5).
19
Figure 1.1. miRNA biogenesis.
miRNA gene Pol II
Drosha/DGCR8
cap AAA….A Pri-miRNA
Nucleus
Cytoplasm
Pre-miRNA Exportin-5
Pre-miRNA
Dicer Dicer/TRBP/Ago
Mature miRNA in RISC
20
Figure 1.2. Chemical modification of antisense oligonucleotides. (A): 2’-MOE (2’-O-methoxyethyl), (B): 2’-OMe (2’-O-methyl), (C): 2’-F, (D): LNA,
(E): phosphorothioate backbone, (F): phosphodiester backbone.
21
CHAPTER 2
EXPRESSION PROFILING IDENTIFIES MICRORNA SIGNATURE IN
PANCREATIC CANCER
2.1 INTRODUCTION
Pancreatic cancer is the fourth leading cause of cancer-related death in the United
States (135). The annual death rate over the last five years has been ~30,000 with a
similar number of new cases diagnosed each year. The prognosis for pancreatic cancer is
the worst of all cancers with a mortality/incidence ratio of 0.99 (136). The incidence of
pancreatic cancer in the United States is ~9 per 100,000 (137). These discouraging
numbers, reflecting the increasing rates of incidence and death, are due to the lack of
improvement in detection and diagnosis strategies and the paucity of breakthroughs in
treatment regimens.
A growing number of direct and indirect evidence suggests a relationship between
altered miRNA expression and cancer. These include miR-15a and miR-16-1 in chronic
lymphocytic leukemia (56, 113), miR-143 and miR-145 in colorectal cancer (76), let-7 in
lung cancer (79, 97) and miR-155 in diffuse large B cell lymphoma (91). Expression
profiling has identified other cancers with differential expression of several miRNAs,
22
including breast cancer (99), glioblastoma (87, 100) and papillary thyroid cancer (90). A
polycistron encoding five miRNAs is amplified in human B-cell lymphomas and forced
expression of the polycistron along with c-myc was tumorigenic, suggesting that this
group of miRNAs may function as oncogenes (94, 95).
The purpose of this study was to profile the miRNA expression in clinical
specimens of pancreatic adenocarcinoma. A real-time, quantitative PCR assay (59, 61)
was used to profile the expression of over 200 miRNA precursors in clinical specimens of
pancreatic cancer and pancreatic cancer cell lines. A unique miRNA signature was
identified that distinguished pancreatic cancer from normal and benign pancreas.
2.2 MATERIAL AND METHODS
2.2.1 Tissue procurement
The tissue samples analyzed in this study were derived from patients undergoing a
surgical procedure to remove a portion of the pancreas at the University of Oklahoma
Health Sciences Center and The Ohio State University. The collection of samples
conformed to the policies and practices of the facility’s Institutional Review Board.
Upon removal of the surgical specimen, research personnel immediately transported the
tissue to the surgical pathology lab. Pathology faculty performed a gross analysis of the
specimen and selected cancerous appearing pancreatic tissue and normal appearing
pancreatic tissue for research. Each sample was placed in a cryovial and flash-frozen in
liquid nitrogen and stored at -150°C until analysis. Subsequent pathologic analysis by
the institutes providing the surgical specimens confirmed the histopathology of the
23
samples taken for research. A second level of quality control was performed on the
adjacent benign tissues by the laboratory that performed the RNA analysis. Histological
slides were prepared from the section of the frozen tissue directly adjacent to tissue from
which RNA was isolated. These slides were examined by one of us (W.L.F.) to
determine if the benign tissues contained any pancreatic tumor cells. Benign tissue that
contained residual tumor was not included in the study. The clinical data on the
specimens are listed in Table 2.1.
2.2.2 Cell lines
The following pancreatic tumor cell lines were purchased from American Type
Tissue Collection (Manassas, VA), which are Panc-1, HS766T, MIA PaCa-2, HPAF-II,
BxPC-3, Mpanc-96, PL45, Panc03.27 and Panc10.05. Cell lines were cultured in RPMI
1640 medium with 10% FBS or other optimized complete medium using standard
conditions.
2.2.3 miRNA precursor expression profiling
Total RNA was isolated from the cell lines or tissues in 1 ml of Trizol (Invitrogen,
Carslbad, CA). Frozen tissues (~10 mg) were first pulverized in a stainless steel mortar
and pestle. Total RNA from normal pancreases were purchased from Ambion (Austin,
TX), BD Biosciences (Mountain View, CA) and Stratagene (La Jolla, CA). All donors of
the normal tissue died from complications other than pancreatic diseases (Table 2.1).
RNA concentration was determined by analyzing 1 µl of solution using the ND-1000
micro-spectrophotometer (NanoDrop Technologies, Wilmington, DE). RNA integrity
24
was evaluated using the Agilent 2100 Bioanalyzer (Agilent Technologies, Palo Alto, CA).
RNA integrity number (RIN) was determined using the RIN algorithm of the Agilent
2100 expert software (138) (Table 2.1). RNA with a RIN ≥ 4 was included in the study.
RNA was briefly treated with RNase-free DNAase I and cDNA was synthesized from 1
µg of total RNA using gene specific primers to 222 miRNA precursors plus 18S rRNA as
described (59, 61). The expression of 222 miRNA precursors was profiled using a real-
time quantitative PCR assay (59, 61). Duplicate PCRs were performed for each miRNA
precursor gene in each sample of cDNA. The mean CT was determined from the
duplicate PCRs. Relative gene expression was calculated as 2 — (C
TmiRNA— C
T18SrRNA).
Relative gene expression was multiplied by 106 to simplify the presentation of the data.
The raw relative miRNA precursor expression data are provided in Appendix Table A.
2.2.4 Real-time PCR of mature miRNA
Assays to quantify the mature miRNA (i.e. TaqMan®
microRNA Assays, Applied
Biosystems Foster City, CA) were conducted as described (60) with one modification. A
5X cocktail containing 28 different antisense looped RT primers was prepared by
concentrating the 2X stock solutions in a Speed Vac. One hundred nanograms of total
RNA was heated for 5 min at 80°C and then incubated for 5 min at 60°C with 10 µM of
the 18S rRNA antisense primer followed by cooling to room temperature. Three
microliters of the 5X looped primer mix was then added and the cDNA was made as
described (60). This allowed for the creation of a library of 28 miRNA cDNAs plus the
18S rRNA internal control. Real-time PCR (10 µl total reaction) was performed as
described (60) using 1 µl of a 1:50 dilution of cDNA. Duplicate PCRs were performed
25
for each mature miRNA gene in each sample of cDNA. The mean CT was determined
from the duplicate PCRs. Gene expression was calculated relative to 18S rRNA as
described earlier and multiplied by 106 to simplify data presentation.
2.2.5 Northern blotting
Northern blotting was performed as previously described (2, 59). DNA
oligonucleotides of the reverse compliment to the mature miRNA were used as probes.
Blots were successfully stripped and reprobed up to 3 times.
2.2.6 RT in situ PCR
The RT in situ PCR protocol was performed as previously described (139). Briefly,
optimal protease digestion time was determined using nonspecific incorporation of the
reporter nucleotide digoxigenin dUTP. Optimal protease digestion was followed by
overnight incubation in RNase-free DNase (10 U per sample, Boehringer Mannheim,
Indianapolis, IN) and one step RT/PCR using the rTth system and digoxigenin dUTP.
The chromogen is nitroblue tetrazolium and bromochloroindolyl phosphate (NBT/BCIP)
with nuclear fast red as the counterstain. The primer sequences to the precursors of miR-
221, miR-301 and miR-376a were the same as those used for the profiling (61). The
negative controls included omission of the primers and substitution with irrelevant
(human papillomavirus specific) primers, as this virus does not infect pancreatic tissue.
RT in situ PCR was performed on the archived, formalin-fixed paraffin-embedded
sample 1050005A2(T) (Table 2.1).
26
2.2.7 Statistics
The ∆CT data for 201 gene expression values (relative to control gene expression)
were mean-centered and analyzed using the following strategy. The expression patterns
of unfiltered data were assessed using unsupervised hierarchical clustering of samples
and unsupervised hierarchical clustering of genes based on average linkage and Euclidian
distance (140, 141). To determine genes that are differentially expressed between groups
of samples, the data were filtered on significance of differences using multi-group
permutations-based ANOVA test (Welch approximation) with p < 0.01 (10,000 random
permutations) and multiple testing correction (Westfall-Young step-down correction with
maxT). To compare the expression patterns of differentially expressed genes, the filtered
data were analyzed using hierarchical clustering of samples and hierarchical clustering of
genes based on average linkage and Euclidian distance (140, 141).
Additional cluster analysis of filtered data was done using an expression terrain
map (142). Terrain maps provide a three-dimensional overview of the major clusters
inherent in the data. Samples were first mapped into a two-dimensional grid in which the
placement of each element is influenced by a number of nearest neighbors based on
Euclidian distance that was calculated using miRNA precursor expression data. The third
dimension is determined by the density of points over the two-dimensional grid and its’
value is projected as a surface; higher peaks indicate larger numbers of very similar
elements. The average correlation between each pair of samples was also calculated.
Each pair of samples with an average correlation above the threshold (0.8) is indicated on
the expression terrain map by a line connecting the two samples. This allows visualizing
subsets of samples with a highly correlated pattern of miRNA expression. Peaks
27
representing samples from a particular cluster are labeled with color coded spheres on top
of each peak.
A supervised machine learning algorithm was applied for classification of
samples based on unfiltered miRNA expression data. The predictive scores for each
miRNA were calculated based on 2 class comparison (normal vs. tumor) of expression
data using the prediction analysis of micro-arrays algorithm (PAM) (143) based on
training, take-one-out cross validation and testing procedures. The division of the
samples into training and test sets is done using the commonly accepted approach of
randomly splitting data into training and test sets (usually 2/3 for train, 1/3 for test) (143).
For this study, tumor samples were randomly split into ~75% for training and 25% for
testing. Since the number of normal cases was small (N = 6), normal cases were included
in the training set only. The small number of chronic pancreatitis samples (N = 4) were
not included in the PAM analysis. While, two levels of quality control were performed to
eliminate adjacent benign tissue that contained any tumor cells, the RNA was extracted
from whole tissue rather than microdissected tissue. Thus, we could not completely rule
out the possibility that some tumor cells contaminated the adjacent benign, nor do we
know if early premalignant changes have occurred in these benign samples that are
obtained from tissue adjacent to tumor. Therefore benign samples were excluded from
the training set in order to train the classifier on true normal and tumor cases. Benign
cases were used in the test set.
28
2.3 RESULTS
2.3.1 Validation
Pancreatic tissue is rich in ribonuclease and care must be taken during RNA
isolation to reduce the possibility of autolysis. To validate the integrity of the RNA
isolated from the pancreatic tissue, ~100 ng of each RNA sample was assayed using the
Agilent 2100 Bioanalyzer. Fifty-two tissues had a RIN ≥ 4 (median 7.6, range 4.3–9.6,
Table 2.1).
miRNA precursor expression in each of the samples was determined using real-
time PCR and normalized to an internal control gene. Since equivalent amounts of total
RNA was added to each RT reaction, 18S rRNA was validated as the internal control by
comparing the mean expression among the various samples (i.e. tumor, normal and
benign). There was no statistically significant difference in the mean 18S rRNA
expression between the tumor samples or the normal pancreas (p = 0.116, Figure 2.1).
The 18S rRNA expression in the tumor and normal tissue determined here are in
agreement with those previously reported in pancreatic tissue (144). Since there was no
significant difference in the 18S rRNA expression between the tumor, normal and benign
groups, 18S rRNA was selected as the internal control gene in the study.
2.3.2 miRNA precursor profiling in pancreatic tissues
The expression of 201 miRNA precursors, representing the 222 miRNAs
discovered as of April, 2005 was profiled in 28 tumors, 15 adjacent benign tissues, 4
chronic pancreatitis specimens, 6 normal pancreas tissues and 9 pancreatic cancer cell
29
lines. Unsupervised hierarchical clustering of samples was performed on the entire set of
unfiltered data. The heatmap demonstrates that unfiltered expression data of only 201
miRNA precursors sufficiently sorts the samples into clusters of normal pancreas, tumor
and cell lines (Appendix Figure A). While most of the benign samples clustered together
with the normal pancreas samples, several benign samples clustered with the tumor
samples or pancreatitis.
The miRNA precursor expression data was filtered using multigroup ANOVA.
Statistical filtering of data is conventionally considered to be a required step of the
expression data preprocessing because gene expression data are inherently noisy.
Hierarchical clustering of samples and genes was performed on the resulting 112
miRNAs. Hierarchical clustering of filtered data allowed us to identify major groups of
miRNAs that have different patterns of expression in the resulting four clusters of
samples (Figure 2.2 a,b). One cluster contained only cell lines. Another cluster
contained all 6 normal pancreases and 9 of 15 benign tissues. A third cluster contained
the 4 chronic pancreatitis specimens and 1 benign tissue. Finally, a large cluster
contained 28 of 28 tumors and 5 benign tissues (Figure 2.2 a,b).
The filtered data were also analyzed using a different clustering technique known
as expression terrain maps (142). The terrain map separated the expression data into 5
main groups of samples (Figure 2.3). Like the hierarchical clustering, the terrain map
separates groups of samples from the normal pancreas, the cell lines, pancreatic
adenocarcinomas and chronic pancreatitis. This analysis showed that each of the clusters
of samples occupies distinct regions on the expression terrain map thus providing
additional evidence that each of the 4 groups of samples has distinct patterns of miRNA
30
expression and suggests the possibility of finding subsets of microRNAs that discriminate
normal and tumor samples. Most of the adjacent benign tissue grouped in between the
normal and tumor, while those benign samples that clustered with the tumor also grouped
with the tumor on the terrain map (Figure 2.2 and 2.3). As an additional feature of terrain
map, the average correlations of all miRNA expression values were calculated between
each pair of samples. Those correlations above the threshold of 0.8 are shown as lines
connecting pairs of samples with similar patterns of miRNA expression. This allows
visualizing subsets of samples with a highly correlated pattern of miRNA expression.
The samples within each of the 4 main groups are connected (i.e. average correlation
>0.8) but there were no between the groups connections, except for two pancreatitis
samples that correlated with some of the tumor samples (Figure 2.3).
Comparing the gene expression profile among different pancreas tissues has been
used to eliminate the stroma and cell proliferation (e.g. cell line) contributions and
identify genes expressed in pancreatic tumors (145-147). An attempt to perform such
analysis on the miRNA expression data in Figure 2.2a was unsuccessful due to the
uniformly high expression in the cancer cell lines and the uniformly low expression in the
pancreatitis samples. However, a number of possible tumor-related miRNAs were
identified that were increased in the cell lines or tumors but not in the normal or
pancreatitis (Figure 2.2a).
2.3.3 Data analysis by prediction analysis of micro-array algorithm (PAM)
The PAM classification algorithm was used to determine if the miRNA
expression data could predict which class the samples fit (tumor or normal) and to
31
determine the most important, differentially expressed miRNAs related to pancreatic
adenocarcinoma. The unfiltered data on 201 pre-miRNAs were analyzed by the PAM
algorithm (143). The 3 genes with more than 75% of missing data were eliminated from
the analysis in order to prevent possible artifacts of the imputation algorithm. PAM
training and cross-validation were conducted using the 6 normal pancreas and 18
pancreatic tumors. PAM has correctly classified 100% of the normal and tumor samples
(Figure 2.4a). PAM testing was conducted on 10 tumor samples and 15 adjacent benign
samples. PAM has correctly classified 100% of the tested tumor samples and 11 of 15 of
the tested benign samples (Figure 2.4b). The 20 top ranked differentially expressed
miRNAs in pancreatic adenocarcinoma as selected by PAM are listed in Table 2.2.
2.3.4 Validation of miRNA expression
The real-time PCR assay used in this study quantifies the miRNA precursors and
not the active, mature miRNA (59, 61). Northern blotting was performed on the identical
RNA used in the real-time PCR analysis to validate if the mature miRNA correlated with
the precursor. Expression levels of mature miR-100, mir-375and miR-155 from 4 normal
pancreases and 5 pairs of tumor/adjacent benign tissue paralleled the miRNA precursor
levels by PCR (Figure 2.5). miR-100 and miR-155 were among the top 20 differentially
expressed miRNAs (Table 2.2). miR-375 was validated by Northern blotting because it
was one of the few miRNAs with decreased expression in pancreas cancer. While miR-
375 was not among the top 20 miRNAs (Table 2.2), the expression of both precursor and
mature miR-375 was significantly decreased in pancreas cancer by real-time PCR (p < 1
X 10-5
). miRNA expression in the paired benign and tumor tissue was consistently
32
increased or decreased in all cases, demonstrating that the differences in miRNA
expression between tumor and benign are due to differences in individual patient’s tissues
and not due to differences in the mean expression of the group.
The real-time PCR data was further validated using a commercially available real-
time PCR assay to amplify and quantify the mature miRNA. Mature miRNA expression
was validated on 8 of the top aberrantly expressed miRNAs from PAM. cDNA from the
following tissues were assayed: 6 normal pancreases, 16 pancreatic adenocarcinomas and
10 adjacent benign tissues that were predicted as normal from PAM. The mature miRNA
expression highly correlated with the miRNA precursor (Figure 2.6). To our knowledge,
this is the initial presentation of both precursor and mature miRNA expression
determined by sensitive, real-time PCR assays. A direct comparison between the
precursor and mature PCR data presented in Figure 2.6 is not possible. Among other
reasons, different amounts of RNA were added to each RT, 1 µg for the precursor assay
and 100 ng for the mature. Despite these differences, the relative amount of mature
miRNA is greater than the precursors in all cases except for panel H. This demonstrates
that the steady state levels of mature miRNA are more predominant than the precursors, a
fact that is documented on nearly all published Northern blots that show the band
intensity of the mature is more intense than the precursor. We have no explanation as to
why this situation is apparently reversed in the case of panel H (miR-212). Of interest is
that while the relative expression values for these mature miRNAs spanned 3-logs (from
0.1 to 100), the trend in differential expression between the tumor and normal tissues
remained (Figure 2.6). This suggests that miRNAs function in tumor and normal
33
pancreas at different expression levels, yet the differential expression is maintained
between cancer and normal tissue.
Finally, the cell-type miRNA expression was studied using RT in situ PCR. miR-
221, miR-376a and miR-301 were selected for the in situ PCR since they were among the
top differentially expressed miRNAs (Table 2.2) and had increased expression in the
tumor and cell lines compared to the normal pancreas and pancreatitis (Figure 2.2). We
were particularly interested in the cell type expression of miR-376a since it was cloned
from pancreas cells (67). miR-221 and miR-376a are localized to the tumor cells and not
to the benign pancreatic acini or stromal cells (Figure 2.7) or benign ducts (not shown).
miR-301 was also localized to tumor (data not shown).
2.4 DISCUSSION
Reported here are the results of the first detailed miRNA expression profiling
study in pancreatic ductal adenocarcinoma. Expression profiling identified a large
number of miRNAs that are aberrantly expressed in pancreatic ductal adenocarcinoma.
A bead-based flow cytometric assay was used previously to profile the expression of 217
miRNAs in nine samples of pancreatic adenocarcinoma (65). A micro chip assay was
recently used to profile the expression of miRNAs in 39 samples of endocrine pancreas
cancer (148). Our results in pancreatic ductal adenocarcinoma more closely approximate
those of Volinia, et al. (148), in endocrine pancreas tumors and show that the majority of
miRNAs are increased in the tumor compared to normal pancreas. Many of the miRNAs
that are increased in both pancreas adenocarcinoma and endocrine pancreas cancer are
34
similar, including miR-221, -100, -125b and -21. On the other hand, Lu, et al. (65),
report an almost universal decrease in miRNA expression in the pancreatic
adenocarcinomas compared to normal pancreas.
miRNAs are believed to function primarily as negative regulators of gene
expression following binding to conserved sequences within the 3’ UTR of target
mRNAs. While the biological roles of miRNA are under intense investigation, they are
believed to define and maintain cellular fate in a manner similar to transcription factors
(149) by regulating developmental timing and differentiation (150). Since alterations in
developmental pathways play a critical role in pancreatic cancer development (151, 152),
alterations in miRNA expression may be an important contributor to the development of
pancreatic adenocarcinoma.
Our study is unique in that a sensitive, real-time PCR assay was used to profile a
relatively small number of nonconding RNAs. Real-time PCR is the gold standard of
RNA quantification and has much less technical noise and greater reproducibility than
traditional cDNA micro-arrays. miRNA expression profiling correctly identified 28 of 28
tissues as tumor (Figure 2.4). All 6 normal pancreases were correctly predicted and 11 of
15 adjacent benign tissues were classified as normal tissue (Figure 2.4). The data
presented here reinforces the argument that miRNA expression profiling may generate a
unique molecular signature for a given cancer. This concept is supported by several
recent studies. Profiling of various cancers revealed that the pattern of miRNA
expression varies markedly across different tumors and that a small number of miRNAs
define the cancer better than expression data from 16,000 mRNAs (65). A unique
expression signature of only 13 miRNAs differentiated cases of the more aggressive form
35
of chronic lymphocytic leukemia from the more indolent form and was associated with
the presence or absence of disease progression (153).
The 3 factors that are likely driving the differences in gene expression are the
normal acini, stroma and tumor cells. We cannot conclude that each of the aberrantly
expressed miRNAs (Table 2.2) reflect a difference in expression between normal ductal
epithelium and tumor and may reflect differences in expression among the different cell
types (stoma, acini and tumor). However, we confirmed by RT in situ PCR that 3 of the
top differentially expressed miRNAs that were identified in the screen are localized to the
tumor cells (Figure 2.7). We cannot explain why some of the benign tissues failed to
cluster with the normal pancreas (Figure 2.2 and 2.3). While 2 levels of quality control
were used to reduce the possibility of contaminating tumor cells, it is possible that some
tumor cells were present in the benign tissue since the RNA was isolated from whole
tissue and not microdissected tissue. Another possibility is that premalignant changes
have already occurred in some of the benign tissues as those samples are obtained from
tissue adjacent to tumor. It is interesting that most of the benign samples (and chronic
pancreatitis as well) lie in between the normal pancreas and tumor on the expression
terrain map (Figure 2.3). This observation may indeed describe the premalignant
alterations that have occurred in these benign tissues. Future studies using RT in situ
PCR and perhaps laser microdissection will be able to address these issues in more detail.
Some of the differentially expressed miRNAs in pancreatic cancer were aberrantly
expressed in other cancers. These include miR-155, which was increased in the present
study and in diffuse large B-cell lymphoma (91); miR-21 was increased here and in
glioblastomas (87, 100), breast cancer (99) and papillary thyroid cancer (90); miR-221
36
was increased in pancreatic cancer, in glioblastoma (100) and in thyroid cancer (90).
miR-221 is located ~700 bp from miR-222 on the X chromosome; both miR-221 and
miR-222 are predicted to bind to and regulate kit (90). miR-222 precursor was not
among the top 20 differentially expressed miRNAs (Table 2.2); however, subsequent
analysis of mature miR-222 by PCR showed that miR-222 was increased in pancreas
cancer at levels that were similar to miR-221 (data not shown). Thus, deregulation of the
miRNAs mentioned earlier may be unique to cancer in general. miRNAs differentially
expressed in other cancers were not deregulated to the same degree in pancreatic cancer.
The let-7 family, decreased in lung cancer (79, 97), was increased here. Expression of
the miR-17-92 polycistron (encoding miR-17, -18, -19a, -19b-1 and -92-1) was increased
in lymphoma and colorectal cancer (94) but was not significantly altered in pancreatic
cancer. We report deregulation of a number of miRNAs in pancreatic cancer such as
miR-376a and miR-301 that have not been reported in any other cancers to our
knowledge. Also of interest is the fact that most of the deregulated miRNAs reported
here show increased expression in the tumors compared to the normal pancreas. A few
miRNAs had reduced expression in pancreatic cancer including miR-375 (Figure 2.5).
miR-375 was cloned from pancreas and is believed to be islet cell specific (67).
We report significant changes in miRNA expression between pancreatic
adenocarcinoma and normal pancreas. Since each miRNA may regulate scores of
mRNAs (45), the impact on gene expression in pancreatic cancer may be profound. The
miRNA field is currently hampered by a lack of methods to sort through the many
hundreds of predicted miRNA target genes. As more sophisticated approaches become
37
available to identify and validate miRNA targets, the role of aberrant miRNA expression
in pancreas cancer will become better understood.
2.5 ACKNOWLEDGEMENTS
Supported by an NIH grant CA107435 to T.D.S. The Tissue Procurement Shared
Resource at The Ohio State University is funded by the National Cancer Institute, grant
P30 CA16058. We thank Dr. Caifu Chen at Applied Biosystems for his assistance with
the TaqMan looped primer assays.
38
Figure 2.1. 18S rRNA expression in pancreatic tissue.
The expression of the 18S rRNA internal control is shown in pancreatic tumors, adjacent
benign tissue, normal pancreas, chronic pancreatitis and pancreatic cancer cell lines. 18S
rRNA expression, determined using real-time PCR as described in Material and Methods,
is presented as 2-CT
. Dashed line, mean value.
39
Figure 2.2. miRNA precursor expression in pancreatic samples.
(a) The relative expression of each miRNA precursor was determined by real-time PCR;
data are presented as ∆CT. Hierarchical clustering was performed on a subset of 112
genes that are differentially expressed (p < 0.001) among groups (tumor, chronic
pancreatitis, cell lines and normal tissue) as determined by ANOVA multi-group
comparison test. A median expression value equal to 1 was designated black; red,
increased expression; green, reduced expression; grey, undetectable expression.
(b) Dendrogram representing the results of hierarchical clustering analysis of the miRNA
precursor expression pattern in 62 samples. Samples include primary pancreatic tumors
(N = 28), normal pancreatic tissues (N = 6), adjacent benign pancreas (N = 15), chronic
pancreatitis (N = 4) and pancreatic cancer cell lines (N = 9).
40
Figure 2.3. Three-dimensional expression terrain map.
It was created from the filtered miRNA precursor expression data presented in Figure 2.2
(a). Each mountain represents an individual sample (tumor, adjacent benign, chronic
pancreatitis, normal pancreas or pancreatic cancer cell line). The individual mountains
sort into small groups based upon their similarities or differences to each other. Colored
dots represent the same clusters as in Figure 2.2 (b). Black dots represent the 6 normal
pancreases. The lines connecting pairs of samples indicate those samples which have
very similar patterns of miRNA expression with average correlation above the threshold
(>0.8).
41
Figure 2.4. Estimated probabilities for the training and test data. All training data including 6 normal pancreas samples and 18 of the samples known to be
pancreatic tumors are correctly classified (a). Eleven out of 15 adjacent benign samples
and 10 samples known to be pancreatic tumors are correctly classified in the testing
group (b). Samples are partitioned by the true class (a) and the predicted class (b).
42
Figure 2.5. miRNA expression by Northern blotting.
The expression of miR-100, miR-375 and miR-155 was determined in tissue specimens
of pancreatic cancer (T), adjacent benign tissue (B) or normal pancreas (N). Blots were
stripped and reprobed. tRNA, visualized by ethidium bromide staining, was used as a
loading control.
43
Figure 2.6. Validation of precursor and mature miRNA levels.
The expression of 8 miRNAs was validated in 6 normal pancreas specimens, 10 adjacent
benign tissues and 16 pancreatic adenocarcinomas. The relative expression of the
miRNA precursors (open bars) was determined using a real-time PCR assay to the
miRNA precursors while the relative expression of the mature miRNA (closed bars) was
determined using a real-time PCR assay to the mature miRNAs. The mean differences in
miRNA expression between the normal pancreas (black) and tumors (red) was significant
p < 0.01 (student’s t-test). (a) let-7i, (b) miR-221, (c) miR-100, (d) miR-301, (e) miR-21,
(f) miR-181a,c (precursor) and miR-181a (mature), (g) miR-125b-1 (precursor) and miR-
125b (mature), (h) miR-212.
44
45
Figure 2.7. Histologic and molecular analyses of pancreatic cancer for microRNA
expression.
Panel (a) (X400) depicts the hematoxylin and eosin analysis of a pancreatic
adenocarcinoma. The normal pancreatic glands (small arrow) are being invaded by the
poorly formed glands of the carcinoma (large arrow). Serial section analysis of miR-221
after in situ amplification of the corresponding cDNA showed that many of the tumor
cells contained the target sequence; note the cytoplasmic localization (arrows, panel (b) –
X 400 and at higher magnification, panel (c) – X 1,000; the signal is blue due to
NBT/BCIP with negative cells counterstained with fast red). The signal was lost with
either omission of the primers or substitution with HPV-specific primers (panel (d),
X400). The adjacent serial section also showed many of the tumor cells expressed miR-
376a after in situ amplification of the cDNA (e, f). Panel (e) (X400) shows the positive
tumor cells (large arrow) and the negative stromal cells in the areas of desmoplasia (small
arrow) while panel (f) (X400) depicts the positive tumor cells (large arrow) adjacent to
the negative benign pancreatic gland acini (small arrow).
46
Normal Pancreas Tissues
Sample No. Supplier Age (years)/Gender Cause of Death RINa
N1 Ambion 82 / Female Emphysema 9.6
N2 Ambion 20 / Male Head Trauma 7.8
N3 Ambion 71 / Male Cerebral Vascular Accident 9.2
N4 Ambion 71 / Male Failure to Thrive 9.1
BD Clontech 35 / Male Sudden death 7.5
ST Stratagene 72 / Male Chronic Obstructive Pulmonary Disease 6.8
Pancreatic Adenocarcinoma Tissues
Sample No. Tumor Pathology Differentiatio
n
Age
(years)/Gender
RIN
(tumor)
RIN
(benign)
OUP2 T3NxMx Moderately 85 / Male 8.2 7.0
OUP14 T3N0Mx invasive Poor-moderate 71 / Male 7.8 3.8
OUP16 T3N1bMx Well 50 / Male 5.6 5.6
OUP20 T3N1aMx Poorly 70 / Male 8.2 3.4
OUP27 T3N0Mx invasive Moderate 86 / Male 3.3 5.8
OUP29 T3N0Mx invasive Poorly 63 / Male 8.3 7.0
OUP28 T3N1bMx invasive Poorly 48 / Female 7.9 6.9
OUP31 T3N0Mx Poorly 38 / Male 7.7 7.0
OUP33 T3N1Mx Poor-moderate 42 / Female 5.1 8.3
OUP36 T3N1bMx Moderate 56 / Female 7.7 7.8b
OUP37 T3N1bMx invasive Mod-poor 79 / Male 7.1 No tissue
OUP42 T3N0Mx invasive Moderate 78 / Female 6.6 7.6
OUP48 T3N1bMx invasive Moderate 61 / Male 8.1 4.3
OUP49 T3N1aMx invasive Poor-moderate 62 / Female 7.6 8.6
OUP91 T3N1bMx Moderate 65 / Male 5.9 6.1
OUP97 T3N1bMx Moderate 56 / Male 8.0 2.6
OUP101 T2N0Mx invasive Moderate 52 / Female 8.5 6.6
OUP105 T2N1aMx Moderate 55 / Male 8.3 8.9
OUP122 T3N1aMx Moderate 64 / Male 9.1 8.4
OUP123 T3N1bMx Moderate-well 52 / Male 6.4 7.6 b
OUP135 T3N0Mx Moderate 82 / Male 7.8 8.2 b
OUP139 T2N0Mx Moderate 50 / Male 6.6 6.7
0206C077B(T) T3N0Mx Moderately 49 / Male 7.2 No tissue
0206C138B(T) T3N0M1 Intermediate to
high grade 44 / Female 6.1 No tissue
1050005A2(T) T3N1Mx Poorly 73 / Female 7.0 7.3
3030800A3(T) T3N1Mx Moderately 46 / Male 7.5 No tissue
3030396A1(T)
Intraductal
papillary mucinous
tumor
- 59 / Male 7.3 No tissue
3030401A1(T)
Intraductal
papillary mucinous
tumor
- 72 / Male 8.9 No tissue
Table 2.1. Clinical data and tumor pathology.
47
Table 2.2. Top 20 aberrantly expressed miRNA precursors in pancreatic
adenocarcinoma.
Rank Name p-value
(t-test)
Fold
Change
Chromosome
location
1 miR-221 5.66E-05 26.2 Xp11.3
2 miR-424 3.62E-08 56.3 Xq26.2
3 miR-301 1.11E-05 34.2 17q23.2
4 miR-100 4.40E-06 36.9 11q24.1
5 miR-376 7.00E-04 7.79 14q32.31
6 miR-125b-1 1.00E-04 23.2 11q24.1
7 miR-021 2.00E-04 15.7 17q23.2
8 miR-345 1.44E-15 -14.5 14q32.2
9 miR-016-1 3.73E-04 14.3 13q14.2
10 miR-181a,c 8.31E-04 18.6 9q33.3, 19p13.13
11 miR-092-1 3.40E-03 19.6 13q31.3
12 miR-015b 4.00E-04 8.55 3q25.33
13 miR-142-P 3.63E-07 -15.4 17q23.2
14 miR-155 1.51E-03 14.0 21q21
15 let-7f-1 4.00E-04 10.9 9q22.32
16 miR-212 2.00E-04 22.2 17p13.3
17 miR-107 3.86E-05 8.20 10q23.31
18 miR-024-1,2 9.12E-08 8.17 9q22.32, 19p13
19 let-7d 7.06E-04 8.38 9q22.32
20 miR-139 6.79E-11 -7.91 11q13.4
48
CHAPTER 3
ANTISENSE INHIBITION OF microRNA REDUCES CELL VIABILITY AND
SENSITIZES THE EFFECT OF GEMCITABINE IN PANCREATIC CANCER
3.1 INTRODUCTION
Pancreatic cancer is the fourth leading cause of cancer-related death in the United
States (135). The annual death rate over the last five years has been approximately
30,000 with a similar number of new cases diagnosed each year. The prognosis for
pancreatic cancer is the worst of all cancers with a mortality/incidence ratio of 0.99. The
incidence of pancreatic cancer in the United States is approximately 9 per 100,000 (136,
137). Pancreatic ductal adenocarcinoma (PDA) is the most common and lethal type of
pancreatic cancer. The reasons for the poor survival rate are a lack of effective diagnostic
procedure to identify pancreatic cancer and a lack of effective treatments for the disease.
Normal pancreatic tissue consists primarily of acinar cells, which produce
digestive proenzymes, islets, which produce hormones that control blood sugar such as
insulin and glucagon, and a low number of ductal epithelial cells. However, if pancreatic
tissue turns to tumor, the major cell type is neoplastic ductal epithelial cells and stroma
cells.
49
Not many sensitive and specific biomarkers of pancreatic cancer are available.
Currently serum maker CA19-9 (sialylated Lewis blood group antigen) is used, but the
usage is limited because it showed the high false-positive rate and some patients do not
express Lewis antigens (154-156). Recently, serum macrophage inhibitory cytokine 1
(MIC-1) seems to be a better marker of pancreatic cancer than CA19-9 (157). Genetic
and epigenetic markers also can be used for early detection. Pancreatic cancer is caused
by genetic alterations such as chromosomal instability or point mutation of oncogenes
and tumor-suppressor genes. Chromosomal changes in pancreatic cancer have been
identified using karyotyping, comparative genomic hybridization, and allelotyping (158).
Chromosome losses are more common than gains and poorly differentiated ductal
adenocarcinoma showed more frequent karyotypic abnormalities than well-differentiated
pancreatic cancer (159). The specific oncogenes and tumor suppressor genes are also
altered in pancreatic cancer. Point mutations in K-ras oncogene occur in 85-95% of
pancreatic cancers (160) and inactivation of tumor-suppressor genes such as p16, p53 or
SMAD4 occurs in 98%, 75%, and 55% of pancreatic cancers, respectively (158).
Oncogenes and tumor-suppressor genes are also involved in cell cycle arrest and
apoptosis. BRCA2, TGF-β, BAX mutations are found in pancreatic cancers. Several
tumor suppressor genes such as PENK, RARB, CDKN2A, CACNAIG, TIMP3, CDH1
AND MLH1 are often inactivated by CpG island hypermethylation (161).
The prognosis of pancreatic cancer is poor. If patients with pancreatic cancer are
diagnosed early, surgical resection is available and the 5-year survival rate in patients
who had surgery treatment is 21% (162). However, for patients with unresectable
pancreatic cancer, the 2-year survival rate is 5%–18%, and the 5-year survival rate is less
50
than 5% (163-165). For advanced pancreatic cancer, chemotherapy or radiation
treatment, or a combination of two can be applied, however, pancreatic cancer is highly
resistant to chemotherapy and radiation treatment (166, 167).
5-Fluorouracil (5-FU) treatment was the standard treatment for pancreatic cancer
(166). Gemcitabine is currently used for the first choice of pancreatic cancer treatment.
Gemcitabine is an analog of deoxycytidine (Figure 3.1) and it blocks DNA elongation,
causing DNA fragmentation and cell death (168). Gemcitabine is a prodrug and it is
phosphorylated by deoxycytidine kinase to make gemcitabine monophosphate and then it
is converted to active, di- and triphosphate (169). This gemcitabine triphosphate is
incorporated into DNA chain and terminates DNA synthesis. Therefore, gemcitabine
shows cell killing effect at S-phase of cell cycle. Reproducible clinical benefit of
gemcitabine was 23.8%, which is higher than 4.8% in 5-FU and the 1-year survival in
gemcitabine treated patients (18%) was also higher than in 5-FU treated patients (2%)
(170). Even though gemcitabine shows better effect than 5-FU, it is still marginally
effective. The combination therapy with other anticancer drugs including 5-FU, cisplatin,
oxaliplatin, docetaxel, irinotecan, capecitabine shows a better response than single agent
gemcitabine treatment (171). However the survival rate is still poor and other
combinations of these cytotoxic reagents are needed to treat this disease.
Conceivably miRNA-based therapy of cancer may be achieved by inhibiting
miRNAs with antisense oligonucleotides in cancers that over express the miRNA. 2’-O-
methyl oligoribonucleotides (125, 126) or 2’-O-methyoxyethyl (MOE) phosphorothioate
oligonucleotides (66) are effective, essentially irreversible, inhibitors of mature miRNA
function (125). Various studies have demonstrated that transfection of chemically
51
modified antisense to cancer cell lines produces cell death in vitro (85, 87, 93, 109, 110,
172, 173). In vivo studies of anti-miRs also appear promising. Chemically modified
antisense to the liverspecific miR-122a produced sustained inhibition of the miRNA in
mouse liver in vivo for up to 23 days after a single injection (127). Injection of 2’ MOE
antisense to miR-122 was effective at miR-122 degradation, target gene up regulation and
cholesterol lowering in normal mice (174).
We showed in chapter 2 that aberrant miRNA expression exists in pancreatic
adenocarcinoma (64). By profiling over 200 miRNA precursors in 28 pairs of tissues, we
identified a number of miRNAs with increased expression including miR-21, -221, -301,
-212 and others. There were some miRNAs with decreased expression however the
expression was predominately increased in the cancer. The fold-change in the expression
was quite high (e.g. 5- to 10-fold) in the tumors compared to the normal or adjacent
benign tissue. Our results were corroborated by two additional studies (89, 175).
Szafranska, et al., used cDNA arrays to profile 281 miRNAs in 24 specimens of
pancreatic adenocarcinoma, chronic pancreatitis or normal pancreas (175). Bloomston, et
al., used miRNA arrays to profile the expression of 326 miRNAs in 107 specimens of
pancreatic adenocarcinoma or pancreatitis; to maintain cell heterogeneity, two mm tissue
cores of tumor or benign cells were isolated from the FFPE tissues (89). While some
inconsistencies were apparent between the three pancreatic adenocarcinoma studies (64,
89, 175), there was a fair amount of overlap. For example, both our study (64) and the
study by Bloomston, et al., (89) identified miR-221 as the top differentially expressed
miRNA in pancreatic adenocarcinoma.
52
In this study, we asked the question of whether inhibition of miRNAs that are
overexpressed in pancreatic cancer cell lines with antisense oligos results in cell death.
In addition, can miRNA inhibition sensitize the cells to the antiproliferative effects of
commonly used chemotherapeutic agents such as gemcitabine.
3.2 MATERIAL AND METHODS
3.2.1 Cell lines and culture
The human pancreatic cancer cell lines, Panc-1, Miapaca-2, HS766T, SW1990,
PL45, MPanc-96 and CaPan-1 were purchased from American Type Tissue Collection
(Manassas, VA). Panc-1, MiaPaca-2, HS766T were cultured in DMEM with L-
glutamine and 10% fetal bovine serum. SW1990 was cultured in L-15 medium with 10%
fetal bovine serum. Normal pancreatic ductal epithelial cell line, HPNE (human
pancreatic Nestin-expressing cell) (176), was a gift from Dr. Ouellette (University of
Nebraska Medical center, Omaha, NE). HPNE cells were cultured in Medium D (176).
SW1990 were grown at 37 °C without CO2. All other cells were grown at 37 °C in a
humidified atmosphere with 5% CO2.
3.2.2 miRNA profiling
The expression of 305 mature miRNAs were profiled in PANC-1, MiaPaCa-2,
PL45, MPANC-96, HS766T, CaPan-1, SW1990 and HPNE cells using the TaqMan®
MicroRNA Assays (Applied Biosystems, Foster City, CA) as reported (60). miRNA
53
expression was related to the 18S rRNA internal control and data were calculated using
the comparative CT method.
3.2.3 Antisense oligonucleotides
Anti-miRTM
miRNA inhibitor is purchased from Ambion (Austin, Texas). 2’-O-
methoxyethyl with phosphodiester backbone (2’-O-MOE-PO) and 2’-O-methoxyethyl
with phosphorothioate backbone (2’-O-MOE-PS) antisense oligonucleotides were
provided by Isis Pharmaceuticals (Carlsbad, California). Antisense oligonucleotide
(ASO) is the complementary sequence to the mature miRNA according to miRBase 9.0.
The sequences of the antisense oligonucleotides are shown in Table 3.1.
3.2.4 Antisense oligonucleotide transfection
The cells were plated one day before transfection and antisense were transfected
using lipofectamine 2000 and Opti-MEM medium (Invitrogen, Carlsbad, CA) following
the manufacturer’s protocol. Transfection efficiency was measured using a fluorescently
(DY547) labeled miR-16 oligo and the amount of fluorescence was determined by a
LS55 Luminescence Spectrometer (Perkin Elmer, Boston, MA). The knockdown effect
of the antisense oligonucleotide was measured by quantifying the target mature miRNA
using real-time PCR.
3.2.5 Gemcitabine treatment
Gemcitabine was purchased from Eli Lilly & Co (Indianapolis, IN). Cells were
plated in triplicate at 1200 cells/well in 96 well plates overnight. Gemcitabine was then
54
added at the desired concentrations and incubated for 48 and 96 h. After treatment, cells
were used for cell proliferation assay.
3.2.6 Cell proliferation assay
Cell proliferation assay was performed using the cell proliferation reagent WST-1
(Roche, Mannheim, Germany). Cells were seeded into 96-well plates at 1200 cells/well.
Cells were treated with gemcitabine and/or antisense and incubated for 48 or 96 h. After
incubation, 15 µl of WST-1 was added to cell culture medium and incubated for 4 h.
Sample absorbance was analyzed using a microplate ELISA reader at 490 nm. All
experiments were performed at least in triplicate.
3.2.7 Combination assay
To determine the effects of ASO pretreatment on the gemcitabine potency,
HS766T cells were seeded in 100 mm Petri dishes at the initial density of 2x105 cells and
transfected with various concentrations of control oligonucleotide or ASO to miR-21, -
212, -221 or -301 for 24 h. Cells were then collected by trypsinization and then
transferred to a 96 well plate (3x103 cells/well). After incubating for 24 h to allow cells
to stabilize, gemcitabine was added in a 7 point serial dilution series, 3 replicate plate
columns per treatment. After 72 h of gemcitabine treatment, cell growth inhibition was
determined using WST-1 assay.
For the simultaneous combinatorial experiment, gemcitabine was given with
either ASO to miR-21, -212, -221, or -301 for 96 h. Each ASO was combined with
another ASO, (e.g. ASO to miR-21+ASO to miR-212) in HS766T cells for 96 h. Drugs
55
were combined at equitoxic ratios (i.e. doses were applied in combinations that would
have produced the same cytotoxic effect if the drugs were administered separately to
produce a 50% of growth inhibition, as determined by WST-1 assay). The cytotoxicities
of the two drug combinations were also determined by WST-1 assay using the same
procedure as used for single treatments. Each experiment was performed in triplicate.
3.2.8 Data analysis
ICx defined as the drug concentration required to reduce the absorbance to (100-
X) % of the control in each test was determined using an Emax model (Eq. 1).
( )[ ]
[ ]R
DK
D1R100 viabilityCell %
mm
d
m
+
+−×−= …………… (Eq. 1)
where [D] is the drug concentration, Kd is the concentration of drug that produces a 50%
reduction in absorbance (i.e., IC50), m is a Hill-type coefficient, and R is the residual
unaffected fraction (the resistance fraction).
The nature of interactions between two drugs were determined by using the median effect
analysis (177). Median effect analysis permits the calculation of the combination index
(CI) at various levels of cell kill (Eq. 2). CI was calculated for a cell death range of 20-
80%, i.e., CI20-CI80.
( ) ( ) ( ) ( )
BXAX
BA
BX
B
AX
Ax
)(D)(D
DDα
)(D
D
)(D
DCI ++= ……………… (Eq. 2)
where CIx is the CI for a fixed effect, x [fraction affected (fa)× 100], for a combination of
drug A and drug B, (Dx)A is the concentration of drug A alone giving an effect x, (Dx)B
is the concentration of drug B alone giving an effect x, (D)A is the concentration of drug
56
A in combination A+B giving an effect x, (D)B is the concentration of drug B in
combination A+B giving an effect x, and α is a parameter with value 0 when A and B are
mutually exclusive and 1 when A and B are mutually non-exclusive. A CIx less than 1
was categorized as synergistic and greater than 1 as antagonistic.
3.2.9 Statistical analysis
All data are represented as mean ± S.D. For comparison between two groups,
Student’s t test was used and p < 0.05 is considered as statistically significant.
3.3 RESULTS
3.3.1 miRNA expression in pancreas cell lines
We were initially interested in identifying pancreas cancer cell lines that had
increased expression of many of the top miRNAs that were increased in pancreas cancer
tissues (64). To determine this, the expression of over 300 mature miRNAs was profiled
in 7 pancreas cancer cell lines and in the normal HPNE cells. The results of this profiling
study are presented in Appendix Table B. Previous studies in tumors from pancreatic
cancer patients revealed that a number of miRNAs had increased expression in pancreatic
cancer (64, 89, 175). These include miR-212, -301 and -376a (64); miR-21 and -100 (64,
89); miR-221/-222 and -146 (64, 89, 175); and miR-210 and -223 (89, 175). The
expression values of these top differentially expressed miRNAs in the 7 pancreas cancer
cell lines relative to the normal HPNE cells are shown in Table 3.2. There is a
predominate increase in the expression of many of these miRNAs compared to HPNE.
57
From this analysis we selected five miRNAs for our antisense inhibition studies (miR-21,
-100, -221, -301 and -212). These miRNAs were selected because they had
predominately increased expression in these cell lines and in the pancreas tumors (64).
Also miR-21 and miR-221 have been shown to be increased in a number of solid tumors
and antisense inhibition of both miRNAs reduced clonogenicity (178) or produced cell
death in vitro (87).
3.3.2 Antiproliferative effects of antisense oligonucleotides
The antiproliferative effects of three different chemical modifications to antisense
to miR-221 were studied. These include Anti-miR™ miRNA Inhibitor from Ambion,
Isis 2’-O-MOE-PO (ISIS-PO) and Isis 2’-O-MOE-PS (ISIS-PS). HS766T cells were
transfected with various amounts of antisense for 48 and 96 h. The result showed ISIS-
PS is the most effective antisense (Figure 3.2). For the 96 h treatment of 50 nM, the
percentage survival was 76.3±16.0, 52.9±8.0, and 19.5±2.3 for Ambion, ISIS-PO, and
ISIS-PS, respectively. Therefore, ISIS-PS antisense to miR-21, -100, -212, -221, and -
301 were selected for further studies.
The antiproliferative effects of inhibiting miR-21, -100, -212, -221, and -301 with
ASO was studied for 48 and 96 h of continuous exposure. HPNE (normal pancreatic
ductal epithelial cells) and four human pancreatic cancer cell lines (HS766T, SW1990,
Panc-1 and MiaPACA-2) were transfected with various concentrations of antisense using
lipofectamine 2000. All ASO showed a reduction in cell viability compared to the lipid
control in the human pancreatic cancer cell lines. Inhibition of cell viability with ASO
for 48 and 96 h produced IC50 in the low nM range (Table 3.3). The normal HPNE cells
58
were considerably less sensitive to the effects of some of the ASO (Figure 3.3A, Figure
3.3B and Table 3.3). Inhibition of miR-21 with ASO produced very little
antiproliferative activity in HPNE cells including undetectable IC50 from triplicate
experiments (Table 3.3, Figure 3.3A, and Figure 3.3B). Inhibition of miRNAs with the
other 4 ASOs resulted in slightly greater antiproliferative activity in HPNE cells
compared to ASO-miR-21, although many of these ASO failed to produce a measurable
IC50 or IC70 in the replicate experiments (Table 3.3). Of the 5 ASOs, the IC50 of miR-301
in HPNE most closely approximated that of the pancreas cancer cell lines (Table 3.3).
These results suggest the possibility of selective cytotoxicity to pancreas cancer cells
compared to normal pancreas for some of the ASO.
The possibility exists that the HPNE cells were less sensitive to antisense because
transfection efficiency of the ASO was reduced compared to the pancreas cancer cell
lines. To test this, a DY547 labeled miR-16 oligo was transfected into both HPNE and
HS766T cells using lipofectamine 2000 and the amount of fluorescence was determined
by a spectrometer. The transfection efficiency of the miR-16 oligo was not reduced in
the HPNE cells and in fact was slightly greater than that of the HS766T cells (Figure
3.3C).
Hybridization of the antisense to the mature miRNA should reduce the amount of
freely available miRNA within the cells. To verify this, HS766T cells were transfected
with ASO-miR-221 and then the levels of mature miR-221 within the cells was
determined by quantitative RT-PCR. One hundred nM of ASO-miR-221 reduced the
amount of mature miR-221 by 70% (Figure 3.3D).
59
Experiments were conducted to evaluate any off target effects of the 2’-O-MOE-
PS oligos. A 96 h continuous exposure of up to 100 nM of an irrelevant control oligo did
not reduce cell viability below 20% (Figure 3.3A and 3.4A). Two mismatch control
oligos to ASO-miR-221 were evaluated; one with 3 nts mismatched and another with 6
nts (Table 3.1). There was a trend of reduced cytotoxicity with increasing number of
mismatched nts (Figure 3.4B).
3.3.3 Schedule-dependent combinatorial effects of antisense with gemcitabine
Gemcitabine is one of the agents used to treat pancreatic adenocarcinoma,
however it is only marginally effective. It is possible that miRNA inhibition may
sensitize cancer cells to the effects of commonly-used chemotherapeutic agents such as
gemcitabine. This effect was demonstrated for the combination of ASO-miR-21 and
gemcitabine in cholangioma cell lines (110). At first, we examined whether addition of
gemcitabine to ASO-primed HS766T cells would result in a lower IC50 for gemcitabine.
For this experiment, the cells were pre-treated with antisense for 24 h followed by
gemcitabine for an additional 72 h. We found that sequential addition of gemcitabine to
ASO-miR-21 primed HS766T cells resulted in a reduction in the gemcitabine IC50. The
gemcitabine IC50 was reduced from 5.9 nM (control oligo 50 nM) to 1.2 nM (30 nM of
ASO-miR-21) to 1.0 nM (70 nM of ASO-miR-21), respectively (Figure 3.5A). In
addition, antiproliferative effects of gemcitabine were also enhanced by pretreatment of
ASO-miR-221 (20 nM) and ASO-miR-301 (50 and 70 nM). Effects of low concentration
of gemcitabine ranging from 0.1 to 10 nM were not enhanced by ASO pre-treatment
(data not shown). However, at high effect levels (50, 200, and 500 nM), the
60
antiproliferative effects of gemcitabine were greatly increased in ASO-miR-221 and -301
primed HS766T cells compared to control oligo (Figure 3.5B).
Next, we analyzed the interaction of gemcitabine and ASO on cell survival rate
using the median effect/combination index method after exposing two agents
simultaneously for 96 h. The CIx values calculated for 0.2 ≤ fa ≤ 0.8 (i.e. fraction
affected between 20% and 80%) are shown in Figure 3.5C. All combinations were
examined at a fixed molar ratio based on approximate equitoxic concentrations at the
level of 50% growth inhibition of each agent, i.e. IC50 of drug A: IC50 of drug B. For
each ASO, the IC50 values are shown in Table 3.3. The IC50 value of gemcitabine alone
in HS766T cells was 3.2 ± 0.3 nM for a 96 h exposure. CIx values for the combination
ASO-miR-21+gemcitabine (1:6 molar ratio), ASO-miR-212+gemcitabine (1:2 molar
ratio), ASO-miR-221+gemcitabine (1:2 molar ratio), and ASO-miR-301+gemcitabine
(1:4 molar ratio) varied with fa. CIx values of the above 4 cases were decreased from 2.9,
2.7, 1.8 and 1.3 at fa=0.2 to 0.8, 0.96, 0.68 and 1.2 at fa=0.8, respectively. In the
clinically relevant range of fa ≥ 0.5, ASO-miR-221+gemcitabine combination was
considered to be synergistic. The ASO-miR-21+gemcitabine, ASO-miR-
212+gemcitabine and ASO-miR-301+gemcitabine appeared to be antagonistic. The
antagonistic effects of ASO-miR-21+gemcitabine and ASO-miR-212+gemcitabine were
more evident at lower drug concentration, with CI= 2.9 and 2.7 at fa=0.2, respectively.
3.3.4 Evaluation of synergism in ASO combination
To extend our observations, we evaluated the antiproliferative effects of various
ASO-miR-21, -212, -221, or -301 combined with each other in HS766T cells for 96 h.
61
All combinations were also examined at fixed molar ratio based on approximate
equitoxic concentrations at the level of 50% growth inhibition of each agent. For
ASO+ASO simultaneous combinations, we tested six different combinatorial effects,
which are ASO-miR-21+ASO-miR-212 (1:0.3 molar ratio), ASO-miR-21+ASO-miR-221
(1:0.3 molar ratio), ASO-miR-21+ASO-miR-301 (1:0.7 molar ratio), ASO-miR-
212+ASO-miR-221 (1:1 molar ratio), ASO-miR-212+ASO-miR-301 (1:2 molar ratio),
and ASO-miR-221+ASO-miR-301 (1:2 molar ratio), in HS766T cells. Among them,
ASO-miR-212+ASO-miR-301 showed the greatest synergism with CI value of less than
1.0 (0.55-0.99) for the whole fa range (Figure 3.6), indicating a greater advantage
compared to the other combinations. ASO-miR-221+ASO-miR-301 combination also
showed synergism at fa=0.2, but was found to be antagonistic with CI values ranging
from 0.99 to 2.0 at fa=0.5 and 0.8, respectively (Figure 3.6). When ASO-miR-21 was
combined with ASO-miR-212 or ASO-miR-221, slight synergistic effects were shown:
the CI80 was 0.80 for the ASO-miR-21+ASO-miR-212 and CI20 was 0.68 for the ASO-
miR-21+ASOmiR-221 (data not shown). However, the CI20 and CI80 were 1.78 and 1.79
for ASOmiR-21+ASO-miR-212 and ASO-miR-21+ASO-miR-221, respectively,
indicating antagonistic interactions. ASO-miR-21 was also treated with ASO-miR-301
and showed antagonistic interactions (CI values > 1) for the whole fa range. On the other
hand, only antagonistic interactions were induced by ASO-miR-212+ASO-miR-221 with
CI values increasing from 1.6 to 2.5 by escalating concentrations of each ASO (data not
shown).
62
3.4 DISCUSSION
The results of our current study demonstrate that inhibition of 5 miRNAs that are
over expressed in pancreas cancer produced cell death in vitro. Low nM concentrations
of ASO reduced cell proliferation in pancreatic cancer cell lines. ASO inhibition of
miRNA in the normal HPNE cells had less pronounced effects on cell proliferation
compared to the pancreas cancer cell lines (Figures 3.3A and 3.3B and Table 3.3).
Certain 2’-O-MOE-PS ASOs that were not complementary to any known human gene
were observed to substantially effect cell proliferation at concentrations of 100-300 nM in
vitro (179). Phosphorothioates in general have been shown to produce toxicity (reviewed
in (129)). Therefore it is possible that a portion of the cytotoxicity from the 2’-O-MOE-
PS was due to off target effects. Exposing cancer cell lines to an irrelevant control 2’-O-
MOE-PS oligo produced modest effects on cell proliferation (Figures 3.3A and Figure
3.4A). Likewise, altering the sequence of ASO-miR-221 reduced its antiproliferative
activity (Figure 3.4B).
Standard palliative gemcitabine treatment showed only a 6 month median survival.
Although many trials have been attempted to improve upon the overall survival of
gemcitabine based combination chemotherapy, only the gemcitabine/Erlotinib
combination showed a slight increase in the overall survival, but survival times
associated with combinations of gemcitabine/fluorouracil, gemcitabine/irinotecan,
gemcitabine/cisplatin, and gemcitabine/oxaliplatin appear to be either equivalent or only
marginally superior to those associated with single-agent gemcitabine (180). A phase III
study showed that a 4-drug PEFG (cisplatin, epirubicin, 5-fluorouracil (5-FU),
63
gemcitabine) regimen obtained a response rate of 38-51% and a 1-year overall survival of
38.5-39%, without compromising quality of life, which was significantly better than
single-agent gemcitabine (180). However, there are still no guidelines or
recommendations for selecting treatment for pancreatic cancer patients, and both
acquired drug resistance and considerable systemic toxicities are major reasons for the
limited advances made in cancer chemotherapy. From this point of view, our data
showing the antiproliferative effects in various pancreatic tumor cells, reduced sensitivity
of antisense toward normal HPNE cells and enhancement of gemcitabine antiproliferative
effects with ASO-miR-21, -221 and -301 and a synergistic effect between gemcitabine
and ASO-miR-221 (Figure 3.5) suggests that ASO can be used as future gemcitabine-
based combination regimen. Moreover, our results showing the greatest synergism of the
ASOmiR-212+ASO-miR-301 combination (Figure 3.6) also imply, at least in part, that
we can expect greater effects than gemcitabine-based combinations. Overall,
experiments described herein strongly support the potential for the miRNA-based
targeted therapy of pancreatic cancer using antisense oligonucleotide and the possible
target molecules and more detail mechanisms that are responsible for anti-proliferation
and sensitization effect should be further investigated.
3.5 ACKNOWLEDGEMENTS
Supported by an NIH grant CA107435 to T.D.S. and a grand from the National
Pancreas Foundation. We thank Dr. Caifu Chen at Applied Biosystems for his assistance
with the TaqMan looped primer assays and Christine Esau at ISIS Pharmaceuticals for
64
her assistance with the Antisense oligonucleotides. We also thank Jong-Kook Park with
experiments assistance.
65
Figure 3.1. Structures of Gemcitabine and Deoxycytidine.
66
ASO221(48hr)
lipo 20 50 250 500 lipo 20 50 250 500 lipo 20 50 250 500
Su
rviv
al(%
)
0
20
40
60
80
100
120
Ambion-221
ISIS(PO)-221
ISIS(PS)-221
ASO221(96hr)
lipo 20 50 250 500 lipo 20 50 250 500 lipo 20 50 250 500
0
20
40
60
80
100
120
140
Ambion-221(96)
ISIS(PO)-221-96
ISIS(PS)-221-96
Su
rviv
al(
%)
Figure 3.2. Comparison of three different antisense oligonucleotides.
WST-1 assay in pancreatic cancer (HS766T) cells show the relative cytotoxicity of the
antisense to miR-221. Ambion anti-miR was compared to Isis 2’ MOE-PO and Isis 2’
MOE-PS. Cells were exposed continuously for 48 h (top) or 96 h (bottom). Lipo, lipid
only control. Cells were transfected using lipofectamine 2000.
67
Figure 3.3. The effect of antisense oligonucleotide and control oligonucleotide in
pancreatic cell lines.
(A) Representative dose-response curve of ASO-miR-21 and ASO-miR-221 and control
oligonucleotide in HS766T and HPNE. Cells were transfected with oligonucleotides and
incubated continuously for 96 h. Filled symbols are HS766T, open symbols are HPNE,
circles are ASO-miR-21, triangles are ASO-miR-221, and squares are control
oligonucleotide. (B) Antiproliferative effects of ASO-miR-21 and ASO-miR-221 (50 nM,
96 h) in 5 pancreatic cell lines. (C) Cellular uptake of fluorescently labeled miR-16
oligonucleotide in HS766T and HPNE cells. (D) The knockdown effect of ASO-miR-
221 in HS766T cells. The expression levels of the target microRNA were assessed by
real-time RT-PCR 24 hours after transfection. Data presented in (B) and (D) are the
mean of 3 independent experiments (* P < 0.05; ** P < 0.01).
68
69
Figure 3.4. Effect of control oligonucleotides on cell proliferation.
(A) Transfection of control oligonucleotide in HS766T cells for 96 h; data are compared
to lipofectamine control. Mean data from triplicate transfections. (B) Comparison of
wild type ASO-miR-221, 3 nt and 6 nt mismatch control oligonucleotides on the
proliferation of HS766T cells; 96 h exposure. Mean data of 3 independent experiments.
70
Figure 3.5. ASO pretreatment sensitized the antiproliferative effects of gemcitabine.
Antisense to miRNAs are transfected in HS766T cells 24 h before gemcitabine treatment
and antiproliferative effects of gemcitabine are measured after 72 h treatment by WST-1
assay. ASO sensitized the gemcitabine effect showing that dose-response curve is shifted
to the left. (A) Representative dose-response curve of gemcitabine after ASO-miR-21(30
nM ●, 70 nM ■,) and control oligonucleotide pretreatment (50 nM, ○). (B) ASO-miR-
221 and ASO-miR-301 sensitize the gemcitabine effect at the high concentrations. (C)
Combination index (CIx) versus fraction affected (fa) plots in HS766T cells for
ASOmiR-21+gemcitabine (), ASO-miR-212+gemcitabine ( ), ASO-miR-
221+gemcitabine (····), and ASO-miR-301+gemcitabine (········).
71
72
Figure 3.6. Combination index (CIx) versus fraction affected (fa) plots in HS766T
cells for ASO-miR-212 + ASO-miR-301 ( ) and ASO-miR-221 + ASO-miR-301
(). CI < 1 and CI > 1 indicate synergism and antagonism, respectively. CIx was
calculated using Emax model parameters obtained from the WST-1 assay data.
73
Isis number
miRNA Sequence
(5’ to 3’)
327917 miR-21 TCAACATCAGTCTGATAAGCTA
327957 miR-100 CACAAGTTCGGATCTACGGGTT
327891 miR-212 GGCCGTGACTGGAGACTGTTA
346908 miR-301 ACGTGGATTTTCCTCTATGAT
327919 miR-221 GAAACCCAGCAGACAATGTAGCT
394088 miR-221
3 nt mismatch GAATCCCAGCAGAGAATGTTGCT
394091 miR-221
6 nt mismatch GAATCACACCAGAGAAAGTTGCT
386666 Irrelevant control ACATACTCCTTTCTCAGAGTCCA
Table 3.1. Sequence and chemical modifications to antisense oligonucleotides.
Each of the antisense oligonucleotides is modified with uniform MOE
w/phosphorothioate backbone [AGTmC]. Each has a 5’ end cap of hydrogen and 3’ end
cap of hydroxyl.
74
Table 3.2. miRNA expression, pancreas cancer cell lines normalized to normal pancreas cell (HPNE).
miRNA PANC-1 MIAPACA-2 PL45 MPANC-96 HS766T CaPan-1 Sw1990
miR-21 2.9 2.8 3.1 5.2 9.1 7.7 2.2
miR-221 0.6 2.5 1.2 2.0 1.9 0.4 1.7
miR-100 1.3 0.0 0.2 0.2 0.8 0.0 0.3
miR-212 3.3 2.3 2.1 1.5 3.0 0.6 1.0
miR-301 21.2 52.9 11.6 15.3 86.8 5.1 7.7
miR-376a 3.0 0.0 0.0 0.0 0.0 0.0 0.0
miR-155 3.0 0.1 2.0 1.9 14.7 0.5 1.5
miR-210 1.8 1.2 1.2 4.1 0.9 4.1 1.9
miR-223 1.2 1.8 0.0 3.3 0.2 1.3 0.7
miR-146 59.1 6.6 2.4 35.7 7.0 99.9 3.7
miR-222 0.6 1.9 1.4 0.9 1.7 0.4 1.3
miR-24 1.1 1.1 0.8 1.6 0.9 1.8 2.2
Red > 2-fold increase; Blue > 2-fold decrease
75
Table 3.3. Antiproliferative activity parameters of ASO against miR-21, -100, -212, -
221 and -301 in human pancreatic cell lines. The dose-response curves of these agents were analyzed using the Emax model (Eq. 1).
76
Cell lines Treatment
duration Parameter Anti-miR-21 Anti-miR-100 Anti-miR-212 Anti-miR-221 Anti-miR-301
IC50a 48.3±4.4 57.3±16.7 58.7±22.0 32.4±2.3 57.0±14.0
IC70 a 98.4±10.9 136.7±68.9 113.9±29.1 99.4±29.5 106.6±30.2
48 h
Rc 13.8±7.6 8.9±15.5 0 18.3±15.9 0
IC50 a 28.4±8.9 19.2±3.7 12.3±3.9 12.1±4.3 24.0±1.9
IC70 a 44.0±12.8 31.4±3.7 22.4±4.3 21.0±5.2 39.6±1.2
HS766T
96 h
Rc 0 2.8±4.9 0 3.6±6.2 0
IC50 a ND
b ND
b ND
b ND
b 43.3±12.4
IC70 a ND
b ND
b ND
b ND
b 138.1±59.3
48 h
Rc 74.0±14.4 62.8±18.7 52.7±6.6 57.9±1.3 22.6±1.3
IC50 a 21.4±7.9 16.2±12.1 18.8±6.5 10.1±5.7 14.9±4.0
IC70 a 42.6±8.7 36.6±17.8 37.4±12.7 16.1±9.6 26.8±4.9
SW1990
96 h
Rc 13.8±7.1 1.1±1.9 11.1±6.4 15.1±5.7 2.7±2.5
IC50 a 22.3±18.6 21.2±11.5 71.6±43.7 24.9±16.1 41.9±9.6
IC70 a 43.6±21.3 48.0±13.8 191.8±162.9 51.5±12.5 62.8±17.2
48 h
Rc 3.5±6.1 3.3±5.6 0 <1.0 8.2±3.4
IC50 a 15.0±7.8 9.3±4.3 12.2±4.1 2.5±1.7 20.4±1.6
IC70 a 24.8±8.9 17.4±8.1 16.7±7.1 3.6±2.6 26.6±10.7
MiaPACA-2
96 h
Rc 6.1±8.9 5.5±5.2 <1.0 10.3±9.8 <1.0
IC50 a 66.1±32.8 ND
b ND
b 64.3±24.4 89.4±18.9
IC70 a ND
b ND
b ND
b ND
b ND
b
48 h
Rc 39.3±7.7 63.0±6.2 66.2±13.8 39.4±6.9 47.7±5.2
IC50 a 37.9±3.5 50.5±10.3 55.3±25.6 10.1±2.9 49.9±21.8
IC70 a ND
b ND
b 69.9±14.7 ND
b 81.6±36.9
Panc-1
96 h
Rc 34.6±1.4 37.7±4.5 16.5±10.8 31.9±2.3 23.4±11.5
IC50 a ND
b ND
b 121.8± ND
e 1,479±1,842 155.8±49.9
IC70 a ND
b ND
b 217.1±ND
e 479.7± ND
e 285.6± ND
e
48 h
Rc 95.6±11.7 86.8±18.0 63.6±7.90 70.7±8.98 55.5±6.38
IC50 a ND
b 87.1±17.6 66.3±10.5 44.2±17.6 56.5±10.5
IC70 a ND
b 214±ND
e 120.9±10.8 88.6±7.60 87.1±6.0
HPNE
96 h
Rc 73.2±26.3 62.0±25.3 34.0±5.15 31.0±6.33 23.9±1.99
77
CHAPTER 4
ANTISENSE INHIBITION OF microRNA ARRESTS CELL CYCLE, INDUCES
APOPTOSIS AND IDENTIFIES THE TARGET MOLECULES IN PANCREATIC
CANCER
4.1 INTRODUCTION
A growing body of data implicates altered miRNA expression in cancer. The
initial study to establish a link between miRNA and cancer was from the Croce
laboratory. Numerous profiling studies have shown that miRNAs are differentially
expressed in a number of human cancers including thyroid (90, 181), breast (99, 182),
lung (98), pancreatic adenocarcinoma (64, 89, 175), glioblastoma (87, 100),
hepatocellular (81, 82), prostate (183), colorectal (184, 185), leukemia (75, 153) and
lymphoma (101). A polycistron encoding miRNAs miR-17, -18, -19a, -19b-1 and -92-1 is
amplified in human B-cell lymphomas and forced expression of the polycistron along
with c-myc was tumorigenic, suggesting that this group of miRNAs may function as
oncogenes (94). Clear evidence exists that miRNAs are involved in cancer.
What is less clear is the mechanism(s) by which altered miRNA expression
contributes to the development or maintenance of cancer. To understand the function and
78
mechanistic pathway of miRNA, the miRNA’s target gene must be discovered and
properly validated. The miRNA’s target gene may be identified from a number of freely
available data bases. However, these programs have a high percentage of false positive
results. An issue with these prediction algorithms is that since the miRNA does not need
to bind with 100% complimentarity (except the 5’ seed), several hundreds of potential
targets may be predicted for a given miRNA. Using miR-21 as an example, TargetScan,
miRanda and PicTar predict 192, 1000 and 175 targets, respectively. Different prediction
programs follow their own criteria so each program predicts different targets. Therefore,
it is important to narrow down the list of target candidates using several programs and to
properly validate the predicted target.
The target site accessibility can be considered as an important criterion in target
prediction because mRNA secondary structure may inhibit the target gene and miRNA
binding. Accessibility is estimated by the free energy of the flanking 70 nts at the 3’ and
5’ ends of the target site in the 3’ UTR (70). Once a target candidate looks promising, the
miRNA and mRNA binding should be validated using a reporter assay. The 3’ UTR
sequence of the target candidate gene is cloned downstream of a reporter gene such as
luciferase or green fluorescent protein (GFP). This cloned plasmid is transfected with
miRNA mimic or negative control into cells and the expression of reporter gene is
measured in 24-48 h after transfection. For controls, a mutated seed sequence, reversed
sequence of 3’ UTR, or vector only may be used. Another way to check miRNA/mRNA
binding is to precipitate Ago complex and compare the sequences of the 3’ UTR and
miRNA to identify functional miRNA targets in vivo (186).
79
After the binding assay, the protein level of the target gene should be validated by
western blot to see if gene knockdown affects the actual protein function. If an antibody
for target protein is not available, other validation such as ligand binding assay should be
performed (187). Target protein expression may be validated using gain of function or
loss of function studies. If endogenous miRNA is highly expressed, miRNA expression
can be blocked using antisense oligonucleotides resulting in an increase in the target
protein level. However, if the target gene expression is high and the miRNA expression
is not high enough to see a change of protein expression, a miRNA mimic may be added
and target protein should decrease. In addition, genes down stream of the miRNA’s
target gene can be checked to see biological function of target gene. For example, the
Patel laboratory identified PTEN as a target gene of miR-21 (110) and they showed that
inhibition of miR-21 in human hepatocellular cancer increased PTEN level and decreased
tumor cell proliferation, migration, and invasion (86). They also demonstrated that focal
adhesion kinase phosphorylation and matrix metalloproteases 2 and 9, which are
downstream of PTEN, are involved in cell migration and invasion (86).
In our previous study, antisense oligonucleotides to miRNA-21, -212, -221 and
301 showed anti-proliferation effect at low nM concentration and sensitized the effect of
gemcitabine in a schedule-dependent way. Here, we investigated the effects of antisense
oligonucleotides on the cell cycle and apoptosis. We validated that PTEN and RECK are
target molecules of miR-21 and that p27(kip1) is the target of miR-221 in pancreatic
cancer. We also used a reporter assay to identify new target genes for miR-212 which is
FOXO3a.
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4.2 MATERIAL AND METHODS
4.2.1 Cell lines and culture
The human pancreatic cancer cell line HS766T was purchased from American
Type Tissue Collection (Manassas, VA). HS766T was cultured in DMEM with L-
glutamine and 10% fetal bovine serum, and it was grown at 37°C in a humidified
atmosphere with 5% CO2.
4.2.2 Antisense Oligonucleotides and Pre-miR™ miRNA Precursor
2’-O-methoxyethyl with phosphorothioate backbone (2’-O-MOE-PS) antisense
oligonucleotides was provided by Isis Pharmaceuticals (Carlsbad, California). Antisense
oligonucleotides sequences are presented in Table 3.1. Pre-miR™ miRNA Precursor
Molecule was purchased from Ambion (Austin, Texas).
4.2.3 Antisense oligonucleotide and Pre-miR™ miRNA Precursor transfection
HS766T cells (2x105 cells in 100 mm dishes) were plated one day before
transfection and antisense or pre-miR were transfected using lipofectamine 2000 and
Opti-MEM medium (Invitrogen, Carlsbad, CA) following the manufacturer’s protocol.
Cells were incubated for 24 h, 48 h or 72 h depending on experiments.
4.2.4 Cell cycle analysis
To determine cell cycle distribution, cells were collected after trypsinization into a
single cell suspension, fixed with cold ethanol at 4 °C for 1 h prior to being stored at -20
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°C until required for analysis. Upon analysis, fixed cells were washed and resuspended
in 1 ml of PBS containing 50 µg/ml of RNase A and 50 µg/ml of ethidium bromide.
After incubating for 20 min at 37 °C, cells were analyzed for DNA content by flow
cytometry (FACS Caliber, Becton Dickinson Immunocytometry Systems, San Jose, CA).
For each sample, 10,000 events were acquired and cell cycle distributions were
determined using cell cycle analysis software (Modfit®
, Verity, Topsham, ME).
4.2.5 Apoptosis analysis
Annexin V–FITC/propidium iodide (PI) staining was done to identify the
induction of apoptosis in HS766T cells treated with ASO. HS766T cells were plated in
100 mm Petri dishes at 2x105 cells per dish and cultured overnight. Twenty or 48 h after
ASO treatment, cells were collected and washed with PBS. Cells were stained with
Alexa Fluor® 488 annexin V and PI using Vybrant® Apoptosis Assay Kit #2 (Molecular
Probes, Eugene, OR) according to the manufacturer's protocol. Cells were analyzed
using flow cytometry (FACS Caliber, Becton Dickinson) and CellQuest software (Becton
Dickinson). Gating was selected using untreated cells to separate the majority of
doublenegative cells from minor populations of PI or Annexin V-FITC–positive cells.
Cells that were FITC–/PI– were considered viable, FITC+/PI– cells were considered
early apoptotic, and FITC+/PI+ cells were considered nonviable.
4.2.6 Real-time PCR
Total RNA was isolated from cells using Trizol (Invitrogen, Carslbad, CA) per
the manufacturer’s instructions. RNA was treated with DNase I and 1 µg of RNA was
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reverse transcribed using random primers.
The expression of FOXO3a was measured using a SYBR green real-time
quantitative PCR assay and normalized to 18S rRNA. Relative gene expression was
calculated using 2-(C
TFOXO3a – C
T18S rRNA) . PCR primers for FOXO3a were followings;
sense primer, 5’-TTGCGTGCCCTACTTCAA-3’ and antisense primer, 5’-
GCATGAATCGACTATGCAGTG-3’.
4.2.7 Plasmid constructs
The 3’ UTR of the human FOXO3a (FKHRL1) was amplified using the following
sense (5’-ATGCCTCGAGATCTGCTCTCAGCAAGTGGA-3’) and antisense primer
(5’-ATTAGCGGCCGCTGGAGAGCTGAGCTGATTTCT-3’) containing enzyme
restriction site (XhoI and NotI) at the both ends. Amplified PCR product was cloned at
downstream of the Renilla luciferase stop codon in psiCHECKTM
-2 vector (Promega,
Madison, WI). Renilla luciferase is a primary reporter gene in psiCHECKTM
-2 vector
and this vector also contains a secondary Firefly luciferase reporter cassette, which was
used to normalize the Renilla luciferase signal. Constructed plasmid was verified by
DNA sequencing.
4.2.8 Luciferase assay
HS766T cells (0.5x105
cells) were plated in 24 well plates one day before
transfection. Plasmid construct was transfected with pre-miR-negative control or pre-
miR-212 using lipofectamine 2000 and Opti-MEM medium (Invitrogen, Carlsbad, CA).
Cells were harvested 24h later and Renilla and Firefly luciferase activity were measured
83
using the Dual Luciferase Assay (Promega, Madison, WI) according to the company’s
protocol.
4.2.9 Western blotting
HS766T cells (2x105 in 100 mm dish) were treated with an IC50 dose of ASO to
miR-21, -221 and -212 for 24, 48, and 72 h or with a 30 nM or 60 nM of pre-miR-212.
Cells were washed in ice-cold PBS and lysed in RIPA buffer containing protease
inhibitor. Protein concentration was measured using the BCATM
protein assay kit (Pierce,
Rockford, IL). Thirty µg of total protein extract was separated on a 10% SDS-PAGE gel.
Blotting was performed with the following antibodies: PTEN (Cell signaling, Danvers,
MA), RECK (Santa Cruz Biotechnology, Santa Cruz, CA), p27 (kip1)
(Abcam,
Cambridge, MA) and FOXO3a (Upstate, Temecula, CA). β-actin (Cell signaling, Danver,
MA) was used as a loading control. Secondary HRP antibody was detected using ECLTM
Western Blotting Analysis System (Amersham Biosciences, Piscataway, NJ).
4.3. RESULTS
4.3.1 Effects of miRNA inhibition on the cell cycle
The effects of inhibiting miR-21, -100, -221, -301 and -212 on the cell cycle were
studied in HS766T cells. A 48 h exposure of 20 nM of control oligo produced 57.1% of
G1, 12.4% of G2, 30.5% of S phase and a slight increase in the sub-G1 phase compared
to the lipid control (Figure 4.1A), however it produced no net effect on the cell cycle. An
increase in the sub-G1 fraction suggests the presence of apoptosis. Even at a higher
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concentration of 40 nM (48 h exposure), the control oligo showed almost the same cell
cycle distribution as the lipid control (data not shown). miR-21 inhibition resulted in
62.2% of G1, 15.9% of G2, and 21.9% of S phase at IC50 (48 h exposure) and the G1
phase was increased to 65.2% at the IC70 (48 h exposure). This demonstrates that miR-
21 inhibition produced a G1 phase arrest as the amount of cells in the G1 phase increased
by about 10% and the amount of cells in the S phase decreased by about 10% (Figure
4.1B). Inhibition of miR-301 increased the sub-G1 fraction to 42.5% at an IC50 dose
(Figure 4.1C). Inhibition of miR-212 produced a 19.6% of G2 at IC50 and the G2 phase
was increased to 30.3% at an IC70 dose (Figure 4.1D). Thus ASO-miR-212 produced a
G2 phase arrest. Conversely, inhibiting miR-221 did not show any noticeable changes in
the cell cycle at the IC50 by 48 h (data not shown) and or an IC70 treatment (data not
shown). Inhibition of miR-100 also didn’t show any prominent changes at IC50 (48 h
exposure, data not shown).
4.3.2 Effect of miRNA inhibition on apoptosis
Since the results of the cell cycle analysis demonstrated that inhibition of some
miRNAs increased the sub-G1 fraction and antisense to miR-21 has been shown to
induce apoptosis (87), we wanted to examine the effects of miR-21, -221, -212 and -301
inhibition on apoptosis. This was done to determine whether the antiproliferative effects
of miRNA inhibition were due to apoptosis induction. Phosphatidylserine externalization
is a parameter of ongoing apoptosis, which can be measured by Annexin V staining. The
results of Annexin V staining showed that early apoptotic cells (Annexin V positive cells)
were increased in a time-dependent manner in HS766T cells (Figure 4.2). Only 4.9±1.2
85
% and 11.4±2.9% of cells were annexin V positive after 24 and 48 h incubation of 50 nM
of control oligo, respectively. However, HS766T cells transfected with ASO-miR-21, -
212, -221 or -301 induced 19.9%, 6.0%, 10.2% and 15.8% of annexin V positive cells
after 24 h, respectively. Furthermore, the transfection of each ASO for 48 h dramatically
increased the apoptotic cells compared to control oligo. The percentage of cells staining
positive with Annexin V was 58.2%, 28.8%, 36.6%, and 53.1% for ASO-miR-21, -212, -
221, and -301, respectively (Figure 4.2). Relatively low concentrations (25 nM) of ASO-
miR-21 and -301 also showed 21.0±1.5 and 25.4±1.3% of Annexin V positive cells after
48 h incubation (data not shown). In addition, these data showed that ASO-miR-21 and -
301 induce a relatively higher amount of apoptotic cells compared to ASO-miR-212 and -
221.
4.3.3 PTEN, RECK and p27(kip1) validation as target of miR-21 and miR-221
miRNAs exert their activity by binding to conserved sequence within the 3’ UTR
of protein coding mRNAs. miRNA target discovery and validation is an active area of
research and the targets to few mammalian miRNAs have been discovered. Some of the
mRNA targets to miR-21 and miR-221 have been recently discovered. miR-21 has been
shown to target the tumor suppressor PTEN (110). Three groups working independently
demonstrated that miR-221 targets the tumor suppressor p27(kip1) (178, 180, 188). The
tumor suppressor RECK (reversion-inducing cysteine-rich protein with Kazal motifs) is
predicted to be a target of miR-21 (107). In order to validate that the ASO to miR-21 and
miR-221 is working through a miRNA effect (i.e. at the level of translation), we
performed Western blots to detect these tumor suppressors in HS766T cells treated with
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control oligo or antisense oligonucleotide for various times. The amount of p27(kip1)
protein increased over the 72 h period of ASO-miR-221 treatment (Figure 4.4B). This
demonstrates that as miR-221 is inhibited by antisense, less miRNA is available to
negatively regulate the p27(kip1) mRNA. ASO-miR-21 increased both PTEN and RECK
levels at 24, 48 and 72 h of exposure (Figure 4.4A).
4.3.4 FOXO3a as a new target of miR-212
FOXO3a is one of the mammalian forkhead members of the class O (FOXO)
transcription factors and is involved in cell cycle arrest and apoptosis (reviewed in (189)).
Since ASO-miR-212 showed cell cycle arrest at G2/M phase and also induced apoptosis,
we wanted to identify a possible target gene of miR-212 that explains the mechanism of
cell cycle arrest and apoptosis. First, target candidates of miR-212 were investigated
using several target prediction programs such as TargetScan, miRanda and PicTar.
Among numerous predicted target genes, FOXO3a was one of promising candidates
because all three programs predicted FOXO3a as a target of miR-212 and it is a gene that
regulates both cell cycle and apoptosis. FOXO3a contains a putative miR-212 binding
site in its’ 3’ UTR. The potential base pairing between FOXO3a and miR-212 is shown
in Figure 4.4A. The binding site is highly conserved in several species (Figure 4.4A).
It was reported that the secondary structure of the 3’ UTR of the target gene may
prevent miRNA from binding and that miRNA accessibility may be a critical
characteristic of target binding (70). To further confirm that FOXO3a may be a target
gene of miR-212, we calculated the free energy of the ~ 70 nt flanking the 5’ and 3’
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regions of the predicted binding site using mFold (190). The values are -7.8 and -12.8
kcal/mol, respectively, showing a favorable binding.
The expression of mRNA level of FOXO3a was quantified using a real-time PCR
assay after antisense oligonucleotide (ASO) transfection. HS766T cells were treated with
either control oligonucleotide (20 nM) or ASO-miR-212 (20 nM) for 24 h and 48 h. The
data demonstrated that the mRNA level was significantly increased in the cells that were
treated with ASO-miR-212 for 48 h compared to untreated or control oligonucleotide
treated cells (Figure 4.4 B).
To verify whether miR-212 interacts with the 3’ UTR of the FOXO3a, FOXO3a
3’ UTR was cloned downstream of the Renilla luciferase translational stop codon. This
plasmid construct was transfected into HS766T cells with either pre-miR-negative control
or pre-miR-212 oligonucleotides. Cells were incubated for 24 h and Renilla luciferase
activity was normalized with Firefly luciferase activity. As shown in Figure 4.5A, the
luciferase activity in cells treated with pre-miR-212 was significantly reduced by
approximately 50% compared to the cells with pre-miR-negative treatment, indicating
that miR-212 binds to the 3’ UTR of FOXO3a.
miRNA binds to the 3’ UTR of the target gene and represses the target gene
translation (11). To determine whether miR-212 regulates FOXO3a expression through
translational repression, we performed western blotting with anti-FOXO3a antibody
(Figure 4.5 B and 4.5 C). We treated the cells with ASO-mir-212 in various time points
and protein is up-regulated at 48 h and 72 h (Figure 4.5 B). In addition, cells were treated
with pre-miR-negative and pre-miR-212 oligonucleotide at 30 nM and 60 nM and protein
88
level of FOXO3a was reduced (Figure 4.5 C). The results that we showed here
demonstrate that FOXO3a is a target gene of miR-212.
4.4 DISCUSSION
Consistent with previous reports, antisense to miR-21 resulted in a concentration
dependent G1 phase arrest and induction of apoptosis in the pancreatic cancer cell line
HS766T (Figure 4.1 and 4.2). miR-21 is highly expressed in human glioblastoma tumor
tissues and knockdown of miR-21 induced activation of caspases (87). Apoptosis
induced by ASO-miR-21 was due to downregulation of Bcl-2 in the MCF-7 breast cancer
cell line (85). In addition, the tumor suppressor PTEN that coordinates G1 cell cycle
arrest is a validated target of miR-21 (110). In HS766T pancreatic tumor cells, treatment
of ASO-miR-21 also increased PTEN protein expression (Figure 4.3A) and induced a G1
cell cycle arrest (Figures 4.1B). We also observed that miR-212, -221 and -301 regulates
the cell cycle and/or apoptosis in pancreatic tumor cells.
ASO-miR-21 also increased RECK protein levels in HS766T cells over the 72
hours of exposure. RECK is a membrane bound protein that inhibits matrix
metalloproteinases and angiogenesis (191). Increased expression of miR-21 in pancreatic
adenocarcinoma (64, 89), may likely suppress RECK protein levels. In fact, pancreatic
adenocarcinomas with positive RECK staining were less invasive compared to RECK
negative tumors and patients who had tumors with high RECK expression showed
significantly better prognosis than those with RECK-negative tumors (192).
89
Previous studies demonstrated that p27(kip1), which plays a key role in the
growth arrest at the G1/S transition, is one of the targets of miRNA-221 (178, 180, 188).
Visone, et al., showed that treatment of TPC-1 cells with miRNA-221 mimetic increased
the transition from the G1 to S phase and reduced p27(kip1) protein (180). Also, ectopic
expression of miR-221 and -222 enhanced the progression to the S phase of cell cycle,
therefore, increasing the growth of LNCaP cells (180). Of major importance in our
previous data is that the overall antiproliferative effects of ASO-miR-221 were
significant in pancreatic tumor cells (Figures 3.3). However, contrary to our expectations,
there was no cell cycle change after transfection of anti-miR-221 in HS766T cells even
though transfection of ASO-miR-221 reduced the amount of miR-221 (Figure 3.3D) and
the level of p27(kip1) protein was increased after 48 h of treatment (Figure 4.3B). This
result may be due to the possibility that apoptosis induced by ASO-miR-221 was cell
cycle independent and apoptotic cells mainly emerged from the G1 phase and therefore
resulted in no net changes in the cell cycle pattern. Also, bioinformatic analyses suggest
a great number of predicted targets of miRNA-221. If inhibition of miR-221 by antisense
may increase some proteins impairing p27(kip1) nuclear import in pancreatic cancer cells
in our experimental conditions, cell cycle arrest effects could be overcome.
ASO-miR-212 significantly blocked G2/M phase with abrogation of G1 phase
and ASO-miR-301 induced apoptosis without arresting the cell cycle (Figure 4.1 and 4.2).
These observations made us further investigate and elucidate the miRNA target genes
related with cell cycle and apoptosis mechanisms in pancreatic tumor cells. In this study,
we identified FOXO3a as a target gene of miR-212.
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Forkhead box O transcription factors are the mammalian orthologs of C.elegans
transcription factor DAF-16 (193, 194) and FOXO3a (FKHRL1) is one of the FOXO
family members (195). FOXO3a is involved in apoptosis, cell cycle regulation, oxidative
stress and longevity (189, 196, 197). It is reported that FOXO3a functioned as a tumor
suppressive gene causing cell death in several cancers (198-200). For example, FOXO3a
inhibits estrogen-dependent breast cancer cell growth and tumorigenesis (198) and the
transcription factor RUNX3, which mediates apoptosis and cell growth inhibition in
gastric epithelial cells, physically interacts with the FOXO3a and induces apoptosis,
activating Bim (199). Also, Chen et al. (200) demonstrated that the accumulation of
FOXO3a increased radiosensitivity and patients’ survival was enhanced. FOXO3a also
regulates cell cycle. It arrests the G1/S phase during the cell cycle either in a p27(kip1)-
dependent (201) or p27(kip1)-independent (202, 203) manner. In addition, FOXO3a
induces G2/M phase delay or arrest through the GADD45a (growth arrest and DNA
damage inducible alpha protein) or cyclin G2 (189, 204). FOXO3a directly binds and
activates the promoter of GADD45a (189, 204). Tran et al. also used GADD45a-
deficient fibroblasts demonstrating that G2/M arrest by FOXO3a at least partly requires
GADD45a (204). In contrast, one paper showed that GADD45a is overexpressed in
pancreatic adenocarcinoma and it enhances cell proliferation in pancreatic cancer (205).
Yamasawa et al. reported that GADD45a is expressed in 54.2% of resectable invasive
ductal carcinomas (IDCs) of the pancreas and 13.6% of IDC patients showed a point
mutation in the GADD45a gene (206). These two results are contradictory with other
reports and our results. However, they did not explain the further mechanism of
91
overexpressed GADD45a and the expression of GADD45a in those two cases might be
affected by other upstream signaling or other miRNAs.
To date, no one has identified a target gene of miR-212. Here, we demonstrated,
for the first time, that ASO-miR-212 induced apoptosis and caused G2/M arrest during
the cell cycle (Figure 4.1 and 4.2D), regulating the FOXO3a expression at the RNA and
protein level (Figure 4.4 and 4.5). We are currently investigating the downstream gene
that might be affected by the FOXO3a to better explain the mechanism of apoptosis and
cell cycle. We expect that GADD45a or cyclin G2 are possible downstream genes to
explain G2/M arrest and Bim is a candidate for apoptotsis. Moreover, the G2/M arrest
was more significant in Panc-1 cells compared to HS766T after ASO-miR-212 treatment
(unpublished data). Therefore, the correlation of GADD45a level and the effect of G2/M
arrest between Panc-1 and HS766T will be studied and these results may explain why
miR-212 causes a cell cycle arrest and apoptosis in pancreatic cancer.
4.5 ACKNOWLEDGEMENTS
Supported by an NIH grant CA107435 to T.D.S. and a grand from the National
Pancreas Foundation. We thank Christine Esau at ISIS Pharmaceuticals for her
assistance with the Antisense oligonucleotides and also thank Jong-Kook Park with
experiments assistance.
92
Figure 4.1. Flow cytometric analysis of the effect of antisense oligonucleotide (ASO)
on cell cycle of HS766T cells.
Histograms shown here represent 10,000 events and the percentage of each cycle is
calculated. (A) cell cycle of control oligo, 20 nM for 48 h. (B) cell cycle of ASO-miR-21,
IC50 for 48 h. (C) cell cycle of ASO-miR-301, IC50 for 48 h. (D) cell cycle of ASO-miR-
212, IC70 for 72 h.
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0
10
20
30
40
50
60
70
Anne
xin
V p
ositiv
e c
ells
(%
)
*
*
**
**
**
**
Contro
l olig
o (5
0nM
)
AS
O21 (4
5nM
)
AS
O212 (2
0 n
M)
AS
O221 (2
0 n
M)
AS
O301 (4
0 n
M)
Contro
l olig
o (5
0 n
M)
AS
O2
1 (4
5 n
M)
AS
O212 (2
0 n
M)
AS
O221 (2
0 n
M)
AS
O301 (4
0 n
M)
24 hr 48 hr
Figure 4.2. Flow cytometric analysis of apoptosis in HS766T cells.
Apoptosis induction in HS766T cells transfected with control oligo and antisense to miR-
21, -212, -221, or -301. Cells were treated with an IC70 concentration of each ASO.
Cells were harvested after 24 and 48 h of incubation and subsequently assayed for
apoptosis induction by flow cytometry using Annexin V-FITC staining.
* P < 0.05; ** P < 0.01 (Student’s t-test).
94
Figure 4.3. Western blot analysis of PTEN, RECK and p27(kip1) in HS766T cells
treated with antisense to miR-21 or miR-221.
Increased protein expression of the predicted targets to (A) miR-21(PTEN), miR-21
(RECK) and (B) miR-221(p27(kip1)) are shown. Numerical values represent the relative
levels of each protein to control protein (actin) as determined by densitometry.
95
(A)
(B)
Figure 4.4. Alignment of the FOXO3a binding site with miR-212 and mRNA
expression of FOXO3a after ASO treatment.
(A) Seed region complementarity between miR-212 and FOXO3a 3’UTR and
conservation of FOXO3a binding site across species. (B) The relative gene expression of
FOXO3a was measured after transfection. 20 nM of control oligo and ASO-miR-212
were used.
un
trea
tmen
t_24h
lip
o c
on
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l_24h
lip
o c
on
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l_48h
co
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Rela
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OX
O3
a m
RN
A leve
l
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1e-7
2e-7
3e-7
4e-7
5e-7
6e-7
7e-7
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Figure 4.5. Target validations by luciferase assay and FOXO3a western blotting.
(A) Renilla luciferase activity was normalized by firefly luciferase and cells were treated for 24 h. FOXO3a protein levels were
evaluated after ASO-miR-212 transfection at various time points (B) and transfecting pre-miR-negative (N) and pre-miR-212 (P) at 30
nM (C) and 60 nM (D). Numerical values represent the relative levels of each protein to control protein (actin) as determined by
densitometry.
97
CHAPTER 5
SYSTEMATIC EVALUATION OF microRNA PROCESSING PATTERNS
IN TISSUES, CELL LINES AND TUMORS
5.1 INTRODUCTION
The genes encoding microRNAs (miRNAs) are located within introns, intergenic
regions, and exons (15). The primary RNA transcript containing the miRNA primary
precursor (pri-miRNA) is processed in the nucleus by Drosha/DGCR8 to produce the
precursor miRNA (pre-miRNA) (19). pre-miRNA is ~70 nt in length and contains the
characteristic hairpin structure. The pre-miRNA is transported to the cytoplasm by
Exportin 5 (207), where it is subsequently processed by Dicer to the ~21 nt mature
miRNA.
Very little is known regarding the regulation of miRNA processing. Conceivably,
regulation of miRNA biogenesis may occur at the level of Drosha processing, nuclear
transport, or Dicer processing. Several studies have shown that miRNAs are present at
the level of precursor but are not processed to the mature miRNA. Michael et al. (76)
used Northern blots to show that the precursors to miR-143 and miR-145 are expressed in
colorectal tissues and tumors; however, the mature miRNA was detectable only in the
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normal colorectal tissue. Pre-miR-138-2 was shown by Northern blotting and in situ
hybridization to be expressed in HeLa cells, but the mature miR-138 was undetectable
(43). By using published mRNA data to approximate the miRNA precursor expression
encoded within introns, Thomson et al. (208) showed widespread down-regulation of
miRNAs in cancer. To our knowledge, no one has addressed the issue of relative
differences in precursor and mature miRNA expression by large-scale profiling. This
may be due to the fact that the majority of miRNA studies have focused on quantifying
the active mature miRNA and not the miRNA precursors.
In 2004, the Schmittgen laboratory developed the first real-time RT PCR method
to quantify miRNA (59). This method quantified the miRNA precursors with the goal of
predicting the expression of mature miRNA. By designing primers to the stem of the
miRNA hairpin, the primary precursor and precursor miRNA were simultaneously
quantified (59). This technology has been applied to profile the expression of over 200
miRNA precursors in a number of human cancer cell lines (59, 61, 209) and tissues from
patients with pancreatic cancer (64). In 2005, a TaqMan-based RT-PCR assay was
designed to quantify the mature miRNA (60). PCR and Northern blotting demonstrated
that the expression of mature and precursor miRNAs generally correlate (59, 61, 64, 110,
209). However, precursors to miR-1, miR-122a, and miR-133 showed considerable
expression by PCR, yet mature miRNA was undetectable by Northern blotting (61). This
prompted us to ask the following question: How widespread are alterations in miRNA
biogenesis in different tissues, tumors, or cancer cells lines? To address this question, we
profiled the expression of over 200 precursor and mature miRNAs using sensitive
realtime PCR assays. Our results demonstrate that a wide discrepancy exists between the
99
levels of precursor and mature miRNA, suggesting that unknown mechanisms that
control processing play a critical role in regulating the expression of active, mature
miRNA.
5.2 MATERIAL AND METHODS
5.2.1 Cells, tissues and RNA
Human cancer cell lines were obtained from ATCC or other investigators.
Human total RNA from 20 tissues was from Ambion. CD19+ human B cells were
purchased from AllCells. RNA from pancreas and liver tumor and adjacent benign
pancreas were obtained from our prior studies (64, 210). RNA was isolated from all
samples using Trizol per the manufacturer’s instructions. RNA purchased from Ambion
was isolated using ToTALLY RNA (Ambion). Both Trizol and ToTALLY RNA are
similar to the widely used guanidine thiocyanate, acid phenol:chloroform procedure of
Chomczynski and Sacchi (211) and will contain mature miRNA. RNA integrity was
evaluated by analyzing 50–100 ng of total RNA using the Agilent 2100 Bioanalyzer.
5.2.2 Real-time RT PCR, miRNA precursors, and mature miRNA
Expression data on 205 miRNA precursors in human cancer cell lines, pancreas,
and liver tissues were as previously reported (61, 64, 210). The expression of 201
miRNA precursors in the 21 normal human tissues was determined using real-time RT
PCR as previously described (59, 61). Mature miRNA expression in the normal tissues,
cell lines, pancreas, and liver tissues was determined using a real-time PCR assay to
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mature miRNA as described (60) with several modifications. cDNA to the mature
miRNA was synthesized using gene specific looped primers. Primers were grouped into
five portions of 48-plex each. Mature miRNAs were selected only to match the miRNAs
on which we had precursor data. Five different cDNAs were synthesized for each sample
using one of the five RT 48 plex primer mix as described (60).
5.2.3 Real-time RT PCR, absolute copy number for precursor and mature miRNA
Absolute copy number of miR-155 precursor was determined by constructing a
standard curve of pre-miR-155. Pre-miRNA RNA was synthesized using in vitro
transcription using primers that annealed to ~50 base pairs (bp) upstream of and down-
stream from the miR-155 hairpin. The T7 promoter sequence was added to 59 end of the
forward primer. Amplification of the pri-miRNA gene was performed by amplifying
genomic DNA (Roche) using standard procedures. The PCR product served as a
template for in vitro transcription. The transcription product was gel purified, and the
concentration was measured using a Nanodrop spectrophotometer. The reverse
transcription reaction was performed as described above, and standard curves of the log
of pre-miRNA-155 copy number versus the CT were made. We also generated a standard
curve for mature miR-155 as described (60). A minimum of 10 copies/cell was used as
the selection criteria for cutoff. The raw data set for the precursor and mature miRNA
expression levels is presented in the Appendix Table C.1-C.4.
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5.2.4 Northern blotting
Northern blotting was performed as previously described (2). LNA probes
specific to the mature miRNA were purchased from Exiqon.
5.2.5 In situ detection of miRNA
RT in situ PCR to detect the miRNA precursors was used as previously described
(64). The identical primers used to detect the miRNA precursors by real-time PCR were
used for the in situ RT-PCR. The only exception was miR-31; solution PCR used miR-
31 primary precursor primers, while the in situ PCR used precursor miR-31 primers. The
chromogen is nitroblue tetrazolium and bromochloroindolyl phosphate with nuclear fast
red as the counterstain. Therefore positive signal will appear blue, and a negative signal
will appear pink. Mature miRNA was detected by in situ hybridization using LNA
probes specific to the mature miRNA (Exiqon).
5.2.6 Data analysis
Pearson product moment correlation coefficients were determined for each pair of
precursor and mature miRNA using Microsoft Excel. The significance (probability) of
the correlation coefficients for each pair was determined from the t-statistic. The
probability of the t-statistic indicates whether the observed correlation coefficient
occurred by chance if the true correlation is zero. Correlation coefficient was considered
to be significantly different from zero at the 0.05 level (P ≤ 0.05). P values for all of the
correlations are located in the Appendix Table C.5 and C.6.
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5.3 RESULTS
The expression of 201 mature and precursor miRNAs was profiled in a panel of
22 human tissues, 37 human cancer cell lines, and pancreas and liver tumors/tissues using
real-time PCR assays specific to each class of miRNA (Appendix Table C.1-C.4).
Expression of the active, mature miRNA was either broadly expressed in each of the 22
tissues or was expressed among one, two, or three different tissues. Our mature miRNA
data in tissues closely resembled that of Liang et al. (212), who used the identical
TaqMan assay to profile miRNA in 40 normal human tissues. miRNA expression in the
tissues is designated as ubiquitous, tissue specific, or tissue enriched (Table 5.1).
Ubiquitous expression is designated as those mature miRNAs that are broadly expressed
in all or most tissues. Tissue-specific expression was defined as those miRNAs whose
expression is 20-fold or higher in the specific tissues compared with the mean of the
other tissues. For many specific miRNAs, the expression in the nonspecific tissues was
undetectable, so it was not possible to calculate a foldchange. The tissue-enriched
designation was reserved for mature miRNAs whose expression was less than 20-fold in
the enriched tissues compared with the mean of the other tissues. Tissues expressing the
most specific miRNAs include brain, heart, skeletal muscle, and pancreas (Table 5.1).
Several miRNAs have the ability to process different mature miRNAs from either
end of the same precursor. The mature miRNA is referred to as 5’ or 3’ (e.g., miR-142-
5p and miR-142-3p) or by the star designation (e.g., miR-9 and mir-9�) (12). TaqMan
assays were available to quantify 10 of these miRNAs, thus enabling us to study their
processing patterns in a variety of cell lines and tissues. Interestingly, the expression of
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both the 5’ and 3’ mature miRNA was detectable in most of the cell lines and tissues with
no apparent preference to process one or the other miRNA (Table 5.2). We also
compared the relative expression levels of both 5’ and 3’ miRNA. For some miRNAs,
the 5’ was expressed at somewhat higher amounts, and for other miRNAs, expression of
the 3’ was more predominate (Table 5.2). In some situations, only one of the two mature
miRNAs was detectable, for example, miR-302a and miR-302c but not miR-302a� or
miR-302c� (Table 5.2); however, this was rare. Asymmetric RISC assembly accounts
for one strand of the miRNA to accumulate (i.e., guide strand) and the other strand (i.e.,
passenger strand) to be degraded (31). Asymmetric RISC assembly likely accounts for
one of the two mature miRNAs to accumulate at slightly higher levels. However our data
demonstrate that both the 5’ and 3’ miRNAs accumulate in tissues at significant levels,
adding to the complexity of the activity of miRNAs.
The correlation between the expression levels of the precursor and mature
miRNAs was determined from the Pearson coefficient. The Pearson coefficient varied
widely among different cells/tissues and miRNA. For many miRNAs, the Pearson’s
correlation was > 0.6 (P < 0.05), demonstrating good correlation between the expression
of precursor and mature miRNA (Appendix Table C.5 and C.6). For other comparisons,
the Pearson value was near zero or negative, demonstrating that the expression of the
miRNA precursor did not correlate to the mature miRNA. miR-100 is an example of a
miRNA with good correlation between precursor and mature miRNA levels in the cell
lines, tissues, and tumors (Figure 5.1 A–C). One hundred twenty-five miRNAs displayed
good correlation (Pearson’s ≥ 0.3, P < 0.05) between the expression of the precursor and
mature miRNA (Appendix Table C.5 and C.6).
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Other situations displayed good correlation between the expression of precursor
and mature miRNA in specific tissues, while the expression of both precursor and mature
miRNA in the other tissues was very low or undetectable. These tend to be the tissue
specific miRNAs listed in Table 5.1 and were more prevalent among the tissue panel than
in the cell lines. Examples include miR-137 and miR-9 in brain and miR-1-1 in skeletal
muscle and heart (Figure 5.1 D–F).
Still other situations exist where high amounts of precursor were present in many
samples, yet mature miRNA was undetectable or had very low expression. The precursor
to the brain-specific miR-128a was expressed predominately in brain tissue, yet it was
expressed at ~100 copies per cell in many tissues (Figure 5.2A). Expression of the
mature miRNA was detectable only in brain and skeletal muscle (Figure 5.2B). miR-
128a precursor was present in all of the cancer cell lines at levels of several hundred
copies per cell, yet mature miR-128a was not expressed in any of these cell lines (Figure
5.3B). miR-105 had detectable precursor expression, at several hundred copies per cell,
in HS766T, Panc-1, MiaPaca-2, HCT-116, HeLa, and HL-60 cells, but these cells had
undetectable mature miRNA (Figure 5.3A).
The precursor PCR assay simultaneously measures the pri- and pre-miRNA (59).
We asked the following question: Is the mature miRNA absent in some of these cell lines
because the pri-miRNA is not processed by Drosha to the pre-miRNA? To answer this
question, we used Northern blotting to detect the pre-miRNA. Our experiments required
the enhanced sensitivity of LNA probes to visualize many of the miRNA precursors. The
precursor to miR-128a was present in four cancer cell lines and mouse brain; however,
the mature miR-128a was present only in mouse brain (Figure 5.3D). Precursor to miR-
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105 was clearly visible by Northern blotting in 6 cancer cell lines, but mature miR-105
was not (Figure 5.3C).
miR-31 was of particular interest because it displayed the greatest amount of
differential processing of any of the miRNAs studied. This differential processing was
validated by both real-time PCR and Northern blotting (Figure 5.4). Mature miR-31 was
detectable by real-time PCR and was clearly visible by Northern blotting in the cancer
cell lines HS766T and PL45 (pancreas) and HT-29 (colorectal) but not in the
hematological cell lines (K562 or HL-60) (Figure 5.4C,D). Mature miR-31 was
detectable at low to high levels in A549, HOP62, H719, MDA231, HeLa, PC3, and
HCT8 cells but was undetectable in MCF-7, LNCaP, and Daudi cells (Figure 5.4).
Interestingly the miR-31 precursor was detectable by Northern blotting in each of these
cell lines (Figure 5.4C,D). Our data clearly demonstrate situations where the miRNA
primary precursors are transcribed and processed to the precursor miRNA yet are not
processed to mature miRNA.
Precursor miRNA is generated by Drosha in the nucleus and then transported to
the cytoplasm by Exportin 5. Since Dicer processing occurs in the cytoplasm, we asked
the following question: Are certain miRNA precursors not processed to the mature
miRNA because they remain in the nucleus rather than being transported to the
cytoplasm? To answer this question, we applied in situ RT-PCR and in situ hybridization
to detect the precursor and mature miRNAs, respectively. For miRNAs that are
processed to mature (e.g., miR-31 in HS766T cells), the precursor staining was apparent
in both the nucleus and cytoplasm, while the mature miRNA was present only in the
cytoplasm (Figure 5.4E,F). For miRNAs that are not processed to the mature form, the
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precursor staining was primarily nuclear and/or nucleolar (Figure 5.3E,F and Figure
5.4G). This phenomena is particularly striking for miR-31 precursor expression in MCF-
7 cells; extensive positive staining for pre-mir-31 was apparent in the nucleolus only
(Figure 5.4G). MCF-7 cells are negative for mature mir-31 (Figure 5.4H). For miR-31,
miR-105, and miR-128a in these cell lines, processing to the mature form does not
proceed because the miRNA precursor is retained in the nucleus. The in situ PCR signal
is for the precursor miRNA (as well as the primary precursor) because the precursor
miRNAs were detected by Northern blotting (Figure 5.3 and 5.4).
The Pearson correlation was compared among the cell lines, tissues, and tumors
by plotting the frequency versus the Pearson coefficient (Figure 5.5). The cell lines
produced a unimodal distribution centering near zero (Figure 5.5A). The histogram for
the 22 normal tissues produced a bimodal distribution with one mode centered near zero
and the other greater than zero (Figure 5.5B). The pancreas and liver tissues also
produced conflicting histograms, with the liver specimens displaying a unimodal
distribution centering at zero while the pancreas tissues were bimodal (Figure 5.5C,D). A
high positive Pearson correlation demonstrates that the expression of precursor and
mature miRNAs are directly proportional and suggests efficient processing of precursor
to mature miRNA. A low Pearson coefficient suggests no relationship between the two,
one explanation being that precursors are not being processed to mature miRNA. A
negative Pearson suggests an inverse relationship between mature and precursor miRNA.
It should be emphasized that of over 800 comparisons, only 27 negative Pearson
coefficients were significant (P < 0.05). Our data suggest that processing of many
precursor miRNAs is somehow deficient or defective in cancer cell lines and liver tissues.
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However in 22 normal tissues and pancreas tissues, there is a population of miRNAs that
are processed efficiently. This trend parallels the miRNA expression pattern in tumors.
In pancreas cancer, there is a predominate increase in the expression of the miRNA (64),
while in liver cancer, the opposite is true (81, 210). This suggests that in pancreatic
cancer, the increased expression of miRNA is regulated at the level of transcription with
no apparent alterations in processing, but in liver cancer, the reduced expression of
miRNA in the tumors results from a reduction in miRNA processing.
It was recently demonstrated that mutations (153) or SNPs (213) within the
miRNA precursors can cause interference with miRNA processing. While we believed
this to be an unlikely explanation to describe the lack of miRNA processing, since the
phenomena was so widespread (Figure 5.3 and 5.4), we sequenced +100 bp on either side
of the miR-31 hairpin in HeLa and MCF-7 cell lines to look for evidence of mutations or
SNPs. The sequences were unchanged compared with wild type, demonstrating that
reduced levels of mature Mir-31 in these cells was not due to reduced processing caused
by a mutation or SNP.
5.4 DISCUSSION
The Pearson’s correlation data along with the relative expression levels of
precursor and mature miRNA demonstrated that biogenesis of miRNAs fit into different
categories. In one group of miRNAs, the precursor and mature miRNAs are expressed in
most tissues and cell lines, and the precursor expression correlates with the mature
miRNA expression. These miRNAs typically had a Pearson correlation coefficient > 0.3
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and P < 0.05. Examples of these miRNAs include many of the ubiquitously expressed
miRNAs such as miR-100, miR-155, and miR-125a. Expression of these miRNAs would
presumably be regulated by transcription alone with no regulation of processing events
(i.e., all miRNA expressed as primary transcripts are processed to mature miRNA).
Another group of miRNAs, such as many of the tissuespecific miRNAs, have a
high amount of expression of both the precursor and mature in specific tissues only. So
expression of these miRNAs is regulated by transcription in specific tissues with no
apparent regulation of miRNA biogenesis. Examples of these miRNAs include miR-9,
miR-1, and miR-216. Still another group of miRNAs expressed the precursor at
significant levels in all or most of the cells or tissues; however, the mature miRNA is not
present. Examples include miR-105, miR-128a, and miR-31 in cancer cell lines (Figure
5.3 and 5.4) and represent miRNAs that are expressed at the primary transcript level yet
for some unknown reason are unable to be processed to the mature miRNA.
Our data clearly show that a large number of miRNAs are transcribed as
precursors but are not processed to mature miRNA. Conceivably any of the steps
involved in miRNA biogenesis (nuclear transport or processing by Drosha or Dicer)
could be involved to reduce the mature miRNA levels. Reduced levels of Dicer were
shown in lung tumors (214), and many of the cancer cell lines studied here had reduced
mature miRNA. It is unlikely that a whole-scale reduction in Drosha or Dicer processing
accounts for the reduced levels of mature miRNA in cells that express the miRNA
precursors since other miRNAs are efficiently processed to mature in these same cell
lines or tissues. One explanation to account for reduced nuclear transport or processing
by Drosha or Dicer in some miRNAs but not others is that other specific factors bind to
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the miRNA precursors and either promote or interfere with processing or transport. In
fact, this inhibitory model was proposed by Obernosterer et al. (43) to account for a lack
of mature miR-138 in HeLa cells. It was recently shown that hnRNP A1 is required for
Drosha processing of miR-18 from the primary precursor (40). Conceivably, other
hnRNPs may be involved in regulating steps of miRNA biogenesis. While we did not
use Northern blotting and in situ hybridization to study all of the miRNAs with reduced
processing, the ones that were studied in several cancer cell lines showed that the miRNA
was processed to the precursor but was not transported from the nucleus to the cytoplasm.
This suggests an intriguing, unknown mechanism to guide the subcellular distribution of
miRNA precursors and prevent them from being processed to the active mature miRNA.
It is our hope that with the availability of this data set, many future studies will be
initiated to unravel the various mechanisms that control miRNA biogenesis.
5.5 ACKNOWLEDGMENTS
We thank Caifu Chen at Applied Biosystems for his assistance with the TaqMan
microRNA assays and also thank Gerard Nuovo for performing In situ RT PCR. This
work was supported by grant R21 CA114304 to T.D.S.
110
Figure 5.1. microRNAs with good correlation in expression levels of precursor and
mature.
The expression of the precursor and mature miRNAs was determined using real-time
PCR assays specific to each class of RNA. Plots of the correlation between the precursor
and mature miR-100 in a panel of 22 normal tissues (A), 37 cancer cell lines (B), and the
pancreas and liver tumors and normal tissues (C) are shown along with the Pearson
correlation coefficient. Also shown is the correlation in the expression of precursor and
mature miRNAs in the panel of 22 normal tissues for miR-1 (D), miR-137 (E), and miR-
9 (F).
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Figure 5.2. Expression of miR-128a in 22 normal tissues and cells.
The expression of miR-128a precursor (A) and mature miR-128a (B) was determined by
quantitative real-time PCR in 22 normal human tissues and cells.
112
Figure 5.3. Lack of microRNA processing in cancer cell lines.
The expression of precursor and mature miR-105 (A) and miR-128a (B) was assayed in
several human cancer cell lines by real-time PCR. mir-128a was also detected in mouse
brain tissue. Detection of the primary precursor (black columns) was apparent in all cell
lines; expression of mature miRNA (red column) was detectable only in the brain tissue.
With the exception of mouse brain tissue, Northern blots to miR-105(C) and miR-128a
(D) reveal the presence of the precursor only. Cell lines are the same as shown for the
quantitative PCR. In situ RT-PCR results for pre-miR-105 in HeLa cells (E, note intense
nuclear localization) and pre-miR-128a in HS766T cells (F).
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Figure 5.4. miR-31 expression in cancer cell lines.
The expression of precursor and mature miR-31 was assayed in 15 cancer cell lines.
Detection of the primary precursor (black columns) was apparent in all cell lines by real-
time RT-PCR (A, B); however, the mature miR-31(red columns) was present at varying
levels or not at all. (C, D) Northern blot confirmation of precursor and mature miR-31 in
cell lines. Note the presence of the ~70-nt precursor in each of the cell lines. Each
column of the Northern blot corresponds to the identical cell lines listed in panels A and
B. In situ RT-PCR to miR-31 precursor in HS766T cells (E) and MCF-7 cells (G). In
situ hybridization using an LNA probe to mature miR-31 in HS766T cells (F) and MCF-7
cells (H). Magnification, X400.
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Figure 5.5. Pearson correlation among the various samples studied.
The frequency of the Pearson’s correlation coefficient describing the relationship
between the precursor and mature miRNA expression is plotted for the cell lines (A), 22
normal tissues (B), normal pancreas and pancreas tumors (C), and normal liver and liver
tumors (D).
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Table 5.1. microRNA that display tissue-specific or tissue enriched expression.
Tissue specific expression.a
miR-122a (liver); miR-124a (brain); miR-128a (brain, skeletal muscle); miR-216, miR-217
(pancreas); miR-219 (brain); miR-302a, miR-302c (heart); miR-367 (heart); miR-373 (placenta)
Tissue specific expression.b
miR-1 (heart, skeletal muscle); miR-7 (brain, pancreas, thyroid); miR-95 (skeletal muscle);
miR-133a (heart, skeletal muscle); miR-149, miR-153 (brain); miR-203 (esophagus); miR-204
(kidney); miR-206 (skeletal muscle); miR-215 (small intestine); miR-302d (heart); miR-323,
miR-330, miR-346 (brain); miR-371, miR-372 (placenta); miR-375 (pancreas)
Tissue enriched expression.c let-7i (thyroid); miR-148a (pancreas); miR-150 (spleen, thymus, B cell); miR-181a (thymus);
miR-181b, miR-181c (brain, thymus); miR-190 (kidney); miR-192, miR-194 (colon, small-
intestine); miR-196b (cervix); miR-205 (esophagus, prostate, thymus); miR-213 (brain,
thymus); miR-223 (lung, spleen); miR-328, miR-338, miR-340 (brain); miR-376a (placenta)
Not expressed in any of the 22 tissues studied. miR-220, miR-302a�, miR-302c�, miR-384
Expression of 202 mature miRNAs was profiled in 22 human tissues and cells using real-time PCR as described in Materials
and Methods. aMature miRNA expression is detectable at sufficient levels in the specific tissue but is undetectable in all or most of the
remaining tissues. bMature miRNA expression is >20-fold higher in the specific tissue(s) compared with the mean of the other tissues.
cMature miRNA expression is <20-fold higher in the enriched tissue(s) compared with the mean of the other tissues.
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Table 5.2. Relative expression levels of mature miRNA and miRNA star forms.
miRNA (5’ and 3’)a
Expression ratio (5’ miRNA/3’ miRNA)b
miR-9, miR-9� 4.40
miR-17–5p, miR-17-3p 25.7
miR-126�, miR-126 -10.8
miR-142–5p, miR-142-3p -13.7
miR-154, miR-154� Not expressed
miR-199a, miR-199a� -162.4
miR-302a�, miR-302a miR-302a only expressed in heart
miR-302c�, miR-302c miR-302c only expressed in heart
miR-324–5p, miR-324-3p -3.0
miR-380–5p, miR-380-3p miR-380–3p not expressed
aMature miRNAs are listed in the order as they appear within the miRNA precursor
in the 5’ (5p) to 3’ (3p) orientation. bThe mean expression of the mature miRNA and miRNA� was determined in a
panel of 22 human tissues. The expression ratio is presented as the mature miRNA
that is located on the 5’ end of the miRNA precursor compared to that which is 3’
on the end.
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CHAPTER 6
SUMMARY AND DISCUSSION
The goal of this dissertation was to identify the most important microRNA
(miRNA) that can be used for diagnosis and treatment of pancreatic cancer. We
hypothesized that miRNAs are deregulated in pancreatic cancer and the inhibition of
upregulated miRNAs by antisense oligonucleotide would be a prospective therapy in
pancreatic cancer.
The summary of this dissertation is as follows; Expression profiling of a small
number of miRNAs (~ 200 miRNAs) distinguished pancreatic tumors from
normal/benign pancreas. Large numbers of miRNAs were differentially expressed in
pancreatic adenocarcinoma and the majority was increased in pancreatic cancer. In situ
RT PCR demonstrated that overexpressed miRNAs are restricted to pancreas tumors, not
stroma cells (Chapter 2). Overexpressed miRNAs were inhibited by antisense
oligonucleotides at low nM concentration and normal pancreatic cell lines (HPNE)
showed less sensitivity to antisense oligonucleotides, suggesting selective target therapy
in pancreatic cancer. The combination of antisense oligonucleotide with a
chemotherapeutic agent, gemcitabine showed sensitization effect via schedule-dependent
manner and the combination with two antisense oliogonucleotides also showed a
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synergistic effect (Chapter 3). In addition, miRNA inhibition by antisense
oligonucleotide caused cell cycle arrest and increased apoptotic cells. For further
mechanism study, the target molecules of miRNAs that are deregulated in pancreatic
cancer were validated. PTEN and RECK were confirmed as a target of miR-21 and
p27(kip1) was validated as a target of miR-221. FOXO3a was identified as a new target
gene of miR-212 using reporter assay and western blotting (Chapter 4). Lastly, the
processing of miRNA was studied in various normal tissues, patients’ tumor tissues and
cancer cell lines. A number of miRNAs showed tissue-specific expression and the
miRNA processing pattern was different in various samples (Chapter 5).
There are a number of evidences that miRNAs are differentially expressed in
many human cancers (reviewed in Chapter 1). Our study suggested that overexpressed
miRNAs in pancreatic cancer could be used as a therapeutic agent and diagnostic in
pancreatic cancer. Although many miRNAs that were differentially expressed in
pancreatic cancer were also implicated in other type of cancers (i.e. miR-15, 16, 21, 155
and 221), several other miRNAs such as miR-212 and 301 appear to be deregulated
specifically in pancreatic cancer. Also, the cell proliferation assay using antisense
oligonucleotide indicated that low nM range of IC50 is effective to show cell cytotoxicity,
specifically in pancreatic cancer cells, not normal pancreas cells. These data indicate that
selective therapy targeting the miRNAs is promising. To better understand the miRNA
function, miRNA targets need to be validated. Here, one target molecule of miR-212
(FOXO3a) was identified possibly affecting cell cycle arrest and apoptosis in cancer cells.
Since one miRNA targets numerous mRNAs, it is a big challenge to identify targets and
to understand the network between miRNA and mRNA. Even, miRNA processing was
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impaired in certain samples or conditions, implying more complicated function of
miRNAs. This study is the first to show miRNA inhibition by antisense oligonucleotide
in pancreatic cancer and to identify a target gene of miR-212 in pancreatic cancer.
The possibility of miRNAs as a therapeutic agent was some what confirmed in
our studies. The inhibition of miRNAs by ASO showed that reduced cell proliferation,
cell cycle arrest and increased apoptotic cells in vitro. Even though we haven’t
confirmed yet whether ASO inhibits those miRNAs efficiently in vivo, Krützfeldt et al.
demonstrated that cholesterol conjugated antisense inhibited liver specific miR-122 in
mouse for 23 days (127). Currently, modified antisense has been developed for stable
transfection in vivo and if antisense is conjugated with molecules that are present only in
the pancreas it might be a new form of selective targeting to pancreatic tissue.
Even though miRNAs are important targets for therapeutic purposes, miRNAs
can also be used for diagnostic purposes. The survival rate in pancreatic cancer is very
low because most pancreatic cancers are diagnosed at very late, untreatable stage. From
our study, miRNAs that are overexpressed in pancreatic cancer could be a promising
diagnosis marker in pancreatic cancer.
One of the problems of diagnosing in pancreatic cancer is distinguishing chronic
pancreatitis from pancreatic cancer. It is reported that 5% ~ 10% of resected pancreatic
tissues are eventually diagnosed as pancreatitis, not as pancreatic cancer (216).
Pancreatic cancer and chronic pancreatitis show similar symptoms and clinical data (217).
Chronic pancreatitis contains abundant stroma, similar to pancreatic cancer (146), but the
treatment of chronic pancreatitis is very different from pancreatic cancer. In our study,
we profiled ~ 200 precursor miRNAs on 4 chronic pancreatitis samples to see if miRNA
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expression is different from cancer. Clustering data (Figure 2.2) showed chronic
pancreatitis is separated from the cancer group and terrain map (Figure 2.3) showed
chronic pancreatitis lie in between the normal pancreas and tumor. This result suggests
that miRNA may be a good marker to distinguish the pancreatic cancer and chronic
pancreatitis. Mature miRNA profiling data with sufficient samples are needed and
microdissected tumor and stroma samples may provide a better miRNA marker for
chronic pancreatitis and pancreatic cancer.
In addition, pancreatic juice has been used to investigate the genetic markers of
pancreatic cancer (218). Pancreatic juice can be collected in the duodenum during
routine upper gastrointestinal endoscopy after secretin stimulation (219). No one has
systemically profiled miRNAs in pancreatic juice samples and this method will be an
easier diagnostic for patients compared to tissue biopsy to screen the cancer marker.
The pancreatic tissue and juice samples are directly obtained from pancreas, but
tissue biopsy or GI endoscopy is not a convenient or economic procedure for the patient.
Serum is the ideal clinical specimen because it is easily obtained from patients and once a
diagnostic assay is developed, patients can have more frequent examination for early
diagnosis. However, serum samples are not from direct tissue, so pancreatic tissue or
juice might show more accurate diagnostic marker, which means a more sensitive serum
marker is needed. As mentioned in Chapter 3, serum marker CA19-9 (sialylated Lewis
blood group antigen) has been used, but the usage is limited because it shows a high
false-positive rate and some patients do not express Lewis antigens (154-156). Recently,
serum macrophage inhibitory cytokine 1 (MIC-1) seems to be a better marker of
pancreatic cancer than CA19-9 (157). However, MIC-1 is not better than CA19-9 in
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discriminating chronic pancreatitis patients from pancreatic cancer patients (157). Our
study showed that miR-216, 217 and 375 were specifically expressed in pancreas
(Chapter 5) and miR-212 and 301 were overexpressed in pancreatic cancer (Chapter 2).
If these miRNAs are differentially expressed in patients’ serum samples, this might be the
most sensitive and accurate diagnostic marker for pancreatic cancer.
Lastly, pancreatic cancer precursor lesions can be useful for early detection of
cancer. Pancreatic ductal adenocarcinomas arise from noninvasive epithelial precursor
lesions and one of the epithelial precursors in the pancreas is pancreatic intraepithelial
neoplasm (PanINs), which can be used for a pancreatic cancer progression model (215).
This model contains a series of precursor lesions from low-grade PanIN-1A to high-grade
PanIN-3. PanINs are common in invasive ductal adenocarcinoma and many of the
genetic changes in cancer-related genes are shown in these lesions (Figure 6.1). PanIN-
1A is flat epithelial proliferation that doesn’t contain architectural or cytologic atypia and
PanIN-1B is similar with PanIN-1A, but shows intraluminal papillae. The mutations of
K-ras and HER-2/neu genes usually occurr in the early stage of PanINs. The tumor-
suppressor gene p16 is inactivated in PanIN-2, which shows moderate nuclear and
architectural atypia. In PanIN-3 stage, p53, DPC4, and BRCA2 genes are decreased and
there are significant architectural and cytological atypia that nuclei are enlarged and
overlapping. In our study, the expression of tumor suppressor gene PTEN, RECK, p27
and FOXO3a were repressed by miR-21, 221 and 212. We haven’t examined which
stage these miRNAs occur. It is possible that these oncogenic miRNAs might function in
late stage of PanINs because tumor suppressor genes such as p16 and p53 are altered in
these stages (Figure 6.1). In addition, overexpressed miRNAs in our study might be
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targeting other tumor suppressor genes such as p53, DPC4, and BRCA2. To answer
these questions, a murine PanIN model, which are much alike in histology with human
PanIN, could be used to identify specific miRNAs in pancreatic cancer progression.
In conclusion, the study of miRNA expression and function in pancreatic cancer
suggests that miRNAs play an important role in tumor biogenesis and have a potential for
therapeutic and diagnostic agents.
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Figure 6.1. Pancreatic cancer progression model and genetic alteration.
Oncogenic miRNA-21, 221, and 212 repress tumor suppressor genes that cause cell cycle
arrest and apoptosis and they might be specific miRs in cancer progression and could be
better marker. ? : not confirmed yet.
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147
APPENDIX A
DATA RELEVANT TO CHAPTER 2
148
Tissue Type Chronic pancreatitis Normal Pancreas
miRNAs CP1 CP4 CP6 CP7 Ambion1 (N) Ambion2 (N)
let-7a-1* 0.012767564 0.004554593 0.010716357 0.005650276
let-7a-2-P 0.06314825 0.024298885 0.034370636 0.016007037 0.03634888 0.034601248
let-7a-3* 0.022277452 0.007258358 0.018295382 0.008624916 0.005953737 0.00733402
let-7b* 0.020109563 0.008586259 0.020264444 0.009398919 0.002387898 0.004160942
let-7c* 0.000543603 0.000190536 0.000434601 0.000198266
let-7d* 0.067560629 0.016552798 0.03898443 0.023946308 0.002639577 0.013907924
let-7e* 0.001953556 0.001065562
let-7f-1* 0.090772624 0.016667845 0.079698693 0.036982564 0.009458314 0.006559961
let-7f-2* 0.001815394 0.001042869 0.001410304
let-7g* 0.02490334 0.003639678 0.01497907 0.015056488 0.006369932 0.009764104
let-7i* 0.016838315 0.006728574 0.013672666 0.004408677 0.004876282 0.011195291
MIR-001-1 0.006010193 0.004590641 0.002594387 9.81398E-05 0.00099491 0.000930663
MIR-001-2 0.041960177 0.007066112 0.036118865 0.007905583 0.020764554 0.017999122
MIR-007-1 0.001198204 0.001263922 0.002724717 0.002435421 0.007157494 0.002328745
MIR-007-2 0.00022334 0.000236127 0.000747346 0.001181303 0.008283994 0.001740222
MIR-007-3 0.066299558 0.022849619 0.083151717 0.071358573 0.338322544 0.236435712
MIR-009-1-P 0.042820043 0.03842686 0.041434789 0.014831626 0.257808586 0.164846752
MIR-009-2-P 0.030410789 0.016794899 0.016108008 0.020724227 0.072412457 0.056627214
MIR-009-3-P 0.004868059 0.001665171 0.000395768
MIR-010a,b 0.000255121 0.009949689
MIR-015a 0.006271364 0.002268153 0.010861246 0.000420431 0.009179118 0.007294283
MIR-015b 0.007608744 0.00967714 0.001799911 0.000589615 0.006865729 0.015960159
MIR-016-1 0.014258866 0.014620986 0.030510124 0.017130381 0.019011395 0.010796828
MIR-016-2 0.097736996 0.103802955 0.089869873 0.041846625 0.101426442 0.069059053
MIR-017-P 0.067500597 0.008675703 0.041673089 0.004841798 0.055632402 0.054880421
MIR-018 0.710241994 0.046432247 0.545149137 0.168437418 0.253759848 0.241946399
MIR-019a-P 0.156661987 0.013489642 0.106883375 0.018285629 0.008367024 0.099447014
MIR-019b-1-P 0.005065515 0.004846943 0.00568522 0.000321186 0.02919225 0.023905516
MIR-019b-2-P 0.000382308 0.000369341
MIR-020 0.000315185 0.000163262 0.000147853 0.000369211
MIR-021 2.341142206 0.484202999 1.813199124 0.467826363 2.001817175 2.956513509
MIR-022-P 0.036732593 0.019780181 0.013561291 0.009714151 0.021133181 0.019293649
MIR-023a 0.053611733 0.01947956 0.049424267 0.02630918 0.013086115 0.009449887
MIR-023b-P 0.152687733 0.083139473 0.063950297 0.035967509 0.037915255 0.14623336
MIR-024-1,2 4.022794578 1.797990526 2.617194909 2.221679286 1.262563736 1.213713886
Continued
Table A. microRNA precursor expression in pancreatic tissues and cell lines. The expression of each miRNA precursor relative to 18S rRNA was determined using
real-time PCR as described in Materials and Methods. The data for each miRNA
precursor was determined using SYBR green detection on all miRNAs except those
with an asterik (*) which were determined using TaqMan probes. The expression of
each mature miRNA relative to 18S rRNA was determined using real-time PCR as
described in Materials and Methods. Data are presented as 2^-∆CT (CT miRNA - CT
18S rRNA) X 1,000,000. The data on 18S rRNA is the raw CT value.
149
Table A. continued
MIR-025 0.014534236 0.002017191 0.021287722 0.009170814 0.010690275 0.026596429
MIR-026a-1,2 0.010159932 0.004278047 0.003379688 0.002254476 0.001348446 0.003898703
MIR-026b 0.061031192 0.066914743 0.060933139 0.030577008 0.091468241 0.087280028
MIR-027a, b 0.043054581 0.00702677 0.024868156 0.016746479 0.020994121 0.028610011
MIR-028 0.002108041 0.0002481 0.002824025 0.000113562 0.001476794 0.000725965
MIR-029a,c 0.036616687 0.024450163 0.024079101 0.025740809 0.006679354
MIR-029b-1,2 0.000447457 0.000350692
MIR-030a,e 0.009098381 0.028338288 0.006146813 0.011618504 0.005538956 0.006441949
MIR-030b 0.057518827 0.036746545 0.069638762 0.044324666 0.042986329 0.06069794
MIR-030c-1,2 0.004284252 0.009825101 0.021738307 0.015281528 0.011527214 0.092756543
MIR-030d 0.043036247 0.005221449 0.004020804 0.006113272
MIR-031-P 0.038013945 0.043783555 0.040284213 0.011792264 0.189825896 0.19217051
MIR-032 0.01136044 0.013064081 0.004168735 0.001263644 0.006806407 0.001915632
MIR-033 0.000928967 0.000349703 0.000497972 0.00042864 0.000153537
MIR-034a 0.001606097 0.001025468 0.007751783 0.000470035 0.034041259 0.007937255
MIR-034b 0.00056078 0.000640861 0.000383238 0.000305746 0.004631787 0.002595809
MIR-034c
MIR-092-1 0.00467656 0.000575484
MIR-092-2-P 0.00028443 0.000986795 0.000732006 0.010868467 0.005224251
MIR-093 0.004549785 0.002066059 0.005246143 0.000678746 0.026074677 0.018327507
MIR-095 0.008550817 0.000237352 0.000619099 0.003892058
MIR-096-P 0.135395629 0.166538705 0.122449295 0.058341046 0.691508178 0.548908989
MIR-098 0.395513288 0.200547922 0.282877605 0.150995878 0.348510546 0.420654846
MIR-099a 0.00068848 0.000449164 0.000449417 0.000601951 0.004451034 0.002625458
MIR-099b 0.052199058 0.020044676 0.050994691 0.031251431 0.051381301 0.029222843
MIR-100 0.011726527 0.002945217 0.012368136 0.010485966 0.003268844 0.021370743
MIR-101-1 0.001256223 0.000639224 0.001044442 0.000164776 0.000235339 0.00048706
MIR-101-2 0.067390058 0.025316932 0.057451341 0.015532023 0.151054254 1.752910539
MIR-103-1,2 0.113320955 0.040466038 0.059308713 0.068214562 0.203195269 0.140712156
MIR-105-1,2 0.008403084 0.013297846 0.004531591 0.002389855 0.013743099 0.005700536
MIR-106a-P 0.000346189 0.000268811 0.001528436 0.000535674
MIR-106b 0.029821499 0.013409037 0.028810417 0.009571024 0.035342018 0.080899347
MIR-107 0.161549262 0.074540738 0.169600799 0.08313928 0.231565418 0.15655515
MIR-108 0.005332403 0.008613235 0.01351545 0.002144737 0.008016303 0.012246038
MIR-122a 0.000271888 0.000168237 0.000202524 0.001497577 0.000744918
MIR-124a-1,2,3 0.000271765 0.005854591 0.00560694
MIR-125a 0.037638686 0.007781262 0.028077492 0.001526758 0.068244151 0.055909214
MIR-125b-1 0.028684567 0.020528989 0.004835816 0.008964481 0.012792711
MIR-125b-2 0.000318138 0.00033646 0.002826068 0.000474395 0.001900974 0.003763557
MIR-126 0.031139435 0.018682553 0.010326938 0.012578255 0.107276146 0.024853897
MIR-127 1.443391405 1.322518514 1.338535006 0.652239432 1.228175066 1.193624348
MIR-128a 0.01308109 0.01497742 0.003764339 0.012932415 0.011828256 0.005029126
MIR-128b 0.00389817 0.003606393 0.002140326 0.001638763 0.008546143 0.008074703
MIR-129-2 0.04255903 0.053792447 0.039322391 0.012627467 0.285810578 0.214750124
MIR-130a-P 0.05095675 0.073840054 0.056078589 0.012432141 0.252355112 0.177391264
MIR-130b 0.000174327 0.000389355 0.001809944 0.001542816 0.001823327
Continued
150
Table A. continued
MIR-133a-1,2 0.025152857 0.006156363 0.010857505 0.004650737 0.032489475 0.011994326
MIR-133b 0.02396675 0.021021551 0.00931909 0.003391111 0.048682674 0.023065036
MIR-134 0.018352025 0.019122731 0.018976627 0.01182464 0.136395478 0.255991421
MIR-135a-1,2 0.000934791 0.000803997 0.020177299 0.005874401 0.001761802 0.007305083
MIR-135b-P 0.00118657 0.002349502 0.001346749 0.00614135 0.017659866 0.005187981
MIR-136 0.006804062 0.002207299 0.020303354 0.007678385 0.003676769 0.082237423
MIR-137 0.000447471 0.00103947 0.000883174 0.001245746 0.000575506 0.001122707
MIR-138-1,2 0.001714094 0.000262827 0.001397973 0.00044973 0.00093339
MIR-139 0.025153005 0.016604429 0.020384394 0.006667056 0.106312064 0.061125699
MIR-140
MIR-141 0.004501049 0.0086425 0.003534605 0.005206018 0.013817426 0.019355198
MIR-142-P 0.00339802 0.008042978 0.010785362 0.000911796 0.115605763 0.166751526
MIR-143 0.00369372 0.001751684 0.004817672 0.00097074 0.016971657 0.012679392
MIR-144 0.000273698 0.000420075 0.000233496 0.00743239
MIR-145-P 0.031911651 0.022850047 0.024736512 0.013065508 0.020027785 0.013671064
MIR-146 0.001451618 0.000251733 0.00673389
MIR-147 0.002270519 0.001758809 0.000515769 0.00221494 0.006845594
MIR-148a-P 0.002155628 0.002563387 0.003181465 0.001131528 0.008784268 0.007050901
MIR-148b-P 0.037287376 0.017141687 0.045625949 0.033291209 0.004765249 0.047246831
MIR-149 0.015495854 0.008078378 0.015534955 0.00936547 0.113169941 0.069601122
MIR-150 0.003753128 0.006012553 0.001885013 0.001145313 0.028573998 0.026609088
MIR-151 0.012107099 0.009395958 0.011398042 0.003513248 0.000953368 0.00487287
MIR-152 0.008298464 0.001777718 0.007610349 0.013484572 0.011065352 0.005798285
MIR-153-1,2 0.00346641 0.019314136 0.014266135 0.000216381 0.007504883 0.000905268
MIR-154 0.009455281 0.002693799 0.006473768 0.011609966 0.013345639 0.140458892
MIR-155 0.006474987 0.011309025 0.000104749 0.014553467 0.002732431
MIR-181a,c 0.016747454 0.015833126 0.014009326 0.002935386 0.014163854 0.006231712
MIR-181b-1,2 0.001071404 0.000394636 0.000714214 0.000402429 0.00082498 0.002324979
MIR-182 0.114077333 0.039264489 0.063994395 0.095568075 0.044764448 0.049877168
MIR-183 0.000176519 0.000759672 0.002275914 0.004266724 0.000981441 0.000911294
MIR-184 0.013735575 0.015170747 0.004686897 0.003159899 0.047283535 0.057244777
MIR-185 0.265489106 0.25329402 0.205033332 0.142422656 0.28688426 0.264546433
MIR-186-P 0.249354314 0.238891499 0.343926782 0.310028354 0.131957908 0.192107782
MIR-187 0.000169812 0.003297623 0.000112497 0.003482237 0.001683475
MIR-188b-P 0.000272428 0.000597614 0.000910898 0.001806537 0.002886692
MIR-190 0.00017583
MIR-191 0.000731131 0.000607368 0.000391679
MIR-192 0.000699001 0.005158609 0.001272982 0.000278215 0.004109972 0.022998034
MIR-193 0.002903987 0.000351593 0.006451089 0.005409161 0.005158557 0.011033806
MIR-194-1,2 0.000252311 0.000568257 0.000765849 0.002568362 0.00080106
MIR-195-P 0.033704664 0.064680131 0.055662456 0.021886735 0.323387702 0.366278232
MIR-196a-1 0.000388584 0.000304802 0.000741552 0.000111348 0.004269769 0.009067291
MIR-196a-2 0.003463811 0.001560232 0.001208741 0.001227677 0.002913668 0.003392027
MIR-196b 0.002501064 0.013480959 0.001895779 0.001053794 0.056271578 0.022356015
MIR-197 0.039397436 0.00767588 0.046983349 0.012516197 0.120997632 0.198911952
MIR-198 0.002505612 0.001030131 0.004541608 0.000452906 0.00298949 0.001381301
Continued
151
Table A. continued
MIR-199a-1-P 0.298467256 0.25086062 0.166578992 0.092487139 0.433476359 0.360208809
MIR-199a-2-P 0.027350084 0.000421998 0.016245933 0.001672517 0.006325522 0.015860102
MIR-199b-P 0.094182157 0.067465461 0.068591441 0.02481399 0.156936215 0.150919054
MIR-200a-P 1.040433725 1.097327693 0.927129884 0.357621176 3.561935073 2.349448867
MIR-200b 0.005236239 0.00882399 0.005748241 0.005008713 0.008166116 0.013887001
MIR-200c 0.001875655 0.002254475 0.002430292 0.001505718 0.007420339 0.006308269
MIR-203 0.000497538 0.000222475 0.000152136
MIR-204 0.008004919 0.012991352 0.012451945 0.004396321 0.065474308 0.04547774
MIR-205 0.004022595 0.003139284 0.000884591 0.000333252 0.000239987 0.000250098
MIR-206 0.002138564 0.000282114 0.002042835 0.006840329
MIR-208 0.000733871 0.007252755 0.000367513 0.001065468 0.00897855 0.002034795
MIR-210 0.09111678 0.044985782 0.043284832 0.012617147 2.13176546 0.754484605
MIR-211 0.073901403 0.006813515 0.050940321 0.026306618 0.277102997 0.124284679
MIR-212 0.170904889 0.009373009 0.115209842 0.028910654 0.019571386 0.024553472
MIR-213 0.002785865 0.003108049 0.00200287 0.00070607 0.013444866 0.001219855
MIR-214 0.067405311 0.06752029 0.067519399 0.033686739 0.107147472 0.121379179
MIR-215 0.005950176 0.005737333 0.012282213 0.004135509 0.000180488 0.005897319
MIR-216 0.290399841 0.066188341 0.135031778 0.053202619 0.023985374 0.796469692
MIR-217 0.000235323 0.000170291 0.000180144 0.00165481
MIR-218-1,2 0.008985653 0.008121506 0.01599596 0.001907882 0.011345368 0.00237825
MIR-219-1,2 0.000752839 0.000781918 0.006967928 0.000183472 0.001300564 0.000636546
MIR-220 0.000649481 0.000380729 0.000707701 0.00193472 0.003250969 0.002219262
MIR-221 0.009422457 0.001132225 0.012976988 0.001583838 0.001503949 0.003099277
MIR-222-P 0.010800759 0.000349949 0.005152114 0.003193503 0.0025027 0.0047615
MIR-223 0.000174591 0.000475107 0.002287581 0.000896812
MIR-224 0.025570773 0.014669204 0.020176872 0.011781545 0.024495308 0.015615969
MIR-296 0.003529366 0.012403017 0.002707379 0.000628015 0.004989904 0.007946184
MIR-299-P 0.015990563 0.017543379 0.015012946 0.002988038 0.039464444 0.041617262
MIR-301 0.010070304 0.007361512 0.000388674 0.021308422 0.002189063 0.000468284
MIR-302 0.01186985 0.006763867 0.000808252 0.005987218 0.002020106 0.001943118
MIR-302b 0.009426909 0.009476736 0.006532407 0.004181685 0.014986297 0.010019941
MIR-302c 0.000259907 0.000742716 0.00141125 0.004454245 0.001977327
MIR-302d 0.002969431 0.000343145 0.001321731 0.000104538 0.005817989 0.006271514
MIR-320 0.007278607 0.001174052 0.001375367 0.00141726 0.019171901 0.006686201
MIR-321 0.503973775 0.572789686 0.726818914 0.70760864 1.844779286 1.4878209
MIR-323 0.042517514 0.014855041 0.00657378 0.020966772 0.071572775 0.124469517
MIR-324-P 0.00365075 0.008846233 0.01163056 0.002844806 0.057851482 0.0335496
MIR-325 0.00443695 0.004339746 0.002946879 0.002596601 0.018084716 0.008847416
MIR-326-P 0.002547828 0.000698274 0.002562807 0.000540673 0.003020398 0.006940688
MIR-328 0.204784023 0.133565649 0.124115988 0.060584396 0.519801326 0.137784391
MIR-330 0.001550312 0.002652331 0.003890964 0.004113695 0.003163172 0.013502907
MIR-331-P 0.018226976 0.022177498 0.013292796 0.00801185 0.084372445 0.119610369
MIR-335 0.000312403 0.001414699
MIR-337 0.003370729 0.004834715 0.002915711 0.000719814 0.030593664 0.034853802
MIR-338 0.005481172 0.013365456 0.004720068 0.000785842 0.000259684 0.00033258
MIR-339 0.308455883 0.19562273 0.207006844 0.104573707 0.124672201 0.05196366
Continued
152
Table A. continued
MIR-340 0.049059593 0.0378978 0.05342104 0.031652792 0.056894942 0.047978933
MIR-342 0.006266854 0.00277656 0.005425455 0.000138995 0.010622662 0.009543253
MIR-345 0.038088996 0.060830468 0.019169923 0.009714649 0.270400968 0.349214863
MIR-346 0.04285465 0.015982046 0.018191917 0.023584676 0.092284631 0.087633717
MIR-361 0.00429402 0.011935766 0.002435201 0.000983947 0.0168768 0.013473494
MIR-367 0.005381289 0.000687387
MIR-368 0.002403004 0.00279905 0.003731997 0.001802181 0.006710613 0.004295533
MIR-369 0.035557566
MIR-370 0.064662207 0.017665837 0.067686052 0.025862769 0.073265952 0.565532315
MIR-371 0.010450665 0.015527791 0.003743435 0.001098226 0.06270993 0.08601612
MIR-372 0.001638097 0.000154308
MIR-373 0.002161575 0.003207945 0.003245283 0.000257784 0.003133078 0.00899273
MIR-374-P 0.00108488
MIR-375 0.09199046 0.020887258 0.076421181 0.036105478 0.044454914 0.078275215
MIR-376 0.144953399 0.055565489 0.053468328 0.080314571 0.020308574 0.318603246
MIR-377 0.028962703 0.002024902 0.001257267 0.001239998 0.008179125 0.041882631
MIR-378 0.000456763 0.001340092 0.00061336 0.000603437 0.005806966 0.005676372
MIR-379 0.048961856 0.036919925 0.049887852 0.040966972 0.07170906 0.284864524
MIR-380 0.011264273 0.007518173 0.009380535 0.00919796 0.00197598 0.023827351
MIR-381 0.000455134 0.001046901 0.000532353 0.000771986 0.002547867
MIR-382 0.000798927 0.000181261 0.000189964 0.000419186 0.02977139
MIR-383 0.001573386 0.00062277 0.000602749 0.000579965 0.006042031 0.006407707
MIR-384 0.000189276
MIR-422b 0.003561243 0.002401419 0.004134326 0.001615879 0.006772073 0.017108082
MIR-423 0.005969613 0.003617062 0.001078241 0.001447259 0.012702645 0.009890974
MIR-424 0.008935437 0.007596207 0.001936085 0.002342667 0.000832088 0.000381683
MIR-425 0.000207824 0.004474417 0.003891083 0.003835097
18S rRNA (CT) 7.4282695 7.16372935 7.1029398 6.620629 6.96878565 6.9713573
Tissue Type Normal Pancreas Adjacent Benign
miRNAs Ambion3 (N) Ambion4 (N) BD(N) ST(N) OUP2 (B) OUP16 (B)
let-7a-1*
let-7a-2-P 0.023460053 0.036785208 0.029860799 0.02020963 0.037667539 0.093053858
let-7a-3* 0.002120054 0.012402666 0.001374538 0.007318707 0.01802732
let-7b* 0.001147644 0.004249536 0.001646644 0.010710603 0.019361678
let-7c* 0.000233558
let-7d* 0.009596833 0.003219003 0.003923437 0.018450767 0.010403781
let-7e*
let-7f-1* 0.010351675 0.005823148 0.004188598 0.009445643 0.01896164 0.021296686
let-7f-2*
let-7g* 0.011776659 0.004639262 0.001956045 0.002432392 0.017344566 0.01545451
let-7i* 0.015881941 0.00541939 0.010567787 0.006528355
MIR-001-1 0.001803267 0.002863931 0.005548375 0.001255527 0.009524411 0.002519756
MIR-001-2 0.010873115 0.00493528 0.011064788 0.033083994 0.051208019 0.036468781
MIR-007-1 0.002328092 0.000603521 0.007105338 0.003188275 0.021646972 0.008558352
MIR-007-2 0.009572003 0.006148408 0.001458586 0.002237086 0.0057143 0.010822801
Continued
153
Table A. continued
MIR-007-3 0.156077402 0.174041959 0.010044876 0.05389782 0.42119379 0.445348077
MIR-009-1-P 0.273344655 0.11382549 0.152454757 0.266531919 0.285545701 0.612935117
MIR-009-2-P 0.062292124 0.06740517 0.045638623 0.068708134 0.124172884 0.122161854
MIR-009-3-P 0.003210086 0.000758332 0.000894607 0.001112762 0.00101393 0.006873555
MIR-010a,b 0.005657954 0.000151693 0.005058089 0.003654635 0.011068939
MIR-015a 0.009824138 0.007794737 0.001693084 0.0005465 0.017652105 0.023057338
MIR-015b 0.001427992 0.006178981 0.000173985 0.004656992 0.015822222 0.037806622
MIR-016-1 0.018770737 0.012843548 0.003367142 0.001493087 0.058034478 0.080209658
MIR-016-2 0.113455273 0.229469222 0.121675033 0.099270868 0.151546829 0.249867258
MIR-017-P 0.053755142 0.050202519 0.086076461 0.074940334 0.066453089 0.13410294
MIR-018 0.204774868 0.140910602 0.066609099 0.102862264 0.334647367 0.395013337
MIR-019a-P 0.072782609 0.001250622 0.019498792 0.000303401 0.042358508 0.134387166
MIR-019b-1-P 0.047490856 0.025862545 0.030810504 0.04124096 0.030029943 0.042803031
MIR-019b-2-P 0.00052715 0.000163743 0.001892753 0.000496062
MIR-020 0.000303129 0.002960546 0.000264216 0.00016767 0.000743265
MIR-021 1.501904488 1.200033823 0.035227199 0.533652763 5.56391573 3.534118144
MIR-022-P 0.025448626 0.008153166 0.044840326 0.036020318 0.025029514 0.06061866
MIR-023a 0.024108648 0.016621675 0.021428439 0.019036799 0.049623716 0.040917314
MIR-023b-P 0.079367522 0.062571525 0.064043172 0.022874051 0.13764824 0.214746313
MIR-024-1,2 1.425510899 0.656514404 0.607524531 0.912583971 5.816894221 4.507791443
MIR-025 0.105448397 0.00904403 0.011247803 0.012385116 0.012075561 0.025820545
MIR-026a-1,2 0.005033104 0.004790236 0.00698449 0.006123918 0.004343039 0.004452315
MIR-026b 0.115073383 0.112902949 0.129196695 0.131683749 0.188996192 0.276350719
MIR-027a, b 0.03889639 0.01301159 0.042972174 0.030975937 0.064792834 0.067463841
MIR-028 0.004468245 0.001008326 0.005605024 0.002936795 0.000800383
MIR-029a,c 0.003287862 0.003816043 0.000699254 0.029521487 0.013385748 0.034633338
MIR-029b-1,2 0.002261889 0.001620089 0.000255493 0.00018277 0.00454898
MIR-030a,e 0.015817568 0.008049749 0.009548634 0.009069786 0.025886829 0.024622819
MIR-030b 0.032213273 0.023990467 0.022876007 0.020156573 0.130639182 0.152129809
MIR-030c-1,2 0.041040641 0.029998695 0.02228078 0.016353471 0.064907638 0.101747572
MIR-030d 0.009544095 0.000165124 0.00596619 0.005911626
MIR-031-P 0.336204297 0.162341592 0.181180748 0.177014656 0.333619627 0.290619874
MIR-032 0.017677206 0.004104854 0.014120912 0.007388497 0.067239559 0.037292925
MIR-033 0.000324672 0.00036525 0.000309193 0.000780286 0.000264862 0.002796651
MIR-034a 0.011954566 0.009155803 0.01539088 0.055732849 0.028583929 0.041453444
MIR-034b 0.001773097 0.002599786 0.002959749 0.004140476 0.001218406 0.004212564
MIR-034c
MIR-092-1 0.000144635 0.00012471 0.000249382 0.005416338
MIR-092-2-P 0.010771247 0.005495992 0.007871286 0.006107824 0.026786559 0.04677749
MIR-093 0.012527037 0.015571612 0.025333462 0.025478784 0.040930343 0.045465145
MIR-095 0.000569694 0.000769415 0.001368625 0.00797047 0.003997888
MIR-096-P 1.124034307 0.278904587 0.891192854 1.147945555 1.097127024 1.409226265
MIR-098 0.259493277 0.221867471 0.150235855 0.319546152 0.729152542 0.6203603
MIR-099a 0.00173979 0.00092881 0.001507435 0.000970997 0.01062897 0.013378301
MIR-099b 0.053190985 0.052300573 0.348553029 0.60279369 0.087067166 0.111896186
MIR-100 0.008855046 0.012598498 0.006302979 0.025774187 0.074910178 0.064522774
Continued
154
Table A. continued
MIR-101-1 0.002931117 0.001015358 0.000221379 0.006172721
MIR-101-2 0.235941658 0.038637996 0.015768345 0.047561144 0.122494771 0.152216301
MIR-103-1,2 0.235761013 0.129790579 0.07850055 0.11851263 0.355137982 0.555116996
MIR-105-1,2 0.015572745 0.017640522 0.084224576 0.023321829 0.022313282 0.040999802
MIR-106a-P 0.001203728 0.002257931 0.002010854 0.0026566 0.002562772 0.001730264
MIR-106b 0.052692906 0.059722467 0.003582022 0.040712192 0.062722422 0.114530898
MIR-107 0.152248569 0.173840673 0.043072491 0.118730313 0.417790493 0.459355265
MIR-108 0.01075514 0.026687714 0.010867656 0.01581359 0.009152107 0.048140995
MIR-122a 0.002549942 0.00240095 0.002987762 0.002515619 0.00459102 0.011318596
MIR-124a-1,2,3 0.002686129 0.000658507 0.001218382 0.005805475 0.002438195 0.007479024
MIR-125a 0.084603905 0.048511667 0.036995332 0.044760803 0.098270457 0.083749203
MIR-125b-1 0.010984146 0.014991817 0.000781507 0.018714181 0.118832311 0.048184377
MIR-125b-2 0.001497081 0.000680202 0.006278051 0.001215792 0.006702658 0.006046269
MIR-126 0.045011644 0.078728542 0.002029374 0.035357859 0.109963571 0.167638075
MIR-127 1.336976465 1.067937391 1.102588584 1.241384226 1.586315318 2.386303875
MIR-128a 0.018793402 0.004390741 0.017214732 0.012338255 0.036454559 0.052427471
MIR-128b 0.012375542 0.006851913 0.006063076 0.015875042 0.017239928 0.036817826
MIR-129-2 0.206795267 0.112882799 0.216273539 0.22336642 0.270976861 0.553785149
MIR-130a-P 0.315547166 0.123233344 0.158971061 0.227245199 0.45856418 0.550764802
MIR-130b 0.000786065 0.001727677 0.006192231 0.010242067 0.001507291 0.005174768
MIR-132 0.000319802 0.005560879 0.00311748 0.016005766 0.013241228
MIR-133a-1,2 0.033429523 0.036249278 0.025803714 0.023672432 0.044246038 0.019377064
MIR-133b 0.050694641 0.044961176 0.089600499 0.072949466 0.049220413 0.185451876
MIR-134 0.154609575 0.079926214 0.025135864 0.047759407 0.200802491 0.349498161
MIR-135a-1,2 0.005116539 0.001398151 0.002868652 0.014636184 0.048358358 0.037962301
MIR-135b-P 0.015226372 0.002906444 0.003458274 0.002605853 0.007809707 0.031701486
MIR-136 0.000670647 0.003114314 0.001136085 0.001619897 0.027114306 0.011153206
MIR-137 0.000724876 0.000862998 0.001544331 0.002664959 0.008930174 0.023446165
MIR-138-1,2 0.001066134 0.000576575 0.001728739 0.007064216 0.003303705 0.003278719
MIR-139 0.082889667 0.066300087 0.144733578 0.132227093 0.070429984 0.272459041
MIR-140 0.000462177 0.00046819 0.000408068
MIR-141 0.011214605 0.014088019 0.005989983 0.006822167 0.024340432 0.048454846
MIR-142-P 0.517754671 0.26796966 0.479720857 0.526207683 0.243503948 0.543002321
MIR-143 0.008451526 0.015601852 0.010951802 0.039678663 0.026571787 0.044878267
MIR-144 0.000347188 0.000911752 0.0002132
MIR-145-P 0.023213737 0.011188544 0.022409909 0.020842934 0.137626297 0.085129164
MIR-146 0.00045774 0.00040188 0.000990003 0.000746375 0.000552401 0.000462866
MIR-147 0.00777064 0.002639743 0.001681868 0.009283466 0.030928658 0.049109324
MIR-148a-P 0.009808805 0.017715783 0.0377653 0.016374892 0.005185303 0.015497295
MIR-148b-P 0.018034323 0.004860362 0.011763634 0.006406296 0.075776119 0.017302158
MIR-149 0.122808776 0.047948868 0.088025179 0.095504546 0.195066247 0.356558393
MIR-150 0.030451003 0.012556238 0.023499357 0.018558678 0.03085871 0.059512592
MIR-151 0.003569036 0.003699959 0.004052687 0.005374349 0.015999433 0.023107056
MIR-152 0.002381058 0.001678749 0.001132278 0.014883908 0.008987076 0.027016784
MIR-153-1,2 0.004683363 0.003224637 0.010789898 0.002446533 0.019285657 0.024446526
MIR-154 0.035653711 0.001603305 0.00961808 0.00401225 0.004369722 0.046171047
Continued
155
Table A. continued
MIR-155 0.008739164 0.008826817 0.000401429 0.009368866 0.024332967 0.007784827
MIR-181a,c 0.006196471 0.01807232 0.000438938 0.004720704 0.066524961 0.06114737
MIR-181b-1,2 0.00207454 0.000549543 0.000796762 0.002495298 0.002561531 0.011229689
MIR-182 0.097172411 0.069783007 0.008536329 0.038550362 0.166420443 0.308155889
MIR-183 0.008598809 0.004633092 0.001991851 0.001918504 0.009382591 0.010075294
MIR-184 0.032143494 0.040467501 0.030947734 0.033263233 0.116268006 0.197394031
MIR-185 0.238603493 0.444582039 0.453059699 0.373060422 0.475669052 0.580944508
MIR-186-P 0.181171141 0.04262321 0.115565011 0.107269975 0.73587242 0.547239119
MIR-187 0.000558598 0.003961983 0.001154775 0.000310979 0.003765043 0.003647572
MIR-188b-P 0.007268549 0.00308475 0.001278001 0.000639631 0.008641111 0.02614141
MIR-190 0.000250425 0.000339337
MIR-191 0.000658371 0.000778382 0.000847187 0.000208854 0.000496219 0.002886264
MIR-192 0.018058502 0.013162306 0.041929765 0.019252308 0.020109466 0.033714331
MIR-193 0.008240885 0.015266213 0.010562928 0.003656862 0.010758286 0.014924788
MIR-194-1,2 0.004080247 0.000438034 0.001203061 0.002232689 0.004554802 0.004502761
MIR-195-P 0.297343011 0.24129101 0.262356887 0.110027703 0.21278363 0.799173843
MIR-196a-1 0.005299245 0.00660894 0.011725129 0.005306211 0.005740733 0.027134521
MIR-196a-2 0.007123347 0.001871324 0.00155058 0.005808471 0.015049184 0.049274832
MIR-196b 0.039444202 0.014298083 0.038965161 0.030449371 0.059391924 0.09970905
MIR-197 0.189617046 0.045936845 0.25367277 0.202323418 0.285322034 0.315722125
MIR-198 0.00087355 0.000214464 0.005640857 0.000440909 0.008943583 0.005461098
MIR-199a-1-P 0.497798079 0.799643061 1.013671412 0.731949855 1.000941091 1.476407805
MIR-199a-2-P 0.013458119 0.005413726 0.007984001 0.003251728 0.006032081 0.039974679
MIR-199b-P 0.180422175 0.075547984 0.100902272 0.069749961 0.316475576 0.3840639
MIR-200a-P 4.095998166 1.74546759 5.53811714 6.880177186 6.191369533 7.367462281
MIR-200b 0.021707706 0.015252273 0.107896433 0.01228045 0.068796647 0.176803013
MIR-200c 0.007691571 0.002094369 0.001304947 0.001843116 0.03170013 0.064013712
MIR-203 0.001020997 0.001314431 0.006133442 0.004690611 0.001887462
MIR-204 0.107210794 0.041991222 0.05457712 0.031980764 0.225873209 0.312754006
MIR-205 0.013931497 0.005608937 0.000388849 0.001429942 0.006043995 0.003970808
MIR-206 0.009027684 0.001421654 0.0012271 0.002029891 0.017358109 0.018082159
MIR-208 0.003284983 0.002320861 0.004636049 0.00870919 0.011147898 0.026583793
MIR-210 1.139689297 0.685260089 0.415676205 0.167266765 1.354844773 2.194505323
MIR-211 0.263884875 0.182771252 0.27665647 0.209902568 0.446570721 0.78599349
MIR-212 0.026633901 0.001685785 0.023479583 0.058080864 0.185966101 0.106532776
MIR-213 0.007426131 0.005707231 0.006498503 0.004705345 0.034337851 0.010997419
MIR-214 0.142593346 0.070352869 0.157445297 0.138599717 0.223581166 0.318789666
MIR-215 0.005906295 0.00176932 0.005884178 0.000159815 0.000374749 0.002932552
MIR-216 0.026532769 0.015560099 0.018389264 0.010548109 0.272845768 0.08897441
MIR-217 0.000544327 0.000145199 0.001535443 0.000902913
MIR-218-1,2 0.019397413 0.007089284 0.004667189 0.008428845 0.027820923 0.042569554
MIR-219-1,2 0.0102539 0.002822347 0.001696013 0.000178188 0.000853387 0.013578028
MIR-220 0.004515206 0.004346103 0.001716496 0.005808445 0.003181942 0.005308315
MIR-221 0.001690099 0.001967066 0.000540588 0.008587802 0.010153596 0.001787793
MIR-222-P 0.003724321 0.002775237 0.001366649 0.00138896 0.007657905 0.009410791
MIR-223 0.001013411 0.001732987 0.001290372 0.000699716 0.002335613 0.005021617
Continued
156
Table A. continued
MIR-224 0.021638234 0.025557421 0.038613913 0.038797506 0.008696566 0.033478084
MIR-296 0.016779332 0.013666604 0.014778494 0.009389338 0.019221091 0.031866696
MIR-299-P 0.030739523 0.030973593 0.090631324 0.087728184 0.095289071 0.148039212
MIR-301 0.002905409 0.001421795 0.00102095 0.000386695 0.0058713 0.017762067
MIR-302 0.001671448 0.001432421 0.001484567 0.001344435 0.008632556 0.005327614
MIR-302b 0.014037692 0.009199927 0.008301893 0.005957663 0.037275488 0.0353411
MIR-302c 0.006637214 0.002553558 0.002007514 0.005042276 0.000953833 0.007174643
MIR-302d 0.003558781 0.003707237 0.004372718 0.004119177 0.005444761 0.007732396
MIR-320 0.011101828 0.005988764 0.019042432 0.00640881 0.024686944 0.043652157
MIR-321 1.795608797 3.289936115 0.258181596 2.148871508 2.323744691 1.960181534
MIR-323 0.039524501 0.041433172 0.034394831 0.039717296 0.055565514 0.116587722
MIR-324-P 0.055726412 0.04804315 0.030020869 0.0252393 0.078389518 0.08321952
MIR-325 0.01259103 0.033482303 0.037368606 0.039328888 0.017826522 0.048511001
MIR-326-P 0.001893571 0.001223979 0.000421041 0.001382688 0.002858711 0.005407891
MIR-328 0.206092666 0.230141182 0.791047411 0.437211793 0.594908654 0.367686795
MIR-330 0.03223628 0.012095126 0.016349525 0.009695901 0.036549338 0.043044889
MIR-331-P 0.097557228 0.027971493 0.285186297 0.051885698 0.228247912 0.521268566
MIR-335 0.001043954 0.001386457 0.000729032
MIR-337 0.037751652 0.022216896 0.043250978 0.038542547 0.054823702 0.046272209
MIR-338 0.000773837 0.063324521 0.036599309 0.000418386
MIR-339 0.073799051 0.036187345 0.055842083 0.069319628 0.088011117 0.12692822
MIR-340 0.062018312 0.041251863 0.041818353 0.029906393 0.117032576 0.127060479
MIR-342 0.01350495 0.007849076 0.014434516 0.020664084 0.0403018 0.032895494
MIR-345 0.350543544 0.270729043 0.389454197 0.313538763 0.153215809 0.595157028
MIR-346 0.090088862 0.015340152 0.567170369 0.581581474 0.130991406 0.249313648
MIR-361 0.020562066 0.013229232 0.014924264 0.017117861 0.024979737 0.030476389
MIR-367 0.002762393 0.000297385 0.003626158 0.00432079
MIR-368 0.008678889 0.002211545 0.000533164
MIR-369 0.011705903 0.014161332
MIR-370 0.061321384 0.024106651 0.029574387 0.04990111 0.100154009 0.122309152
MIR-371 0.078235737 0.04891952 0.06485547 0.057243878 0.124162986 0.151275658
MIR-372 0.000571339 0.000329452 0.000385333 0.001134313 0.000248125 0.002027938
MIR-373 0.009413654 0.009162396 0.00822495 0.012185728 0.006167551 0.023381978
MIR-374-P 0.001585969 0.000821807
MIR-375 0.113815509 0.012963212 0.065761001 0.019541117 0.181389276 0.235983807
MIR-376 0.007708886 0.001442693 0.00042116 0.000414878 0.167537455 0.2948857
MIR-377 0.003676877 0.002957325 0.005718306 0.01205171 0.015430767 0.024634197
MIR-378 0.001544141 0.003236343 0.008925366 0.004419913 0.009333139 0.010166908
MIR-379 0.023621478 0.01855919 0.00534838 0.024262116 0.059573708 0.062262282
MIR-380 0.011458405 0.005951694 0.008073218 0.006201162 0.015381922 0.003542559
MIR-381 0.003291062 0.002320368 0.001581146 0.000445611 0.007160823 0.002066094
MIR-382 0.003515956 0.00153345 0.001235961 0.000448333 0.004727747 0.002053751
MIR-383 0.006798745 0.002960483 0.002885863 0.0046032 0.008793981 0.012559425
MIR-384 0.000510172 0.000186446
MIR-422b 0.021293355 0.012380952 0.032601396 0.036130655 0.037434027 0.053077152
MIR-423 0.015180115 0.01248593 0.015330011 0.012016893 0.008449895 0.025349617
Continued
157
Table A. continued
MIR-424 0.000655915 0.00138276 0.00039961 0.000268085 0.038575471 0.025073562
MIR-425 0.005043245 0.007785204
18S rRNA (CT) 7.0593355 7.1759525 7.066974 7.09703565 7.430182 8.0021922
Tissue Type Adjacent Benign
miRNAs OUP28 (B) OUP48 (B) OUP91 (B) OUP27 (B) OUP33 (B) OUP42 (B)
let-7a-1* 0.000234982 0.000164582
let-7a-2-P 0.08413606 0.043721617 0.037881836 0.321861154 0.070628189 0.07727867
let-7a-3* 0.008107015 0.004695749 0.009361647 0.011628666 0.000224623 0.005076983
let-7b* 0.0039822 0.005228008 0.006809922 0.034445095 0.001812017 0.01721141
let-7c* 0.000145887 0.00286499
let-7d* 0.008425529 0.013956868 0.014672678 0.083597892 0.013210459 0.019800505
let-7e* 0.001577992 0.001475721
let-7f-1* 0.015723526 0.027090448 0.041635734 0.117425844 0.007365754 0.04703942
let-7f-2* 0.002114508 0.003559086 0.003177816
let-7g* 0.005819238 0.005261778 0.011710622 0.048761062 0.003512886 0.010525949
let-7i* 0.048058871 0.005084697 0.008521305 0.007224064 0.019896356
MIR-001-1 0.012619793 0.002494582 0.002278975 0.00800549 0.00740626 0.005949032
MIR-001-2 0.065555906 0.002539466 0.0036048 0.061903482 0.056976879 0.003923197
MIR-007-1 0.014817954 0.013399478 0.006481258 0.026311383 0.017638105 0.005078746
MIR-007-2 0.004043715 0.000432792 0.003231624 0.004259125 0.00090441
MIR-007-3 0.212521554 0.198384961 0.404523822 0.850770178 0.119152689 0.233273943
MIR-009-1-P 0.276967782 0.075619943 0.061080305 0.077312895 0.295916679 0.134810995
MIR-009-2-P 0.156779308 0.081782307 0.028972552 0.064461111 0.211529667 0.09337794
MIR-009-3-P 0.001808542 0.001189292 0.002079533 0.001513423
MIR-010a,b 0.003091333 0.004893302 0.033574759 0.000295203
MIR-015a 0.027070532 0.011626463 0.004110325 0.047280361 0.02193097 0.004886728
MIR-015b 0.011300818 0.01484316 0.0686602 0.000186049 0.065753139
MIR-016-1 0.06533433 0.037405652 0.011899783 0.168712511 0.045605809 0.034755559
MIR-016-2 0.305141449 0.121720711 0.039833597 0.109591808 0.237746403 0.065151562
MIR-017-P 0.045966307 0.052137368 0.029531134 0.025978931 0.094595766 0.044719568
MIR-018 0.397378595 0.592319944 0.403941442 0.382361685 0.189655934 0.307391797
MIR-019a-P 0.070918659 0.105428847 0.059611338 0.059209557 0.016635539 0.072384598
MIR-019b-1-P 0.054198137 0.018827661 0.01244431 0.125487574 0.015128786
MIR-019b-2-P 0.000501627 0.000846554
MIR-020 0.000402039 0.000705469 0.000370353 0.000518267
MIR-021 3.922993838 3.365094616 3.227823223 11.79674446 1.651297909 2.252012085
MIR-022-P 0.019673918 0.036212487 0.024622304 0.051963192 0.115946439 0.053735249
MIR-023a 0.059530985 0.020981463 0.034731657 0.131601594 0.068897857 0.023021451
MIR-023b-P 0.084095538 0.079623719 0.033565247 0.106167041 0.160887196 0.063663998
MIR-024-1,2 3.509217989 2.809710656 2.701301307 6.696340409 4.346802115 3.804182791
MIR-025 0.035892832 0.010981383 0.014457046 0.090961627 0.019237164 0.023804845
MIR-026a-1,2 0.010089406 0.008074027 0.00606708 0.01501254 0.018548707 0.010727663
MIR-026b 0.19202061 0.099960302 0.084238997 0.078715517 0.163247683 0.095625359
MIR-027a, b 0.03166631 0.025928617 0.016429884 0.128196108 0.101167508 0.066751384
MIR-028 0.004084006 0.001063208 0.001473975 0.005803773 0.001906219 0.004740957
Continued
158
Table A. continued
MIR-029a,c 0.015314654 0.004782879 0.104714045 0.007100252 0.028025503
MIR-029b-1,2 0.000237819 0.000533374 0.000627306 0.000680682
MIR-030a,e 0.013500878 0.00714361 0.006099316 0.018799139 0.035800273 0.015091699
MIR-030b 0.102604511 0.057823994 0.067999199 0.095296258 0.096918267 0.104093152
MIR-030c-1,2 0.084763359 0.025625857 0.029109605 0.08391278 0.0280025 0.024250731
MIR-030d 0.000629274 0.008994885 0.008634738 0.028996616 0.023877911 0.008541855
MIR-031-P 0.309218828 0.131902706 0.110714 0.077957567 0.09198264 0.139425425
MIR-032 0.020608954 0.028395794 0.003922288 0.030303314 0.009475097 0.005815605
MIR-033 0.000220521 0.000527277 0.000727156
MIR-034a 0.024957679 0.014539687 0.001610452 0.048682375 0.053107279 0.012241037
MIR-034b 0.016092113 0.00132246 0.000597503 0.000946134 0.007038862 0.00072473
MIR-034c
MIR-092-1 0.000255167 0.002282102 0.004221994 0.001558821
MIR-092-2-P 0.025387419 0.002898233 0.00448304 0.010784472 0.011201901 0.005442254
MIR-093 0.06071983 0.017685172 0.004436942 0.012888399 0.034225286 0.054579692
MIR-095 0.006051509 0.002562215 0.000204695 0.020968528 0.004869382 0.00991631
MIR-096-P 0.779840275 0.124493349 0.216903926 0.111881536 0.793870955 0.528221551
MIR-098 0.83784546 0.41963199 0.239425392 1.672631188 0.579853727 0.664410634
MIR-099a 0.002226261 0.00306157 0.002911853 0.006143723 0.001031319 0.000712835
MIR-099b 0.108884694 0.094329483 0.070551256 0.162735778 0.112613947 0.0792654
MIR-100 0.095282665 0.027529354 0.022930348 0.384194792 0.030445365 0.027402372
MIR-101-1 0.003438277 0.00037762 0.000962309 0.001052697 0.002080896
MIR-101-2 0.168955834 0.154783551 0.346808117 0.14476722 0.089292228 0.109907411
MIR-103-1,2 0.294929506 0.227929185 0.152352974 0.365027704 0.252951802 0.355640509
MIR-105-1,2 0.03104887 0.029861492 0.010025584 0.008893589 0.077070626 0.036737253
MIR-106a-P 0.000796113 0.000331205 0.00121592 0.004037087 0.00345913 0.000291003
MIR-106b 0.077773343 0.029381494 0.047628917 0.10624953 0.040860266 0.0639964
MIR-107 0.365844904 0.206893406 0.192343245 1.063596452 0.267694596 0.41292311
MIR-108 0.018157628 0.011169745 0.002391069 0.001568711 0.040712706 0.01670109
MIR-122a 0.004642035 0.000196141 0.001944131 0.001203634
MIR-124a-1,2,3 0.008884637 0.001205011 0.000572846 0.00659248 0.004228399
MIR-125a 0.077360213 0.063626963 0.02803547 0.065445208 0.030932139 0.042160859
MIR-125b-1 0.078756842 0.010662363 0.009916564 0.230666015 0.020745646 0.024832014
MIR-125b-2 0.002370775 0.003722611 0.000243287 0.005118509 0.000501884
MIR-126 0.102293478 0.011745293 0.016270322 0.232877049 0.027331897 0.037173892
MIR-127 2.216024097 1.656945407 1.617585051 1.082989585 1.126158131 0.83118589
MIR-128a 0.028906577 0.011604668 0.008562469 0.03655326 0.015758788 0.027933401
MIR-128b 0.012189786 0.011882285 0.007685384 0.00596623 0.008038304 0.004625216
MIR-129-2 0.313967486 0.173418542 0.05710853 0.025112429 0.560911831 0.245182101
MIR-130a-P 0.40428962 0.143096676 0.060107218 0.04265066 0.436634012 0.204870912
MIR-130b 0.028034916 0.00558854 0.000374729 0.010806618 0.000745937
MIR-132 0.003088596 0.008384841 0.005825663 0.032162545 0.00081037 0.000807703
MIR-133a-1,2 0.045509863 0.021765222 0.015713736 0.125734622 0.042394265 0.02670025
MIR-133b 0.073986767 0.056522578 0.013035905 0.009255684 0.062635393 0.062626327
MIR-134 0.22107918 0.089114319 0.034670863 0.019632366 0.193212913 0.068271183
MIR-135a-1,2 0.029853483 0.026907759 0.00408374 0.023525976 0.008821905 0.00515723
Continued
159
Table A. continued
MIR-135b-P 0.011710744 0.000449021 0.008062137 0.006282678 0.017146339 0.001666304
MIR-136 0.012992898 0.00315236 0.019444508 0.028983394 0.011324643 0.004839458
MIR-137 0.004923941 0.000762835 0.010697556 0.001176041 0.002353161 0.000265256
MIR-138-1,2 0.000718863 0.001660083 0.000749769 0.002953181 0.001111941 0.003175957
MIR-139 0.119689921 0.043784334 0.040409269 0.006288985 0.121668062 0.060761659
MIR-140 0.000234661
MIR-141 0.031089281 0.017324218 0.017328728 0.006608451 0.017621022 0.004804847
MIR-142-P 0.210736785 0.026621858 0.010171153 0.060264945 0.021759775 0.064671419
MIR-143 0.010665412 0.009474773 0.008380111 0.032199461 0.003236343 0.010908009
MIR-144 0.00042125 0.000182078 0.000278555
MIR-145-P 0.061210505 0.019807959 0.017214213 0.490124228 0.027306456 0.035988046
MIR-146 0.000256347 0.000264944
MIR-147 0.02497475 0.009668864 0.002814281 0.001669416 0.045892586 0.024778816
MIR-148a-P 0.007124599 0.006653592 0.009418892 0.003325934 0.007954596 0.004778285
MIR-148b-P 0.042273183 0.006743095 0.017285176 0.093089604 0.064617105 0.045206955
MIR-149 0.132515275 0.166105594 0.053524494 0.018059921 0.246809173 0.144175814
MIR-150 0.040269282 0.02143109 0.010986583 0.095748014 0.049614493 0.030865641
MIR-151 0.012811605 0.01495816 0.002590492 0.001253338 0.014571502 0.008452156
MIR-152 0.010489612 0.013312184 0.000643859 0.022770052 0.004651357 0.00018941
MIR-153-1,2 0.006999621 0.011451057 0.020678761 0.01088628 0.017671311 0.00160908
MIR-154 0.000473873 0.010814262 0.042956584 0.054559846 0.000850523 0.019621213
MIR-155 0.00782965 0.003305353 0.000680438 0.209116219 0.00161709 0.001200658
MIR-181a,c 0.031671194 0.028904884 0.016081654 0.097962498 0.032485651 0.048816801
MIR-181b-1,2 0.003025911 0.00132746 0.002040976 0.00591001 0.008911676 0.001499668
MIR-182 0.231623906 0.140235992 0.141192667 0.031469223 0.172687782 0.143819337
MIR-183 0.007699829 0.008899463 0.001471681 0.00422692 0.010514973 0.016087992
MIR-184 0.131239651 0.084854829 0.001509601 0.019808252 0.115810496 0.05080015
MIR-185 0.6120577 0.350467257 0.283879597 0.506095436 0.537428196 0.365881422
MIR-186-P 0.392152056 0.186860634 0.263269641 0.643156559 0.476374442 0.365849596
MIR-187 0.005301438 0.000484393 0.000333395 0.008131597 0.005681169
MIR-188b-P 0.014227378 0.001181555 0.001280577 0.006393178 0.002498412
MIR-190 0.000151625 0.000233282 0.000280636
MIR-191 0.000558084 0.000465288 0.000249152
MIR-192 0.044483283 0.021902465 0.008314156 0.001383875 0.017967624 0.007758328
MIR-193 0.01844468 0.00329149 0.000616123 0.013183323 0.011183448 0.002291398
MIR-194-1,2 0.003320436 0.0006792 0.001073062 0.000821191 0.002687984 0.002336058
MIR-195-P 0.492637743 0.172730717 0.171225273 0.060741796 0.323705643 0.224219797
MIR-196a-1 0.004301889 0.007242847 0.000641151 0.005008573 0.02227901 0.018257184
MIR-196a-2 0.029827711 0.012476042 0.000687665 0.012590125 0.014437161 0.001411738
MIR-196b 0.048395579 0.015876383 0.008424198 0.046986866 0.038942503 0.010778488
MIR-197 0.25833653 0.078302331 0.077371233 0.073212833 0.275926977 0.134609121
MIR-198 0.008228191 0.000691307 0.000310343 0.013556254 0.000831424 0.000742233
MIR-199a-1-P 1.067870771 0.368490561 0.259500922 0.182511765 0.674776866 0.366588407
MIR-199a-2-P 0.033527555 0.006555216 0.007164407 0.16130291 0.024979933 0.039653598
MIR-199b-P 0.255282062 0.101079131 0.038226186 0.025767125 0.220654247 0.072754489
MIR-200a-P 4.703462067 2.384878171 1.777765542 0.657466991 5.951620014 3.004357655
Continued
160
Table A. continued
MIR-200b 0.072216788 0.190434275 0.031083937 0.003258583 0.181465717 0.049982663
MIR-200c 0.039647565 0.066019456 0.008855369 0.018427388 0.099660451 0.014937821
MIR-203 0.00406014 0.000479102 0.000983115
MIR-204 0.165458472 0.051715716 0.027832283 0.006131148 0.144945296 0.037148418
MIR-205 0.002896328 0.000585291 0.000207173 0.017841928 0.006921418 0.003178783
MIR-206 0.008818323 0.024298466 0.001241969 0.00072871 0.012548232 0.004020759
MIR-208 0.017574587 0.003567121 0.009921532 0.009112556 0.000879958
MIR-210 1.90050401 0.534722233 0.508250653 0.05847618 1.154804728 0.404474756
MIR-211 0.570812 0.292701404 0.153841866 0.129316061 0.593116709 0.273940707
MIR-212 0.132632898 0.082847161 0.124400791 0.578056481 0.069205496 0.062857494
MIR-213 0.030048975 0.011276828 0.01340049 0.047280738 0.029589998 0.007572391
MIR-214 0.258099557 0.07755317 0.110557597 0.089510974 0.158627567 0.113021102
MIR-215 0.009568285 0.007101194 0.006205621
MIR-216 0.107962865 0.104393086 0.088923656 0.001513805 0.07325771 0.074498752
MIR-217 0.00040942 0.001661952 0.000245461 0.000849568 0.000603338
MIR-218-1,2 0.05234393 0.004554355 0.013413198 0.057306153 0.010000916 0.006047498
MIR-219-1,2 0.005928826 0.001038774 0.001767721 0.009114754 0.01049447
MIR-220 0.006214304 0.005444088 0.000435066 0.009085524 0.005951004 0.002214173
MIR-221 0.045575682 0.011350838 0.002001654 0.055376008 0.01520112 0.000667747
MIR-222-P 0.016091996 0.008175902 0.001190568 0.019668917 0.00567379 0.000675161
MIR-223 0.002923861 0.00245088 0.000530116 0.003006313 0.003730473 0.001107651
MIR-224 0.043112576 0.014976456 0.013955886 0.00855908 0.032614593 0.014438904
MIR-296 0.01532531 0.004319816 0.002994118 0.048733693 0.028180781 0.009503203
MIR-299-P 0.078681325 0.054944774 0.003418628 0.010905762 0.117998949 0.050009087
MIR-301 0.014894015 0.029149846 0.002720049 0.054720316 0.027557903 0.023711744
MIR-302 0.003014402 0.001285655 0.004369301 0.014803005 0.006631148 0.001801872
MIR-302b 0.029120594 0.014553047 0.018129005 0.005290135 0.009473156 0.010280506
MIR-302c 0.01037688 0.001964958 0.000777434 0.001917303 0.006566469 0.005096544
MIR-302d 0.00483927 0.003929107 0.000257331 0.000770693 0.003552945 0.001754567
MIR-320 0.019564548 0.01396628 0.005496657 0.005817255 0.034933655 0.007863891
MIR-321 1.634714926 0.989010381 0.942101933 1.525698475 0.848276452 1.278434786
MIR-323 0.086797637 0.058084703 0.053378888 0.026394833 0.066951023 0.06927519
MIR-324-P 0.052501377 0.03753091 0.013700383 0.017316442 0.037078056 0.031599785
MIR-325 0.027058498 0.00847426 0.010734204 0.009581966 0.034536769 0.0164059
MIR-326-P 0.002971626 0.00247575 0.001537659 0.000482912 0.00297898 0.001623585
MIR-328 0.392702332 0.446743012 0.164869229 0.03598269 0.92444021 1.566199583
MIR-330 0.030269435 0.009721766 0.003750032 0.002039085 0.015856096 0.003701896
MIR-331-P 0.022495256 0.174547073 0.044244489 0.013797755 0.392531114 0.149622295
MIR-335 0.000464215 0.000294875
MIR-337 0.080553862 0.015869979 0.00096346 0.001705795 0.044751254 0.011896261
MIR-338 0.000598119 0.008156783 0.006396992 0.139155846 0.045999853
MIR-339 0.123387664 0.05465162 0.058363353 0.060271232 0.071044162 0.05604345
MIR-340 0.06950117 0.064959826 0.088047879 0.149203974 0.03361564 0.1144591
MIR-342 0.022062706 0.005099657 0.002649976 0.072531544 0.023778218 0.012693529
MIR-345 0.210104515 0.191396794 0.088861547 0.016947384 0.289629994 0.166878334
MIR-346 0.199630432 0.285835511 0.22105382 0.298317469 0.174906695 0.119635509
Continued
161
Table A. continued
MIR-361 0.029099871 0.011969806 0.01483849 0.014552788 0.030087383 0.015790116
MIR-367 0.006116846 0.002986056 0.00770823 0.001278695
MIR-368 0.000188323 0.005916756 0.002077999 0.000558584 0.00019956
MIR-369 0.00915447 0.0063059 0.00872308 0.01042334 0.013383739
MIR-370 0.043934987 0.06120535 0.059649571 0.084327639 0.037748769 0.045432439
MIR-371 0.125301281 0.03285388 0.005353871 0.006172574 0.126983629 0.034278792
MIR-372 0.000701899 0.002392081
MIR-373 0.014851918 0.008239669 0.000776753 0.000501465 0.015317641 0.004096831
MIR-374-P 0.000461169 0.002166815 0.000859515 0.002013852
MIR-375 0.161824457 0.104045687 0.171561361 0.003519514 0.071583774 0.120343704
MIR-376 0.070940562 0.102342307 0.144028734 0.385698214 0.058887467 0.120841681
MIR-377 0.008541343 0.002637997 0.009593727 0.015221854 0.034747366 0.003542248
MIR-378 0.005377037 0.001527965 0.002291028 0.000365252 0.019686459 0.002171276
MIR-379 0.008220296 0.054211926 0.066550835 0.102617122 0.00901298 0.063004604
MIR-380 0.015409059 0.008523006 0.029427838 0.001436367 0.010491524
MIR-381 0.000764528 0.001041043 0.000234227 0.003273774 0.003442245
MIR-382 0.001563587 0.00132686 0.004607718 0.002593456 0.004123975 0.002206109
MIR-383 0.008034253 0.009597698 0.001416751 0.00076871 0.011731058 0.001394468
MIR-384 0.000638454
MIR-422b 0.042041926 0.019073112 0.023588534 0.000355638 0.023065069 0.007148073
MIR-423 0.020461469 0.007385471 0.003011961 0.000931051 0.012388484 0.004602476
MIR-424 0.015747756 0.02244214 0.00022913 0.02186877 0.002311079 0.011818208
MIR-425 0.004937966 0.004139409
18S rRNA (CT) 7.288695 7.2963655 7.3134995 7.9507668 7.22896885 7.6139735
Tissue Type Adjacent Benign
miRNAs OUP105 (B) OUP122 (B) OUP139 (B) OUP29(B) OUP49(B) OUP101(B)
let-7a-1* 0.002036074 0.003450972 0.005977055
let-7a-2-P 0.171442693 0.271995421 0.041441454 0.059309784 0.03991665 0.0373892
let-7a-3* 0.005822275 0.011321189 0.003395118 0.002460471 0.001943705 0.013502889
let-7b* 0.034222971 0.012946377 0.015466166 0.015948394 0.005586341 0.029624311
let-7c* 0.000671359 0.000450217 0.00053108 0.000608686
let-7d* 0.100665297 0.025669176 0.020641819 0.020184014 0.019825245 0.029853645
let-7e* 0.001469486 0.000861823
let-7f-1* 0.142972622 0.063836407 0.038071334 0.042773303 0.031752422 0.031526819
let-7f-2* 0.00293584 0.002239283 0.001844438 0.003533604
let-7g* 0.046798361 0.024640883 0.007943425 0.014480773 0.019393392 0.008377804
let-7i* 0.036662622 0.017135076 0.022833961 0.016913387
MIR-001-1 0.004958847 0.0001661 0.002190754 0.01204077 0.00045796 0.009270089
MIR-001-2 0.050465974 0.070586582 0.003562829 0.073032626 0.06957549 0.026205808
MIR-007-1 0.052003348 0.024267584 0.017542497 0.02222934 0.053522895 0.003160654
MIR-007-2 0.005175238 0.008434637 0.000779341 0.000793416 0.002347539 0.000301137
MIR-007-3 0.833719983 1.115347113 0.14052799 0.273927862 0.182279026 0.004832704
MIR-009-1-P 0.134446706 0.060378816 0.113434541 0.122419596 0.121941531 0.269762725
MIR-009-2-P 0.167261501 0.097165285 0.101194404 0.085711727 0.087539926 0.083538869
MIR-009-3-P 0.004037377 0.000179446 0.003604225 0.000417506
Continued
162
Table A. continued
MIR-010a,b 0.008907423 0.004366331 0.000205852 0.000409696 0.022055725
MIR-015a 0.014350366 0.03057341 0.006486835 0.014238485 0.015435339 0.016705841
MIR-015b 0.008878821 0.009736509 0.007673561 0.002112287 0.017943796 0.023917169
MIR-016-1 0.058628454 0.139459756 0.054333674 0.03133548 0.081828231 0.033140827
MIR-016-2 0.139998892 0.018137724 0.162999554 0.080853269 0.145360881 0.340954332
MIR-017-P 0.021996798 0.050395694 0.038492306 0.041174756 0.032552045 0.07303212
MIR-018 0.863942271 0.829451062 0.181720542 0.315043821 0.407389999 0.081603504
MIR-019a-P 0.126659818 0.059801616 0.053923518 0.065642087 0.022363014 0.038385797
MIR-019b-1-P 0.012807377 0.007224464 0.024924285 0.032861165 0.006048638 0.017852921
MIR-019b-2-P 0.00039806
MIR-020 0.000735194 0.00738248 0.000673233 0.000191459 0.001694727 0.00040318
MIR-021 11.41062428 17.49711634 0.710014267 1.841671078 8.281381564 0.580710256
MIR-022-P 0.074296616 0.112934663 0.032338441 0.032673151 0.014816192 0.074062801
MIR-023a 0.143565133 0.178679901 0.026684317 0.059395087 0.034245292 0.053596289
MIR-023b-P 0.137688485 0.075503575 0.104605848 0.058129948 0.011766993 0.148822382
MIR-024-1,2 6.061510365 8.819499397 2.586544333 3.085846757 2.973793498 1.789123428
MIR-025 0.079524341 0.032275623 0.017101799 0.011242997 0.0122648 0.020627588
MIR-026a-1,2 0.006972902 0.022133889 0.014824789 0.008222472 0.02057332 0.005486999
MIR-026b 0.106487598 0.041007689 0.126855583 0.106156964 0.128376004 0.1483895
MIR-027a, b 0.099868625 0.248310258 0.040927105 0.033746844 0.03978576 0.073597033
MIR-028 0.004212822 0.004134382 0.000885828 0.002317432 0.001789441 0.001631792
MIR-029a,c 0.072507011 0.12539383 0.00055959 0.002499034 0.036865751 0.004236411
MIR-029b-1,2 0.0004825 0.000227837 0.000332737 0.000438608 0.00035349
MIR-030a,e 0.012676992 0.017232308 0.010349837 0.026180545 0.026286584 0.049497618
MIR-030b 0.08675227 0.065113354 0.044868505 0.072402806 0.062383507 0.028530432
MIR-030c-1,2 0.05395808 0.046819465 0.030936492 0.021233447 0.044577993 0.01898766
MIR-030d 0.009111071 0.027173068 0.017276035 0.00751518 0.00466896 0.02339306
MIR-031-P 0.177638207 0.079189023 0.137546985 0.157096878 0.133362778 0.137182696
MIR-032 0.038293645 0.031802396 0.02579764 0.031319346 0.020035301 0.030698148
MIR-033 0.000226292 0.00037556 0.000268967 0.001281948
MIR-034a 0.026388393 0.019198052 0.014307143 0.013880409 0.012774224 0.002883053
MIR-034b 0.001978356 0.000367248 0.003202699 0.001360882 0.000767622 0.004085926
MIR-034c
MIR-092-1 0.003350196 0.004855447 0.001256448
MIR-092-2-P 0.001062677 0.005118988 0.007529355 0.002016076 0.001410371 0.013147952
MIR-093 0.065061835 0.02208181 0.012338928 0.006579795 0.024184415 0.014505315
MIR-095 0.012980536 0.010569436 0.003325673 0.003332236 0.001801792 0.011950369
MIR-096-P 0.289410731 0.062771642 0.454660135 0.457445228 0.538086669 0.307379087
MIR-098 1.854886382 1.719247526 0.52460553 0.532373225 0.90276192 0.370778866
MIR-099a 0.00842044 0.010464631 0.004092118 0.012038921 0.004242218 0.005254097
MIR-099b 0.198619176 0.288660432 0.108264219 0.360399251 0.304262401 0.229880886
MIR-100 0.194540821 0.365647846 0.030455739 0.048996718 0.059078744 0.009407603
MIR-101-1 0.004468616 0.002071022 0.000813845 0.000600037 0.000311706 0.003589417
MIR-101-2 0.137637531 0.097090959 0.03202479 0.065109322 0.098992084 0.11285032
MIR-103-1,2 0.635003223 0.504839524 0.161954832 0.240952541 0.855815652 0.119736833
MIR-105-1,2 0.006915229 0.005985592 0.02664507 0.026595517 0.009716743 0.04703591
Continued
163
Table A. continued
MIR-106a-P 0.001869572 0.001034073 0.001365182 0.000648165 0.00068566
MIR-106b 0.151382078 0.123441146 0.038005958 0.067512662 0.15699271 0.031692185
MIR-107 0.849026106 1.000927701 0.201687408 0.270442898 1.250803334 0.097332061
MIR-108 0.022524616 0.012384018 0.00882707 0.014676039 0.010268555 0.032439759
MIR-122a 0.00410661 0.000117169 0.003006461 0.000874917 0.001824824
MIR-124a-1,2,3 0.00118559 0.00099952 0.00114563 0.006629864
MIR-125a 0.059035122 0.057205821 0.020948125 0.070753554 0.025069647 0.138175134
MIR-125b-1 0.212750263 0.653496887 0.029321636 0.036891862 0.006024885 0.014074963
MIR-125b-2 0.009814142 0.004275673 0.00231327 0.005253252 0.010791378 0.000616116
MIR-126 0.212553632 0.179647874 0.053214968 0.049590254 0.085211766 0.018958044
MIR-127 1.580111997 0.804091882 2.806568787 1.726232656 1.829027757 1.874928941
MIR-128a 0.078699093 0.049085689 0.023713089 0.023060761 0.03865002 0.034235346
MIR-128b 0.007774784 0.002056656 0.011013169 0.00801294 0.009642236 0.004853733
MIR-129-2 0.148825054 0.048189838 0.190066532 0.201090582 0.17273316 0.198988908
MIR-130a-P 0.118573155 0.032977247 0.17259211 0.221321486 0.136246821 0.223315906
MIR-130b 0.002067666 0.005265759 0.002967465 0.010841299 0.002119046 0.006357183
MIR-132 0.022289334 0.139400367 0.001605969 0.016497771 0.000713527
MIR-133a-1,2 0.048650816 0.015192017 0.026170729 0.043770113 0.033140897 0.027355418
MIR-133b 0.012227238 0.004156224 0.046629909 0.05811623 0.041400528 0.03691949
MIR-134 0.11129609 0.071201319 0.105675176 0.076795944 0.05727028 0.116529725
MIR-135a-1,2 0.047675035 0.110184735 0.003295847 0.013291686 0.046775665 0.000967633
MIR-135b-P 0.00223109 0.001077774 0.003528515 0.005737872 0.00987869 0.005730525
MIR-136 0.031321949 0.046587566 0.005894705 0.00387682 0.002493754 0.016141936
MIR-137 0.002091926 0.000873836 0.000987531 0.001641519 0.00455792 0.00225232
MIR-138-1,2 0.000261425 0.000176663 0.002243003 0.000862323 0.000912428 0.002025076
MIR-139 0.023772341 0.007196872 0.062234157 0.115061367 0.095064564 0.103355704
MIR-140 0.000167232
MIR-141 0.009618155 0.009466634 0.011467498 0.015464295 0.016372018 0.006790775
MIR-142-P 0.039220883 0.023985682 0.0859142 0.045270166 0.033048073 0.119431344
MIR-143 0.022120163 0.041179932 0.007770615 0.013418144 0.059485216 0.006969916
MIR-144 0.000166561 0.000211268
MIR-145-P 0.466023728 0.327857606 0.021918235 0.049873592 0.513378566 0.024421607
MIR-146 0.000411582 0.000295193 0.00024814 0.000453244
MIR-147 0.024019065 0.00789972 0.00916992 0.007857938 0.003014966 0.012075296
MIR-148a-P 0.005289774 0.00262788 0.009238757 0.013823655 0.008645337 0.005668697
MIR-148b-P 0.108909961 0.036040423 0.058186727 0.050351233 0.016842685 0.039522621
MIR-149 0.086928516 0.014751876 0.10008852 0.065855736 0.064803901 0.122870154
MIR-150 0.035257765 0.044970782 0.013524245 0.01911125 0.040557779 0.009163173
MIR-151 0.001502221 0.00077307 0.010963144 0.004838186 0.006287782 0.006666998
MIR-152 0.034286319 0.010969406 0.017066789 0.003675564 0.013946964 0.000363235
MIR-153-1,2 0.000919004 0.009606747 0.017836767 0.012194694 0.00483984 0.008528321
MIR-154 0.067320132 0.022274012 0.009956041 0.015287597 0.009191066 0.046768417
MIR-155 0.077038308 0.106141428 0.009407303 0.007075424 0.036898819
MIR-181a,c 0.079996174 0.077614756 0.007461971 0.038300523 0.066369259 0.018223267
MIR-181b-1,2 0.008946982 0.011017054 0.002067445 0.007418372 0.010369003
MIR-182 0.100757338 0.055598442 0.07960848 0.237692192 0.073684295 0.058844583
Continued
164
Table A. continued
MIR-183 0.008979481 0.004047088 0.001348589 0.004340321 0.000263908 0.000497427
MIR-184 0.05051926 0.044330218 0.072557946 0.050761004 0.03901425 0.108419895
MIR-185 0.633710937 0.294187381 0.645035255 0.527134645 0.551639041 0.385112116
MIR-186-P 0.935706071 0.446803926 0.303885116 0.462042805 0.469631951 0.309301111
MIR-187 0.007270467 0.000225482 0.001157761 0.018903162 0.000410903 0.000914804
MIR-188b-P 0.000724496 0.00039633 0.000844523 0.001944489 0.000759956 0.004218657
MIR-190 0.00016077 0.000644722 0.000392073
MIR-191 0.000778864 0.000556995 0.000542173 0.014965024
MIR-192 0.006696026 0.002958968 0.011764676 0.024988808 0.012608168 0.005743996
MIR-193 0.01589906 0.009701276 0.002605877 0.000346507 0.012174915 0.003679046
MIR-194-1,2 0.001023204 0.000168363 0.001232811 0.000836408 0.001864715 0.000584266
MIR-195-P 0.20080002 0.091912779 0.176476797 0.274802383 0.19269873 0.318727282
MIR-196a-1 0.00075266 0.00602119 0.025502269 0.005256543 0.004501955 0.008737234
MIR-196a-2 0.020158999 0.016142438 0.005548917 0.018756903 0.004827768 0.004313703
MIR-196b 0.004148833 0.020908049 0.012660226 0.006458653 0.011279579 0.027315363
MIR-197 0.128289662 0.081209976 0.115599288 0.174571254 0.232768779 0.190387929
MIR-198 0.011877079 0.010301322 0.003112217 0.001372084 0.002911026 0.001698491
MIR-199a-1-P 0.490659235 0.057050695 0.735557954 1.05119143 0.879916463 2.030860887
MIR-199a-2-P 0.162976439 0.052733051 0.0281056 0.013863914 0.009744109 0.032226547
MIR-199b-P 0.073831169 0.028130232 0.099536152 0.10838026 0.09472772 0.142055433
MIR-200a-P 2.224986094 0.61231578 3.024940823 2.167233981 3.167045226 1.844070637
MIR-200b 0.00763465 0.007354512 0.072845033 0.058596423 0.021555607 0.059740906
MIR-200c 0.010965055 0.005957201 0.016358887 0.006783925 0.000252496 0.03293596
MIR-203 0.000322681 0.000279717 0.000554647
MIR-204 0.015467586 0.009911398 0.064307638 0.076332641 0.038022204 0.061313812
MIR-205 0.000824276 0.000206456 0.000561284 0.003656465 0.000804026 0.002872373
MIR-206 0.001280679 0.00361721 0.010987957 0.005991716 0.005113015 0.028726488
MIR-208 0.004187299 0.001000292 0.001682885 0.001433102 0.009417615 0.010745176
MIR-210 0.11420006 0.070357609 0.437976516 0.363063529 0.212747999 0.614282172
MIR-211 0.167179727 0.133986659 0.241419969 0.284634791 0.117382844 0.391738932
MIR-212 0.607649507 2.227095262 0.029085722 0.052035947 0.076748479 0.016447481
MIR-213 0.038917917 0.051476482 0.00749061 0.014536648 0.031619798 0.012966522
MIR-214 0.10280425 0.031914686 0.120174351 0.117618542 0.102636798 0.158354846
MIR-215 0.006677625 0.008058862 0.000140962 0.013605591 0.028699979 0.001628544
MIR-216 0.044542113 0.00181394 0.129891274 0.088193089 0.018426367 0.342708984
MIR-217 0.000519976 0.000379363 0.003348429 0.000820813 0.000783614
MIR-218-1,2 0.023693782 0.084363501 0.015582331 0.01008886 0.02302267 0.003839183
MIR-219-1,2 0.002019892 0.016415719 0.007952686 0.008569094 0.008980708 0.00027338
MIR-220 0.002382353 0.004243432 0.003798587 0.005317768 0.000525515 0.001328607
MIR-221 0.045363486 0.206668765 0.00234579 0.010829076 0.048879471 0.001251769
MIR-222-P 0.003004928 0.088710659 0.002082103 0.005599045 0.000902771 0.007808702
MIR-223 0.002536158 0.005514143 0.000849194 0.000363298 0.002199216 0.002855262
MIR-224 0.02191821 0.02538591 0.028971344 0.068689335 0.060831356 0.057696432
MIR-296 0.00089422 0.000193964 0.001173404 0.032340312 0.006783403 0.096706483
MIR-299-P 0.028004798 0.007496133 0.02124658 0.039260692 0.06252084 0.052699932
MIR-301 0.039358194 0.03021299 0.000914754 0.03120901 0.025459837 0.00122564
Continued
165
Table A. continued
MIR-302 0.000438643 0.000743334 0.000550085 0.00239683 0.012472072 0.00166151
MIR-302b 0.009215929 0.006770132 0.01302563 0.01354319 0.017943094 0.023260742
MIR-302c 0.000538611 0.00268758 0.005115213 0.012038796 0.004563745 0.004448112
MIR-302d 0.001196595 0.000598 0.001447207 0.003459445 0.001136237 0.003239474
MIR-320 0.010573108 0.004776736 0.012614514 0.007403522 0.012559214 0.018312549
MIR-321 2.529263454 2.614628126 1.53730766 0.538865609 0.824102944 2.33561409
MIR-323 0.084757733 0.161079943 0.056647532 0.053255688 0.007384009 0.062183512
MIR-324-P 0.027382924 0.020772034 0.032597009 0.029904552 0.042127072 0.030508032
MIR-325 0.023730673 0.008489088 0.018331256 0.004664147 0.006476886 0.010704524
MIR-326-P 0.001163984 0.000345657 0.000807971 0.004226001 0.000790603 0.00441383
MIR-328 0.094916935 0.023315615 0.376005174 0.368961815 0.058289162 0.108346685
MIR-330 0.011098298 0.001304801 0.004215824 0.002236816 0.010305098 0.009432601
MIR-331-P 0.017249189 0.016156329 0.043577309 0.1404588 0.00454517 0.194754412
MIR-335 0.000714167 0.005257783 0.000672328 0.001290817
MIR-337 0.009655227 0.011671912 0.013385751 0.021223155 0.004854431 0.014204156
MIR-338 0.022085623 0.003777932 0.043793587 0.03686966 0.012337565 0.052496768
MIR-339 0.063598622 0.023829686 0.071011349 0.326150426 0.282321806 0.441632858
MIR-340 0.219196827 0.19207162 0.095976926 0.07959692 0.061918742 0.064557227
MIR-342 0.016094274 0.019453031 0.008519163 0.022949578 0.023347518 0.010823815
MIR-345 0.052473209 0.01913323 0.673656907 0.139146056 0.078091264 0.363043523
MIR-346 0.131372418 0.055734377 0.187011633 1.702067697 1.720500092 0.966097981
MIR-361 0.026527432 0.008025018 0.014664096 0.019732772 0.0191621 0.031629782
MIR-367 0.017411313 0.003441667 0.01419365 0.008290518
MIR-368 0.005919593 0.019300075 0.004895244 0.001663394
MIR-369 0.015298408 0.022314592 0.010494316
MIR-370 0.207743295 0.353701752 0.047575427 0.068266775 0.008909545 0.074734641
MIR-371 0.015295969 0.004654935 0.049514965 0.033780428 0.029479012 0.036412155
MIR-372 0.001488946 0.000276836
MIR-373 0.007286965 0.005215133 0.00493249 0.006754358 0.004468675 0.015911779
MIR-374-P 0.001586066 0.00065878 0.000329356 0.000411191
MIR-375 0.018845107 0.012796072 0.079823338 0.076721776 0.010241788 0.054882401
MIR-376 0.684268206 0.96146135 0.103764864 0.093991064 0.065889938 0.132299583
MIR-377 0.061988131 0.037628883 0.007205174 0.006368867 0.001786016 0.01837233
MIR-378 0.001222423 0.000900602 0.002548353 0.002219935 0.003549328 0.003510605
MIR-379 0.215938115 0.423991066 0.029022275 0.028653862 0.005718092 0.017189929
MIR-380 0.019415953 0.097228783 0.003817961 0.017182055 0.025984398
MIR-381 0.001494169 0.000508724 0.000776632
MIR-382 0.001797466 0.017487875 0.002343918 0.005644862 0.000321918 0.001972611
MIR-383 0.00187283 0.000543504 0.003751125 0.003483109 0.00289563 0.005342967
MIR-384 0.000370038 0.00091871
MIR-422b 0.003091468 0.00114528 0.004550325 0.012206081 0.011444339 0.004703813
MIR-423 0.004722377 0.001345595 0.016782389 0.002335283 0.003845286 0.004210295
MIR-424 0.079727421 0.068436096 0.009423116 0.001933843 0.00518589
MIR-425 0.005968921 0.003859785 0.005535466
18S rRNA (CT) 7.79091525 6.9697087 7.26284335 7.50819785 7.5580864 7.96171685
Continued
166
Table A. continued
Tissue Type Adjacent Benign
Pancreatic Tumor
miRNAs 1050005B1(B) OUP2 (T) OUP14 (T) OUP16 (T) OUP20 (T) OUP28 (T)
let-7a-1* 0.001423629 0.000594958 0.000654234
let-7a-2-P 0.070348517 0.41475642 0.054388235 0.153671717 0.273395304 0.069215121
let-7a-3* 0.003195853 0.034800063 0.005204295 0.014361603 0.01200169 0.019794612
let-7b* 0.00973822 0.042247981 0.003221621 0.031400744 0.031384942 0.020265373
let-7c* 0.000310178 0.00149755 0.000284694 0.000326848 0.000723875 0.000183141
let-7d* 0.025884968 0.110129259 0.01759205 0.029142018 0.086463669 0.015816548
let-7e* 0.001361318 0.001234311 0.002509369
let-7f-1* 0.045191064 0.156129409 0.040443185 0.040201233 0.109517959 0.008588948
let-7f-2* 0.006048117 0.003416149 0.002539039 0.003217479
let-7g* 0.025027939 0.066040288 0.016596541 0.032628106 0.058961064 0.016901927
let-7i* 0.017535353 0.066659103 0.020771129 0.039947017 0.081398858 0.076358103
MIR-001-1 0.002811673 0.000108908 0.005656477 0.000213749 0.017297908 0.000274362
MIR-001-2 0.098096184 0.101109623 0.076408498 0.087032501 0.093526718 0.024111665
MIR-007-1 0.008814753 0.021169709 0.023474633 0.019440883 0.013064094 0.027358825
MIR-007-2 0.008383578 0.008165377 9.73217E-05 0.017088038 0.002281971 0.005971564
MIR-007-3 0.133564112 1.379062383 0.259782278 2.40610578 1.68836856 0.098712498
MIR-009-1-P 0.122060755 0.047504211 0.026638662 0.049903111 0.034224989 0.044998464
MIR-009-2-P 0.098047205 0.032441216 0.01455807 0.025395674 0.040686271 0.036625051
MIR-009-3-P 0.000285954 0.000264532 0.000786932 0.00059768
MIR-010a,b 0.000925525 0.073408464 0.012636642 0.017514924 0.032294658 0.031635618
MIR-015a 0.003139291 0.025435173 0.008587057 0.042420475 0.051521356 0.007613352
MIR-015b 0.037206199 0.084688725 0.023812492 0.052495318 0.100748437 0.035067565
MIR-016-1 0.019114984 0.270849479 0.08842511 0.133920068 0.32799219 0.11675353
MIR-016-2 0.139145516 0.025521151 0.020283338 0.095239877 0.067542639 0.059259287
MIR-017-P 0.044288143 0.027135856 0.003290477 0.014054209 0.014257777 0.004727934
MIR-018 0.449438718 0.978574246 0.577999808 0.389764357 1.537229289 0.663102854
MIR-019a-P 0.026767641 0.029440025 0.001385299 0.026498657 0.007963914 0.060079352
MIR-019b-1-P 0.008297876 0.000633804 0.000303771 0.011387103 0.001855173 0.001145669
MIR-019b-2-P 0.000299944
MIR-020 0.000326171 0.000174797 0.000776441 0.000523797 0.000728277 0.000318034
MIR-021 3.32085175 33.03194965 20.00060754 24.40019157 49.81759151 21.54238488
MIR-022-P 0.032683206 0.056601564 0.002437109 0.042205024 0.015249004 0.011769718
MIR-023a 0.017375741 0.170334458 0.093003635 0.092513266 0.061500134 0.117316071
MIR-023b-P 0.02561225 0.200593216 0.009025818 0.084612145 0.042366254 0.056902706
MIR-024-1,2 1.6222974 14.4739142 2.232703103 6.045809702 15.82213487 4.468922635
MIR-025 0.05853855 0.095042969 0.033293213 0.031330619 0.073565893 0.028544064
MIR-026a-1,2 0.004259835 0.028838586 0.0079225 0.007713646 0.010445725 0.023549489
MIR-026b 0.17143889 0.021161867 0.026445186 0.087311958 0.026206434 0.088677602
MIR-027a, b 0.021581865 0.159556105 0.082599589 0.077883205 0.110975943 0.032486668
MIR-028 0.002795153 0.004105197 0.00126089 0.012541694 0.006013315 0.002775389
MIR-029a,c 0.018200108 0.090071327 0.006956831 0.040700027 0.032580722 0.041813817
MIR-029b-1,2
MIR-030a,e 0.020627751 0.01895925 0.004099504 0.022583025 0.006508866 0.034690695
MIR-030b 0.050257625 0.143950801 0.054403581 0.0789416 0.1647504 0.077281777
Continued
167
Table A. continued
MIR-030c-1,2 0.023641384 0.080363584 0.00929344 0.011671583 0.094145691 0.033005415
MIR-030d 0.003100896 0.014624886 0.003271975 0.012337326 0.011237784 0.000267971
MIR-031-P 0.202680377 0.020485085 0.022983048 0.079666145 0.042430879 0.068590571
MIR-032 0.040828003 0.02452186 0.010487797 0.036083837 0.039026445 0.071635999
MIR-033 0.000247156
MIR-034a 0.036099179 0.055252257 0.018020563 0.029261328 0.073340269 0.018140224
MIR-034b 0.001742753 0.000234882 0.001279748 0.000336393 0.000798177 0.000276005
MIR-034c
MIR-092-1 0.000482094 0.001916867 0.00453689
MIR-092-2-P 0.001097092 0.001143311 0.000537272 0.000366962
MIR-093 0.025100185 0.038220639 0.028757148 0.011517262 0.03514242 0.042088053
MIR-095 0.006630502 0.011968127 0.002042306 0.013679393 0.027287752 0.016018741
MIR-096-P 0.82492526 0.054086439 0.052680813 0.067317671 0.053692127 0.053018129
MIR-098 0.864319922 1.989325314 0.710710747 0.726886527 2.311994345 1.287634886
MIR-099a 0.001502566 0.013021101 0.002561091 0.013732504 0.016380473 0.009978862
MIR-099b 0.287747903 0.273889473 0.166356532 0.196485066 0.44922352 0.219059482
MIR-100 0.007726843 1.045013601 0.184805413 0.550541632 0.850119978 0.260610221
MIR-101-1 0.000574251 0.000114148 0.000603556 0.002618999 0.000506199
MIR-101-2 0.053282952 0.114554316 0.052932108 0.074149644 0.091755781 0.054819446
MIR-103-1,2 1.041487713 1.142245073 0.640659155 0.606960724 1.089584714 0.453651731
MIR-105-1,2 0.008822458 0.000253703 0.001499894 0.002588451 0.001859081 0.00510165
MIR-106a-P 0.001913151 0.0010251 0.000257802
MIR-106b 0.154871472 0.377205919 0.331988672 0.127712361 0.403460228 0.130109352
MIR-107 1.246659797 2.014763806 1.058910326 1.266147138 2.079282626 1.014989336
MIR-108 0.0126535 0.000602809 0.006769328 0.004133766 0.00010351 0.000988227
MIR-122a
MIR-124a-1,2,3 0.008766206 0.000679315 0.000866019 0.011991408 0.007437836
MIR-125a 0.02132425 0.103826759 0.032821305 0.090736421 0.131816454 0.080511354
MIR-125b-1 0.00339042 0.436484467 0.048809251 0.362610491 0.333609984 0.086095585
MIR-125b-2 0.006943558 0.028819242 0.000857125 0.030752587 0.00265846 0.009407536
MIR-126 0.073632491 0.406998031 0.062798914 0.450461768 0.472090831 0.17547502
MIR-127 1.491380951 0.369918721 0.344945159 1.547889054 0.322749411 1.650171614
MIR-128a 0.068899357 0.068079215 0.033367651 0.022981505 0.129549008 0.023501148
MIR-128b 0.003559303 0.000823473 0.001592361 0.007884708 0.002342978 0.004101107
MIR-129-2 0.165441299 0.019140857 0.010305594 0.028149685 0.009116937 0.019040459
MIR-130a-P 0.158091677 0.015114051 0.013109486 0.046471969 0.023445248 0.017381827
MIR-130b 0.005957265 0.003137524 0.006230894 0.008432719 0.004700242 0.000470127
MIR-132 0.039175004 0.005173848 0.043977414 0.027373436 0.00887761
MIR-133a-1,2 0.029254763 0.004488091 0.012952603 0.012135241 0.006477363 0.007122044
MIR-133b 0.028256769 0.00455372 0.001958075 0.002333041 0.002116244 0.00247161
MIR-134 0.083809252 0.027093202 0.015784528 0.073104426 0.069236825 0.024672634
MIR-135a-1,2 0.015335477 0.021934912 0.013030714 0.016963652 0.014889136 0.016643487
MIR-135b-P 0.006577668 0.074539927 0.00866846 0.000924352 0.014142308 0.021576618
MIR-136 0.002986287 0.114399228 0.010742327 0.088190225 0.034812322 0.005343529
MIR-137 0.001205778 0.007247988 0.002720449 0.00151674 0.06091669 0.001017882
MIR-138-1,2 0.000732259 0.00065052 0.000227073 0.001188417 0.002637915
Continued
168
Table A. continued
MIR-140 0.000162007 0.000353196 0.000498704
MIR-141 0.008248353 0.018631275 0.024406858 0.008700389 0.012734621 0.022307553
MIR-142-P 0.055352156 0.002938322 0.006623013 0.019342145 0.006219485 0.021121975
MIR-143 0.017013035 0.043010649 0.043236136 0.04795525 0.076879532 0.062566862
MIR-144 0.000170108 0.000327711 0.000218607
MIR-145-P 0.01914608 0.281342658 0.050788509 0.413330454 0.328809838 0.254115022
MIR-146 0.000508526 0.000452192
MIR-147 0.018011839 0.004491567 0.001033817 0.009479696 0.003437581
MIR-148a-P 0.003418715 0.001012706 0.001944396 0.002573135 0.00065437 0.00791107
MIR-148b-P 0.029661088 0.061098498 0.001217379 0.027829997 0.028425605 0.017861903
MIR-149 0.028928239 0.009460841 0.007895842 0.011829407 0.013594815 0.024331137
MIR-150 0.0221614 0.014447636 0.017992356 0.031936844 0.01247748 0.025761628
MIR-151 0.017515164 0.000530026 0.003151417 0.00232387 0.000460497 0.003200594
MIR-152 0.003047563 0.059223363 0.022734395 0.012827916 0.07522262 0.019393084
MIR-153-1,2 0.00310781 0.020732117 0.021598388 0.008297029 0.011625257 0.003217185
MIR-154 0.000790577 0.041700015 0.008057877 0.059609954 0.081907244 0.014092766
MIR-155 0.078521134 0.127762311 0.035422981 0.14405709 0.139463826 0.0821331
MIR-181a,c 0.073427858 0.529327025 0.069205256 0.06624044 0.228176065 0.105751576
MIR-181b-1,2 0.005278691 0.002931564 0.000324859 0.001868653 0.002983088 0.009558584
MIR-182 0.032742625 0.399783329 0.428272195 0.021532626 0.452498956 0.084079685
MIR-183 0.038885544 0.0221167 0.017430882 0.012374033
MIR-184 0.080126197 0.012232056 0.003025155 0.013648456 0.013686699 0.010320249
MIR-185 0.495805204 0.103222202 0.146215881 0.422300122 0.116697282 0.463699016
MIR-186-P 0.382627327 0.52261003 0.119684059 0.303578509 0.220727053 0.377130195
MIR-187 0.002138727 0.000152959 0.00011883
MIR-188b-P 0.000377317 0.000284233 0.000362888 0.000777692 0.000320542
MIR-190
MIR-191 0.000183643
MIR-192 0.010704998 0.000425292 0.000163171 0.00169449
MIR-193 0.001189316 0.007645713 0.000235521 0.005407178 0.02016257 0.00362276
MIR-194-1,2 0.000284146 0.000266272 0.002080237 0.00064955 0.000189386 0.001433465
MIR-195-P 0.231352296 0.024622861 0.02819055 0.067335008 0.029055626 0.073685092
MIR-196a-1 0.007635185 0.00609675 0.031358327 0.007436588 0.006898141 0.01124891
MIR-196a-2 0.0206613 0.007366368 0.003963594 0.035846264 0.004984437 0.008691706
MIR-196b 0.00777359 0.222707758 0.432932622 0.028887248 0.180595502 0.130427507
MIR-197 0.315556625 0.045423706 0.011730025 0.034968096 0.028799845 0.080224103
MIR-198 0.023011643 0.000639717 0.001968135 0.001184933 0.00259598
MIR-199a-1-P 0.744604283 0.014404249 0.092822085 0.320494959 0.00765696 0.217100442
MIR-199a-2-P 0.008845125 0.043923969 0.005639766 0.065001646 0.022967424 0.033399182
MIR-199b-P 0.086970342 0.02069062 0.021915986 0.027048616 0.01322923 0.029196456
MIR-200a-P 4.280903514 0.178786997 0.226108153 0.419195481 0.225827075 0.526664663
MIR-200b 0.02990711 0.053776707 0.070716879 0.011251862 0.042175833 0.007332864
MIR-200c 0.001475001 0.000135777 0.000144547 0.004377857
MIR-203 0.00034598 0.004857104 0.004901797 0.000404506
MIR-204 0.053025195 0.002795896 0.002823991 0.009749366 0.00473756 0.003819462
MIR-205 0.000773773 0.000606239 0.012370112 0.00234449
Continued
169
Table A. continued
MIR-206 0.001984982 0.015136997 0.002018393
MIR-208 0.002603413 0.001226127 0.009615127 0.010792376
MIR-210 0.166658906 0.113925864 0.419966577 0.058884534 0.127703801 0.05291181
MIR-211 0.13028716 0.029213565 0.021780259 0.171086588 0.05940894 0.085007537
MIR-212 0.024943873 0.963572563 0.106369277 0.686635365 0.538302626 0.105167152
MIR-213 0.011435187 0.200780591 0.014729895 0.062404054 0.125496442 0.019430961
MIR-214 0.119638764 0.009174687 0.017236915 0.070371475 0.0179198 0.057123951
MIR-215 0.04486903 0.004663094 0.009870688 0.006479021 0.004399442 0.013177893
MIR-216 0.015583988 0.004792052 0.0011552 0.002476852 0.000632454 0.016206581
MIR-217 0.000193888 0.000336781
MIR-218-1,2 0.008193751 0.300640801 0.037268948 0.103573804 0.104541949 0.117272209
MIR-219-1,2 0.002966792 0.00259582 0.003897984 0.001079005 0.009428942
MIR-220 0.001172217 0.000132585 0.000299487 0.001936894 0.004598896 0.005858608
MIR-221 0.031372098 0.073490329 0.034905034 0.060384562 0.087033545 0.067769788
MIR-222-P 0.000281751 0.020685444 0.001332613 0.011253941 0.035167751 0.011913586
MIR-223 0.000359368 0.001212042 0.000286561 0.000341708 0.003150087 0.001435801
MIR-224 0.058444166 0.074824016 0.026717159 0.013940847 0.070294707 0.043150769
MIR-296 0.009403023 0.002735499 0.001492529 0.000268309 0.015296086 0.000389812
MIR-299-P 0.042670322 0.002600696 0.001867947 0.000718676 0.000187316 0.008094348
MIR-301 0.022523664 0.087728549 0.01676406 0.030743369 0.102489099 0.061407453
MIR-302 0.009143792 0.016078573 0.011714636 0.012898931 0.00770312
MIR-302b 0.016169275 0.001778454 0.00455068 0.010732296 0.003238623 0.01810689
MIR-302c 0.01321861 0.001064876 0.001301286 0.000354531 0.004456342
MIR-302d 0.000685746 0.000747311 0.000296246 0.001801801
MIR-320 0.010840314 0.006055577 0.004623993 0.00506651 0.003109003 0.005894559
MIR-321 0.88907148 1.522499463 0.855360754 6.581710496 3.270910693 3.390836141
MIR-323 0.017313423 0.035881779 0.002077474 0.119722986 0.090518116 0.010950485
MIR-324-P 0.02337608 0.010188664 0.017434683 0.011538789 0.0023143 0.007463596
MIR-325 0.006190612 0.013532055 0.004005924 0.008328163 0.015322955 0.005070055
MIR-326-P 0.00186242 0.001027406 0.000284791 0.000108019 0.000235086
MIR-328 0.076486732 0.009429802 0.004137039 0.006837943 0.012402273 0.027921612
MIR-330 0.005196489 0.014491364 0.001816003 0.001070896 0.009896941 0.002220484
MIR-331-P 0.007967384 0.028540427 0.00186289 0.00131094 0.006459387 0.003468454
MIR-335 0.00111324 0.000267524
MIR-337 0.007868977 0.022216744 0.000640083 0.006128425 0.004491249
MIR-338 0.013068885 0.000175346 0.001089081 0.0066515 0.005335516
MIR-339 0.344605903 0.086379048 0.04340805 0.048272658 0.11314752 0.077121535
MIR-340 0.072734433 0.247468057 0.092358812 0.209861593 0.384719102 0.087591007
MIR-342 0.018975344 0.008843126 0.011040363 0.003878336 0.011063149 0.003529608
MIR-345 0.063969425 0.003089068 0.011244824 0.006105734 0.009219951 0.014441967
MIR-346 0.813830254 0.031585106 0.016382041 0.077717029 0.023651449 0.314494691
MIR-361 0.022147886 0.005055518 0.003786216 0.016090903 0.004675649 0.017377039
MIR-367 0.007063752 0.008037384 0.003605343 0.007813283 0.002367325 0.003034458
MIR-368 0.010289472 0.004241377 0.01557411 0.001370014 0.01612779
MIR-369 0.005407072 0.021170447 0.006108604
MIR-370 0.020032424 0.274228842 0.066135006 0.390578535 0.33460731 0.073458364
Continued
170
Table A. continued
MIR-371 0.039729524 0.000673832 0.005368888 0.040970607 0.001813638 0.02209352
MIR-372
MIR-373 0.007505463 0.000496482 0.001702052 0.001279374 0.016912941 0.001103472
MIR-374-P 0.003430427 0.001290493 0.000720987 0.001141035 0.001289678
MIR-375 0.015131059 0.02463161 0.034556344 0.02256787 0.014556662 0.016307829
MIR-376 0.016315537 0.661370554 0.074945877 0.880770815 1.343314274 0.239373038
MIR-377 0.007414514 0.008789405 0.000569931 0.015128446 0.010797659 0.005941476
MIR-378 0.000352964 0.002082651 0.000334934 0.000669631 0.00084637
MIR-379 0.016226828 0.268418445 0.036440029 0.456266162 0.641024263 0.085209292
MIR-380 0.0117664 0.054731576 0.008789674 0.136483132 0.186616295 0.014466744
MIR-381
MIR-382 0.002117621 0.004880133 0.023802816 0.000973482 0.000921405
MIR-383 0.002635481 0.000890139 0.000407181 0.000871883 0.0008852 0.001569394
MIR-384 0.000204873
MIR-422b 0.014706965 0.000402593 0.001140709 0.006732409 0.001049863 0.000696487
MIR-423 0.005866872 0.000818558 0.000836558 0.002009754 0.001158201 0.002887534
MIR-424 0.002357025 0.089123872 0.008895522 0.087598475 0.052309322 0.022706963
MIR-425 0.005784848 0.004630251 0.002615099 0.007988618 0.006540982 0.005011067
18S rRNA (CT) 7.43896825 6.87257765 6.64977285 7.778018 6.7326428 7.58807
Tissue Type Pancreatic Tumor
miRNAs OUP48 (T) OUP91 (T) OUP97 (T) OUP31 (T) OUP33 (T) OUP36 (T)
let-7a-1* 0.002375689 0.000251654
let-7a-2-P 0.174871619 0.062582913 0.140469831 0.251181144 0.397074417 0.072780018
let-7a-3* 0.002451198 0.009024262 0.009691037 0.021109403
let-7b* 0.017043537 0.007846468 0.018585402 0.046381188 0.026275596 0.02146025
let-7c* 0.000618048 0.000456827 0.000594125 0.002815045
let-7d* 0.020703838 0.037607263 0.027132264 0.135711565 0.155384911 0.03714613
let-7e* 0.002047996 0.000771036 0.00153489 0.002532193 0.001527224
let-7f-1* 0.053401518 0.065145445 0.059576068 0.179328609 0.286323367 0.063951266
let-7f-2* 0.004326124 0.003916182 0.005949953 0.014587938 0.001628424
let-7g* 0.011708265 0.018601015 0.011599932 0.075882266 0.096861075 0.011340886
let-7i* 0.019014938 0.070566468 0.072104855 0.189752108 0.023663811
MIR-001-1 0.000454842 0.014223544 0.001969135
MIR-001-2 0.043985126 0.018191416 0.086214792 0.068748096 0.090368095 0.040501488
MIR-007-1 0.017389926 0.0053618 0.012899118 0.023399241 0.069480253 0.026008134
MIR-007-2 0.004963586 0.004264282 0.005312526
MIR-007-3 0.837572842 0.010016457 0.126406427 0.336669532 0.219981837 0.422214116
MIR-009-1-P 0.053929354 0.032333783 0.054032405 0.078948659 0.080194845 0.061331146
MIR-009-2-P 0.048408898 0.007968678 0.011184567 0.036210178 0.134006819 0.103766396
MIR-009-3-P 0.000326805 0.000142562 0.000946667
MIR-010a,b 0.003965822 0.006778899 0.031493474 0.05793922 0.027032548 0.00717817
MIR-015a 0.013663601 0.0095409 0.018514376 0.041288693 0.035979572 0.02058099
MIR-015b 0.031978056 0.020280234 0.048217636 0.087076943 0.057527609 0.017516583
MIR-016-1 0.050341031 0.076212007 0.151176858 0.245329235 0.414347413 0.077597782
MIR-016-2 0.039068768 0.023005293 0.050641299 0.049920911 0.061398632 0.079422122
Continued
171
Table A. continued
MIR-017-P 0.004092378 0.023879809 0.002761863 0.040519272 0.018091895 0.032053845
MIR-018 0.404686203 0.578040555 0.32674311 0.598454334 0.968630489 0.595679914
MIR-019a-P 0.039188538 0.037596238 0.003551665 0.036859864 0.032937145 0.10991565
MIR-019b-1-P 0.008293027 0.001173528 0.005369914 0.00386905 0.003356515 0.01240839
MIR-019b-2-P 0.000520497
MIR-020 0.007016487 0.000269524 0.002685945 0.003106072
MIR-021 8.915153074 9.29252471 20.14473217 33.46846708 60.29188037 6.203911986
MIR-022-P 0.033302564 0.025851963 0.00192182 0.050022417 0.011647941 0.017909529
MIR-023a 0.082847448 0.041920177 0.096394305 0.170793748 0.168910079 0.043063674
MIR-023b-P 0.07670506 0.019845003 0.032034121 0.156090709 0.050648236 0.150752749
MIR-024-1,2 4.696952713 2.766813919 7.782349215 12.63394305 11.6705797 5.325519328
MIR-025 0.008971367 0.022043867 0.02162083 0.055577377 0.098841747 0.025888204
MIR-026a-1,2 0.003637923 0.001550269 0.015720541 0.029696435 0.03440193 0.01031878
MIR-026b 0.041885322 0.02917748 0.055386885 0.063960036 0.097817427 0.078311458
MIR-027a, b 0.049458852 0.053785743 0.068466198 0.209648741 0.21286399 0.062765353
MIR-028 0.002300939 0.000167934 0.002243287 0.003752732 0.005140997 0.003519445
MIR-029a,c 0.076721199 0.06576351 0.021734334 0.094951465 0.116818391 0.03655622
MIR-029b-1,2
MIR-030a,e 0.01117558 0.006586796 0.013026124 0.035515007 0.01795595 0.018998169
MIR-030b 0.039549755 0.025803912 0.078978798 0.236727968 0.081111671 0.133680063
MIR-030c-1,2 0.01622249 0.028214794 0.008191738 0.059599357 0.081563038 0.042306869
MIR-030d 0.007653008 0.004551354 0.008924195 0.049774584 0.015872132
MIR-031-P 0.029271907 0.017409935 0.041140274 0.090789173 0.101459884 0.108782751
MIR-032 0.02714509 0.023998011 0.025903758 0.043004593 0.048055315 0.077554845
MIR-033 0.003852279 0.000331804
MIR-034a 0.012109395 0.028234857 0.041777608 0.107283422 0.085745889 0.021213695
MIR-034b 0.000462068 0.001007995 0.000855576 0.000907242 0.001068435
MIR-034c
MIR-092-1 0.00534726 0.00444817
MIR-092-2-P 0.001600195 0.002005769
MIR-093 0.001848427 0.001536199 0.028375964 0.028475097 0.077782549 0.024975722
MIR-095 0.019226108 0.003740904 0.004621919 0.003513477 0.011507305 0.000876822
MIR-096-P 0.0581947 0.023237564 0.065071279 0.106536934 0.095742303 0.226503181
MIR-098 1.136349063 0.654748892 1.268561725 2.324841835 3.424253049 1.019385487
MIR-099a 0.009191083 0.003225301 0.002005711 0.012794181 0.007040794 0.005683736
MIR-099b 0.164961478 0.085089511 0.137374413 0.271714426 0.252083102 0.16137866
MIR-100 0.308345152 0.273155357 0.431133693 0.452482866 1.021568057 0.103461271
MIR-101-1 0.000199618 0.000622726 0.000419343 0.001298185 0.000910355
MIR-101-2 0.032801124 0.04497843 0.048809566 0.104415556 0.212174961 0.225216935
MIR-103-1,2 0.275715555 0.263741156 0.611026019 0.976355921 1.293323174 0.372128997
MIR-105-1,2 0.00590601 0.001850064 0.004099411 0.004145807 0.008903716 0.010205921
MIR-106a-P 0.003122135
MIR-106b 0.049369781 0.064434208 0.191940321 0.324217433 0.574997393 0.110564046
MIR-107 0.480582516 0.612008298 1.169745533 1.792514848 3.124857322 0.612717609
MIR-108 0.005455694 0.001608269 0.00298808 0.0040745 0.002244292 0.0035056
MIR-122a 0.000344241 0.000526904
Continued
172
Table A. continued
MIR-124a-1,2,3 0.001364448 0.004952929 0.003923339 0.002345384 0.001712259
MIR-125a 0.017368471 0.025818663 0.055238491 0.096971833 0.093411943 0.077564172
MIR-125b-1 0.317002049 0.24086449 0.174914491 0.181022015 0.272093768 0.171111689
MIR-125b-2 0.00808599 0.001639445 0.012867305 0.025264261 0.008707521
MIR-126 0.115181472 0.252049696 0.24751 0.143317389 0.682134114 0.135155087
MIR-127 1.33315966 0.754465648 0.908663932 0.53615055 0.7704164 0.838639518
MIR-128a 0.013267015 0.030986103 0.034711132 0.080185605 0.114472528 0.045839837
MIR-128b 0.003905234 0.00192626 0.00411876 0.00227602 0.002182065 0.005930637
MIR-129-2 0.026788908 0.002982398 0.015706094 0.023302605 0.026883025 0.098735672
MIR-130a-P 0.024839614 0.011434365 0.013705737 0.028605107 0.038794658 0.067236839
MIR-130b 0.002289573 0.004774959 0.012460362 0.000379298
MIR-132 0.019150244 0.022916613 0.007834352 0.009723336 0.015585846 0.013192472
MIR-133a-1,2 0.001381522 0.033408734 0.004321917 0.007171284 0.019136792 0.004577611
MIR-133b 0.006419293 0.002147493 0.001268783 0.003154369 0.014693077 0.002080704
MIR-134 0.032487692 0.031911 0.037249767 0.046176322 0.051722177 0.03875105
MIR-135a-1,2 0.026150876 0.003103367 0.01236813 0.031493918 0.017255437 0.045204955
MIR-135b-P 0.008201802 0.002568828 0.023474548 0.121218649 0.015258514 0.006694241
MIR-136 0.056375439 0.030632685 0.040394249 0.027810733 0.037016 0.029534075
MIR-137 0.001365421 0.000463725 0.003589698 0.003332088 0.00189424 0.001392531
MIR-138-1,2 0.00116473 0.000227122 0.000793388 0.000197738
MIR-139 0.003172905 0.004768797 0.00459128 0.005706983 0.003475921 0.017453519
MIR-140
MIR-141 0.006231754 0.003402831 0.009246232 0.005372581 0.024837001 0.03296751
MIR-142-P 0.012956219 0.022137884 0.011626311 0.01949134 0.029117793 0.039921167
MIR-143 0.016016332 0.019204053 0.032429256 0.050116677 0.104210772 0.012483902
MIR-144 0.017091676 0.000239447
MIR-145-P 0.161045982 0.050396701 0.069283457 0.570052647 0.395361781 0.053981569
MIR-146 0.000688781 0.003327388 0.002044179 0.00084399
MIR-147 0.001826336 0.009561288 0.009450898 0.008325547 0.001576685
MIR-148a-P 0.003982496 0.002576764 0.001939047 0.002951064 0.003350569 0.00358937
MIR-148b-P 0.031666255 0.009158904 0.015597809 0.174367983 0.05535254 0.046611047
MIR-149 0.020191621 0.007242094 0.026685756 0.019770266 0.022811305 0.035755592
MIR-150 0.020697618 0.038570593 0.02834126 0.014354223 0.121849042 0.034709941
MIR-151 0.00255306 0.001736121 0.004902219 0.001588551 0.002996407 0.003931311
MIR-152 0.016226533 0.005885655 0.020613999 0.067066474 0.073049075 0.007475961
MIR-153-1,2 0.002979602 0.002101668 0.019633997 0.02159845 0.016826102 0.023919478
MIR-154 0.013885752 0.031618344 0.029901149 0.061843812 0.061107841 0.013197548
MIR-155 0.050208679 0.097400138 0.08282248 0.1228496 0.4502786 0.017813548
MIR-181a,c 0.042972501 0.040023485 0.229723558 0.270278483 0.384125546 0.060013597
MIR-181b-1,2 0.001364315 0.001786362 0.002429174 0.005062249 0.013692473 0.00431506
MIR-182 0.04543921 0.047703308 0.206746558 0.505949595 0.658809982 0.12650109
MIR-183 0.006139635 0.001891321 0.005095243 0.011320548 0.016274729 0.008687253
MIR-184 0.014254553 0.001125258 0.00617167 0.009820942 0.018403753 0.022041561
MIR-185 0.330303708 0.181212342 0.317220568 0.248436331 0.381816358 0.333350829
MIR-186-P 0.34728658 0.12979868 0.229974008 0.703396991 0.509581681 0.563277293
MIR-187 0.006272037 0.000563867
Continued
173
Table A. continued
MIR-188b-P 0.000487728 0.000127221 0.000206177 0.000814204
MIR-190
MIR-191 0.000134575 0.000717139
MIR-192 0.000892925 0.00131462 0.006106586 0.00463612 0.001627811 0.001621101
MIR-193 0.003313716 0.006684434 0.010013076 0.034054879 0.012060008
MIR-194-1,2 0.000181649 0.000299554 0.000191336 0.000381267 0.000162547
MIR-195-P 0.056985654 0.022465136 0.085626889 0.090847402 0.099323597 0.142903655
MIR-196a-1 0.003030552 0.00140209 0.014818961 0.004267677 0.002479721
MIR-196a-2 0.013513204 0.005559592 0.015160622 0.026773732 0.032253551 0.016411976
MIR-196b 0.009665909 0.022325157 0.587951566 0.108872015 0.090967109 0.013453186
MIR-197 0.019066073 0.024411904 0.042409366 0.070692064 0.130103738 0.033690595
MIR-198 0.00805707 0.001519203 0.003746865 0.013813398 0.002623591 0.003136449
MIR-199a-1-P 0.124099488 0.041369279 0.085209448 0.166098399 0.062444522 0.26750286
MIR-199a-2-P 0.066122362 0.015239201 0.009595331 0.2501125 0.075400052 0.020576134
MIR-199b-P 0.017801431 0.011948962 0.030016368 0.037361821 0.059447701 0.04485875
MIR-200a-P 0.391462514 0.154941457 0.267121632 0.708893383 0.680456018 1.944302533
MIR-200b 0.003986881 0.034661015 0.069868722 0.003002542 0.028430075
MIR-200c 0.000170232 0.000847621 0.000436411 0.001417595 0.004711308
MIR-203 0.008457119 0.011767159
MIR-204 0.00699417 0.004203253 0.012102956 0.004516252 0.005412134 0.012372014
MIR-205 0.000981725 0.004389103 0.018311878 0.000417331 0.001011072
MIR-206 0.000113684 0.002421001 0.000191598 0.01517024
MIR-208 0.000189051 0.007420385 0.005829808 0.001003326 0.001656682
MIR-210 0.012718877 0.014630462 0.088438368 0.180715677 0.11866154 0.105044987
MIR-211 0.045200015 0.040064662 0.0292698 0.075433782 0.130469349 0.167076904
MIR-212 1.207396538 0.730775473 0.116234175 0.072442772 0.189771641 0.297681448
MIR-213 0.001272518 0.035887597 0.05703806 0.123255426 0.189045684 0.030800161
MIR-214 0.056898624 0.018039137 0.052388491 0.025057731 0.038034431 0.050644616
MIR-215 0.007164139 0.004898121 0.012054409 0.017332644 0.024663192 0.009860259
MIR-216 0.001988267 0.000144878 0.004115875 0.002819579 0.002017466 0.121120017
MIR-217 0.000178382
MIR-218-1,2 0.050288073 0.022986556 0.079675127 0.06967694 0.131260371 0.024784814
MIR-219-1,2 0.004825463 0.008775561 0.035731563 0.000222407
MIR-220 0.002426513 0.001861135 0.000215074 0.007354689 0.016709679 0.001842324
MIR-221 0.061594851 0.051107475 0.073181953 0.087411692 0.077621295 0.028454344
MIR-222-P 0.052140711 0.014320926 0.005309734 0.061849042 0.028799195 0.017971003
MIR-223 0.000614763 0.000588934 0.000730995 0.014426817 0.001354051
MIR-224 0.022395747 0.003697312 0.005293577 0.041010342 0.031611226 0.015974789
MIR-296 0.006148843 0.003639805 0.013397567 0.003877334
MIR-299-P 0.009718572 0.005090384 0.004656523 0.003254094 0.00444764 0.017297888
MIR-301 0.02935791 0.016784154 0.047597141 0.096108882 0.149210123 0.030944584
MIR-302 0.004501982 0.01065104 0.022258888 0.005659859
MIR-302b 0.009351176 0.00651674 0.003848187 0.00264148 0.007051258 0.006084375
MIR-302c 0.002000653 0.003851216 0.00848551 0.002829871 0.001354076
MIR-302d 0.000383137 0.000840641 0.001316292 0.00099471
MIR-320 0.004912525 0.003313739 0.002829148 0.009143895 0.007145246 0.007845435
Continued
174
Table A. continued
MIR-321 0.730240237 2.143619907 1.072759531 2.758076684 4.626782623 2.438901037
MIR-323 0.035229494 0.022633839 0.01295367 0.051967774 0.03981533 0.108028675
MIR-324-P 0.013873081 0.007904474 0.005453166 0.011117676 0.027093072 0.022761607
MIR-325 0.003588706 0.003889537 0.007835085 0.044388202 0.002457333 0.001112918
MIR-326-P 0.000178388 0.000413953 0.001183104 0.002655551
MIR-328 0.014531401 0.005604581 0.009592082 0.014765859 0.02834414 0.337895424
MIR-330 0.001622196 0.004279635 0.004116745 0.012288708 0.008955501 0.005966658
MIR-331-P 0.008185156 0.002884108 0.007223695 0.031697735 0.007292493 0.033477271
MIR-335 0.000306528
MIR-337 0.024922776 0.005490347 0.009495105 0.006115932 0.003321689 0.011396757
MIR-338 0.000349397 0.000837554 0.010565389 0.001161091 0.001489948 0.010969767
MIR-339 0.026842002 0.027951889 0.053964285 0.075437939 0.134201418 0.099021301
MIR-340 0.091694249 0.09829335 0.163884943 0.245991278 0.571337377 0.165409582
MIR-342 0.000867772 0.015386204 0.019056714 0.005767061 0.04089486 0.010342177
MIR-345 0.013952114 0.007279442 0.00651134 0.007362307 0.024036303 0.02807346
MIR-346 0.280606546 0.096020957 0.095327067 0.135547092 0.06619804 0.083407604
MIR-361 0.008079104 0.006357958 0.008870165 0.010324299 0.009656987 0.012053444
MIR-367 0.007694309 0.007232177 0.007174038 0.006465353 0.007622581
MIR-368 0.003183887 0.001860413 0.001435985 0.003199552 0.002825024 0.003548283
MIR-369 0.007039214 0.010129186
MIR-370 0.184238831 0.088917557 0.135775137 0.227052696 0.21696772 0.105219953
MIR-371 0.011481883 0.001842655 0.004946263 0.015855543 0.006569106 0.014918298
MIR-372 0.000487635
MIR-373 0.0040842 0.001066271 0.000473402 0.006274535 0.004308892
MIR-374-P 0.001859374 0.001324964 0.00252264
MIR-375 0.008452097 0.007444286 0.012015069 0.006645793 0.006249317 0.1405951
MIR-376 0.307425144 0.450474273 0.403234706 0.616346724 0.539294348 0.251553247
MIR-377 0.001930621 0.003229018 0.011552058 0.003000158 0.004808737 0.00586711
MIR-378 0.001594542 0.000316037 0.001197333
MIR-379 0.100746426 0.110979616 0.187693842 0.206098094 0.195760734 0.091600274
MIR-380 0.046818307 0.004368401 0.037231504 0.037260299 0.018951572 0.006962097
MIR-381 0.001043082 0.00095589 0.002400681
MIR-382 0.003015705 0.0013447 0.003908465 0.002913046 0.000666651 0.006236149
MIR-383 0.000843659 0.000465562 0.000684608 0.001304542 0.002071034 0.002102763
MIR-384 0.000517599
MIR-422b 0.003082095 0.000902503 0.00202931 0.001059912 0.001412219 0.006441202
MIR-423 0.002383671 0.000375396 0.00074112 0.00205373 0.000865719 0.004851127
MIR-424 0.010583852 0.033383791 0.025013395 0.083420228 0.043011949 0.03351759
MIR-425 0.004346291 0.003831344 0.00959848 0.005350899 0.006846412 0.004123022
18S rRNA (CT) 7.44394 6.93464755 7.72409115 7.0264585 7.58747125 7.34983915
Tissue Type Pancreatic Tumor
miRNAs OUP42 (T) OUP105 (T) OUP122 (T) OUP123 (T) OUP135 (T) OUP139 (T)
let-7a-1* 0.011153874
let-7a-2-P 0.688322831 0.248999322 0.236854308 0.066422711 0.080029484 0.402757796
let-7a-3* 0.012142239 0.003610824 0.004198746 0.013842985 0.019092422
Continued
175
Table A. continued
let-7b* 0.014714647 0.017323169 0.016879861 0.001820256 0.018691063 0.045821577
let-7c* 0.000195859 0.00015392 0.000376859 0.002790285
let-7d* 0.100287331 0.068949563 0.047733811 0.037614622 0.048940611 0.077217487
let-7e* 0.000404457 0.00304275 0.000922099 0.001784704
let-7f-1* 0.106961927 0.120782448 0.036984275 0.018973734 0.063442367 0.160077719
let-7f-2* 0.003271698 0.001138562 0.001407034 0.000841361 0.001834153
let-7g* 0.090000528 0.03702743 0.013058602 0.001136373 0.009316543 0.044855149
let-7i* 0.097010705 0.128698908 0.109011884 0.035167606
MIR-001-1 0.002127454 0.000597795 0.005301019 0.000194888
MIR-001-2 0.058387699 0.107542977 0.136596964 0.029222095 0.050836497 0.059622737
MIR-007-1 0.031256992 0.036693205 0.063844419 0.032762277 0.024094237 0.044438623
MIR-007-2 0.000388743 0.00211759 0.006107399 0.003893446
MIR-007-3 0.402919007 0.012855974 0.44454067 0.039732239 0.011105552 0.227010498
MIR-009-1-P 0.041234287 0.047822114 0.134174723 0.041980711 0.140554634 0.10498524
MIR-009-2-P 0.050717318 0.072867976 0.138929639 0.040229341 0.111340272 0.099447294
MIR-009-3-P 0.000161224 0.000537159 0.003824657
MIR-010a,b 0.015558068 0.02495436 0.019885433 0.026049995 0.032965458 0.070620553
MIR-015a 0.061154407 0.045896429 0.008106494 0.026616652 0.006679831 0.048207366
MIR-015b 0.044129945 0.065482046 0.050257469 0.041261524 0.035976931 0.062363833
MIR-016-1 0.408619446 0.122116768 0.162899583 0.093360035 0.109966875 0.307499276
MIR-016-2 0.038267919 0.054911732 0.139097589 0.061869403 0.114243574 0.052586304
MIR-017-P 0.081200773 0.025263034 0.028738863 0.005222739 0.017970513 0.012490988
MIR-018 2.298142519 0.676080162 0.489542432 0.195482236 0.279761224 0.74350684
MIR-019a-P 0.037793941 0.048105619 0.0524743 0.019596943 0.027302728 0.024943469
MIR-019b-1-P 0.00600025 0.001176736 0.012676799 0.012386384 0.00093186
MIR-019b-2-P 0.001397596
MIR-020 0.000174162 0.000904387 0.0009279 0.000507017 0.000134285
MIR-021 21.08385499 24.11291594 27.11143966 15.06278798 19.98249633 35.43957553
MIR-022-P 0.043107696 0.04812201 0.030614795 0.019061988 0.016213894 0.023713352
MIR-023a 0.131323341 0.102545829 0.162772775 0.044849817 0.086546688 0.265839782
MIR-023b-P 0.023038958 0.069965995 0.101711931 0.022495245 0.045016429 0.165166433
MIR-024-1,2 10.83605915 14.83697133 8.224633086 3.472530629 5.262918009 11.16399067
MIR-025 0.074282582 0.043745433 0.05960575 0.024737893 0.034130087 0.058610742
MIR-026a-1,2 0.017757543 0.019216597 0.008900541 0.004484721 0.01129557 0.031360881
MIR-026b 0.02275712 0.046647246 0.096765013 0.060787244 0.080537803 0.06452855
MIR-027a, b 0.119314747 0.139287454 0.13460961 0.075082028 0.09326124 0.157295077
MIR-028 0.00247522 0.008746916 0.010342386 0.010647182 0.004371646 0.003794492
MIR-029a,c 0.179349455 0.074022066 0.039110216 0.033399526 0.031124024 0.061580544
MIR-029b-1,2 0.000499387 0.003487473
MIR-030a,e 0.014041553 0.024515478 0.037383924 0.021832058 0.019133557 0.009745699
MIR-030b 0.169153012 0.085862573 0.068731575 0.098531335 0.040527105 0.079761366
MIR-030c-1,2 0.104675359 0.035320087 0.023997024 0.025358398 0.014498037 0.007513454
MIR-030d 0.035200525 0.011324834 0.015128993 0.012044129 0.005849646
MIR-031-P 0.05121525 0.065614784 0.223803161 0.061077419 0.187569209 0.115240954
MIR-032 0.049790654 0.034388267 0.035498203 0.002220442 0.029697163 0.06127016
MIR-033 0.000627595 0.002387511
Continued
176
Table A. continued
MIR-034a 0.029164967 0.043462523 0.02889291 0.036105838 0.020694281 0.053524414
MIR-034b 0.000780442 0.001631532 0.000859591 0.001109632 0.000522144
MIR-034c
MIR-092-1 0.006665484 0.003569446 0.006805377 0.011679865 0.006086105 0.000951352
MIR-092-2-P 0.000863784 0.003697936 0.001322151
MIR-093 0.051227091 0.02832441 0.06847102 0.014210119 0.024299677 0.039661139
MIR-095 0.003585117 0.008257219 0.011091958 0.008648759 0.00662366
MIR-096-P 0.040894929 0.071872801 0.316341628 0.059266942 0.148826869 0.104301508
MIR-098 3.556540936 2.039094616 1.688527668 0.816042061 1.053010571 2.129961597
MIR-099a 0.015863454 0.012654477 0.017117607 0.007352777 0.002565888 0.018004314
MIR-099b 0.40525005 0.270088893 0.349223323 0.146795985 0.096843727 0.326844181
MIR-100 1.402372168 0.458825894 0.508039586 0.167851626 0.263521197 1.040547929
MIR-101-1 0.000479077 0.001160504 0.000243918 0.022788597 0.003442366
MIR-101-2 0.138866477 0.106064827 0.072316458 0.065702379 0.082473156 0.126623178
MIR-103-1,2 0.839665298 0.659709013 0.576691362 0.331092772 0.460798817 1.137816904
MIR-105-1,2 0.001121142 0.00480088 0.002384874 0.003385044 0.013864274 0.007154315
MIR-106a-P 0.002705627 0.000270455
MIR-106b 0.504079528 0.235635812 0.193303856 0.084574434 0.145761581 0.317673559
MIR-107 1.99626157 1.435887499 1.24187229 0.722537524 0.948884845 2.270244089
MIR-108 0.003581982 0.001794761 0.006841106 0.000669585 0.005569042 0.001514441
MIR-122a 0.000358526
MIR-124a-1,2,3 0.004069764 0.000596661 0.002231204 0.00698847 0.000692076
MIR-125a 0.137858466 0.094014117 0.102931918 0.04589696 0.030027245 0.088934495
MIR-125b-1 0.687723128 0.538242314 0.202496702 0.044163886 0.051385724 0.303207095
MIR-125b-2 0.005064144 0.00106304 0.000804029 0.000535104 0.011317369
MIR-126 0.775452205 0.259144238 0.284959999 0.17001046 0.079755174 0.408178591
MIR-127 0.254498184 0.487857164 1.517319411 1.593874369 2.385665741 1.753283652
MIR-128a 0.079265109 0.065503428 0.02570827 0.011110383 0.028262461 0.104063311
MIR-128b 0.000213193 0.003483577 0.003364488 0.003775118 0.006075157 0.002646146
MIR-129-2 0.01462178 0.022534749 0.133509316 0.015615845 0.098493131 0.030059158
MIR-130a-P 0.017024365 0.024179869 0.112037905 0.031212061 0.117510243 0.038951574
MIR-130b 0.002902601 0.00128909 0.021757443 0.002630986 0.001409979
MIR-132 0.071470405 0.034558507 0.006556369 0.012296222 0.00041139 0.005514002
MIR-133a-1,2 0.002673943 0.017492505 0.017410794 0.013269887 0.036008752 0.009496232
MIR-133b 0.002362342 0.010749883 0.019756995 0.010868235 0.004935281 0.006026021
MIR-134 0.047638053 0.071040632 0.130854035 0.035424527 0.096600899 0.034224444
MIR-135a-1,2 0.014094638 0.009156892 0.024439309 0.011381902 0.011376763
MIR-135b-P 0.05486412 0.007979097 0.01601553 0.064516654 0.01762261 0.102299743
MIR-136 0.782894476 0.017599134 0.05423084 0.025889034 0.013439189 0.034199699
MIR-137 0.001143579 0.027448894 0.000974664 0.001004018 0.003740879
MIR-138-1,2 0.000116578 0.00024647 0.00020945 0.000448854
MIR-139 0.002300581 0.004433939 0.025558223 0.006822657 0.017848794 0.006249835
MIR-140
MIR-141 0.003181201 0.013808584 0.006154301 0.012339677 0.021613266 0.019160877
MIR-142-P 0.020230247 0.023098408 0.059933931 0.015194425 0.049406406 0.037645466
MIR-143 0.026303667 0.086199554 0.067875081 0.017577381 0.06803052 0.131543642
Continued
177
Table A. continued
MIR-144 0.006141817 0.000140749
MIR-145-P 0.021075005 0.867642254 0.40671788 0.087233671 0.146327306 0.99943796
MIR-146 0.001371395 0.000216433 0.001161194
MIR-147 0.001875832 0.000177472 0.012228407 0.014023822 0.002903713 0.005259396
MIR-148a-P 0.000490003 0.002118387 0.006395987 0.009903625 0.008355729 0.006930076
MIR-148b-P 0.034533019 0.055854612 0.057463386 0.034314649 0.018214673 0.039426458
MIR-149 0.01285908 0.014443005 0.086374114 0.017665182 0.069421696 0.025285111
MIR-150 0.104112325 0.029983398 0.036489111 0.003204711 0.022706583 0.056800617
MIR-151 0.005297955 0.001235936 0.007762574 0.006522508 0.010315903 0.00304762
MIR-152 0.021103546 0.032029681 0.061538446 0.039037499 0.02640672 0.178600549
MIR-153-1,2 0.007463242 0.013054276 0.010169851 0.008351965 0.033556776 0.015329473
MIR-154 0.03810684 0.040433397 0.076242029 0.018287129 0.011788753 0.047374414
MIR-155 0.34355602 0.144719298 0.061952329 0.029434816 0.066867891 0.171104003
MIR-181a,c 0.295406867 0.174006635 0.229481828 0.113669682 0.122260766 0.292324554
MIR-181b-1,2 0.003320301 0.003466467 0.005242987 0.00469798 0.005529986 0.004434762
MIR-182 1.8092199 0.36510978 0.213300806 0.404927609 0.154220282 0.461689049
MIR-183 0.034626437 0.013416868 0.003729239 0.008781873 0.006127556 0.011673087
MIR-184 0.00650917 0.018151244 0.069584212 0.024543783 0.054876277 0.01811862
MIR-185 0.111836984 0.26769159 0.502010108 0.402068277 0.558487509 0.43384359
MIR-186-P 0.253484734 0.571298133 0.871751786 0.222153522 0.518808283 0.530816794
MIR-187 0.000503286 0.000357186 0.000993338
MIR-188b-P 0.000785985 0.0011076 0.000465485 0.000601966 0.002324476
MIR-190
MIR-191 0.000142585 0.003426759
MIR-192 0.001038721 0.003912362 0.005096535 0.000760393 0.005560321 0.001542617
MIR-193 0.022215482 0.004463101 0.019746158 0.00282715 0.002278155 0.033789491
MIR-194-1,2 0.000197003 0.00019211 0.002085518 0.001424343 0.000897184 0.000459821
MIR-195-P 0.025015884 0.06126635 0.256608403 0.068948808 0.175923537 0.096333068
MIR-196a-1 0.0025842 0.00253663 0.026677769 0.009978592 0.013863184 0.011072873
MIR-196a-2 0.007672252 0.020286408 0.082465833 0.008198623 0.036655079 0.047218546
MIR-196b 2.979555807 0.252353713 0.258893889 0.168122756 0.056456751 0.389062927
MIR-197 0.042846161 0.247770154 0.037168864 0.270062254 0.110716252
MIR-198 0.009788881 0.01326493 0.010915189 0.070886191 0.015912777
MIR-199a-1-P 0.005221231 0.035316954 0.410063949 0.126582899 0.377579782 0.042773347
MIR-199a-2-P 0.032327814 0.098385105 0.028148538 0.014269587 0.022799112 0.068797093
MIR-199b-P 0.027329877 0.030308573 0.084343975 0.018338379 0.10655834 0.043853762
MIR-200a-P 0.283689718 0.515488525 3.189947807 0.454580725 1.487552844 0.643583578
MIR-200b 0.020598824 0.013630655 0.039301994 0.032514745 0.011597936 0.050700891
MIR-200c 0.003775958 0.002015532 0.000977821 0.000249069 0.000318477 0.000835879
MIR-203 0.000934692
MIR-204 0.004495986 0.002774125 0.027315494 0.009332164 0.024473036 0.01480281
MIR-205 0.001038263 0.00210141 0.021473041 0.000283769 0.093822738 0.000404397
MIR-206 0.007976526 0.000320314 0.006888821 0.000670541 0.00438929
MIR-208 0.000170272 0.000983384 0.002823915 0.000327634 0.001136457
MIR-210 0.064105359 0.198336172 0.337325274 0.379640397 0.247647235 0.090546044
MIR-211 0.024666225 0.088092024 0.324387767 0.065680887 0.171249588 0.138424807
Continued
178
Table A. continued
MIR-212 1.734100809 0.251638351 0.299386862 0.243900466 0.021787023 0.339788968
MIR-213 0.172383043 0.047836287 0.089505294 0.057114957 0.027733748 0.186163858
MIR-214 0.005838752 0.020846106 0.091150717 0.048397855 0.097374878 0.037349362
MIR-215 0.003182246 0.012959871 0.032184901 0.013474303 0.018900771 0.019979114
MIR-216 0.006268814 0.006914431 0.010009143 0.002249678 0.013410756 0.005669772
MIR-217
MIR-218-1,2 0.091817074 0.035684682 0.041428613 0.013744921 0.016049253 0.068160603
MIR-219-1,2 0.004857408 0.009317029 0.003553838 0.009537877 0.012689956 0.002892494
MIR-220 0.004110083 0.000115645 0.001746064 0.000495082 0.005868884 0.000665098
MIR-221 0.228098722 0.091094245 0.198320857 0.074026104 0.086007806 0.061330262
MIR-222-P 0.140955558 0.037316984 0.025880464 0.019984897 0.011469926 0.022403958
MIR-223 0.00136571 0.00363094 0.001180088 0.001440352 0.000802095 0.001881075
MIR-224 0.011965282 0.035757625 0.031122577 0.010143008 0.031984504 0.047172011
MIR-296 0.00334642 0.000189265 0.001629796 0.007836955
MIR-299-P 0.000334262 0.002609252 0.035914726 0.007176307 0.029695753 0.009572082
MIR-301 0.064131403 0.047687046 0.054432791 0.036190213 0.084813188 0.074209794
MIR-302 0.007376687 0.010333911 0.002505876 0.005827179 0.006302511 0.010483167
MIR-302b 0.003009381 0.008773616 0.010203506 0.010700622 0.010286218 0.005688616
MIR-302c 0.000325725 0.003751419 0.002204114 0.00031053 0.008372136 0.003688501
MIR-302d 0.000313573 0.002101205 0.00221209 0.000464874
MIR-320 0.003101203 0.007169517 0.012966023 0.004122146 0.004543907 0.009680685
MIR-321 3.098310579 2.546448816 3.974356586 1.856676438 2.96830002 5.747712576
MIR-323 0.038619459 0.090303093 0.104044587 0.025876045 0.026635375 0.067894636
MIR-324-P 0.007975534 0.020212551 0.03895901 0.016909366 0.021689813 0.020786071
MIR-325 0.004211059 0.016226051 0.030837494 0.008372362 0.029178245 0.019742389
MIR-326-P 0.000328991 0.000429908 0.000197371 0.00037133 0.000707421
MIR-328 0.027043511 0.010072424 0.056335898 0.005480001 0.026340589 0.013101496
MIR-330 0.004129905 0.057564342 0.006762174 0.001432638 0.008400692 0.008882047
MIR-331-P 0.016453203 0.007779463 0.022774305 0.00422324 0.006723189 0.008870403
MIR-335 0.00070767
MIR-337 0.000109879 0.007463625 0.016655752 0.000354743 0.00227729 0.002048544
MIR-338 0.000624876 0.004451165 0.00881761 0.000356383 0.002253046 0.002279318
MIR-339 0.071945999 0.043825253 0.073350333 0.026386283 0.036534706 0.100194444
MIR-340 0.242215529 0.139297012 0.143854157 0.097001403 0.074171894 0.338029935
MIR-342 0.044457443 0.024027489 0.020423088 0.008155005 0.008306687 0.022786915
MIR-345 0.006488071 0.01858919 0.037761119 0.014685512 0.037042093 0.022758802
MIR-346 0.024943838 0.044033222 0.129453764 0.116897414 0.171418761 0.151873169
MIR-361 0.006717009 0.007929638 0.017574504 0.012526619 0.010567031 0.013935011
MIR-367 0.003931997 0.003504681 0.006263242 0.003507165 0.003840721
MIR-368 0.001574375 0.004425354 0.004797315 0.00377174 0.011859758
MIR-369 0.008518767 0.01407022 0.019133277 0.001249833 0.019324011
MIR-370 0.131031063 0.203429346 0.301997041 0.1087202 0.082204969 0.212060294
MIR-371 0.001305691 0.008552629 0.047106887 0.012208002 0.064540851 0.013701374
MIR-372
MIR-373 0.000677376 0.003713663 0.00964173 0.000378412 0.006322294 0.001460952
MIR-374-P 0.001036425 0.005261715 0.001164899
MIR-375 0.010471546 0.003388719 0.004931668 0.005542282 0.00444107 0.005042451
Continued
179
Table A. continued
MIR-376 0.621788039 0.961455652 0.63752765 0.216697951 0.160983577 0.576807736
MIR-377 0.017909244 0.013897982 0.011774445 0.000973283 0.00211521 0.011833018
MIR-378 0.000355776 0.000167964 0.000539144 0.000590746 0.000443352
MIR-379 0.361800867 0.314748895 0.356709445 0.126581188 0.080886285 0.229311151
MIR-380 0.114597682 0.065735236 0.09543669 0.011207983 0.008679135 0.065920702
MIR-381 0.005382904
MIR-382 0.000503092 0.013105032 0.008290006 0.000300506 0.000678756 0.002098368
MIR-383 0.000392014 0.000945061 0.001596799 0.000483469 0.001526976 0.000506535
MIR-384 0.000292133 0.005148446 0.000381008 0.00178303
MIR-422b 0.001244667 0.017058644 0.000608045 0.009283818 0.00757575
MIR-423 0.000210693 0.001415262 0.007030621 0.000708415 0.002881546 0.002672606
MIR-424 0.028173064 0.068299684 0.040104719 0.003977816 0.031530698 0.060103296
MIR-425 0.003850515 0.006943904 0.002634194 0.000219709 0.012170029
18S rRNA (CT) 6.7847897 6.89571015 7.61849425 7.23457885 7.40222035 7.14890985
Tissue Type Pancreatic Tumor
miRNAs OUP29(T) OUP37(T) OUP49(T) OUP101(T) OUP119(T) 0206C077B(T)
let-7a-1* 0.005190609 0.019623511 0.003227546 0.004840743 0.007383645 0.006690052
let-7a-2-P 0.038127954 0.407206083 0.049689959 0.116258872 0.208928668 0.377440612
let-7a-3* 0.001969118 0.04808016 0.004766276 0.008299529 0.013157488 0.011266257
let-7b* 0.009728148 0.091165168 0.014240614 0.012433176 0.025092967 0.031333084
let-7c* 0.002783066 0.001314505 0.001053485 0.00140158 0.002431742
let-7d* 0.045355692 0.069931173 0.042285612 0.038554477 0.051585248 0.069894298
let-7e* 0.001535123 0.001146975 0.00211911 0.000875175
let-7f-1* 0.070529075 0.100014114 0.054580207 0.051462656 0.099020763 0.093238724
let-7f-2* 0.007991821 0.001829775 0.0040218 0.003995694 0.00470112
let-7g* 0.02539987 0.010809743 0.007668925 0.01621097 0.023601647 0.028242069
let-7i* 0.042260464 0.085996188 0.033767617 0.025821296 0.044466291 0.093194658
MIR-001-1 0.0002544 0.005505491 0.000494027 0.000274864 0.000178149 0.000280116
MIR-001-2 0.109124044 0.074538341 0.090779048 0.029781665 0.117847525 0.097306978
MIR-007-1 0.049739573 0.017149259 0.028647969 0.007729912 0.020364961 0.01316299
MIR-007-2 0.000891226 0.000856129 0.000781181 0.000719643 0.002880158 0.002650389
MIR-007-3 0.000744681 0.00026217 0.275813823 0.052827125 0.673031703 0.886936712
MIR-009-1-P 0.110610321 0.12187837 0.091140252 0.032755244 0.081172163 0.105712367
MIR-009-2-P 0.072559404 0.131555778 0.06256832 0.02067369 0.050663546 0.048182033
MIR-009-3-P 0.003474616 0.000214548 0.000193969 0.007432694
MIR-010a,b 0.003757459 0.040425839 0.01506602 0.001195833 0.03801286 0.013890026
MIR-015a 0.010921296 0.074715274 0.026593116 0.004688968 0.041151943 0.025763967
MIR-015b 0.099645117 0.132851002 0.061040546 0.011962529 0.047807271 0.026761793
MIR-016-1 0.140006957 0.208287046 0.085861975 0.037565216 0.097333778 0.130061157
MIR-016-2 0.10268746 0.219125383 0.060563994 0.160868272 0.079899865 0.082778968
MIR-017-P 0.019504184 0.204083863 0.028559404 0.01072776 0.006482243 0.008380698
MIR-018 0.348734601 0.337874182 0.223818651 0.347720064 0.428829741 0.737244505
MIR-019a-P 0.039289426 0.139518807 0.018837805 0.048122694 0.031246564 0.031113652
MIR-019b-1-P 0.001438642 0.028035916 0.000243213 0.003023003
MIR-019b-2-P 0.000931787 0.000956506
Continued
180
Table A. continued
MIR-020 0.001198682 0.003833154 0.000885174 0.00040551 0.004116999
MIR-021 34.25400846 18.76520335 20.82220098 2.393053614 14.5300259 12.03163724
MIR-022-P 0.005918232 0.068903521 0.016998034 0.078240382 0.0202839 0.045954631
MIR-023a 0.105803659 0.246958584 0.085955107 0.043038414 0.070819102 0.130900687
MIR-023b-P 0.016958042 0.34063711 0.038576864 0.0730886 0.023125748 0.07360801
MIR-024-1,2 7.496719482 7.749668072 6.374734114 3.148811117 7.033724702 8.963695056
MIR-025 0.022896945 0.204638999 0.026981646 0.019044988 0.036817931 0.042508807
MIR-026a-1,2 0.003935454 0.009481296 0.017063 0.012921248 0.003090214 0.008232812
MIR-026b 0.086423168 0.112039396 0.107833524 0.103600633 0.058461103 0.057845321
MIR-027a, b 0.078799862 0.257413125 0.090297394 0.05640388 0.069804477 0.175987037
MIR-028 0.001740466 0.007598638 0.004308622 0.001257993 0.007344902
MIR-029a,c 0.067934556 0.037752317 0.023073756 0.019905276 0.017180899 0.086672376
MIR-029b-1,2 0.000339086
MIR-030a,e 0.048786149 0.086298185 0.034542125 0.01684911 0.008045514 0.023768474
MIR-030b 0.062626342 0.096236969 0.035588222 0.028703415 0.078220462 0.087719906
MIR-030c-1,2 0.037793696 0.077349888 0.016060083 0.011452765 0.035321303 0.041628246
MIR-030d 0.011491177 0.023820591 0.026423606 0.026175941 0.005994682 0.012371962
MIR-031-P 0.100288766 0.262323147 0.065718263 0.049346454 0.046529964 0.078961629
MIR-032 0.037866737 0.026628612 0.015114328 0.024350137 0.015330912 0.045920147
MIR-033 0.000223873 0.00097476 0.000284058
MIR-034a 0.025757001 0.056446581 0.017826245 0.008154249 0.024502306 0.048447132
MIR-034b 0.000700295 0.002073332 0.003953876 0.000369023 0.000376257 0.000538527
MIR-034c
MIR-092-1 0.006928516 0.007802262 0.006364699
MIR-092-2-P 0.003511438 0.0014085 0.000423229
MIR-093 0.029213397 0.098722245 0.018152873 0.006566841 0.012573311 0.017271606
MIR-095 0.002895011 0.000638801 0.024420697 0.000391646 0.014332875 0.000392524
MIR-096-P 0.161364812 0.430595611 0.242925639 0.094082142 0.058448219 0.04515536
MIR-098 0.947134304 0.973202897 1.160151062 0.425766463 1.244553604 1.44326485
MIR-099a 0.019101582 0.013804773 0.0140718 0.004592343 0.004946065 0.005596537
MIR-099b 0.40134892 0.310688559 0.476092455 0.175282725 0.281551135 0.293147583
MIR-100 0.211274041 0.428107204 0.265544245 0.101050245 0.538468318 0.807868884
MIR-101-1 0.002961118 0.001342611 0.000272736 0.000280868 0.000486862 0.001501755
MIR-101-2 0.046652507 0.114828767 0.076910995 0.139956628 0.047470593 0.089101194
MIR-103-1,2 0.405694717 0.57061557 0.510652463 0.085221498 0.404894477 0.626518306
MIR-105-1,2 0.005538728 0.00737148 0.004763688 0.00879835 0.00414743 0.009998342
MIR-106a-P 0.001006782 0.001621932 0.000248733
MIR-106b 0.1791513 0.441471901 0.168598391 0.024185903 0.129034152 0.087929317
MIR-107 0.941212848 1.151386354 1.010081126 0.232323053 0.986303713 0.981638385
MIR-108 0.001104128 0.032485957 0.007686839 0.000772301 0.006234832 0.010088224
MIR-122a 0.000601161 0.000338755
MIR-124a-1,2,3 0.001791065 0.002765688 0.007078759 0.000267038
MIR-125a 0.061475907 0.111372775 0.064563324 0.019929001 0.06914757 0.063029109
MIR-125b-1 0.17824888 0.147056914 0.141182352 0.173835449 0.306352041 0.835943632
MIR-125b-2 0.009280415 0.000722788 0.013408412 0.020055933 0.005538385
MIR-126 0.130255926 0.063859343 0.179187595 0.03702444 0.219193568 0.242719945
Continued
181
Table A. continued
MIR-127 1.484400528 1.85785965 2.045772429 1.492194111 1.069952959 1.01554669
MIR-128a 0.031267722 0.079543482 0.034319948 0.013608225 0.049869753 0.106473482
MIR-128b 0.004928695 0.008788731 0.008531057 0.003250506 0.002162373 0.001751919
MIR-129-2 0.067096807 0.242136256 0.065352406 0.036145033 0.011779906 0.025150826
MIR-130a-P 0.061839541 0.22541103 0.034280184 0.018910282 0.017735593 0.023566373
MIR-130b 0.001500851 0.004873544 0.000751554 0.000679396 0.000597797 0.002260342
MIR-132 0.011074312 0.011920595 0.011117939 0.022667243 0.016798135 0.029370967
MIR-133a-1,2 0.029171855 0.02653447 0.009347392 0.011819974 0.014615762 0.013232162
MIR-133b 0.004969662 0.045104792 0.010187181 0.005038628 0.007459144 0.019875598
MIR-134 0.067450639 0.118682203 0.061402137 0.024852016 0.033244255 0.089753597
MIR-135a-1,2 0.011163857 0.011145754 0.018611068 0.011315493 0.016060915 0.02613233
MIR-135b-P 0.025364498 0.102976784 0.031368235 0.000784901 0.00851511 0.00603735
MIR-136 0.017629776 0.018391864 0.035402748 0.009165989 0.627872647 0.029188706
MIR-137 0.006326062 0.001240787 0.00258976 0.003558575 0.004106527 0.00163034
MIR-138-1,2 0.000256382 0.000967204 0.000590038 0.000213566
MIR-139 0.039695586 0.085839324 0.010132793 0.009215092 0.013239146 0.006827961
MIR-140
MIR-141 0.024379797 0.016797706 0.010101772 0.009816526 0.004855319 0.003757123
MIR-142-P 0.054568811 0.037573687 0.009521425 0.006775611 0.015988793 0.035722183
MIR-143 0.041143103 0.039377546 0.02549462 0.008035083 0.046078701 0.024500858
MIR-144 0.000340943 0.000194942 0.000391057 0.000169911 0.000307827
MIR-145-P 0.207925113 0.304479292 0.12503238 0.105623717 0.297665385 0.241356832
MIR-146 0.005721858 0.002687878 0.001476033
MIR-147 0.016217519 0.001820846 0.008622107 0.001159237 0.0155992 0.012452309
MIR-148a-P 0.008807211 0.004260692 0.010296424 0.027419265 0.002453605 0.002168032
MIR-148b-P 0.005638792 0.098504915 0.02735182 0.031909653 0.045084204 0.074340081
MIR-149 0.041821016 0.116085446 0.045990467 0.017671805 0.014397927 0.017973523
MIR-150 0.03441369 0.039254722 0.038282097 0.009232815 0.024062951 0.026756414
MIR-151 0.010201523 0.01040586 0.008662335 0.000601256 0.000689154 0.005617956
MIR-152 0.015194841 0.058774351 0.017610866 0.013387099 0.021457047 0.011286735
MIR-153-1,2 0.007878452 0.010008364 0.025512767 0.009809387 0.00336995 0.026994112
MIR-154 0.00984527 0.016898658 0.011973014 0.026914692 0.015022918 0.062383684
MIR-155 0.081727642 0.058689553 0.048194946 0.015283425 0.041427552 0.159247769
MIR-181a,c 0.195881414 0.041271624 0.133374214 0.010416661 0.090044338 0.133507164
MIR-181b-1,2 0.007708412 0.011374221 0.005134209 0.002292759 0.003490386 0.003929164
MIR-182 0.715197659 0.183032852 0.495278592 0.041563536 0.105238308 0.07361676
MIR-183 0.045100052 0.009707507 0.013534751 0.004367593 0.010918904 0.001686843
MIR-184 0.027311851 0.068477793 0.022078878 0.016901765 0.019659976 0.021546795
MIR-185 0.494075499 0.350749273 0.705486152 0.541015457 0.313552813 0.314587242
MIR-186-P 0.562481643 0.870979712 0.403696552 0.395942467 0.573038458 0.442642557
MIR-187 0.003872087 0.000669155
MIR-188b-P 0.00107626 0.000885221 0.000274675 0.000479486 0.000267771 0.00020454
MIR-190 0.000163823 0.002418818 0.000206894
MIR-191 0.00127826
MIR-192 0.000465782 0.024213871 0.002387051 0.000501319 0.000291665 0.002367452
MIR-193 0.007527693 0.01855991 0.00057251 0.00251124 0.008512298 0.008577691
Continued
182
Table A. continued
MIR-194-1,2 0.001496059 0.002737784 0.000654082 0.000256153 0.000284806 0.000549224
MIR-195-P 0.137360387 0.273272752 0.128861363 0.033313084 0.061247462 0.074372371
MIR-196a-1 0.008171037 0.013876856 0.020576325 0.003345235 0.002927246 0.001238501
MIR-196a-2 0.026680581 0.062430508 0.005617472 0.072196308 0.016817838
MIR-196b 0.453012078 0.040426259 0.366569376 0.002109657 0.07247998 0.022554679
MIR-197 0.210408212 0.266344246 0.125610561 0.046727324 0.03972293 0.029588894
MIR-198 0.007105062 0.083157547 0.010905828 0.004856889 0.004341597 0.03062434
MIR-199a-1-P 0.30953746 1.222164532 0.768696588 0.825172827 0.159207408 0.08751712
MIR-199a-2-P 0.032431261 0.077353454 0.027146108 0.028835457 0.069276837 0.105692328
MIR-199b-P 0.057802469 0.160770991 0.07737518 0.014085135 0.027996298 0.043092768
MIR-200a-P 1.311363749 3.522129658 1.821912495 0.677648115 0.493509195 0.404491578
MIR-200b 0.027078853 0.0173219 0.047295826 0.021880966 0.003196334 0.02120144
MIR-200c 0.001014449 0.006108869 0.001194732 0.001233378 0.002618399 0.001483928
MIR-203 0.001404067
MIR-204 0.0091206 0.07692309 0.026637014 0.004619786 0.004513416 0.017811046
MIR-205 0.078338967 0.802624733 0.001323626 0.001752852 0.00834341 0.000892431
MIR-206 0.004511863 0.000179183 0.001901606
MIR-208 0.005701337 0.00132666 0.000603529 0.000333249 0.000508886
MIR-210 0.147353008 0.657721629 0.226861574 0.022569358 0.084295818 0.024982741
MIR-211 0.152433925 0.374236588 0.079315997 0.106724345 0.086729576 0.107526754
MIR-212 0.374247328 0.072225521 0.206226076 0.631667048 0.266144908 0.841938389
MIR-213 0.09421019 0.048527001 0.045657152 0.011913096 0.040649146 0.041556373
MIR-214 0.074213575 0.139634469 0.119461378 0.042010571 0.038571949 0.03406277
MIR-215 0.006499662 0.009180255 0.015106421 0.017175886 0.014011285
MIR-216 0.003528071 0.019598316 0.002926614 0.084536912 0.005283735 0.004980839
MIR-217 0.000689051
MIR-218-1,2 0.031779361 0.020618619 0.044638804 0.019362314 0.064471411 0.166662631
MIR-219-1,2 0.002538328 0.002100193 0.009224038 0.013575432
MIR-220 0.000501464 0.001492289 0.001805237 0.001532295 0.000325151 0.001622782
MIR-221 0.161433726 0.09394564 0.032224743 0.032882407 0.072310796 0.095200738
MIR-222-P 0.017272628 0.032556717 0.027655748 0.01781203 0.028232591 0.055230771
MIR-223 0.001000929 0.005271949 0.005754667 0.00023975 0.000238844 0.000614094
MIR-224 0.03325865 0.050340785 0.043138809 0.036399457 0.048762668 0.022543019
MIR-296 0.004824083 0.039016104 0.00118709 0.002042393 0.006701775 0.003253258
MIR-299-P 0.019769099 0.069138112 0.001770341 0.009962446 0.001115395 0.00506267
MIR-301 0.048498046 0.033301259 0.027041391 0.005245679 0.025413271 0.043455047
MIR-302 0.007969298 0.001694076 0.001175164 0.004820923 0.011628057 0.008726075
MIR-302b 0.012195907 0.015725482 0.019016238 0.013433371 0.007256992 0.009015037
MIR-302c 0.001334648 0.00448576 0.003846231 0.000438636 0.007442922 0.001301518
MIR-302d 0.00680626 0.000250321 0.000227788
MIR-320 0.017652269 0.014639149 0.005125735 0.00492222 0.004100879 0.007790775
MIR-321 1.333289823 2.770880551 1.899444646 0.897989648 2.634677484 1.73986886
MIR-323 0.027577738 0.048903839 0.04436249 0.041554606 0.047698753 0.100927769
MIR-324-P 0.030104812 0.038942964 0.033462039 0.016988888 0.014956183 0.017618227
MIR-325 0.026115161 0.037412314 0.005776934 0.00546184 0.02103173 0.012368189
MIR-326-P 0.000805373 0.00047485 0.000817109 0.000254828 0.000355397
Continued
183
Table A. continued
MIR-328 0.046246898 0.062345426 0.03438071 0.309404314 0.01134404 0.066085019
MIR-330 0.017082541 0.011241135 0.005832929 0.0008414 0.007572597 0.003904443
MIR-331-P 0.006654334 0.052383673 0.006772772 0.019607926 0.008511628 0.005850364
MIR-335 0.000178061
MIR-337 0.000852917 0.010751219 0.001372181 0.003720402 0.00462012
MIR-338 0.001122829 0.010599592 0.002687488 0.004858061 0.003025312 0.00291603
MIR-339 0.279239883 0.349018283 0.31886655 0.227095767 0.238741102 0.302493819
MIR-340 0.086971343 0.131609316 0.101426755 0.082402317 0.184531226 0.260496441
MIR-342 0.013799377 0.026200761 0.006408532 0.000702628 0.001868692 0.022003526
MIR-345 0.022259514 0.138467233 0.013055424 0.017578676 0.018288029 0.011513163
MIR-346 2.088388934 0.800109311 1.985488726 0.580464692 0.522643956 0.602309354
MIR-361 0.013321274 0.023841148 0.019682894 0.027925012 0.009957625 0.017845586
MIR-367 0.004086766 0.009473144 0.006236316 0.005012577 0.018772816 0.009228476
MIR-368 0.006224171 0.000908156 0.008908203 0.002494841 0.003868248
MIR-369 0.000524079 0.011952492 0.011321594 0.006458143
MIR-370 0.125828481 0.102021457 0.109038662 0.136238817 0.113931957 0.231580562
MIR-371 0.016477097 0.044126542 0.021318558 0.011342871 0.002805517 0.007352429
MIR-372 0.000362248
MIR-373 0.002173051 0.003063609 0.004324189 0.004388368 0.003187707 0.00184673
MIR-374-P 0.00491866 0.00025829
MIR-375 0.004505106 0.002259283 0.012703661 0.044787477 0.001657973 0.016360709
MIR-376 0.179836145 0.156697603 0.172329628 0.415869481 0.551318672 0.859904333
MIR-377 0.00154463 0.014921884 0.001945539 0.020150329 0.011231846 0.018646777
MIR-378 0.001602331 0.00138221 0.001314221 0.004003088 0.000231394
MIR-379 0.081108809 0.088753635 0.078844568 0.194429323 0.131021536 0.409344543
MIR-380 0.041974368 0.013828893 0.015633268 0.06770425 0.039316205 0.126424946
MIR-381 0.000226291 0.000416741
MIR-382 0.002432487 0.00942344 0.002685371 0.006875509 0.002141019 0.005676615
MIR-383 0.001153561 0.00635137 0.000754455 0.00131812 0.000176869 0.003106914
MIR-384 0.000596919 0.000388749 0.000269839
MIR-422b 0.003656171 0.016855457 0.003746491 0.000314766 0.000291427 0.001376291
MIR-423 0.004173199 0.007779489 0.001162248 0.00174003 0.001340675 0.001446238
MIR-424 0.010986506 0.008345999 0.003322422 0.015458179 0.026692206 0.07555076
MIR-425 0.006625752 0.00399033 0.006436585
18S rRNA (CT) 7.42572085 7.29846145 7.7145896 7.46156665 7.5298803 7.5777325
Tissue Type Pancreatic Tumor
miRNAs 0206C138B(T) 3030401A1(T) 3030800A3(T) 3030396A1(T) 1050005A2(T)
let-7a-1* 0.005133434 0.003980403 0.003588572 0.007406153
let-7a-2-P 0.067724306 0.036461071 0.067871915 0.101835662 0.202099418
let-7a-3* 0.0027362 0.004138353 0.002825296 0.007187211 0.005254625
let-7b* 0.014200077 0.036120064 0.00652169 0.032833236 0.029841184
let-7c* 0.001296226 0.000571947 0.001048809 0.003410567 0.001464899
let-7d* 0.044361379 0.044300238 0.010683169 0.051607848 0.039624666
let-7e* 0.002330971 0.003173779 0.001016213
let-7f-1* 0.05739057 0.090066803 0.021603517 0.073737191 0.058947587
Continued
184
Table A. continued
let-7f-2* 0.003214436 0.004087468 0.002061849
let-7g* 0.022313815 0.026479979 0.008009262 0.050434902 0.021374309
let-7i* 0.010710417 0.013920171 0.040476 0.067297922 0.061433635
MIR-001-1 0.000185864 0.001153697 0.006882548
MIR-001-2 0.107157298 0.132953095 0.051055264 0.110791781 0.046387071
MIR-007-1 0.013396594 0.016516769 0.000988303 0.004297732 0.014080512
MIR-007-2 0.003656055 0.003294781 0.004864514 0.001307652 0.000379573
MIR-007-3 0.53349495 0.151614619 0.84002171 0.373098191 0.182495471
MIR-009-1-P 0.083248554 0.171092244 0.011364654 0.024435158 0.100813522
MIR-009-2-P 0.050843654 0.105340511 0.011572646 0.007623321 0.051673112
MIR-009-3-P 0.000208292 0.005687138
MIR-010a,b 0.007838058 0.036153516 0.00480031 0.030721673
MIR-015a 0.002036612 0.018853312 0.019576376 0.040761354 0.031309354
MIR-015b 0.012348953 0.07410955 0.018484919 0.028097971 0.03522079
MIR-016-1 0.061886649 0.079434265 0.016048419 0.209230093 0.19073904
MIR-016-2 0.074056928 0.14150209 0.059219619 0.045478351 0.077775931
MIR-017-P 0.077356188 0.129454881 0.005759932 0.010224537 0.015322304
MIR-018 0.635436347 0.20012763 0.142691967 0.905631955 0.835042522
MIR-019a-P 0.16067212 0.045579022 0.002251513 0.000610185 0.030842052
MIR-019b-1-P 0.000269706 0.002018759 0.002199803 0.000836654
MIR-019b-2-P 0.000514895 0.000283057
MIR-020 0.001149537 0.000546007
MIR-021 7.059918452 5.288735295 6.270571861 27.81134803 25.16211119
MIR-022-P 0.051382398 0.067656543 0.014312386 0.004882434 0.033923999
MIR-023a 0.098726933 0.066388022 0.083639487 0.374299174 0.190266183
MIR-023b-P 0.081223979 0.105518493 0.008041409 0.014433758 0.051110006
MIR-024-1,2 5.526066867 4.913434315 2.972515884 26.88841425 9.075235426
MIR-025 0.021747125 0.066744037 0.007090905 0.029942682 0.014042081
MIR-026a-1,2 0.010227104 0.011032276 0.000944987 0.001062035 0.015271293
MIR-026b 0.07118892 0.106845109 0.042593778 0.035444812 0.077575428
MIR-027a, b 0.120185605 0.102356439 0.041785393 0.500900261 0.140819309
MIR-028 0.002501705 0.006366796 0.003453827 0.002257449 0.00029716
MIR-029a,c 0.048300821 0.061244146 0.017380791 0.072455714 0.039529063
MIR-029b-1,2 0.000250855
MIR-030a,e 0.016969131 0.030801091 0.003032602 0.001281762 0.010311222
MIR-030b 0.092656373 0.161932926 0.018912587 0.047815553 0.068134938
MIR-030c-1,2 0.027047865 0.030434517 0.010453755 0.039842259 0.048824347
MIR-030d 0.014283026 0.02732231 0.005915813
MIR-031-P 0.074932506 0.28572284 0.026278341 0.026160089 0.084416163
MIR-032 0.043981604 0.053500818 0.017411539 0.03415543 0.029994755
MIR-033 0.000198075 0.000217769 0.000211416
MIR-034a 0.02823966 0.093015004 0.004565357 0.030673153 0.061463344
MIR-034b 0.001168115 0.000825941 0.000344679 0.000509186
MIR-034c
MIR-092-1 0.006951167 0.00186516 0.004593812 0.004865847
MIR-092-2-P 0.002704837 0.021909848
Continued
185
Table A. continued
MIR-093 0.00388917 0.020397714 0.003054499 0.013815947 0.005461748
MIR-095 0.012538313 0.009456996 0.001271361 0.002037609
MIR-096-P 0.044343087 0.741084661 0.013738788 0.010632825 0.04503338
MIR-098 0.665067427 0.813998065 0.484337972 1.85252212 0.976102846
MIR-099a 0.00478524 0.002577298 0.003095662 0.00515806 0.007069699
MIR-099b 0.281366021 0.300048219 0.151934828 0.216656852 0.188814317
MIR-100 0.11912974 0.048838553 0.138879881 0.98025892 0.49795372
MIR-101-1 0.000949648 0.002790775 0.000239699 0.002136586
MIR-101-2 0.051854447 0.074882389 0.011944485 0.071704341 0.12253014
MIR-103-1,2 0.264510064 0.578820037 0.101264508 0.591232003 0.555660876
MIR-105-1,2 0.012632483 0.015088684 0.005728648 0.001913748 0.002636037
MIR-106a-P 0.000406344 0.000325471
MIR-106b 0.072825223 0.147488132 0.021052852 0.378509986 0.180477022
MIR-107 0.449040669 0.793146498 0.314224161 1.564766912 1.264302396
MIR-108 0.003615312 0.022703519 0.000660336 0.000436892 0.002225968
MIR-122a 0.003027487
MIR-124a-1,2,3 0.00736493 0.007949146 0.005037345 0.004510536
MIR-125a 0.042875566 0.052009492 0.015719373 0.120851582 0.079071772
MIR-125b-1 0.146649344 0.016371477 0.166565962 0.486989567 0.243003175
MIR-125b-2 0.000902296 0.005595133 0.013831283 0.012544803
MIR-126 0.066831266 0.071358335 0.113775239 0.460292477 0.217992978
MIR-127 1.742168323 1.776435453 0.721081143 0.359269724 0.732704595
MIR-128a 0.034823814 0.032421312 0.017142704 0.060452917 0.048565347
MIR-128b 0.003209529 0.010520126 0.003821411 0.001279039 0.001034149
MIR-129-2 0.023414232 0.216043356 0.002834036 0.006121459 0.017715163
MIR-130a-P 0.030384798 0.201631545 0.001161742 0.003852122 0.027757839
MIR-130b 0.000470602 0.013946581 0.000332304 0.00369559 0.00052755
MIR-132 0.023327112 0.008476041 0.06810448 0.09817461 0.049866874
MIR-133a-1,2 0.018503045 0.029893254 0.004880026 0.009463387 0.010844644
MIR-133b 0.004548681 0.047295698 0.006441822 0.002140901 0.006807645
MIR-134 0.039301536 0.12592763 0.015673058 0.029215915 0.036916871
MIR-135a-1,2 0.049037934 0.019368188 0.001443465 0.012758094
MIR-135b-P 0.007500401 0.068118726 0.00181632 0.082854655
MIR-136 0.025374637 0.000760031 0.033544267 0.051625146 0.016697548
MIR-137 0.001529667 0.004043845 0.000256484 0.000208251 0.000691625
MIR-138-1,2 0.001700043 0.001326426
MIR-139 0.005898286 0.04686726 0.000384512 0.000465882 0.003734112
MIR-140
MIR-141 0.006446387 0.002644701 0.00112953 0.00473075 0.002419915
MIR-142-P 0.014210548 0.039088208 0.000691913 0.00042035 0.016115894
MIR-143 0.014404601 0.033038174 0.012179794 0.010536469 0.052912268
MIR-144 0.000205069
MIR-145-P 0.039513735 0.09587904 0.026225081 0.007790487 0.229164515
MIR-146 0.007052113
MIR-147 0.001636001 0.001782 0.011373709
MIR-148a-P 0.003319025 0.004575077 0.002577803 0.001173523 0.002084363
Continued
186
Table A. continued
MIR-148b-P 0.017268404 0.099431706 0.005512634 0.006305899 0.035077374
MIR-149 0.021688544 0.030066052 0.001459487 0.003403049 0.027743278
MIR-150 0.026248677 0.06441595 0.016791047 0.034768985 0.028912478
MIR-151 0.000390508 0.005797014 0.002143134 0.00038253
MIR-152 0.012879108 0.009310115 0.019896349 0.025789306 0.036431989
MIR-153-1,2 0.000402803 0.018667704 0.000249464 0.020269148 0.02211369
MIR-154 0.014063958 0.015213256 0.061518591 0.021097028
MIR-155 0.01728457 0.024495003 0.019151551 0.125347947 0.149013771
MIR-181a,c 0.072811292 0.038602812 0.022991741 0.190192501 0.141687321
MIR-181b-1,2 0.008649896 0.00858242 0.002383363 0.001216357
MIR-182 0.191805636 0.340861431 0.014243145 0.621496542 0.260557117
MIR-183 0.006405074 0.015948911 0.000630222 0.008188999 0.014005294
MIR-184 0.01502821 0.088288159 0.006684394 0.007154875 0.008094707
MIR-185 0.387898478 0.366531798 0.27972954 0.176576073 0.198324054
MIR-186-P 0.434041537 0.534320683 0.046497083 0.020914854 0.629861297
MIR-187 0.001018437 0.001032328
MIR-188b-P 0.00051732 0.003991716 0.000334174 0.000176922
MIR-190 0.000131644
MIR-191
MIR-192 0.00392765 0.002466196 0.001903334 0.001777137
MIR-193 0.000632672 0.006792337 0.008818246 0.001957129 0.013948933
MIR-194-1,2 0.000564285 0.003288802 0.003126241 0.000244758
MIR-195-P 0.113982917 0.268037037 0.014028718 0.015097425 0.084560755
MIR-196a-1 0.008944596 0.031997916 0.004074153 0.009249766
MIR-196a-2 0.012494622 0.058818417 0.004522012 0.030699013
MIR-196b 0.128627589 0.870294009 0.012715306 0.188282167 0.03337612
MIR-197 0.060867293 0.581706677 0.002985747 0.021805476 0.071213944
MIR-198 0.038616066 0.029845263 0.003660028 0.000317785 0.008177772
MIR-199a-1-P 0.187336318 0.676234614 0.013107665 0.005886626 0.060755573
MIR-199a-2-P 0.006763288 0.027034458 0.023794889 0.001475803 0.051922371
MIR-199b-P 0.037678936 0.131216156 0.014612973 0.008295305 0.04063218
MIR-200a-P 0.593061868 9.898159357 0.098839363 0.15236382 0.415450376
MIR-200b 0.013768274 0.068792723 0.01161234 0.035155574 0.030538618
MIR-200c 0.010609037 0.004393416 0.007098808 0.000382971
MIR-203 0.004446687
MIR-204 0.006929214 0.038646572 0.001482941 0.008689894 0.012842408
MIR-205 0.000594056 0.00161093 0.0007031 0.00022595 0.000743754
MIR-206 0.005270811 0.000159631
MIR-208 0.000295565 0.001947902 0.001422938 0.000332861
MIR-210 0.064894927 0.402440183 0.01932195 0.038292755 0.467524077
MIR-211 0.113925533 0.242531286 0.035062982 0.012392493 0.090443527
MIR-212 0.60471699 0.043223637 1.613536283 2.752592926 0.618693838
MIR-213 0.006946979 0.002607694 0.00318656 0.078152767 0.080824196
MIR-214 0.062290243 0.133342656 0.030005744 0.009672166 0.03038799
MIR-215 0.007927032 0.024192694 0.006077755 0.016813036
MIR-216 0.053821784 0.007802998 0.003311114 0.001114756 0.003314349
Continued
187
Table A. continued
MIR-217
MIR-218-1,2 0.016590211 0.005959881 0.025820487 0.25448319 0.103025443
MIR-219-1,2 0.007534375 0.007584828 0.000503811 0.002456052
MIR-220 0.000314305 0.001419836 0.01530923 0.000500937
MIR-221 0.019540491 0.030082364 0.016909122 0.07809036 0.048878458
MIR-222-P 0.019462651 0.011043906 0.009277357 0.007292238 0.013609285
MIR-223 0.001878422 0.000713941
MIR-224 0.037116964 0.040680783 0.005497194 0.011878852 0.033868786
MIR-296 0.000550249 0.032697537 0.001220638 0.00037179
MIR-299-P 0.00960382 0.047632886 0.005999488 0.008632112 0.003551492
MIR-301 0.006077179 0.023034128 0.014709164 0.037592125 0.044428048
MIR-302 0.008741421 0.003152153 0.000474752
MIR-302b 0.016776534 0.022288453 0.005663282 0.001113922 0.004483787
MIR-302c 0.000880399 0.007049097
MIR-302d 0.000290718 0.00433254 0.000392968
MIR-320 0.005868675 0.020592043 0.00315622 0.003536285 0.007851699
MIR-321 1.863159286 1.138156755 5.177203472 3.505016581 3.022862945
MIR-323 0.008347781 0.01736615 0.028916636 0.098556803 0.045511504
MIR-324-P 0.023062089 0.062842647 0.006043123 0.007497513 0.01719757
MIR-325 0.006720909 0.007139218 0.005650405 0.011242937 0.021111349
MIR-326-P 0.001140307 0.000423802 0.0025738
MIR-328 0.135299369 0.114523119 0.005052671 0.003774015 0.010919246
MIR-330 0.000267543 0.018177741 0.002583705 0.007034458
MIR-331-P 0.0163592 0.038865187 0.002931579 0.004649203 0.004425099
MIR-335 0.000374453
MIR-337 0.009114783 0.032840827 0.009765059 0.005351827 0.001614225
MIR-338 0.000399859 0.033035346 0.000240558 0.003445407 0.005257115
MIR-339 0.278673267 0.204247634 0.138118791 0.125824151 0.215747698
MIR-340 0.113907766 0.134223275 0.083617199 0.143854252 0.150457932
MIR-342 0.001610873 0.014855045 0.00657685 0.023631521 0.0167211
MIR-345 0.025482913 0.097940425 0.001370104 0.001061799 0.011535878
MIR-346 0.695879069 0.807154228 0.535144542 0.325263033 0.423613382
MIR-361 0.024530059 0.02535974 0.018478552 0.010103635 0.013820878
MIR-367 0.008643849 0.002338299 0.003248284
MIR-368 0.004417447 0.002818706 0.001084104 0.003028076
MIR-369 0.021602177
MIR-370 0.116781449 0.019463565 0.052653747 0.226491886 0.109222468
MIR-371 0.004066243 0.037174155 0.003501078 0.005018484 0.001924185
MIR-372
MIR-373 0.005607757 0.00323157 0.006648237 0.007168144 0.001573446
MIR-374-P 0.0112507 0.001301029
MIR-375 0.040484908 0.014356519 0.008896268 0.025434037 0.004616719
MIR-376 0.25573429 0.071457204 0.095333364 0.748915716 0.231732945
MIR-377 0.009052823 0.002112221 0.010919401 0.000940251 0.001911933
MIR-378 0.003330636 0.000586207
MIR-379 0.126584048 0.02556935 0.052139191 0.211128504 0.110762511
Continued
188
Table A. continued
MIR-380 0.036233627 0.008850289 0.016818181 0.075804341 0.021945768
MIR-381 0.005881141
MIR-382 0.000528506 0.000666256 0.001181835 0.004237192 0.001681083
MIR-383 0.000522747 0.002064121 0.001005945
MIR-384 0.001201817 0.00031384
MIR-422b 0.001292978 0.025362526 0.000857448 0.004619174
MIR-423 0.001280638 0.005961579 0.002559155 0.002873356
MIR-424 0.017288159 0.018592113 0.037290715 0.058462368
MIR-425 0.004472042 0.005050771 0.006491324
18S rRNA (CT) 7.43077495 7.5112732 7.71568895 7.4559257 6.83367
Tissue Type Pancreatic Cancer Cell Lines
miRNAs Panc-1 HS766T MIA PaCa-2 HPAF-II BxPC-3
let-7a-1* 0.014689983 0.029424063 0.026016543 0.021891226 0.054560029
let-7a-2-P 1.215614736 0.951875153 0.170737643 0.218718411 0.382668146
let-7a-3* 0.016761953 0.007213141 0.006991831 0.011108887 0.005784802
let-7b* 0.060569406 0.023890217 0.004356528 0.006321863 0.002151561
let-7c* 0.000224922
let-7d* 0.052034005 0.128278542 0.082853392 0.102022769 0.152568361
let-7e* 0.001029079 0.002531811
let-7f-1* 0.050880532 0.102415662 0.05535581 0.063213218 0.094124116
let-7f-2* 0.006086532 0.001937428 0.003713729 0.009928174 0.003426986
let-7g* 0.026948786 0.041999072 0.027204065 0.040502986 0.032870386
let-7i* 0.035329021 0.075670814 0.062553051 0.055809211 0.060615086
MIR-001-1 0.084373489 0.074377407 0.038418142 0.052467308 0.043130634
MIR-001-2 0.114839795 0.11923323 0.15254339 0.078857242 0.089589329
MIR-007-1 0.033656817 0.04559057 0.043051702 0.012926357 0.048478293
MIR-007-2 0.063038677 0.055770413 0.014865544 0.008825643 0.016855891
MIR-007-3 0.01656259 0.013061064 0.004597732 0.005322429 0.005645176
MIR-009-1-P 1.965336801 1.113472764 0.454012546 0.209237025 0.599450993
MIR-009-2-P 2.510819936 2.186238461 0.358302514 0.644507061 0.5289729
MIR-009-3-P 0.030135098 0.03161609 0.002268181 0.002355114 0.004251714
MIR-010a,b 0.178421647 0.020252717 0.016980739
MIR-015a 0.132661539 0.109618847 0.028228085 0.003701092 0.031254965
MIR-015b 0.227935662 0.141716512 0.434778369 0.127119219 0.358185222
MIR-016-1 0.115393396 0.15607315 0.195903974 0.008982745 0.029782996
MIR-016-2 0.416784511 0.406453515 0.176315727 0.179994418 0.143718403
MIR-017-P 0.614890776 0.736865273 0.452785941 0.300975627 0.258729367
MIR-018 0.421903678 1.901870835 1.277508216 0.298697707 0.351443145
MIR-019a-P 0.147038776 0.73395869 0.327244209 0.086583718 0.073681958
MIR-019b-1-P 0.102008945 0.484021242 0.11768722 0.106247262 0.126697479
MIR-019b-2-P 0.002367534 0.000939725 0.000633613 0.000516091
MIR-020 0.000896613 0.005801776 0.006423023 0.000710354 0.002237857
MIR-021 3.760822115 9.582149147 1.972360915 0.599660383 0.91993971
MIR-022-P 0.264434363 0.210206731 0.37645402 0.490434906 0.528786855
MIR-023a 0.416988664 0.38517014 0.109734195 0.174904652 0.117583475
Continued
189
Table A. continued
MIR-023b-P 0.486750906 0.546206644 0.169812677 0.458434514 0.354744868
MIR-024-1,2 1.578850499 1.155728696 1.265184748 0.97456078 0.842553802
MIR-025 0.208011842 0.348605629 0.336704421 0.223547462 0.502601966
MIR-026a-1,2 0.071196596 0.065274521 0.019025228 0.024919641 0.036988631
MIR-026b 0.699443326 0.700339454 0.136718234 0.274594012 0.305880113
MIR-027a, b 0.507591589 0.574960707 0.165823637 0.181005014 0.223834251
MIR-028 0.020922594 0.008321381 0.005522501 0.009275158 0.006113161
MIR-029a,c 0.117629887 0.715769398 0.295527967 0.165642188 0.097988226
MIR-029b-1,2 0.008302405 0.014061451 0.000427962 0.000708616 0.005549684
MIR-030a,e 0.15955172 0.119571359 0.257736107 0.100822817 0.098816839
MIR-030b 0.44866796 0.549599947 0.126600374 0.139861624 0.253051638
MIR-030c-1,2 0.390038264 0.209342519 0.0505533 0.060380084 0.109786283
MIR-030d 0.007240289 0.009241507 0.017006606 0.00041239 0.027582398
MIR-031-P 2.057803277 1.936402376 0.765265396 1.067807117 0.974132362
MIR-032 0.024433402 0.088664093 0.108435539 0.015955367 0.035594934
MIR-033 0.011418204 0.005042666 0.000995876 0.004200039 0.003459053
MIR-034a 0.048000794 0.132358318 0.041085997 0.130966893 0.144220507
MIR-034b 0.038106266 0.049876577 0.0066778 0.013846821 0.011676791
MIR-034c
MIR-092-1 0.012733025 0.026810311 0.028767339 0.010579106 0.01232452
MIR-092-2-P 0.215627647 0.145450965 0.022954647 0.0584104 0.065666584
MIR-093 0.338061603 0.31416193 0.104214366 0.141346451 0.218977223
MIR-095 0.004024628 0.003257062 0.001209885 0.002364665 0.002491766
MIR-096-P 9.448907745 8.907741976 2.852457852 3.808336791 4.079556212
MIR-098 1.788369776 2.601441508 0.92821928 1.348737949 1.056971678
MIR-099a 0.024845684 0.023788412 0.004336244 0.002090208 0.002820906
MIR-099b 0.149972467 0.140219925 0.317633959 0.152413919 0.297243099
MIR-100 0.87253419 1.706741739 0.003376366 0.005179794 0.001180531
MIR-101-1 0.01979219 0.010725657 0.00399536 0.005549358 0.005533284
MIR-101-2 0.082452273 0.173813346 0.063388703 0.069489416 0.038273409
MIR-103-1,2 1.454256819 1.633027708 0.709262251 0.767925132 0.858795013
MIR-105-1,2 0.084867681 0.08865383 0.024232475 0.061406346 0.023827596
MIR-106a-P 0.04143455 0.04303069 0.004276059 0.007400803 0.011686354
MIR-106b 0.318227533 0.874255135 0.255560375 0.175975998 0.620078262
MIR-107 1.167490709 1.737092127 1.137013646 0.689367203 0.990919124
MIR-108 0.147506156 0.104290581 0.053001062 0.042003652 0.080452341
MIR-122a 0.046206899 0.034778639 0.001876223 0.003857975 0.008251306
MIR-124a-1,2,3 0.046255687 0.04093469 0.005467568 0.011906941 0.011735814
MIR-125a 0.34411366 0.119008066 0.051784713 0.084178585 0.179638965
MIR-125b-1 0.115293179 0.05919954 0.005196338 0.00382292 0.004344772
MIR-125b-2 0.036297842 0.054503484 0.018430826 0.012933007 0.015347987
MIR-126 0.026105382 0.069834065 0.026173906 0.018848004 0.007130457
MIR-127 2.991003439 2.85193731 1.159040974 0.927787343 0.989493558
MIR-128a 0.10598989 0.082129611 0.095090272 0.070300734 0.052004426
MIR-128b 0.059616503 0.042031544 0.017719148 0.01749488 0.015117734
MIR-129-2 3.363711724 2.533240886 1.104991345 1.278370985 1.718137168
Continued
190
Table A. continued
MIR-130a-P 4.292721475 2.960371576 0.780075987 1.004394177 1.208414348
MIR-130b 0.040522207 0.036931298 0.0187605 0.020251633 0.015256478
MIR-132 0.004581836 0.000275392 0.002125923 0.001520406 0.003940443
MIR-133a-1,2 0.260091484 0.246877587 0.104452474 0.119490603 0.09496612
MIR-133b 0.655079574 0.354244723 0.166671816 0.205001102 0.255693204
MIR-134 1.355103052 1.915921922 0.110267655 0.231476729 0.303297649
MIR-135a-1,2 0.038550184 0.045445784 0.014733087 0.041729431 0.047340286
MIR-135b-P 0.135599012 0.051358247 0.02634575 0.061940274 0.046744444
MIR-136 0.041367084 0.020137666 0.02994192 0.005384869 0.006809345
MIR-137 0.006967067 0.00378194 0.262315156 0.001022685 0.004195416
MIR-138-1,2 0.022761289 0.031557665 0.004548506 0.005390234 0.013908231
MIR-139 0.990850682 0.837172757 0.138702103 0.199450075 0.213728502
MIR-140 0.000494714 0.000790884 0.000185383
MIR-141 0.047453443 0.021268208 0.013292222 0.014359502 0.01846378
MIR-142-P 1.84510839 1.72689668 0.269200234 0.316402756 0.370671916
MIR-143 0.146796306 0.121602835 0.045282316 0.078702216 0.090456546
MIR-144 0.004273995 0.000211521 0.001611783 0.000196573
MIR-145-P 0.279932824 0.19608088 0.03118981 0.049227783 0.073245134
MIR-146 0.008739174 0.002933663 0.001122817 0.001402907 0.003093878
MIR-147 0.06800634 0.057225353 0.016070304 0.008937955 0.007352174
MIR-148a-P 0.034389253 0.035980568 0.01310991 0.020190355 0.008971872
MIR-148b-P 0.160079899 0.116090938 0.054532385 0.098589347 0.100347336
MIR-149 0.850694817 0.798509662 0.211119474 0.186259262 0.305890291
MIR-150 0.252732494 0.263711843 0.110007674 0.108335545 0.13836186
MIR-151 0.076475454 0.114664068 0.012730502 0.025198158 0.047565446
MIR-152 0.03298394 0.049564699 0.004510791 0.003247903 0.021062109
MIR-153-1,2 0.023889021 0.042377411 0.016159457 0.003848826 0.010305503
MIR-154 0.036697614 0.027289699 0.001752782 0.002042525 0.003501138
MIR-155 0.032031431 0.180881626 0.00901883 0.033454233 0.033461971
MIR-181a,c 0.111326509 0.077604266 0.029614213 0.039796147 0.034017884
MIR-181b-1,2 0.059067365 0.022395129 0.007794791 0.038861963 0.034669749
MIR-182 0.969931703 1.538739241 2.387781077 0.532845457 0.925524906
MIR-183 0.064979077 0.035714761 0.027999581 0.014641798 0.016832949
MIR-184 0.789539894 0.65826882 0.331164187 0.317596423 0.347606923
MIR-185 1.592553202 1.452835408 0.619911456 0.835331397 0.412348544
MIR-186-P 0.887603689 0.958754779 0.802428954 0.828483327 0.908724713
MIR-187 0.056302296 0.021898354 0.009822807 0.033257973 0.050340268
MIR-188b-P 0.090124369 0.093399509 0.010901066 0.015880285 0.006715506
MIR-190 0.002650681 0.000683253 0.000248049 0.00023973
MIR-191 0.027667777 0.01048616 0.001033269 0.005814767 0.011002805
MIR-192 0.238862961 0.213643565 0.074683848 0.104687063 0.097302266
MIR-193 0.130988455 0.089787491 0.024510336 0.01919638 0.012208246
MIR-194-1,2 0.034644114 0.031304878 0.006601834 0.008154661 0.011534184
MIR-195-P 4.045403391 1.463522849 0.78508843 0.993658536 1.243551899
MIR-196a-1 0.183169356 0.162622273 0.065926992 0.023597586 0.077905539
MIR-196a-2 0.144371669 0.06813804 0.0331909 0.021441046 0.044720735
Continued
191
Table A. continued
MIR-196b 0.567789104 2.190982209 0.071559696 0.372834965 0.768942017
MIR-197 2.886561712 8.189062333 1.49356401 2.320790137 2.539767123
MIR-198 0.013737255 0.000825324 0.010110881 0.037196239 0.030077943
MIR-199a-1-P 1.763449876 1.834023684 0.575726119 1.387842228 1.08485725
MIR-199a-2-P 0.160294804 0.160347389 0.050400018 0.092224672 0.051054333
MIR-199b-P 1.432595256 1.335762733 0.499945095 0.65931649 0.73065979
MIR-200a-P 33.89899434 37.57524926 15.35388741 21.84227266 22.08802038
MIR-200b 0.052592525 0.04161224 0.029261267 0.038537013 0.047395593
MIR-200c 0.077414953 0.053718032 0.006388538 0.012474161 0.011491992
MIR-203 0.012564025 0.012226263 0.000366726 0.019008237 0.005788181
MIR-204 0.91047264 1.174366948 0.178871082 0.136685259 0.297152561
MIR-205 0.007647747 0.012209909 0.001356812 0.003122242 0.006691474
MIR-206 0.098836523 0.063932188 0.017067506 0.020759789 0.023259949
MIR-208 0.059670951 0.050966065 0.013181479 0.008854049 0.018139063
MIR-210 8.069417694 11.23286292 0.793942026 1.336135729 2.29606617
MIR-211 1.785009892 1.479762116 0.635122845 0.412873884 1.056524864
MIR-212 0.451087299 0.934878166 0.112668964 0.057055749 0.136171455
MIR-213 0.120233103 0.131966191 0.011870171 0.014735763 0.021443619
MIR-214 1.025481218 1.043302319 0.283351073 0.290716844 0.451570523
MIR-215 0.024187544 0.041476135 0.051919708 0.035676368 0.02355323
MIR-216 0.149841959 0.103966797 0.047588856 0.051919528 0.035701988
MIR-217 0.003877702 0.004875057 0.002679534 0.001148097 0.002268328
MIR-218-1,2 0.018270679 0.01369654 0.005678606 0.001269134 0.005688168
MIR-219-1,2 0.017816436 0.033019058 0.003351322 0.014872284 0.007248844
MIR-220 0.153397999 0.145895245 0.08823957 0.030933172 0.049256493
MIR-221 0.051986779 0.26248698 0.198088642 0.194905346 0.183702189
MIR-222-P 0.104068732 0.167242193 0.184939855 0.141519972 0.099790357
MIR-223 0.023883425 0.0438661 0.01085596 0.002493738 0.0047137
MIR-224 0.082441557 0.083705386 0.044308078 0.069132867 0.06054962
MIR-296 0.105960985 0.006719787 0.017487757 0.007425259 0.047182992
MIR-299-P 0.675010603 0.536266958 0.079293838 0.121553941 0.175746422
MIR-301 0.057561642 0.124495351 0.043526628 0.005133029 0.026028279
MIR-302 0.017007755 0.024180339 0.002229517 0.006394428 0.016328502
MIR-302b 0.180730584 0.175827821 0.023349348 0.055298305 0.059011695
MIR-302c 0.085237794 0.085136186 0.018194769 0.04122325 0.036834277
MIR-302d 0.041030031 0.074029945 0.020333594 0.015939151 0.017445935
MIR-320 0.245108695 0.244671466 0.057031412 0.050273574 0.07436676
MIR-321 4.858343745 2.965308631 0.935444567 0.87030173 0.907855265
MIR-323 0.057981203 0.101093964 0.029696013 0.047848336 0.027498138
MIR-324-P 0.38600771 0.294014104 0.078050845 0.100470723 0.112827193
MIR-325 0.044254472 0.016023418 0.01091732 0.01427224 0.018499688
MIR-326-P 0.063360326 0.051234796 0.005800438 0.006009989 0.005938992
MIR-328 1.195419573 1.758591949 0.292340288 0.443862142 0.67912414
MIR-330 0.227123292 0.178744192 0.049171053 0.032752199 0.082701982
MIR-331-P 0.178113845 0.221228824 0.084231465 0.09833161 0.078404932
MIR-335 0.002140884 0.005067129 0.011269436 0.002848179 0.007332281
Continued
192
Table A. continued
MIR-337 0.325058113 0.281893627 0.049669863 0.0852297 0.128232843
MIR-338 0.530496842 0.379431476 0.043799314 0.060879067 0.075029524
MIR-339 0.738687756 0.406190885 0.213998275 0.153064812 0.20964869
MIR-340 0.115483974 0.177133903 0.19547284 0.110320635 0.098423915
MIR-342 0.203630359 0.173455774 0.053469873 0.047276812 0.133599288
MIR-345 1.625364815 1.735458985 0.365907672 0.501684256 0.425251792
MIR-346 1.265900988 1.193876222 1.214366645 0.608903947 1.383527403
MIR-361 0.073122924 0.058831118 0.025912636 0.036607158 0.036216971
MIR-367 0.001535133 0.005162706 0.022811033 0.009574187
MIR-368 0.002261099 0.001432198 0.000723483 0.000538328
MIR-369 0.004093066 0.000935518 0.00996237
MIR-370 0.210093156 0.109012333 0.019887263 0.022520881 0.027865411
MIR-371 1.011833261 0.709777511 0.132356327 0.2249251 0.244906797
MIR-372 0.004742757 0.003843257 0.000728109 0.002007003
MIR-373 0.213937615 0.191986899 0.01783599 0.055384614 0.069419689
MIR-374-P 0.023350448 0.031589968 0.002495027 0.010228498 0.005226204
MIR-375 0.002678673 0.040655639 0.011090591 0.014533662 0.013451676
MIR-376 0.245514829 0.011812865 0.001363119 0.016815245 0.007531802
MIR-377 0.164414115 0.139027508 0.039130519 0.034602668 0.055716202
MIR-378 0.047020708 0.075951948 0.013409703 0.038895568 0.017880938
MIR-379 0.104241422 0.001057981 0.003631251 0.002226923 0.023643783
MIR-380 0.046779737 0.006165358 0.00311734 0.015082596 0.008442844
MIR-381 0.031302335 0.017096488 0.000278593 0.00513895
MIR-382 0.020188689 0.008611324 0.003163937 0.004260514 0.002420862
MIR-383 0.072073543 0.061676588 0.002356651 0.010283595 0.012549004
MIR-384 0.00090068 0.000585526 0.000158037 0.00141642 0.000503453
MIR-422b 0.283622731 0.272515136 0.083057086 0.151554707 0.193058398
MIR-423 0.157948273 0.128951125 0.017209274 0.047172098 0.05108644
MIR-424 0.011412503 0.027615944 0.001305959 0.001597745 0.005569692
MIR-425 0.039172094 0.010394677 0.021429865 0.028883344 0.029431252
18S rRNA (CT) 7.343109 7.3157717 7.3665025 7.807472 7.29358315
Tissue Type Pancreatic Cancer Cell Lines
miRNAs Mpanc-96 PL45 Panc03.27 Panc10.05
let-7a-1* 0.036952533 0.046147461 0.050414918 0.058756905
let-7a-2-P 0.168434441 0.506043415 0.631018199 0.268278848
let-7a-3* 0.038356385 0.033472766 0.010069662 0.016953198
let-7b* 0.008537471 0.00425231 0.00175498 0.006474351
let-7c* 0.000174829
let-7d* 0.166182235 0.168128542 0.147753122 0.131633374
let-7e* 0.001230644 0.002630083 0.001846209
let-7f-1* 0.133895703 0.145748311 0.155049956 0.164683363
let-7f-2* 0.009513738 0.027067737 0.004253808 0.006391174
let-7g* 0.053536293 0.029966223 0.053932511 0.05206843
let-7i* 0.171302792 0.025608562 0.024245293 0.02795596
MIR-001-1 0.016879583 0.034339624 0.018673032 0.053395657
Continued
193
Table A. continued
MIR-001-2 0.062011262 0.097926903 0.286958402 0.126871218
MIR-007-1 0.039219123 0.046464367 0.083247631 0.069763669
MIR-007-2 0.009033827 0.012699479 0.070005545 0.026587247
MIR-007-3 0.000854203 0.004095028 0.015011527 0.009415688
MIR-009-1-P 0.267408841 0.524550679 1.541344682 0.61424909
MIR-009-2-P 0.205097466 0.583987976 1.583642312 0.896325856
MIR-009-3-P 0.001665989 0.008048949 0.015446042 0.008927851
MIR-010a,b 0.063408786 0.060663122 0.02119504 0.025974652
MIR-015a 0.045213865 0.04149298 0.068289852 0.063560894
MIR-015b 0.188753666 0.574116558 1.055935724 0.479453305
MIR-016-1 0.115963892 0.140884038 0.125929834 0.173592704
MIR-016-2 0.098097492 0.324663604 0.359161227 0.204928099
MIR-017-P 0.61982939 0.42247696 0.8038354 0.473829432
MIR-018 1.099089651 0.426470563 0.950038918 0.785650308
MIR-019a-P 0.2823961 0.133210856 0.437269013 0.275277714
MIR-019b-1-P 0.098420324 0.123493804 0.381636224 0.071499686
MIR-019b-2-P 0.000321462 0.000738694 0.000289451
MIR-020 0.005703424 0.011159178 0.008057201 0.001427686
MIR-021 3.491355029 1.491954015 2.121551234 4.335253089
MIR-022-P 1.058946402 0.123116119 0.376091738 0.149210268
MIR-023a 0.254987348 0.166226012 0.34865994 0.241387641
MIR-023b-P 0.279687575 0.340818386 0.584881199 0.270575332
MIR-024-1,2 4.491900806 0.994272058 1.065213427 2.271743923
MIR-025 0.289961964 0.30807768 0.411187559 0.394759727
MIR-026a-1,2 0.043420949 0.040929563 0.089796188 0.059387052
MIR-026b 0.154611128 0.291118629 0.624139541 0.32750032
MIR-027a, b 0.214589006 0.150052324 0.450931131 0.304433435
MIR-028 0.000937392 0.01202717 0.026102296 0.010506808
MIR-029a,c 0.263219553 0.390939447 0.125706227 0.305149413
MIR-029b-1,2 0.001590337 0.000888185 0.00303903 0.000553909
MIR-030a,e 0.044135614 0.169293275 0.303131991 0.105450637
MIR-030b 0.157991634 0.19505151 0.405413917 0.293680365
MIR-030c-1,2 0.049027876 0.09418183 0.130357367 0.106800234
MIR-030d 0.051848842 0.03095123 0.032656818 0.034803223
MIR-031-P 0.304943119 0.365363664 2.686196826 1.579659067
MIR-032 0.065948816 0.085047968 0.107992013 0.110787208
MIR-033 0.002038838 0.00306635 0.00808488 0.004183629
MIR-034a 0.030340014 0.068892301 0.13728758 0.098841055
MIR-034b 0.002470559 0.006352009 0.025357108 0.036689059
MIR-034c
MIR-092-1 0.033720484 0.014452928 0.026446993 0.030317019
MIR-092-2-P 0.007329335 0.02242087 0.118182434 0.054368383
MIR-093 0.232766214 0.445634573 0.224746962 0.369011509
MIR-095 0.00026051 0.004281519 0.005365045 0.011006196
MIR-096-P 1.831185024 4.815274588 7.150509383 6.912206026
MIR-098 0.881466147 2.490519262 2.641559707 1.560396556
Continued
194
Table A. continued
MIR-099a 0.002548339 0.008901521 0.004835492 0.007042375
MIR-099b 0.256197902 0.74593435 0.425435262 0.293184394
MIR-100 0.061221388 0.111523958 0.224371295 0.202721951
MIR-101-1 0.007186142 0.00727429 0.014404252 0.021762049
MIR-101-2 0.049928264 0.054892736 0.174582096 0.060871563
MIR-103-1,2 0.614871383 1.047206218 1.602468524 1.190551657
MIR-105-1,2 0.021782986 0.076577882 0.087970951 0.042180579
MIR-106a-P 0.006021247 0.00730393 0.040726214 0.014797423
MIR-106b 0.337005506 0.207592765 0.54659627 0.392770156
MIR-107 1.015961385 1.088310007 1.145563967 1.037616783
MIR-108 0.027474843 0.161451256 0.157564619 0.057923967
MIR-122a 0.002018879 0.006303953 0.023069372 0.011620188
MIR-124a-1,2,3 0.004996954 0.007322967 0.045709822 0.017903168
MIR-125a 0.052313644 0.115595654 0.194473437 0.173714155
MIR-125b-1 0.000804773 0.014539955 0.023166157 0.030171176
MIR-125b-2 0.004123797 0.053592932 0.047543108 0.026405087
MIR-126 0.004871651 0.008577804 0.025283798 0.0338066
MIR-127 1.096190905 1.837541801 1.702771702 1.483041757
MIR-128a 0.064635814 0.197238429 0.128821207 0.197510153
MIR-128b 0.011056063 0.014506382 0.039473548 0.019889947
MIR-129-2 0.531237564 1.705443629 2.76603233 1.658259418
MIR-130a-P 0.406529533 1.136378601 2.629150119 1.459585495
MIR-130b 0.019142082 0.003902893 0.047719708 0.029610789
MIR-132 0.004356065 0.0007963 0.004118003 0.002465293
MIR-133a-1,2 0.076996307 0.086714861 0.301318993 0.189432033
MIR-133b 0.129994913 0.234164303 0.380307295 0.33873682
MIR-134 0.075252669 0.238135658 0.771499977 0.298946084
MIR-135a-1,2 0.097811443 0.005371444 0.054747979 0.03914037
MIR-135b-P 0.013633432 0.07573007 0.085506122 0.084167304
MIR-136 0.001334299 0.002605869 0.01301952 0.007236146
MIR-137 0.001628188 0.01065173 0.007767524 0.007860235
MIR-138-1,2 0.00098652 0.000869442 0.037960124 0.006028892
MIR-139 0.075996304 0.14764824 0.501924933 0.253455736
MIR-140
MIR-141 0.013502558 0.032151126 0.036954197 0.045835103
MIR-142-P 0.127289566 0.492307482 0.750806747 0.471383191
MIR-143 0.033316763 0.062052067 0.163513598 0.095378818
MIR-144 0.001879816
MIR-145-P 0.022719156 0.038725417 0.095554228 0.068384716
MIR-146 0.001585507 0.002184824 0.005301194 0.004697727
MIR-147 0.000793618 0.025616001 0.029463508 0.028573859
MIR-148a-P 0.009562732 0.022384611 0.027245221 0.018279067
MIR-148b-P 0.070475351 0.063615762 0.170614896 0.149152516
MIR-149 0.085460056 0.182809072 0.683557933 0.26962669
MIR-150 0.059042252 0.199816215 0.27137656 0.117294252
MIR-151 0.025995723 0.02407893 0.079652697 0.041989174
Continued
195
Table A. continued
MIR-152 0.010908606 0.013413417 0.01066396 0.032411806
MIR-153-1,2 0.116845864 0.002953036 0.008482951 0.010924694
MIR-154 0.000291465 0.013117986 0.007917705 0.007825175
MIR-155 0.016089386 0.043714602 0.05183188 0.030752846
MIR-181a,c 0.059130368 0.022451125 0.030012848 0.031324108
MIR-181b-1,2 0.026068865 0.049510131 0.054644232 0.028201471
MIR-182 1.630877102 1.254717928 0.5227306 1.240326739
MIR-183 0.038337806 0.027194356 0.013066885 0.052034588
MIR-184 0.094840006 0.534222842 0.791623047 0.432025454
MIR-185 0.478874185 1.151945186 1.153850266 0.84028534
MIR-186-P 0.684479894 0.693917026 1.265761875 0.756385556
MIR-187 0.003844267 0.021046885 0.073001024 0.037118423
MIR-188b-P 0.005062757 0.012186588 0.032858946 0.008696932
MIR-190 0.002807551 0.000237945 0.000601121
MIR-191 0.001163652 0.001602566 0.017670627 0.007974997
MIR-192 0.020216605 0.079064593 0.176425318 0.090882524
MIR-193 0.002948186 0.016055635 0.05243682 0.039464963
MIR-194-1,2 0.005533913 0.011547102 0.044280659 0.010839423
MIR-195-P 0.350408474 0.96448892 2.672581641 1.324131331
MIR-196a-1 0.01698214 0.093533668 0.191996514 0.091833365
MIR-196a-2 0.052658695 0.039967775 0.067355241 0.061740361
MIR-196b 3.736652932 0.49512547 0.492338348 0.34051806
MIR-197 1.369599015 2.514929694 8.535493209 3.578360453
MIR-198 0.001236303 0.101539965 0.065339717 0.044099381
MIR-199a-1-P 0.495837252 1.450978929 1.982502272 2.221685677
MIR-199a-2-P 0.020536556 0.070677341 0.127759327 0.102305827
MIR-199b-P 0.177735783 0.603460426 1.658551166 0.742590734
MIR-200a-P 8.22191564 21.15355259 32.34254987 22.02274227
MIR-200b 0.016848857 0.031719956 0.076784 0.049138384
MIR-200c 0.002160144 0.034314248 0.013605031 0.011832459
MIR-203 0.004350579 0.001443964 0.001990637 0.005267192
MIR-204 0.11033528 0.270174153 0.829592939 0.385331683
MIR-205 0.000381588 0.036009992 0.020365182 0.046785968
MIR-206 0.005075737 0.017904513 0.083588572 0.054113926
MIR-208 0.008016233 0.011840986 0.047682147 0.027384014
MIR-210 0.311220192 2.148108804 8.184220526 2.770512199
MIR-211 0.349769663 0.999916181 2.04359742 1.739338488
MIR-212 0.08178038 0.069869667 0.325163453 0.180127795
MIR-213 0.055734601 0.041444388 0.065005834 0.036332784
MIR-214 0.202760997 0.338462304 0.946441027 0.476409379
MIR-215 0.054823778 0.166206657 0.048953235 0.071482937
MIR-216 0.019265428 0.080884079 0.118579253 0.049079359
MIR-217 0.000418496 0.001202333 0.009433799 0.001456757
MIR-218-1,2 0.001495712 0.009202711 0.032168735 0.01780271
MIR-219-1,2 0.011332328 0.006684591 0.030460333 0.011190317
MIR-220 0.021096801 0.029541401 0.139721508 0.066215979
Continued
196
Table A. continued
MIR-221 0.110905865 0.121730329 0.142014291 0.13505334
MIR-222-P 0.112327383 0.190940238 0.132093009 0.063909279
MIR-223 0.007593202 0.007904509 0.021832309 0.014428731
MIR-224 0.157707652 0.106133615 0.118615465 0.095752451
MIR-296 0.008407765 0.030691832 0.098439287 0.056547017
MIR-299-P 0.078299292 0.115255106 0.282519043 0.212813521
MIR-301 0.021283527 0.090568462 0.149821442 0.132081354
MIR-302 0.018505778 0.021012201 0.036365419 0.022461954
MIR-302b 0.017382544 0.078936566 0.117106618 0.049917802
MIR-302c 0.02333714 0.024370558 0.051116311 0.034354686
MIR-302d 0.002463914 0.025930396 0.074527156 0.022004856
MIR-320 0.049871557 0.060541325 0.147684622 0.060940292
MIR-321 2.041175272 1.289958426 1.619070177 1.32337343
MIR-323 0.031151632 0.047227438 0.069135404 0.031733198
MIR-324-P 0.07795982 0.114396123 0.238114523 0.064816221
MIR-325 0.007246477 0.021813016 0.024580814 0.012525133
MIR-326-P 0.004937004 0.005051491 0.012497042 0.008518684
MIR-328 0.142401581 0.389305483 1.208252952 0.546026441
MIR-330 0.024454234 0.032544354 0.138415462 0.08220436
MIR-331-P 0.047200406 0.147267764 0.176748395 0.14511567
MIR-335 0.001089579 0.028130384 0.007656915 0.00838748
MIR-337 0.02737863 0.094483163 0.236712618 0.132575879
MIR-338 0.035246702 0.09626089 0.338815086 0.124527538
MIR-339 0.128454209 0.196915567 0.393681963 0.187664441
MIR-340 0.127528276 0.091999834 0.186578612 0.128098432
MIR-342 0.03043408 0.080770908 0.216630611 0.104742672
MIR-345 0.127470743 0.537801457 1.048298457 0.710367124
MIR-346 1.213669467 2.036278752 2.016020095 1.710536891
MIR-361 0.039135917 0.066620157 0.063305735 0.045357399
MIR-367 0.018859341 0.026131433 0.009801244 0.00997665
MIR-368 0.001189537
MIR-369 0.000283338
MIR-370 0.003686426 0.004071542 0.068177264 0.023037879
MIR-371 0.067913023 0.214636088 0.631794603 0.313783244
MIR-372 0.000994048 0.000750959 0.003778768 0.002828137
MIR-373 0.010972002 0.039190616 0.13619664 0.051928909
MIR-374-P 0.010169867 0.01010349 0.004821966
MIR-375 0.001827666 0.007745586 0.022122688 0.009557382
MIR-376 0.000294884 0.001095805 0.004766586 0.002611058
MIR-377 0.020755595 0.032653344 0.112177327 0.051069959
MIR-378 0.030074469 0.028584088 0.104060108 0.043706699
MIR-379 0.000952635 0.001254 0.005160251
MIR-380 0.000294495 0.00097759 0.00379323 0.000723768
MIR-381 0.000647896 0.003422718 0.012407429 0.00666265
MIR-382 0.004980409 0.003448936 0.046626114 0.006732531
MIR-383 0.004711872 0.011586536 0.053446712 0.026420603
Continued
197
Table A. continued
MIR-384 0.000249289 0.000548724 0.000212704
MIR-422b 0.057932835 0.134893204 0.242676955 0.211581768
MIR-423 0.01027527 0.022001651 0.06636151 0.036148954
MIR-424 0.00931013 0.002139775 0.008987201 0.005215917
MIR-425 0.013000519 0.009358127 0.034758895 0.04344016
18S rRNA (CT) 7.5105845 8.1212795 7.68454695 7.41360115
198
Figure A. Heatmap of miRNA precursor expression in pancreatic samples.
A. The relative expression of 201 miRNA precursors was determined by real-time PCR;
data are presented as ∆CT. Unsupervised hierarchical clustering was performed on a
subset of the entire data set; data are unfiltered. A median expression value equal to one
was designated black; red increased expression; green, reduced expression; grey,
undetectable expression. B. Dendrogram representing the results of hierarchical
clustering analysis of the miRNA precursor expression pattern in 47 samples. Sample
include primary pancreatic tumors (yellow, N = 28), normal pancreatic tissues (blue, N =
6), chronic pancreatitis (orange, N = 4) and pancreatic cancer cell lines (turquoise, N = 9).
199
APPENDIX B
DATA RELEVANT TO CHAPTER 3
200
miRNAs PANC-1 Miapaca-2 PL45 Mpanc-96
hsa-let-7a 864.050 610.148 692.112 876.530
hsa-let-7b 1474.656 1963.588 2611.949 2520.782
hsa-let-7c 44.810 68.821 97.517 114.943
hsa-let-7d 5.421 93.119 43.893 85.019
hsa-let-7e 24.693 28.415 47.948 32.652
hsa-let-7f 0.000 0.000 0.000 0.000
hsa-let-7g 420.650 442.835 316.674 1081.594
hsa-let-7i 118.281 393.891 63.906 560.419
hsa-miR-001 213.734 0.000 0.000 0.000
hsa-miR-007 79.325 231.578 258.703 220.865
hsa-miR-010a 3098.537 526.233 710.944 3555.518
hsa-miR-010b 208.210 2.341 7.859 155.023
hsa-miR-015a 136.041 194.730 118.999 175.215
hsa-miR-015b 5759.848 5761.402 5575.903 3631.885
hsa-miR-016 3475.243 3487.545 3978.712 3726.583
hsa-miR-017-3p 8.642 13.903 10.035 37.234
hsa-miR-017-5p 766.778 746.268 402.184 695.511
hsa-miR-018 65.312 124.656 25.865 78.355
hsa-miR-018b 0.000 0.000 0.000 0.000
hsa-miR-019a 977.282 2804.102 930.467 2128.957
hsa-miR-019b 5694.475 9111.486 5122.005 11294.600
hsa-miR-020 7364.867 10422.994 5679.185 12659.767
hsa-miR-020b 29.127 32.889 54.914 39.529
hsa-miR-021 2830.742 2438.259 2273.815 4791.171
hsa-miR-022 72.957 81.611 23.991 45.179
hsa-miR-023a 47.623 60.197 84.017 55.265
hsa-miR-023b 86.425 30.806 23.786 310.926
hsa-miR-023bN 2158.286 277.655 590.804 4914.756
hsa-miR-024 15456.092 15628.700 11551.171 23228.328
hsa-miR-025 712.021 739.761 659.609 934.655
hsa-miR-026aN 2092.323 944.888 1946.820 5468.540
hsa-miR-026b 1285.701 864.814 1327.705 3368.339
hsa-miR-026bN 573.340 175.721 0.000 1568.819
hsa-miR-027a 1480.905 2334.120 1474.833 1526.871
hsa-miR-027b 209.614 37.791 72.515 733.133
hsa-miR-027bN 1917.231 336.787 597.086 6020.465
hsa-miR-028 486.117 299.220 302.150 499.902
hsa-miR-029a 1835.877 2770.324 2476.119 3207.799
Continued
Table B. mature miRNA expression in pancreas cell lines.
Data are presented as copies of miRNA per cell.
201
Table B. continued
hsa-miR-029b 30.828 117.666 37.678 49.756
hsa-miR-029c 115.697 143.373 157.860 228.731
hsa-miR-030a-3p 2180.979 1887.253 1471.714 1025.028
hsa-miR-030c 6212.199 3016.941 3929.825 4837.394
hsa-miR-030d 510.943 132.479 442.222 940.854
hsa-miR-030e-3p 346.787 137.825 103.274 207.163
hsa-miR-031 14804.605 7.356 26384.031 14512.570
hsa-miR-032 49.803 125.343 33.579 41.937
hsa-miR-033 1.396 10.720 3.637 0.000
hsa-miR-034a 12.535 0.000 0.000 1.043
hsa-miR-034aN 49.334 0.000 14.296 0.000
hsa-miR-034b 1.687 7.955 0.000 0.000
hsa-miR-092N 6603.685 6110.632 4414.971 8329.002
hsa-miR-093 6586.754 9209.706 5769.697 7367.281
hsa-miR-095 8.873 23.383 31.929 28.131
hsa-miR-096 6653.265 3957.866 7663.497 4879.448
hsa-miR-098 85.958 107.388 110.607 85.914
hsa-miR-099a 5335.978 3.656 975.095 1261.087
hsa-miR-099b 403.800 358.360 380.070 298.054
hsa-miR-100 6756.446 0.000 1057.410 1313.591
hsa-miR-101 2.796 10.780 8.918 8.063
hsa-miR-103 492.989 433.180 284.603 513.420
hsa-miR-105 369.745 186.663 110.401 244.717
hsa-miR-106a 1123.657 510.667 707.918 912.946
hsa-miR-106b 890.556 1546.384 570.629 1003.441
hsa-miR-107 0.000 2.155 0.000 1.869
hsa-miR-108 0.000 0.000 0.000 0.000
hsa-miR-122a 0.000 0.000 0.000 0.000
hsa-miR-124a 0.000 0.000 0.000 0.000
hsa-miR-125a 2895.833 1984.226 3235.627 2885.609
hsa-miR-125b 2495.930 0.000 740.942 858.086
hsa-miR-126 827.387 1208.406 256.223 1151.908
hsa-miR-126* 113.682 160.048 45.041 159.173
hsa-miR-127 1952.821 0.000 0.000 0.000
hsa-miR-128a 0.000 0.000 0.000 0.000
hsa-miR-130a 246.117 301.649 97.281 3.154
hsa-miR-130aN 576.053 815.060 200.421 6.888
hsa-miR-130b 24.032 6.581 2.693 23.622
hsa-miR-132 536.345 352.690 108.404 226.632
hsa-miR-133a 51.221 0.000 4.624 0.000
hsa-miR-134 328.861 0.000 0.000 0.000
Continued
202
Table B. continued
hsa-miR-135a 9.756 18.647 15.274 21.882
hsa-miR-135b 83.673 402.734 540.723 499.139
hsa-miR-139 0.000 0.000 2.053 0.000
hsa-miR-140 419.579 1107.229 136.013 616.546
hsa-miR-141 5.253 2.603 318.910 121.750
hsa-miR-142-3p 0.000 0.000 0.000 0.000
hsa-miR-142-5p 4.454 2.571 2.202 8.790
hsa-miR-143 0.000 0.000 0.000 0.000
hsa-miR-145 1.080 0.000 8.260 6.709
hsa-miR-146 2022.389 241.074 261.745 1268.557
hsa-miR-146b 225.030 22.697 104.692 66.056
hsa-miR-147 0.000 0.000 0.000 0.000
hsa-miR-148a 7.923 19.597 16.990 0.000
hsa-miR-148b 109.169 60.215 31.270 91.221
hsa-miR-149 467.903 425.551 237.590 389.086
hsa-miR-150 5.302 10.623 11.357 418.904
hsa-miR-152 307.416 228.562 121.006 203.444
hsa-miR-152N 282.147 190.061 66.664 110.735
hsa-miR-153 0.000 0.000 0.000 0.000
hsa-miR-154 0.000 0.000 0.000 0.000
hsa-miR-154* 0.000 0.000 0.000 0.000
hsa-miR-155 509.124 10.975 223.197 322.275
hsa-miR-181a 99.637 0.000 59.778 505.728
hsa-miR-181b 713.205 105.385 415.634 2092.094
hsa-miR-181c 12.595 2.880 9.466 33.485
hsa-miR-181d 266.629 45.062 197.154 602.723
hsa-miR-182* 7.227 5.143 8.097 2.959
hsa-miR-183 245.306 401.057 150.450 243.460
hsa-miR-184 15.522 0.000 0.000 0.000
hsa-miR-186 1328.437 2129.946 618.104 1293.600
hsa-miR-187 135.515 11.274 0.000 0.000
hsa-miR-188 383.579 521.567 629.668 397.911
hsa-miR-188b 18.017 15.545 16.555 31.289
hsa-miR-190 0.000 0.000 0.000 0.000
hsa-miR-191 6749.922 2755.005 1256.139 3624.652
hsa-miR-192 107.284 190.029 72.533 14804.427
hsa-miR-193 200.340 130.367 54.820 54.030
hsa-miR-193b 2.319 0.000 1.831 0.000
hsa-miR-194 56.171 42.884 29.659 4032.189
hsa-miR-195 260.372 172.332 165.696 190.578
hsa-miR-196a 2020.987 24.667 12.565 1709.624
Continued
203
Table B. continued
hsa-miR-196b 0.000 0.000 0.000 140.840
hsa-miR-197 822.094 825.354 367.643 501.897
hsa-miR-199a 0.000 0.000 0.000 0.000
hsa-miR-199a* 0.000 0.000 3.487 0.000
hsa-miR-199b 0.000 0.000 0.000 0.000
hsa-miR-199-s 0.000 0.000 0.000 0.000
hsa-miR-200a 6.694 0.000 470.674 1001.577
hsa-miR-200a* 0.000 0.000 1.408 14.060
hsa-miR-200b 15.266 8.503 1433.556 2756.762
hsa-miR-200bN 7.323 3.151 651.476 1247.651
hsa-miR-200c 36.863 32.998 11833.947 4445.524
hsa-miR-200cN 26.774 10.587 12003.373 4263.624
hsa-miR-202 0.000 0.000 0.000 0.000
hsa-miR-202* 0.000 0.000 0.000 0.000
hsa-miR-203 9.644 0.000 422.577 16.102
hsa-miR-204 0.000 0.000 0.000 4.077
hsa-miR-205 0.000 0.000 233.410 0.000
hsa-miR-206 4.067 8.670 0.000 0.000
hsa-miR-208 0.000 0.000 0.000 0.000
hsa-miR-210 191.115 109.303 84.243 393.737
hsa-miR-210N 408.362 173.477 201.879 818.525
hsa-miR-211 0.000 1.738 0.000 0.000
hsa-miR-212 147.366 84.625 80.253 79.186
hsa-miR-213 31.440 0.000 10.486 111.251
hsa-miR-214 9.084 0.000 11.213 6.801
hsa-miR-215 14.019 0.000 0.000 55.928
hsa-miR-216 0.000 0.000 0.000 0.000
hsa-miR-217 0.000 1.014 4.797 0.000
hsa-miR-218 40.258 7.585 9.284 0.000
hsa-miR-219 0.000 0.000 0.000 0.000
hsa-miR-220 0.000 0.000 0.000 0.000
hsa-miR-221 1684.751 5026.917 2247.497 1510.636
hsa-miR-222 10090.208 26850.543 14378.551 15557.420
hsa-miR-223 5.059 6.392 0.000 12.204
hsa-miR-296 0.000 9.428 3.075 3.386
hsa-miR-299 0.000 0.000 0.000 0.000
hsa-miR-299-3p 0.000 0.000 0.000 0.000
hsa-miR-301 289.144 530.584 138.497 245.915
hsa-miR-302a 0.000 0.000 0.000 0.000
hsa-miR-302a* 0.000 0.000 0.000 0.000
hsa-miR-302b 11.686 6.143 14.718 2.928
Continued
204
Table B. continued
hsa-miR-302c 0.000 0.000 0.000 14.714
hsa-miR-302c* 0.000 0.000 0.000 0.000
hsa-miR-302d 0.000 0.000 0.000 0.000
hsa-miR-320 639.095 2664.822 531.259 2487.994
hsa-miR-321 7560.518 3685.384 3385.922 6463.011
hsa-miR-323 30.810 0.000 0.000 0.000
hsa-miR-324-3p 578.447 587.352 150.425 426.858
hsa-miR-324-5p 213.037 309.322 34.634 177.638
hsa-miR-325 1.707 1.227 2.309 1.656
hsa-miR-326 0.000 1.948 0.000 3.732
hsa-miR-328 81.036 157.912 57.546 191.665
hsa-miR-329 0.000 0.000 0.000 0.000
hsa-miR-330 9.699 17.584 4.314 11.096
hsa-miR-331 1741.754 1549.752 1247.694 1732.790
hsa-miR-335 154.016 0.000 74.827 33.366
hsa-miR-337 0.000 0.000 0.000 0.000
hsa-miR-338 0.000 11.127 9.954 0.000
hsa-miR-339 28.274 48.238 33.163 38.287
hsa-miR-340 93.409 87.527 42.445 65.627
hsa-miR-342 1074.575 0.000 0.000 12.481
hsa-miR-345 218.322 512.148 26.062 158.712
hsa-miR-346 5.678 0.000 5.614 7.678
hsa-miR-361 166.953 121.277 124.104 276.048
hsa-miR-362 8.808 15.993 10.860 13.851
hsa-miR-363 0.000 0.000 0.000 0.000
hsa-miR-365 876.765 355.292 346.851 478.339
hsa-miR-367 0.000 0.000 0.000 0.000
hsa-miR-368 0.000 0.000 0.000 0.000
hsa-miR-369 0.000 0.000 0.000 0.000
hsa-miR-369-5p 23.844 0.000 0.000 0.000
hsa-miR-370 480.461 42.935 27.846 54.579
hsa-miR-371 0.000 0.000 0.000 0.000
hsa-miR-372 0.000 0.000 0.000 0.000
hsa-miR-373 0.000 0.000 0.000 0.000
hsa-miR-374 1048.932 1208.292 381.134 1904.360
hsa-miR-375 10.015 28.024 297.572 14.582
hsa-miR-376a 454.336 0.000 0.000 5.407
hsa-miR-376b 0.000 0.000 0.000 0.000
hsa-miR-377 4.017 0.000 0.000 0.000
hsa-miR-378 66.322 82.101 17.322 42.386
hsa-miR-379 87.029 0.000 0.000 7.725
Continued
205
Table B. continued
hsa-miR-380-3p 0.000 0.000 0.000 0.000
hsa-miR-380-5p 13.915 0.000 0.000 0.000
hsa-miR-381 0.000 0.000 0.000 0.000
hsa-miR-382 657.786 0.000 0.000 4.158
hsa-miR-383 0.000 0.000 2.547 0.000
hsa-miR-384 0.000 0.000 0.000 0.000
hsa-miR-409-5p 3.894 0.000 0.000 0.000
hsa-miR-410 0.000 0.000 0.000 0.000
hsa-miR-412 0.000 0.000 0.000 0.000
hsa-miR-422b 57.130 25.544 14.357 20.773
hsa-miR-423 434.353 300.457 231.107 242.732
hsa-miR-425 286.101 96.894 23.421 68.294
hsa-miR-429 0.000 0.000 71.396 126.156
hsa-miR-431 211.319 319.630 348.651 275.681
hsa-miR-432 2.803 0.000 0.000 0.000
hsa-miR-432* 0.000 0.000 0.000 0.000
hsa-miR-433 94.334 0.000 0.000 0.000
hsa-miR-448 0.000 0.000 0.000 0.000
hsa-miR-449 7.233 0.000 9.279 6.456
hsa-miR-450 0.000 0.000 0.000 0.000
hsa-miR-451 0.000 0.000 0.000 0.000
hsa-miR-452 0.000 0.000 0.000 3.134
hsa-miR-452* 5.756 0.000 13.718 131.110
hsa-miR-453 0.000 0.000 0.000 0.000
hsa-miR-485-3p 14.821 0.000 0.000 0.000
hsa-miR-485-5p 0.000 0.000 0.000 0.000
hsa-miR-488 0.000 0.000 3.197 0.000
hsa-miR-489 0.000 4.417 0.000 22.000
hsa-miR-490 0.000 0.000 2.628 1.634
hsa-miR-491 32.483 0.000 49.550 15.174
hsa-miR-492 1.194 0.000 2.088 1.031
hsa-miR-493 0.000 0.000 0.000 0.000
hsa-miR-494 0.000 0.000 0.000 0.000
hsa-miR-495 2.887 0.000 0.000 0.000
hsa-miR-496 2.759 3.250 1.830 2.165
hsa-miR-497 2.028 0.000 0.000 0.000
hsa-miR-498 0.000 0.000 0.000 0.000
hsa-miR-499 0.000 0.000 0.000 0.000
hsa-miR-500 1.027 9.896 0.000 0.000
hsa-miR-501 35.130 82.369 1.463 1.410
hsa-miR-502 32.843 30.164 6.356 1.690
Continued
206
Table B. continued
hsa-miR-503 3.298 0.000 1.836 0.000
hsa-miR-504 0.000 0.000 0.000 0.000
hsa-miR-505 0.000 0.000 0.000 0.000
hsa-miR-506 0.000 0.000 0.000 0.000
hsa-miR-507 0.000 0.000 0.000 0.000
hsa-miR-508 0.000 0.000 0.000 0.000
hsa-miR-509 0.000 0.000 0.000 0.000
hsa-miR-510 0.000 0.000 0.000 0.000
hsa-miR-511 2.567 0.000 0.000 1.062
hsa-miR-512-3p 1.717 1.805 2.120 1.647
hsa-miR-512-5p 0.000 0.000 0.000 0.000
hsa-miR-513 0.000 0.000 0.000 0.000
hsa-miR-514 0.000 0.000 0.000 0.000
hsa-miR-515-3p 0.000 0.000 0.000 0.000
hsa-miR-515-5p 0.000 0.000 0.000 0.000
hsa-miR-516-3p 960.116 1168.878 863.947 1079.079
hsa-miR-516-5p 0.000 0.000 0.000 0.000
hsa-miR-517* 0.000 0.000 0.000 0.000
hsa-miR-517a 0.000 0.000 0.000 0.000
hsa-miR-517b 0.000 0.000 0.000 0.000
hsa-miR-517c 3.523 0.000 0.000 0.000
hsa-miR-518a 0.000 0.000 0.000 0.000
hsa-miR-518a-2* 0.000 0.000 0.000 0.000
hsa-miR-518b 8.894 7.300 1.703 9.942
hsa-miR-518c 0.000 0.000 0.000 0.000
hsa-miR-518c* 0.000 0.000 0.000 0.000
hsa-miR-518d 0.000 0.000 0.000 0.000
hsa-miR-518e 0.000 0.000 0.000 0.000
hsa-miR-518f 9.541 10.827 8.377 10.899
hsa-miR-518f* 0.000 0.000 0.000 0.000
hsa-miR-519a 0.000 0.000 0.000 0.000
hsa-miR-519b 0.000 0.000 0.000 0.000
hsa-miR-519c 0.000 0.000 0.000 0.000
hsa-miR-519d 0.000 0.000 0.000 0.000
hsa-miR-519e 0.000 0.000 0.000 0.000
hsa-miR-519e* 0.000 0.000 0.000 0.000
hsa-miR-520a 0.000 0.000 0.000 0.000
hsa-miR-520a* 0.000 0.000 0.000 0.000
hsa-miR-520b 0.000 0.000 0.000 0.000
hsa-miR-520c 0.000 0.000 0.000 0.000
hsa-miR-520d 0.000 0.000 0.000 0.000
Continued
207
Table B. continued
hsa-miR-520d* 0.000 0.000 0.000 0.000
hsa-miR-520e 0.000 0.000 0.000 0.000
hsa-miR-520f 0.000 0.000 0.000 0.000
hsa-miR-520g 0.000 0.000 0.000 0.000
hsa-miR-520h 0.000 0.000 0.000 0.000
hsa-miR-521 0.000 0.000 0.000 0.000
hsa-miR-522 0.000 0.000 0.000 0.000
hsa-miR-523 0.000 0.000 0.000 0.000
hsa-miR-524 0.000 3.378 0.000 0.000
hsa-miR-524* 0.000 0.000 0.000 0.000
hsa-miR-525 0.000 0.000 0.000 0.000
hsa-miR-525* 0.000 0.000 0.000 0.000
hsa-miR-526a 5.233 0.000 0.000 3.382
hsa-miR-526b 0.000 0.000 0.000 0.000
hsa-miR-526b* 0.000 0.000 0.000 0.000
hsa-miR-526c 0.000 0.000 0.000 0.000
hsa-miR-527 0.000 0.000 0.000 0.000
mmu-miR-129-2 0.000 0.000 0.000 0.000
mmu-miR-137 9.284 90.286 0.000 0.000
mmu-miR-224 0.000 0.000 0.000 161.951
mmu-miR-424 0.000 0.000 0.000 0.000
miRNAs HS766T CaPan-1 SW1990 HPNE
hsa-let-7a 1230.081 1343.353 1263.019 605.962
hsa-let-7b 1870.761 6985.835 8055.159 4187.560
hsa-let-7c 107.477 157.190 297.774 114.545
hsa-let-7d 78.436 247.065 133.216 28.820
hsa-let-7e 45.127 35.538 88.329 30.823
hsa-let-7f 0.000 121.826 71.422 5.908
hsa-let-7g 1161.567 3031.906 873.430 282.382
hsa-let-7i 493.237 544.535 420.083 80.513
hsa-miR-001 0.000 15.051 0.000 0.000
hsa-miR-007 1012.601 937.597 48.221 14.883
hsa-miR-010a 347.279 1954.989 714.767 1394.986
hsa-miR-010b 35.477 86.905 21.067 42.125
hsa-miR-015a 332.178 301.383 296.721 80.922
hsa-miR-015b 4744.868 7517.884 4741.230 1200.995
hsa-miR-016 4131.638 23216.771 14016.092 2512.889
hsa-miR-017-3p 25.878 55.282 21.189 9.003
hsa-miR-017-5p 1380.350 752.711 1293.216 315.911
hsa-miR-018 364.269 350.333 234.236 50.776
Continued
208
Table B. continued
hsa-miR-018b 0.000 0.000 0.000 0.000
hsa-miR-019a 6318.760 1495.374 1293.129 529.616
hsa-miR-019b 23081.349 8009.110 12500.154 3190.566
hsa-miR-020 26691.162 11682.849 17932.811 2490.852
hsa-miR-020b 106.782 111.376 23.815 6.805
hsa-miR-021 8821.574 15409.072 4669.247 2087.704
hsa-miR-022 68.976 319.141 320.670 428.544
hsa-miR-023a 57.620 118.519 165.938 69.734
hsa-miR-023b 58.732 333.215 242.951 30.653
hsa-miR-023bN 537.732 2602.756 4106.748 2134.444
hsa-miR-024 12423.694 24900.704 30833.748 14084.569
hsa-miR-025 1548.002 1750.739 532.804 261.889
hsa-miR-026aN 3038.350 4458.238 2478.393 1521.919
hsa-miR-026b 2099.444 1947.564 1826.644 443.561
hsa-miR-026bN 1025.426 1313.722 1043.522 286.698
hsa-miR-027a 1768.429 3478.949 6465.401 3559.906
hsa-miR-027b 97.459 783.443 528.889 241.408
hsa-miR-027bN 2008.269 4809.437 2516.272 454.336
hsa-miR-028 559.050 1324.563 523.289 361.597
hsa-miR-029a 8124.016 3084.855 5753.919 3515.821
hsa-miR-029b 369.113 86.659 262.013 40.482
hsa-miR-029c 644.829 455.700 302.248 219.232
hsa-miR-030a-3p 19.117 1575.964 5670.309 536.095
hsa-miR-030c 2155.741 9266.575 17675.582 1176.811
hsa-miR-030d 823.568 1815.420 615.465 91.388
hsa-miR-030e-3p 156.083 414.980 604.461 144.368
hsa-miR-031 19942.068 0.000 31129.427 2122.582
hsa-miR-032 194.211 81.655 47.929 23.753
hsa-miR-033 0.000 15.118 0.000 0.000
hsa-miR-034a 2.347 81.504 171.386 65.499
hsa-miR-034aN 108.398 512.232 1058.130 577.213
hsa-miR-034b 0.000 19.256 19.865 12.382
hsa-miR-092N 12125.994 10970.557 18541.166 2862.333
hsa-miR-093 16351.386 18053.346 7062.033 1352.865
hsa-miR-095 33.619 88.552 6.537 0.000
hsa-miR-096 12850.829 23034.217 4325.126 31648.318
hsa-miR-098 258.059 150.612 125.405 19.961
hsa-miR-099a 4416.500 167.790 3575.349 8310.965
hsa-miR-099b 587.805 280.100 567.198 356.239
hsa-miR-100 4187.824 228.749 4369.825 12384.337
hsa-miR-101 41.897 41.807 36.456 5.212
Continued
209
Table B. continued
hsa-miR-103 1125.711 1005.124 731.269 184.123
hsa-miR-105 330.355 0.000 0.000 0.000
hsa-miR-106a 2434.386 2082.088 1167.924 2363.999
hsa-miR-106b 3647.757 3304.220 1315.166 250.698
hsa-miR-107 1.959 15.750 31.695 0.000
hsa-miR-108 0.000 0.000 0.000 0.000
hsa-miR-122a 0.000 0.000 2.636 0.000
hsa-miR-124a 0.000 0.000 0.000 0.000
hsa-miR-125a 3827.792 3359.685 9669.414 5131.341
hsa-miR-125b 1255.494 111.703 1958.705 13036.950
hsa-miR-126 3848.807 577.380 441.293 54.490
hsa-miR-126* 630.679 93.165 69.148 18.806
hsa-miR-127 0.000 24.931 42.675 2273.944
hsa-miR-128a 0.000 0.000 0.000 0.000
hsa-miR-130a 9.510 180.776 1111.568 221.231
hsa-miR-130aN 0.000 427.174 1795.276 397.051
hsa-miR-130b 78.839 355.509 451.201 164.759
hsa-miR-132 462.927 507.215 506.850 581.839
hsa-miR-133a 2.454 4.241 0.000 0.000
hsa-miR-134 2.964 681.593 568.509 1222.877
hsa-miR-135a 11.286 40.137 35.974 0.000
hsa-miR-135b 292.133 1194.599 1716.795 0.000
hsa-miR-139 0.000 0.000 1.739 0.000
hsa-miR-140 1617.380 272.987 554.315 1288.762
hsa-miR-141 9.082 1506.712 1204.831 0.000
hsa-miR-142-3p 5.654 11.362 5.569 0.000
hsa-miR-142-5p 4.260 10.001 3.795 10.382
hsa-miR-143 0.000 0.000 0.000 20.678
hsa-miR-145 5.650 3.945 6.095 302.601
hsa-miR-146 322.064 7358.944 373.788 88.525
hsa-miR-146b 65.501 456.306 130.435 34.729
hsa-miR-147 0.000 0.000 0.000 0.000
hsa-miR-148a 234.763 1630.279 308.654 0.000
hsa-miR-148b 148.676 372.873 223.158 50.074
hsa-miR-149 93.256 283.171 1061.771 212.812
hsa-miR-150 10.961 18.824 169.116 15.944
hsa-miR-152 628.287 523.679 465.356 1032.125
hsa-miR-152N 599.658 692.663 556.732 1135.140
hsa-miR-153 0.000 7.434 0.000 0.000
hsa-miR-154 0.000 0.000 0.000 0.000
hsa-miR-154* 0.000 0.000 0.000 8.270
Continued
210
Table B. continued
hsa-miR-155 2137.202 188.687 583.550 294.940
hsa-miR-181a 17.400 964.439 904.511 356.895
hsa-miR-181b 266.273 1637.691 2029.040 393.906
hsa-miR-181c 41.747 39.454 47.768 9.065
hsa-miR-181d 362.719 741.119 1479.025 383.596
hsa-miR-182* 24.720 13.045 7.770 2.086
hsa-miR-183 565.677 198.625 266.500 20.418
hsa-miR-184 0.000 27.011 15.719 13.788
hsa-miR-186 2176.914 3285.703 1386.103 1415.933
hsa-miR-187 0.000 0.000 0.000 0.000
hsa-miR-188 559.796 379.661 0.000 0.000
hsa-miR-188b 23.751 28.448 33.452 34.604
hsa-miR-190 0.000 25.343 1.004 1.287
hsa-miR-191 4498.375 7857.945 2541.623 1219.360
hsa-miR-192 285.059 20713.292 92.498 10.457
hsa-miR-193 47.319 79.671 338.336 16.534
hsa-miR-193b 0.000 1.705 0.000 2.857
hsa-miR-194 78.359 8254.343 41.730 4.993
hsa-miR-195 175.615 310.565 115.463 36.794
hsa-miR-196a 448.196 433.017 217.178 0.000
hsa-miR-196b 15.217 1.432 0.000 0.000
hsa-miR-197 579.670 683.293 473.212 409.397
hsa-miR-199a 0.000 0.000 0.000 4.078
hsa-miR-199a* 0.000 3.552 6.243 615.044
hsa-miR-199b 0.000 0.000 0.000 2.950
hsa-miR-199-s 0.000 0.000 0.000 95.152
hsa-miR-200a 1753.483 10033.353 1240.690 7.685
hsa-miR-200a* 8.560 35.373 0.000 2.569
hsa-miR-200b 1989.668 13394.016 1726.807 0.000
hsa-miR-200bN 1193.297 11574.351 1117.805 0.000
hsa-miR-200c 157.598 25566.636 27898.251 5.807
hsa-miR-200cN 13.356 21426.505 18828.799 11.024
hsa-miR-202 0.000 0.000 1.590 2.351
hsa-miR-202* 0.000 0.000 0.000 0.000
hsa-miR-203 2160.283 6149.604 2850.220 0.000
hsa-miR-204 0.000 0.000 0.000 1073.849
hsa-miR-205 0.000 12.517 1812.285 0.000
hsa-miR-206 2.954 0.000 4.148 0.000
hsa-miR-208 0.000 0.000 0.000 0.000
hsa-miR-210 102.257 806.164 428.513 174.681
hsa-miR-210N 263.965 2223.338 882.036 467.532
Continued
211
Table B. continued
hsa-miR-211 0.000 0.000 0.000 0.000
hsa-miR-212 126.575 78.580 144.966 112.896
hsa-miR-213 10.821 145.462 185.144 66.503
hsa-miR-214 2.098 3.894 1.564 2330.115
hsa-miR-215 6.570 44.638 0.000 2.848
hsa-miR-216 0.000 0.000 0.000 0.000
hsa-miR-217 0.000 0.000 0.000 1.520
hsa-miR-218 1.300 0.000 371.070 168.110
hsa-miR-219 0.000 0.000 0.000 28.468
hsa-miR-220 0.000 0.000 0.000 0.000
hsa-miR-221 4584.006 2795.365 11340.689 6383.703
hsa-miR-222 30537.231 14207.475 50548.416 30388.349
hsa-miR-223 1.246 12.086 6.564 7.594
hsa-miR-296 6.712 922.706 4.709 43.272
hsa-miR-299 0.000 0.000 0.000 0.000
hsa-miR-299-3p 0.000 0.000 66.344 99.348
hsa-miR-301 983.825 199.935 326.238 40.694
hsa-miR-302a 0.000 0.000 0.000 33.565
hsa-miR-302a* 0.000 0.000 0.000 0.000
hsa-miR-302b 8.425 69.915 145.936 173.138
hsa-miR-302c 0.000 0.000 0.000 31.408
hsa-miR-302c* 0.000 0.000 0.000 0.000
hsa-miR-302d 0.000 0.000 1.529 55.058
hsa-miR-320 1161.400 1832.677 2212.398 546.854
hsa-miR-321 4799.042 16652.105 9201.774 4799.142
hsa-miR-323 0.000 0.000 0.000 11.742
hsa-miR-324-3p 1235.105 369.688 422.612 131.295
hsa-miR-324-5p 1036.678 541.087 271.914 107.849
hsa-miR-325 2.574 0.000 0.000 0.000
hsa-miR-326 8.178 1.137 0.000 0.000
hsa-miR-328 224.212 192.866 377.848 102.847
hsa-miR-329 0.000 0.000 0.000 0.000
hsa-miR-330 26.528 21.571 33.648 1.029
hsa-miR-331 1206.615 4792.239 1866.307 493.060
hsa-miR-335 114.012 139.660 321.407 11.130
hsa-miR-337 0.000 0.000 0.000 0.000
hsa-miR-338 0.000 41.650 0.000 0.000
hsa-miR-339 32.684 98.825 78.484 25.924
hsa-miR-340 116.539 100.997 75.191 34.890
hsa-miR-342 7.999 485.986 14.409 243.629
hsa-miR-345 863.672 420.297 490.352 84.162
Continued
212
Table B. continued
hsa-miR-346 10.275 27.980 24.326 0.000
hsa-miR-361 208.257 305.695 309.550 143.547
hsa-miR-362 9.367 3.768 25.012 10.654
hsa-miR-363 0.000 0.000 0.000 0.000
hsa-miR-365 168.404 419.028 26.289 14.586
hsa-miR-367 0.000 3.484 0.000 21.045
hsa-miR-368 0.000 0.000 0.000 0.000
hsa-miR-369 0.000 0.000 0.000 2.792
hsa-miR-369-5p 0.000 0.000 0.000 0.000
hsa-miR-370 19.938 103.808 126.559 530.437
hsa-miR-371 0.000 0.000 0.000 0.000
hsa-miR-372 0.000 258.181 227.498 104.621
hsa-miR-373 0.000 8.521 0.000 0.000
hsa-miR-374 1766.875 795.440 769.622 424.904
hsa-miR-375 9.792 7695.302 233.070 0.000
hsa-miR-376a 0.000 4.989 0.000 379.324
hsa-miR-376b 0.000 0.000 0.000 0.000
hsa-miR-377 0.000 0.000 0.000 4.113
hsa-miR-378 47.868 105.177 28.631 0.000
hsa-miR-379 0.000 7.566 1.621 89.641
hsa-miR-380-3p 0.000 0.000 0.000 0.000
hsa-miR-380-5p 0.000 0.000 7.624 8.699
hsa-miR-381 0.000 0.000 0.000 6.121
hsa-miR-382 0.000 9.162 0.000 254.060
hsa-miR-383 0.000 4.304 6.354 10.057
hsa-miR-384 0.000 0.000 0.000 0.000
hsa-miR-409-5p 0.000 0.000 0.000 0.000
hsa-miR-410 0.000 0.000 3.078 0.000
hsa-miR-412 0.000 0.000 0.000 0.000
hsa-miR-422b 14.104 57.014 28.176 3.285
hsa-miR-423 281.004 611.035 701.210 227.045
hsa-miR-425 80.644 187.599 91.016 20.357
hsa-miR-429 233.282 868.079 0.000 0.000
hsa-miR-431 325.854 102.400 0.000 3.097
hsa-miR-432 0.000 0.000 195.799 227.988
hsa-miR-432* 0.000 1.407 231.798 0.000
hsa-miR-433 0.000 0.000 179.318 46.546
hsa-miR-448 0.000 0.000 0.000 0.000
hsa-miR-449 53.301 7.283 0.000 0.000
hsa-miR-450 0.000 0.000 0.000 3.178
hsa-miR-451 0.000 0.000 0.000 0.000
Continued
213
Table B. continued
hsa-miR-452 0.000 0.000 0.000 8.297
hsa-miR-452* 0.000 52.765 1678.851 197.182
hsa-miR-453 0.000 0.000 0.000 0.000
hsa-miR-485-3p 0.000 0.000 9.359 16.103
hsa-miR-485-5p 0.000 0.000 0.000 0.000
hsa-miR-488 0.000 0.000 0.000 0.000
hsa-miR-489 0.000 4.757 41969.654 1448.000
hsa-miR-490 0.000 1.371 5975.722 3903.870
hsa-miR-491 156.672 0.000 0.000 0.000
hsa-miR-492 0.000 43.254 38.559 3.028
hsa-miR-493 0.000 0.000 0.000 0.000
hsa-miR-494 1.281 0.000 0.000 0.000
hsa-miR-495 0.000 0.000 8.217 0.000
hsa-miR-496 0.000 3.479 5.179 7.017
hsa-miR-497 0.000 1.963 0.000 0.000
hsa-miR-498 0.000 0.000 249.153 300.940
hsa-miR-499 0.000 0.000 1162.533 331.758
hsa-miR-500 0.000 0.000 0.000 0.000
hsa-miR-501 27.548 6.703 3.427 0.000
hsa-miR-502 7.486 4.154 7.851 0.000
hsa-miR-503 0.000 0.000 0.000 6.121
hsa-miR-504 0.000 0.000 0.000 0.000
hsa-miR-505 0.000 0.000 0.000 0.000
hsa-miR-506 0.000 0.000 0.000 0.000
hsa-miR-507 0.000 0.000 0.000 0.000
hsa-miR-508 0.000 0.000 0.000 0.000
hsa-miR-509 0.000 1.460 5.563 0.000
hsa-miR-510 0.000 0.000 0.000 0.000
hsa-miR-511 0.000 0.000 0.000 0.000
hsa-miR-512-3p 7.494 0.000 0.000 6.048
hsa-miR-512-5p 0.000 0.000 0.000 0.000
hsa-miR-513 0.000 0.000 0.000 0.000
hsa-miR-514 0.000 0.000 0.000 0.000
hsa-miR-515-3p 0.000 0.000 0.000 0.000
hsa-miR-515-5p 0.000 0.000 0.000 0.000
hsa-miR-516-3p 1082.658 152.779 55.446 527.907
hsa-miR-516-5p 0.000 0.000 0.000 0.000
hsa-miR-517* 0.000 0.000 0.000 0.000
hsa-miR-517a 0.000 0.000 0.000 0.000
hsa-miR-517b 0.000 0.000 0.000 0.000
hsa-miR-517c 1.670 0.000 0.000 0.000
Continued
214
Table B. continued
hsa-miR-518a 0.000 0.000 0.000 0.000
hsa-miR-518a-2* 0.000 0.000 0.000 0.000
hsa-miR-518b 12.890 6.953 4.007 5.351
hsa-miR-518c 0.000 0.000 0.000 0.000
hsa-miR-518c* 0.000 0.000 0.000 0.000
hsa-miR-518d 0.000 0.000 0.000 0.000
hsa-miR-518e 0.000 0.000 0.000 0.000
hsa-miR-518f 9.566 2.828 1.097 2.149
hsa-miR-518f* 0.000 0.000 0.000 0.000
hsa-miR-519a 0.000 0.000 0.000 0.000
hsa-miR-519b 0.000 0.000 0.000 0.000
hsa-miR-519c 0.000 0.000 0.000 0.000
hsa-miR-519d 1.560 0.000 0.000 0.000
hsa-miR-519e 0.000 1.458 0.000 0.000
hsa-miR-519e* 0.000 0.000 0.000 0.000
hsa-miR-520a 0.000 0.000 0.000 0.000
hsa-miR-520a* 0.000 0.000 0.000 0.000
hsa-miR-520b 0.000 0.000 0.000 0.000
hsa-miR-520c 0.000 0.000 0.000 0.000
hsa-miR-520d 0.000 0.000 0.000 0.000
hsa-miR-520d* 0.000 0.000 0.000 0.000
hsa-miR-520e 0.000 0.000 0.000 0.000
hsa-miR-520f 0.000 0.000 0.000 0.000
hsa-miR-520g 0.000 0.000 0.000 0.000
hsa-miR-520h 0.000 0.000 0.000 0.000
hsa-miR-521 0.000 0.000 0.000 0.000
hsa-miR-522 0.000 0.000 0.000 0.000
hsa-miR-523 0.000 0.000 0.000 0.000
hsa-miR-524 0.000 1.442 0.000 0.000
hsa-miR-524* 0.000 0.000 0.000 0.000
hsa-miR-525 0.000 0.000 0.000 0.000
hsa-miR-525* 0.000 0.000 0.000 0.000
hsa-miR-526a 0.000 0.000 0.000 0.000
hsa-miR-526b 0.000 0.000 0.000 0.000
hsa-miR-526b* 0.000 0.000 0.000 0.000
hsa-miR-526c 0.000 0.000 0.000 0.000
hsa-miR-527 0.000 0.000 0.000 0.000
mmu-miR-129-2 0.000 0.000 0.000 0.000
mmu-miR-137 0.000 5.496 13.293 297.839
mmu-miR-224 0.000 14.446 18.831 0.000
mmu-miR-424 0.000 0.000 0.000 0.000
215
APPENDIX C
DATA RELEVANT TO CHAPTER 5
216
Lung Cancer
miRNA H23 H522 HOP62 A549 H719
let-7a-1* 265.95 179.87 207.33 185.52 161.16
let-7a-2-P 955.49 689.95 6963.23 2285.83 2472.69
let-7a-3* 33.08 83.42 211.59 93.59 156.54
let-7b* 39.04 51.46 315.75 193.40 111.64
let-7c* 6.88 211.35 0.00 7.26 16.56
let-7d* 758.26 892.58 531.53 559.29 518.95
let-7e* 11.11 8.41 8.54 13.67 8.44
let-7f-1* 604.63 767.32 536.23 407.61 566.88
let-7f-2* 99.43 152.62 146.06 28.71 60.48
let-7g* 240.90 216.06 265.32 210.99 229.67
let-7i* 192.47 103.50 302.70 397.01 61.15
MIR-001-1 235.74 237.72 150.40 161.25 158.32
MIR-001-2 280.89 489.68 206.65 230.54 201.40
MIR-007-1 102.12 63.49 74.22 33.50 106.96
MIR-007-2 41.92 135.67 54.82 131.72 172.50
MIR-007-3 131.37 80.56 30.97 18.73 38.89
MIR-009-1-P 5597.95 3393.11 1115.78 2307.45 2675.05
MIR-009-2-P 2581.00 2242.82 1550.28 1797.72 3018.59
MIR-009-3-P 80.10 67.50 42.41 45.62 58.83
MIR-010a,b 12.71 19.45 295.67 99.21 97.38
MIR-015a 481.02 329.27 431.62 171.29 309.16
MIR-015b 1702.45 907.46 1615.58 633.01 492.64
MIR-016-1 1089.23 372.69 1273.77 562.81 658.91
MIR-016-2 1004.04 979.67 946.32 721.99 474.10
MIR-017-P 2942.14 2181.34 2119.10 1400.82 1987.48
MIR-018 3558.02 1840.52 3488.41 1989.36 3561.25
MIR-019a-P 720.25 596.47 808.46 391.81 1115.88
MIR-019b-1-P 1685.67 1948.48 534.60 913.41 1423.75
MIR-019b-2-P 7.91 252.37 7.56 7.44 0.00
MIR-020 8.33 15.47 86.39 4.59 13.58
MIR-021 34307.94 28445.73 56698.21 28133.73 7919.58
MIR-022-P 687.51 630.50 1131.34 686.01 431.19
MIR-023a 772.08 663.06 395.42 522.18 577.20
MIR-023b-P 1643.49 1054.85 1133.81 2111.67 1385.47
Continued
Table C.1. microRNA precursor expression in 37 different human cancer cell
lines. All data was generated using real-time PCR and SYBR green detection except
(*) which was assayed using TaqMan probes. Data is presented as copies of
microRNA per cell. (P) Real-time PCR was performed on the microRNA primary
precursor.
217
Table C.1. continued
MIR-024-1,2 7040.69 6254.60 7985.92 19753.19 4869.47
MIR-025 1099.19 1253.20 378.54 503.76 616.32
MIR-026a-1,2 280.92 181.99 163.98 153.29 148.84
MIR-026b 2145.20 2227.08 1214.05 1237.43 1671.01
MIR-027a, b 1910.70 1729.11 1008.48 864.06 1033.30
MIR-028 28.26 57.34 37.84 38.49 33.75
MIR-029a,c 818.00 162.66 629.82 227.71 380.48
MIR-029b-1,2 43.77 37.08 9.39 66.72 72.46
MIR-030a,e 522.59 1376.50 507.33 425.58 487.65
MIR-030b 1425.69 824.97 701.24 758.20 677.09
MIR-030c-1,2 1199.30 1143.95 600.29 529.57 1072.09
MIR-030d 239.05 100.49 77.00 87.62 76.41
MIR-031-P 8055.97 7666.13 3031.34 4014.24 4909.87
MIR-032 101.94 177.39 103.16 40.81 138.36
MIR-033 17.73 34.24 19.36 11.76 4.61
MIR-034a 544.66 1046.52 336.85 415.98 583.76
MIR-034b 122.55 79.37 87.76 107.06 94.86
MIR-034c 0.00 0.00 0.00 0.00 0.00
MIR-092-1 54.63 46.45 52.81 5.84 32.67
MIR-092-2-P 457.29 2077.18 157.23 203.76 300.75
MIR-093 1197.11 879.23 394.02 593.41 603.87
MIR-095 7.57 3.93 3.12 2.80 5.47
MIR-096-P 28318.18 23105.14 14521.64 22762.64 21271.33
MIR-098 5020.89 6762.98 4878.86 2605.99 2730.18
MIR-099a 85.21 147.46 106.00 89.92 48.01
MIR-099b 1778.78 1931.36 2476.97 2059.50 1699.39
MIR-100 66.86 174.68 7120.22 1396.05 2294.07
MIR-101-1 52.98 33.33 13.28 16.77 37.39
MIR-101-2 382.15 292.62 115.17 392.38 326.67
MIR-103-1,2 4723.29 4433.55 5005.15 3179.53 3623.14
MIR-105-1,2 103.87 209.00 173.88 144.27 267.53
MIR-106a-P 85.40 488.85 56.22 74.68 110.61
MIR-106b 2137.52 2795.99 1025.55 1011.34 2039.91
MIR-107 4995.26 6507.37 7196.86 4386.27 4628.41
MIR-108 635.56 390.56 401.27 264.16 391.74
MIR-122a 119.27 44.69 50.22 67.22 99.48
MIR-124a-1,2,3 157.84 55.04 65.61 59.89 130.66
MIR-125a 1038.26 709.12 806.27 969.24 586.48
MIR-125b-1 56.32 192.33 912.40 63.85 967.47
MIR-125b-2 130.81 291.63 73.80 68.32 126.51
MIR-126 132.34 56.15 48.61 122.86 173.58
Continued
218
Table C.1. continued
MIR-127 4108.54 4358.95 3771.20 4154.41 4551.32
MIR-128a 546.67 589.97 417.97 328.73 520.99
MIR-128b 81.00 92.31 50.64 91.92 74.19
MIR-129-2 6335.68 5503.13 3261.62 6108.57 7099.30
MIR-130a-P 7812.21 5720.04 3814.80 4229.86 5197.07
MIR-130b 82.44 135.93 180.25 62.54 126.19
MIR-132 36.31 28.02 36.29 18.47 23.96
MIR-133a-1,2 1086.70 2412.47 367.12 628.16 1392.66
MIR-133b 1637.94 2096.22 1127.32 1450.87 1685.24
MIR-134 3819.51 2658.98 1733.16 1950.82 2164.33
MIR-135a-1,2 131.72 19.10 20.53 109.86 18.81
MIR-135b-P 284.95 229.48 121.78 60.20 229.33
MIR-136 300.20 56.44 32.38 59.31 96.91
MIR-137* 31.37 496.85 37.78 54.93 133.17
MIR-138-1,2 28.52 55.93 12.11 20.99 59.51
MIR-139 380.22 428.01 398.54 238.36 317.64
MIR-140 2.33 6.23 0.00 1.11 0.00
MIR-141 162.30 90.81 41.16 87.45 46.51
MIR-142-P 2269.21 2887.45 1558.12 1324.71 876.08
MIR-143 381.69 296.83 163.91 220.85 193.39
MIR-144 7.59 7.56 14.05 21.42 7.86
MIR-145-P 657.81 298.35 331.74 261.49 283.33
MIR-146 27.13 16.99 52.65 18.50 10.59
MIR-147 119.02 75.31 65.69 115.36 122.04
MIR-148a-P 86.97 96.69 68.42 60.80 103.86
MIR-148b-P 795.38 454.00 374.21 488.03 706.18
MIR-149 2139.25 1537.53 1175.00 958.50 1508.87
MIR-150 708.53 597.52 368.56 347.51 483.59
MIR-151 269.79 324.81 97.88 214.02 261.14
MIR-152 53.98 90.93 63.91 45.83 41.55
MIR-153-1,2 68.99 43.96 66.13 17.66 100.98
MIR-154 64.37 48.57 55.44 20.30 52.56
MIR-155 104.58 54.75 387.31 27.19 115.75
MIR-181a,c 462.74 1586.52 788.12 45.19 327.79
MIR-181b-1,2 170.82 105.22 478.39 51.93 119.33
MIR-182 16782.47 11635.17 3098.97 3692.70 5421.11
MIR-183 422.42 502.89 86.79 68.75 201.35
MIR-184 1277.07 2593.48 254.41 4276.10 1563.48
MIR-185 4185.25 3742.88 2618.53 3127.12 4049.31
MIR-186-P 2363.83 3071.69 1342.94 1535.31 2276.32
MIR-187 176.31 88.73 65.65 112.54 97.52
Continued
219
Table C.1. continued
MIR-188b-P 247.95 169.92 123.15 135.49 240.23
MIR-190 4.19 4.47 1.54 1.49 2.73
MIR-191 79.51 35.34 20.38 13.27 10.44
MIR-192 862.58 1238.62 398.72 162.03 419.74
MIR-193 461.51 213.56 195.89 121.91 203.39
MIR-194-1,2 128.92 102.70 49.43 78.38 73.32
MIR-195-P 11048.43 9480.00 5569.01 5961.99 8309.40
MIR-196a-1 333.47 273.37 318.43 342.37 372.13
MIR-196a-2 403.49 718.06 254.52 218.08 523.36
MIR-196b 1886.11 485.29 1721.86 1332.71 244.78
MIR-197 7737.92 5623.72 5846.92 6877.23 6379.99
MIR-198 121.41 421.77 1855.34 107.09 61.85
MIR-199a-1-P 4603.30 3755.66 3064.37 4221.39 3742.74
MIR-199a-2-P 388.19 892.72 205.15 329.86 448.33
MIR-199b-P 3821.25 3086.45 2398.82 2164.54 2520.14
MIR-200a-P 48724.36 103362.05 52243.79 88095.16 79423.16
MIR-200b 70.31 61.37 33.90 42.31 66.46
MIR-200c 76.02 98.52 70.05 107.34 80.79
MIR-203 50.97 57.56 24.18 37.76 4.40
MIR-204 2340.50 2050.83 1150.18 1039.84 237.25
MIR-205 151.63 42.09 23.46 8.78 4.00
MIR-206 157.92 99.63 94.43 149.39 84.66
MIR-208 168.49 101.77 42.39 75.17 71.40
MIR-210 19101.01 8854.71 11551.43 6828.08 9473.77
MIR-211 5349.76 3265.12 3061.06 3179.64 3884.94
MIR-212 495.65 372.03 581.62 250.42 554.22
MIR-213 388.37 75.83 456.26 170.08 198.73
MIR-214 2472.16 2248.12 1468.28 1950.73 2392.13
MIR-215 320.99 234.47 202.01 59.81 112.19
MIR-216 349.64 324.80 202.14 189.70 294.41
MIR-217 14.59 3.70 3.34 5.15 0.88
MIR-218-1,2 32.87 109.99 204.29 13.90 26.48
MIR-219-1,2 149.47 84.22 56.01 60.13 72.36
MIR-220 557.37 260.42 266.69 296.23 386.24
MIR-221 93.53 726.01 381.65 129.43 234.46
MIR-222-P 240.53 984.95 258.50 180.07 363.07
MIR-223 82.00 92.44 41.17 61.27 99.10
MIR-224 436.36 293.11 579.04 400.99 276.96
MIR-296 401.58 391.90 235.52 304.70 329.08
MIR-299-P 1718.54 1038.23 902.64 857.70 294.06
MIR-301 452.34 1335.66 738.04 409.57 563.00
Continued
220
Table C.1. continued
MIR-302 83.93 61.08 56.91 44.35 61.45
MIR-302b 217.14 168.72 91.18 157.19 64.78
MIR-302c 208.27 111.79 159.48 190.25 151.05
MIR-302d 245.29 173.45 140.30 167.43 196.37
MIR-320 457.64 508.20 244.78 328.84 342.20
MIR-321 9128.86 25372.00 4610.81 4473.53 5045.22
MIR-323 702.28 180.75 293.73 377.52 200.13
MIR-324-P 1061.12 864.04 525.11 578.77 679.76
MIR-325 52.16 75.95 37.19 37.46 69.16
MIR-326-P 121.32 75.59 24.12 57.81 40.66
MIR-328 2926.73 2635.76 1361.21 2047.73 1998.69
MIR-330 647.99 502.23 405.89 342.33 218.09
MIR-331-P 626.40 605.77 319.12 412.74 695.55
MIR-335 13.65 21.22 11.49 6.96 9.01
MIR-337 764.55 1013.00 467.62 523.29 821.16
MIR-338 1296.14 824.71 601.20 698.75 853.24
MIR-339 1894.43 877.10 1113.03 847.31 913.17
MIR-340 1277.88 1113.02 303.39 387.71 775.69
MIR-342 556.25 373.72 281.70 281.32 400.09
MIR-345 2542.22 2499.85 1452.14 1474.08 2096.17
MIR-346 3166.03 2632.09 2434.64 2407.84 2923.48
MIR-361 72.23 195.27 180.72 81.02 184.98
MIR-367 48.67 12.90 21.68 14.78 24.06
MIR-368 3.20 0.00 1.67 2.56 4.74
MIR-369 0.00 0.00 0.00 0.00 0.00
MIR-370 392.03 254.26 202.93 203.30 167.93
MIR-371 2053.40 1491.35 988.15 1038.79 1354.55
MIR-372 25.94 5.48 9.64 19.80 11.66
MIR-373 415.26 270.11 168.62 212.80 316.57
MIR-374-P 6.47 36.55 78.86 24.40 52.41
MIR-375 60.68 68.16 58.33 73.47 83.37
MIR-376 594.09 75.43 332.41 88.43 21.39
MIR-377 210.11 322.03 86.72 103.31 235.85
MIR-378 144.84 123.16 44.99 30.91 65.25
MIR-379 256.34 10.46 148.76 44.74 5.89
MIR-380 119.87 6.60 68.91 2.79 43.51
MIR-381 7.49 21.78 20.95 26.01 22.36
MIR-382 40.07 27.73 31.35 31.30 28.88
MIR-383 99.97 277.46 63.06 107.91 121.73
MIR-384 6.59 0.00 16.86 5.30 2.49
MIR-422b 926.07 731.49 546.45 636.06 791.78
Continued
221
Table C.1 continued
MIR-423 425.22 588.86 234.32 248.33 348.79
MIR-424 155.90 45.19 145.28 46.28 25.17
MIR-425 306.97 141.99 154.45 119.62 139.43
Pancreas Cancer
miRNA PANC-1 HS766T MIAPACA-2 MPANC-96 PL45
let-7a-1* 16.80 30.16 80.27 102.05 84.19
let-7a-2-P 3224.23 2598.29 485.50 435.56 832.20
let-7a-3* 38.96 3.97 22.84 105.76 61.93
let-7b* 182.96 76.51 14.52 25.13 8.60
let-7c* 0.74 0.00 0.00 0.61 0.00
let-7d* 194.21 509.85 243.10 429.99 290.02
let-7e* 5.20 9.34 0.00 3.94 5.43
let-7f-1* 28.23 93.16 165.28 349.71 252.98
let-7f-2* 27.53 6.81 12.47 27.87 50.54
let-7g* 149.76 255.29 83.77 145.50 55.71
let-7i* 46.10 126.67 185.79 442.66 47.93
MIR-001-1 251.23 226.75 116.54 48.23 63.46
MIR-001-2 337.41 356.14 435.89 167.46 172.93
MIR-007-1 104.29 141.97 129.95 108.03 84.75
MIR-007-2 190.09 172.16 46.99 26.52 24.50
MIR-007-3 52.92 42.94 15.29 2.78 8.30
MIR-009-1-P 5105.28 3018.80 1237.39 677.78 861.29
MIR-009-2-P 6453.36 5756.35 986.62 525.86 954.43
MIR-009-3-P 93.83 100.03 7.78 5.26 15.84
MIR-010a,b 514.30 65.33 53.37 171.07 109.37
MIR-015a 387.34 328.62 86.78 123.78 76.05
MIR-015b 650.08 420.14 1187.20 485.70 938.99
MIR-016-1 338.96 460.77 553.75 304.77 244.89
MIR-016-2 1157.98 1151.18 500.66 259.69 544.29
MIR-017-P 1679.82 2033.84 1234.19 1514.81 700.23
MIR-018 1171.58 5037.96 3329.06 2620.18 706.56
MIR-019a-P 427.41 2026.17 904.64 714.08 232.12
MIR-019b-1-P 301.25 1360.53 340.09 260.51 215.90
MIR-019b-2-P 8.23 3.46 0.00 1.09 0.00
MIR-020 3.25 19.76 21.06 17.08 21.65
MIR-021 16468.89 27976.87 5043.89 7916.38 2341.17
MIR-022-P 749.33 612.63 1034.38 2528.56 215.26
MIR-023a 1158.52 1093.45 318.07 647.63 286.88
MIR-023b-P 1343.30 1527.30 482.98 707.53 570.17
MIR-024-1,2 4140.45 3128.31 3298.33 10074.36 1587.91
Continued
222
Table C.1. continued
MIR-025 595.61 993.94 929.65 732.37 517.66
MIR-026a-1,2 213.56 200.13 59.50 119.08 75.06
MIR-026b 1900.16 1937.29 392.53 401.30 490.37
MIR-027a, b 1398.27 1604.13 472.12 549.12 260.12
MIR-028 66.18 27.90 18.22 3.04 23.26
MIR-029a,c 345.25 1978.11 820.59 667.62 650.14
MIR-029b-1,2 27.34 46.08 1.58 5.03 1.92
MIR-030a,e 462.14 357.11 719.91 120.96 291.94
MIR-030b 1242.58 1536.38 364.69 409.69 334.30
MIR-030c-1,2 1086.79 610.22 151.54 133.75 166.60
MIR-030d 23.98 30.84 53.45 141.11 57.46
MIR-031-P 5334.83 5125.43 2039.01 768.53 609.39
MIR-032 76.77 268.26 314.47 177.62 151.11
MIR-033 37.08 17.28 3.54 6.38 6.29
MIR-034a 146.47 393.56 124.27 84.51 123.53
MIR-034b 117.44 154.72 21.85 7.67 12.63
MIR-034c 0.00 0.00 0.00 0.00 0.00
MIR-092-1 41.15 85.43 88.37 93.50 27.73
MIR-092-2-P 616.46 430.73 71.21 21.71 42.21
MIR-093 947.82 899.78 302.75 593.54 736.90
MIR-095 13.67 11.37 4.26 0.89 8.66
MIR-096-P 22929.50 22068.04 7178.77 4269.90 7181.80
MIR-098 4664.63 6798.12 2452.58 2121.58 3822.24
MIR-099a 78.01 76.20 14.46 7.90 17.44
MIR-099b 435.56 415.90 879.21 650.58 1206.24
MIR-100 2347.78 4542.34 11.38 165.42 195.83
MIR-101-1 62.76 35.56 13.37 21.31 14.38
MIR-101-2 245.76 510.76 188.16 136.10 99.40
MIR-103-1,2 3827.33 4354.49 1896.03 1503.21 1668.69
MIR-105-1,2 252.64 268.23 74.99 61.55 136.68
MIR-106a-P 127.24 134.34 14.27 17.99 14.43
MIR-106b 894.55 2395.23 714.09 845.66 354.84
MIR-107 3102.02 4619.59 2977.95 2430.28 1731.29
MIR-108 428.70 313.32 158.55 76.86 278.99
MIR-122a 141.23 109.58 6.49 6.32 12.54
MIR-124a-1,2,3 141.37 128.07 18.05 15.05 14.47
MIR-125a 964.05 355.50 155.07 142.32 202.67
MIR-125b-1 338.68 182.28 17.19 2.62 27.89
MIR-125b-2 112.11 168.42 57.72 12.53 97.15
MIR-126 81.79 213.49 80.73 14.69 16.83
MIR-127 7629.42 7423.07 3033.12 2613.57 2857.53
Continued
223
Table C.1. continued
MIR-128a 312.49 249.31 277.34 174.23 337.89
MIR-128b 180.21 131.35 55.59 32.17 27.83
MIR-129-2 8536.54 6627.53 2897.67 1307.01 2660.70
MIR-130a-P 10779.62 7692.85 2076.75 1011.86 1804.38
MIR-130b 124.56 116.06 58.71 54.40 7.93
MIR-132 15.48 1.07 7.31 13.20 1.73
MIR-133a-1,2 737.55 714.50 303.41 205.98 153.94
MIR-133b 1784.71 1009.31 474.43 339.96 398.17
MIR-134 3577.31 5073.56 319.55 201.52 404.63
MIR-135a-1,2 118.75 141.54 46.59 258.97 10.76
MIR-135b-P 395.54 159.11 81.24 39.32 135.23
MIR-136 127.04 64.97 91.82 4.26 5.39
MIR-137* 23.11 13.12 732.14 5.15 20.71
MIR-138-1,2 71.74 99.85 15.14 3.19 1.88
MIR-139 2651.48 2297.95 397.97 203.42 256.13
MIR-140 1.84 2.94 0.00 0.00 0.00
MIR-141 144.87 68.46 42.22 38.95 59.59
MIR-142-P 4806.11 4593.64 750.51 333.19 810.57
MIR-143 426.73 362.91 136.39 92.43 111.77
MIR-144 14.48 0.83 0.00 0.00 0.00
MIR-145-P 791.29 573.19 95.47 64.08 71.19
MIR-146 28.71 10.29 3.97 5.02 4.55
MIR-147 204.40 176.46 50.63 2.59 47.94
MIR-148a-P 106.46 113.20 41.67 28.00 42.14
MIR-148b-P 463.60 347.16 162.93 189.26 114.46
MIR-149 2291.53 2196.33 594.82 227.59 314.20
MIR-150 717.58 761.04 318.83 159.78 342.11
MIR-151 228.69 343.08 40.51 72.90 45.19
MIR-152 102.30 153.79 15.02 31.76 25.82
MIR-153-1,2 75.13 132.39 50.90 306.99 6.07
MIR-154 113.29 86.90 6.08 0.99 25.27
MIR-155 99.47 530.61 29.13 46.07 79.94
MIR-181a,c 327.53 236.16 90.85 160.01 42.26
MIR-181b-1,2 178.62 71.92 25.34 73.09 90.05
MIR-182 2597.90 4113.66 6055.84 3821.98 1983.74
MIR-183 195.69 112.40 86.11 105.71 50.77
MIR-184 2133.69 1825.81 915.01 251.44 876.48
MIR-185 4174.82 3893.69 1666.88 1183.49 1828.01
MIR-186-P 2386.55 2616.25 2133.64 1665.63 1125.64
MIR-187 170.61 70.40 31.61 11.71 39.73
MIR-188b-P 267.59 281.95 34.93 15.24 23.56
Continued
224
Table C.1. continued
MIR-190 9.17 2.55 0.94 0.00 5.78
MIR-191 86.46 34.80 3.67 3.73 3.38
MIR-192 679.86 622.21 220.12 57.31 140.92
MIR-193 382.66 271.51 75.82 9.09 30.67
MIR-194-1,2 107.22 99.09 21.62 16.59 22.37
MIR-195-P 10184.74 3921.09 2089.51 877.81 1542.37
MIR-196a-1 527.38 479.25 195.36 48.51 165.50
MIR-196a-2 419.99 208.52 101.33 143.21 73.38
MIR-196b 1556.51 5768.30 211.30 8447.67 815.01
MIR-197 7374.37 20361.72 3865.80 3234.06 3858.07
MIR-198 44.25 3.06 32.50 3.96 179.03
MIR-199a-1-P 4602.43 4865.89 1553.04 1223.57 2279.62
MIR-199a-2-P 464.20 472.84 151.10 58.18 126.59
MIR-199b-P 3772.77 3593.00 1356.90 458.55 984.85
MIR-200a-P 77829.22 87456.35 35921.32 17962.89 29588.61
MIR-200b 159.84 130.10 89.82 48.14 58.82
MIR-200c 231.37 166.10 20.95 6.75 63.41
MIR-203 40.63 40.31 1.36 13.18 3.06
MIR-204 2445.34 3176.55 507.60 290.60 456.56
MIR-205 25.27 40.26 4.76 1.28 66.41
MIR-206 292.28 196.19 53.63 15.28 34.04
MIR-208 180.37 157.95 41.88 23.66 22.92
MIR-210 19716.38 27549.84 2112.05 783.66 3317.95
MIR-211 4656.25 3962.70 1705.99 876.28 1596.53
MIR-212 1248.99 2553.89 326.20 218.21 125.20
MIR-213 352.55 392.45 37.89 151.21 75.97
MIR-214 2740.06 2836.55 788.21 520.13 566.40
MIR-215 76.03 129.69 155.46 148.84 286.85
MIR-216 435.20 312.39 143.03 54.73 144.02
MIR-217 13.20 16.73 9.12 1.40 2.57
MIR-218-1,2 58.13 44.94 18.72 4.75 18.01
MIR-219-1,2 56.75 104.27 11.30 32.94 13.26
MIR-220 445.07 431.98 258.20 59.70 54.95
MIR-221 158.08 757.66 559.65 292.04 212.95
MIR-222-P 307.07 492.27 524.06 295.62 327.56
MIR-223 75.12 136.83 34.79 22.46 15.57
MIR-224 245.73 253.89 133.58 408.99 186.77
MIR-296 312.41 22.74 54.89 24.76 57.00
MIR-299-P 1836.62 1500.70 233.10 209.32 202.10
MIR-301 174.26 371.17 131.33 60.20 160.48
MIR-302 54.28 77.40 7.65 52.66 39.67
Continued
225
Table C.1. continued
MIR-302b 520.66 516.42 72.38 49.60 140.70
MIR-302c 253.69 258.04 57.01 65.75 45.71
MIR-302d 126.05 225.74 63.41 7.65 48.51
MIR-320 696.86 708.39 170.07 135.95 109.16
MIR-321 12134.67 7705.12 2470.84 4737.19 2037.01
MIR-323 175.48 304.13 91.09 86.67 86.08
MIR-324-P 1076.04 844.50 229.60 208.45 200.66
MIR-325 135.51 52.21 34.97 21.48 41.11
MIR-326-P 191.02 158.74 19.10 14.88 10.14
MIR-328 3172.97 4674.27 812.12 370.93 647.54
MIR-330 647.86 524.61 147.57 68.76 60.28
MIR-331-P 513.45 643.32 246.97 128.98 255.50
MIR-335 7.48 17.36 36.05 3.51 52.44
MIR-337 912.91 811.17 149.01 76.60 167.11
MIR-338 1458.57 1077.86 132.11 97.54 170.11
MIR-339 2002.03 1150.47 602.58 336.10 337.36
MIR-340 339.22 520.09 552.58 333.78 162.90
MIR-342 583.60 509.75 159.89 84.76 143.83
MIR-345 4257.07 4615.43 1006.64 333.64 882.09
MIR-346 3351.71 3227.02 3171.48 2880.92 3152.52
MIR-361 219.09 181.19 79.96 107.82 119.63
MIR-367 5.44 17.67 0.00 53.63 48.87
MIR-368 7.88 5.18 2.61 0.00 0.00
MIR-369 13.90 3.45 0.00 0.97 0.00
MIR-370 601.31 326.88 62.08 11.25 8.25
MIR-371 2705.17 1962.26 380.54 182.67 366.35
MIR-372 16.00 13.32 0.00 3.21 1.64
MIR-373 611.83 561.73 55.94 31.94 72.01
MIR-374-P 73.51 99.95 8.52 29.70 19.69
MIR-375 9.26 127.24 35.51 5.75 15.27
MIR-376 697.96 39.00 4.78 1.00 2.35
MIR-377 475.60 412.51 118.61 58.77 60.48
MIR-378 143.60 231.34 42.58 83.80 53.25
MIR-379 307.56 3.88 12.20 3.08 2.68
MIR-380 142.90 20.94 10.54 1.00 2.11
MIR-381 97.30 55.55 1.05 2.13 6.99
MIR-382 63.96 28.83 10.70 15.00 7.04
MIR-383 216.08 189.57 8.07 14.23 22.44
MIR-384 3.27 2.20 0.61 0.86 1.21
MIR-422b 801.27 785.33 243.67 156.91 234.92
MIR-423 457.69 383.86 54.05 30.00 41.45
Continued
226
Table C.1. continued
MIR-424 37.06 87.89 4.59 27.30 4.46
MIR-425 120.59 34.51 66.67 37.57 18.30
Prostate Cancer
miRNA PC3 PPC1 DU145 TSUPR1 LNCAP
let-7a-1* 143.19 3.38 13.13 43.56 11.49
let-7a-2-P 7803.95 1821.31 1279.00 270.94 1081.41
let-7a-3* 468.33 41.00 16.76 15.01 168.71
let-7b* 362.67 214.51 115.40 37.70 633.87
let-7c* 0.00 1.98 0.00 6.95 696.73
let-7d* 250.35 84.65 133.10 139.25 182.83
let-7e* 10.25 2.62 2.06 0.00 14.23
let-7f-1* 189.51 17.10 6.48 38.59 100.43
let-7f-2* 25.79 37.07 16.86 12.57 4.84
let-7g* 213.29 41.25 26.16 137.57 172.53
let-7i* 195.52 82.13 85.05 89.15 76.55
MIR-001-1 89.47 0.61 182.82 7.01 323.81
MIR-001-2 490.26 466.93 791.41 103.47 432.13
MIR-007-1 102.00 2.16 107.86 1.69 175.44
MIR-007-2 31.22 3.32 66.10 1.94 80.76
MIR-007-3 12.26 4.26 20.27 3.93 78.76
MIR-009-1-P 1421.70 1676.30 1834.68 761.10 2469.69
MIR-009-2-P 791.18 638.66 1737.68 769.99 4470.95
MIR-009-3-P 17.74 9.25 31.99 5.10 38.68
MIR-010a,b 177.69 0.00 44.03 3.81 12.53
MIR-015a 138.46 75.13 193.41 27.99 163.98
MIR-015b 801.98 526.38 440.52 192.09 700.09
MIR-016-1 692.86 213.80 444.17 1258.48 1097.20
MIR-016-2 476.06 289.82 680.06 136.66 547.78
MIR-017-P 278.14 372.46 537.63 176.51 911.49
MIR-018 1843.05 1591.29 838.76 2140.52 5244.02
MIR-019a-P 261.29 53.79 57.81 31.05 1394.49
MIR-019b-1-P 519.82 206.98 698.10 175.33 999.42
MIR-019b-2-P 2.57 0.00 0.00 0.00 4.55
MIR-020 0.99 0.00 1.94 0.00 24.87
MIR-021 27307.07 16179.01 26034.95 58978.05 11180.51
MIR-022-P 847.47 224.08 228.01 312.93 1998.11
MIR-023a 384.12 93.54 451.50 122.45 489.04
MIR-023b-P 697.38 141.92 1156.37 292.68 1639.94
MIR-024-1,2 3192.41 2824.37 6164.42 4829.63 1930.89
MIR-025 120.90 248.46 334.29 192.51 583.07
Continued
227
Table C.1. continued
MIR-026a-1,2 67.86 30.10 137.99 5.53 182.86
MIR-026b 618.60 146.02 702.08 139.92 1126.24
MIR-027a, b 627.34 286.11 958.46 261.29 1245.07
MIR-028 18.74 11.17 19.72 24.97 39.45
MIR-029a,c 389.26 117.98 1554.76 687.60 78.84
MIR-029b-1,2 4.59 13.41 22.10 0.00 16.71
MIR-030a,e 101.61 42.71 204.39 42.16 386.97
MIR-030b 660.66 297.34 639.77 217.38 839.65
MIR-030c-1,2 497.20 180.53 551.17 101.24 541.49
MIR-030d 105.90 50.66 37.70 1.45 101.11
MIR-031-P 2897.58 2220.49 3777.19 1207.00 3333.38
MIR-032 84.70 63.31 362.60 86.38 172.04
MIR-033 1.29 2.64 1.94 4.14 7.67
MIR-034a 159.55 92.83 237.03 75.04 714.64
MIR-034b 30.25 30.26 43.46 18.08 129.86
MIR-034c 0.00 0.00 0.00 0.00 0.00
MIR-092-1 2.29 0.65 1.29 1.08 22.82
MIR-092-2-P 102.50 19.82 128.43 8.26 263.51
MIR-093 237.81 73.29 258.23 77.70 555.98
MIR-095 35.73 0.00 1.66 0.00 17.20
MIR-096-P 8420.23 7080.78 7725.59 4131.95 16119.55
MIR-098 3064.95 9591.90 3002.87 5470.93 11417.13
MIR-099a 50.42 24.17 13.15 18.92 113.79
MIR-099b 719.48 143.94 256.80 203.49 350.12
MIR-100 27721.96 10716.83 2443.09 3061.02 69.42
MIR-101-1 3.08 3.27 32.13 2.59 48.74
MIR-101-2 168.77 119.16 371.70 383.37 168.14
MIR-103-1,2 2255.30 870.77 2771.90 2628.59 3985.63
MIR-105-1,2 83.01 48.36 211.21 56.74 225.47
MIR-106a-P 50.46 5.49 29.26 5.54 91.88
MIR-106b 1140.59 1221.49 1037.03 1675.07 2317.89
MIR-107 4209.53 1366.77 3687.58 6232.79 4434.74
MIR-108 161.06 78.38 316.94 72.05 237.81
MIR-122a 16.74 8.61 51.57 0.90 48.63
MIR-124a-1,2,3 23.04 46.82 19.87 29.09 59.03
MIR-125a 519.27 324.47 592.44 125.99 940.32
MIR-125b-1 1639.63 572.43 199.41 247.66 12.65
MIR-125b-2 47.70 47.20 73.46 4.30 489.56
MIR-126 38.85 2.20 98.54 12.94 54.94
MIR-127 3596.37 525.31 2803.02 383.88 3797.87
MIR-128a 450.87 245.01 463.95 487.28 708.52
Continued
228
Table C.1. continued
MIR-128b 59.26 22.24 53.68 15.25 107.76
MIR-129-2 3557.64 1015.30 3696.52 770.40 6436.40
MIR-130a-P 3014.93 1341.66 3706.16 925.38 4999.61
MIR-130b 29.91 30.59 22.24 4.07 126.40
MIR-132 0.63 0.75 28.06 36.09 62.30
MIR-133a-1,2 359.43 135.31 546.52 65.56 559.46
MIR-133b 451.32 321.68 642.65 141.05 1117.14
MIR-134 1074.84 478.97 1419.85 375.11 3132.08
MIR-135a-1,2 65.92 11.93 39.76 31.24 115.13
MIR-135b-P 89.87 89.04 148.00 25.55 111.23
MIR-136 80.15 7.37 55.13 5.46 44.03
MIR-137* 73.09 0.99 12.03 420.03 24.67
MIR-138-1,2 6.61 3.58 12.99 0.86 50.24
MIR-139 605.63 333.74 849.31 198.32 609.30
MIR-140 0.00 0.00 0.00 0.00 1.53
MIR-141 34.75 1.89 49.50 1.37 151.39
MIR-142-P 598.68 460.07 894.10 493.80 907.11
MIR-143 117.78 48.04 184.77 35.76 350.82
MIR-144 0.00 0.00 36.75 0.00 1.15
MIR-145-P 147.38 62.53 241.61 41.59 218.53
MIR-146 19.57 0.00 11.11 0.00 7.47
MIR-147 53.80 15.65 87.62 49.44 169.96
MIR-148a-P 52.29 4.30 36.68 3.01 41.99
MIR-148b-P 372.22 135.92 358.94 13.26 636.13
MIR-149 635.84 366.13 506.15 323.25 425.02
MIR-150 221.82 454.15 296.94 339.28 539.24
MIR-151 47.71 30.94 64.82 29.58 174.25
MIR-152 30.27 4.37 75.59 7.27 71.16
MIR-153-1,2 110.48 37.16 20.92 10.97 34.50
MIR-154 15.87 6.36 3.55 2.53 66.54
MIR-155 49.88 21.75 100.50 0.00 34.19
MIR-181a,c 513.58 373.99 674.73 1013.07 570.72
MIR-181b-1,2 28.29 44.54 100.55 68.09 63.61
MIR-182 19259.56 3736.58 5289.56 2424.54 6254.87
MIR-183 2185.76 31.11 115.86 21.08 165.81
MIR-184 790.67 450.90 1090.14 308.72 1901.97
MIR-185 1520.52 444.78 2044.93 382.46 2361.75
MIR-186-P 3362.29 1415.85 1178.33 663.57 5835.59
MIR-187 15.06 25.00 44.25 3.59 57.11
MIR-188b-P 34.55 17.24 75.31 15.91 149.05
MIR-190 0.00 0.00 13.47 0.00 4.49
Continued
229
Table C.1. continued
MIR-191 34.03 1.55 16.00 2.97 24.98
MIR-192 0.00 0.00 0.00 0.00 0.00
MIR-193 123.67 55.81 78.76 25.82 156.08
MIR-194-1,2 32.15 23.36 26.60 12.59 71.58
MIR-195-P 3566.94 3078.37 5740.18 1707.15 7202.21
MIR-196a-1 63.86 29.78 144.96 54.71 235.03
MIR-196a-2 209.86 161.48 217.71 71.71 247.93
MIR-196b 17440.37 7906.50 2621.91 6794.11 2220.79
MIR-197 11434.15 4772.09 6465.38 2466.05 3859.64
MIR-198 374.49 582.26 7.64 129.93 142.70
MIR-199a-1-P 1115.10 378.34 1855.95 303.14 1808.13
MIR-199a-2-P 150.70 122.14 151.10 25.18 206.67
MIR-199b-P 1261.58 1242.56 1802.80 485.85 1692.24
MIR-200a-P 33749.36 22042.83 26561.37 14966.42 71326.06
MIR-200b 44.61 7.59 29.23 2.77 145.67
MIR-200c 56.75 5.20 38.74 3.08 110.18
MIR-203 1.47 3.38 14.24 10.18 55.33
MIR-204 680.36 505.12 859.68 87.35 1466.30
MIR-205 23.76 5.41 57.73 9.72 58.22
MIR-206 54.13 24.04 45.58 13.47 165.32
MIR-208 15.48 26.00 63.55 35.44 84.71
MIR-210 2674.65 4916.21 5848.40 1665.02 9218.05
MIR-211 1303.52 1153.79 2765.18 899.53 4017.09
MIR-212 180.80 377.71 161.77 218.13 447.85
MIR-213 298.07 82.32 116.19 183.03 84.92
MIR-214 935.20 660.71 1158.28 430.22 1646.30
MIR-215 139.39 117.68 91.56 37.01 123.30
MIR-216 110.10 73.09 100.43 18.79 186.22
MIR-217 4.16 0.00 3.43 0.00 5.86
MIR-218-1,2 418.16 15.35 17.86 4.63 23.84
MIR-219-1,2 46.51 5.54 54.73 22.93 95.47
MIR-220 133.55 96.01 341.59 109.66 308.06
MIR-221 603.99 43.65 257.19 176.78 9.85
MIR-222-P 217.09 52.04 179.16 17.24 55.69
MIR-223 35.41 6.46 28.34 3.71 68.14
MIR-224 80.28 18.11 102.78 5.00 170.27
MIR-296 53.83 72.81 165.47 22.65 271.33
MIR-299-P 775.60 776.13 786.66 167.41 730.48
MIR-301 220.22 91.25 647.67 333.49 435.60
MIR-302 21.16 1.03 27.99 2.51 65.48
MIR-302b 89.25 10.61 128.93 6.75 85.94
Continued
230
Table C.1. continued
MIR-302c 61.09 10.91 106.79 5.17 85.09
MIR-302d 49.25 21.25 65.77 5.40 134.24
MIR-320 148.34 140.07 259.58 100.77 530.67
MIR-321 4727.31 5456.91 4070.12 7669.81 11284.62
MIR-323 103.81 19.90 136.03 61.73 136.41
MIR-324-P 281.42 302.40 496.47 144.67 508.54
MIR-325 71.26 4.78 13.98 8.34 25.91
MIR-326-P 20.25 12.95 25.77 2.64 28.13
MIR-328 839.85 563.54 1160.87 422.23 2225.28
MIR-330 225.25 187.22 202.35 45.33 515.13
MIR-331-P 213.19 103.34 263.55 59.11 470.40
MIR-335 2.83 6.95 25.08 5.44 2.17
MIR-337 158.62 127.84 327.55 38.22 578.33
MIR-338 300.39 85.36 538.28 88.57 378.56
MIR-339 992.34 257.97 630.54 258.69 1047.38
MIR-340 275.40 217.03 375.50 156.23 1234.53
MIR-342 108.53 72.67 149.55 42.10 233.95
MIR-345 696.32 460.56 1135.27 411.69 1954.75
MIR-346 1130.91 325.37 824.50 242.79 1555.54
MIR-361 111.41 55.01 42.87 178.79 9.16
MIR-367 18.01 0.00 5.55 27.26 84.53
MIR-368 1.90 0.00 1.80 0.00 0.00
MIR-369 0.00 0.00 17.06 0.00 0.00
MIR-370 114.48 64.86 146.39 46.64 162.50
MIR-371 454.50 454.08 915.52 265.16 807.42
MIR-372 5.77 0.72 1.70 1.33 3.47
MIR-373 70.22 49.74 153.42 25.31 289.97
MIR-374-P 8.93 0.00 0.00 1.42 15.88
MIR-375 36.15 53.13 14.69 65.38 9.93
MIR-376 21.81 9.84 0.70 10.86 2.67
MIR-377 63.20 102.88 5.07 184.44 3.77
MIR-378 62.81 22.56 5.59 75.57 32.68
MIR-379 10.53 1.41 0.00 2.30 0.85
MIR-380 0.00 9.15 0.00 2.48 0.00
MIR-381 4.34 3.81 2.02 11.42 1.66
MIR-382 14.20 21.58 4.17 47.78 0.00
MIR-383 26.00 31.59 8.60 95.19 75.27
MIR-384 0.00 0.00 0.00 0.00 0.00
MIR-422b 319.03 183.74 365.61 91.60 787.16
MIR-423 134.69 97.50 68.78 30.63 64.90
MIR-424 128.90 38.32 79.82 30.05 51.41
Continued
231
Table C.1. continued
MIR-425 1270.22 38.46 176.90 32.92 808.57
Head & Neck squamous cell carcinoma
miRNA SCC17A SCCD12 SCC10B SCC17B SCC5
let-7a-1* 97.56 47.14 85.66 9.29 114.80
let-7a-2-P 2181.48 1627.60 3047.02 677.51 2725.60
let-7a-3* 48.99 50.62 15.27 40.13 104.19
let-7b* 182.72 59.10 93.54 14.10 331.79
let-7c* 0.00 0.00 2.92 0.00 38.87
let-7d* 345.83 211.88 784.53 83.32 469.00
let-7e* 4.96 11.01 1.51 12.36 8.06
let-7f-1* 99.87 58.37 106.21 21.82 147.73
let-7f-2* 18.45 60.75 86.28 18.48 35.20
let-7g* 107.16 162.06 85.51 29.96 112.65
let-7i* 49.93 20.53 64.82 11.99 52.46
MIR-001-1 392.94 220.71 987.67 27.28 764.84
MIR-001-2 277.98 206.70 441.68 78.87 596.56
MIR-007-1 92.56 103.20 271.79 16.87 206.96
MIR-007-2 232.01 342.48 438.46 134.85 325.54
MIR-007-3 69.07 11.64 19.72 39.44 120.86
MIR-009-1-P 8684.55 20022.85 11202.45 11727.26 9435.17
MIR-009-2-P 6674.43 3045.53 10112.89 1060.81 6878.04
MIR-009-3-P 168.68 53.62 362.10 32.20 270.25
MIR-010a,b 27.62 6.59 101.83 5.02 86.28
MIR-015a 776.87 382.58 995.75 215.98 1354.60
MIR-015b 1255.74 1245.00 712.93 550.41 1381.27
MIR-016-1 755.82 544.73 532.42 278.58 1023.78
MIR-016-2 1246.09 1267.98 1776.64 1011.29 1927.71
MIR-017-P 1603.86 1489.17 2718.48 841.44 2868.79
MIR-018 1478.61 3390.66 1471.00 381.73 4950.29
MIR-019a-P 454.99 1046.33 608.93 100.91 2342.99
MIR-019b-1-P 1863.56 1605.28 3111.60 1995.79 3401.75
MIR-019b-2-P 16.14 15.11 7.79 1.06 6.43
MIR-020 9.71 6.81 27.84 3.46 7.98
MIR-021 18734.27 24011.81 47626.61 14564.60 34924.79
MIR-022-P 1070.96 559.58 1680.01 256.00 2087.32
MIR-023a 1624.96 1101.81 3336.22 746.34 1144.15
MIR-023b-P 2916.82 998.91 4747.52 517.43 4086.99
MIR-024-1,2 5952.26 8113.48 10823.30 6466.39 36802.72
MIR-025 945.74 369.86 1426.20 178.76 1076.87
MIR-026a-1,2 390.12 146.41 746.95 80.09 518.96
Continued
232
Table C.1. continued
MIR-026b 2710.92 988.23 6541.30 618.02 4084.45
MIR-027a, b 3032.99 898.39 5323.47 489.58 4219.40
MIR-028 153.57 41.40 120.23 46.08 210.64
MIR-029a,c 1059.72 542.59 746.53 317.15 623.57
MIR-029b-1,2 81.55 157.76 185.85 97.77 119.17
MIR-030a,e 551.97 244.24 1164.23 40.88 751.33
MIR-030b 1657.44 653.01 3347.95 338.52 2090.38
MIR-030c-1,2 1680.65 554.29 2886.48 247.58 2648.28
MIR-030d 38.30 23.30 169.64 1.13 74.68
MIR-031-P 13347.76 7342.21 22554.51 3398.07 18361.32
MIR-032 297.89 155.64 646.36 18.45 521.43
MIR-033 54.13 15.28 140.81 2.59 57.96
MIR-034a 1180.68 1256.29 1866.80 940.88 1963.02
MIR-034b 249.32 129.02 366.22 50.76 296.73
MIR-034c 0.00 0.00 0.00 0.00 0.00
MIR-092-1 36.20 78.10 80.81 13.42 164.98
MIR-092-2-P 436.53 168.57 1234.94 82.99 707.04
MIR-093 1143.10 1900.00 2190.31 1537.15 1459.50
MIR-095 20.13 6.81 64.55 4.02 7.08
MIR-096-P 23359.90 29188.11 34457.00 14239.61 31733.46
MIR-098 4764.91 4042.46 10246.63 1564.73 6956.44
MIR-099a 60.48 73.72 108.94 50.51 78.87
MIR-099b 427.35 741.58 574.89 983.86 351.67
MIR-100 540.14 4523.79 223.19 404.51 216.87
MIR-101-1 46.62 58.15 195.90 25.74 248.27
MIR-101-2 570.40 952.04 939.28 649.54 1673.92
MIR-103-1,2 4049.09 3479.62 6695.88 1931.93 5920.25
MIR-105-1,2 540.48 174.25 799.38 102.53 542.19
MIR-106a-P 209.74 128.27 419.22 43.81 335.08
MIR-106b 1460.17 1453.89 1683.50 1242.37 2058.86
MIR-107 4244.97 4207.71 6625.55 2942.94 6387.63
MIR-108 766.01 270.31 1271.85 181.27 946.23
MIR-122a 131.01 46.89 290.76 43.68 228.04
MIR-124a-1,2,3 183.49 127.47 423.20 144.37 215.31
MIR-125a 1188.83 542.18 2048.02 513.90 1483.59
MIR-125b-1 101.68 95.04 133.72 33.12 115.51
MIR-125b-2 227.94 107.49 546.85 32.49 292.52
MIR-126 139.81 58.30 149.61 264.35 130.80
MIR-127 7650.07 8157.38 8719.52 6955.59 10030.36
MIR-128a 851.90 8021.02 941.47 3298.28 661.52
MIR-128b 242.05 592.67 303.19 541.96 318.26
Continued
233
Table C.1. continued
MIR-129-2 11576.75 3555.18 22329.18 1561.83 14186.73
MIR-130a-P 11632.11 4613.82 23551.55 1716.34 14632.10
MIR-130b 239.94 173.32 428.58 82.58 190.41
MIR-132 61.16 32.87 47.40 24.91 80.16
MIR-133a-1,2 1478.76 1913.47 4147.62 746.93 2080.98
MIR-133b 1874.82 1182.64 3505.91 968.99 2782.82
MIR-134 5729.84 2114.71 9802.38 1311.28 7729.64
MIR-135a-1,2 52.20 21.35 295.44 10.34 452.52
MIR-135b-P 448.43 261.97 778.06 145.47 709.39
MIR-136 118.44 132.40 160.18 23.63 102.57
MIR-137* 14.79 17.67 71.14 13.33 101.48
MIR-138-1,2 54.06 36.87 151.82 3.74 131.09
MIR-139 2982.05 731.49 4998.87 450.09 3804.79
MIR-140 1.91 1.63 6.95 0.00 5.36
MIR-141 273.58 110.40 502.94 17.18 285.18
MIR-142-P 4106.24 2751.41 11185.70 1328.93 15395.12
MIR-143 658.22 327.55 1097.02 151.76 822.89
MIR-144 6.87 8.66 14.55 8.02 18.61
MIR-145-P 1110.14 386.56 1250.66 214.01 1282.89
MIR-146 60.02 14.45 106.86 9.64 71.46
MIR-147 326.07 96.59 513.02 51.41 512.53
MIR-148a-P 186.34 48.55 300.14 65.68 315.33
MIR-148b-P 880.30 387.60 1262.86 128.64 1764.13
MIR-149 2158.86 1228.15 4215.90 563.31 2698.16
MIR-150 782.01 408.29 1389.83 178.73 1074.33
MIR-151 371.93 110.58 813.58 27.91 472.92
MIR-152 168.31 256.71 340.49 272.63 507.01
MIR-153-1,2 152.19 147.65 129.60 53.05 96.22
MIR-154 74.29 41.38 260.06 16.33 144.73
MIR-155 80.38 107.04 107.64 4.32 89.60
MIR-181a,c 447.28 620.27 806.30 97.59 409.56
MIR-181b-1,2 139.44 99.19 229.24 27.85 242.17
MIR-182 7325.60 1833.42 3938.31 465.06 3876.78
MIR-183 327.81 96.59 192.64 33.02 210.72
MIR-184 1892.12 905.51 3621.02 671.69 1185.44
MIR-185 6032.23 2332.06 9623.37 1500.70 3822.44
MIR-186-P 2659.76 1744.18 9527.89 550.08 3317.72
MIR-187 181.07 202.73 517.92 197.08 256.41
MIR-188b-P 498.70 166.67 994.36 141.09 752.37
MIR-190 15.83 59.15 14.99 39.90 39.63
MIR-191 151.52 49.75 286.89 6.90 155.01
Continued
234
Table C.1. continued
MIR-192 0.00 0.00 0.00 0.00 0.00
MIR-193 462.00 136.08 738.65 206.70 605.22
MIR-194-1,2 279.89 88.99 548.62 16.74 411.24
MIR-195-P 15927.75 6364.69 33332.36 3295.25 25899.02
MIR-196a-1 453.71 411.79 481.64 189.05 662.24
MIR-196a-2 327.33 46.25 803.54 130.94 282.45
MIR-196b 7154.48 2691.84 3038.97 2540.60 3251.90
MIR-197 17908.72 10930.58 19754.36 4470.89 16845.99
MIR-198 12.34 292.61 13.19 2.04 70.93
MIR-199a-1-P 3457.14 2498.51 3105.85 2122.33 4079.92
MIR-199a-2-P 696.18 3597.22 1302.08 2368.02 900.48
MIR-199b-P 5659.02 2204.29 8779.40 1289.17 5365.19
MIR-200a-P 100103.73 101638.35 144466.94 64438.67 171307.39
MIR-200b 126.85 139.39 404.22 62.70 304.12
MIR-200c 235.17 45.70 539.77 58.61 364.74
MIR-203 151.04 117.60 317.63 40.81 267.75
MIR-204 3233.23 1203.17 6361.16 694.51 6005.70
MIR-205 2749.62 1267.39 2885.66 790.15 4024.49
MIR-206 352.40 140.91 701.21 49.18 666.24
MIR-208 113.87 122.62 252.76 52.96 228.10
MIR-210 15197.87 24887.13 24766.25 23943.21 8658.47
MIR-211 6933.81 3403.82 12057.56 1726.98 8537.68
MIR-212 631.76 240.09 1060.80 153.28 883.01
MIR-213 191.89 179.01 485.61 85.86 200.71
MIR-214 3786.62 2262.78 8176.70 956.18 5818.80
MIR-215 92.32 52.54 131.80 38.23 171.35
MIR-216 601.33 256.10 1134.51 133.74 822.36
MIR-217 12.64 2.04 19.29 5.59 11.87
MIR-218-1,2 118.76 2951.44 207.92 1159.07 143.27
MIR-219-1,2 82.25 366.00 135.39 295.67 218.89
MIR-220 840.95 246.02 647.88 208.23 469.76
MIR-221 671.66 167.92 586.67 156.66 1236.55
MIR-222-P 640.72 239.75 969.30 114.25 581.48
MIR-223 254.31 39.10 290.81 17.41 314.21
MIR-224 482.70 162.84 664.38 149.62 1078.74
MIR-296 593.97 213.61 1582.79 92.71 1016.76
MIR-299-P 834.48 749.58 3344.78 398.50 2253.92
MIR-301 402.60 710.74 691.72 612.24 736.99
MIR-302 87.36 278.52 151.71 45.39 139.47
MIR-302b 384.89 174.11 482.25 68.35 425.28
MIR-302c 405.12 129.41 1129.58 24.63 777.64
Continued
235
Table C.1. continued
MIR-302d 333.91 115.87 837.21 21.62 645.86
MIR-320 495.18 330.76 1074.47 152.23 1046.60
MIR-321 12633.16 7751.71 12660.01 7624.21 38764.26
MIR-323 307.22 231.00 498.40 103.57 684.46
MIR-324-P 964.56 884.51 1556.49 349.54 1590.87
MIR-325 643.14 45.07 141.98 80.62 157.49
MIR-326-P 145.30 45.63 307.32 39.55 192.77
MIR-328 6705.11 1794.17 11160.98 878.38 10478.66
MIR-330 976.01 323.99 1695.54 177.45 1643.84
MIR-331-P 881.57 293.60 1598.51 74.19 1153.36
MIR-335 38.60 14.04 86.72 3.04 35.00
MIR-337 1302.14 526.90 2495.57 368.88 2050.33
MIR-338 1383.21 474.44 1826.35 189.56 1887.33
MIR-339 1522.38 791.77 1388.57 503.36 1472.69
MIR-340 166.89 137.59 587.12 42.34 365.17
MIR-342 594.31 125.11 1781.11 133.77 1409.85
MIR-345 5520.16 1625.70 7090.30 1002.69 8379.26
MIR-346 2615.44 958.33 5360.92 516.74 2845.74
MIR-361 105.57 66.89 124.44 110.76 303.97
MIR-367 4.57 12.52 44.95 1.59 3.31
MIR-368 12.66 4.00 23.24 2.92 11.53
MIR-369 1.68 0.00 0.00 0.00 0.00
MIR-370 487.40 364.35 898.71 168.74 587.33
MIR-371 3061.09 1682.88 4857.15 622.86 3302.94
MIR-372 43.68 3.79 72.51 27.82 64.05
MIR-373 557.55 175.04 1249.87 89.80 597.21
MIR-374-P 0.00 1.03 7.89 0.00 0.00
MIR-375 30.10 32.92 28.42 67.27 58.26
MIR-376 3.78 147.74 2.97 47.57 42.88
MIR-377 120.72 52.02 127.89 56.21 330.70
MIR-378 104.49 11.12 92.28 67.28 242.08
MIR-379 9.64 124.19 3.01 144.09 21.24
MIR-380 22.92 6.37 6.80 3.99 57.08
MIR-381 13.91 1.17 58.61 2.51 78.44
MIR-382 50.42 15.40 14.58 3.62 66.50
MIR-383 69.88 11.88 44.99 28.25 155.36
MIR-384 0.00 0.00 0.00 0.00 0.00
MIR-422b 1054.18 387.48 2299.62 32.64 1401.36
MIR-423 568.32 274.84 3562.62 137.58 846.98
MIR-424 28.82 109.27 123.45 14.68 81.20
MIR-425 89.95 92.16 49.76 38.87 304.93
Continued
236
Table C.1. continued
Breast Cancer
miRNA T47D SKBR3 MDA361 MCF7 MDA231
let-7a-1* 62.25 43.55 158.66 81.18 42.38
let-7a-2-P 688.29 1113.40 1507.16 860.86 563.29
let-7a-3* 111.26 74.66 53.56 51.86 205.56
let-7b* 175.54 113.98 62.44 75.15 176.07
let-7c* 0.00 11.87 12.70 3.22 10.72
let-7d* 273.02 283.48 422.64 319.91 210.57
let-7e* 8.64 8.14 17.71 7.94 9.09
let-7f-1* 479.87 214.50 441.00 439.57 279.89
let-7f-2* 19.25 18.62 30.97 18.75 12.32
let-7g* 84.74 80.38 152.01 39.30 103.62
let-7i* 121.17 34.10 42.70 105.70 248.82
MIR-001-1 111.86 441.72 560.64 136.05 82.99
MIR-001-2 271.90 377.84 700.50 374.80 235.78
MIR-007-1 76.16 122.37 175.51 243.58 129.69
MIR-007-2 60.77 66.59 168.69 104.48 97.31
MIR-007-3 40.55 32.19 43.46 39.24 32.80
MIR-009-1-P 2837.00 6147.47 6808.78 2913.26 3389.38
MIR-009-2-P 2474.60 3521.04 5729.07 2588.92 2095.63
MIR-009-3-P 62.92 59.04 72.35 78.77 39.65
MIR-010a,b 6.32 3.45 3.26 2.00 94.73
MIR-015a 287.29 332.38 611.60 219.33 150.22
MIR-015b 1226.15 983.08 1532.48 456.28 413.33
MIR-016-1 786.46 360.43 400.51 519.05 920.46
MIR-016-2 942.96 827.13 683.45 543.06 575.55
MIR-017-P 730.07 1136.57 1259.00 701.41 603.79
MIR-018 912.46 2437.49 1159.92 443.18 1616.64
MIR-019a-P 243.80 797.03 696.68 150.88 382.36
MIR-019b-1-P 1147.48 684.13 702.13 1380.25 1521.93
MIR-019b-2-P 3.83 4.76 7.75 2.44 5.49
MIR-020 2.82 5.46 7.34 9.83 2.22
MIR-021 4449.65 1695.60 3734.70 54580.57 23376.82
MIR-022-P 310.62 594.88 999.26 605.58 1611.30
MIR-023a 484.17 856.21 1263.72 648.93 533.03
MIR-023b-P 1156.68 1829.98 2632.32 2217.37 1062.31
MIR-024-1,2 6014.17 6789.07 4590.01 10811.48 5329.28
MIR-025 444.13 1221.73 707.49 418.93 356.35
MIR-026a-1,2 298.20 200.80 441.08 149.64 89.68
MIR-026b 1499.59 1935.09 2380.76 1562.26 530.44
MIR-027a, b 916.93 1477.60 1834.31 1120.67 940.45
Continued
237
Table C.1. continued
MIR-028 56.31 57.74 72.81 38.91 60.11
MIR-029a,c 108.76 310.95 317.41 118.38 909.67
MIR-029b-1,2 11.38 77.04 103.42 11.28 27.64
MIR-030a,e 482.77 381.29 854.43 416.18 342.82
MIR-030b 465.52 900.25 1762.20 1069.91 1149.89
MIR-030c-1,2 719.85 851.89 959.04 684.72 454.47
MIR-030d 80.78 17.98 413.93 112.75 15.74
MIR-031-P 4357.22 5886.28 7941.88 4975.91 6153.68
MIR-032 144.78 63.81 360.54 223.48 97.75
MIR-033 23.94 15.67 26.07 17.58 10.55
MIR-034a 402.02 724.91 828.41 411.77 366.20
MIR-034b 87.83 61.84 114.55 74.69 77.56
MIR-034c 0.00 0.00 0.00 0.00 0.00
MIR-092-1 43.91 37.47 42.58 2.38 10.81
MIR-092-2-P 199.46 285.98 374.90 237.64 102.99
MIR-093 527.99 700.97 1078.20 404.09 397.40
MIR-095 6.57 10.17 32.50 15.51 16.14
MIR-096-P 8705.83 19556.09 16927.21 12463.90 15769.94
MIR-098 3923.73 3912.68 5167.08 4590.02 2513.38
MIR-099a 13.05 41.01 56.81 58.62 26.45
MIR-099b 433.84 718.37 644.30 880.44 562.50
MIR-100 34.23 61.54 31.70 9.39 4910.96
MIR-101-1 57.19 53.99 91.15 17.70 7.35
MIR-101-2 431.29 496.55 444.50 757.69 705.47
MIR-103-1,2 2824.43 3735.04 4344.53 4052.00 3637.89
MIR-105-1,2 182.63 248.15 494.46 177.27 51.29
MIR-106a-P 39.99 76.79 132.13 48.68 34.83
MIR-106b 847.46 2181.43 544.63 1513.56 2151.66
MIR-107 3872.58 4127.91 2194.33 4054.20 5617.71
MIR-108 377.90 431.93 641.51 222.86 247.58
MIR-122a 23.34 48.77 109.86 83.45 70.56
MIR-124a-1,2,3 44.36 147.50 170.64 88.34 64.48
MIR-125a 1029.68 1861.53 4424.16 1454.55 695.39
MIR-125b-1 20.24 42.81 35.40 10.71 299.73
MIR-125b-2 112.08 187.57 266.78 136.81 152.50
MIR-126 43.94 66.09 78.80 100.39 40.93
MIR-127 5745.33 5878.40 5840.05 5391.94 4800.48
MIR-128a 333.52 1081.04 1726.56 419.10 327.99
MIR-128b 113.44 175.13 210.29 96.97 103.98
MIR-129-2 4823.24 6984.59 7619.57 6112.24 4036.01
MIR-130a-P 4924.21 6820.42 10705.49 5561.44 4933.34
Continued
238
Table C.1. continued
MIR-130b 68.69 175.56 286.05 44.94 39.88
MIR-132 7.86 10.36 82.39 42.26 17.01
MIR-133a-1,2 671.92 674.48 1000.23 636.45 657.88
MIR-133b 986.75 971.33 1173.69 887.52 915.55
MIR-134 2147.20 2854.69 3493.59 2083.45 3436.23
MIR-135a-1,2 77.93 53.95 52.20 68.79 57.72
MIR-135b-P 142.27 306.25 455.58 67.81 410.20
MIR-136 23.42 36.00 56.89 29.24 42.38
MIR-137* 20.93 32.20 31.22 54.54 19.87
MIR-138-1,2 48.47 48.96 66.88 27.36 24.62
MIR-139 1242.27 1423.97 1564.71 1115.19 810.34
MIR-140 0.00 0.83 3.33 0.99 1.02
MIR-141 61.85 175.44 135.60 109.94 82.61
MIR-142-P 834.07 1803.41 2639.43 1164.34 1240.16
MIR-143 147.35 443.79 502.10 234.25 164.74
MIR-144 6.16 9.64 8.24 65.90 6.29
MIR-145-P 147.76 589.08 408.63 353.07 371.05
MIR-146 8.43 13.66 27.70 16.89 17.70
MIR-147 99.63 150.80 332.58 88.94 95.80
MIR-148a-P 74.31 69.59 60.83 75.62 82.62
MIR-148b-P 857.16 586.37 1727.98 397.96 414.32
MIR-149 1387.37 1312.37 1989.93 1344.34 1761.82
MIR-150 503.48 478.36 548.66 382.17 477.60
MIR-151 137.06 174.13 353.87 255.64 129.77
MIR-152 48.91 73.20 106.41 137.98 171.43
MIR-153-1,2 31.71 37.01 57.91 40.75 86.26
MIR-154 4.63 30.12 39.11 11.69 64.67
MIR-155 10.48 41.57 150.38 105.92 382.40
MIR-181a,c 526.25 320.93 352.45 164.21 894.54
MIR-181b-1,2 90.07 185.35 226.58 101.45 194.93
MIR-182 4001.65 4445.20 1426.94 8261.33 8280.74
MIR-183 148.97 191.99 98.05 291.27 390.85
MIR-184 1411.99 1536.55 2368.26 1542.60 1152.12
MIR-185 2970.97 3586.65 3673.96 3417.54 2484.17
MIR-186-P 1776.40 3199.59 4034.67 2033.39 1559.13
MIR-187 58.54 146.67 173.64 125.80 61.46
MIR-188b-P 131.00 171.89 358.77 169.89 108.23
MIR-190 12.21 27.22 28.14 0.95 2.63
MIR-191 43.94 69.87 92.95 46.02 12.11
MIR-192 0.00 0.00 0.00 0.00 0.00
MIR-193 347.41 306.98 320.03 203.38 206.30
Continued
239
Table C.1. continued
MIR-194-1,2 36.90 108.57 56.38 96.72 41.80
MIR-195-P 4392.29 8430.40 8891.78 5641.60 8186.25
MIR-196a-1 121.91 210.47 429.53 334.39 143.51
MIR-196a-2 377.53 316.74 617.67 162.95 249.69
MIR-196b 222.43 863.03 1063.27 445.86 735.45
MIR-197 7025.10 24608.73 13504.50 3916.36 1973.59
MIR-198 30.19 7.97 9.39 1.77 2.97
MIR-199a-1-P 2006.88 2718.44 3351.06 2476.13 1998.50
MIR-199a-2-P 331.62 989.45 1328.12 360.36 244.54
MIR-199b-P 2375.35 3000.15 3298.06 3261.80 2230.47
MIR-200a-P 57526.35 32110.60 68020.47 46917.79 66490.12
MIR-200b 93.33 58.59 234.11 174.94 34.20
MIR-200c 88.20 136.54 222.29 145.93 57.19
MIR-203 88.75 61.64 50.97 39.79 7.83
MIR-204 803.02 1462.54 2400.18 379.38 1870.90
MIR-205 273.21 77.85 234.10 28.61 22.36
MIR-206 11.87 120.80 339.45 115.18 31.33
MIR-208 61.45 94.89 84.41 61.91 39.13
MIR-210 1251.49 10642.91 8897.04 13227.19 15161.91
MIR-211 3511.02 3749.33 6996.25 3879.47 3316.68
MIR-212 124.33 206.66 323.50 259.13 437.06
MIR-213 270.56 235.05 201.22 264.04 725.56
MIR-214 1818.16 2440.67 3668.78 1916.36 1540.07
MIR-215 174.40 178.94 249.84 87.29 124.09
MIR-216 192.32 280.14 408.72 228.03 181.29
MIR-217 7.41 26.01 17.80 7.90 2.19
MIR-218-1,2 28.19 593.60 709.95 94.89 20.51
MIR-219-1,2 84.76 132.03 130.01 49.52 148.28
MIR-220 149.46 258.83 299.02 289.09 680.37
MIR-221 35.67 37.02 38.03 37.22 243.54
MIR-222-P 56.47 110.29 160.25 133.10 99.38
MIR-223 54.56 135.34 217.84 85.06 53.51
MIR-224 181.41 286.00 281.54 168.92 142.44
MIR-296 251.92 89.98 538.08 334.28 253.02
MIR-299-P 585.45 920.19 1897.78 2717.80 569.88
MIR-301 788.21 286.71 374.65 1137.02 612.53
MIR-302 89.48 94.55 277.30 120.71 23.64
MIR-302b 165.52 346.73 306.09 226.29 137.81
MIR-302c 115.90 191.66 321.50 161.90 131.02
MIR-302d 128.09 220.19 275.97 84.05 86.58
MIR-320 409.76 438.32 750.84 393.78 488.45
Continued
240
Table C.1. continued
MIR-321 2457.71 7312.89 15327.99 68262.76 11380.68
MIR-323 619.72 592.20 526.24 169.92 96.00
MIR-324-P 571.93 614.68 926.84 794.07 519.32
MIR-325 42.11 40.02 37.14 105.82 17.09
MIR-326-P 34.98 49.61 101.96 83.70 32.27
MIR-328 1339.08 2747.11 4416.14 1934.46 1887.74
MIR-330 272.23 342.89 674.43 464.94 357.97
MIR-331-P 201.32 583.44 987.66 406.33 365.48
MIR-335 8672.16 15.48 23.83 16.20 18.10
MIR-337 460.97 774.96 697.24 75.04 530.19
MIR-338 625.99 872.31 1090.44 702.12 634.21
MIR-339 987.15 965.65 943.34 978.02 764.80
MIR-340 602.43 212.03 956.98 864.57 1010.18
MIR-342 252.31 317.36 403.08 450.77 251.75
MIR-345 1529.57 2797.94 3450.28 1806.99 523.98
MIR-346 1247.74 1507.08 1152.95 2164.04 1719.51
MIR-361 165.11 82.17 50.93 198.82 96.81
MIR-367 8.28 24.84 17.59 40.50 23.38
MIR-368 0.00 0.94 1.99 6.15 3.12
MIR-369 0.00 2.16 0.00 0.00 0.00
MIR-370 167.34 193.30 369.51 198.67 144.19
MIR-371 841.11 1591.51 1663.46 1318.69 1749.81
MIR-372 5.36 7.48 38.52 19.75 4.56
MIR-373 194.64 286.49 353.84 228.32 151.31
MIR-374-P 24.94 5.49 6.11 1.57 0.00
MIR-375 59.32 25.95 101.19 72.24 17.45
MIR-376 4.13 2.51 2.07 15.51 7.56
MIR-377 109.56 11.34 30.87 347.19 71.63
MIR-378 37.30 56.46 54.87 234.68 63.52
MIR-379 1.44 7.31 6.37 16.52 2.84
MIR-380 11.86 4.24 34.21 4.58 4.52
MIR-381 15.27 6.16 36.54 27.24 8.73
MIR-382 9.67 7.87 9.30 14.27 27.48
MIR-383 66.51 20.24 28.99 130.99 84.30
MIR-384 0.00 0.00 0.00 0.00 0.00
MIR-422b 658.88 831.07 1622.54 853.33 473.31
MIR-423 232.65 350.31 270.22 225.82 200.45
MIR-424 12.14 89.07 56.92 126.24 206.92
MIR-425 217.74 155.79 565.54 89.11 233.39
Colorectal Cancer
Continued
241
Table C.1. continued
miRNA HCT8 HCT116 HT29 SW620 HCT15 HeLa
let-7a-1* 858.6328 26.56572 103.2427 118.4805 183.6582 25.74712
let-7a-2-P 396.5826 1448.423 904.466 944.8584 1740.836 1857.928
let-7a-3* 779.3264 33.83821 460.5481 191.8136 388.4083 71.64201
let-7b* 666.7015 140.1044 430.6308 177.4954 416.9417 150.0497
let-7c* 2.139169 0 0 1.966706 0 0
let-7d* 1652.745 319.0011 623.4727 643.5575 287.5726 111.6563
let-7e* 17.60674 0 27.02524 0 16.84116 0
let-7f-1* 1983.959 480.2421 847.6834 737.6523 495.6824 132.7326
let-7f-2* 67.00685 24.49063 38.95639 21.29969 18.34627 9.176052
let-7g* 625.3398 143.5549 63.57787 256.2802 163.6351 26.97658
let-7i* 225.3582 87.03372 51.60207 180.7823 160.3876 228.2109
MIR-001-1 29.96919 125.0151 15.76709 218.114 331.3349 320.1529
MIR-001-2 452.1449 261.2442 265.1213 306.5967 1177.074 337.7787
MIR-007-1 88.78428 148.2617 158.5793 173.1882 262.1453 102.6599
MIR-007-2 26.99634 49.14339 141.9696 137.9683 146.9345 207.0472
MIR-007-3 2.197971 23.89761 59.15871 4482.319 41.89004 149.18
MIR-009-1-P 1451.901 6319.989 4207.668 2610.739 4601.714 1455.045
MIR-009-2-P 913.5585 2653.155 2516.776 3651.288 7360.267 7251.747
MIR-009-3-P 5.910899 74.97863 62.53537 60.41976 67.56843 96.99898
MIR-010a,b 619.1 86.19631 266.6186 78.49641 1901.472 56.19538
MIR-015a 145.9512 378.2724 333.8203 495.4617 776.2445 502.9424
MIR-015b 386.8024 931.5294 706.1382 900.2918 1107.319 502.2059
MIR-016-1 660.0388 876.3006 702.3629 1205.397 2270.934 276.4983
MIR-016-2 300.8195 738.2241 727.1829 613.9356 858.8586 1157.982
MIR-017-P 424.6995 1359.089 1377.29 3260.714 2047.729 900.4742
MIR-018 2081.512 2616.175 6256.62 12520.61 8806.856 1728.682
MIR-019a-P 416.7196 731.731 1286.29 4039.599 3258.111 1022.288
MIR-019b-1-P 864.9197 859.0292 1785.739 1182.591 1936.753 579.6239
MIR-019b-2-P 0 4.336451 10.56738 0 5.713239 4.062523
MIR-020 7.505857 14.61272 28.04179 32.71384 11.72935 1.32159
MIR-021 50874.99 58938.4 17778.59 14369.48 4720.269 6712.824
MIR-022-P 1909.665 712.7892 1911.439 2520.227 757.4464 733.3245
MIR-023a 240.2744 592.8306 716.3597 779.2062 1407.155 1201.024
MIR-023b-P 675.0464 1962.236 1303.544 1594.949 2894.791 360.3628
MIR-024-1,2 3769.404 3275.857 7780.918 10350.49 10848.19 6529.597
MIR-025 418.9766 438.5579 1004.11 1740.284 1624.791 506.4707
MIR-026a-1,2 246.9806 145.2754 233.6056 304.7698 298.4189 318.475
MIR-026b 672.3596 1600.749 1511.269 1402.817 2249.309 1154.567
MIR-027a, b 603.8183 1323.765 1324.007 1223.712 1941.031 1531.911
MIR-028 18.96326 31.46524 33.42726 47.57557 81.76565 42.16035
Continued
242
Table C.1. continued
MIR-029a,c 175.5249 923.1972 241.7734 748.8847 967.5754 109.7668
MIR-029b-1,2 3.131238 25.64242 73.733 13.69045 26.82112 21.42734
MIR-030a,e 131.2142 186.5888 241.3156 259.7985 622.7983 360.9059
MIR-030b 293.2278 1113.478 1116.101 991.1852 1655.255 941.8097
MIR-030c-1,2 425.1509 691.1524 853.9301 679.8306 1435.298 729.6663
MIR-030d 50.05628 95.89363 172.0392 103.4067 182.5863 29.27537
MIR-031-P 3636.022 9515.312 6730.927 4758.911 11835.72 6526.764
MIR-032 175.8589 116.0979 187.4735 137.3989 555.828 131.0234
MIR-033 6.380136 7.845837 13.69873 26.96014 21.39319 32.09109
MIR-034a 114.7969 404.5851 689.6787 508.8551 616.5719 509.5557
MIR-034b 43.90841 151.7012 76.38421 114.3459 151.8128 52.63946
MIR-034c 0 0 0 0 0 0
MIR-092-1 52.24811 52.30043 95.98827 156.6745 251.6343 47.65748
MIR-092-2-P 89.67869 311.23 277.6539 203.2726 379.0785 297.8797
MIR-093 292.1088 723.8734 827.0072 939.9623 986.3213 420.677
MIR-095 47.0068 26.42547 4.353642 19.85175 51.82277 14.76487
MIR-096-P 7814.41 23747.99 17050.31 14472.33 37251.3 14151.99
MIR-098 2120.708 2253.724 3885.721 9781.361 13142.37 3682.978
MIR-099a 6.977367 25.569 20.92022 26.31396 136.5722 97.52835
MIR-099b 436.9488 243.2771 491.9417 230.9324 1442.155 1442.525
MIR-100 7.230838 8366.922 16.65482 405.3392 54.78532 1002.784
MIR-101-1 26.54289 7.940138 10.34086 61.59516 44.3979 21.81055
MIR-101-2 223.2018 361.7411 537.9242 1202.656 655.1078 247.3087
MIR-103-1,2 1947.736 3288.189 3458.059 5432.269 8465.019 3404.049
MIR-105-1,2 107.2489 193.9397 217.3292 202.5615 363.8384 343.6853
MIR-106a-P 17.82535 77.62886 71.00889 83.51111 116.1518 73.04081
MIR-106b 1194.63 1314.696 3225.651 4127.674 3566.511 607.9477
MIR-107 2766.868 3405.163 4607.7 8226.097 12181.71 2083.073
MIR-108 94.60239 278.2872 322.534 190.7989 512.1909 3.082367
MIR-122a 7.459651 36.69896 29.73935 43.41953 111.0434 155.046
MIR-124a-1,2,3 13.92713 75.44121 63.41554 100.1347 167.9732 65.45739
MIR-125a 312.2859 1121.213 730.2028 743.4873 1311.396 756.9644
MIR-125b-1 4.426267 1165.244 32.6417 47.81384 44.05668 168.745
MIR-125b-2 29.39134 197.8012 124.2387 78.89522 83.40107 123.868
MIR-126 45.1213 131.4173 66.74584 72.37189 29.29237 428.2762
MIR-127 3789.418 3821.264 5811.132 4529.653 3397.028 4810.069
MIR-128a 409.2436 617.281 398.6055 323.1376 1078.223 540.5957
MIR-128b 24.79734 67.95487 81.44437 106.2937 81.75552 132.3815
MIR-129-2 3252.573 7358.586 6573.032 5799.161 9972.255 8423.595
MIR-130a-P 2527.761 7547.183 6471.418 6467.01 8418.279 6466.665
MIR-130b 60.07852 20.81728 45.86715 110.1567 154.7273 40.43121
Continued
243
Table C.1. continued
MIR-132 3.392268 10.54607 30.60755 43.08519 43.57966 39.57838
MIR-133a-1,2 593.6712 664.8818 671.4538 449.8052 1335.077 551.3551
MIR-133b 414.7147 1359.772 1029.132 1266.517 512.5088 1947.167
MIR-134 1134.351 1901.47 2974.258 2484.47 426.9678 3013.016
MIR-135a-1,2 108.5499 35.63658 59.46605 108.3684 399.6547 94.2825
MIR-135b-P 213.8267 495.0453 333.3833 292.3017 162.6791 348.2613
MIR-136 16.42341 32.59869 23.23807 35.61811 67.26193 81.64402
MIR-137* 6.706809 17.59405 22.47996 39.4592 45.94706 3.584687
MIR-138-1,2 7.840621 33.79533 20.07316 19.30726 60.12293 54.64548
MIR-139 676.9869 1964.388 1506.958 1148.339 1990.836 1455.485
MIR-140 0.99355 0.77326 0 0 2.08558 0
MIR-141 96.90152 75.62146 101.0013 51.94906 237.3693 153.924
MIR-142-P 742.1889 2180.873 1944.425 93.08532 2444.312 2174.696
MIR-143 116.873 242.5868 208.6492 144.0841 514.7473 514.8569
MIR-144 4.672227 0.628385 2.17791 3.061594 5.872603 0
MIR-145-P 161.9171 523.5928 388.4528 313.7008 351.346 243.1834
MIR-146 4.326121 182.8769 15.50638 29.38146 11.5396 31.6192
MIR-147 52.03633 190.5483 161.8781 343.2752 332.4611 200.0979
MIR-148a-P 23.38892 104.532 63.98417 51.15979 80.02053 15.87187
MIR-148b-P 191.9018 503.0921 378.48 466.9279 1703.11 682.4618
MIR-149 273.87 263.1702 1473.368 1224.909 2369.59 1814.888
MIR-150 225.8272 463.4491 518.1132 495.5266 795.23 928.579
MIR-151 48.46468 221.9175 242.1159 248.1392 277.7097 394.1336
MIR-152 77.45324 69.03609 43.70144 75.43123 196.9967 114.6715
MIR-153-1,2 13.86209 103.8692 22.36573 265.4505 91.39711 25.4118
MIR-154 3.869608 22.38215 33.1496 24.34255 38.98702 38.05686
MIR-155 12.42286 55.04169 77.95959 15.36145 259.746 134.442
MIR-181a,c 143.8996 332.8634 561.3267 1189.489 427.5878 453.3771
MIR-181b-1,2 32.94578 110.9696 69.27151 65.49388 191.7068 164.9875
MIR-182 3471.177 11760.68 7875.101 14851.87 11546.05 1052.715
MIR-183 167.8081 493.7559 211.6685 452.9504 314.8324 55.94216
MIR-184 482.8474 1217.5 1302.477 1503.389 2430.265 1696.498
MIR-185 1655.257 3543.113 3587.202 2314.415 3844.361 2252.288
MIR-186-P 1534.839 2294.337 3083.847 3085.927 9961.895 2040.209
MIR-187 48.8742 172.2086 94.01175 85.77991 77.46041 150.1551
MIR-188b-P 85.91987 224.7902 168.7507 185.2942 242.2323 208.9167
MIR-190 0 1.627128 1.100183 16.3053 12.03259 20.90265
MIR-191 19.70146 25.8896 32.86388 47.7036 53.94927 47.63331
MIR-192 0 1074.752 0 523.274 610.6581 519.2557
MIR-193 94.17563 198.2033 156.1941 46.82278 246.4542 334.6753
MIR-194-1,2 28.68354 77.98645 111.8687 147.7562 117.6403 54.10766
Continued
244
Table C.1. continued
MIR-195-P 3503.953 13905.82 8484.665 7887.77 15530.54 7771.25
MIR-196a-1 1109.454 313.8951 1008.097 287.2314 926.9358 494.4576
MIR-196a-2 89.39692 101.5213 148.4484 200.5871 369.093 65.10065
MIR-196b 430.8136 14753.53 24194.42 11119.59 10390.38 3911.31
MIR-197 3171.504 12127.31 5059.421 5976.362 14323.88 15025.78
MIR-198 0.810397 41.22313 3.835345 3.398553 6.038527 9.890589
MIR-199a-1-P 858.5674 1301.236 2448.376 3025.907 1992.933 1805.783
MIR-199a-2-P 157.97 320.2873 260.9154 592.8856 513.4992 195.426
MIR-199b-P 1029.394 3249.48 1077.528 2604.771 4301.675 6835.134
MIR-200a-P 36539.68 92229.37 75610.08 49235.55 125174 55241.49
MIR-200b 163.5854 62.35029 760.2065 172.5957 204.7594 108.5994
MIR-200c 108.2049 35.02744 137.3241 76.14315 202.6843 239.9847
MIR-203 94.28691 14.61154 60.7142 73.73222 163.786 77.26294
MIR-204 840.4258 1995.016 1954.807 1305.777 2403.835 1853.281
MIR-205 5.302058 6.012355 21.23884 38.19757 35.29727 20.44176
MIR-206 31.23536 171.1748 226.168 184.5706 427.2351 142.4579
MIR-208 13.67356 85.60709 68.01402 66.61015 122.5556 144.5863
MIR-210 4693.817 29509.37 8259.725 6299.346 29003.47 63.51755
MIR-211 1760.765 3780.557 3280.637 3808.365 6854.794 3392.772
MIR-212 238.8805 492.1094 246.0567 881.8622 1346.46 404.9515
MIR-213 35.85119 722.4231 436.9843 360.1484 358.7738 142.3082
MIR-214 1040.015 1724.102 3260.109 1721.53 2832.766 1827.1
MIR-215 53.17391 103.3322 76.92807 59.78262 155.5646 53.13787
MIR-216 112.0725 277.7547 306.5443 259.5179 359.4469 351.6544
MIR-217 15.26014 6.470761 3.532712 2.278755 19.34929 3.729676
MIR-218-1,2 7.637745 66.02772 30.49248 39.07788 60.19512 72.05416
MIR-219-1,2 83.49827 42.5829 47.46584 88.41967 198.1093 71.7328
MIR-220 123.3407 245.8683 202.4307 179.7148 509.197 282.5261
MIR-221 107.3138 794.0723 273.5634 397.8852 343.9514 127.902
MIR-222-P 31.33221 3017.878 166.1777 120.7482 339.7038 231.9186
MIR-223 27.62982 27.98896 64.82215 64.75493 101.7956 3.497619
MIR-224 88.74434 129.1676 272.4346 250.7757 263.6765 855.595
MIR-296 95.04047 354.2562 342.4293 228.2298 361.4993 768.5301
MIR-299-P 368.4251 1967.997 682.9783 860.2205 1398.926 1444.011
MIR-301 252.6409 267.3405 234.8289 930.1332 1532.321 213.291
MIR-302 18.28203 63.12679 81.33678 56.05573 86.99968 27.34738
MIR-302b 83.838 98.12517 143.8903 169.9355 305.597 318.0692
MIR-302c 50.42963 172.1014 169.3356 135.0086 302.1697 0
MIR-302d 39.031 163.2747 178.0246 131.7586 245.5951 183.7419
MIR-320 489.3002 412.7287 502.0868 609.2567 1476.951 532.4651
MIR-321 5699.42 20846.95 16162.7 5800.888 20094.89 5317.977
Continued
245
Table C.1. continued
MIR-323 65.27868 129.7469 119.1797 137.3871 411.4272 382.2681
MIR-324-P 537.6329 619.6461 792.0435 839.5042 1588.497 690.3973
MIR-325 68.18792 42.85667 70.74943 313.2638 114.5458 42.42922
MIR-326-P 6.368802 67.8049 48.52222 43.86994 83.51881 104.3394
MIR-328 1001.711 3079.63 2991.854 2181.811 4949.716 4486.09
MIR-330 268.1315 545.9928 374.8421 402.7715 576.319 511.4956
MIR-331-P 268.4213 368.9336 426.6046 389.2076 912.9941 30.81881
MIR-335 11.53739 34.5304 11.36867 4.327626 8.429286 5.887744
MIR-337 410.4399 567.2167 520.4553 646.2871 1048.427 769.6332
MIR-338 202.4377 891.3485 467.7347 693.0709 1121.582 278.0501
MIR-339 553.6613 1137.904 938.9432 678.1472 1517.897 1570.045
MIR-340 506.743 535.116 406.8421 471.7936 1320.466 231.1919
MIR-342 97.63136 323.29 242.2573 202.1271 402.1698 856.0626
MIR-345 241.1075 1984.368 2432.859 2182.408 3279.497 561.8711
MIR-346 977.9059 2267.955 1449.065 1385.61 1998.735 2077.889
MIR-361 41.62921 156.2936 110.8631 144.5701 166.8953 129.6966
MIR-367 42.87979 21.25949 31.42357 21.77565 96.02627 4.851067
MIR-368 0 7.475348 2.509002 9.705547 27.33484 1.082579
MIR-369 13.40863 0 0 0 0 0
MIR-370 49.93002 318.7627 132.4245 141.267 356.5916 364.7185
MIR-371 267.9453 3764.923 1293.84 961.8371 1768.312 20.88434
MIR-372 4.040082 7.464502 26.28481 0 13.19192 15.65218
MIR-373 58.48832 264.0393 186.106 260.127 391.8836 213.7537
MIR-374-P 0 31.19252 0 13.96407 115.2487 89.98624
MIR-375 89.87842 44.47787 90.58668 206.0609 224.8806 11.34272
MIR-376 0 16.02958 12.74379 6.915397 17.19225 16.01698
MIR-377 21.17526 179.9386 201.2492 226.4024 443.5638 394.4266
MIR-378 36.70699 139.4146 201.9541 220.9498 409.1737 112.3007
MIR-379 0 7.045688 3.157919 3.566075 3.495784 54.92527
MIR-380 0 18.06349 12.7084 43.95977 37.94854 12.48013
MIR-381 2.0587 16.60751 47.25099 34.17626 37.03083 75.91218
MIR-382 3.435885 35.04295 43.54007 83.76556 69.52062 41.85704
MIR-383 10.71362 207.8169 173.9212 119.2419 256.8142 147.2181
MIR-384 0 0 0 0 0 1.575494
MIR-422b 296.4645 707.789 698.0748 765.0957 1151.567 820.1639
MIR-423 92.93823 356.5244 194.9094 158.1259 301.6657 224.4276
MIR-424 16.90948 54.37745 24.08734 286.3969 34.78159 47.78524
MIR-425 1857.477 498.9913 221.6507 396.941 400.8351 23.93992
Hematological Cancer
miRNA RAMOS HL60 K562 JURCAT DAUDI 293T
Continued
246
Table C.1. continued
let-7a-1* 166.6366 13.8676 17.14962 45.91204 49.82124 13.92584
let-7a-2-P 3753.81 746.0296 2851.498 2891.934 3460.508 360.4258
let-7a-3* 0 19.68504 0 21.09172 9.386879 5.125911
let-7b* 18.98209 66.10125 10.16696 13.42023 22.75995 23.46274
let-7c* 68.968 1.539028 0 0 29.12717 0
let-7d* 1313.732 294.2404 321.4057 307.9057 540.0203 114.0851
let-7e* 0 0 10.35786 0 0 0
let-7f-1* 333.5827 47.52613 77.08861 49.36752 174.2227 116.2856
let-7f-2* 81.47435 19.7481 27.74237 57.10392 45.57301 6.957312
let-7g* 963.3425 131.7437 157.3901 313.1559 299.5628 37.97679
let-7i* 154.7413 63.66212 27.74033 45.91237 137.0321 41.47092
MIR-001-1 348.7775 527.14 653.3058 101.7155 519.1936 151.9754
MIR-001-2 648.7846 432.0157 674.5672 290.1393 403.8103 707.4584
MIR-007-1 353.9373 241.9987 182.987 213.5199 241.3748 34.75693
MIR-007-2 189.9284 192.6722 285.8939 284.9488 244.211 91.25417
MIR-007-3 39.40603 57.94656 58.30487 88.11923 76.38345 191.5245
MIR-009-1-P 5285.905 342.5796 6279.909 9198.968 8508.782 3656.139
MIR-009-2-P 6173.907 670.4272 7942.355 8483.259 7111.381 1877.768
MIR-009-3-P 114.6106 11.12941 139.3301 194.4032 233.4594 63.45952
MIR-010a,b 25.52333 4.363303 4.33287 13.69781 2.646115 68.55621
MIR-015a 1274.957 364.2969 480.3505 814.0665 1220.395 226.9485
MIR-015b 2248.663 401.7331 670.3534 899.7338 2042.665 929.5878
MIR-016-1 2343.225 524.5504 326.0847 1775.581 1923.831 504.4688
MIR-016-2 1023.092 1290.183 920.9948 915.8345 1449.551 801.2603
MIR-017-P 6702.649 1519.419 3921.64 3488.064 3969.541 1486.773
MIR-018 26948.86 3956.59 58445.24 9301.178 18844.29 6476.756
MIR-019a-P 11109.49 2408.492 10702.01 3249.181 6637.693 3294.422
MIR-019b-1-P 3763.131 1137.872 1176.243 1445.265 1844.2 339.4511
MIR-019b-2-P 11.29745 0 2.90275 12.76187 17.86829 8.503979
MIR-020 73.75672 12.36713 27.9209 18.37304 53.46666 10.94048
MIR-021 1123.287 429.1152 3577.846 762.0187 2002.423 287.9245
MIR-022-P 584.323 657.0663 716.1434 1024.528 1125.224 481.8155
MIR-023a 1146.567 875.5624 104.4223 1127.198 1842.966 421.3858
MIR-023b-P 2697.446 2557.975 2807.599 2927.525 2907.672 769.3893
MIR-024-1,2 1817.421 2596.787 2183.636 1936.612 1583.455 1706.141
MIR-025 2925.217 862.43 963.5741 1173.715 1175.759 502.8365
MIR-026a-1,2 186.3581 419.2091 485.9575 99.1406 563.6515 174.1412
MIR-026b 2473.301 2665.344 2864.806 3211.984 4327.093 429.3476
MIR-027a, b 2284.84 1969.356 593.0105 2948.62 3674.049 879.3945
MIR-028 89.40984 74.92992 21.50215 81.95749 154.7436 20.34191
MIR-029a,c 561.8704 97.14448 13.54629 351.6844 552.4925 44.04115
Continued
247
Table C.1. continued
MIR-029b-1,2 62.88458 0.638325 74.57517 94.75991 249.1745 32.36581
MIR-030a,e 457.7892 558.6207 592.1317 429.6142 651.5329 154.418
MIR-030b 1676.761 866.2407 1922.159 2108.047 2645.966 733.9129
MIR-030c-1,2 1519.404 363.8571 1509.466 1442.812 2416.158 583.2534
MIR-030d 107.5944 69.02615 37.48549 73.18422 172.9553 87.41096
MIR-031-P 12019.31 1205.848 10478.9 11902.38 11238.99 4207.385
MIR-032 269.4431 124.2691 277.1414 259.4543 365.3757 92.12944
MIR-033 32.96648 11.24012 33.89762 32.09194 74.98626 8.217943
MIR-034a 1500.261 1085.274 1259.33 842.2176 1495.214 286.3861
MIR-034b 173.706 189.3395 231.1241 266.7051 248.9697 82.72159
MIR-034c 0 0 0 0 0 0
MIR-092-1 253.5061 58.98973 807.4232 171.0063 141.5984 117.4945
MIR-092-2-P 400.2367 612.5352 576.7292 775.0618 744.3792 81.69878
MIR-093 1526.799 508.0304 828.0654 1383.011 1220.094 328.1417
MIR-095 9.206388 12.0491 4.199426 7.690361 16.45554 19.71498
MIR-096-P 29596.91 21364.58 18939.54 15973.93 24151.32 10816.44
MIR-098 13177.82 6898.605 5111.825 7710.336 8417.604 1710.517
MIR-099a 33.02749 4.55678 52.15852 48.61796 32.51622 15.72285
MIR-099b 4169.225 459.3873 658.2106 193.2048 196.7277 990.7318
MIR-100 76.48112 89.44438 112.3623 53.02756 93.01511 17.41665
MIR-101-1 95.65256 88.42841 99.6927 75.03961 157.409 30.03772
MIR-101-2 1616.706 623.3177 1041.445 1067.626 862.1386 107.1349
MIR-103-1,2 10752.85 4888.763 5039.741 7216.911 5533.608 2408.706
MIR-105-1,2 593.0519 528.6808 487.0151 444.5546 343.2208 181.3683
MIR-106a-P 118.4912 9.463457 231.5252 215.0468 216.9674 42.91796
MIR-106b 12293.47 989.756 3279.127 2596.978 3434.494 765.6042
MIR-107 14540.57 3397.628 3983.135 7036.947 8073.282 2306.637
MIR-108 443.2815 534.8737 729.2897 163.9282 894.7754 522.8028
MIR-122a 86.95339 111.8566 209.2704 227.3072 176.4073 61.48922
MIR-124a-1,2,3 259.0413 269.9636 40.506 273.0116 264.5819 187.4368
MIR-125a 642.1005 168.9118 755.2254 857.7192 997.5324 408.3563
MIR-125b-1 56.06253 1.072293 77.62582 86.82186 122.6815 10.18362
MIR-125b-2 328.655 130.2673 217.4513 313.9123 127.6051 62.26342
MIR-126 31.04462 231.5804 964.5492 103.8981 197.332 38.07505
MIR-127 5503.809 11899.7 5537.267 6073.212 7367.812 3427.893
MIR-128a 1913.021 970.5096 506.0228 1211.692 1065.062 467.3048
MIR-128b 322.0318 44.22209 225.0595 741.7277 37.67092 74.71641
MIR-129-2 15514.78 8406.395 10838.85 7713.455 9565.983 3876.494
MIR-130a-P 11760.81 8379.556 10425.72 10587.92 13496.31 4096.524
MIR-130b 277.2207 200.6143 401.3942 233.8788 189.5791 89.73827
MIR-132 7.492078 12.90677 57.81666 34.34195 68.44209 30.39035
Continued
248
Table C.1. continued
MIR-133a-1,2 1014.981 133.8339 535.0988 1261.684 1665.34 340.2207
MIR-133b 1856.095 307.0956 1839.104 1788.284 2072.672 980.5206
MIR-134 5118.074 79.43878 5128.883 4638.395 4291.477 1153.442
MIR-135a-1,2 68.75971 79.38836 125.3644 187.7691 163.1687 32.19183
MIR-135b-P 257.806 66.29544 699.3765 728.8616 263.905 93.29344
MIR-136 51.18809 86.71628 139.9647 121.31 197.1764 39.34398
MIR-137* 8.449507 30.54883 27.82634 27.35567 41.46985 0
MIR-138-1,2 58.17899 159.7229 133.4808 85.87914 154.979 12.28462
MIR-139 2263.82 1804.829 2795.394 2623.898 1877.469 1068.205
MIR-140 0.930443 0 1.798528 2.253947 1.814974 0
MIR-141 113.3429 195.1747 199.0103 275.1045 229.4861 58.2686
MIR-142-P 4970.718 4914.531 3629.009 6598.056 8289.224 1643.344
MIR-143 190.3961 175.2943 698.4452 602.3399 622.3735 324.4794
MIR-144 8.099548 11.95214 117.7107 8.643246 7.571883 0
MIR-145-P 164.0109 674.885 827.5368 697.4703 1059.785 406.4187
MIR-146 35.93817 27.10695 29.37064 78.74723 89.44939 7.230668
MIR-147 225.4706 253.7893 323.8461 365.772 430.9656 77.8308
MIR-148a-P 123.2298 104.3512 169.3357 247.605 123.3789 45.51435
MIR-148b-P 986.9186 877.0721 368.0117 380.3213 959.9428 292.5218
MIR-149 2042.878 987.4495 2554.727 2603.416 3547.527 1330.131
MIR-150 672.3065 598.6471 1117.75 1290.867 993.7567 419.0044
MIR-151 352.4521 257.3444 625.6778 525.5154 339.3763 336.8306
MIR-152 152.3424 29.09152 47.69829 132.6514 150.5859 63.4437
MIR-153-1,2 111.7338 78.28592 89.37798 257.7483 35.23463 26.89646
MIR-154 32.07609 57.96254 65.24155 81.0974 89.90831 22.71621
MIR-155 66.21983 69.26097 44.91643 102.4712 190.5929 48.37833
MIR-181a,c 374.4215 166.7022 234.3763 522.6439 238.5709 124.0678
MIR-181b-1,2 266.679 296.5336 183.5354 632.9201 255.8292 69.49202
MIR-182 874.1759 510.1722 6538.934 2105.695 229.1402 1060.095
MIR-183 111.1306 7.169283 281.7026 64.65016 110.2007 17.91935
MIR-184 2764.261 2816.466 2538.674 1695.443 2345.092 1888.283
MIR-185 2820.113 3900.864 5143.746 5087.112 7930.047 2022.923
MIR-186-P 8687.487 3596.165 2774.455 5004.273 5556.093 8091.426
MIR-187 172.457 202.4664 213.9742 375.2911 270.0591 80.67414
MIR-188b-P 307.3053 96.97499 389.0439 516.829 599.2478 95.60117
MIR-190 8.40962 9.038517 7.304781 22.14138 30.37547 1.281402
MIR-191 59.72927 127.677 146.4452 83.26219 127.9571 12.38503
MIR-192 1274.346 0 658.2719 1142.952 1303.27 410.5423
MIR-193 297.0796 32.63499 444.3399 407.9212 518.7426 158.4782
MIR-194-1,2 110.122 9.022362 205.3603 234.9899 270.2057 73.97635
MIR-195-P 12890.37 8651.802 8004.317 17193.54 22818.52 7326.805
Continued
249
Table C.1. continued
MIR-196a-1 373.5948 524.8424 624.9976 29.92536 927.8567 190.3845
MIR-196a-2 308.9209 377.6943 425.1981 579.8911 696.5027 209.0003
MIR-196b 1188.553 526.462 1514.445 1269.183 1990.884 4574.995
MIR-197 9561.154 6715.475 10936.72 8552.249 10430.31 839.5568
MIR-198 10.25485 27.23975 7.496891 20.50808 31.81037 3.381763
MIR-199a-1-P 3532.271 3122.76 2012.811 5307.241 5745.302 2183.591
MIR-199a-2-P 331.3024 14.70907 329.9027 568.1804 559.7591 193.8401
MIR-199b-P 4247.27 4209.361 4464.392 6410.907 5633.432 4673.392
MIR-200a-P 114526.1 97963.03 103183.2 66872.43 44753.15 45100.52
MIR-200b 93.98931 164.9444 187.893 172.5996 136.6537 2.442379
MIR-200c 227.6598 148.6829 293.986 300.8705 472.2938 59.00694
MIR-203 70.12542 30.84829 234.1509 160.0123 121.1458 18.54053
MIR-204 2741.664 1138.05 4929.028 4018.32 4515.916 875.1448
MIR-205 120.4677 61.92127 72.27868 91.26884 159.6102 29.07442
MIR-206 360.8429 143.2401 450.0692 96.63232 492.1118 141.9149
MIR-208 156.3394 17.50686 112.0073 180.5634 299.7183 61.85746
MIR-210 15574.55 16447.28 30876.33 24397.38 30757.05 8070.606
MIR-211 5522.785 4861.41 7371.303 10298.18 8073.044 7495.651
MIR-212 972.1366 425.6314 1344.729 935.1716 1130.86 602.2588
MIR-213 335.0652 437.0121 268.0511 1623.766 559.5108 92.23252
MIR-214 3839.569 3477.23 4920.847 5002.451 6113.85 2091.734
MIR-215 58.6363 95.30843 213.3499 179.9374 13.28411 97.30162
MIR-216 440.2898 549.3449 395.9147 565.4973 764.8198 234.5174
MIR-217 13.00112 4.448138 19.72684 14.59379 27.97344 4.222659
MIR-218-1,2 56.61873 28.3997 138.224 80.14827 11.04128 176.8762
MIR-219-1,2 201.0228 52.64486 181.6867 128.6737 121.7531 101.3339
MIR-220 444.9044 126.8869 610.8679 436.9656 433.458 176.3589
MIR-221 62.66685 187.8869 88.28524 101.7863 85.09758 382.4978
MIR-222-P 185.3001 239.6429 183.5392 376.9927 348.161 464.8981
MIR-223 78.67755 330.7864 208.076 196.1029 99.57811 43.05095
MIR-224 348.7584 343.0587 582.8114 531.6224 550.543 384.9217
MIR-296 564.9293 521.3971 741.983 896.1399 964.069 521.3143
MIR-299-P 1545.776 1356.846 1304.308 1663.249 813.0071 1309.007
MIR-301 770.6185 197.7101 557.6147 889.8942 522.8079 333.8545
MIR-302 77.66245 78.27097 154.8214 134.8323 159.654 21.95135
MIR-302b 223.9796 186.7529 234.4275 393.7961 516.4862 190.8893
MIR-302c 289.5766 317.166 445.3348 163.2359 618.8807 134.7958
MIR-302d 232.6644 362.3634 308.6238 450.1622 585.1192 96.48595
MIR-320 847.312 485.0178 693.0725 728.369 1305.679 288.541
MIR-321 13324.32 4899.416 22267.29 14451.83 8919.571 5974.062
MIR-323 298.1843 204.215 88.25127 597.1822 472.5881 102.2437
Continued
250
Table C.1 continued
MIR-324-P 2759.529 720.3999 1307.927 1801.631 2393.748 532.4331
MIR-325 152.4464 132.9968 146.3717 303.4011 240.979 18.63928
MIR-326-P 58.71795 84.53467 217.3564 192.9703 167.4323 65.94607
MIR-328 3873.451 1046.446 5290.15 6757.513 7758.295 2358.274
MIR-330 819.0916 27.09692 876.5171 1144.065 487.4847 307.855
MIR-331-P 1101.107 773.4277 852.2676 922.7296 1295.376 424.9777
MIR-335 3.411301 0.704119 29.35963 36.84565 33.96236 7.469318
MIR-337 1066.353 415.411 1622.734 1283.364 1991.248 386.1977
MIR-338 1234.583 1096.385 1766.293 2114.484 2179.54 852.4476
MIR-339 1759.958 1660.233 1366.792 1414.341 1567.968 754.5532
MIR-340 37.81213 650.7559 409.9121 1623.347 125.2216 322.266
MIR-342 590.5115 230.8284 705.2253 550.0349 1126.927 369.7657
MIR-345 3885.185 2094.946 5356.345 5017.119 7266.97 1890.927
MIR-346 2337.821 3251.396 2949.508 3453.948 3900.961 1428.855
MIR-361 242.3455 137.3803 270.5726 314.1634 376.7746 109.1156
MIR-367 39.46731 12.85456 22.22441 21.31749 28.22367 2.299073
MIR-368 5.713689 1.386273 29.81746 10.63905 9.435455 6.089259
MIR-369 0 0 0 2.179649 0 0
MIR-370 297.309 8.653101 461.9151 408.771 493.3364 207.6563
MIR-371 1938.035 2147.472 2155.386 4056.725 3646.81 3873.5
MIR-372 6.847668 12.55147 25.82391 31.65229 23.07713 8.65932
MIR-373 398.5249 430.5096 373.6562 727.1402 798.8929 170.4009
MIR-374-P 13.53244 1.007478 97.60728 61.02578 151.5996 4.252739
MIR-375 55.88773 74.08621 1.445392 148.4851 161.4004 34.45429
MIR-376 8.111453 8.306807 103.5633 56.44974 85.15578 10.84863
MIR-377 184.5237 73.34587 400.0104 699.9629 719.1905 133.0643
MIR-378 181.8826 205.7557 383.0841 610.5676 627.5236 344.9614
MIR-379 6.712977 16.94751 8.575964 7.988344 13.52819 18.71345
MIR-380 14.51789 4.140085 39.19494 41.00681 74.84995 7.934333
MIR-381 27.94209 0.606482 61.25342 78.07433 80.59869 47.75855
MIR-382 65.98568 77.54664 89.94332 176.4227 63.18845 13.7173
MIR-383 206.4342 149.7354 350.5311 298.1358 554.0786 101.8361
MIR-384 0 0 0 0 2.908049 0
MIR-422b 861.6428 1483.341 1463.262 1101.987 1367.448 520.5563
MIR-423 231.5228 206.3646 412.1263 539.5416 430.4981 493.5497
MIR-424 78.38365 37.26375 82.77558 179.1021 102.518 99.51117
MIR-425 764.9245 582.1007 136.7403 247.8503 232.1649 40.42695
251
Lung Cancer
miRNA H23 H522 HOP62 A549 H719
hsa-let-7a 899.88 1530.13 2043.04 868.86 1373.08
hsa-let-7b 682.35 5733.94 7066.97 1891.98 1257.43
hsa-let-7c 211.92 9700.35 399.42 277.45 468.20
hsa-let-7d 158.69 268.77 87.27 55.03 30.14
hsa-let-7e 157.39 120.78 198.15 118.63 100.83
hsa-let-7g 1252.68 2000.31 1310.87 485.38 343.36
hsa-let-7i 392.94 503.21 629.73 485.35 71.92
hsa-miR-001 14.43 0.00 8.22 0.00 0.00
hsa-miR-007 914.32 745.16 81.01 269.64 415.48
hsa-miR-009 2276.04 3002.72 404.75 1347.05 1958.56
hsa-miR-009* 270.66 361.76 42.31 262.34 193.83
hsa-miR-010a 76.77 24.74 1873.87 668.02 1418.74
hsa-miR-010b 9.45 42.60 128.45 4.40 127.67
hsa-miR-015a 288.22 144.60 424.57 219.49 332.83
hsa-miR-015b 8832.73 8907.93 8829.58 3857.78 4753.76
hsa-miR-016 35541.03 29566.27 41832.45 18083.14 22618.47
hsa-miR-017-3p 47.54 6.75 32.37 8.17 13.75
hsa-miR-017-5p 1386.56 6338.38 1641.61 950.11 2262.56
hsa-miR-018 302.15 288.13 335.55 151.83 437.38
hsa-miR-019a 3122.63 5467.28 4701.67 2601.78 4276.36
hsa-miR-019b 14569.79 44195.40 22689.42 11846.99 18958.50
hsa-miR-020 17268.10 24522.55 22197.05 12957.76 24890.93
hsa-miR-021 7589.28 11119.87 12348.62 10244.70 3374.18
hsa-miR-022 176.62 228.39 479.17 245.50 153.97
hsa-miR-023a 187.84 243.34 154.58 69.30 119.97
hsa-miR-023b 77.30 68.23 336.52 570.72 20.97
hsa-miR-023bN 968.30 1778.11 5367.13 10278.06 1470.27
hsa-miR-024 47088.89 62228.03 37424.15 38647.31 42904.20
hsa-miR-025 2257.41 2990.67 1597.18 869.60 1967.47
hsa-miR-026aN 5460.20 9092.17 6596.03 2586.12 5159.77
hsa-miR-026b 4254.76 7004.17 3064.66 1900.34 4309.97
hsa-miR-026bN 2965.01 3492.98 1513.02 983.71 2129.98
hsa-miR-027a 4080.64 5572.63 4895.00 2999.93 6723.94
hsa-miR-027b 235.69 259.19 816.93 3375.89 243.33
hsa-miR-027bN 2014.61 1243.44 5104.12 18497.50 1177.00
hsa-miR-028 982.45 820.74 697.78 497.96 546.20
Continued
Table C.2. Mature microRNA expression in 37 different human cancer cell lines. All data was generated using real-time PCR and TaqMan microRNA assays (Applied
Biosystems). Data is presented as copies of microRNA per cell.
252
Table C.2. continued
hsa-miR-029a 5210.51 865.32 5425.87 1654.53 1389.46
hsa-miR-029b 124.48 44.42 125.25 55.31 28.95
hsa-miR-029c 338.56 63.64 366.92 167.27 119.81
hsa-miR-030a-3p 1948.95 17239.55 5124.64 2063.07 3412.84
hsa-miR-030c 10482.69 44316.76 11935.75 6665.34 11291.55
hsa-miR-030d 2171.37 905.56 833.59 672.53 315.54
hsa-miR-030e-3p 529.48 1536.30 576.82 272.12 1276.27
hsa-miR-031 9374.28 77314.37 23959.23 26433.63 38187.87
hsa-miR-032 284.37 122.99 96.07 116.80 81.95
hsa-miR-033 0.00 0.00 0.00 0.00 0.00
hsa-miR-034a 55.35 1.73 14.43 53.27 0.00
hsa-miR-034aN 656.10 39.47 306.20 588.23 19.31
hsa-miR-034b 21.88 0.00 15.61 13.61 12.18
hsa-miR-034b 13.42 0.00 13.53 0.00 10.44
hsa-miR-092N 12724.76 31479.87 15012.67 7909.82 14757.47
hsa-miR-093 25915.10 35420.31 12044.02 8901.47 21471.88
hsa-miR-095 90.45 298.60 0.00 26.64 73.00
hsa-miR-096 16047.13 11664.91 9902.69 14110.78 15808.94
hsa-miR-098 353.87 870.82 397.23 124.56 35.27
hsa-miR-099a 336.29 11836.72 22408.06 3649.68 5546.68
hsa-miR-099b 2355.45 2203.28 2057.37 1517.41 1783.86
hsa-miR-100 211.08 6707.09 22531.58 3685.07 5723.36
hsa-miR-101 3.97 65.46 55.38 31.29 20.71
hsa-miR-103 1273.12 2325.36 1586.77 671.09 1084.05
hsa-miR-105 3.82 0.00 0.00 0.00 0.00
hsa-miR-106a 2608.37 7696.12 1878.89 2230.82 2979.23
hsa-miR-106b 4494.90 6028.76 2247.55 1680.11 3246.68
hsa-miR-107 26.34 89.90 7.93 13.19 36.93
hsa-miR-108 0.00 0.00 0.00 0.00 0.00
hsa-miR-122a 0.00 0.00 0.00 0.00 0.00
hsa-miR-124a 0.00 0.00 0.00 0.00 0.00
hsa-miR-125a 10142.80 13307.54 15530.30 8528.33 8695.46
hsa-miR-125b 337.48 19256.93 11525.70 1808.52 10964.15
hsa-miR-126 1600.08 2028.18 864.25 951.45 1059.80
hsa-miR-126* 323.29 340.17 129.18 158.65 182.10
hsa-miR-127 1071.81 36.15 530.14 55.70 27.64
hsa-miR-128a 0.00 0.00 0.00 0.00 0.00
hsa-miR-130a 712.60 3509.15 1127.11 405.60 1384.88
hsa-miR-130aN 2081.01 8593.69 3133.12 1002.93 3124.95
hsa-miR-130b 370.06 1647.11 948.81 167.24 979.26
hsa-miR-132 3865.34 1802.05 1044.98 828.13 2500.11
Continued
253
Table C.2. continued
hsa-miR-133a 0.00 0.00 0.00 0.00 6.96
hsa-miR-134 327.64 3.35 125.74 18.27 4.80
hsa-miR-135a 12.88 3.69 0.00 0.00 2.92
hsa-miR-135b 19.75 21.80 97.17 12.45 6.23
hsa-miR-138 0.00 0.00 0.00 14.09 0.00
hsa-miR-139 27.01 0.00 0.00 0.00 0.00
hsa-miR-140 816.52 899.06 1851.63 984.77 51.11
hsa-miR-141 15.74 0.00 4.82 9.18 0.00
hsa-miR-142-3p 0.00 0.00 9.63 20.60 8.15
hsa-miR-142-5p 3.22 12.62 20.14 6.30 6.05
hsa-miR-143 0.00 0.00 0.00 2.53 0.00
hsa-miR-145 4.03 9.44 10.62 1.88 4.75
hsa-miR-146 807.79 432.21 23325.90 408.86 317.25
hsa-miR-146b 326.66 674.33 670.90 115.09 180.44
hsa-miR-147 0.00 0.00 0.00 0.00 0.00
hsa-miR-148a 221.24 58.35 125.54 41.11 128.70
hsa-miR-148b 345.77 318.59 120.85 145.21 179.16
hsa-miR-149 461.03 6836.16 640.35 676.19 1738.99
hsa-miR-150 17.97 133.18 0.00 17.33 16.80
hsa-miR-152 486.99 486.31 1016.58 526.32 57.73
hsa-miR-152N 512.87 432.28 776.35 396.84 87.71
hsa-miR-153 0.00 0.00 0.00 0.00 0.00
hsa-miR-154 0.00 0.00 0.00 0.00 0.00
hsa-miR-154* 0.00 0.00 0.00 0.00 0.00
hsa-miR-155 233.98 7.13 1930.77 28.28 3.70
hsa-miR-181a 535.97 1370.30 958.41 70.21 139.62
hsa-miR-181b 2235.91 3401.72 2640.64 479.14 939.75
hsa-miR-181c 55.71 95.80 48.88 12.60 16.99
hsa-miR-182* 36.81 82.00 3.22 21.30 29.23
hsa-miR-183 920.69 3190.40 171.59 261.55 917.14
hsa-miR-184 0.00 18.51 0.00 0.00 12.23
hsa-miR-186 3956.10 6427.62 1191.90 1347.47 3909.96
hsa-miR-187 3.38 0.00 0.00 0.00 0.00
hsa-miR-188 39.51 55.79 41.90 53.28 49.86
hsa-miR-190 0.00 0.00 0.00 0.00 0.00
hsa-miR-191 9396.24 21765.42 7137.11 2799.44 5871.74
hsa-miR-192 130.97 571.15 370.89 12855.43 173.43
hsa-miR-193 327.89 93.37 470.84 162.67 222.38
hsa-miR-194 42.36 130.47 105.19 2401.70 40.74
hsa-miR-195 944.74 372.59 407.90 267.23 212.38
hsa-miR-196a 1583.10 1966.47 529.34 511.12 2004.00
Continued
254
Table C.2. continued
hsa-miR-196b 0.00 0.00 0.00 0.00 0.00
hsa-miR-197 1881.88 6168.33 722.61 501.80 1540.31
hsa-miR-199a 0.00 0.00 0.00 0.00 0.00
hsa-miR-199a* 13.01 15.21 5.27 0.00 0.00
hsa-miR-199b 0.00 0.00 0.00 0.00 0.00
hsa-miR-199-s 0.00 0.00 0.00 0.00 0.00
hsa-miR-200a 0.00 0.00 244.34 279.06 0.00
hsa-miR-200b 10.47 0.00 675.85 488.72 10.49
hsa-miR-200bN 10.78 0.00 217.68 158.67 0.00
hsa-miR-200c 326.09 117.89 157.79 231.20 52.97
hsa-miR-200cN 184.14 158.07 176.52 212.95 48.32
hsa-miR-203 633.11 220.46 51.85 25.64 871.36
hsa-miR-204 0.00 2.34 110.79 0.00 8.45
hsa-miR-205 20.59 0.00 0.00 0.00 0.00
hsa-miR-206 0.00 4.41 1.80 1.94 11.55
hsa-miR-208 0.00 0.00 0.00 0.00 0.00
hsa-miR-210 273.82 1338.65 1541.72 408.86 753.17
hsa-miR-210N 469.28 2240.51 2458.81 618.79 1233.28
hsa-miR-211 0.00 0.00 0.00 0.00 0.00
hsa-miR-212 891.54 488.73 366.67 181.64 397.98
hsa-miR-213 18.53 10.16 155.29 10.76 40.12
hsa-miR-214 35.80 99.66 14.40 5.41 29.86
hsa-miR-215 6.52 7.59 14.05 10.54 0.00
hsa-miR-216 0.00 0.00 0.00 0.00 0.00
hsa-miR-217 10.48 0.00 0.00 0.00 9.27
hsa-miR-218 119.45 791.21 1349.43 119.98 293.02
hsa-miR-219 0.00 0.00 7.21 0.00 3.06
hsa-miR-220 0.00 0.00 0.00 0.00 0.00
hsa-miR-221 1932.07 20272.96 5132.26 1986.06 6329.01
hsa-miR-222 10244.92 89173.26 30145.83 14584.82 45016.44
hsa-miR-223 0.00 0.00 9.79 8.91 0.00
hsa-miR-296 1335.45 68.73 237.21 9.27 97.74
hsa-miR-299 0.00 0.00 0.00 0.00 0.00
hsa-miR-301 1465.22 6640.75 1367.05 534.53 2645.06
hsa-miR-302a 32.86 7.04 0.00 0.00 0.00
hsa-miR-302a* 0.00 0.00 0.00 0.00 0.00
hsa-miR-302b 146.12 37.48 3.70 27.90 55.39
hsa-miR-302c 109.03 35.01 0.00 11.03 33.94
hsa-miR-302c* 0.00 0.00 0.00 0.00 0.00
hsa-miR-302d 101.39 11.14 0.00 0.00 6.91
hsa-miR-320 2371.90 5266.80 1440.08 1816.07 4299.30
Continued
255
Table C.2. continued
hsa-miR-321 8269.38 28905.04 7037.49 5219.78 7474.25
hsa-miR-323 146.40 0.00 10.41 0.00 0.00
hsa-miR-324-3p 1101.42 2053.62 541.66 594.70 3650.79
hsa-miR-324-5p 950.17 1577.04 362.96 409.64 3754.22
hsa-miR-325 0.00 0.00 0.00 0.00 0.00
hsa-miR-326 5.21 13.06 0.00 0.00 5.96
hsa-miR-328 1094.99 1435.59 862.58 160.85 356.59
hsa-miR-330 56.31 105.03 74.92 26.16 50.67
hsa-miR-331 3093.78 5102.36 1603.08 2702.55 3416.70
hsa-miR-335 186.40 22.60 329.18 197.52 32.70
hsa-miR-337 0.00 0.00 0.00 0.00 0.00
hsa-miR-338 22.00 13.05 14.43 0.00 33.71
hsa-miR-339 103.89 117.04 67.79 29.25 119.46
hsa-miR-340 155.56 570.79 61.89 138.57 397.98
hsa-miR-342 1785.92 9449.89 1171.69 881.74 2982.02
hsa-miR-345 1082.62 7455.65 1160.13 311.01 1967.32
hsa-miR-346 111.96 32.32 10.51 31.25 43.36
hsa-miR-361 347.33 803.89 343.55 147.04 1029.79
hsa-miR-367 12.17 8.22 0.00 0.00 0.00
hsa-miR-368 0.00 0.00 0.00 0.00 0.00
hsa-miR-369 0.00 0.00 0.00 0.00 0.00
hsa-miR-370 587.51 142.34 351.36 126.68 137.22
hsa-miR-371 0.00 0.00 0.00 0.00 0.00
hsa-miR-372 163.46 200.08 124.68 149.12 234.75
hsa-miR-373 0.00 0.00 0.00 4.29 0.00
hsa-miR-374 3308.46 5076.11 2478.66 3497.09 3059.69
hsa-miR-375 331.62 597.33 17.06 146.22 318.68
hsa-miR-376a 548.37 26.52 159.70 15.51 4.18
hsa-miR-377 0.00 0.00 0.00 0.00 0.00
hsa-miR-378 96.36 203.38 13.50 32.00 29.07
hsa-miR-379 423.49 15.97 83.52 21.45 3.70
hsa-miR-380-3P 0.00 0.00 0.00 0.00 0.00
hsa-miR-380-5p 7.66 0.00 0.00 0.00 0.00
hsa-miR-381 9.58 0.00 0.00 0.00 0.00
hsa-miR-382 678.89 25.90 200.50 31.66 23.58
hsa-miR-383 0.00 0.00 0.00 0.00 1.64
hsa-miR-384 0.00 0.00 0.00 0.00 0.00
hsa-miR-422b 53.43 162.05 7.39 44.75 35.60
hsa-miR-423 643.72 518.82 557.33 497.64 538.65
hsa-miR-425 359.13 1755.68 271.38 81.56 361.66
let-7f(mmu) 0.00 0.00 0.00 0.00 0.00
Continued
256
Table C.2. continued
miR-129-5p(mmu) 0.00 0.00 0.00 0.00 0.00
miR-137(mmu) 25.77 1012.22 158.65 77.00 251.79
miR-224(mmu) 17.03 0.00 177.18 196.26 0.00
miR-424(mmu) 0.00 0.00 0.00 0.00 0.00
Pancreas Cancer
miRNA PANC-1 HS766T MIAPACA-2 MPANC-96 PL45
hsa-let-7a 1182.55 1683.50 835.05 1199.63 947.23
hsa-let-7b 2018.23 2560.34 2687.39 3449.97 3574.74
hsa-let-7c 61.33 147.09 94.19 157.31 133.46
hsa-let-7d 7.42 107.35 127.44 116.36 60.07
hsa-let-7e 33.79 61.76 38.89 44.69 65.62
hsa-let-7g 575.71 1589.73 606.07 1480.28 433.40
hsa-let-7i 161.88 675.05 539.08 766.99 87.46
hsa-miR-001 292.52 0.00 0.00 0.00 0.00
hsa-miR-007 108.56 1385.86 316.94 302.28 354.06
hsa-miR-009 1019.56 0.00 47.10 30.47 283.82
hsa-miR-009* 89.09 3.87 11.47 15.05 119.81
hsa-miR-010a 4240.69 475.29 720.21 4866.12 973.01
hsa-miR-010b 284.96 48.55 3.20 212.17 10.76
hsa-miR-015a 186.19 454.62 266.51 239.80 162.86
hsa-miR-015b 7882.98 6493.87 7885.11 4970.63 7631.24
hsa-miR-016 4756.25 5654.60 4773.09 5100.24 5445.30
hsa-miR-017-3p 11.83 35.42 19.03 50.96 13.73
hsa-miR-017-5p 1049.42 1889.16 1021.35 951.88 550.43
hsa-miR-018 89.39 498.54 170.61 107.24 35.40
hsa-miR-019a 1337.52 8647.92 3837.72 2913.71 1273.45
hsa-miR-019b 7793.51 31589.36 12470.07 15457.90 7010.03
hsa-miR-020 10079.63 36529.78 14265.01 17326.28 7772.59
hsa-miR-021 3874.18 12073.29 3337.03 6557.24 3111.97
hsa-miR-022 99.85 94.40 111.69 61.83 32.83
hsa-miR-023a 65.18 78.86 82.39 75.64 114.99
hsa-miR-023b 118.28 80.38 42.16 425.54 32.55
hsa-miR-023bN 2953.85 735.94 380.00 6726.38 808.58
hsa-miR-024 21153.36 17003.19 21389.59 31790.52 15809.04
hsa-miR-025 974.48 2118.61 1012.44 1279.18 902.75
hsa-miR-026aN 2863.57 4158.32 1293.18 7484.30 2664.44
hsa-miR-026b 1759.62 2873.32 1183.59 4609.94 1817.11
hsa-miR-026bN 784.68 1403.41 240.49 2147.10 0.00
hsa-miR-027a 2026.78 2420.29 3194.50 2089.69 2018.47
hsa-miR-027b 286.88 133.38 51.72 1003.37 99.24
Continued
257
Table C.2. continued
hsa-miR-027bN 2623.94 2748.54 460.93 8239.67 817.18
hsa-miR-028 665.30 765.12 409.52 684.17 413.53
hsa-miR-029a 2512.60 11118.61 3791.49 4390.23 3388.84
hsa-miR-029b 42.19 505.17 161.04 68.10 51.57
hsa-miR-029c 158.34 882.52 196.22 313.04 216.05
hsa-miR-030a-3p 2984.91 26.16 2582.91 1402.86 2014.20
hsa-miR-030c 8502.08 2950.37 4129.02 6620.50 5378.40
hsa-miR-030d 699.28 1127.14 181.31 1287.66 605.23
hsa-miR-030e-3p 474.62 213.62 188.63 283.53 141.34
hsa-miR-031 20261.73 27292.91 10.07 19862.04 36109.44
hsa-miR-032 68.16 265.80 171.55 57.39 45.96
hsa-miR-033 1.91 0.00 14.67 0.00 4.98
hsa-miR-034a 17.16 3.21 0.00 1.43 0.00
hsa-miR-034aN 67.52 148.35 0.00 0.00 19.57
hsa-miR-034b 14.26 0.00 13.02 0.00 0.00
hsa-miR-034b 2.31 0.00 10.89 0.00 0.00
hsa-miR-092N 9037.87 16595.75 8363.07 11399.15 6042.37
hsa-miR-093 9014.69 22378.67 12604.49 10082.93 7896.46
hsa-miR-095 12.14 46.01 32.00 38.50 43.70
hsa-miR-096 9105.72 17587.77 5416.77 6678.06 10488.34
hsa-miR-098 117.64 353.18 146.97 117.58 151.38
hsa-miR-099a 7302.87 6044.46 5.00 1725.94 1334.53
hsa-miR-099b 552.64 804.48 490.45 407.92 520.17
hsa-miR-100 9246.94 5731.50 0.00 1797.79 1447.18
hsa-miR-101 3.83 57.34 14.75 11.03 12.21
hsa-miR-103 674.71 1540.66 592.86 702.67 389.51
hsa-miR-105 0.00 0.00 0.00 0.00 0.00
hsa-miR-106a 1537.85 3331.72 698.90 1249.47 968.86
hsa-miR-106b 1218.82 4992.36 2116.40 1373.32 780.97
hsa-miR-107 0.00 2.68 2.95 2.56 0.00
hsa-miR-108 0.00 0.00 0.00 0.00 0.00
hsa-miR-122a 0.00 0.00 0.00 0.00 0.00
hsa-miR-124a 0.00 0.00 0.00 0.00 0.00
hsa-miR-125a 3963.27 5238.75 2715.63 3949.27 4428.31
hsa-miR-125b 3415.95 1718.28 0.00 1174.38 1014.06
hsa-miR-126 1132.37 5267.51 1653.84 1576.51 350.67
hsa-miR-126* 155.59 863.15 219.04 217.85 61.64
hsa-miR-127 2672.65 0.00 0.00 0.00 0.00
hsa-miR-128a 0.00 0.00 0.00 0.00 0.00
hsa-miR-130a 336.84 13.02 412.84 4.32 133.14
hsa-miR-130aN 788.39 0.00 1115.50 9.43 274.30
Continued
258
Table C.2. continued
hsa-miR-130b 32.89 107.90 9.01 32.33 3.69
hsa-miR-132 734.05 633.57 482.70 310.17 148.36
hsa-miR-133a 70.10 3.36 0.00 0.00 6.33
hsa-miR-134 450.08 4.06 0.00 0.00 0.00
hsa-miR-135a 13.35 15.45 25.52 29.95 20.90
hsa-miR-135b 114.52 399.82 551.19 683.13 740.04
hsa-miR-138 0.00 6.34 0.00 0.00 0.00
hsa-miR-139 0.00 0.00 0.00 0.00 2.81
hsa-miR-140 574.24 2213.56 1515.36 843.81 186.15
hsa-miR-141 7.19 12.43 3.56 166.63 436.46
hsa-miR-142-3p 0.00 7.74 0.00 0.00 0.00
hsa-miR-142-5p 6.10 5.83 3.52 12.03 3.01
hsa-miR-143 0.00 0.00 0.00 0.00 0.00
hsa-miR-145 1.48 7.73 0.00 9.18 11.31
hsa-miR-146 2767.86 440.78 329.94 1736.16 358.23
hsa-miR-146b 307.98 89.65 31.06 90.41 143.28
hsa-miR-147 0.00 0.00 0.00 0.00 0.00
hsa-miR-148a 10.84 321.30 26.82 0.00 23.25
hsa-miR-148b 149.41 203.48 82.41 124.85 42.80
hsa-miR-149 640.38 127.63 582.41 532.51 325.17
hsa-miR-150 7.26 15.00 14.54 573.32 15.54
hsa-miR-152 420.73 859.88 312.81 278.44 165.61
hsa-miR-152N 386.15 820.70 260.12 151.55 91.24
hsa-miR-153 0.00 0.00 0.00 0.00 0.00
hsa-miR-154 0.00 0.00 0.00 0.00 0.00
hsa-miR-154* 0.00 0.00 0.00 0.00 0.00
hsa-miR-155 696.79 2925.00 15.02 441.07 305.47
hsa-miR-181a 136.36 23.81 0.00 692.14 81.81
hsa-miR-181b 976.10 364.42 144.23 2863.26 568.84
hsa-miR-181c 17.24 57.14 3.94 45.83 12.96
hsa-miR-182* 9.89 33.83 7.04 4.05 11.08
hsa-miR-183 335.73 774.19 548.89 333.20 205.91
hsa-miR-184 21.24 0.00 0.00 0.00 0.00
hsa-miR-186 1818.11 2979.35 2915.06 1770.43 845.94
hsa-miR-187 185.47 0.00 15.43 0.00 0.00
hsa-miR-188 24.66 32.51 21.27 42.82 22.66
hsa-miR-190 0.00 0.00 0.00 0.00 0.00
hsa-miR-191 9238.01 6156.52 3770.53 4960.73 1719.16
hsa-miR-192 146.83 390.13 260.08 20261.48 99.27
hsa-miR-193 274.19 64.76 178.42 73.95 75.03
hsa-miR-194 76.88 107.24 58.69 5518.49 40.59
Continued
259
Table C.2. continued
hsa-miR-195 356.35 240.35 235.86 260.83 226.77
hsa-miR-196a 2765.94 613.40 33.76 2339.81 17.20
hsa-miR-196b 0.00 20.83 0.00 192.75 0.00
hsa-miR-197 1125.13 793.34 1129.59 686.90 503.16
hsa-miR-199a 0.00 0.00 0.00 0.00 0.00
hsa-miR-199a* 0.00 0.00 0.00 0.00 4.77
hsa-miR-199b 0.00 0.00 0.00 0.00 0.00
hsa-miR-199-s 0.00 0.00 0.00 0.00 0.00
hsa-miR-200a 9.16 2399.83 0.00 1370.77 644.17
hsa-miR-200b 20.89 2723.08 11.64 3772.93 1961.98
hsa-miR-200bN 10.02 1633.16 4.31 1707.55 891.62
hsa-miR-200c 50.45 215.69 45.16 6084.19 16196.05
hsa-miR-200cN 36.64 18.28 14.49 5835.24 16427.93
hsa-miR-203 13.20 2956.58 0.00 22.04 578.34
hsa-miR-204 0.00 0.00 0.00 5.58 0.00
hsa-miR-205 0.00 0.00 0.00 0.00 319.45
hsa-miR-206 5.57 4.04 11.87 0.00 0.00
hsa-miR-208 0.00 0.00 0.00 0.00 0.00
hsa-miR-210 261.56 139.95 149.59 538.87 115.30
hsa-miR-210N 558.89 361.26 237.42 1120.24 276.29
hsa-miR-211 0.00 0.00 2.38 0.00 0.00
hsa-miR-212 201.69 173.23 115.82 108.38 109.83
hsa-miR-213 43.03 14.81 0.00 152.26 14.35
hsa-miR-214 12.43 2.87 0.00 9.31 15.35
hsa-miR-215 19.19 8.99 0.00 76.54 0.00
hsa-miR-216 0.00 0.00 0.00 0.00 0.00
hsa-miR-217 0.00 0.00 1.39 0.00 6.57
hsa-miR-218 55.10 1.78 10.38 0.00 12.71
hsa-miR-219 0.00 0.00 0.00 0.00 0.00
hsa-miR-220 0.00 0.00 0.00 0.00 0.00
hsa-miR-221 2305.77 6273.71 6879.89 2067.47 3075.95
hsa-miR-222 13809.56 41793.55 36747.91 21292.04 19678.62
hsa-miR-223 6.92 1.71 8.75 16.70 0.00
hsa-miR-296 0.00 9.19 12.90 4.63 4.21
hsa-miR-299 0.00 0.00 0.00 0.00 0.00
hsa-miR-301 395.72 1346.47 726.16 336.56 189.55
hsa-miR-302a 0.00 0.00 0.00 0.00 0.00
hsa-miR-302a* 0.00 0.00 0.00 0.00 0.00
hsa-miR-302b 15.99 11.53 8.41 4.01 20.14
hsa-miR-302c 0.00 0.00 0.00 20.14 0.00
hsa-miR-302c* 0.00 0.00 0.00 0.00 0.00
Continued
260
Table C.2. continued
hsa-miR-302d 0.00 0.00 0.00 0.00 0.00
hsa-miR-320 874.67 1589.50 3647.10 3405.09 727.09
hsa-miR-321 10347.40 6568.02 5043.85 8845.34 4634.01
hsa-miR-323 42.17 0.00 0.00 0.00 0.00
hsa-miR-324-3p 791.67 1690.38 803.86 584.20 205.87
hsa-miR-324-5p 291.56 1418.81 423.34 243.12 47.40
hsa-miR-325 2.34 3.52 1.68 2.27 3.16
hsa-miR-326 0.00 11.19 2.67 5.11 0.00
hsa-miR-328 110.91 306.86 216.12 262.31 78.76
hsa-miR-330 13.27 36.31 24.07 15.19 5.90
hsa-miR-331 2383.78 1651.38 2121.01 2371.51 1707.61
hsa-miR-335 210.79 156.04 0.00 45.66 102.41
hsa-miR-337 0.00 0.00 0.00 0.00 0.00
hsa-miR-338 0.00 0.00 15.23 0.00 13.62
hsa-miR-339 38.70 44.73 66.02 52.40 45.39
hsa-miR-340 127.84 159.50 119.79 89.82 58.09
hsa-miR-342 1470.67 10.95 0.00 17.08 0.00
hsa-miR-345 298.80 1182.03 700.93 217.21 35.67
hsa-miR-346 7.77 14.06 0.00 10.51 7.68
hsa-miR-361 228.49 285.02 165.98 377.80 169.85
hsa-miR-367 0.00 0.00 0.00 0.00 0.00
hsa-miR-368 0.00 0.00 0.00 0.00 0.00
hsa-miR-369 0.00 0.00 0.00 0.00 0.00
hsa-miR-370 657.56 27.29 58.76 74.70 38.11
hsa-miR-371 0.00 0.00 0.00 0.00 0.00
hsa-miR-372 0.00 0.00 0.00 0.00 0.00
hsa-miR-373 0.00 0.00 0.00 0.00 0.00
hsa-miR-374 1435.58 2418.16 1653.68 2606.33 521.62
hsa-miR-375 13.71 13.40 38.35 19.96 407.26
hsa-miR-376a 621.81 0.00 0.00 7.40 0.00
hsa-miR-377 5.50 0.00 0.00 0.00 0.00
hsa-miR-378 90.77 65.51 112.36 58.01 23.71
hsa-miR-379 119.11 0.00 0.00 10.57 0.00
hsa-miR-380-3P 0.00 0.00 0.00 0.00 0.00
hsa-miR-380-5p 19.04 0.00 0.00 0.00 0.00
hsa-miR-381 0.00 0.00 0.00 0.00 0.00
hsa-miR-382 900.25 0.00 0.00 5.69 0.00
hsa-miR-383 0.00 0.00 0.00 0.00 3.49
hsa-miR-384 0.00 0.00 0.00 0.00 0.00
hsa-miR-422b 78.19 19.30 34.96 28.43 19.65
hsa-miR-423 594.46 384.58 411.21 332.21 316.29
Continued
261
Table C.2. continued
hsa-miR-425 391.56 110.37 132.61 93.47 32.05
let-7f(mmu) 0.00 0.00 0.00 0.00 0.00
miR-129-5p(mmu) 0.00 0.00 0.00 0.00 0.00
miR-137(mmu) 12.71 0.00 123.57 0.00 0.00
miR-224(mmu) 0.00 0.00 0.00 221.65 0.00
miR-424(mmu) 0.00 0.00 0.00 0.00 0.00
Prostate Cancer
miRNA PC3 PPC1 DU145 TSUPR1 LNCAP
hsa-let-7a 248.64 2305.57 1209.72 1457.35 1891.86
hsa-let-7b 1790.37 4713.23 2376.29 5686.75 21296.95
hsa-let-7c 94.51 445.03 148.22 463.82 16362.12
hsa-let-7d 16.90 98.60 153.70 90.55 146.32
hsa-let-7e 22.58 111.35 32.20 69.12 94.09
hsa-let-7g 155.05 1354.98 1139.74 1011.21 1654.15
hsa-let-7i 90.89 751.05 502.07 445.62 198.57
hsa-miR-001 0.00 0.00 1.68 0.00 0.00
hsa-miR-007 25.23 134.49 507.95 519.90 217.20
hsa-miR-009 324.61 3542.37 760.84 11.93 986.36
hsa-miR-009* 23.69 492.44 115.89 0.00 68.69
hsa-miR-010a 196.65 193.28 1028.68 1526.50 5.93
hsa-miR-010b 0.00 20.39 9.09 161.49 0.00
hsa-miR-015a 36.80 378.10 472.76 414.05 676.86
hsa-miR-015b 998.70 5672.10 6980.34 6572.05 6602.90
hsa-miR-016 1317.36 21365.37 36158.78 27371.26 34778.76
hsa-miR-017-3p 0.00 25.38 27.58 5.78 24.61
hsa-miR-017-5p 105.16 2100.65 1317.66 1852.93 2777.61
hsa-miR-018 26.84 537.88 216.08 235.85 426.26
hsa-miR-019a 169.23 6135.63 2103.06 5012.79 7732.10
hsa-miR-019b 912.67 22847.43 11275.52 23341.12 31302.75
hsa-miR-020 799.78 28984.67 14464.54 25677.52 34089.62
hsa-miR-021 161.84 3557.98 7101.08 8197.32 943.58
hsa-miR-022 8.52 152.68 406.41 175.24 66.77
hsa-miR-023a 26.39 73.95 226.08 101.91 0.00
hsa-miR-023b 18.24 113.72 191.94 21.99 41.05
hsa-miR-023bN 156.25 3098.45 5211.46 1296.45 1011.47
hsa-miR-024 5332.92 27574.56 31197.25 31981.14 3474.57
hsa-miR-025 190.56 1603.58 1303.46 891.75 1825.62
hsa-miR-026aN 671.00 1321.81 3812.02 4712.45 6365.70
hsa-miR-026b 113.08 1578.39 1678.96 4053.37 4864.09
hsa-miR-026bN 64.73 849.93 873.11 1880.25 2076.05
Continued
262
Table C.2. continued
hsa-miR-027a 399.50 3240.49 5464.23 5730.23 237.64
hsa-miR-027b 0.00 500.80 1143.60 213.29 227.69
hsa-miR-027bN 126.21 3143.08 5999.86 1103.12 1262.66
hsa-miR-028 77.08 332.04 893.73 532.26 1459.06
hsa-miR-029a 437.78 1852.21 5349.11 3941.16 424.46
hsa-miR-029b 4.24 80.52 138.22 204.44 37.68
hsa-miR-029c 41.00 180.49 506.00 148.86 485.00
hsa-miR-030a-3p 22.32 40.17 1321.05 3703.89 244.84
hsa-miR-030c 1066.79 2909.14 5676.08 9925.13 5328.64
hsa-miR-030d 229.69 419.32 423.15 195.20 720.92
hsa-miR-030e-3p 26.96 242.41 471.20 772.32 379.05
hsa-miR-031 4701.60 11519.16 30487.37 10080.98 9.58
hsa-miR-032 8.44 179.14 246.44 209.02 316.04
hsa-miR-033 0.00 0.00 0.00 0.00 0.00
hsa-miR-034a 9.53 0.00 71.82 0.00 92.36
hsa-miR-034aN 0.00 4.21 922.96 0.00 1087.61
hsa-miR-034b 0.00 0.00 61.73 0.00 0.00
hsa-miR-034b 0.00 0.00 13.27 0.00 0.00
hsa-miR-092N 1517.93 15305.20 8479.11 12791.92 14952.96
hsa-miR-093 981.55 19938.32 15453.07 7735.01 16114.06
hsa-miR-095 9.49 20.60 32.27 0.00 17.68
hsa-miR-096 6904.79 14288.44 19008.81 9715.03 9993.99
hsa-miR-098 22.71 926.21 204.82 225.49 272.99
hsa-miR-099a 10434.41 26141.84 5529.25 11159.14 15743.31
hsa-miR-099b 1096.16 2115.44 574.02 1002.27 1342.27
hsa-miR-100 9604.95 26646.09 5104.97 12940.54 9477.41
hsa-miR-101 0.00 29.89 27.43 9.52 76.96
hsa-miR-103 188.42 870.50 802.03 880.06 899.45
hsa-miR-105 0.00 18.02 0.00 6.98 27.11
hsa-miR-106a 490.08 2343.29 2995.59 1939.78 2215.90
hsa-miR-106b 164.25 4335.05 2270.95 1317.71 2474.42
hsa-miR-107 0.00 28.06 10.77 9.86 32.99
hsa-miR-108 0.00 0.00 0.00 0.00 0.00
hsa-miR-122a 0.00 0.00 0.00 0.00 0.00
hsa-miR-124a 0.00 0.00 0.00 0.00 0.00
hsa-miR-125a 5550.89 7722.41 3669.96 7011.86 8007.42
hsa-miR-125b 7241.52 9389.55 2272.91 4976.48 18641.08
hsa-miR-126 26.56 480.65 1395.29 220.48 578.73
hsa-miR-126* 26.10 131.47 217.06 53.74 160.96
hsa-miR-127 0.00 0.00 0.00 0.00 0.00
hsa-miR-128a 0.00 0.00 0.00 0.00 0.00
Continued
263
Table C.2. continued
hsa-miR-130a 59.70 8.34 1008.53 1167.46 4.45
hsa-miR-130aN 253.02 6.22 2361.61 2703.81 12.32
hsa-miR-130b 67.57 815.31 381.89 597.61 562.61
hsa-miR-132 83.47 155.89 410.04 762.79 723.04
hsa-miR-133a 0.00 0.00 0.00 0.00 0.00
hsa-miR-134 4.60 10.14 3.41 14.61 2.92
hsa-miR-135a 0.00 0.00 18.29 0.00 6.69
hsa-miR-135b 23.63 13.11 520.24 61.67 0.00
hsa-miR-138 0.00 0.00 29.81 3.30 2.48
hsa-miR-139 13.58 0.00 3.52 0.00 0.00
hsa-miR-140 81.66 911.94 1572.64 1658.06 736.25
hsa-miR-141 0.00 0.00 112.29 0.00 1962.62
hsa-miR-142-3p 0.00 12.70 0.00 10.83 8.02
hsa-miR-142-5p 2.04 7.72 8.71 6.95 6.74
hsa-miR-143 0.00 0.00 0.00 2.11 0.00
hsa-miR-145 0.00 21.47 12.63 4.77 0.00
hsa-miR-146 458.52 372.96 455.69 501.98 404.83
hsa-miR-146b 17.87 74.41 85.26 115.10 739.34
hsa-miR-147 0.00 0.00 0.00 0.00 0.00
hsa-miR-148a 3.15 1.80 221.97 25.91 3034.66
hsa-miR-148b 12.81 236.38 216.50 162.04 342.35
hsa-miR-149 101.46 1453.42 917.41 472.04 584.67
hsa-miR-150 0.00 4.18 0.00 0.00 0.00
hsa-miR-152 11.31 0.00 552.58 60.27 470.38
hsa-miR-152N 78.53 57.93 483.12 81.79 462.79
hsa-miR-153 0.00 0.00 0.00 0.00 0.00
hsa-miR-154 0.00 0.00 0.00 0.00 0.00
hsa-miR-154* 0.00 0.00 0.00 0.00 0.00
hsa-miR-155 8.69 11.37 488.95 6.32 8.54
hsa-miR-181a 27.55 151.57 395.55 179.42 51.82
hsa-miR-181b 230.36 909.47 1532.69 1310.84 283.96
hsa-miR-181c 8.87 13.64 44.56 14.99 2.19
hsa-miR-182* 7.32 4.58 0.00 15.14 15.03
hsa-miR-183 198.15 621.03 403.22 124.21 423.05
hsa-miR-184 0.00 0.00 0.00 0.00 0.00
hsa-miR-186 517.48 3977.23 2416.84 3818.16 5837.81
hsa-miR-187 0.00 0.00 21.14 0.00 0.00
hsa-miR-188 38.13 39.83 33.14 56.44 46.24
hsa-miR-190 0.00 0.00 0.00 0.00 0.00
hsa-miR-191 2949.10 4882.11 5126.32 2695.08 9832.45
hsa-miR-192 10.49 257.61 488.52 215.71 405.10
Continued
264
Table C.2. continued
hsa-miR-193 24.14 355.44 406.64 105.76 196.37
hsa-miR-194 14.08 47.68 120.34 67.56 99.91
hsa-miR-195 24.07 261.41 506.97 243.77 2652.71
hsa-miR-196a 7.25 306.59 929.18 802.49 142.21
hsa-miR-196b 0.00 132.30 11.28 42.61 0.00
hsa-miR-197 827.04 1471.11 796.72 1748.38 2122.89
hsa-miR-199a 0.00 0.00 0.00 0.00 0.00
hsa-miR-199a* 0.00 10.46 18.54 0.00 12.96
hsa-miR-199b 0.00 0.00 0.00 0.00 0.00
hsa-miR-199-s 0.00 0.00 0.00 0.00 0.00
hsa-miR-200a 438.34 0.00 1319.82 414.58 3629.46
hsa-miR-200b 450.01 8.24 4014.36 1179.32 9999.19
hsa-miR-200bN 146.94 0.00 1251.11 327.32 3604.62
hsa-miR-200c 35.16 74.30 3901.39 76.17 41166.56
hsa-miR-200cN 0.00 65.74 4283.23 11.04 35677.28
hsa-miR-203 0.00 0.00 30.68 0.00 1964.66
hsa-miR-204 0.00 0.00 0.00 0.00 0.00
hsa-miR-205 14.31 0.00 0.00 0.00 0.00
hsa-miR-206 0.00 0.00 0.00 0.00 3.63
hsa-miR-208 0.00 0.00 0.00 0.00 0.00
hsa-miR-210 245.92 207.03 921.80 1025.47 2171.44
hsa-miR-210N 454.75 346.15 1745.85 1753.32 3648.52
hsa-miR-211 2.16 0.00 0.00 0.00 0.00
hsa-miR-212 125.78 145.08 255.78 148.78 154.44
hsa-miR-213 0.00 25.90 45.98 23.77 0.00
hsa-miR-214 0.00 18.69 10.07 3.59 4.58
hsa-miR-215 0.00 10.08 2.52 0.00 8.82
hsa-miR-216 0.00 0.00 0.00 0.00 0.00
hsa-miR-217 0.00 0.00 0.00 0.00 0.00
hsa-miR-218 163.87 386.42 24.05 152.93 0.00
hsa-miR-219 0.00 0.00 0.00 0.00 0.00
hsa-miR-220 0.00 0.00 0.00 0.00 0.00
hsa-miR-221 680.25 4111.80 2903.41 4537.31 509.99
hsa-miR-222 8097.75 21467.41 21031.43 27649.99 3340.37
hsa-miR-223 0.00 0.00 0.00 20.30 38.70
hsa-miR-296 2.91 5.01 280.08 18.00 63.51
hsa-miR-299 0.00 0.00 0.00 0.00 0.00
hsa-miR-301 221.87 2116.43 783.40 861.80 1906.77
hsa-miR-302a 0.00 0.00 0.00 0.00 0.00
hsa-miR-302a* 0.00 0.00 0.00 0.00 0.00
hsa-miR-302b 4.78 0.00 15.04 22.93 27.64
Continued
265
Table C.2. continued
hsa-miR-302c 0.00 0.00 0.00 0.00 0.00
hsa-miR-302c* 0.00 0.00 0.00 0.00 0.00
hsa-miR-302d 0.00 0.00 0.00 0.00 0.00
hsa-miR-320 1053.72 2470.85 1721.10 3163.14 2100.71
hsa-miR-321 1495.88 6988.05 7266.64 8810.17 7492.00
hsa-miR-323 0.00 0.00 0.00 0.00 0.00
hsa-miR-324-3p 144.75 696.30 196.62 2110.81 1876.14
hsa-miR-324-5p 153.72 860.59 489.33 1144.39 1267.45
hsa-miR-325 0.00 0.00 0.00 0.00 0.00
hsa-miR-326 0.00 11.37 0.00 1.60 15.09
hsa-miR-328 415.86 129.24 218.75 127.19 538.61
hsa-miR-330 15.40 91.85 13.01 32.01 26.88
hsa-miR-331 1533.35 2960.79 4388.04 2221.64 2578.03
hsa-miR-335 12.29 0.00 266.29 0.00 152.22
hsa-miR-337 0.00 0.00 0.00 0.00 0.00
hsa-miR-338 0.00 0.00 0.00 0.00 0.00
hsa-miR-339 29.65 55.60 90.30 46.57 86.36
hsa-miR-340 19.51 103.84 177.12 91.73 201.86
hsa-miR-342 444.31 3312.30 2566.61 908.06 1626.29
hsa-miR-345 283.42 1004.11 593.95 348.95 940.61
hsa-miR-346 11.62 24.95 16.93 22.04 17.16
hsa-miR-361 29.22 527.75 236.79 264.04 270.05
hsa-miR-367 0.00 0.00 1.55 0.00 0.00
hsa-miR-368 0.00 0.00 0.00 0.00 0.00
hsa-miR-369 0.00 0.00 0.00 0.00 0.00
hsa-miR-370 109.86 133.32 82.30 69.82 96.83
hsa-miR-371 0.00 0.00 0.00 0.00 0.00
hsa-miR-372 67.28 124.89 91.78 211.41 100.90
hsa-miR-373 0.00 0.00 0.00 4.43 13.40
hsa-miR-374 62.62 2869.91 1750.01 2124.95 2628.65
hsa-miR-375 32.16 38.94 141.10 0.00 417.78
hsa-miR-376a 10.80 11.67 0.00 20.40 0.00
hsa-miR-377 0.00 0.00 0.00 0.00 6.41
hsa-miR-378 0.00 58.24 94.01 41.80 80.50
hsa-miR-379 2.53 21.94 0.00 11.40 10.02
hsa-miR-380-3P 0.00 0.00 0.00 0.00 0.00
hsa-miR-380-5p 0.00 0.00 0.00 14.79 27.08
hsa-miR-381 0.00 0.00 0.00 0.00 0.00
hsa-miR-382 0.00 26.09 0.00 28.16 0.00
hsa-miR-383 0.00 2.29 0.00 0.00 3.45
hsa-miR-384 0.00 0.00 0.00 0.00 0.00
Continued
266
Table C.2. continued
hsa-miR-422b 2.35 90.48 27.68 61.72 37.44
hsa-miR-423 70.66 335.16 647.27 439.82 384.63
hsa-miR-425 88.57 423.28 156.14 184.42 428.14
let-7f(mmu) 0.00 0.00 0.00 0.00 0.00
miR-129-5p(mmu) 0.00 0.00 0.00 0.00 0.00
miR-137(mmu) 0.00 0.00 23.25 649.01 0.00
miR-224(mmu) 0.00 0.00 0.00 0.00 18.08
miR-424(mmu) 0.00 0.00 0.00 0.00 0.00
Head & Neck squamous cell carcinoma
miRNA SCC17A SCCD12 SCC10B SCC17B SCC5
hsa-let-7a 1432.17 1422.63 2492.13 1352.32 1196.91
hsa-let-7b 5603.36 2448.27 3698.29 3766.39 3580.95
hsa-let-7c 293.72 138.95 360.04 200.70 547.20
hsa-let-7d 147.70 119.16 306.49 89.48 87.89
hsa-let-7e 120.12 82.25 345.64 79.65 17.58
hsa-let-7g 1573.21 1468.61 1776.82 929.83 863.15
hsa-let-7i 507.34 325.87 484.10 173.40 339.43
hsa-miR-001 38.40 0.00 0.00 0.00 0.00
hsa-miR-007 1096.74 662.71 284.21 222.43 671.49
hsa-miR-009 967.60 521.56 296.60 1557.24 704.42
hsa-miR-009* 122.00 82.11 36.76 126.10 95.61
hsa-miR-010a 688.40 218.31 780.56 443.15 406.95
hsa-miR-010b 45.67 3.63 16.82 24.24 5.05
hsa-miR-015a 706.67 553.66 735.54 300.12 559.98
hsa-miR-015b 7123.47 8131.90 9440.75 9040.04 5371.61
hsa-miR-016 49138.68 36045.07 46396.61 38182.58 34757.70
hsa-miR-017-3p 37.99 49.39 4.85 7.13 28.89
hsa-miR-017-5p 1025.08 2600.63 1639.36 1354.59 1911.85
hsa-miR-018 177.63 670.32 298.50 136.57 259.13
hsa-miR-019a 3530.79 4763.06 3088.77 2163.89 6580.42
hsa-miR-019b 16523.96 28827.05 12748.53 10503.62 27604.21
hsa-miR-020 22396.19 35690.95 18899.59 13421.80 32642.91
hsa-miR-021 9671.83 5293.54 26677.05 4331.44 7200.48
hsa-miR-022 288.38 340.80 429.22 328.03 240.48
hsa-miR-023a 125.31 228.76 167.66 63.60 152.89
hsa-miR-023b 65.99 42.61 455.77 123.00 314.85
hsa-miR-023bN 1103.36 1465.06 7551.85 2530.85 8578.99
hsa-miR-024 20496.76 35050.50 41223.35 25676.28 32990.65
hsa-miR-025 1060.01 719.49 1708.72 1088.65 1328.36
hsa-miR-026aN 7264.31 4701.94 19974.22 7103.69 5152.03
Continued
267
Table C.2. continued
hsa-miR-026b 4445.90 3161.08 3952.33 3158.05 1648.16
hsa-miR-026bN 2477.70 1654.49 2068.10 1370.63 757.74
hsa-miR-027a 4510.17 5304.75 3926.65 4333.81 6256.32
hsa-miR-027b 256.93 332.92 2097.33 253.65 1835.57
hsa-miR-027bN 1627.44 2167.60 11174.64 2314.35 8291.54
hsa-miR-028 1228.88 676.82 861.53 1401.61 620.13
hsa-miR-029a 7498.57 3000.26 2691.27 2780.28 1103.67
hsa-miR-029b 505.15 94.42 201.56 55.28 73.88
hsa-miR-029c 684.04 252.28 300.06 224.58 162.53
hsa-miR-030a-3p 1524.75 708.62 1061.08 531.08 1373.86
hsa-miR-030c 3662.33 4989.50 14772.29 5544.51 6752.67
hsa-miR-030d 438.93 998.84 3123.83 496.58 528.68
hsa-miR-030e-3p 594.01 486.87 1380.50 512.99 527.08
hsa-miR-031 121187.86 36327.20 38963.94 86345.07 22803.02
hsa-miR-032 403.04 212.18 814.76 19.13 231.46
hsa-miR-033 0.00 0.00 14.58 0.00 0.00
hsa-miR-034a 107.73 14.75 0.00 39.41 18.54
hsa-miR-034aN 1330.28 169.80 149.74 523.74 311.56
hsa-miR-034b 20.13 8.24 25.96 8.18 28.65
hsa-miR-034b 11.27 0.00 15.90 0.00 0.00
hsa-miR-092N 11220.69 20022.99 9013.43 9347.40 16055.06
hsa-miR-093 12665.59 9682.32 22616.87 10025.99 13025.53
hsa-miR-095 43.28 14.41 210.56 191.81 100.70
hsa-miR-096 6325.35 13314.77 30352.78 13673.57 6370.14
hsa-miR-098 241.84 358.11 2038.54 69.87 351.55
hsa-miR-099a 994.25 5509.79 1182.19 522.35 800.10
hsa-miR-099b 1175.21 834.24 2918.64 1380.12 291.74
hsa-miR-100 930.03 6220.09 1076.12 508.77 530.13
hsa-miR-101 45.49 15.40 135.82 47.54 40.57
hsa-miR-103 603.50 754.20 1696.41 928.22 812.93
hsa-miR-105 16.52 15.54 0.00 11.04 22.15
hsa-miR-106a 1351.41 3260.90 3367.12 1902.95 1624.86
hsa-miR-106b 3416.05 1994.43 4845.71 1535.21 2606.35
hsa-miR-107 10.51 19.72 45.09 23.01 15.49
hsa-miR-108 0.00 0.00 0.00 0.00 0.00
hsa-miR-122a 0.00 0.00 0.00 0.00 0.00
hsa-miR-124a 0.00 0.00 0.00 0.00 0.00
hsa-miR-125a 9683.18 4804.78 20089.22 10392.15 2211.89
hsa-miR-125b 762.80 2230.30 995.28 709.73 592.19
hsa-miR-126 2165.59 128.81 50.44 2911.92 1066.11
hsa-miR-126* 378.33 37.03 8.55 321.54 240.08
Continued
268
Table C.2. continued
hsa-miR-127 0.00 2.16 0.00 0.00 3.18
hsa-miR-128a 0.00 0.00 0.00 0.00 0.00
hsa-miR-130a 1216.93 549.23 4377.06 926.57 403.38
hsa-miR-130aN 2618.26 1817.11 9037.45 2229.60 1165.90
hsa-miR-130b 498.16 833.29 1762.70 903.80 404.27
hsa-miR-132 906.37 606.72 821.18 772.87 1412.52
hsa-miR-133a 5.31 4.40 0.00 11.77 0.00
hsa-miR-134 3.30 32.36 2.38 3.98 2.43
hsa-miR-135a 128.17 67.08 41.32 60.66 139.43
hsa-miR-135b 4713.59 1646.60 1155.04 1361.54 1226.12
hsa-miR-138 3.96 15.74 11.60 13.30 33.44
hsa-miR-139 12.97 0.00 2.81 0.00 0.00
hsa-miR-140 572.76 532.56 2018.01 606.24 616.28
hsa-miR-141 1745.93 1128.41 6705.89 609.18 1476.14
hsa-miR-142-3p 5579.99 0.00 12.20 1205.81 11.78
hsa-miR-142-5p 710.77 6.20 4.28 417.58 6.37
hsa-miR-143 0.00 0.00 0.00 0.00 0.00
hsa-miR-145 27.71 6.78 10.18 104.36 22.46
hsa-miR-146 3137.52 728.77 373.72 7566.56 440.81
hsa-miR-146b 734.41 96.44 432.17 2268.08 263.53
hsa-miR-147 0.00 0.00 0.00 0.00 0.00
hsa-miR-148a 673.64 543.52 382.81 1209.71 137.08
hsa-miR-148b 132.87 227.23 427.84 172.71 158.73
hsa-miR-149 1794.34 1177.33 4151.14 2864.00 902.33
hsa-miR-150 21.57 7.81 6.80 50.90 85.35
hsa-miR-152 1661.66 407.09 2605.15 2011.11 1903.20
hsa-miR-152N 1643.86 450.69 2510.97 1376.50 1657.79
hsa-miR-153 0.00 0.00 0.00 0.00 0.00
hsa-miR-154 0.00 0.00 0.00 0.00 0.00
hsa-miR-154* 0.00 0.00 0.00 0.00 0.00
hsa-miR-155 35.00 594.63 84.69 9.31 493.08
hsa-miR-181a 244.18 658.06 856.84 62.33 0.00
hsa-miR-181b 728.23 1607.38 2142.98 284.71 34.95
hsa-miR-181c 10.12 36.29 60.55 10.54 6.02
hsa-miR-182* 66.01 15.12 20.22 49.02 18.76
hsa-miR-183 1389.40 476.98 1006.88 585.86 577.57
hsa-miR-184 278.97 0.00 0.00 348.18 93.44
hsa-miR-186 2295.89 3763.10 7386.77 3147.66 1411.05
hsa-miR-187 0.00 20.06 16.89 2.41 0.00
hsa-miR-188 35.33 41.00 65.72 57.01 28.54
hsa-miR-190 0.00 0.00 0.00 0.00 0.00
Continued
269
Table C.2. continued
hsa-miR-191 5488.01 5080.40 6459.18 7471.67 4164.59
hsa-miR-192 527.15 279.89 447.68 524.59 39.62
hsa-miR-193 366.97 18.44 24.30 385.56 126.24
hsa-miR-194 183.01 55.69 161.45 191.21 21.31
hsa-miR-195 734.89 434.85 984.56 917.31 483.57
hsa-miR-196a 1243.07 992.28 1331.75 2450.78 234.01
hsa-miR-196b 393.29 30.67 17.39 174.89 81.36
hsa-miR-197 1214.73 1423.20 3609.89 2525.99 1382.43
hsa-miR-199a 0.00 0.00 0.00 0.00 0.00
hsa-miR-199a* 0.00 0.00 9.32 0.00 0.00
hsa-miR-199b 0.00 0.00 0.00 0.00 0.00
hsa-miR-199-s 0.00 0.00 0.00 0.00 0.00
hsa-miR-200a 5893.33 4203.88 7628.01 2805.06 2977.62
hsa-miR-200b 10716.71 7665.11 10488.52 10176.51 6113.98
hsa-miR-200bN 4480.53 3348.50 5424.39 3274.34 2516.38
hsa-miR-200c 18969.64 21459.45 86582.01 42338.19 26097.67
hsa-miR-200cN 27278.85 32710.20 84005.63 45245.42 26028.66
hsa-miR-203 3697.47 4213.54 5027.72 10178.17 6553.21
hsa-miR-204 0.00 0.00 4.51 0.00 0.00
hsa-miR-205 19002.31 9310.67 18948.25 12564.18 20304.15
hsa-miR-206 0.00 2.80 3.30 2.15 0.00
hsa-miR-208 0.00 0.00 0.00 0.00 0.00
hsa-miR-210 1746.62 3945.62 3858.69 4218.64 1330.00
hsa-miR-210N 2746.80 5989.76 7487.66 6202.59 2369.62
hsa-miR-211 2.94 0.00 0.00 0.00 0.00
hsa-miR-212 202.25 106.14 194.80 116.10 327.78
hsa-miR-213 0.00 59.56 6.06 0.00 0.00
hsa-miR-214 9.33 9.48 5.72 5.48 20.33
hsa-miR-215 4.50 8.91 9.30 0.00 12.19
hsa-miR-216 0.00 0.00 0.00 0.00 0.00
hsa-miR-217 3.36 0.00 0.00 0.00 0.00
hsa-miR-218 0.00 527.51 17.45 175.11 107.60
hsa-miR-219 0.00 0.00 0.00 0.00 0.00
hsa-miR-220 0.00 0.00 0.00 0.00 0.00
hsa-miR-221 5263.74 2644.30 3883.90 4228.78 4612.91
hsa-miR-222 40414.60 17046.69 17770.14 29584.67 29111.49
hsa-miR-223 17.18 35.90 36.24 12.18 1.95
hsa-miR-296 397.27 2.30 46.66 416.68 2.45
hsa-miR-299 0.00 0.00 0.00 0.00 0.00
hsa-miR-301 965.36 423.55 5180.45 653.93 959.68
hsa-miR-302a 0.00 0.00 0.00 0.00 0.00
Continued
270
Table C.2. continued
hsa-miR-302a* 0.00 0.00 0.00 0.00 0.00
hsa-miR-302b 35.45 56.31 165.84 38.44 24.41
hsa-miR-302c 0.00 20.78 0.00 0.00 14.19
hsa-miR-302c* 0.00 0.00 0.00 0.00 0.00
hsa-miR-302d 3.69 0.00 0.00 0.00 11.90
hsa-miR-320 2693.86 3096.55 4396.66 3073.21 6590.27
hsa-miR-321 13650.16 7422.58 9823.46 4382.17 35515.52
hsa-miR-323 0.00 6.18 0.00 0.00 0.00
hsa-miR-324-3p 623.90 1325.04 2274.23 967.31 896.19
hsa-miR-324-5p 451.43 713.85 3064.81 586.92 657.07
hsa-miR-325 0.00 0.00 0.00 0.00 1.86
hsa-miR-326 0.00 6.14 0.00 7.37 1.91
hsa-miR-328 149.23 222.98 1325.51 292.55 159.77
hsa-miR-330 78.28 26.31 63.57 106.26 34.71
hsa-miR-331 2896.78 3477.73 6992.40 2849.81 2086.21
hsa-miR-335 678.78 53.76 150.98 216.08 264.45
hsa-miR-337 0.00 0.00 0.00 0.00 0.00
hsa-miR-338 17.60 0.00 0.00 20.20 0.00
hsa-miR-339 110.31 115.24 139.68 158.82 108.72
hsa-miR-340 85.94 89.40 169.57 147.71 36.48
hsa-miR-342 454.61 1204.99 1399.69 3457.64 1015.11
hsa-miR-345 656.94 1156.85 1468.44 1005.66 937.58
hsa-miR-346 6.19 6.25 10.59 3.84 35.88
hsa-miR-361 774.16 360.47 868.05 381.60 390.53
hsa-miR-367 0.00 0.00 0.00 0.00 0.00
hsa-miR-368 0.00 0.00 0.00 0.00 0.00
hsa-miR-369 0.00 0.00 0.00 0.00 0.00
hsa-miR-370 100.75 73.24 107.84 123.66 105.54
hsa-miR-371 0.00 1.39 0.00 0.00 0.00
hsa-miR-372 179.95 185.15 139.27 141.50 285.58
hsa-miR-373 0.00 1.84 12.02 0.00 0.00
hsa-miR-374 1869.42 3226.98 5827.30 1695.29 2458.98
hsa-miR-375 262.59 74.29 59.06 228.98 29.78
hsa-miR-376a 3.96 112.36 10.29 9.48 0.00
hsa-miR-377 2.50 1.59 0.00 0.00 0.00
hsa-miR-378 81.93 59.30 35.74 110.11 35.37
hsa-miR-379 0.00 35.06 3.41 4.17 3.36
hsa-miR-380-3P 0.00 0.00 0.00 0.00 0.00
hsa-miR-380-5p 0.00 0.00 0.00 0.00 0.00
hsa-miR-381 0.00 0.00 15.44 0.00 5.61
hsa-miR-382 0.00 46.85 0.00 0.00 2.00
Continued
271
Table C.2. continued
hsa-miR-383 0.00 0.00 0.00 0.00 3.95
hsa-miR-384 0.00 0.00 0.00 0.00 0.00
hsa-miR-422b 81.73 12.21 44.78 68.83 33.34
hsa-miR-423 391.07 727.64 1015.64 597.22 313.14
hsa-miR-425 277.88 139.92 467.20 245.33 171.92
let-7f(mmu) 0.00 0.00 0.00 0.00 0.00
miR-129-5p(mmu) 0.00 0.00 0.00 0.00 0.00
miR-137(mmu) 11.54 0.00 0.00 0.00 24.52
miR-224(mmu) 0.00 0.00 0.00 12.96 195.87
miR-424(mmu) 0.00 0.00 0.00 0.00 0.00
Breast Cancer
miRNA T47D SKBR3 MDA361 MCF7 MDA231
hsa-let-7a 1498.74 870.57 401.65 2045.14 648.65
hsa-let-7b 800.13 5424.95 3941.58 2922.04 246.17
hsa-let-7c 41.34 849.64 985.18 255.92 44.16
hsa-let-7d 0.00 108.15 84.79 317.25 0.00
hsa-let-7e 80.01 83.86 198.59 345.80 0.00
hsa-let-7g 153.64 642.89 459.41 1451.71 40.60
hsa-let-7i 58.12 150.71 73.43 988.90 66.23
hsa-miR-001 0.00 0.00 0.00 0.00 0.00
hsa-miR-007 192.74 169.83 42.46 1454.10 36.79
hsa-miR-009 52.04 567.34 16.49 635.36 19.44
hsa-miR-009* 6.09 52.86 9.64 128.30 0.00
hsa-miR-010a 580.48 0.00 38.64 0.00 101.22
hsa-miR-010b 9.16 0.00 0.00 0.00 0.00
hsa-miR-015a 757.25 159.85 129.69 761.34 32.44
hsa-miR-015b 11503.50 3795.32 7597.36 6613.15 245.00
hsa-miR-016 50925.02 19355.99 21337.19 49759.77 459.92
hsa-miR-017-3p 16.00 4.15 11.53 23.13 0.00
hsa-miR-017-5p 1346.56 772.00 287.62 395.16 70.06
hsa-miR-018 140.41 213.76 71.66 113.81 15.99
hsa-miR-019a 2442.86 3124.30 383.55 1777.67 143.49
hsa-miR-019b 11108.91 13834.15 2470.18 9249.32 817.59
hsa-miR-020 12836.43 13155.39 4057.42 13266.80 0.00
hsa-miR-021 2193.41 1127.54 2291.07 59172.65 89.45
hsa-miR-022 81.96 130.00 115.73 172.28 1.42
hsa-miR-023a 41.93 53.54 56.24 101.85 0.00
hsa-miR-023b 490.04 224.44 510.34 634.33 0.00
hsa-miR-023bN 8771.84 2545.64 3509.36 4124.28 142.33
hsa-miR-024 21049.93 18641.43 24760.84 35646.73 1696.98
Continued
272
Table C.2. continued
hsa-miR-025 1066.59 1612.10 932.12 2989.40 174.42
hsa-miR-026aN 9812.92 3722.20 6215.36 14042.07 7254.63
hsa-miR-026b 3262.33 1513.86 1026.98 5462.80 45.09
hsa-miR-026bN 1757.11 884.11 588.70 3499.17 8.04
hsa-miR-027a 1551.66 1942.48 1037.34 3529.82 356.05
hsa-miR-027b 1867.36 717.08 293.92 1795.35 497.96
hsa-miR-027bN 9042.91 5962.23 3991.23 16414.79 1350.30
hsa-miR-028 344.29 274.84 461.54 507.43 39.61
hsa-miR-029a 321.57 993.01 246.18 355.78 266.59
hsa-miR-029b 58.18 44.49 17.67 36.38 10.11
hsa-miR-029c 584.63 587.96 114.50 374.06 8.63
hsa-miR-030a-3p 621.79 366.05 1409.36 138.52 161.50
hsa-miR-030c 6600.76 5742.83 9952.22 6981.41 1161.27
hsa-miR-030d 487.78 744.02 3694.70 2602.19 50.74
hsa-miR-030e-3p 556.03 726.45 370.48 444.66 32.28
hsa-miR-031 14.40 3050.21 691.74 10.32 376.38
hsa-miR-032 535.07 102.56 36.76 553.70 18.03
hsa-miR-033 6.44 0.00 0.00 0.00 0.00
hsa-miR-034a 55.67 36.31 37.94 127.03 0.00
hsa-miR-034aN 944.76 615.06 387.65 749.21 1.84
hsa-miR-034b 0.00 0.00 0.00 0.00 0.00
hsa-miR-034b 0.00 0.00 0.00 0.00 0.00
hsa-miR-092N 6523.59 9185.64 7370.18 8570.93 56.70
hsa-miR-093 14289.92 14076.43 13635.59 29148.70 0.00
hsa-miR-095 15.40 0.00 106.09 636.75 0.00
hsa-miR-096 12229.11 11180.21 14572.08 16948.46 158.78
hsa-miR-098 22.66 229.72 126.74 937.27 641.10
hsa-miR-099a 33.06 545.66 954.38 130.01 760.15
hsa-miR-099b 1894.27 826.25 5065.86 3324.39 93.83
hsa-miR-100 22.08 326.86 570.26 83.85 874.67
hsa-miR-101 69.45 14.05 18.00 148.07 0.00
hsa-miR-103 1930.92 521.98 944.27 1737.23 11.89
hsa-miR-105 13.27 5.84 0.00 0.00 17.22
hsa-miR-106a 1775.25 1345.88 704.82 1511.32 19.76
hsa-miR-106b 2922.91 2634.66 958.85 5376.53 77.73
hsa-miR-107 41.28 11.82 9.80 34.00 0.00
hsa-miR-108 0.00 0.00 0.00 0.00 0.00
hsa-miR-122a 0.00 0.00 0.00 0.00 0.00
hsa-miR-124a 0.00 0.00 0.00 0.00 0.00
hsa-miR-125a 10610.36 5907.63 34298.11 21630.77 1109.70
hsa-miR-125b 33.88 754.41 1355.85 183.70 1162.40
Continued
273
Table C.2. continued
hsa-miR-126 296.52 115.01 652.26 1483.07 0.00
hsa-miR-126* 94.77 49.87 81.12 259.14 0.00
hsa-miR-127 0.00 0.00 380.84 0.00 0.00
hsa-miR-128a 0.00 0.00 0.00 0.00 0.00
hsa-miR-130a 1667.75 552.09 152.62 2.98 1.93
hsa-miR-130aN 3782.08 1669.05 561.97 4.77 23.25
hsa-miR-130b 534.90 338.02 411.64 393.53 14.85
hsa-miR-132 409.01 117.11 344.49 1003.90 172.12
hsa-miR-133a 0.00 0.00 0.00 0.00 0.00
hsa-miR-134 1.48 3.42 15.09 7.82 11.48
hsa-miR-135a 202.54 0.00 10.98 1079.76 8.87
hsa-miR-135b 37.65 44.11 119.70 311.68 34.75
hsa-miR-138 0.00 2.26 1.77 3.85 7.95
hsa-miR-139 0.00 0.00 0.00 13.33 0.00
hsa-miR-140 555.92 546.61 256.60 857.85 244.23
hsa-miR-141 625.98 1938.85 1032.20 3096.63 0.00
hsa-miR-142-3p 3.33 0.00 0.00 7.90 0.00
hsa-miR-142-5p 5.84 1.69 4.69 4.41 2.78
hsa-miR-143 0.00 0.00 0.00 12.89 0.00
hsa-miR-145 0.00 14.79 21.02 19.61 9.36
hsa-miR-146 405.51 333.55 380.21 311.33 826.08
hsa-miR-146b 289.02 121.45 57.52 242.47 66.87
hsa-miR-147 0.00 0.00 0.00 0.00 0.00
hsa-miR-148a 871.04 544.88 688.91 395.60 0.00
hsa-miR-148b 612.02 127.35 233.78 356.35 4.82
hsa-miR-149 1990.84 1286.17 3107.17 1435.43 76.43
hsa-miR-150 20.77 0.00 10.39 24.03 0.00
hsa-miR-152 704.23 13.14 126.31 464.24 45.17
hsa-miR-152N 749.04 59.42 157.93 550.59 68.70
hsa-miR-153 0.00 0.00 0.00 0.00 0.00
hsa-miR-154 0.00 0.00 0.00 0.00 0.00
hsa-miR-154* 0.00 0.00 0.00 0.00 0.00
hsa-miR-155 15.46 11.26 0.00 17.81 53.85
hsa-miR-181a 934.44 819.13 1631.80 656.42 20.37
hsa-miR-181b 2442.65 2895.16 2575.19 2071.75 199.51
hsa-miR-181c 63.46 39.55 80.90 43.53 2.84
hsa-miR-182* 27.25 29.12 24.42 27.31 3.14
hsa-miR-183 670.02 496.59 927.99 1218.41 11.74
hsa-miR-184 97.66 118.77 100.52 31.45 0.00
hsa-miR-186 2931.40 3726.12 1629.45 1783.62 304.48
hsa-miR-187 204.74 0.00 172.97 12.78 0.00
Continued
274
Table C.2. continued
hsa-miR-188 44.57 50.33 34.74 29.62 42.15
hsa-miR-190 0.00 0.00 0.00 0.00 0.00
hsa-miR-191 10861.30 7001.03 27487.01 8542.76 460.62
hsa-miR-192 848.53 175.86 302.99 694.20 57.61
hsa-miR-193 647.36 324.80 420.16 661.17 22.20
hsa-miR-194 203.98 29.93 169.07 241.10 12.49
hsa-miR-195 934.69 359.98 228.29 2533.98 30.24
hsa-miR-196a 11284.40 807.33 1718.36 122.02 32.91
hsa-miR-196b 2.11 0.00 0.00 0.00 0.00
hsa-miR-197 1618.67 1485.51 3025.34 1677.30 476.30
hsa-miR-199a 0.00 0.00 0.00 0.00 0.00
hsa-miR-199a* 16.86 11.00 0.00 9.21 0.00
hsa-miR-199b 0.00 0.00 0.00 0.00 0.00
hsa-miR-199-s 0.00 0.00 0.00 0.00 0.00
hsa-miR-200a 4873.52 10.30 1966.60 5197.61 0.00
hsa-miR-200b 8457.67 4.90 7128.69 7749.47 0.00
hsa-miR-200bN 3880.92 103.03 5200.34 5423.04 9.62
hsa-miR-200c 13019.23 32384.49 88921.49 32197.20 0.00
hsa-miR-200cN 11492.03 29978.08 70071.61 29302.55 4.20
hsa-miR-203 8507.06 459.16 2229.81 10460.21 0.00
hsa-miR-204 0.00 0.00 0.00 0.00 24.60
hsa-miR-205 1801.77 93.86 6731.92 52.70 0.00
hsa-miR-206 4.53 0.00 12.27 0.00 0.00
hsa-miR-208 0.00 0.00 0.00 0.00 0.00
hsa-miR-210 942.76 589.61 1599.54 505.32 71.07
hsa-miR-210N 1533.71 869.99 2284.77 958.25 142.42
hsa-miR-211 0.00 0.00 0.00 22.34 0.00
hsa-miR-212 154.95 120.80 222.56 236.86 140.39
hsa-miR-213 12.18 126.10 56.35 5.03 4.21
hsa-miR-214 12.83 12.51 3.89 9.47 5.24
hsa-miR-215 11.86 0.00 11.79 0.00 0.00
hsa-miR-216 0.00 0.00 0.00 0.00 0.00
hsa-miR-217 0.00 0.00 0.00 0.00 0.00
hsa-miR-218 9.63 0.00 0.00 277.06 0.00
hsa-miR-219 0.00 0.00 0.00 0.00 0.00
hsa-miR-220 0.00 0.00 0.00 0.00 0.00
hsa-miR-221 0.00 2.48 0.00 139.68 201.49
hsa-miR-222 6.59 425.70 98.30 1154.48 5827.27
hsa-miR-223 0.00 5.26 1.58 44.65 0.00
hsa-miR-296 757.33 522.24 34.03 1738.66 386.05
hsa-miR-299 0.00 0.00 0.00 0.00 0.00
Continued
275
Table C.2. continued
hsa-miR-301 4943.87 218.46 1209.26 8225.62 22.28
hsa-miR-302a 0.00 0.00 0.00 0.00 0.00
hsa-miR-302a* 0.00 0.00 0.00 0.00 0.00
hsa-miR-302b 42.02 41.53 16.65 30.49 53.60
hsa-miR-302c 0.00 61.26 0.00 0.00 0.00
hsa-miR-302c* 0.00 0.00 0.00 0.00 0.00
hsa-miR-302d 0.00 0.00 1.56 10.03 0.00
hsa-miR-320 4970.52 1722.84 2372.43 3364.47 928.48
hsa-miR-321 5745.97 5749.84 17712.84 9302.29 1408.56
hsa-miR-323 0.00 0.00 2.02 0.00 0.00
hsa-miR-324-3p 1271.33 114.67 1223.51 1176.52 139.26
hsa-miR-324-5p 1205.83 55.49 628.77 1225.76 55.51
hsa-miR-325 0.00 0.00 0.00 0.00 0.00
hsa-miR-326 15.03 0.00 7.00 22.72 0.00
hsa-miR-328 991.98 30.03 1468.84 347.30 49.42
hsa-miR-330 32.76 5.43 40.30 41.67 0.00
hsa-miR-331 3125.21 2410.47 13185.67 2953.99 56.15
hsa-miR-335 0.00 512.36 47.39 399.00 0.00
hsa-miR-337 0.00 0.00 0.00 0.00 0.00
hsa-miR-338 0.00 0.00 0.00 0.00 0.00
hsa-miR-339 133.91 94.38 172.69 214.64 5.18
hsa-miR-340 201.75 76.90 128.21 206.35 15.24
hsa-miR-342 2258.91 794.08 1871.69 20000.33 121.53
hsa-miR-345 1429.28 611.30 2285.66 4000.28 29.30
hsa-miR-346 8.37 8.01 16.52 49.75 1.89
hsa-miR-361 191.93 95.40 88.81 249.58 16.46
hsa-miR-367 0.00 0.00 0.00 0.00 0.00
hsa-miR-368 0.00 0.00 0.00 0.00 0.00
hsa-miR-369 0.00 0.00 0.00 0.00 0.00
hsa-miR-370 67.96 79.56 173.90 138.53 60.16
hsa-miR-371 0.00 0.00 0.00 0.00 0.00
hsa-miR-372 223.30 117.62 127.50 231.01 68.32
hsa-miR-373 0.00 0.00 0.00 0.00 4.47
hsa-miR-374 3029.43 2150.67 803.59 3746.70 11.82
hsa-miR-375 2889.81 470.68 21466.35 1474.46 4.85
hsa-miR-376a 0.00 1.45 18.66 0.00 0.00
hsa-miR-377 0.00 0.00 0.00 0.00 0.00
hsa-miR-378 20.59 57.74 81.89 36.93 23.44
hsa-miR-379 0.00 2.68 21.74 0.00 0.00
hsa-miR-380-3P 0.00 0.00 0.00 0.00 0.00
hsa-miR-380-5p 0.00 0.00 0.00 0.00 0.00
Continued
276
Table C.2. continued
hsa-miR-381 0.00 0.00 4.90 0.00 17.70
hsa-miR-382 0.00 0.00 11.98 0.00 0.00
hsa-miR-383 0.00 0.00 0.00 16.39 0.00
hsa-miR-384 0.00 0.00 0.00 0.00 0.00
hsa-miR-422b 29.84 108.27 28.79 47.56 25.81
hsa-miR-423 708.68 590.66 1283.90 416.90 95.42
hsa-miR-425 704.22 241.59 943.45 421.79 29.72
let-7f(mmu) 0.00 0.00 0.00 0.00 0.00
miR-129-5p(mmu) 0.00 0.00 0.00 0.00 0.00
miR-137(mmu) 0.00 0.00 0.00 0.00 0.00
miR-224(mmu) 0.00 91.37 0.00 0.00 0.00
miR-424(mmu) 0.00 0.00 0.00 0.00 0.00
Colorectal Cancer
miRNA HCT8 HCT116 HT29 SW620 HCT15 HeLa
hsa-let-7a 2981.59 183.91 1458.70 1146.77 1994.70 97.64
hsa-let-7b 14540.76 679.83 4177.99 4045.54 18455.59 3004.33
hsa-let-7c 650.74 34.35 237.37 297.57 733.22 347.79
hsa-let-7d 130.88 13.11 237.52 131.11 84.33 9.31
hsa-let-7e 226.88 7.58 247.92 45.25 151.88 5.55
hsa-let-7g 1671.78 136.22 929.23 1390.01 2045.86 23.38
hsa-let-7i 183.71 102.34 429.32 279.31 257.20 164.52
hsa-miR-001 13.65 0.00 0.00 49.13 0.00 0.00
hsa-miR-007 302.92 124.84 2469.58 3391.31 955.96 0.00
hsa-miR-009 31.67 254.87 5.36 3332.02 15.83 0.00
hsa-miR-009* 18.39 45.33 0.00 594.67 0.00 1.37
hsa-miR-010a 11539.35 231.93 6261.34 3321.67 5038.28 0.00
hsa-miR-010b 3273.00 4.93 1815.59 1343.14 435.07 0.00
hsa-miR-015a 387.64 0.00 441.85 493.21 694.29 17.46
hsa-miR-015b 4008.22 394.29 5725.16 6057.67 4583.43 493.58
hsa-miR-016 29240.66 2019.13 22778.48 36400.59 31816.51 57.90
hsa-miR-017-3p 36.34 0.00 42.81 16.83 11.80 0.00
hsa-miR-017-5p 2861.47 279.37 2015.85 4931.14 3440.33 27.67
hsa-miR-018 281.96 43.23 798.21 461.67 357.08 1.50
hsa-miR-019a 5080.70 812.14 8003.93 14387.34 7672.47 0.00
hsa-miR-019b 25484.30 3165.02 34546.51 66636.93 37604.17 22.80
hsa-miR-020 34241.85 4357.09 44883.87 80463.19 50806.43 161.35
hsa-miR-021 1676.77 1527.42 4011.47 3527.59 4460.74 22.00
hsa-miR-022 66.12 0.00 117.41 9.13 81.13 0.00
hsa-miR-023a 18.71 11.27 32.92 55.58 118.78 8.98
hsa-miR-023b 132.36 30.39 190.24 422.68 68.79 0.00
Continued
277
Table C.2. continued
hsa-miR-023bN 1765.34 247.55 2098.77 11575.28 1745.85 233.63
hsa-miR-024 12999.89 2529.96 26074.78 36341.93 28448.54 1250.05
hsa-miR-025 963.37 419.84 2070.35 3664.79 1113.67 35.63
hsa-miR-026aN 10395.19 925.23 4996.18 9947.63 6718.07 79.77
hsa-miR-026b 3304.79 229.07 3109.24 3602.51 4491.24 20.15
hsa-miR-026bN 1605.33 119.74 1635.25 1952.60 2239.18 10.79
hsa-miR-027a 1372.15 529.44 1644.03 2344.51 4434.74 46.14
hsa-miR-027b 531.21 20.88 486.81 2608.71 409.93 17.39
hsa-miR-027bN 3220.54 160.89 3748.76 11881.83 1981.29 169.79
hsa-miR-028 305.87 48.73 642.32 440.15 315.05 0.00
hsa-miR-029a 3957.26 809.99 1976.32 3391.55 3048.37 20.87
hsa-miR-029b 170.26 36.07 85.41 96.66 159.85 0.00
hsa-miR-029c 293.55 88.99 229.72 204.47 294.84 0.00
hsa-miR-030a-3p 0.00 0.00 0.00 39.94 108.86 108.19
hsa-miR-030c 6404.44 1147.38 5324.90 6262.14 4542.52 123.32
hsa-miR-030d 674.24 107.79 2021.45 633.55 271.64 10.93
hsa-miR-030e-3p 750.43 46.13 311.19 833.51 546.77 3.63
hsa-miR-031 3214.75 17316.78 48373.34 3645.68 8322.72 340.26
hsa-miR-032 208.14 40.98 153.82 152.39 276.81 0.00
hsa-miR-033 0.00 0.00 0.00 0.00 0.00 0.00
hsa-miR-034a 62.82 0.00 12.82 4.08 19.58 19.38
hsa-miR-034aN 753.46 156.77 186.64 101.05 280.87 65.99
hsa-miR-034b 0.00 0.00 0.00 0.00 0.00 0.00
hsa-miR-034b 0.00 0.00 0.00 0.00 0.00 0.00
hsa-miR-092N 18967.69 2405.82 23341.01 54271.90 21328.96 4638.85
hsa-miR-093 16206.79 2448.54 23278.79 34278.99 17925.49 751.21
hsa-miR-095 346.78 75.88 22.14 391.30 136.75 24.34
hsa-miR-096 17398.44 12454.29 10421.57 11006.18 37633.61 48346.58
hsa-miR-098 216.04 29.24 492.65 323.15 348.71 0.00
hsa-miR-099a 198.10 2012.21 106.30 1461.20 92.11 59.50
hsa-miR-099b 825.85 302.29 2126.07 559.74 1524.52 42.66
hsa-miR-100 158.95 2342.43 118.76 1604.34 54.61 25.35
hsa-miR-101 38.32 9.03 62.66 81.59 56.34 0.00
hsa-miR-103 550.54 147.47 1362.73 1288.98 1002.92 10.91
hsa-miR-105 0.00 0.00 0.00 0.00 2.77 0.00
hsa-miR-106a 3683.00 995.85 2439.15 4614.12 5110.52 2901.07
hsa-miR-106b 1880.20 449.59 3230.59 3480.99 2940.59 18.04
hsa-miR-107 20.30 0.00 16.55 42.79 0.00 0.00
hsa-miR-108 0.00 0.00 0.00 0.00 0.00 0.00
hsa-miR-122a 0.00 0.00 0.00 0.00 0.00 0.00
hsa-miR-124a 0.00 0.00 0.00 0.00 0.00 0.00
Continued
278
Table C.2. continued
hsa-miR-125a 8212.77 1859.42 10620.96 4116.32 12708.71 887.81
hsa-miR-125b 0.00 2224.88 20.36 831.65 29.05 227.86
hsa-miR-126 938.73 314.39 37.38 1098.62 5.78 0.00
hsa-miR-126* 636.12 234.70 78.21 751.68 3.53 8.09
hsa-miR-127 0.00 0.00 0.00 0.00 0.00 0.00
hsa-miR-128a 0.00 0.00 0.00 0.00 0.00 0.00
hsa-miR-130a 164.04 81.53 101.90 258.04 8.58 2.76
hsa-miR-130aN 18.92 150.94 21.83 60.03 2.17 26.95
hsa-miR-130b 817.38 148.94 473.50 1081.93 968.71 11.27
hsa-miR-132 441.82 142.27 307.82 1863.09 651.60 70.17
hsa-miR-133a 4.58 0.00 0.00 25.14 0.00 8.48
hsa-miR-134 9.65 3.84 14.04 9.82 7.06 8.29
hsa-miR-135a 28.33 4.93 46.85 494.73 9.42 0.00
hsa-miR-135b 771.14 295.36 1244.98 2450.92 226.45 0.00
hsa-miR-138 0.00 0.00 10.86 4.83 1.93 0.00
hsa-miR-139 12.36 4.08 0.00 0.00 0.00 0.00
hsa-miR-140 1611.25 170.68 696.76 2626.31 2319.61 0.00
hsa-miR-141 1232.71 338.65 1274.09 26.59 1291.73 0.00
hsa-miR-142-3p 227.84 0.00 198.21 5492.13 77.41 0.00
hsa-miR-142-5p 58.04 7.35 49.78 1692.60 15.67 2.71
hsa-miR-143 3.16 0.00 0.00 2.19 0.00 0.00
hsa-miR-145 6.95 28.55 7.99 20.89 6.55 6.57
hsa-miR-146 462.04 302.07 885.46 665.53 392.93 349.86
hsa-miR-146b 2911.73 90.27 143.77 500.11 293.38 17.26
hsa-miR-147 0.00 0.00 0.00 0.00 0.00 0.00
hsa-miR-148a 792.39 94.73 69.32 926.29 1169.72 0.00
hsa-miR-148b 223.71 49.20 232.68 286.83 245.40 0.00
hsa-miR-149 330.27 267.94 177.26 1177.31 704.51 32.52
hsa-miR-150 7.95 22.21 350.76 232.74 23.03 17.93
hsa-miR-152 490.61 19.04 164.36 685.59 565.02 32.53
hsa-miR-152N 512.91 62.09 220.70 442.64 474.89 26.82
hsa-miR-153 0.00 0.00 0.00 0.00 0.00 0.00
hsa-miR-154 0.00 0.00 0.00 0.00 0.00 0.00
hsa-miR-154* 0.00 0.00 0.00 0.00 0.00 0.00
hsa-miR-155 36.25 2.38 146.31 7.51 552.12 52.39
hsa-miR-181a 4.37 29.48 564.03 663.50 58.26 115.06
hsa-miR-181b 115.39 153.53 1929.59 2033.60 256.72 223.55
hsa-miR-181c 1.99 4.03 37.81 76.67 17.59 7.03
hsa-miR-182* 19.92 13.41 24.77 16.20 25.62 12.38
hsa-miR-183 427.97 97.01 419.13 914.00 627.03 2.10
hsa-miR-184 0.00 1.48 0.00 0.00 14.35 0.00
Continued
279
Table C.2. continued
hsa-miR-186 1822.96 861.05 5015.29 3939.76 3434.66 21.57
hsa-miR-187 0.00 0.00 0.00 0.00 0.00 0.00
hsa-miR-188 18.45 38.05 47.61 51.40 30.78 32.45
hsa-miR-190 0.00 0.00 0.00 0.00 0.00 0.00
hsa-miR-191 3821.39 1563.03 3664.16 5584.09 10469.55 291.06
hsa-miR-192 4407.85 22.58 24270.80 14008.19 933.89 0.00
hsa-miR-193 178.07 26.59 42.27 6.05 38.45 9.63
hsa-miR-194 1218.36 6.71 5196.53 3638.52 292.06 0.00
hsa-miR-195 299.43 28.62 189.76 261.73 375.13 1.81
hsa-miR-196a 1304.06 134.34 2638.79 3410.42 1153.35 228.79
hsa-miR-196b 0.00 0.00 395.26 265.11 16.98 0.00
hsa-miR-197 1241.78 1254.49 1769.50 1904.93 1980.97 292.87
hsa-miR-199a 0.00 0.00 0.00 0.00 0.00 0.00
hsa-miR-199a* 9.67 0.00 6.85 8.74 12.62 0.00
hsa-miR-199b 0.00 0.00 0.00 0.00 0.00 0.00
hsa-miR-199-s 0.00 0.00 0.00 0.00 0.00 0.00
hsa-miR-200a 3803.53 667.47 14210.01 2946.38 4976.87 0.00
hsa-miR-200b 11575.51 599.72 40770.61 10992.02 12333.80 0.00
hsa-miR-200bN 3800.74 278.08 13412.99 3434.33 3696.94 0.00
hsa-miR-200c 26716.43 4957.90 23494.64 1106.80 35698.53 1.46
hsa-miR-200cN 22284.01 3550.53 24454.01 667.17 39036.64 0.00
hsa-miR-203 9763.52 343.04 3009.16 831.61 6205.45 13.62
hsa-miR-204 0.00 0.00 0.00 921.30 48.75 4.78
hsa-miR-205 0.00 0.00 79.10 13.53 0.00 0.00
hsa-miR-206 3.96 2.07 8.99 1.50 0.00 1.43
hsa-miR-208 0.00 0.00 0.00 0.00 0.00 0.00
hsa-miR-210 913.12 116.36 538.88 333.63 858.50 5.70
hsa-miR-210N 1498.52 248.57 713.75 490.69 1558.61 29.03
hsa-miR-211 0.00 0.00 0.00 0.00 0.00 0.00
hsa-miR-212 209.55 186.29 145.47 329.65 203.79 73.62
hsa-miR-213 0.00 2.26 40.38 88.13 2.86 0.00
hsa-miR-214 5.26 6.78 10.12 3.93 0.00 6.06
hsa-miR-215 39.17 0.00 139.59 26.67 15.20 0.00
hsa-miR-216 0.00 0.00 0.00 0.00 0.00 0.00
hsa-miR-217 7.64 0.00 0.00 0.00 1.42 0.00
hsa-miR-218 0.00 19.45 0.00 51.08 1.41 0.00
hsa-miR-219 0.00 0.00 0.00 0.00 0.00 0.00
hsa-miR-220 0.00 0.00 0.00 0.00 0.00 0.00
hsa-miR-221 1607.73 1101.84 3639.45 3074.69 2441.29 9.91
hsa-miR-222 13748.91 14902.59 19900.07 19247.57 20363.61 730.06
hsa-miR-223 0.00 0.00 0.00 0.00 0.00 21.54
Continued
280
Table C.2. continued
hsa-miR-296 18.71 22.47 5.71 5.32 2.79 94.50
hsa-miR-299 0.00 0.00 0.00 0.00 0.00 0.00
hsa-miR-301 642.38 201.45 1388.28 842.44 1467.44 0.00
hsa-miR-302a 0.00 0.00 0.00 0.00 0.00 0.00
hsa-miR-302a* 0.00 0.00 0.00 0.00 0.00 0.00
hsa-miR-302b 7.78 4.95 1.54 6.39 108.25 34.35
hsa-miR-302c 0.00 34.38 0.00 0.00 24.26 0.00
hsa-miR-302c* 0.00 0.00 0.00 0.00 0.00 0.00
hsa-miR-302d 0.00 0.00 0.00 0.00 0.00 0.00
hsa-miR-320 4707.69 1193.35 1854.40 7736.92 6465.03 881.54
hsa-miR-321 6516.35 1613.15 18992.34 8820.76 6827.65 505.53
hsa-miR-323 0.00 0.00 0.00 0.00 0.00 0.00
hsa-miR-324-3p 285.05 127.77 483.67 1513.06 1430.63 122.48
hsa-miR-324-5p 640.68 155.76 449.69 542.14 906.36 8.14
hsa-miR-325 0.00 0.00 0.00 0.00 0.00 0.00
hsa-miR-326 7.15 2.11 5.08 2.21 9.05 0.00
hsa-miR-328 226.63 135.11 59.95 791.33 178.80 46.52
hsa-miR-330 39.71 15.17 56.23 29.63 61.11 1.76
hsa-miR-331 1673.72 778.22 2668.59 2437.66 2157.33 321.60
hsa-miR-335 351.12 21.95 324.67 1093.08 184.04 0.00
hsa-miR-337 0.00 0.00 0.00 0.00 0.00 0.00
hsa-miR-338 0.00 0.00 106.59 0.00 9.19 0.00
hsa-miR-339 83.50 28.73 78.38 108.15 41.52 10.02
hsa-miR-340 124.51 34.50 108.95 180.35 89.00 0.00
hsa-miR-342 0.00 176.07 0.00 13.76 0.00 43.99
hsa-miR-345 723.00 207.45 380.66 765.68 600.24 19.54
hsa-miR-346 10.11 13.85 20.02 33.91 8.20 27.85
hsa-miR-361 242.91 62.41 256.46 596.96 267.64 4.41
hsa-miR-367 0.00 0.00 0.00 0.00 0.00 0.00
hsa-miR-368 0.00 0.00 0.00 0.00 0.00 0.00
hsa-miR-369 0.00 0.00 0.00 0.00 0.00 0.00
hsa-miR-370 92.82 80.39 102.63 44.87 57.94 85.25
hsa-miR-371 106.77 0.00 0.00 0.00 507.15 0.00
hsa-miR-372 602.69 99.64 84.52 264.02 2785.91 129.72
hsa-miR-373 31.48 0.00 0.00 3.39 288.91 0.00
hsa-miR-374 3849.70 417.93 5233.17 6911.48 1880.54 0.00
hsa-miR-375 1217.73 124.35 3433.88 23411.10 4933.26 0.00
hsa-miR-376a 0.00 0.00 5.90 2.29 7.15 0.00
hsa-miR-377 5.68 0.00 1.99 9.73 1.87 0.00
hsa-miR-378 102.18 22.53 74.04 36.02 91.66 12.17
hsa-miR-379 1.77 0.00 2.31 0.00 0.00 0.00
Continued
281
Table C.2. continued
hsa-miR-380-3P 0.00 0.00 0.00 0.00 0.00 0.00
hsa-miR-380-5p 1.53 0.00 0.00 0.00 0.00 0.00
hsa-miR-381 0.00 7.22 0.00 3.45 0.00 0.00
hsa-miR-382 0.00 0.00 0.00 0.00 0.00 0.00
hsa-miR-383 0.00 0.00 0.00 0.00 3.58 0.00
hsa-miR-384 0.00 0.00 0.00 0.00 0.00 0.00
hsa-miR-422b 76.55 26.23 7.23 40.17 44.74 2.56
hsa-miR-423 411.15 194.95 398.49 467.78 470.12 288.90
hsa-miR-425 183.07 55.19 227.40 314.96 759.80 12.15
let-7f(mmu) 0.00 0.00 0.00 0.00 0.00 0.00
miR-129-5p(mmu) 0.00 0.00 0.00 0.00 0.00 0.00
miR-137(mmu) 0.00 0.00 0.00 0.00 0.00 0.00
miR-224(mmu) 8.52 0.00 144.45 7.48 8.60 3.95
miR-424(mmu) 0.00 0.00 0.00 0.00 0.00 0.00
Hematological Cancer
miRNA RAMOS HL60 K562 JURCAT DAUDI 293T
hsa-let-7a 476.24 1227.07 158.91 1561.33 257.93 576.04
hsa-let-7b 170.17 1327.88 18.92 2422.87 180.41 199.06
hsa-let-7c 395.07 149.41 0.00 143.65 95.66 330.75
hsa-let-7d 117.48 168.89 0.00 201.13 70.05 0.00
hsa-let-7e 0.00 5.02 12.18 20.57 0.00 46.84
hsa-let-7g 3023.14 2037.42 46.67 2713.04 1840.27 546.74
hsa-let-7i 334.50 261.92 36.01 479.18 302.21 70.98
hsa-miR-001 0.00 0.00 0.00 4.28 0.00 41.13
hsa-miR-007 231.19 78.48 83.06 2271.12 501.25 871.54
hsa-miR-009 116.83 30.74 833.69 3556.40 432.26 1892.64
hsa-miR-009* 40.55 3.27 272.19 698.75 166.10 468.84
hsa-miR-010a 0.00 0.00 669.55 0.00 5.80 2487.12
hsa-miR-010b 0.00 0.00 13.70 0.00 0.00 749.90
hsa-miR-015a 1160.95 697.23 252.42 1735.77 231.07 783.29
hsa-miR-015b 8136.28 5490.35 6712.70 9037.28 2819.87 6407.23
hsa-miR-016 100287.15 35183.56 27742.35 86180.90 42795.43 43217.17
hsa-miR-017-3p 209.81 78.37 123.85 96.83 176.82 135.05
hsa-miR-017-5p 2391.84 4252.95 8317.35 6578.31 2424.41 8712.46
hsa-miR-018 748.84 601.23 2138.55 1502.74 541.72 1418.87
hsa-miR-019a 15383.65 10144.15 27202.80 17315.74 11819.43 21531.63
hsa-miR-019b 64653.48 57911.03 105820.80 82056.41 63322.49 88132.29
hsa-miR-020 80036.65 70308.31 140864.52 105847.92 68168.51 73186.00
hsa-miR-021 330.54 349.04 4476.93 699.20 682.04 439.93
hsa-miR-022 6.82 0.00 101.16 29.17 7.67 100.80
Continued
282
Table C.2. continued
hsa-miR-023a 0.00 94.37 25.58 4.68 0.00 15.10
hsa-miR-023b 22.05 32.95 23.53 40.98 0.00 28.69
hsa-miR-023bN 150.68 606.89 668.25 933.75 11.35 1156.84
hsa-miR-024 1205.61 16971.70 7051.43 3361.53 269.19 3258.01
hsa-miR-025 2553.96 2558.49 3451.85 3218.04 722.52 2667.26
hsa-miR-026aN 4750.03 3081.36 5040.04 5105.37 1200.29 4822.47
hsa-miR-026b 1480.03 3230.85 2766.01 3245.93 734.25 2257.01
hsa-miR-026bN 829.46 1418.85 1468.50 1428.07 511.42 1128.64
hsa-miR-027a 54.02 3629.05 1092.28 552.12 34.25 432.68
hsa-miR-027b 0.00 84.69 127.77 206.60 14.87 355.65
hsa-miR-027bN 1482.78 562.68 476.28 1373.40 279.75 2299.56
hsa-miR-028 201.68 145.03 0.00 647.47 442.10 227.69
hsa-miR-029a 2085.19 525.96 343.58 1043.35 1192.86 422.56
hsa-miR-029b 24.98 32.59 0.00 74.82 24.95 38.42
hsa-miR-029c 276.03 298.80 92.05 2086.16 532.72 272.20
hsa-miR-030a-3p 20.97 0.00 2.36 0.00 0.00 762.23
hsa-miR-030c 5574.80 7402.57 6113.83 9477.76 3176.38 7134.64
hsa-miR-030d 1476.87 561.82 1004.18 832.28 1050.14 587.32
hsa-miR-030e-3p 420.02 861.02 876.56 1050.16 354.99 677.80
hsa-miR-031 1.88 0.00 0.00 8.27 38.13 5053.65
hsa-miR-032 359.92 227.02 487.27 934.25 94.47 598.02
hsa-miR-033 0.00 0.00 0.00 0.00 0.00 0.00
hsa-miR-034a 0.00 0.00 0.00 0.00 0.00 95.07
hsa-miR-034aN 2.24 0.00 11.49 8.76 0.00 939.42
hsa-miR-034b 0.00 0.00 0.00 0.00 0.00 10.87
hsa-miR-034b 0.00 0.00 0.00 0.00 0.00 0.00
hsa-miR-092N 36634.07 38856.53 76696.29 45585.33 29405.83 49278.04
hsa-miR-093 12102.83 22405.08 16757.50 32796.90 2432.46 18029.98
hsa-miR-095 46.43 0.00 0.00 58.53 57.31 228.99
hsa-miR-096 16954.20 10215.68 15049.70 12537.86 14053.04 15450.54
hsa-miR-098 34.67 167.95 0.00 782.04 7.91 68.43
hsa-miR-099a 630.48 158.16 17.61 10.08 379.06 528.84
hsa-miR-099b 0.00 6.74 714.62 134.99 3.75 659.87
hsa-miR-100 255.47 65.90 11.69 7.33 206.41 318.65
hsa-miR-101 101.93 62.14 109.87 84.69 43.61 100.83
hsa-miR-103 406.14 736.39 478.35 1212.18 189.56 1100.61
hsa-miR-105 0.00 0.00 32.45 0.00 8.16 0.00
hsa-miR-106a 2529.12 3257.09 4641.96 5226.03 2456.19 7592.53
hsa-miR-106b 5421.12 3804.48 3161.85 6202.17 1406.73 3724.92
hsa-miR-107 0.00 18.08 14.58 20.62 0.00 31.43
hsa-miR-108 0.00 0.00 0.00 0.00 0.00 0.00
hsa-miR-122a 0.00 0.00 0.00 0.00 0.00 0.00
Continued
283
Table C.2. continued
hsa-miR-124a 0.00 0.00 0.00 0.00 0.00 0.00
hsa-miR-125a 0.00 160.74 5429.93 906.74 22.52 3792.74
hsa-miR-125b 710.54 133.81 0.00 0.00 303.84 850.49
hsa-miR-126 0.00 18.49 15649.06 849.30 0.00 1462.15
hsa-miR-126* 4.89 21.32 3037.39 170.97 0.00 253.11
hsa-miR-127 0.00 0.00 35.34 0.00 0.00 12.45
hsa-miR-128a 0.00 0.00 0.00 0.00 0.00 0.00
hsa-miR-130a 5.15 0.00 54.28 29.04 4.60 27.99
hsa-miR-130aN 20.92 1.52 6.86 46.51 22.27 2.61
hsa-miR-130b 495.88 200.07 2751.96 1735.27 294.28 897.40
hsa-miR-132 79.16 45.56 525.33 724.73 563.68 1058.09
hsa-miR-133a 5.19 5.35 0.00 0.00 0.00 8.31
hsa-miR-134 1.82 3.47 20.45 5.54 4.74 7.27
hsa-miR-135a 0.00 8.35 29.26 174.93 0.00 0.00
hsa-miR-135b 0.00 0.00 241.70 3.28 0.00 58.73
hsa-miR-138 52.05 0.00 0.00 5.76 6.46 13.15
hsa-miR-139 0.00 0.00 0.00 11.35 0.00 7.83
hsa-miR-140 2199.51 2571.88 1993.32 1951.24 828.19 1597.67
hsa-miR-141 0.00 5.81 4.53 49.08 0.00 5.98
hsa-miR-142-3p 19725.56 23664.42 12437.70 40066.70 13900.74 15.09
hsa-miR-142-5p 2571.31 3874.12 1784.88 6533.13 1367.77 4.79
hsa-miR-143 0.00 0.00 0.00 0.00 0.00 0.00
hsa-miR-145 6.10 24.56 51.85 25.44 2.65 6.79
hsa-miR-146 11941.26 1403.02 537.84 892.52 865.85 552.42
hsa-miR-146b 286.39 214.77 17729.83 312.77 308.05 470.55
hsa-miR-147 0.00 0.00 0.00 0.00 0.00 0.00
hsa-miR-148a 1558.38 917.15 1176.32 2484.78 181.01 2497.30
hsa-miR-148b 94.85 188.80 141.11 242.27 33.68 298.74
hsa-miR-149 7.98 5.61 10.98 176.83 19.14 772.47
hsa-miR-150 992.51 23.18 0.00 5179.88 866.32 12.31
hsa-miR-152 199.34 4.35 47.43 127.16 38.36 275.01
hsa-miR-152N 185.95 29.66 40.18 154.50 66.98 369.07
hsa-miR-153 0.00 0.00 0.00 8.89 0.00 0.00
hsa-miR-154 0.00 0.00 0.00 0.00 0.00 0.00
hsa-miR-154* 0.00 0.00 0.00 0.00 0.00 0.00
hsa-miR-155 31.61 434.48 6.13 5.23 337.71 6.95
hsa-miR-181a 1060.60 3933.94 0.00 6586.14 178.40 474.63
hsa-miR-181b 2127.41 6839.53 58.32 17286.88 899.36 938.48
hsa-miR-181c 59.83 163.01 0.00 342.21 15.41 57.96
hsa-miR-182* 2.80 0.00 19.09 22.36 0.00 21.75
hsa-miR-183 82.41 2.48 480.61 687.52 2.16 354.98
hsa-miR-184 0.00 0.00 0.00 86.69 30.59 18.03
Continued
284
Table C.2. continued
hsa-miR-186 1551.68 5441.12 4549.78 6923.61 621.71 7705.67
hsa-miR-187 0.00 0.00 0.00 11.67 0.00 212.58
hsa-miR-188 20.91 27.89 26.29 19.80 21.06 66.72
hsa-miR-190 0.00 0.00 0.00 0.00 0.00 0.00
hsa-miR-191 9349.85 14701.60 8680.03 16887.18 1467.62 5974.20
hsa-miR-192 259.75 70.63 480.52 575.28 389.65 830.94
hsa-miR-193 10.26 25.09 0.00 42.78 17.20 38.91
hsa-miR-194 65.60 26.11 143.87 180.91 132.32 260.42
hsa-miR-195 926.49 299.42 295.38 724.90 336.23 853.43
hsa-miR-196a 0.00 14.47 167.16 954.39 35.22 4465.57
hsa-miR-196b 0.00 0.00 0.00 0.00 0.00 2007.48
hsa-miR-197 638.13 2129.18 2248.85 1245.66 211.17 3281.06
hsa-miR-199a 0.00 0.00 0.00 0.00 0.00 0.00
hsa-miR-199a* 0.00 327.50 23.91 5.54 19.34 11.94
hsa-miR-199b 0.00 35.34 0.00 0.00 0.00 0.00
hsa-miR-199-s 0.00 0.00 0.00 0.00 0.00 0.00
hsa-miR-200a 1.97 0.00 0.00 0.00 0.00 7.08
hsa-miR-200b 7.85 8.27 8.68 9.27 0.00 23.33
hsa-miR-200bN 18.19 0.00 3.20 2.44 0.00 4.80
hsa-miR-200c 19.18 68.93 99.69 606.65 28.55 187.68
hsa-miR-200cN 23.80 57.47 75.76 512.11 0.00 157.62
hsa-miR-203 8.44 0.00 0.00 221.64 0.00 18.43
hsa-miR-204 2.20 0.00 3.87 0.00 0.00 121.80
hsa-miR-205 0.00 0.00 0.00 0.00 0.00 0.00
hsa-miR-206 4.54 0.00 0.00 6.68 3.28 0.00
hsa-miR-208 0.00 0.00 0.00 0.00 0.00 0.00
hsa-miR-210 569.34 7.26 243.67 314.03 96.74 1119.41
hsa-miR-210N 924.90 46.43 462.86 511.37 250.23 1893.90
hsa-miR-211 0.00 0.00 0.00 0.00 0.00 0.00
hsa-miR-212 125.79 79.52 215.32 167.87 114.88 217.90
hsa-miR-213 20.63 601.11 0.00 994.57 14.24 34.27
hsa-miR-214 9.22 2.12 4.80 2.85 19.88 14.66
hsa-miR-215 0.00 0.00 38.78 0.00 0.00 11.14
hsa-miR-216 0.00 0.00 0.00 0.00 0.00 0.00
hsa-miR-217 0.00 0.00 9.58 0.00 0.00 0.00
hsa-miR-218 0.00 6.52 147.02 30.88 0.00 4230.55
hsa-miR-219 0.00 0.00 0.00 0.00 0.00 5.95
hsa-miR-220 0.00 0.00 0.00 0.00 0.00 0.00
hsa-miR-221 0.00 2604.05 146.04 846.12 0.00 8228.70
hsa-miR-222 15.66 12446.45 928.58 4983.01 377.84 46265.29
hsa-miR-223 14.40 165722.35 73928.30 19663.34 48.10 0.00
hsa-miR-296 361.39 9.52 222.16 957.28 241.33 709.42
Continued
285
Table C.2. continued
hsa-miR-299 0.00 0.00 0.00 0.00 0.00 0.00
hsa-miR-301 241.26 354.73 737.55 2717.90 133.28 1119.70
hsa-miR-302a 0.00 0.00 0.00 0.00 0.00 0.00
hsa-miR-302a* 0.00 0.00 0.00 0.00 0.00 0.00
hsa-miR-302b 63.42 17.29 34.34 27.70 63.59 114.87
hsa-miR-302c 32.21 0.00 0.00 0.00 0.00 0.00
hsa-miR-302c* 0.00 0.00 0.00 0.00 0.00 0.00
hsa-miR-302d 0.00 0.00 14.45 2.13 0.00 0.00
hsa-miR-320 534.90 1229.68 2515.21 1362.62 839.31 2033.67
hsa-miR-321 9062.26 6436.94 6428.65 9100.69 5068.75 9831.49
hsa-miR-323 0.00 0.00 0.00 0.00 0.00 0.00
hsa-miR-324-3p 258.23 277.85 444.47 2308.98 403.91 1186.91
hsa-miR-324-5p 41.59 109.83 146.97 950.35 44.15 883.63
hsa-miR-325 0.00 0.00 0.00 0.00 0.00 0.00
hsa-miR-326 0.00 5.28 2.91 2.79 0.00 4.13
hsa-miR-328 16.30 103.22 186.85 491.61 37.99 698.99
hsa-miR-330 31.56 14.81 21.16 55.82 6.94 38.54
hsa-miR-331 1611.60 1616.46 940.25 4585.69 1808.18 3113.31
hsa-miR-335 119.61 56.60 0.00 0.00 20.73 15.39
hsa-miR-337 0.00 0.00 0.00 0.00 0.00 0.00
hsa-miR-338 0.00 0.00 0.00 0.00 0.00 15.66
hsa-miR-339 110.36 77.48 70.42 43.05 3.57 112.03
hsa-miR-340 0.00 165.74 217.15 576.43 0.00 219.29
hsa-miR-342 1705.93 2946.96 1070.36 14531.22 690.51 1784.16
hsa-miR-345 103.55 1845.85 243.35 4617.01 31.42 714.86
hsa-miR-346 10.00 1.64 5.89 32.55 22.12 78.19
hsa-miR-361 19.35 357.66 78.53 443.88 6.25 330.99
hsa-miR-367 0.00 0.00 0.00 0.00 0.00 0.00
hsa-miR-368 0.00 0.00 0.00 0.00 0.00 0.00
hsa-miR-369 0.00 0.00 0.00 0.00 0.00 0.00
hsa-miR-370 104.46 60.29 111.53 82.01 90.94 116.97
hsa-miR-371 0.00 0.00 0.00 0.00 0.00 0.00
hsa-miR-372 122.99 100.71 118.58 110.00 93.90 232.23
hsa-miR-373 0.00 0.00 23.52 0.00 0.00 0.00
hsa-miR-374 1902.86 1720.37 1788.40 3138.22 375.59 4437.92
hsa-miR-375 0.00 0.00 25.16 24.20 0.00 467.00
hsa-miR-376a 0.00 0.00 11.92 0.00 0.00 7.46
hsa-miR-377 1.54 1.79 0.00 3.59 0.00 2.20
hsa-miR-378 117.34 213.12 325.88 74.62 28.10 241.53
hsa-miR-379 4.13 2.17 0.00 2.35 2.09 0.00
hsa-miR-380-3P 0.00 0.00 0.00 0.00 0.00 0.00
hsa-miR-380-5p 0.00 0.00 0.00 0.00 0.00 0.00
Continued
286
Table C.2. continued
hsa-miR-381 0.00 0.00 0.00 0.00 5.96 0.00
hsa-miR-382 0.00 0.00 7.66 0.00 3.48 0.00
hsa-miR-383 2.98 0.00 0.00 0.00 10.12 0.00
hsa-miR-384 0.00 0.00 0.00 0.00 0.00 0.00
hsa-miR-422b 103.70 144.22 194.45 40.90 26.31 184.74
hsa-miR-423 444.01 455.72 504.97 526.64 305.72 581.65
hsa-miR-425 224.34 564.43 265.32 504.65 10.67 179.91
let-7f(mmu) 0.00 0.00 0.00 0.00 0.00 0.00
miR-129-5p(mmu) 0.00 22.19 0.00 0.00 6.32 0.00
miR-137(mmu) 0.00 0.00 0.00 0.00 0.00 0.00
miR-224(mmu) 0.00 0.00 205.80 0.00 0.00 0.00
miR-424(mmu) 0.00 0.00 0.00 0.00 0.00 0.00
287
miRNA Adipose Bladder Brain Cervix Colon Heart Lung Liver
let-7a-1* 0.75 0.69 2.36 3.41 0.63 0.79 3.08 0.82
let-7a-2-P 59.50 77.59 382.34 782.09 94.74 133.12 63.41 12.46
let-7a-3* 7.20 3.69 30.73 24.01 4.68 2.03 22.04 2.19
let-7b* 4.40 3.75 23.77 44.83 7.94 2.61 32.00 3.67
let-7c* 0.00 0.00 10135.72 0.71 0.00 0.00 0.00 0.00
let-7d* 31.17 31.52 52.17 156.60 81.39 28.07 222.35 46.10
let-7e* 2.04 1.17 3.40 5.98 0.89 0.90 4.96 1.26
let-7f-1* 0.00 0.00 0.00 0.00 0.00 0.66 0.00 0.00
let-7f-2* 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00
let-7g* 0.00 0.00 1.73 3.05 0.00 6.58 2.86 1.34
let-7i* 0.00 0.00 3.36 22.06 1.85 14.82 2.80 0.00
MIR-001-1 13.47 27.32 0.00 69.07 15.66 593.06 2.30 0.00
MIR-001-2 110.26 70.26 104.13 291.79 100.88 495.91 20.59 0.00
MIR-007-1 20.69 25.44 54.73 37.62 16.10 21.60 50.42 4.98
MIR-007-2 0.60 1.43 4.32 0.00 0.00 2.15 0.00 0.00
MIR-007-3 8.88 8.30 3159.42 12.83 114.50 5.82 0.00 1.13
MIR-009-1-P 25.62 19.76 64.32 37.55 121.07 21.16 4.05 9.72
MIR-009-2-P 19.21 40.38 4016.2 89.78 43.60 8.29 1.40 0.00
MIR-009-3-P 0.00 0.00 26.16 0.00 0.00 3.19 0.00 0.00
MIR-010a,b 4.66 7.73 0.00 32.78 21.20 0.00 12.46 0.00
MIR-015a 13.92 13.92 12.61 60.67 5.19 24.07 43.24 0.00
MIR-015b 31.84 29.94 66.48 66.43 22.14 43.33 169.60 17.31
MIR-016-1 44.78 10.32 32.91 103.59 58.68 62.70 82.10 9.60
MIR-016-2 1.62 0.78 30.46 34.06 20.79 6.13 13.21 0.00
MIR-017-P 34.40 28.13 4.51 127.28 37.37 28.99 60.31 17.94
MIR-018 463.78 527.88 507.12 1213.8 1356.1 1089.7 598.61 468.3
MIR-019a-P 0.99 0.00 1.78 10.99 9.10 6.21 0.90 3.75
MIR-019b-1-P 2.98 0.00 0.00 2.82 14.65 0.00 2.25 0.00
MIR-019b-2-P 0.00 0.00 1.93 0.00 0.00 0.00 0.00 0.00
MIR-020 0.00 1.49 0.00 4.66 0.00 0.00 3.07 0.00
MIR-021 726.86 4845.49 193.11 4116.8 6496.0 5296.2 2805.1 570.9
MIR-022-P 28.68 1.61 49.54 208.95 77.59 64.91 36.34 15.39
MIR-023a 95.83 16.55 29.70 1041.6 187.68 88.61 90.32 20.74
MIR-023b-P 98.35 54.73 167.75 804.29 108.67 150.83 133.93 38.30
MIR-024-1,2 1164.61 1651.96 2069.82 10869.64 3552.47 3825.21 1994.89 1121.87
Continued
Table C.3. microRNA precursor expression in 22 different normal human tissues
and cells. All data was generated using real-time PCR and SYBR green detection
except (*) which was assayed using TaqMan probes. Data is presented as copies of
microRNA per cell. (P) Real-time PCR was performed on the microRNA primary
precursor.
288
Table C.3. continued
MIR-025 13.12 6.58 56.07 59.11 63.11 45.35 32.25 27.79
MIR-026a-1,2 0.00 10.06 58.38 52.45 4.14 16.27 15.28 0.00
MIR-026b 5.44 1.98 4.70 7.50 2.85 11.80 2.84 2.09
MIR-027a, b 6.17 37.91 8.20 407.18 30.76 40.89 60.72 10.59
MIR-028 8.95 7.21 0.00 30.84 8.61 6.65 5.48 0.00
MIR-029a,c 30.74 11.75 14.38 95.23 12.30 17.92 17.48 0.00
MIR-029b-1,2 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00
MIR-030a,e 46.08 18.20 34.92 53.74 1.86 52.90 90.60 5.34
MIR-030b 90.44 28.99 72.64 162.46 81.66 215.04 77.58 53.26
MIR-030c-1,2 20.46 23.57 8.63 118.63 62.55 104.76 44.04 0.00
MIR-030d 93.16 13.61 15.84 47.13 23.93 63.52 73.58 0.00
MIR-031-P 16.66 18.93 61.40 63.24 24.67 29.96 5.29 1.82
MIR-032 49.61 12.65 36.33 29.74 34.87 13.77 34.25 5.14
MIR-033 3.72 0.00 0.00 3.30 4.68 0.00 3.49 0.00
MIR-034a 32.17 32.73 16.21 76.58 23.81 31.50 30.02 1.65
MIR-034b 1.66 0.00 23.86 5.67 4.13 9.75 1.96 0.00
MIR-092-1 0.00 0.00 7.31 33.61 0.00 10.08 5.28 2.05
MIR-092-2-P 0.00 0.00 7.84 0.00 3.28 0.00 0.00 0.00
MIR-093 9.89 27.13 22.54 25.63 47.26 26.85 9.15 16.41
MIR-095 11.46 0.00 41.77 10.74 6.99 10.67 0.78 0.00
MIR-096-P 27.63 14.29 84.64 26.31 44.66 65.84 4.95 14.22
MIR-098 1898.10 1058.63 1895.2 3204.8 1773.8 2686.1 833.86 543.0
MIR-099a 0.00 2.67 1.66 2.93 8.56 3.13 4.05 0.00
MIR-099b 181.14 140.46 206.02 572.16 252.30 363.67 181.13 22.74
MIR-100 69.39 454.57 959.06 1267.8 227.85 389.88 264.97 51.60
MIR-101-1 1.64 0.00 1.97 4.08 10.76 0.00 1.63 0.00
MIR-101-2 14.65 13.80 30.85 76.89 18.87 19.25 17.05 64.83
MIR-103-1,2 348.90 182.41 1059.69 1036.13 966.82 1007.07 437.91 245.06
MIR-105-1,2 1.06 1.64 1.00 0.87 5.34 12.00 1.79 0.00
MIR-106a-P 0.00 0.00 0.00 0.00 0.00 4.16 0.00 0.00
MIR-106b 82.19 251.39 199.94 382.05 265.91 233.14 150.92 57.76
MIR-107 576.18 678.21 1464.2 1629.8 1841.9 2065.3 713.31 517.7
MIR-108 38.28 33.63 41.75 64.64 19.32 21.85 11.82 10.00
MIR-122a 0.00 0.00 2.96 0.00 0.00 0.00 0.00 0.67
MIR-124a-1,2,3 0.00 6.46 10029.82 18.13 6.02 3.72 4.38 9.11
MIR-125a 55.77 154.82 176.16 286.06 68.36 152.02 70.14 9.10
MIR-125b-1 42.62 131.17 348.57 556.36 58.62 128.66 30.18 1.57
MIR-125b-2 0.00 12.74 34.74 57.96 1.72 12.35 7.60 11.94
MIR-126 1009.52 675.68 265.20 915.89 290.71 903.77 898.90 192.6
MIR-127 77.52 64.04 118.27 144.14 118.64 130.83 28.18 51.12
MIR-128a 45.31 21.71 790.52 183.16 80.10 77.42 24.54 1.29
Continued
289
Table C.3. continued
MIR-128b 1.02 1.04 31.98 3.08 0.93 0.97 1.50 4.94
MIR-129-2 1.30 14.76 46.20 8.57 4.96 2.81 0.83 3.43
MIR-130a-P 13.43 4.44 47.72 30.76 24.73 5.87 4.44 0.00
MIR-130b 3.18 13.19 15.82 0.59 0.85 13.76 0.00 0.00
MIR-132 10.97 5.28 26.13 40.88 10.28 12.09 16.46 17.87
MIR-133a-1,2 12.25 19.25 55.32 34.69 24.94 158.24 0.00 0.00
MIR-133b 0.65 0.70 1.99 1.39 0.87 3.15 0.00 4.90
MIR-134 4.62 2.18 74.35 53.30 5.84 2.74 6.89 0.00
MIR-135a-1,2 39.70 15.12 64.95 47.51 65.65 1.11 13.94 0.00
MIR-135b-P 16.11 0.00 3.41 14.18 9.95 0.00 6.41 0.00
MIR-136 0.00 8.89 28.78 125.03 11.29 0.00 6.21 1.88
MIR-137* 0.00 0.00 16.33 1.48 0.00 0.00 0.00 0.00
MIR-138-1,2 0.00 0.00 18.71 0.00 0.00 0.00 0.00 0.00
MIR-139 33.48 25.96 9.37 43.23 43.28 40.62 18.43 10.91
MIR-140 1.87 0.00 1.21 1.38 1.84 0.00 1.11 0.00
MIR-141 7.97 3.16 0.00 4.18 15.83 0.00 12.38 2.15
MIR-142-P 33.77 7.39 3.82 30.93 41.94 3.41 22.62 0.00
MIR-143 104.91 277.38 8.82 628.55 161.54 62.42 42.98 2.63
MIR-144 10.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00
MIR-145-P 525.56 268.21 53.67 3619.5 489.22 178.98 29.97 8.57
MIR-146 0.00 0.00 0.00 0.00 2.41 0.00 0.00 0.00
MIR-147 13.18 13.15 45.86 0.00 11.89 12.60 21.00 11.62
MIR-148a-P 2.01 0.00 0.00 0.00 0.00 1.09 0.86 0.00
MIR-148b-P 17.41 0.00 22.42 53.65 16.64 14.64 6.68 5.47
MIR-149 16.82 23.54 335.85 45.16 7.03 43.43 9.67 0.87
MIR-150 4.22 4.25 4.55 4.30 7.61 4.20 12.49 0.00
MIR-151 7.01 1.01 3.96 3.03 6.84 5.01 2.87 1.24
MIR-152 51.53 31.46 46.43 109.07 17.90 26.29 14.48 18.13
MIR-153-1,2 11.74 1.43 43.73 2.30 5.97 2.73 4.57 0.00
MIR-154 1.06 0.00 36.34 28.47 13.59 12.81 7.73 12.24
MIR-155 184.38 32.00 31.77 54.83 32.05 76.53 72.47 16.20
MIR-181a,c 23.73 33.72 138.14 80.11 74.92 317.05 29.04 9.16
MIR-181b-1,2 0.87 0.00 2.67 2.08 0.00 0.00 0.00 0.00
MIR-182 12.15 16.80 22.60 28.79 11.59 24.78 11.73 8.38
MIR-183 2.41 0.00 0.00 3.81 3.27 4.47 3.85 0.00
MIR-184 7.19 1.57 97.01 7.60 8.61 7.59 1.67 0.00
MIR-185 52.08 36.10 38.94 40.53 35.03 35.28 7.29 20.37
MIR-186-P 124.97 104.79 346.03 373.86 177.41 107.09 20.83 42.75
MIR-187 0.00 0.00 0.00 1.26 0.00 0.00 1.81 0.00
MIR-188b-P 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00
MIR-190 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00
Continued
290
Table C.3. continued
MIR-191 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00
MIR-192 1.32 0.00 0.00 0.00 0.00 0.00 0.00 0.00
MIR-193 31.16 21.52 0.00 23.81 10.55 48.89 5.36 11.17
MIR-194-1,2 0.00 0.00 3.62 0.00 0.00 0.00 0.91 0.00
MIR-195-P 26.84 0.88 32.63 34.48 13.20 12.65 3.63 0.00
MIR-196a-1 0.00 0.00 0.00 0.00 40.11 0.00 0.00 0.00
MIR-196a-2 5.99 1.42 2.61 13.71 5.15 0.00 0.00 0.00
MIR-196b 1575.64 3263.01 0.00 13804.43 2520.04 6.07 29.39 0.00
MIR-197 22.00 9.81 67.67 95.68 83.04 37.20 12.42 16.33
MIR-198 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00
MIR-199a-1-P 3.47 1.92 0.84 1.55 8.00 5.37 2.20 2.01
MIR-199a-2-P 12.45 5.14 71.30 124.54 12.83 20.45 6.26 14.25
MIR-199b-P 21.25 40.38 28.09 104.56 23.94 26.69 6.34 0.00
MIR-200a-P 153.44 152.68 589.43 268.50 405.50 200.99 57.85 60.06
MIR-200b 0.00 9.55 1.77 31.39 72.70 0.00 6.09 0.00
MIR-200c 0.00 1.37 1.18 0.00 9.95 0.00 0.00 0.00
MIR-203 0.00 0.00 0.00 17.14 0.00 0.00 0.00 0.00
MIR-204 8.32 6.78 10.97 3.68 16.56 7.39 0.00 0.00
MIR-205 33.37 23.61 0.00 65.63 0.00 10.34 6.31 0.00
MIR-206 16.69 16.52 0.00 17.98 0.00 0.00 0.00 0.00
MIR-208 5.87 2.44 21.67 0.00 0.00 2256.4 0.00 1.73
MIR-210 112.55 174.54 41.88 80.49 9.98 122.63 73.45 20.63
MIR-211 52.17 10.25 23.82 54.99 28.07 31.18 12.56 12.73
MIR-212 186.76 202.37 135.24 717.92 202.71 152.57 80.99 28.90
MIR-213 2.18 0.00 30.36 13.32 12.71 17.02 16.14 0.00
MIR-214 9.77 14.66 39.65 9.35 8.80 5.03 4.74 12.50
MIR-215 18.57 0.00 14.16 40.69 28.00 24.88 8.34 6.06
MIR-216 0.00 0.00 5.23 1.04 1.04 0.00 0.00 0.00
MIR-217 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00
MIR-218-1,2 94.62 238.06 113.61 991.28 70.39 103.03 166.77 2.08
MIR-219-1,2 0.00 9.60 3.57 0.94 14.92 0.00 3.71 0.00
MIR-220 1.58 8.57 519.70 3.87 1.78 2.25 0.00 0.00
MIR-221 35.50 53.29 27.42 241.34 53.48 25.43 45.50 0.00
MIR-222-P 13.61 13.26 5.65 130.65 23.59 0.00 18.78 18.17
MIR-223 3.16 0.00 5.09 4.41 1.96 3.21 5.32 0.00
MIR-224 18.79 20.36 11.86 117.02 37.94 36.23 10.37 0.00
MIR-296 0.00 2.44 11.84 12.02 0.00 7.84 0.00 0.00
MIR-299-P 18.74 9.35 7.73 10.49 9.31 0.00 4.43 13.83
MIR-301 0.00 0.00 16.34 11.01 23.07 16.81 13.88 18.67
MIR-302 6.85 6.46 19.22 0.00 19.09 0.00 3.55 0.00
MIR-302b 20.25 8.75 13.74 15.59 15.99 13.30 5.78 0.00
Continued
291
Table C.3. continued
MIR-302c 0.00 0.00 0.69 2.33 1.69 0.00 0.59 0.00
MIR-302d 1.30 0.00 0.00 0.00 1.05 0.00 0.00 0.00
MIR-320 4.22 8.04 3.34 11.35 7.75 9.22 10.76 3.61
MIR-321 2026.23 8509.22 10664.52 6486.85 2916.11 11548.94 862.79 3178.97
MIR-323 8.53 5.45 95.13 38.68 10.59 12.65 14.09 2.19
MIR-324-P 43.01 45.07 37.01 61.30 15.46 77.17 22.75 6.68
MIR-325 444.34 5.85 202.97 8.87 2.24 9.07 3.11 3.49
MIR-326-P 4.44 2.57 22.99 6.92 5.23 1.47 7.59 2.31
MIR-328 0.92 2.71 7.70 2.68 2.01 1.69 0.00 1.59
MIR-330 0.00 0.00 55.77 1.29 0.00 3.59 5.94 0.00
MIR-331-P 27.83 53.55 30.79 132.00 18.73 21.44 42.89 6.38
MIR-335 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00
MIR-337 0.00 0.00 3.39 15.66 10.99 0.00 0.00 0.00
MIR-338 0.00 0.00 50.94 0.00 3.03 6.46 7.40 12.68
MIR-339 347.06 414.19 800.47 597.76 329.61 584.73 181.97 123.1
MIR-340 284.94 307.41 1266.6 438.59 344.28 300.20 158.72 40.21
MIR-342 12.96 14.62 3.99 6.29 43.73 14.89 11.60 19.13
MIR-345 7.98 17.59 23.62 24.49 13.28 17.78 6.65 1.83
MIR-346 81.49 72.61 108.90 84.95 99.27 102.51 37.66 76.24
MIR-361 3.70 1.97 11.80 7.27 2.45 1.40 11.50 2.31
MIR-367 1.74 1.12 1.00 4.80 2.01 0.00 0.86 0.00
MIR-368 1.69 0.00 28.96 20.45 6.05 6.10 0.00 2.21
MIR-369 0.00 0.00 12.16 9.51 0.00 0.00 0.00 0.00
MIR-370 36.15 58.57 491.75 506.66 64.94 23.06 46.88 6.25
MIR-371 0.00 6.08 11.60 0.00 1.81 1.51 0.00 0.00
MIR-372 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00
MIR-373 0.00 0.00 0.00 0.00 0.00 0.00 0.86 0.00
MIR-374-P 0.77 0.00 1.07 0.00 0.00 0.00 0.00 0.00
MIR-375 3.00 0.00 2.22 9.49 37.45 0.00 10.75 0.00
MIR-376 0.00 3.08 140.06 109.45 23.68 10.45 0.00 0.00
MIR-377 0.00 0.00 11.68 18.39 7.22 0.00 0.00 0.00
MIR-378 0.90 0.00 0.00 0.00 0.00 0.00 0.00 0.00
MIR-379 31.52 40.21 234.52 291.68 52.29 55.40 23.38 6.76
MIR-380 23.30 0.00 183.03 88.59 39.45 22.94 7.63 0.00
MIR-381 0.00 0.89 0.00 0.00 0.00 0.00 0.00 0.00
MIR-382 5.51 8.48 13.29 32.67 4.18 3.65 8.76 0.00
MIR-383 1.40 0.00 14.22 2.56 1.60 0.00 0.00 0.00
MIR-384 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00
MIR-422b 0.63 0.86 2.74 0.00 0.80 0.00 0.00 0.00
MIR-423 10.68 10.89 18.99 16.24 10.71 12.92 5.44 0.00
MIR-424 0.00 10.88 0.00 99.02 2.91 0.00 0.00 0.00
Continued
292
Table C.3. continued
MIR-425 18.94 0.00 19.24 13.63 2.66 24.37 9.42 0.00
miRNA Kidney Esophagus Skeletal Muscle
Ovary Pancreas Placenta Prostate
let-7a-1* 2.67 0.67 0.00 2.80 0.00 4.62 0.99
let-7a-2-P 80.30 27.18 6.55 540.35 5.83 1314.62 62.17
let-7a-3* 8.55 3.97 1.68 46.81 2.17 24.25 14.78
let-7b* 16.56 5.99 6.16 88.87 5.85 20.91 8.05
let-7c* 0.00 0.00 0.00 0.77 0.00 0.00 0.00
let-7d* 175.14 30.88 97.64 208.96 20.97 83.62 24.85
let-7e* 4.38 1.85 0.00 8.44 0.75 5.22 1.63
let-7f-1* 0.00 0.00 0.00 3.54 0.88 6.73 2.68
let-7f-2* 0.00 0.00 0.00 0.00 0.00 0.00 0.00
let-7g* 1.64 0.00 2.31 24.85 6.30 90.73 9.94
let-7i* 2.00 1.64 11.79 58.97 16.98 43.95 18.59
MIR-001-1 0.00 45.93 789.78 21.24 0.00 0.00 45.50
MIR-001-2 26.67 97.04 963.41 121.68 26.64 90.52 144.03
MIR-007-1 20.21 15.14 2.83 37.16 9.66 27.09 15.61
MIR-007-2 0.00 0.00 0.00 0.00 0.71 0.00 0.00
MIR-007-3 6.23 8.57 0.00 28.99 931.86 0.00 0.00
MIR-009-1-P 29.37 13.90 13.74 24.83 7.53 17.52 38.68
MIR-009-2-P 63.01 33.59 4.70 111.30 34.36 45.60 45.27
MIR-009-3-P 0.00 0.00 0.00 0.00 0.00 0.00 0.00
MIR-010a,b 111.32 9.65 0.00 56.68 12.95 22.86 14.37
MIR-015a 15.82 6.11 10.72 38.63 0.00 135.08 5.37
MIR-015b 38.97 97.67 30.79 59.17 11.55 171.58 68.60
MIR-016-1 85.94 37.83 59.40 122.51 44.15 207.44 576.76
MIR-016-2 1.54 5.30 1.94 11.34 2.58 1.01 1.90
MIR-017-P 36.86 23.12 12.74 156.99 16.25 373.29 112.46
MIR-018 1424.95 542.80 455.34 1771.70 807.40 1309.32 363.43
MIR-019a-P 7.23 0.98 0.00 12.87 0.85 11.20 0.00
MIR-019b-1-P 7.59 0.00 0.00 2.08 0.00 23.18 5.09
MIR-019b-2-P 3.96 0.00 0.00 0.00 0.00 14.00 0.00
MIR-020 0.00 0.00 0.00 1.96 0.00 4.28 1.73
MIR-021 6882.19 5295.46 939.05 1427.79 2624.10 1657.19 1548.93
MIR-022-P 39.80 9.22 21.76 366.13 0.00 180.77 2.75
MIR-023a 48.60 44.38 26.09 382.38 10.42 576.04 52.50
MIR-023b-P 119.19 55.78 68.90 887.18 33.19 389.65 80.58
MIR-024-1,2 3505.26 4960.45 3245.22 7158.98 2515.70 8900.50 3574.82
MIR-025 38.56 12.37 55.15 98.13 8.26 120.23 48.30
MIR-026a-1,2 17.93 1.42 0.00 13.17 22.71 18.64 19.16
Continued
293
Table C.3. continued
MIR-026b 10.16 6.82 4.53 3.36 4.16 14.54 4.06
MIR-027a, b 28.23 38.85 32.60 159.45 0.00 302.83 43.74
MIR-028 1.62 5.92 7.65 6.24 0.00 9.87 0.00
MIR-029a,c 28.40 3.33 2.36 71.31 5.48 75.32 8.71
MIR-029b-1,2 0.00 0.00 0.00 0.00 0.00 0.00 0.00
MIR-030a,e 117.16 8.78 8.84 49.69 7.42 499.41 36.98
MIR-030b 259.04 29.58 56.53 190.04 56.20 1130.36 14.15
MIR-030c-1,2 75.33 24.75 21.56 226.18 16.41 74.84 25.15
MIR-030d 79.50 7.22 0.00 77.36 0.00 843.44 25.51
MIR-031-P 15.21 14.42 4.15 17.36 6.93 24.02 17.28
MIR-032 41.50 16.68 38.30 108.15 21.98 40.95 18.73
MIR-033 0.00 0.00 0.00 0.00 0.00 3.48 0.00
MIR-034a 23.68 14.43 18.64 195.36 25.33 40.06 55.96
MIR-034b 0.00 1.57 0.00 0.00 0.00 35.42 0.00
MIR-092-1 13.07 0.00 0.00 7.63 5.88 25.61 0.00
MIR-092-2-P 17.74 0.00 0.00 0.00 0.00 13.39 0.00
MIR-093 40.09 10.70 0.00 34.32 14.57 28.86 13.17
MIR-095 2.95 10.85 14.85 2.88 0.00 0.00 0.00
MIR-096-P 49.49 37.35 50.85 26.64 19.61 16.88 14.93
MIR-098 3333.33 742.35 1343.40 5109.21 741.84 849.31 731.19
MIR-099a 3.03 1.20 0.00 7.02 1.99 4.86 1.59
MIR-099b 421.87 147.96 77.07 715.83 156.68 751.92 177.90
MIR-100 844.01 350.45 93.85 2389.21 70.90 2932.21 451.08
MIR-101-1 1.41 0.00 0.00 0.00 0.00 0.00 0.00
MIR-101-2 43.12 24.34 57.77 60.48 55.53 14.65 16.67
MIR-103-1,2 1304.78 746.74 790.22 1374.23 254.78 858.16 623.04
MIR-105-1,2 1.52 2.68 1.21 10.67 0.00 1.32 6.03
MIR-106a-P 1.11 0.00 0.00 0.00 0.00 1.05 0.00
MIR-106b 291.23 218.75 203.83 625.82 103.98 266.55 187.79
MIR-107 2183.38 1228.07 1078.47 2007.52 376.09 1462.49 763.88
MIR-108 11.87 11.98 7.60 21.02 6.99 47.12 28.87
MIR-122a 0.00 0.00 0.00 0.00 0.00 0.00 0.00
MIR-124a-1,2,3 71.88 8.38 5.50 11.31 0.00 0.00 35.51
MIR-125a 352.13 79.37 34.63 467.62 48.17 469.15 141.71
MIR-125b-1 102.23 47.98 26.20 702.85 29.93 148.68 67.67
MIR-125b-2 16.23 12.02 0.00 60.55 0.00 0.00 4.15
MIR-126 758.40 466.62 249.11 573.94 171.26 4231.07 429.80
MIR-127 78.63 111.59 49.85 52.50 46.12 157.32 46.62
MIR-128a 98.57 29.06 19.12 87.45 45.76 67.14 33.08
MIR-128b 0.00 16.50 1.85 5.13 0.00 12.65 1.11
MIR-129-2 10.39 8.45 0.00 4.80 7.84 5.67 2.64
Continued
294
Table C.3. continued
MIR-130a-P 1.80 6.01 202.06 37.35 14.08 22.04 9.98
MIR-130b 1.86 1.10 1279.11 0.00 14.15 0.72 0.79
MIR-132 4.01 16.34 0.00 18.33 11.16 8.49 21.54
MIR-133a-1,2 17.13 11.08 0.00 13.69 19.75 34.01 20.07
MIR-133b 12.90 7.00 44.97 0.00 0.00 0.00 22.65
MIR-134 3.34 3.76 20.43 66.74 19.88 116.51 4.08
MIR-135a-1,2 17.58 10.94 14.56 630.15 34.48 21.64 26.10
MIR-135b-P 40.19 0.00 397.07 6.65 0.00 39.79 0.00
MIR-136 13.82 14.83 36.01 44.08 23.67 371.97 4.95
MIR-137* 0.00 0.00 0.00 0.00 0.00 1.55 0.00
MIR-138-1,2 0.00 0.00 0.00 0.00 0.00 0.00 0.00
MIR-139 33.40 56.28 35.44 14.73 33.24 28.84 22.33
MIR-140 1.53 0.00 0.00 0.00 0.00 0.00 0.00
MIR-141 13.21 13.94 1.52 0.00 28.03 20.57 4.51
MIR-142-P 1.36 1.27 13.71 22.22 10.31 50.83 0.00
MIR-143 55.43 349.42 5.30 190.18 6.14 65.88 121.08
MIR-144 0.00 0.00 0.00 0.00 0.00 0.00 0.00
MIR-145-P 85.34 256.59 5.41 1441.42 11.90 294.34 99.01
MIR-146 0.00 1.45 0.00 1.67 0.00 1.02 0.00
MIR-147 12.85 1.11 9.70 7.87 0.00 18.02 12.14
MIR-148a-P 0.00 0.00 0.00 0.00 0.00 0.00 0.00
MIR-148b-P 25.24 2.86 7.37 65.17 4.88 53.62 19.77
MIR-149 13.39 54.49 22.18 40.42 15.19 165.32 113.17
MIR-150 3.63 11.30 3.26 13.69 2.11 7.11 2.03
MIR-151 9.79 11.32 1.83 0.00 1.18 2.54 17.07
MIR-152 80.91 41.82 48.18 83.55 31.52 40.20 74.14
MIR-153-1,2 0.00 0.00 0.00 3.70 2.30 9.09 12.08
MIR-154 13.20 13.09 16.93 28.52 37.10 76.59 13.13
MIR-155 63.11 55.89 19.17 104.65 22.00 14.08 56.92
MIR-181a,c 93.44 18.78 20.71 87.43 31.46 64.89 60.17
MIR-181b-1,2 0.00 0.00 0.00 0.00 0.00 1.61 0.00
MIR-182 139.76 96.09 548.20 11.64 123.54 22.62 71.04
MIR-183 5.37 3.40 1.71 0.00 4.84 0.00 6.09
MIR-184 4.88 5.39 0.91 3.35 15.65 2.59 3.38
MIR-185 42.62 42.39 19.25 25.51 47.78 32.75 18.16
MIR-186-P 175.36 69.91 10.38 431.17 82.93 46.83 198.59
MIR-187 0.00 0.00 0.00 6.13 0.00 0.00 0.00
MIR-188b-P 0.00 0.00 0.00 0.00 0.00 0.00 0.00
MIR-190 0.00 0.00 0.00 0.00 0.00 0.00 0.00
MIR-191 0.78 0.00 0.00 1.17 0.00 0.00 0.00
MIR-192 0.00 0.00 0.00 0.00 0.00 0.00 0.00
Continued
295
Table C.3. continued
MIR-193 10.29 18.59 36.16 38.48 3.20 10.64 17.26
MIR-194-1,2 0.00 0.68 0.00 0.00 0.00 0.00 0.00
MIR-195-P 5.76 17.92 13.81 10.82 3.39 32.50 9.47
MIR-196a-1 0.92 1.66 0.00 0.00 0.00 0.00 1.25
MIR-196a-2 42.51 0.00 15.89 1.80 0.00 11.23 6.36
MIR-196b 5283.01 27.73 4189.00 2575.39 0.00 569.86 2739.71
MIR-197 73.06 108.13 25.63 99.49 31.03 57.37 24.73
MIR-198 0.00 0.00 0.00 0.00 0.00 0.00 0.00
MIR-199a-1-P 4.80 5.25 4.28 5.74 2.72 5.06 1.40
MIR-199a-2-P 25.76 1.59 4.81 348.71 0.00 57.92 3.87
MIR-199b-P 9.21 19.12 18.63 50.74 19.22 22.97 30.51
MIR-200a-P 142.29 167.16 145.97 222.65 74.56 178.21 148.86
MIR-200b 28.17 9.79 2.65 22.50 17.78 0.00 17.43
MIR-200c 0.00 0.00 0.00 0.00 0.00 0.00 5.02
MIR-203 0.78 90.01 0.00 0.00 0.00 0.00 0.00
MIR-204 65.37 9.14 0.00 8.30 5.15 0.00 0.00
MIR-205 7.10 147.52 0.00 0.00 0.00 28.07 107.83
MIR-206 14.04 113.46 2116.83 0.00 0.00 0.00 29.30
MIR-208 2.52 0.00 175.88 0.00 0.00 9.72 11.94
MIR-210 155.54 352.68 28.66 77.17 45.46 247.18 57.23
MIR-211 12.63 35.83 14.33 10.22 2.61 70.58 16.09
MIR-212 95.24 143.92 106.87 255.19 12.72 58.75 99.06
MIR-213 32.79 15.88 0.00 3.04 5.32 40.04 4.67
MIR-214 2.67 4.41 13.66 12.91 3.41 17.47 6.37
MIR-215 12.17 6.69 4.82 39.82 13.37 26.23 9.89
MIR-216 0.00 1.22 0.00 1.90 15.32 2.48 2.35
MIR-217 0.00 0.00 0.00 0.00 0.00 0.00 0.00
MIR-218-1,2 190.38 159.98 19.42 276.72 32.74 109.51 175.15
MIR-219-1,2 11.72 4.64 1.99 4.41 0.00 7.95 2.78
MIR-220 1.76 5.06 2.20 44.12 0.00 0.00 2.08
MIR-221 22.57 77.05 60.47 230.47 0.70 95.65 58.08
MIR-222-P 10.99 12.91 0.00 62.77 0.00 79.72 17.96
MIR-223 0.00 0.00 0.00 1.15 0.00 4.59 0.00
MIR-224 19.57 32.26 15.73 4.90 17.18 231.43 34.64
MIR-296 0.00 0.00 0.00 0.00 0.00 0.00 0.00
MIR-299-P 9.84 8.23 3.83 1.80 18.76 10.37 8.52
MIR-301 31.24 10.83 0.00 47.99 11.44 32.99 11.66
MIR-302 15.31 4.77 0.00 4.21 0.00 11.11 13.53
MIR-302b 25.22 7.94 0.00 27.78 0.00 4.70 16.07
MIR-302c 1.28 0.00 0.00 0.98 0.89 1.31 0.00
MIR-302d 0.00 1.28 0.00 0.00 0.00 0.00 0.00
Continued
296
Table C.3. continued
MIR-320 6.42 9.47 3.11 21.59 6.26 23.10 8.75
MIR-321 4550.20 6835.94 1854.20 2747.62 1464.55 4763.74 24202.07
MIR-323 9.88 3.73 11.18 15.81 35.17 104.00 5.94
MIR-324-P 21.74 17.81 29.72 32.27 18.16 74.18 21.36
MIR-325 0.00 3.05 3.17 5.30 0.00 1.12 0.00
MIR-326-P 2.07 0.89 1.31 5.68 2.17 17.71 5.04
MIR-328 2.49 8.93 0.00 6.26 42.26 2.48 2.02
MIR-330 15.20 2.73 9.94 4.64 6.24 12.76 4.78
MIR-331-P 65.64 36.27 66.82 123.87 0.00 92.79 52.04
MIR-335 0.00 0.00 0.00 0.00 0.00 0.00 0.00
MIR-337 0.00 0.00 0.00 0.00 1.52 31.84 0.00
MIR-338 0.00 0.00 2.47 0.92 0.00 0.00 4.40
MIR-339 284.65 361.44 353.05 616.01 352.49 266.35 452.05
MIR-340 301.38 225.23 199.98 633.11 154.87 145.67 279.34
MIR-342 12.51 13.47 0.00 35.49 17.36 18.67 6.35
MIR-345 11.87 12.13 8.49 18.93 15.16 15.08 7.10
MIR-346 64.53 100.82 96.58 100.12 76.30 69.58 87.84
MIR-361 8.42 3.92 7.40 33.86 2.65 16.99 2.33
MIR-367 0.00 0.92 0.00 1.00 0.91 0.00 0.71
MIR-368 2.39 1.37 0.00 4.35 9.77 54.22 0.00
MIR-369 0.00 0.00 0.00 6.69 8.82 24.90 0.00
MIR-370 17.47 41.69 44.11 277.54 123.46 813.43 129.97
MIR-371 5.60 4.37 0.00 1.99 1.67 0.00 0.00
MIR-372 0.00 0.00 0.00 0.00 0.00 0.00 0.00
MIR-373 0.00 0.00 0.00 0.00 0.00 0.00 1.56
MIR-374-P 0.00 0.00 0.00 0.00 0.00 0.00 1.88
MIR-375 0.00 19.72 1.08 0.00 42.33 0.00 2.68
MIR-376 4.45 3.72 0.00 32.03 26.12 295.93 5.65
MIR-377 10.69 4.28 0.00 13.93 10.84 117.97 16.92
MIR-378 0.00 0.00 1.17 0.00 0.00 0.00 0.00
MIR-379 35.74 24.57 22.77 196.88 164.84 406.09 27.64
MIR-380 9.10 11.44 0.00 76.74 28.55 192.46 12.91
MIR-381 0.00 0.00 0.00 0.00 0.00 0.00 0.00
MIR-382 2.94 0.00 0.00 4.41 0.00 41.93 0.00
MIR-383 0.00 1.77 0.00 2.52 0.00 0.00 2.90
MIR-384 0.00 0.00 0.00 0.00 0.00 0.00 0.00
MIR-422b 0.00 1.38 0.00 0.00 1.48 0.00 0.00
MIR-423 27.54 10.66 3.43 6.96 1.83 13.64 7.97
MIR-424 15.37 0.00 30.63 55.54 0.00 44.63 0.00
MIR-425 9.72 15.61 7.35 14.28 8.43 13.93 0.00
Continued
297
Table C.3. continued
miRNA Spleen Small
Intestine Trachea Thymus Thyroid Testes
Normal B cell
let-7a-1* 3.76 2.02 4.22 1.03 1.54 1.98 5.58
let-7a-2-P 26.98 15.13 143.89 122.84 68.63 103.55 13.63
let-7a-3* 15.99 8.80 42.01 6.10 5.28 9.11 9.56
let-7b* 8.56 5.37 30.85 6.55 6.52 24.05 9.11
let-7c* 0.00 0.00 0.00 0.00 0.00 0.00 0.00
let-7d* 41.06 15.57 70.48 47.31 74.16 98.78 118.02
let-7e* 0.66 0.00 0.75 0.92 1.10 2.40 0.00
let-7f-1* 4.18 0.00 0.00 0.00 0.00 0.89 10.06
let-7f-2* 0.00 0.00 0.00 0.00 0.00 0.00 1.31
let-7g* 24.78 1.36 0.00 4.44 1.62 7.14 106.34
let-7i* 51.62 1.67 4.43 11.52 19.86 16.06 118.28
MIR-001-1 0.00 4.67 14.73 0.00 22.62 24.42 0.00
MIR-001-2 121.34 87.09 211.46 163.23 105.04 140.26 124.30
MIR-007-1 62.90 44.46 57.11 42.30 33.41 31.67 879.06
MIR-007-2 0.00 0.00 1.25 1.20 0.72 0.00 0.96
MIR-007-3 4.90 116.63 2.41 13.43 0.00 1071.3 10.99
MIR-009-1-P 32.98 17.31 38.14 12.39 20.91 33.38 68.53
MIR-009-2-P 32.40 61.84 46.46 44.75 11.89 57.90 68.33
MIR-009-3-P 0.00 0.00 0.00 0.00 0.00 0.00 0.00
MIR-010a,b 29.48 48.88 12.89 12.43 7.35 44.76 7.17
MIR-015a 187.92 28.53 82.41 17.29 11.00 19.21 576.46
MIR-015b 269.72 47.19 109.93 331.21 43.99 156.56 450.15
MIR-016-1 494.76 107.08 184.33 578.36 34.30 179.48 745.52
MIR-016-2 7.13 48.31 20.17 79.11 4.39 24.40 27.50
MIR-017-P 371.57 33.60 176.83 43.16 33.52 42.23 975.57
MIR-018 2315.1 1424.52 972.14 2457.57 408.89 1534.5 4032.20
MIR-019a-P 24.24 4.00 5.49 1.61 0.93 2.81 80.23
MIR-019b-1-P 19.08 0.00 3.84 0.00 4.52 10.31 77.38
MIR-019b-2-P 0.00 0.00 0.00 0.00 0.00 0.74 5.05
MIR-020 0.00 0.00 4.01 0.00 4.56 0.00 24.15
MIR-021 4264.4 5613.86 3144.56 1882.57 764.66 2173.4 709.24
MIR-022-P 174.22 95.63 267.69 18.31 5.42 24.09 736.45
MIR-023a 132.66 112.12 258.68 40.60 50.93 46.03 352.13
MIR-023b-P 189.68 72.98 317.74 20.83 36.97 47.51 51.81
MIR-024-1,2 2610.1 3192.14 4313.32 1471.94 1017.73 1579.6 894.92
MIR-025 114.28 58.79 64.42 91.20 35.45 74.46 170.41
MIR-026a-1,2 20.65 28.55 36.90 19.21 10.46 33.74 2.61
MIR-026b 15.21 18.21 5.94 5.12 15.10 2.35 12.25
MIR-027a, b 109.63 21.79 125.60 17.08 11.24 41.09 314.92
Continued
298
Table C.3. continued
MIR-028 1.29 9.70 7.20 3.25 0.00 1.28 29.30
MIR-029a,c 77.39 16.40 56.27 32.23 8.54 44.52 178.18
MIR-029b-1,2 0.00 0.00 0.00 0.00 0.00 0.00 0.00
MIR-030a,e 51.39 10.42 52.14 27.41 28.25 22.19 44.61
MIR-030b 230.63 153.23 204.49 94.47 35.04 155.84 35.93
MIR-030c-1,2 295.80 92.35 76.67 54.82 0.00 40.00 220.32
MIR-030d 114.20 16.05 123.63 0.00 16.23 19.93 66.64
MIR-031-P 22.82 38.43 83.25 16.58 6.32 34.47 56.06
MIR-032 108.94 83.47 48.01 31.77 51.41 141.25 69.72
MIR-033 0.00 0.00 4.40 0.00 0.00 0.00 70.27
MIR-034a 26.79 24.42 73.96 48.13 21.61 128.14 6.50
MIR-034b 0.00 0.00 13.40 1.54 0.00 20.13 0.00
MIR-092-1 12.85 0.00 3.09 0.00 6.49 5.50 154.46
MIR-092-2-P 35.84 0.00 6.09 0.00 0.00 0.00 0.00
MIR-093 38.33 19.47 26.04 108.38 13.60 53.36 77.21
MIR-095 13.11 0.00 0.00 0.00 6.33 0.00 15.95
MIR-096-P 27.08 58.54 52.25 18.93 20.00 32.74 58.64
MIR-098 2988.6 1546.78 1759.25 1017.56 817.07 2528.8 1946.42
MIR-099a 2.15 4.33 4.78 2.56 0.62 9.21 0.00
MIR-099b 390.87 20.36 267.57 98.02 121.27 513.83 11.88
MIR-100 74.40 183.60 181.89 516.16 290.00 911.31 0.00
MIR-101-1 0.00 2.14 0.00 0.00 0.00 0.79 8.26
MIR-101-2 83.67 36.37 46.66 12.11 6.35 11.27 27.78
MIR-103-1,2 1736.8 1173.50 911.28 1543.24 388.24 2042.4 988.41
MIR-105-1,2 0.00 4.98 1.01 0.00 1.53 1.40 1.66
MIR-106a-P 0.00 0.84 0.00 0.00 0.00 0.00 0.00
MIR-106b 643.23 365.45 155.34 1217.84 112.89 505.86 374.02
MIR-107 2599.9 1795.67 1130.08 2155.88 595.79 3071.9 1141.60
MIR-108 24.67 22.08 29.42 21.71 23.79 24.28 0.00
MIR-122a 0.00 0.00 0.00 0.00 0.00 0.00 0.00
MIR-124a-1,2,3 48.37 28.91 9.75 0.84 0.79 133.38 2.36
MIR-125a 153.37 43.12 85.41 36.28 94.69 180.34 6.87
MIR-125b-1 34.04 77.81 98.61 65.13 95.08 252.57 10.26
MIR-125b-2 0.00 0.00 0.74 11.66 0.00 13.43 0.00
MIR-126 1919.2 398.62 356.44 391.31 323.62 341.22 20.25
MIR-127 58.85 32.27 81.48 13.06 78.19 46.30 170.98
MIR-128a 283.02 74.01 123.25 41.73 23.87 104.80 395.36
MIR-128b 1.72 0.60 1.90 350.06 5.73 2.24 3.68
MIR-129-2 14.43 7.05 14.11 9.73 29.07 19.58 46.35
MIR-130a-P 27.95 19.33 29.53 6.84 14.95 11.74 1.75
MIR-130b 0.00 12.74 23.73 12.67 12.33 13.76 5.86
Continued
299
Table C.3. continued
MIR-132 14.41 25.06 33.16 10.68 17.16 30.97 24.00
MIR-133a-1,2 17.50 32.24 33.68 10.26 16.37 27.26 188.07
MIR-133b 0.00 18.66 4.56 4.91 0.00 0.00 77.50
MIR-134 61.16 9.98 21.90 5.95 2.86 53.70 96.61
MIR-135a-1,2 55.93 22.03 31.35 55.86 16.96 440.41 199.79
MIR-135b-P 18.35 31.83 85.99 13.60 32.54 13.72 24.45
MIR-136 38.94 17.52 15.16 1.90 0.00 113.15 11.63
MIR-137* 0.00 0.00 0.00 0.00 0.00 1.50 0.00
MIR-138-1,2 9.46 0.00 11.27 0.00 0.00 0.00 0.00
MIR-139 33.06 38.28 45.56 51.89 30.19 30.16 20.55
MIR-140 1.28 0.95 2.06 0.78 0.75 0.83 8.87
MIR-141 0.00 55.91 7.05 8.80 2.98 13.43 52.01
MIR-142-P 272.27 22.74 10.50 13.85 56.46 1.27 1067.31
MIR-143 111.53 86.95 74.34 14.06 14.13 74.32 2.21
MIR-144 0.00 12.48 0.00 0.00 0.00 0.00 13.24
MIR-145-P 542.91 53.48 557.61 19.20 13.76 146.10 2.31
MIR-146 0.98 0.00 2.60 0.00 0.00 0.00 0.00
MIR-147 21.87 11.06 20.47 3.33 11.25 1.28 14.02
MIR-148a-P 0.00 0.00 0.00 0.00 0.00 0.00 0.00
MIR-148b-P 117.13 32.24 73.32 0.00 0.00 29.58 4.96
MIR-149 7.60 14.08 35.69 181.88 4.92 115.10 5.76
MIR-150 69.00 12.71 9.17 104.88 1.28 11.57 86.41
MIR-151 23.13 15.29 5.20 1.19 4.71 14.31 21.16
MIR-152 51.36 27.49 41.70 16.87 53.88 238.29 14.63
MIR-153-1,2 8.37 4.55 9.29 2.09 0.00 21.57 16.16
MIR-154 14.18 13.78 13.18 12.76 0.00 24.64 0.00
MIR-155 571.36 76.52 155.47 387.04 16.45 98.76 162.46
MIR-181a,c 229.72 103.91 55.70 270.13 141.25 87.35 10.32
MIR-181b-1,2 1.90 0.00 1.04 5.51 0.00 11.20 0.00
MIR-182 0.00 61.16 108.60 139.94 8.52 709.30 16.64
MIR-183 2.56 1.64 2.64 1.80 5.40 11.11 0.00
MIR-184 4.20 4.00 2.67 2.02 1.40 54.33 8.18
MIR-185 22.82 46.63 33.84 17.61 29.95 43.50 74.87
MIR-186-P 465.19 192.78 407.65 39.92 37.49 284.16 515.35
MIR-187 0.00 0.00 0.00 0.00 0.00 0.00 2.50
MIR-188b-P 0.00 2.23 0.00 0.00 0.00 0.00 0.00
MIR-190 0.00 0.00 0.00 0.00 0.00 0.00 0.00
MIR-191 2.48 0.00 0.00 0.00 0.00 0.00 15.83
MIR-192 0.00 0.00 2.38 0.00 0.00 0.00 0.00
MIR-193 27.32 14.14 26.48 6.42 22.02 10.41 7.84
MIR-194-1,2 2.19 0.76 0.78 0.92 0.00 1.30 2.65
Continued
300
Table C.3. continued
MIR-195-P 14.76 5.11 25.34 11.35 12.76 8.37 45.97
MIR-196a-1 0.00 0.58 0.00 1.95 0.00 3.02 7.77
MIR-196a-2 9.53 0.00 11.48 0.71 8.02 22.46 0.00
MIR-196b 59.01 18.36 72.26 2968.06 0.00 794.75 12.63
MIR-197 170.72 78.22 65.19 24.08 8.50 95.37 456.62
MIR-198 0.00 0.00 0.00 0.00 0.00 0.00 0.00
MIR-199a-1-P 13.40 4.19 8.59 13.66 3.46 5.24 51.66
MIR-199a-2-P 180.20 26.78 45.31 10.04 3.48 24.68 12.75
MIR-199b-P 26.09 3.93 30.15 13.55 11.06 40.21 44.58
MIR-200a-P 211.03 272.04 374.90 180.80 121.46 279.70 493.96
MIR-200b 1.84 56.75 76.41 8.36 15.43 0.00 6.50
MIR-200c 0.00 10.07 10.45 0.00 9.58 0.00 12.83
MIR-203 8.52 20.82 0.62 1.69 16.64 21.52 0.00
MIR-204 20.22 0.00 12.65 1.88 3.91 13.81 1.70
MIR-205 0.00 0.00 228.76 82.69 0.00 4.25 0.00
MIR-206 0.00 0.00 0.00 43.22 0.00 10.70 0.00
MIR-208 3.30 0.00 1.10 0.00 1.96 0.00 4.33
MIR-210 47.39 99.78 196.22 232.78 22.58 196.01 39.17
MIR-211 18.55 5.24 23.11 30.31 18.01 16.89 54.18
MIR-212 84.07 237.55 288.11 202.56 57.46 280.21 20.46
MIR-213 23.78 20.15 0.00 204.50 0.00 25.67 51.34
MIR-214 5.06 2.70 8.20 13.24 2.52 13.29 14.31
MIR-215 28.37 31.00 22.70 30.58 14.29 34.56 125.74
MIR-216 0.00 0.00 0.00 1.75 1.02 0.00 3.41
MIR-217 0.00 0.00 0.00 0.00 0.00 0.00 0.00
MIR-218-1,2 285.05 85.82 164.76 94.98 45.67 243.56 9.99
MIR-219-1,2 1.61 4.87 8.38 6.10 2.38 5.74 8.92
MIR-220 8.98 3.50 20.11 6.10 2.03 133.63 14.91
MIR-221 52.24 23.83 86.11 34.64 37.60 15.59 115.02
MIR-222-P 28.69 0.00 54.51 20.18 10.99 15.30 32.35
MIR-223 20.20 0.00 3.26 0.00 0.00 0.00 33.32
MIR-224 19.96 59.41 24.60 19.73 6.86 27.13 2.10
MIR-296 11.38 0.00 11.37 11.56 0.69 16.04 3.08
MIR-299-P 7.79 5.04 0.00 9.27 9.65 8.45 52.46
MIR-301 26.16 44.44 20.75 208.64 10.46 34.45 17.26
MIR-302 7.47 7.28 5.80 4.20 14.16 12.79 48.90
MIR-302b 15.67 10.36 10.31 21.27 11.14 10.35 28.78
MIR-302c 1.13 0.00 1.69 0.00 0.00 0.00 4.90
MIR-302d 0.00 0.00 0.00 0.00 0.00 1.62 0.00
MIR-320 9.24 32.28 8.39 10.97 9.42 30.28 5.26
MIR-321 3499.4 1971.63 2990.43 1050.89 1658.58 3033.7 577.39
Continued
301
Table C.3. continued
MIR-323 19.49 5.51 8.25 10.28 3.34 42.48 6.52
MIR-324-P 29.07 68.12 53.56 21.95 51.69 52.45 184.79
MIR-325 0.00 10.03 6.66 47.78 7.18 2.35 0.00
MIR-326-P 22.11 8.00 9.19 1.19 0.00 1.37 0.00
MIR-328 6.81 4.06 8.08 2.19 0.62 7.32 18.43
MIR-330 20.40 16.26 7.47 20.44 6.31 4.95 6.41
MIR-331-P 53.65 9.67 135.36 21.64 103.69 12.88 53.83
MIR-335 0.00 10.09 0.00 0.00 0.00 0.00 2.57
MIR-337 0.00 0.00 0.00 7.00 0.00 4.73 0.00
MIR-338 8.69 10.85 1.26 0.00 1.30 0.00 0.00
MIR-339 412.13 324.99 315.69 238.68 269.71 348.19 405.80
MIR-340 482.21 276.93 445.17 154.46 100.26 488.53 174.58
MIR-342 101.21 38.81 20.73 178.57 0.00 9.98 54.56
MIR-345 7.58 13.57 35.72 10.19 13.19 4.70 38.72
MIR-346 89.10 68.15 103.79 77.76 77.72 60.60 123.37
MIR-361 3.91 5.06 12.24 8.23 6.52 5.91 43.85
MIR-367 3.42 1.07 0.97 1.49 2.16 1.09 6.72
MIR-368 2.79 0.00 1.73 5.49 0.00 14.60 0.00
MIR-369 7.58 0.00 0.00 7.95 2.06 3.57 6.25
MIR-370 198.22 55.97 39.50 23.50 12.07 323.43 0.00
MIR-371 7.05 0.00 0.00 0.00 0.00 11.53 8.21
MIR-372 0.00 0.00 0.00 0.00 0.00 0.00 0.00
MIR-373 14.31 0.00 1.50 0.00 1.25 0.00 2.38
MIR-374-P 0.00 0.00 0.95 0.00 0.86 0.65 2.74
MIR-375 0.00 31.53 17.04 0.00 1.30 34.96 0.00
MIR-376 33.75 0.00 8.22 10.55 5.26 36.88 6.08
MIR-377 8.01 0.00 15.80 0.00 0.00 10.59 0.00
MIR-378 0.00 0.00 0.00 0.00 0.00 0.00 0.00
MIR-379 147.88 25.66 69.91 51.20 0.00 276.33 0.00
MIR-380 43.97 11.61 39.43 30.73 0.00 36.36 0.00
MIR-381 0.00 0.00 0.00 0.00 0.00 0.00 0.00
MIR-382 13.42 0.00 2.88 0.00 0.00 8.88 6.15
MIR-383 0.00 0.00 1.80 2.49 1.52 1.69 1.10
MIR-384 0.00 0.00 0.00 0.00 0.00 0.00 0.00
MIR-422b 0.00 2.02 0.60 0.00 0.82 3.37 0.00
MIR-423 16.38 12.02 9.16 8.49 10.03 11.99 32.23
MIR-424 8.90 0.00 0.00 16.17 0.00 59.19 0.00
MIR-425 24.30 15.85 21.78 9.17 0.00 95.41 314.06
302
miRNA Adipose Bladder Brain Cervix Colon Heart Lung Liver
hsa-let-7a 10613.6 13363.8 11789.5 19063.4 7767.6 8369.8 11502.0 3382.1
hsa-let-7b 53686.2 58352.8 41012.0 98751.3 24511.0 16205.2 46432.0 11378.6
hsa-let-7c 15171.5 24047.5 18520.0 59273.5 4039.5 9002.8 8229.3 5076.9
hsa-let-7d 463.0 1577.2 1288.6 1405.7 868.4 1002.0 1286.1 269.1
hsa-let-7e 177.4 870.9 837.0 799.3 445.3 387.1 424.4 98.8
mmu-let-7f 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0
hsa-let-7g 14248.8 27606.9 18884.3 28180.2 14598.9 12733.3 15906.0 5263.4
hsa-let-7i 1833.3 3157.4 1586.6 3235.7 1367.8 1307.6 1755.2 306.8
hsa-miR-001 9109.7 63472.5 1325.4 17524.0 19352.9 329801.5 1615.6 110.5
hsa-miR-007 148.0 358.4 6796.9 258.3 1548.2 185.4 251.4 24.9
hsa-miR-009 447.3 1362.2 259711.7 5183.9 1958.8 734.3 287.3 100.6
hsa-miR-009* 175.8 347.5 59637.3 1009.5 467.1 141.6 53.7 32.0
hsa-miR-010a 3456.7 2843.5 178.8 7577.7 9972.8 846.7 4004.1 1117.6
hsa-miR-010b 1955.6 2675.7 1.6 5923.1 3506.6 439.6 655.5 84.6
hsa-miR-015a 1469.0 2809.8 2106.9 1700.8 1911.6 1290.5 2697.0 592.9
hsa-miR-015b 7599.0 13804.4 4762.4 10478.2 10013.2 7506.7 16577.5 3021.3
hsa-miR-016 23401.5 45700.8 22086.4 33104.2 23722.6 19664.9 34670.9 4660.6
hsa-miR-017-3p 75.3 249.2 103.2 161.9 129.7 57.9 71.1 33.1
hsa-miR-017-5p 907.5 2031.1 1124.0 1920.1 3358.2 1453.4 1701.9 1352.3
hsa-miR-018 59.6 148.6 63.7 88.5 263.0 77.7 172.4 83.3
hsa-miR-019a 4266.5 12744.0 5282.6 11758.0 18071.8 6834.0 10696.8 7416.0
hsa-miR-019b 31714.8 85010.3 33748.2 72824.1 92091.2 38935.5 64131.5 43563.2
hsa-miR-020 39732.0 95865.6 39963.5 67326.7 92793.7 41833.1 67678.4 41971.9
hsa-miR-021 26741.7 64947.7 4647.8 41063.0 55004.4 14811.2 72170.0 10514.9
hsa-miR-022 2932.6 3534.5 1178.6 2001.7 1482.6 4687.4 1309.6 1090.6
hsa-miR-023a 504.8 969.0 124.3 796.6 458.8 720.5 562.6 65.3
hsa-miR-023b 745.7 8038.1 3460.0 7083.1 4230.6 6009.6 3244.9 2064.0
hsa-miR-023bN 12333.9 61401.4 20958.3 52388.3 26920.6 96699.1 15497.5 17540.4
hsa-miR-024 120059.2 415038.3 87669.7 377404.3 205009.3 406258.4 229790.8 68404.9
hsa-miR-025 3006.7 4079.1 2281.9 4470.8 2686.5 1822.5 3062.5 1185.3
hsa-miR-026aN 129641.3 252327.8 142130.1 346478.5 130102.6 126069.0 142534.4 35995.3
hsa-miR-026b 47027.8 92977.3 26848.6 92781.3 37773.2 46607.2 46037.5 21980.7
hsa-miR-026bN 35087.9 68284.4 20878.7 68247.7 28734.4 31876.8 34435.5 15203.7
hsa-miR-027a 25238.1 43315.8 3762.6 39226.1 25347.8 28601.6 23923.0 3136.4
hsa-miR-027b 3825.6 27048.3 6982.0 17730.8 10084.8 16640.2 5480.0 3537.9
hsa-miR-027bN 40027.2 203039.0 69936.8 163833.7 82223.1 122599.9 63230.6 40280.4
hsa-miR-028 5384.3 18487.0 1583.6 11186.5 10903.8 6515.5 4118.1 2529.9
hsa-miR-029a 12067.4 40038.3 20879.1 46124.3 19275.5 19710.3 22755.2 6667.7
hsa-miR-029b 155.8 912.4 704.7 705.0 712.5 682.7 768.8 235.2
hsa-miR-029c 3901.0 13766.6 5738.9 10038.4 12226.1 11286.7 10195.7 2282.9
hsa-miR-030a-3p 11168.8 13205.0 8839.6 8944.4 5340.5 16837.6 13483.9 3227.8
hsa-miR-030c 54329.4 96796.9 71410.0 82185.6 53298.5 182162.9 66838.1 35011.5
Continued
Table C.4. Mature microRNA expression in 22 different normal human tissues and
cells. All data was generated using real-time PCR and TaqMan microRNA assays
(Applied Biosystems). Data is presented as copies of microRNA per cell.
303
Table C.4. continued
hsa-miR-030d 8337.8 15915.5 5934.4 11902.9 8795.8 19264.4 18899.8 4098.8
hsa-miR-030e-3p 2010.1 4862.3 3418.0 4468.2 3215.0 11594.2 2399.2 2989.7
hsa-miR-031 2073.0 13163.9 5489.7 14518.5 24363.7 257.6 3025.4 155.3
hsa-miR-032 984.4 2165.0 1389.1 1764.9 1608.9 1899.1 2047.1 1062.9
hsa-miR-033 0.0 0.0 8.0 23.7 0.0 0.0 20.0 8.4
hsa-miR-034a 65.1 420.7 262.5 448.2 114.5 194.0 637.5 143.0
hsa-miR-034aN 1677.3 5991.5 2872.9 5505.9 1953.6 2572.7 5635.5 1573.6
hsa-miR-034b 5.7 13.6 31.2 72.6 8.6 0.0 785.5 0.0
hsa-miR-034b 0.0 15.8 39.0 26.3 33.3 9.9 829.7 0.0
hsa-miR-092N 19228.1 32751.1 25649.4 35311.3 30903.8 24967.7 23609.6 21977.0
hsa-miR-093 15068.3 34559.4 18272.4 17943.1 27656.5 12782.2 23722.9 7257.9
hsa-miR-095 1564.2 1542.3 6938.8 1222.8 1460.5 2261.2 1063.6 272.2
hsa-miR-096 468517.2 639331.1 782408.6 481715.0 389223.7 515124.3 378240.7 390082.2
hsa-miR-098 613.1 923.3 2036.6 870.1 563.3 642.2 708.8 198.2
hsa-miR-099a 15038.0 51060.1 44826.2 87244.3 12820.7 22632.7 16840.6 6074.1
hsa-miR-099b 3098.7 7570.8 9293.8 9091.4 3670.2 6916.7 4356.8 568.6
hsa-miR-100 10345.8 32294.3 27832.9 50040.3 8073.8 13291.2 11666.9 4477.4
hsa-miR-101 80.4 498.8 81.7 672.6 251.4 273.8 455.6 181.4
hsa-miR-103 3061.9 4921.9 9048.6 5119.5 3163.5 3186.7 4475.3 1760.1
hsa-miR-105 641.3 1024.4 759.3 1125.1 799.4 546.9 656.3 564.2
hsa-miR-106a 45983.6 94030.2 105822.5 58176.8 38763.9 66565.9 59599.1 67641.6
hsa-miR-106b 2435.1 7617.3 2762.7 5292.2 6471.7 2673.9 4415.2 1362.8
hsa-miR-107 5.4 23.9 108.4 0.0 29.8 51.3 9.7 34.0
hsa-miR-108 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0
hsa-miR-122a 1.5 0.0 0.0 1.7 1.8 0.0 0.0 4135.6
hsa-miR-124a 0.0 0.0 4859.2 0.0 0.0 0.0 0.0 0.0
hsa-miR-125a 50192.1 93609.2 90938.5 126526.5 44090.2 66642.5 59930.1 10285.3
hsa-miR-125b 47325.8 109389.7 124707.2 212464.6 27670.1 57528.3 26785.3 11550.6
hsa-miR-126 217325.4 279589.6 91681.9 248430.4 123410.2 278539.3 445485.7 75287.0
hsa-miR-126* 19034.3 32416.8 9665.8 33397.8 15968.7 32268.2 43612.7 8614.8
hsa-miR-127 1251.4 4901.2 24597.2 19442.5 3838.5 3788.0 2517.6 1124.4
hsa-miR-128a 0.0 0.0 112.5 8.5 0.0 0.0 0.0 0.0
mmu-miR-129-2 0.0 3.6 119.5 0.0 0.0 0.0 0.0 0.0
hsa-miR-130a 226.4 2195.3 711.7 5060.9 1695.2 1792.5 2534.8 607.2
hsa-miR-130aN 3544.7 13294.9 3116.4 26032.9 6835.0 6236.2 13324.3 2273.4
hsa-miR-130b 18.2 82.4 78.1 123.2 119.1 102.5 93.9 159.3
hsa-miR-132 2478.2 9980.9 48090.7 7490.7 7211.4 6003.6 5579.4 825.3
hsa-miR-133a 894.8 9360.7 259.4 2386.2 3162.2 84221.7 242.3 29.4
hsa-miR-134 184.8 448.8 1803.0 1501.0 295.9 211.8 216.4 131.8
hsa-miR-135a 28.5 1134.0 4844.5 2949.6 764.9 638.7 657.1 18.1
hsa-miR-135b 285.6 682.6 681.7 2933.3 1367.2 146.5 5183.0 165.1
mmu-miR-137 0.0 45.2 3798.6 93.5 107.7 18.6 10.6 0.0
hsa-miR-139 598.4 557.2 1700.1 364.6 255.7 288.7 121.9 129.4
hsa-miR-140 12329.2 26575.0 15428.3 36322.4 15781.1 18077.5 22344.8 4913.8
hsa-miR-141 140.5 993.3 7.6 1517.9 5994.4 0.0 3630.2 33.1
hsa-miR-142-3p 3258.8 18481.3 3017.4 12062.2 30539.7 6799.8 53195.0 7053.9
Continued
304
Table C.4. continued
hsa-miR-142-5p 337.0 2397.5 513.7 1605.4 2077.5 852.7 5154.0 947.6
hsa-miR-143 796.5 34165.5 411.2 15192.9 15694.5 2589.7 2430.7 367.9
hsa-miR-145 2395.0 30948.1 991.1 19029.5 16320.9 6585.8 2870.3 605.9
hsa-miR-146 14186.4 14772.1 6301.9 13472.5 19605.1 7012.8 26077.3 5830.6
hsa-miR-146b 13234.0 22446.6 9139.1 12031.0 21328.1 14911.7 93709.4 16764.5
hsa-miR-147 1.4 2.9 3.5 4.3 5.0 1.4 3.7 3.0
hsa-miR-148a 1337.0 5520.8 518.6 12745.5 8256.2 3829.5 6607.8 17951.9
hsa-miR-148b 292.7 1520.6 1279.1 1381.7 965.1 969.6 1347.7 567.3
hsa-miR-149 741.5 2623.2 35807.8 5439.7 1646.5 3171.8 719.2 186.5
hsa-miR-150 59978.4 48226.4 10449.2 49111.8 55927.0 26178.5 67589.9 4750.5
hsa-miR-152 10710.5 16574.7 3196.3 21839.2 7491.9 11178.7 5510.0 3199.8
hsa-miR-152N 7113.8 12652.8 2671.9 13593.6 6786.2 9783.6 4485.2 2768.0
hsa-miR-153 7.0 17.9 311.8 6.2 23.4 7.7 0.0 0.0
hsa-miR-154 0.0 0.0 2.9 7.3 0.0 0.0 0.0 0.0
hsa-miR-154* 0.0 0.0 119.4 42.4 15.5 0.0 0.0 11.0
hsa-miR-155 2127.3 3234.0 1011.6 2726.7 5350.1 1647.3 8906.0 413.7
hsa-miR-181a 907.5 1008.2 17247.3 2207.4 1365.9 1734.9 4932.3 206.7
hsa-miR-181b 2516.9 2808.2 55383.1 3941.3 3022.0 3878.3 8753.6 664.4
hsa-miR-181c 67.1 158.9 1172.6 286.2 158.5 140.9 292.7 22.4
hsa-miR-182 3.6 11.9 14.6 51.5 16.7 8.4 0.0 4.4
hsa-miR-183 47.8 69.7 17.3 103.2 98.9 6.0 184.8 1.8
hsa-miR-184 12.2 50.3 312.8 339.6 30.6 60.4 517.1 0.0
hsa-miR-186 6979.8 14940.1 5488.1 12654.6 8355.5 10295.0 7731.1 3003.7
hsa-miR-187 25.6 1229.3 205.1 1102.2 269.8 134.8 224.0 13.2
hsa-miR-188b 80.8 121.6 74.6 138.8 60.8 46.0 129.7 67.0
hsa-miR-190 180.1 253.9 345.8 361.1 208.7 291.7 64.8 15.5
hsa-miR-191 18254.3 30003.3 30378.0 31012.1 21922.1 16138.0 23827.8 7258.0
hsa-miR-192 875.1 3543.3 1810.0 2619.0 244571.9 933.8 2124.9 62253.7
hsa-miR-193 884.6 1500.6 199.6 1081.9 709.2 612.6 464.6 418.9
hsa-miR-194 199.0 932.0 669.5 961.3 94041.3 446.3 742.4 27056.9
hsa-miR-195 49023.9 92401.5 20025.7 128740.6 43277.1 29919.6 36915.9 6221.7
hsa-miR-196a 4767.5 1834.0 0.0 3141.2 2392.3 2.2 10.4 0.0
hsa-miR-196b 313.3 2195.7 0.0 14603.8 2739.1 0.0 0.0 0.0
hsa-miR-197 4561.6 7734.1 13720.7 11449.0 4787.0 14952.1 5190.7 1622.4
hsa-miR-199a 189.0 331.5 27.6 502.5 153.2 91.2 110.6 20.3
hsa-miR-199a* 15167.1 28938.3 507.1 44754.8 12253.4 9120.7 10163.9 2151.8
hsa-miR-199b 201.6 289.3 10.9 572.6 72.2 41.9 157.9 11.4
hsa-miR-199-s 810.2 2325.5 13.5 2946.8 1060.6 589.0 701.0 258.1
hsa-miR-200a 853.0 3753.7 284.4 7563.1 25830.3 14.9 6584.2 911.0
hsa-miR-200b 1077.4 2117.7 119.3 7187.6 41428.6 7.7 4732.8 691.4
hsa-miR-200bN 1847.4 5569.1 358.3 10300.1 34706.6 9.8 6904.3 1060.1
hsa-miR-200c 15013.1 21230.7 210.1 38007.7 88045.1 106.6 56393.3 595.5
hsa-miR-200cN 8158.9 12275.5 76.7 24293.0 74780.9 17.8 40231.9 246.4
hsa-miR-203 9762.5 3695.6 1911.0 19958.2 8302.2 448.1 9223.2 884.5
hsa-miR-204 2878.6 7813.0 21112.5 11857.7 1233.6 1945.0 628.0 964.5
hsa-miR-205 4460.3 4632.7 14.8 8029.1 3.1 0.0 1187.9 0.0
Continued
305
Table C.4. continued
hsa-miR-206 3763.6 4413.2 31.2 192.2 95.2 16.0 9.2 2.6
hsa-miR-208 0.0 0.0 0.0 9.4 0.0 80.7 0.0 0.0
hsa-miR-210 225.1 1285.8 551.0 963.5 1275.1 1755.3 1375.8 485.2
hsa-miR-210N 929.6 3261.5 1640.9 2903.1 3385.0 4306.0 3653.1 1416.9
hsa-miR-211 130.6 9.0 856.2 477.4 7.0 3.6 8.4 11.0
hsa-miR-212 1185.6 1823.5 3837.4 1208.4 960.1 1221.4 1206.9 305.3
hsa-miR-213 9.2 15.7 468.9 20.6 37.7 47.8 69.7 2.8
hsa-miR-214 23094.4 43803.0 640.2 54476.8 19565.1 13524.7 12215.7 3942.7
hsa-miR-215 159.3 125.7 4.4 10.8 42464.7 18.2 4.0 85.7
hsa-miR-216 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0
hsa-miR-217 0.0 7.2 3.7 0.0 0.0 0.0 0.0 0.0
hsa-miR-218 4346.8 16850.1 22899.5 25310.3 5397.8 5478.5 11158.6 424.5
hsa-miR-219 0.0 0.0 272.2 0.0 0.0 0.0 0.0 0.0
hsa-miR-220 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0
hsa-miR-221 2923.7 10950.7 15420.3 5815.5 2840.5 11868.7 3330.3 347.3
hsa-miR-222 10729.8 36594.8 47049.5 31550.2 12310.6 23973.3 16077.2 1229.9
hsa-miR-223 44405.6 103591.4 35812.0 77032.5 91189.0 61790.4 368144.1 35692.1
mmu-miR-224 198.6 52.9 0.0 99.8 13.4 99.0 44.0 9.5
hsa-miR-296 302.0 825.8 422.3 1137.2 386.7 767.8 356.2 95.0
hsa-miR-299 0.0 0.0 0.0 7.7 0.0 0.0 0.0 0.0
hsa-miR-301 36.2 390.3 329.8 365.7 347.3 395.1 456.6 48.6
hsa-miR-302a 0.0 0.0 23.6 0.0 0.0 302.1 0.0 0.0
hsa-miR-302a* 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0
hsa-miR-302b 14.5 607.7 713.5 554.9 1808.2 1899.7 1218.9 875.2
hsa-miR-302c 0.0 0.0 39.9 0.0 0.0 277.1 0.0 0.0
hsa-miR-302c* 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0
hsa-miR-302d 0.0 2.9 29.1 5.2 2.0 1073.7 1.7 0.0
hsa-miR-320 11807.7 22151.8 9290.0 25780.9 13686.5 11110.1 5840.9 6033.3
hsa-miR-321 26330.9 70277.0 58569.1 41675.5 47108.8 41927.6 42983.2 44266.8
hsa-miR-323 4.9 4.3 4197.3 20.8 26.0 3.4 4.0 0.0
hsa-miR-324-3p 923.1 6344.4 6447.7 5612.4 3059.5 2398.5 3304.4 713.4
hsa-miR-324-5p 777.9 2767.0 5829.1 2047.6 1620.5 1522.6 1626.2 500.3
hsa-miR-325 5.2 5.6 9.8 1.8 7.3 16.4 4.8 2.8
hsa-miR-326 7.1 19.0 71.2 8.8 19.2 7.5 39.7 3.4
hsa-miR-328 4159.3 7195.1 31722.8 7872.9 2438.4 5631.1 3472.1 1121.2
hsa-miR-330 49.8 150.4 1879.9 75.1 69.9 55.8 83.9 11.0
hsa-miR-331 6533.2 19854.1 22978.2 12182.3 9275.6 12259.5 10546.8 3279.8
hsa-miR-335 4309.0 3070.6 3439.4 2706.9 1838.2 1414.7 3160.1 676.8
hsa-miR-337 0.0 0.0 11.3 0.0 0.0 3.5 0.0 0.0
hsa-miR-338 1.6 18.5 384.8 15.1 92.7 23.1 68.7 10.9
hsa-miR-339 98.9 163.6 88.4 160.9 118.8 55.0 134.1 15.9
hsa-miR-340 437.8 499.0 2348.8 406.4 299.1 524.7 423.2 285.7
hsa-miR-342 3234.3 9529.3 33471.6 9353.6 8580.5 6893.3 17643.4 3869.9
hsa-miR-345 2255.5 2682.4 2340.2 3443.6 1819.1 2049.9 2116.6 1055.3
hsa-miR-346 26.5 22.4 1509.1 68.0 21.8 34.1 31.4 4.7
hsa-miR-361 1213.2 2362.1 2507.0 3358.8 1366.4 1495.7 1108.2 412.4
Continued
306
Table C.4. continued
hsa-miR-367 0.0 0.0 0.0 0.0 0.0 186.9 0.0 0.0
hsa-miR-368 0.0 0.0 0.0 20.2 0.0 8.2 0.0 0.0
hsa-miR-369 0.0 0.0 26.4 10.4 3.5 0.0 0.0 0.0
hsa-miR-370 279.6 551.7 6234.0 1451.8 429.6 205.4 262.4 183.5
hsa-miR-371 0.0 18.4 1.9 0.0 2.3 1.7 0.0 0.0
hsa-miR-372 5.8 13.3 7.9 8.7 0.0 13.1 9.1 7.9
hsa-miR-373 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0
hsa-miR-374 5440.2 20949.5 9032.8 20420.9 13029.0 12010.1 12554.7 5028.7
hsa-miR-375 487.3 1796.3 369.3 5491.6 26234.0 7.4 10114.3 2866.0
hsa-miR-376a 213.6 506.5 1163.4 1518.3 315.7 380.2 270.6 147.6
hsa-miR-377 0.0 4.8 7.0 7.9 2.4 4.2 0.0 0.0
hsa-miR-378 852.5 1099.9 129.9 319.1 958.6 2385.2 224.6 361.2
hsa-miR-379 216.9 286.4 1851.7 1220.9 205.8 201.8 121.1 90.6
hsa-miR-380-3p 0.0 0.0 3.8 1.5 0.0 0.0 0.0 0.0
hsa-miR-380-5p 0.0 28.2 50.7 15.1 13.2 0.0 0.0 0.0
hsa-miR-381 8.9 50.6 56.0 54.8 14.2 42.1 7.0 14.8
hsa-miR-382 458.2 723.6 4505.6 1447.3 360.3 445.8 377.6 112.6
hsa-miR-383 66.3 247.7 3430.7 67.3 250.3 0.0 0.0 43.5
hsa-miR-384 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0
hsa-miR-422b 580.4 1011.3 90.5 355.9 898.9 1149.4 178.2 334.2
hsa-miR-423 455.0 1353.7 1478.0 1907.0 1185.0 1320.1 1488.0 595.0
mmu-miR-424 0.0 0.0 0.0 1.7 0.0 0.0 0.0 0.0
hsa-miR-425 552.8 1149.0 1480.2 1071.3 719.5 574.5 786.9 305.8
miRNA Kidney Esophagus Skeletal Muscle Ovary Pancreas Placenta Prostate
hsa-let-7a 10077.2 10852.6 13532.3 25797.4 6375.2 6828.2 11414.2
hsa-let-7b 29151.7 35202.0 22384.8 112522.3 37467.0 9083.4 55437.8
hsa-let-7c 8611.3 8797.4 5552.5 42372.6 3276.7 1701.0 39946.0
hsa-let-7d 659.4 1055.9 1223.3 1118.9 293.9 344.8 1087.9
hsa-let-7e 486.1 500.3 195.5 943.8 124.0 486.7 545.6
mmu-let-7f 0.0 0.0 0.0 0.0 0.0 0.0 0.0
hsa-let-7g 13697.7 15929.7 15021.4 24498.5 6535.3 5644.2 18961.1
hsa-let-7i 1200.0 1604.7 1377.8 2466.0 447.0 764.6 2028.6
hsa-miR-001 809.4 67661.9 1279818.8 10321.6 591.6 1580.3 40853.4
hsa-miR-007 197.0 303.1 89.9 115.1 6013.2 438.4 242.0
hsa-miR-009 1914.8 1596.0 450.7 3437.1 443.7 573.4 1950.2
hsa-miR-009* 439.0 450.5 126.7 654.0 114.0 172.1 521.1
hsa-miR-010a 14974.1 5618.0 1356.9 5150.6 2578.0 776.7 2557.9
hsa-miR-010b 12669.9 1550.7 2195.8 12014.8 346.2 217.8 2358.1
hsa-miR-015a 1268.6 1836.0 2542.1 1548.0 662.8 2343.7 1893.9
hsa-miR-015b 7402.6 9439.1 6141.3 13089.9 3486.1 15774.0 7981.5
hsa-miR-016 15483.3 28366.5 30877.0 34598.9 5415.0 35495.9 37301.7
hsa-miR-017-3p 97.5 126.1 59.3 237.2 40.1 76.1 149.8
hsa-miR-017-5p 2007.9 1770.9 1125.5 1708.2 470.7 2618.5 1776.5
hsa-miR-018 48.7 137.9 39.9 60.9 60.0 222.4 72.4
Continued
307
Table C.4. continued
hsa-miR-019a 9996.6 11367.2 6829.4 10807.9 5883.2 10765.5 10843.4
hsa-miR-019b 53795.0 66636.8 42202.9 73471.4 31443.4 66866.9 73188.9
hsa-miR-020 64255.4 85026.6 49096.9 78862.7 23741.2 77963.5 63874.3
hsa-miR-021 34629.9 48966.0 12954.5 36211.3 14739.6 43467.8 67656.1
hsa-miR-022 1529.2 1887.2 7995.9 1424.4 464.5 3404.3 2888.2
hsa-miR-023a 432.3 688.4 638.2 560.9 85.8 1322.3 631.5
hsa-miR-023b 3969.9 6327.8 2751.7 5491.9 1578.6 3550.7 6281.4
hsa-miR-023bN 28983.4 48382.4 40848.5 38443.1 8720.7 19340.6 32115.5
hsa-miR-024 160481.7 328445.2 291812.1 253145.5 69317.4 529861.1 403163.3
hsa-miR-025 2258.2 2630.2 2466.4 3937.8 1259.3 3653.9 2536.2
hsa-miR-026aN 151615.3 167946.2 179909.3 354910.3 49692.7 107719.7 212595.6
hsa-miR-026b 52285.7 51517.9 45431.2 75479.5 20646.2 69269.9 72012.2
hsa-miR-026bN 39639.5 38831.0 30671.5 60203.7 15160.8 57317.5 60006.1
hsa-miR-027a 18602.1 40746.0 37020.8 19222.5 5523.9 66255.5 33213.1
hsa-miR-027b 8606.2 19131.4 9358.3 12034.9 2810.4 7210.3 23503.6
hsa-miR-027bN 85127.9 147151.2 71229.8 115354.7 43815.3 77943.7 200770.2
hsa-miR-028 3194.7 17191.3 5864.5 7171.9 1472.6 3582.7 10413.5
hsa-miR-029a 27559.5 22459.9 35949.6 30619.2 10146.5 17298.4 32934.6
hsa-miR-029b 655.2 876.6 1201.0 413.5 345.7 618.0 564.1
hsa-miR-029c 10731.8 10086.8 12606.5 7969.7 9136.2 3501.8 13267.5
hsa-miR-030a-3p 38859.0 11564.7 17926.4 8570.8 8775.9 21285.2 10377.2
hsa-miR-030c 232146.8 77726.7 108072.7 77643.7 35355.1 82507.2 64814.7
hsa-miR-030d 21134.6 11119.3 19057.6 11524.2 6671.0 42985.5 12327.1
hsa-miR-030e-3p 2705.1 3864.6 7609.2 5815.4 1777.2 1646.9 3516.1
hsa-miR-031 10786.1 20549.9 191.1 5520.9 1183.5 6564.3 15207.5
hsa-miR-032 3166.7 1862.4 1760.5 2262.8 1217.2 2969.5 1856.0
hsa-miR-033 43.2 21.6 0.0 0.0 0.0 0.0 4.4
hsa-miR-034a 196.5 294.8 100.6 651.1 160.1 207.0 640.3
hsa-miR-034aN 2318.0 4433.9 1460.1 6753.0 2307.6 2615.5 6223.0
hsa-miR-034b 0.0 38.7 0.0 699.6 9.2 72.9 23.6
hsa-miR-034b 0.0 21.2 0.0 564.8 0.0 62.1 30.5
hsa-miR-092N 26746.7 30797.3 33938.5 56089.7 13585.8 22934.4 22135.0
hsa-miR-093 12930.1 21802.2 11116.9 19528.7 3984.0 35180.8 21769.4
hsa-miR-095 2089.9 3895.8 65316.6 1276.1 926.6 516.1 2257.0
hsa-miR-096 513477.8 624367.1 508885.3 377259.3 866530.1 585776.3 436806.1
hsa-miR-098 992.0 666.0 946.4 979.4 272.5 386.0 661.6
hsa-miR-099a 21757.2 20340.1 10295.2 66443.6 4039.7 36189.8 45368.5
hsa-miR-099b 7743.0 4869.8 1652.5 8065.5 1309.6 9065.5 3968.1
hsa-miR-100 12638.3 14558.9 5442.3 47843.3 2874.4 31058.1 29408.7
hsa-miR-101 351.7 407.2 771.3 554.2 183.3 306.5 545.4
hsa-miR-103 4380.2 3324.3 1566.7 3877.9 800.8 5139.5 3858.6
hsa-miR-105 561.1 600.7 780.9 1454.4 484.9 874.9 830.5
hsa-miR-106a 48954.8 86632.0 104687.1 66511.4 123234.3 75586.7 48471.2
hsa-miR-106b 4108.8 5031.2 2933.2 4837.2 1629.4 6720.4 4213.4
hsa-miR-107 191.1 18.7 24.6 19.8 3.8 16.1 20.1
hsa-miR-108 0.0 0.0 0.0 0.0 0.0 0.0 0.0
Continued
308
Table C.4. continued
hsa-miR-122a 0.0 1.7 0.0 0.0 0.0 3.1 0.0
hsa-miR-124a 0.0 0.0 0.0 0.0 0.0 0.0 0.0
hsa-miR-125a 83936.9 59566.7 21498.7 127804.7 15482.9 126209.9 56673.1
hsa-miR-125b 29566.1 54132.1 31899.8 219811.6 13399.2 35933.5 111118.5
hsa-miR-126 165237.6 185440.2 175040.4 167043.0 44896.8 242580.8 160471.4
hsa-miR-126* 18384.7 22526.1 23500.2 19513.5 5942.3 17413.4 20759.2
hsa-miR-127 2293.7 2213.2 2530.1 14586.1 2624.8 17410.7 6928.7
hsa-miR-128a 0.0 0.0 55.9 0.0 0.0 0.0 0.0
mmu-miR-129-2 0.0 0.0 0.0 15.3 18.8 0.0 9.3
hsa-miR-130a 2976.6 1998.4 462.4 3103.2 897.6 4264.5 3658.9
hsa-miR-130aN 9857.1 9536.9 2427.9 18680.6 3362.4 18147.6 20919.7
hsa-miR-130b 59.3 68.4 40.7 74.9 491.6 303.4 96.0
hsa-miR-132 6702.0 5779.3 4004.6 8949.3 3059.3 2693.8 9394.4
hsa-miR-133a 130.0 16566.8 325436.3 1197.5 67.3 263.8 9766.0
hsa-miR-134 183.3 184.8 210.4 1424.4 257.3 989.5 672.5
hsa-miR-135a 8553.8 381.6 180.9 2348.4 993.9 518.6 5918.2
hsa-miR-135b 4816.5 2086.2 45.1 1990.3 613.2 13871.2 1899.3
mmu-miR-137 0.0 53.9 0.0 17.4 30.0 253.3 53.0
hsa-miR-139 252.1 291.0 201.4 209.3 87.0 141.2 282.9
hsa-miR-140 24294.8 20568.4 19952.9 32248.7 6150.9 15014.3 21493.0
hsa-miR-141 2172.7 1802.4 20.1 421.4 13451.8 6911.1 2594.3
hsa-miR-142-3p 7731.1 15795.7 6664.7 10370.9 5628.6 19655.9 12231.4
hsa-miR-142-5p 751.4 1486.3 689.5 992.2 582.5 1905.4 2023.4
hsa-miR-143 1425.1 20497.1 1682.6 8121.5 538.2 2523.0 19912.3
hsa-miR-145 2130.0 34480.2 1870.8 11749.6 857.6 3829.0 15745.3
hsa-miR-146 14360.1 14523.5 8066.1 6477.8 3699.3 5626.3 13006.3
hsa-miR-146b 20360.1 15067.3 6327.2 33648.9 15368.4 5973.1 20290.1
hsa-miR-147 4.1 3.7 4.7 4.3 4.0 4.0 4.1
hsa-miR-148a 6108.6 4135.1 2462.4 11106.9 68386.1 3507.9 7622.4
hsa-miR-148b 1419.0 1070.6 594.1 853.0 557.7 2028.9 1342.0
hsa-miR-149 1378.5 3223.9 815.1 3076.7 477.5 10109.4 5656.0
hsa-miR-150 19990.7 39163.2 29793.8 20824.6 8550.5 7456.5 24593.9
hsa-miR-152 7578.4 10433.1 12837.4 9650.3 3333.6 8476.2 13787.5
hsa-miR-152N 5980.9 9830.7 9782.3 6787.7 2093.2 7430.4 11156.5
hsa-miR-153 6.5 5.0 20.0 14.1 10.0 0.0 2.5
hsa-miR-154 0.0 0.0 0.0 0.0 0.0 4.0 0.0
hsa-miR-154* 0.0 0.0 4.6 10.8 23.0 79.7 13.3
hsa-miR-155 1773.9 3529.1 1418.5 3979.9 515.6 897.6 2597.6
hsa-miR-181a 3718.4 1043.0 764.9 1591.5 398.9 8781.7 813.9
hsa-miR-181b 6496.3 2433.4 1477.3 5190.6 1359.8 5851.4 2906.4
hsa-miR-181c 251.3 128.1 59.3 178.1 41.5 442.0 181.2
hsa-miR-182 3.7 9.9 7.8 17.5 6.6 7.4 15.5
hsa-miR-183 187.5 228.1 115.6 17.6 170.5 233.8 238.7
hsa-miR-184 243.9 11.7 63.2 9.7 77.4 821.5 88.3
hsa-miR-186 5919.9 8622.7 8137.3 9926.3 2981.2 6981.5 13496.8
hsa-miR-187 627.5 1391.6 0.0 117.3 30.3 0.0 1554.1
Continued
309
Table C.4. continued
hsa-miR-188b 93.2 88.4 100.4 109.9 77.0 92.5 82.8
hsa-miR-190 955.9 113.5 155.4 117.9 73.5 96.7 98.6
hsa-miR-191 18363.5 17658.8 11737.2 31599.3 6565.2 34049.3 22783.2
hsa-miR-192 74764.3 2593.8 1471.9 3087.0 25192.4 4889.5 6287.3
hsa-miR-193 564.7 1020.3 608.9 825.2 405.1 617.4 1182.6
hsa-miR-194 33424.2 931.4 583.5 1130.3 4766.0 1846.8 1577.5
hsa-miR-195 24047.2 48198.7 23501.7 123747.4 10053.2 8734.2 60740.4
hsa-miR-196a 5600.2 97.3 6403.9 3241.0 35.9 800.0 1884.4
hsa-miR-196b 1018.1 0.0 619.3 78.3 0.0 333.8 2756.1
hsa-miR-197 4631.5 4747.1 6150.8 7819.8 1989.1 3278.5 5382.6
hsa-miR-199a 105.0 121.6 45.9 394.6 40.5 233.6 216.4
hsa-miR-199a* 7946.2 11030.4 6974.5 38622.2 3480.8 12258.2 23259.0
hsa-miR-199b 18.0 85.6 85.6 142.5 56.6 118.1 134.5
hsa-miR-199-s 681.0 827.6 382.7 3010.3 300.4 948.2 1590.9
hsa-miR-200a 27531.9 5606.7 54.4 2694.3 14205.7 125.4 9940.1
hsa-miR-200b 20632.3 7950.4 22.9 5605.7 7348.0 37.8 11594.3
hsa-miR-200bN 22674.2 6163.1 60.6 7725.5 13569.1 578.7 17601.6
hsa-miR-200c 26304.8 35266.9 226.5 17442.3 96773.8 100754.4 49800.7
hsa-miR-200cN 15344.7 24488.4 71.8 14630.4 59080.5 73356.0 28981.4
hsa-miR-203 2843.2 104075.3 110.9 2067.3 564.5 17358.7 4062.3
hsa-miR-204 150543.5 1114.1 1135.6 12368.8 1363.1 2951.2 8591.4
hsa-miR-205 90.7 23002.5 22.8 0.0 9.8 3353.5 15420.9
hsa-miR-206 107.1 16885.0 468142.2 97.0 15.8 12.2 27381.7
hsa-miR-208 0.0 0.0 5.1 0.0 0.0 0.0 15.7
hsa-miR-210 1134.9 3106.9 777.8 445.9 159.2 1676.4 720.9
hsa-miR-210N 3026.5 6970.2 1610.5 1316.1 752.5 4835.9 2448.9
hsa-miR-211 238.0 5.7 2.8 6.9 2.3 46.8 11.0
hsa-miR-212 907.8 842.9 838.7 1811.5 651.9 575.7 1716.9
hsa-miR-213 48.3 20.7 0.0 11.2 1.9 151.8 11.2
hsa-miR-214 10752.2 18963.3 8707.0 49907.9 4655.7 9765.2 33002.8
hsa-miR-215 110.3 174.5 18.2 9.7 365.1 5.5 240.5
hsa-miR-216 0.0 0.0 0.0 0.0 999.5 0.0 0.0
hsa-miR-217 16.1 5.8 8.1 0.0 179.8 16.2 0.0
hsa-miR-218 9104.3 10346.9 2029.4 7246.4 2279.8 6245.7 12398.5
hsa-miR-219 0.0 0.0 0.0 0.0 2.9 0.0 0.0
hsa-miR-220 0.0 0.0 0.0 0.0 0.0 0.0 0.0
hsa-miR-221 4475.8 4872.9 1830.1 5095.0 975.3 15978.7 12052.3
hsa-miR-222 15962.5 17110.8 6970.3 35996.9 2972.8 41450.2 35669.2
hsa-miR-223 27882.7 83327.5 36094.5 55742.6 28922.8 161905.5 65951.0
mmu-miR-224 13.4 53.7 13.5 20.6 50.6 422.0 76.0
hsa-miR-296 230.0 406.2 789.9 661.4 211.9 336.7 496.1
hsa-miR-299 0.0 0.0 0.0 0.0 0.0 8.2 3.6
hsa-miR-301 443.8 270.2 94.2 216.0 276.2 624.7 281.5
hsa-miR-302a 0.0 0.0 17.6 0.0 0.0 0.0 0.0
hsa-miR-302a* 0.0 0.0 0.0 0.0 0.0 0.0 0.0
hsa-miR-302b 545.0 808.2 1018.0 959.5 460.7 701.3 759.8
Continued
310
Table C.4. continued
hsa-miR-302c 0.0 0.0 0.0 0.0 0.0 0.0 10.0
hsa-miR-302c* 0.0 0.0 0.0 0.0 0.0 0.0 0.0
hsa-miR-302d 3.8 9.1 11.1 3.1 7.1 10.6 15.2
hsa-miR-320 7752.2 15311.9 6563.2 18948.2 4799.1 40129.9 15806.6
hsa-miR-321 31941.4 67090.6 53878.2 14942.5 13477.7 57550.3 60924.3
hsa-miR-323 0.0 2.8 2.5 36.3 10.2 387.2 22.0
hsa-miR-324-3p 2956.4 3860.1 2558.7 3734.3 530.8 2493.2 5522.6
hsa-miR-324-5p 2372.4 1886.1 1014.0 1215.6 375.7 2468.5 2597.0
hsa-miR-325 5.3 6.2 3.1 2.4 15.8 2.1 4.2
hsa-miR-326 4.9 14.9 7.6 9.2 2.9 6.7 23.0
hsa-miR-328 5976.3 4604.5 6492.9 11300.5 1722.7 1595.9 4192.2
hsa-miR-330 61.1 75.6 21.9 43.1 36.7 126.2 107.4
hsa-miR-331 12206.4 9100.6 18822.6 10784.7 3495.0 10122.7 9716.4
hsa-miR-335 1374.9 790.5 1248.8 2915.5 1794.1 15039.1 1882.7
hsa-miR-337 0.0 0.0 15.6 0.0 0.0 10.9 1.8
hsa-miR-338 12.1 43.8 2.7 12.3 32.9 0.0 0.0
hsa-miR-339 118.5 114.8 60.3 243.3 87.4 109.5 156.8
hsa-miR-340 301.9 358.9 464.4 403.0 135.5 323.3 226.7
hsa-miR-342 5755.4 7442.7 4682.1 10006.2 1623.6 4974.7 8559.6
hsa-miR-345 2649.5 1851.5 3362.1 3539.3 503.6 792.1 2745.3
hsa-miR-346 13.1 26.4 26.5 41.3 9.6 13.9 16.0
hsa-miR-361 1397.2 1506.8 1679.6 2573.1 427.6 1437.2 2023.6
hsa-miR-367 0.0 0.0 14.5 0.0 0.0 0.0 0.0
hsa-miR-368 13.9 0.0 0.0 0.0 0.0 9.6 0.0
hsa-miR-369 0.0 3.8 3.7 0.0 0.0 56.5 8.5
hsa-miR-370 225.2 420.5 387.3 1183.1 210.7 2499.9 762.3
hsa-miR-371 0.0 0.0 2.8 8.0 3.1 769.2 1.5
hsa-miR-372 10.5 2.3 20.0 37.5 0.0 2795.4 7.9
hsa-miR-373 12.9 0.0 0.0 2.2 0.0 246.2 0.0
hsa-miR-374 13420.1 13060.8 13121.2 16767.3 4290.1 14582.2 14597.9
hsa-miR-375 305.0 4180.2 46.6 1342.3 219706.0 384.2 19148.5
hsa-miR-376a 197.2 230.1 280.3 1039.9 226.4 5411.4 868.2
hsa-miR-377 2.6 0.0 0.0 0.0 0.0 10.5 0.0
hsa-miR-378 432.7 980.3 7679.2 276.6 89.1 194.8 442.2
hsa-miR-379 67.3 184.5 206.0 944.6 131.5 1511.9 365.3
hsa-miR-380-3p 0.0 0.0 0.0 0.0 0.0 3.2 0.0
hsa-miR-380-5p 0.0 12.2 0.0 0.0 0.0 64.4 40.4
hsa-miR-381 16.1 15.4 12.8 46.4 11.9 51.8 64.9
hsa-miR-382 163.5 291.5 236.5 1555.9 397.8 4824.9 829.5
hsa-miR-383 3.4 38.3 56.7 2147.4 28.3 7.8 454.8
hsa-miR-384 0.0 0.0 0.0 0.0 0.0 0.0 0.0
hsa-miR-422b 352.7 496.4 2022.1 263.8 0.0 183.1 464.4
hsa-miR-423 1400.0 1245.5 1513.1 1914.2 562.9 918.5 1224.5
mmu-miR-424 0.0 0.0 0.0 0.0 0.0 0.0 0.0
hsa-miR-425 635.4 667.5 335.4 1157.6 0.0 1096.4 1270.6
Continued
311
Table C.4. continued
miRNA Spleen Small
Intestine Trachea Thymus Thyroid Testes
Normal B cell
hsa-let-7a 7188.0 4012.1 6029.4 5546.6 10310.4 8138.0 707.8
hsa-let-7b 29589.8 16506.7 26143.2 23383.8 27150.6 40081.9 2611.6
hsa-let-7c 1544.5 1785.7 8367.4 8127.5 12071.4 17833.5 84.3
hsa-let-7d 717.8 614.8 552.2 727.0 1532.3 685.4 227.8
hsa-let-7e 338.6 185.8 220.0 210.4 325.8 503.3 3.3
mmu-let-7f 0.0 0.0 0.0 0.0 0.0 0.0 0.0
hsa-let-7g 15392.2 10093.1 11957.5 15582.5 18600.4 272.7 113.2
hsa-let-7i 1758.4 924.0 1304.5 1401.5 6719.3 996.6 281.8
hsa-miR-001 802.7 6667.4 4256.5 604.4 55121.3 190.0 0.0
hsa-miR-007 243.6 1766.9 226.7 443.0 12161.6 30.8 0.0
hsa-miR-009 151.4 1744.0 667.8 290.8 206.8 1477.7 25.5
hsa-miR-009* 57.8 367.5 128.7 58.8 93.0 437.8 0.0
hsa-miR-010a 5052.3 5795.6 2596.3 962.7 1277.9 2002.1 16.0
hsa-miR-010b 2819.1 889.3 966.0 1072.1 808.7 5993.6 0.0
hsa-miR-015a 2003.3 1259.6 1495.3 3869.9 1295.6 194.0 64.7
hsa-miR-015b 17069.3 8944.3 6324.8 29926.0 8386.4 15926.4 5266.7
hsa-miR-016 35112.5 11330.4 17248.6 51925.3 27178.6 8.1 6.3
hsa-miR-017-3p 134.4 49.8 65.4 147.1 109.7 19.1 4.1
hsa-miR-017-5p 1643.0 1641.7 1107.6 6085.3 909.7 423.3 60.9
hsa-miR-018 142.5 148.8 77.0 361.3 51.0 171.8 54.0
hsa-miR-019a 11025.7 12603.2 6000.6 20954.7 5815.0 12254.9 1590.5
hsa-miR-019b 59523.0 65503.5 40849.2 109027.2 38453.6 75554.6 12453.0
hsa-miR-020 53992.0 53027.9 36868.9 113277.0 42535.0 61647.5 12383.0
hsa-miR-021 33698.3 36337.4 30273.4 15321.4 19286.2 16718.0 951.4
hsa-miR-022 1344.1 1336.1 1148.1 548.0 1274.8 1458.3 12.6
hsa-miR-023a 329.1 193.9 397.0 216.7 338.1 11.3 0.0
hsa-miR-023b 2001.0 2147.4 2506.5 905.1 2398.2 2442.6 23.0
hsa-miR-023bN 10430.2 15618.8 15565.6 4528.1 11160.2 19383.9 255.0
hsa-miR-024 135165.6 113191.7 149177.4 57463.7 140186.3 126345.7 5594.1
hsa-miR-025 3283.9 2823.5 2001.6 8960.0 2232.6 2860.6 1394.8
hsa-miR-026aN 91386.4 53577.6 94348.5 92098.5 116772.8 95968.8 13895.1
hsa-miR-026b 42712.6 28077.1 32056.6 26702.4 46444.2 31198.9 4599.0
hsa-miR-026bN 35342.5 21028.5 25032.8 21607.4 35599.8 21765.8 4788.9
hsa-miR-027a 14311.5 13071.9 13803.0 6635.1 11155.8 15002.0 332.2
hsa-miR-027b 2035.7 2786.8 3603.9 879.5 2855.4 4397.4 16.6
hsa-miR-027bN 28056.9 32865.5 47071.3 14822.8 57955.7 37981.5 320.9
hsa-miR-028 3673.5 6089.0 3615.0 2916.1 2028.8 3071.1 948.6
hsa-miR-029a 18027.7 15160.2 16732.3 13944.0 27718.2 19602.0 6143.3
hsa-miR-029b 421.5 308.6 346.1 199.6 461.2 412.0 18.2
hsa-miR-029c 12558.2 5621.1 6628.4 7423.8 10471.9 5415.1 3734.3
hsa-miR-030a-3p 4403.2 5209.2 6913.0 2683.5 9835.1 7825.5 65.6
hsa-miR-030c 42349.1 39768.1 40682.7 42625.7 65687.9 47202.7 12270.8
hsa-miR-030d 6646.3 5914.5 6200.8 5862.9 10111.4 5147.6 1624.4
hsa-miR-030e-3p 2521.8 2166.9 2369.9 2983.6 1955.5 4032.3 780.9
hsa-miR-031 446.7 51594.6 13128.3 2934.0 7032.7 8456.3 47.5
Continued
312
Table C.4. continued
hsa-miR-032 2132.0 1688.4 1025.0 2375.5 1514.2 1657.4 113.8
hsa-miR-033 1.9 0.0 2.5 17.5 0.0 67.8 4.6
hsa-miR-034a 102.7 120.2 249.6 203.5 230.2 500.8 3.7
hsa-miR-034aN 1435.5 1480.6 2334.7 2293.3 2557.4 5580.1 14.9
hsa-miR-034b 0.0 9.8 1360.3 36.7 0.0 967.2 0.0
hsa-miR-034b 8.3 0.0 1229.8 59.2 0.0 870.1 0.0
hsa-miR-092N 25844.3 17971.4 17433.3 49898.4 15906.2 26066.3 19970.3
hsa-miR-093 14986.0 16394.2 10689.2 35764.6 11547.8 10777.9 2854.1
hsa-miR-095 8834.0 846.4 921.3 997.9 5282.3 750.4 116.5
hsa-miR-096 582403.0 696132.5 573402.1 396347.7 837447.8 367914.0 471842.4
hsa-miR-098 479.0 242.9 327.2 393.5 670.1 446.4 31.4
hsa-miR-099a 2407.8 5332.0 18839.4 24320.9 27472.4 438.5 0.0
hsa-miR-099b 3347.5 2103.5 2669.4 1868.4 3480.2 4864.6 31.3
hsa-miR-100 2104.1 4611.9 10789.2 16583.7 16845.5 17508.3 0.0
hsa-miR-101 293.4 209.6 181.4 303.0 233.8 246.1 49.9
hsa-miR-103 4569.3 2530.3 2331.1 2785.6 2637.6 3257.8 549.2
hsa-miR-105 803.3 601.3 535.2 1534.7 454.2 870.0 567.4
hsa-miR-106a 54220.8 84168.8 64727.1 46545.4 109061.8 30324.5 52471.3
hsa-miR-106b 4713.2 4221.2 2809.5 9199.3 2872.2 296.9 76.2
hsa-miR-107 4.8 30.5 5.3 16.5 45.9 14.1 0.0
hsa-miR-108 0.0 0.0 0.0 0.0 0.0 0.0 0.0
hsa-miR-122a 0.0 0.0 0.0 0.0 0.0 3.4 0.0
hsa-miR-124a 0.0 0.0 0.0 0.0 0.0 0.0 0.0
hsa-miR-125a 41162.0 27795.2 36905.6 28228.3 48935.3 67233.4 421.7
hsa-miR-125b 6653.0 12497.4 36477.7 44440.7 56981.0 73863.3 17.5
hsa-miR-126 306194.7 88518.2 131469.8 94212.0 201081.6 98601.5 1735.0
hsa-miR-126* 38173.2 11128.4 14712.6 11552.4 18110.1 12545.3 203.3
hsa-miR-127 4796.2 2027.7 3171.5 2861.9 796.8 16842.7 18.0
hsa-miR-128a 0.0 0.0 0.0 7.7 0.0 0.0 0.0
mmu-miR-129-2 0.0 0.0 0.0 0.0 0.0 14.9 0.0
hsa-miR-130a 1917.0 1101.0 790.0 1908.7 2000.5 1752.8 26.9
hsa-miR-130aN 6911.0 4431.9 4738.3 9862.4 15310.3 7786.1 107.8
hsa-miR-130b 68.5 134.5 41.6 284.9 97.6 44.9 2.6
hsa-miR-132 4483.9 4945.8 4855.4 7259.9 2621.9 7860.5 131.0
hsa-miR-133a 80.5 1198.4 813.4 130.0 9296.2 921.1 4.6
hsa-miR-134 539.0 184.7 338.1 198.4 77.8 2017.9 4.0
hsa-miR-135a 56.6 118.9 485.6 402.8 49202.8 4748.0 5.6
hsa-miR-135b 767.0 1925.7 4563.4 565.6 20418.0 12368.7 15.8
mmu-miR-137 0.0 146.1 2.5 21.0 20.3 0.0 0.0
hsa-miR-139 1834.1 188.0 101.2 161.3 163.0 163.7 9.7
hsa-miR-140 17959.5 11373.3 204.3 13010.9 9317.8 19716.7 1962.3
hsa-miR-141 30.3 6699.2 3278.0 1627.4 2922.3 528.5 6.7
hsa-miR-142-3p 146286.3 25459.5 29780.7 158461.1 4822.3 7443.5 22691.4
hsa-miR-142-5p 4233.6 1913.4 2503.2 8236.6 632.2 810.8 3165.1
hsa-miR-143 1584.7 6454.6 2963.4 695.2 1085.5 3651.9 0.0
hsa-miR-145 2557.1 7942.2 4413.5 1115.7 1238.0 5101.9 13.9
Continued
313
Table C.4. continued
hsa-miR-146 31506.8 8747.9 34051.8 28862.9 8255.7 7288.2 8308.8
hsa-miR-146b 24546.0 14637.0 31232.0 20135.9 13911.8 15510.6 1115.6
hsa-miR-147 5.5 9.3 1.7 0.0 3.1 0.0 0.0
hsa-miR-148a 2890.7 5330.2 11556.6 6657.1 4848.1 6089.4 35.1
hsa-miR-148b 732.6 702.0 669.9 613.6 1487.7 932.2 55.3
hsa-miR-149 608.3 1041.6 1527.8 9367.8 311.0 3450.1 8.5
hsa-miR-150 185337.1 38465.6 73851.0 212825.0 18399.5 10549.0 427307.7
hsa-miR-152 2905.4 3528.6 6634.1 4173.9 8561.5 7568.6 116.1
hsa-miR-152N 2548.7 2686.0 4606.5 2716.9 6390.5 5576.3 59.3
hsa-miR-153 27.1 47.1 7.9 2.7 10.2 3.8 0.0
hsa-miR-154 0.0 0.0 0.0 0.0 0.0 0.0 0.0
hsa-miR-154* 10.3 0.0 9.2 0.0 0.0 4.2 0.0
hsa-miR-155 9587.2 3444.0 5880.1 17280.7 2354.8 156.7 653.1
hsa-miR-181a 2864.2 679.7 434.6 25794.1 3552.4 10.2 2.8
hsa-miR-181b 6888.1 2880.2 2632.8 30230.0 7682.4 3314.3 977.0
hsa-miR-181c 203.0 69.8 61.8 1060.0 303.4 0.0 0.0
hsa-miR-182 14.9 12.7 4.0 30.4 15.6 18.1 24.8
hsa-miR-183 4.2 84.5 94.0 922.1 228.7 0.0 0.0
hsa-miR-184 0.0 0.0 11.7 85.1 17.0 74.9 0.0
hsa-miR-186 5712.4 5493.7 5090.0 5504.8 3782.9 7323.1 684.5
hsa-miR-187 47.1 623.2 360.9 9.0 24.2 924.7 0.0
hsa-miR-188b 66.9 76.1 66.3 109.0 81.9 82.4 55.8
hsa-miR-190 80.9 81.4 45.7 42.3 106.5 74.7 0.0
hsa-miR-191 18796.1 11890.3 13267.9 21718.1 16439.0 2852.0 1060.2
hsa-miR-192 1999.3 334215.6 1016.8 1976.3 3082.5 2958.3 323.3
hsa-miR-193 406.1 397.8 334.5 403.5 443.8 283.7 97.7
hsa-miR-194 873.4 145110.3 382.5 759.0 1052.3 1117.2 173.4
hsa-miR-195 25739.8 20908.2 24917.6 17580.3 21127.0 4664.0 34.1
hsa-miR-196a 29.2 81.3 54.7 526.5 23.3 1263.9 0.0
hsa-miR-196b 0.0 0.0 0.0 462.3 0.0 4.3 0.0
hsa-miR-197 6054.1 3795.1 3340.2 5434.1 2102.4 3514.2 1348.1
hsa-miR-199a 109.7 59.2 60.9 51.6 47.0 242.9 0.0
hsa-miR-199a* 8462.3 5443.0 10428.7 4698.0 5608.8 1111.8 0.0
hsa-miR-199b 9.0 43.9 73.0 60.2 61.3 4.4 0.0
hsa-miR-199-s 656.1 429.8 799.8 334.3 399.7 250.9 0.0
hsa-miR-200a 40.8 19355.7 18843.7 9651.6 7837.4 371.5 2.0
hsa-miR-200b 25.4 15116.7 23077.8 5989.6 5223.9 1139.1 13.6
hsa-miR-200bN 93.3 19970.8 27034.9 13968.8 11593.9 103.1 3.2
hsa-miR-200c 388.2 85635.8 87730.1 18637.4 78343.7 7330.2 503.1
hsa-miR-200cN 277.4 54586.0 57431.8 10550.2 53186.4 556.0 37.1
hsa-miR-203 37.0 1843.8 2203.8 6554.3 5302.9 115.7 0.0
hsa-miR-204 4203.4 419.8 1698.9 2377.3 3387.7 26781.3 0.0
hsa-miR-205 0.0 0.0 6503.6 20956.2 64.5 654.9 0.0
hsa-miR-206 0.0 6.1 900.7 1740.0 14906.2 340.1 0.0
hsa-miR-208 0.0 0.0 0.0 0.0 0.0 0.0 0.0
hsa-miR-210 96.3 628.7 292.1 805.6 211.4 19.0 1.7
Continued
314
Table C.4. continued
hsa-miR-210N 372.0 2714.2 1394.3 1963.6 1000.4 433.2 33.2
hsa-miR-211 5.8 0.0 11.0 165.4 0.0 0.0 0.0
hsa-miR-212 1037.6 961.9 1154.0 1281.4 446.8 1135.8 301.9
hsa-miR-213 43.9 7.7 5.2 568.4 37.1 3.2 0.0
hsa-miR-214 13523.0 9057.7 12764.9 7316.9 10088.8 7671.3 0.0
hsa-miR-215 7.9 81909.2 8.2 12.1 3.7 122.3 0.0
hsa-miR-216 0.0 0.0 0.0 0.0 0.0 0.0 0.0
hsa-miR-217 0.0 1.6 0.0 0.0 0.0 3.0 0.0
hsa-miR-218 9912.9 3506.1 6353.6 3903.7 4072.5 3232.2 20.5
hsa-miR-219 0.0 0.0 0.0 0.0 0.0 0.0 0.0
hsa-miR-220 0.0 0.0 0.0 0.0 0.0 0.0 0.0
hsa-miR-221 1540.5 2277.8 2233.2 2185.1 1679.7 2093.5 254.0
hsa-miR-222 5126.6 8085.8 9507.8 8978.5 7707.1 6393.4 1345.5
hsa-miR-223 552278.1 36160.2 136543.5 56461.9 40258.8 24194.0 59716.9
mmu-miR-224 11.2 44.0 21.6 39.5 44.1 21.9 0.0
hsa-miR-296 272.8 176.6 376.7 544.0 505.2 544.0 102.5
hsa-miR-299 0.0 0.0 0.0 0.0 0.0 10.1 0.0
hsa-miR-301 250.8 218.1 121.6 454.8 112.7 309.3 34.4
hsa-miR-302a 0.0 0.0 0.0 0.0 0.0 0.0 0.0
hsa-miR-302a* 0.0 0.0 0.0 0.0 0.0 0.0 0.0
hsa-miR-302b 2120.8 530.9 1635.4 1051.2 1298.5 721.9 727.2
hsa-miR-302c 29.2 0.0 21.3 0.0 18.8 0.0 17.3
hsa-miR-302c* 0.0 0.0 0.0 0.0 0.0 0.0 0.0
hsa-miR-302d 5.6 8.6 11.7 0.0 7.0 0.0 0.0
hsa-miR-320 7978.0 8170.9 9180.7 7904.8 7391.1 7702.4 3927.5
hsa-miR-321 26118.3 46330.9 38108.7 31798.8 27618.0 29289.2 4207.4
hsa-miR-323 15.3 5.1 0.0 5.4 0.0 146.9 0.0
hsa-miR-324-3p 2292.7 2218.2 2019.2 2665.2 3361.8 2869.6 402.0
hsa-miR-324-5p 948.8 695.9 614.3 787.0 965.5 803.9 17.3
hsa-miR-325 11.3 16.3 5.7 1.4 7.0 3.9 16.1
hsa-miR-326 22.5 30.0 6.2 18.7 4.4 9.7 0.0
hsa-miR-328 2818.1 1591.3 2808.0 3789.6 3425.7 5468.0 574.9
hsa-miR-330 68.1 48.1 47.1 73.5 43.8 37.9 21.6
hsa-miR-331 5606.3 4158.5 5257.8 5986.9 8418.7 6567.2 1511.1
hsa-miR-335 3121.3 1186.7 1749.5 1489.8 1427.7 2173.8 48.3
hsa-miR-337 0.0 3.5 0.0 0.0 0.0 2.2 0.0
hsa-miR-338 17.2 33.8 4.4 14.9 4.3 0.0 0.0
hsa-miR-339 99.5 48.1 81.6 58.4 48.9 84.9 83.4
hsa-miR-340 387.8 211.4 227.8 136.1 202.2 266.7 31.3
hsa-miR-342 14736.1 6954.1 6739.3 28735.9 3235.3 5622.7 9338.8
hsa-miR-345 1717.8 1539.2 2590.8 2503.0 7157.1 2666.1 281.1
hsa-miR-346 4.8 22.6 26.8 24.5 66.2 33.8 4.1
hsa-miR-361 933.3 599.5 714.9 928.0 762.2 1149.3 179.4
hsa-miR-367 0.0 0.0 0.0 0.0 0.0 0.0 0.0
hsa-miR-368 6.9 0.0 0.0 0.0 0.0 4.7 0.0
hsa-miR-369 15.2 2.0 13.4 3.0 3.6 15.1 0.0
Continued
315
Table C.4. continued
hsa-miR-370 1160.8 235.8 435.0 391.2 98.7 1563.8 99.2
hsa-miR-371 13.5 3.5 1.7 9.2 7.5 98.6 0.0
hsa-miR-372 3.8 2.6 6.4 7.7 2.7 487.7 3.8
hsa-miR-373 0.0 0.0 0.0 0.0 0.0 54.0 0.0
hsa-miR-374 13252.1 9719.9 9234.4 8584.8 9448.3 11283.3 1060.6
hsa-miR-375 65.4 28817.5 23824.7 1911.2 775.9 1793.2 0.0
hsa-miR-376a 698.0 168.8 293.7 188.7 111.0 930.3 8.1
hsa-miR-377 0.0 0.0 0.0 0.0 0.0 13.5 0.0
hsa-miR-378 132.8 452.8 233.2 176.6 425.3 78.9 67.2
hsa-miR-379 288.2 111.6 196.7 143.3 53.9 979.2 0.0
hsa-miR-380-3p 0.0 0.0 0.0 0.0 0.0 0.0 0.0
hsa-miR-380-5p 11.2 0.0 0.0 0.0 1.9 49.0 0.0
hsa-miR-381 3.2 15.8 19.5 33.6 1.9 38.3 0.0
hsa-miR-382 1029.7 326.3 439.4 298.5 133.5 1690.1 0.0
hsa-miR-383 9.3 114.8 102.2 1070.1 4.6 947.8 0.0
hsa-miR-384 0.0 0.0 0.0 0.0 0.0 0.0 0.0
hsa-miR-422b 77.1 660.5 141.5 99.6 142.8 59.5 9.3
hsa-miR-423 1292.8 878.4 1008.1 1552.9 1181.7 1304.3 677.1
mmu-miR-424 0.0 0.0 0.0 0.0 0.0 19.1 0.0
hsa-miR-425 607.3 456.4 548.1 446.2 571.9 581.8 127.2
316
Normal tissues Cell lines
mature precursor pearson p-value pearson p-value
hsa-let-7a let-7a-1* -0.101 0.654 0.4 0.014
hsa-let-7a let-7a-2-P 0.334 0.128 -0.125 0.462
hsa-let-7a let-7a-3* 0.465 0.029 0.356 0.031
hsa-let-7b let-7b* 0.735 < 0.0001 0.739 < 0.0001
hsa-let-7c let-7c* 0.069 0.759 0.963 < 0.0001
hsa-let-7d let-7d* 0.213 0.341 0.331 0.046
hsa-let-7e let-7e* 0.683 0.000 0.478 0.003
mmu-let-7f let-7f-1* 0.000 1.000 0 1
mmu-let-7f let-7f-2* 0.000 1.000 0 1
hsa-let-7g let-7g* -0.448 0.037 0.541 0.001
hsa-let-7i let-7i* -0.100 0.656 0.292 0.08
hsa-miR-001 MIR-001-1 0.914 < 0.0001 -0.014 0.936
hsa-miR-001 MIR-001-2 0.933 < 0.0001 -0.041 0.809
hsa-miR-007 MIR-007-1 -0.098 0.664 0.19 0.26
hsa-miR-007 MIR-007-2 0.379 0.082 0.15 0.375
hsa-miR-007 MIR-007-3 0.434 0.044 0.64 < 0.0001
hsa-miR-009 MIR-009-1-P 0.294 0.184 0.081 0.635
hsa-miR-009 MIR-009-2-P 1.000 < 0.0001 -0.008 0.961
hsa-miR-009 MIR-009-3-P 0.992 < 0.0001 0.046 0.785
hsa-miR-009* MIR-009-1-P 0.294 0.184 0.079 0.641
hsa-miR-009* MIR-009-2-P 1.000 < 0.0001 0.077 0.649
hsa-miR-009* MIR-009-3-P 0.992 < 0.0001 0.123 0.47
hsa-miR-010a MIR-010a,b 0.774 < 0.0001 0.574 0
hsa-miR-015a MIR-015a -0.320 0.146 0.403 0.013
hsa-miR-015b MIR-015b 0.492 0.020 0.253 0.13
hsa-miR-016 MIR-016-1 0.112 0.620 0.58 0
hsa-miR-016 MIR-016-2 0.116 0.608 0.297 0.074
hsa-miR-017-3p MIR-017-P -0.220 0.326 0.728 < 0.0001
hsa-miR-017-5p MIR-017-P -0.215 0.336 0.404 0.013
hsa-miR-018 MIR-018 0.257 0.249 0.754 < 0.0001
hsa-miR-019a MIR-019a-P -0.328 0.136 0.808 < 0.0001
hsa-miR-019b MIR-019b-1-P -0.325 0.140 0.219 0.193
hsa-miR-019b MIR-019b-2-P -0.097 0.667 0.131 0.44
hsa-miR-020 MIR-020 -0.393 0.071 0.394 0.016
hsa-miR-021 MIR-021 0.452 0.035 0.522 0.001
hsa-miR-022 MIR-022-P -0.286 0.197 -0.13 0.444
hsa-miR-023a MIR-023a 0.403 0.063 0.151 0.372
hsa-miR-023b MIR-023b-P 0.395 0.069 0.256 0.126
hsa-miR-023bN MIR-023b-P 0.244 0.275 0.213 0.206
hsa-miR-024 MIR-024-1,2 0.644 0.001 0.445 0.006
hsa-miR-025 MIR-025 0.189 0.399 0.479 0.003
hsa-miR-026aN MIR-026a-1,2 0.182 0.416 0.373 0.023
Continued
Table C.5. Correlation between mature and precursor miRNA expression in
Normal tissues and Cell lines.
317
Table C.5. continued
hsa-miR-026b MIR-026b -0.104 0.644 0.116 0.495
hsa-miR-026bN MIR-026b -0.071 0.753 0.175 0.299
hsa-miR-027a MIR-027a, b 0.328 0.136 0.07 0.68
hsa-miR-027b MIR-027a, b 0.058 0.797 0.159 0.348
hsa-miR-027bN MIR-027a, b 0.102 0.653 0.111 0.512
hsa-miR-028 MIR-028 0.170 0.450 0.16 0.345
hsa-miR-029a MIR-029a,c -0.127 0.575 0.774 < 0.0001
hsa-miR-029b MIR-029b-1,2 0.000 1.000 0.024 0.887
hsa-miR-029c MIR-029a,c -0.333 0.130 0.178 0.291
hsa-miR-030a-3p MIR-030a,e 0.435 0.043 0.547 0
hsa-miR-030c MIR-030c-1,2 -0.074 0.744 0.181 0.282
hsa-miR-030d MIR-030d 0.777 < 0.0001 0.736 < 0.0001
hsa-miR-030e-3p MIR-030a,e -0.185 0.411 0.515 0.001
hsa-miR-031 MIR-031-P 0.220 0.325 0.173 0.306
hsa-miR-032 MIR-032 -0.008 0.973 0.413 0.011
hsa-miR-033 MIR-033 -0.088 0.697 0.419 0.01
hsa-miR-034a MIR-034a 0.646 0.001 -0.113 0.506
hsa-miR-034aN MIR-034a 0.632 0.002 -0.046 0.788
hsa-miR-034b MIR-034b 0.268 0.228 0.176 0.298
hsa-miR-092N MIR-092-1 -0.134 0.552 0.757 < 0.0001
hsa-miR-092N MIR-092-2-P -0.083 0.713 0.27 0.106
hsa-miR-093 MIR-093 0.222 0.320 0.163 0.336
hsa-miR-095 MIR-095 0.282 0.204 0.33 0.046
hsa-miR-096 MIR-096-P 0.219 0.328 0.291 0.081
hsa-miR-098 MIR-098 0.273 0.219 0.357 0.03
hsa-miR-099a MIR-099a 0.081 0.719 0.258 0.123
hsa-miR-099b MIR-099b 0.721 0.000 0.071 0.675
hsa-miR-100 MIR-100 0.742 < 0.0001 0.52 0.001
hsa-miR-101 MIR-101-1 -0.225 0.315 0.342 0.038
hsa-miR-101 MIR-101-2 0.263 0.236 0.443 0.006
hsa-miR-103 MIR-103-1,2 0.179 0.424 0.136 0.421
hsa-miR-105 MIR-105-1,2 0.140 0.534 0.08 0.638
hsa-miR-106a MIR-106a-P -0.035 0.877 0.416 0.01
hsa-miR-106b MIR-106b 0.487 0.022 0.415 0.011
hsa-miR-107 MIR-107 0.207 0.355 -0.001 0.997
hsa-miR-108 MIR-108 0.000 1.000 0 1
hsa-miR-122a MIR-122a 0.178 0.429 0 1
hsa-miR-124a MIR-124a-1,2,3 1.000 < 0.0001 0 1
hsa-miR-125a MIR-125a 0.884 < 0.0001 0.717 < 0.0001
hsa-miR-125b MIR-125b-1 0.909 < 0.0001 0.395 0.016
hsa-miR-125b MIR-125b-2 0.889 < 0.0001 0.235 0.162
hsa-miR-126 MIR-126 0.470 0.027 0.835 < 0.0001
hsa-miR-126* MIR-126 0.315 0.153 0.806 < 0.0001
hsa-miR-127 MIR-127 0.298 0.177 0.121 0.476
hsa-miR-128a MIR-128a 0.720 0.000 0 1
mmu-miR-129-2 MIR-129-2 0.565 0.006 0.098 0.565
Continued
318
Table C.5. continued
hsa-miR-130a MIR-130a-P -0.191 0.395 0.34 0.039
hsa-miR-130aN MIR-130a-P -0.165 0.464 0.294 0.078
hsa-miR-130b MIR-130b -0.152 0.500 0.613 < 0.0001
hsa-miR-132 MIR-132 0.212 0.343 0.274 0.101
hsa-miR-133a MIR-133a-1,2 -0.021 0.926 -0.086 0.612
hsa-miR-134 MIR-134 0.563 0.006 0.082 0.631
hsa-miR-135a MIR-135a-1,2 -0.051 0.823 0.028 0.867
hsa-miR-135b MIR-135b-P -0.051 0.823 0.227 0.176
mmu-miR-137 MIR-137* 0.991 < 0.0001 0.689 < 0.0001
hsa-miR-139 MIR-139 -0.207 0.356 -0.033 0.847
hsa-miR-140 MIR-140 -0.385 0.077 0.084 0.62
hsa-miR-141 MIR-141 0.467 0.028 0.667 < 0.0001
hsa-miR-142-3p MIR-142-P 0.126 0.578 0.327 0.049
hsa-miR-142-5p MIR-142-P 0.203 0.365 0.278 0.096
hsa-miR-143 MIR-143 0.672 0.001 -0.163 0.335
hsa-miR-145 MIR-145-P 0.327 0.137 0.136 0.422
hsa-miR-146b MIR-146 0.117 0.604 0.09 0.595
hsa-miR-147 MIR-147 0.088 0.696 0 1
hsa-miR-148a MIR-148a-P -0.157 0.485 0.135 0.427
hsa-miR-148b MIR-148b-P 0.053 0.813 0.186 0.269
hsa-miR-149 MIR-149 0.932 < 0.0001 0.159 0.347
hsa-miR-150 MIR-150 0.874 < 0.0001 0.413 0.011
hsa-miR-152 MIR-152 0.293 0.186 0.716 < 0.0001
hsa-miR-152N MIR-152 0.229 0.305 0.712 < 0.0001
hsa-miR-153 MIR-153-1,2 0.785 < 0.0001 0.417 0.01
hsa-miR-154 MIR-154 0.576 0.005 0 1
hsa-miR-154* MIR-154 0.732 0 0 1
hsa-miR-155 MIR-155 0.693 0 0.784 < 0.0001
hsa-miR-181a MIR-181a,c 0.503 0.017 0.099 0.561
hsa-miR-181b MIR-181b-1,2 0.282 0.203 0.717 < 0.0001
hsa-miR-181c MIR-181a,c 0.466 0.029 0.165 0.329
hsa-miR-182 MIR-182 -0.013 0.953 0.239 0.154
hsa-miR-183 MIR-183 0.016 0.943 0.091 0.594
hsa-miR-184 MIR-184 0.131 0.561 -0.065 0.702
hsa-miR-186 MIR-186-P -0.083 0.713 0.418 0.01
hsa-miR-187 MIR-187 -0.147 0.515 -0.016 0.924
hsa-miR-188b MIR-188b-P -0.097 0.668 -0.061 0.72
hsa-miR-190 MIR-190 0 1 0 1
hsa-miR-191 MIR-191 -0.39 0.072 0.208 0.217
hsa-miR-192 MIR-192 -0.125 0.578 -0.158 0.351
hsa-miR-193 MIR-193 0.359 0.1 0.024 0.888
hsa-miR-194 MIR-194-1,2 -0.107 0.636 -0.078 0.647
hsa-miR-195 MIR-195-P 0.002 0.993 0.094 0.58
hsa-miR-196a MIR-196a-1 0.069 0.76 -0.115 0.499
hsa-miR-196a MIR-196a-2 0.594 0.004 0.114 0.503
hsa-miR-196b MIR-196b 0.911 < 0.0001 0.192 0.256
Continued
319
Table C.5. continued
hsa-miR-197 MIR-197 -0.177 0.432 -0.031 0.856
hsa-miR-199a MIR-199a-1-P -0.294 0.184 0 1
hsa-miR-199a MIR-199a-2-P 0.513 0.015 0 1
hsa-miR-199a* MIR-199a-1-P -0.276 0.213 0.052 0.759
hsa-miR-199a* MIR-199a-2-P 0.548 0.008 -0.152 0.368
hsa-miR-199b MIR-199b-P 0.728 0 0.093 0.585
hsa-miR-200b MIR-200b 0.888 < 0.0001 0.823 < 0.0001
hsa-miR-200bN MIR-200b 0.894 < 0.0001 0.822 < 0.0001
hsa-miR-200c MIR-200c 0.412 0.057 0.378 0.021
hsa-miR-200cN MIR-200c 0.426 0.048 0.383 0.019
hsa-miR-203 MIR-203 0.905 < 0.0001 0.342 0.038
hsa-miR-204 MIR-204 0.915 < 0.0001 -0.083 0.625
hsa-miR-205 MIR-205 0.712 0 0.95 < 0.0001
hsa-miR-206 MIR-206 0.998 < 0.0001 0.02 0.908
hsa-miR-208 MIR-208 0.976 < 0.0001 0 1
hsa-miR-210 MIR-210 0.584 0.004 0.205 0.224
hsa-miR-210N MIR-210 0.579 0.005 0.212 0.208
hsa-miR-211 MIR-211 0.204 0.363 -0.048 0.779
hsa-miR-212 MIR-212 0.209 0.352 0.053 0.756
hsa-miR-213 MIR-213 0.782 < 0.0001 0.711 < 0.0001
hsa-miR-214 MIR-214 -0.12 0.595 0.015 0.932
hsa-miR-215 MIR-215 0.064 0.779 -0.051 0.762
hsa-miR-216 MIR-216 0.911 < 0.0001 0 1
hsa-miR-217 MIR-217 0 1 0.147 0.385
hsa-miR-218 MIR-218-1,2 0.705 0 0.083 0.626
hsa-miR-219 MIR-219-1,2 -0.067 0.769 -0.102 0.548
hsa-miR-220 MIR-220 0 1 0 1
hsa-miR-221 MIR-221 0.095 0.674 0.5 0.002
hsa-miR-222 MIR-222-P 0.364 0.096 0.253 0.131
hsa-miR-223 MIR-223 0.427 0.047 0.519 0.001
mmu-miR-224 MIR-224 0.848 < 0.0001 0.462 0.004
hsa-miR-296 MIR-296 0.324 0.141 0.097 0.567
hsa-miR-299 MIR-299-P -0.036 0.874 0 1
hsa-miR-301 MIR-301 0.311 0.158 0.565 0
hsa-miR-302a MIR-302 -0.189 0.4 0.003 0.988
hsa-miR-302a* MIR-302 0 1 0 1
hsa-miR-302b MIR-302b -0.023 0.919 0.322 0.052
hsa-miR-302c MIR-302c -0.103 0.65 -0.011 0.948
hsa-miR-302c* MIR-302c 0 1 0 1
hsa-miR-302d MIR-302d -0.109 0.629 0.101 0.553
hsa-miR-320 MIR-320 0.3 0.175 0.239 0.154
hsa-miR-321 MIR-321 0.538 0.01 0.455 0.005
hsa-miR-323 MIR-323 0.663 0.001 0.343 0.038
hsa-miR-324-3p MIR-324-P -0.239 0.285 0.081 0.633
hsa-miR-324-5p MIR-324-P -0.203 0.365 0.02 0.907
hsa-miR-325 MIR-325 -0.044 0.846 -0.104 0.542
Continued
320
Table C.5. continued
hsa-miR-326 MIR-326-P 0.608 0.003 -0.161 0.341
hsa-miR-328 MIR-328 -0.073 0.747 0.142 0.401
hsa-miR-330 MIR-330 0.852 < 0.0001 0.189 0.262
hsa-miR-331 MIR-331-P 0.177 0.43 0.349 0.035
hsa-miR-335 MIR-335 -0.151 0.503 -0.127 0.453
hsa-miR-337 MIR-337 0.323 0.142 0 1
hsa-miR-338 MIR-338 0.919 < 0.0001 -0.136 0.421
hsa-miR-339 MIR-339 0.413 0.056 -0.068 0.69
hsa-miR-340 MIR-340 0.839 < 0.0001 0.591 0
hsa-miR-342 MIR-342 0.499 0.018 0.027 0.875
hsa-miR-345 MIR-345 -0.092 0.682 0.097 0.568
hsa-miR-346 MIR-346 0.279 0.208 0.057 0.738
hsa-miR-361 MIR-361 -0.037 0.87 -0.014 0.935
hsa-miR-367 MIR-367 -0.213 0.341 0.089 0.599
hsa-miR-368 MIR-368 0.425 0.049 0 1
hsa-miR-369 MIR-369 0.809 < 0.0001 0 1
hsa-miR-370 MIR-370 0.722 0 0.313 0.059
hsa-miR-371 MIR-371 -0.093 0.679 -0.027 0.873
hsa-miR-372 MIR-372 0 1 -0.005 0.978
hsa-miR-373 MIR-373 -0.092 0.684 0.059 0.73
hsa-miR-374 MIR-374-P -0.445 0.038 -0.263 0.116
hsa-miR-375 MIR-375 0.617 0.002 0.508 0.001
hsa-miR-376a MIR-376 0.945 < 0.0001 0.974 < 0.0001
hsa-miR-377 MIR-377 0.518 0.014 0.064 0.709
hsa-miR-378 MIR-378 0.772 < 0.0001 0.278 0.096
hsa-miR-379 MIR-379 0.87 < 0.0001 0.741 < 0.0001
hsa-miR-380-3p MIR-380 0.931 < 0.0001 0 1
hsa-miR-380-5p MIR-380 0.674 0.001 0.285 0.087
hsa-miR-381 MIR-381 0.264 0.236 0.116 0.495
hsa-miR-382 MIR-382 0.734 0 0.107 0.53
hsa-miR-383 MIR-383 0.862 < 0.0001 0.34 0.04
hsa-miR-384 MIR-384 1 < 0.0001 0 1
hsa-miR-422b MIR-422b -0.179 0.424 0.161 0.34
hsa-miR-423 MIR-423 0.05 0.826 0.412 0.011
mmu-miR-424 MIR-424 0.431 0.045 0.000 1
hsa-miR-425 MIR-425 -0.338 0.124 0.006 0.972
hsa-miR-010b MIR-010a,b 0.334 0.043
hsa-miR-030b MIR-030b 0 1
hsa-miR-138 MIR-138-1,2 0.104 0.54
hsa-miR-151 MIR-151 0 1
hsa-miR-200a MIR-200a-P 0.286 0.086
321
Pancreatic tissues Liver tissues
mature precursor pearson p-value pearson p-value
hsa-let-7a let-7a-1* 0.377 0.15 -0.18250 0.24936
hsa-let-7a let-7a-2-P 0.925 < 0.0001 -0.25177 0.17344
hsa-let-7a let-7a-3* 0.696 0.003 -0.05620 0.41812
hsa-let-7b let-7b* 0.772 0 0.05478 0.42015
hsa-let-7c let-7c* 0.623 0.01 0.00000 0.50000
hsa-let-7d let-7d* 0.859 < 0.0001 0.51078 0.02160
hsa-let-7e let-7e* 0.773 0 0.86061 < 0.0001
hsa-let-7f let-7f-1* 0.909 < 0.0001 0.36040 0.08515
hsa-let-7f let-7f-2* 0.648 0.007 0.00000 0.50000
hsa-let-7g let-7g* 0.854 < 0.0001 0.18968 0.24084
hsa-let-7i let-7i* 0.647 0.007 0.27647 0.14997
hsa-miR-001 MIR-001-1 0.044 0.871 -0.38425 0.07086
hsa-miR-001 MIR-001-2 0.607 0.013 0.41642 0.05431
hsa-miR-007 MIR-007-1 -0.122 0.654 0.34160 0.09767
hsa-miR-007 MIR-007-2 0.383 0.143 -0.20180 0.22678
hsa-miR-007 MIR-007-3 0.904 < 0.0001 0.03250 0.45245
hsa-miR-009 MIR-009-1-P -0.322 0.224 -0.28065 0.14620
hsa-miR-009 MIR-009-2-P 0.129 0.633 -0.26464 0.16097
hsa-miR-009 MIR-009-3-P -0.025 0.927 -0.25489 0.17037
hsa-miR-009* MIR-009-1-P -0.444 0.085 -0.29059 0.13745
hsa-miR-009* MIR-009-2-P 0.076 0.78 -0.27327 0.15290
hsa-miR-009* MIR-009-3-P -0.3 0.26 -0.26322 0.16232
hsa-miR-010a MIR-010a,b 0.897 < 0.0001 0.04191 0.43876
hsa-miR-010b MIR-010a,b 0.774 0 0.28917 0.13868
hsa-miR-015a MIR-015a 0.871 < 0.0001 -0.05809 0.41539
hsa-miR-015b MIR-015b 0.868 < 0.0001 0.64628 0.00342
hsa-miR-016 MIR-016-1 0.829 < 0.0001 0.71353 0.00096
hsa-miR-016 MIR-016-2 -0.582 0.018 -0.15797 0.27950
hsa-miR-017-3p MIR-017-P -0.255 0.341 -0.23060 0.19510
hsa-miR-017-5p MIR-017-P -0.391 0.134 -0.27363 0.15257
hsa-miR-018 MIR-018 0.879 < 0.0001 0.84429 < 0.0001
hsa-miR-019a MIR-019a-P 0.061 0.823 0.87615 < 0.0001
hsa-miR-019b MIR-019b-1-P -0.54 0.031 -0.37460 0.07643
hsa-miR-019b MIR-019b-2-P -0.342 0.195 -0.26528 0.16035
hsa-miR-020 MIR-020 0.213 0.429 -0.13270 0.31209
hsa-miR-021 MIR-021 0.94 < 0.0001 0.84599 < 0.0001
hsa-miR-022 MIR-022-P 0.477 0.062 -0.05671 0.41738
hsa-miR-023a MIR-023a 0.959 < 0.0001 -0.41451 0.05521
hsa-miR-023b MIR-023b-P 0.688 0.003 -0.01457 0.47864
hsa-miR-023bN MIR-023b-P 0.631 0.009 0.11443 0.33652
hsa-miR-024 MIR-024-1,2 0.972 < 0.0001 0.68072 0.00185
hsa-miR-025 MIR-025 0.533 0.033 -0.32747 0.10784
Continued
Table C.6. Correlation between mature and precursor miRNA expression in
pancreas tissues and liver tissues.
322
Table C.6. continued
hsa-miR-026aN MIR-026a-1,2 0.747 0.001 -0.10979 0.34282
hsa-miR-026b MIR-026b -0.698 0.003 -0.20663 0.22130
hsa-miR-026bN MIR-026b -0.693 0.003 -0.19477 0.23489
hsa-miR-027a MIR-027a, b 0.943 < 0.0001 -0.50024 0.02423
hsa-miR-027b MIR-027a, b 0.886 < 0.0001 -0.23204 0.19358
hsa-miR-027bN MIR-027a, b 0.845 < 0.0001 -0.27257 0.15355
hsa-miR-028 MIR-028 0.683 0.004 0.57878 0.00941
hsa-miR-029a MIR-029a,c 0.876 < 0.0001 0.37490 0.07625
hsa-miR-029b MIR-029b-1,2 -0.279 0.295 -0.06469 0.40593
hsa-miR-029c MIR-029a,c 0.643 0.007 0.12518 0.32207
hsa-miR-030a-3p MIR-030a,e 0.224 0.405 -0.51751 0.02003
hsa-miR-030c MIR-030c-1,2 0.567 0.022 -0.28793 0.13976
hsa-miR-030d MIR-030d 0.491 0.053 0.31596 0.11660
hsa-miR-030e-3p MIR-030a,e 0.354 0.178 -0.18962 0.24092
hsa-miR-031 MIR-031-P -0.412 0.113 -0.08562 0.37628
hsa-miR-032 MIR-032 0.451 0.079 0.66974 0.00227
hsa-miR-033 MIR-033 0.183 0.498 -0.02136 0.46872
hsa-miR-034a MIR-034a 0.755 0.001 0.13836 0.30467
hsa-miR-034aN MIR-034a 0.727 0.001 -0.14061 0.30175
hsa-miR-034b MIR-034b -0.588 0.017 -0.12820 0.31805
hsa-miR-034c MIR-034c 0 1 0.00000 0.50000
hsa-miR-034cN MIR-034c 0 1 0.00000 0.50000
hsa-miR-092N MIR-092-1 0.233 0.384 -0.21403 0.21303
hsa-miR-092N MIR-092-2-P -0.39 0.135 -0.14858 0.29144
hsa-miR-093 MIR-093 0.446 0.083 -0.20052 0.22825
hsa-miR-095 MIR-095 0.404 0.12 -0.48102 0.02963
hsa-miR-096 MIR-096-P 0.298 0.261 0.24300 0.18225
hsa-miR-098 MIR-098 0.854 < 0.0001 0.51564 0.02046
hsa-miR-099a MIR-099a 0.585 0.017 -0.26063 0.16480
hsa-miR-099b MIR-099b 0.386 0.14 -0.53726 0.01593
hsa-miR-100 MIR-100 0.941 < 0.0001 0.89033 < 0.0001
hsa-miR-101 MIR-101-1 -0.184 0.496 -0.02577 0.46227
hsa-miR-101 MIR-101-2 -0.135 0.619 0.68827 0.00160
hsa-miR-103 MIR-103-1,2 0.914 < 0.0001 0.48609 0.02813
hsa-miR-105 MIR-105-1,2 -0.16 0.554 -0.21435 0.21268
hsa-miR-106a MIR-106a-P 0.239 0.372 0.09714 0.36022
hsa-miR-106b MIR-106b 0.9 < 0.0001 0.79738 0.00011
hsa-miR-107 MIR-107 0.547 0.028 0.73696 0.00056
hsa-miR-108 MIR-108 -0.071 0.793 0.00000 0.50000
hsa-miR-122a MIR-122a -0.217 0.42 0.50642 0.02266
hsa-miR-124a MIR-124a-1,2,3 0 1 0.00000 0.50000
hsa-miR-125a MIR-125a 0.595 0.015 -0.26899 0.15687
hsa-miR-125b MIR-125b-1 0.799 0 0.35778 0.08682
hsa-miR-125b MIR-125b-2 0.718 0.002 0.00205 0.49699
hsa-miR-126 MIR-126 0.821 < 0.0001 0.78681 0.00015
hsa-miR-126* MIR-126 0.816 0 0.82973 < 0.0001
Continued
323
Table C.6. continued
hsa-miR-127 MIR-127 -0.827 < 0.0001 -0.39102 0.06712
hsa-miR-128a MIR-128a 0 1 0.00000 0.50000
mmu-miR-129-2 MIR-129-2 -0.281 0.292 0.00000 0.50000
hsa-miR-130a MIR-130a-P -0.574 0.02 -0.33159 0.10481
hsa-miR-130aN MIR-130a-P -0.498 0.05 -0.32663 0.10846
hsa-miR-130b MIR-130b 0.13 0.63 -0.14030 0.30214
hsa-miR-132 MIR-132 0.843 < 0.0001 -0.00694 0.48983
hsa-miR-133a MIR-133a-1,2 -0.217 0.419 -0.41368 0.05560
hsa-miR-134 MIR-134 -0.292 0.272 -0.19614 0.23329
hsa-miR-135a MIR-135a-1,2 0.755 0.001 -0.21921 0.20734
hsa-miR-135b MIR-135b-P 0.852 < 0.0001 -0.30854 0.12248
mmu-miR-137 MIR-137* 0.371 0.158 0.67248 0.00216
hsa-miR-139 MIR-139 -0.024 0.93 0.57834 0.00947
hsa-miR-140 MIR-140 -0.321 0.225 -0.02641 0.46132
hsa-miR-141 MIR-141 0.43 0.097 0.05419 0.42101
hsa-miR-142-3p MIR-142-P -0.571 0.021 -0.01849 0.47291
hsa-miR-142-5p MIR-142-P -0.567 0.022 -0.16703 0.26819
hsa-miR-143 MIR-143 0.759 0.001 -0.10653 0.34728
hsa-miR-145 MIR-145-P 0.939 < 0.0001 -0.12427 0.32328
hsa-miR-146 MIR-146 -0.167 0.535 -0.10739 0.34610
hsa-miR-146b MIR-146 -0.154 0.57 -0.27217 0.15392
hsa-miR-148a MIR-148a-P 0.087 0.75 -0.13131 0.31393
hsa-miR-148b MIR-148b-P 0.679 0.004 -0.02773 0.45940
hsa-miR-149 MIR-149 -0.492 0.053 0.34013 0.09870
hsa-miR-150 MIR-150 -0.171 0.525 -0.05861 0.41464
hsa-miR-152 MIR-152 0.873 < 0.0001 -0.13685 0.30664
hsa-miR-153 MIR-153-1,2 0.483 0.058 -0.33352 0.10341
hsa-miR-154 MIR-154 -0.073 0.789 0.83900 < 0.0001
hsa-miR-154* MIR-154 0.276 0.3 0.89514 < 0.0001
hsa-miR-155 MIR-155 0.936 < 0.0001 0.79543 0.00012
hsa-miR-181a MIR-181a,c 0.946 < 0.0001 0.72170 0.00080
hsa-miR-181b MIR-181b-1,2 0.311 0.24 -0.13210 0.31289
hsa-miR-181c MIR-181a,c 0.926 < 0.0001 0.66344 0.00254
hsa-miR-183 MIR-183 0.576 0.019 0.33014 0.10586
hsa-miR-184 MIR-184 -0.186 0.491 -0.20374 0.22458
hsa-miR-186 MIR-186-P 0.755 0.001 0.33770 0.10041
hsa-miR-187 MIR-187 -0.128 0.637 0.37749 0.07473
hsa-miR-188 MIR-188b-P -0.008 0.976 0.37749 0.07473
hsa-miR-190 MIR-190 -0.003 0.991 -0.48602 0.02815
hsa-miR-191 MIR-191 -0.508 0.045 -0.10806 0.34519
hsa-miR-192 MIR-192 -0.4 0.125 -0.29203 0.13621
hsa-miR-193 MIR-193 0.029 0.916 0.25668 0.16862
hsa-miR-194 MIR-194-1,2 -0.436 0.091 -0.33974 0.09897
hsa-miR-195 MIR-195-P -0.531 0.034 -0.37283 0.07748
hsa-miR-196a MIR-196a-1 0.214 0.425 0.13614 0.30757
hsa-miR-196b MIR-196b 0.662 0.005 0.68659 0.00165
Continued
324
Table C.6. continued
hsa-miR-197 MIR-197 -0.508 0.044 -0.31801 0.11501
hsa-miR-199a MIR-199a-2-P 0.241 0.368 0.50014 0.02425
hsa-miR-199b MIR-199b-P -0.559 0.024 0.13411 0.31024
hsa-miR-200a MIR-200a-P -0.408 0.117 -0.45630 0.03782
hsa-miR-200b MIR-200b 0.36 0.171 -0.33746 0.10058
hsa-miR-210 MIR-210 -0.24 0.37 0.42991 0.04826
hsa-miR-210N MIR-210 -0.245 0.36 0.44788 0.04095
hsa-miR-211 MIR-211 -0.289 0.277 0.10396 0.35080
hsa-miR-212 MIR-212 0.754 0.001 0.05598 0.41843
hsa-miR-213 MIR-213 0.784 0 -0.02345 0.46566
hsa-miR-214 MIR-214 -0.7 0.003 -0.26505 0.16058
hsa-miR-215 MIR-215 0.088 0.745 0.16477 0.27099
hsa-miR-216 MIR-216 0.176 0.515 0.00000 0.50000
hsa-miR-217 MIR-217 0.653 0.006 -0.19435 0.23538
hsa-miR-218 MIR-218-1,2 0.937 < 0.0001 0.26708 0.15866
hsa-miR-219 MIR-219-1,2 0.543 0.03 0.48877 0.02736
hsa-miR-220 MIR-220 0 1 0.00000 0.50000
hsa-miR-221 MIR-221 0.871 < 0.0001 0.13328 0.31134
hsa-miR-222 MIR-222-P 0.794 0 -0.46476 0.03485
hsa-miR-223 MIR-223 0.54 0.031 -0.23955 0.18578
mmu-miR-224 MIR-224 0.574 0.02 -0.39486 0.06506
hsa-miR-296 MIR-296 -0.406 0.118 -0.40501 0.05983
hsa-miR-299-3p MIR-299-P -0.403 0.122 0.09685 0.36061
hsa-miR-301 MIR-301 0.855 < 0.0001 0.34673 0.09414
hsa-miR-302a MIR-302 0 1 -0.21158 0.21575
hsa-miR-302a* MIR-302 0 1 0.00000 0.50000
hsa-miR-302b MIR-302b 0.207 0.441 0.48377 0.02881
hsa-miR-302c MIR-302c -0.382 0.144 -0.13736 0.30598
hsa-miR-302c* MIR-302c 0 1 0.57278 0.01020
hsa-miR-302d MIR-302d -0.073 0.787 0.00000 0.50000
hsa-miR-320 MIR-320 -0.129 0.635 -0.29220 0.13606
hsa-miR-321 MIR-321 0.624 0.01 0.36584 0.08173
hsa-miR-323 MIR-323 0.281 0.292 0.22682 0.19912
hsa-miR-324-3p MIR-324-P -0.511 0.043 -0.39547 0.06474
hsa-miR-324-5p MIR-324-P -0.253 0.344 -0.49403 0.02589
hsa-miR-325 MIR-325 -0.297 0.264 0.00000 0.50000
hsa-miR-326 MIR-326-P -0.197 0.464 -0.26311 0.16242
hsa-miR-328 MIR-328 -0.423 0.102 -0.46686 0.03414
hsa-miR-330 MIR-330 0.215 0.424 -0.43491 0.04614
hsa-miR-331 MIR-331-P -0.31 0.243 -0.27300 0.15315
hsa-miR-335 MIR-335 -0.16 0.554 -0.18301 0.24875
hsa-miR-337 MIR-337 -0.166 0.539 0.00000 0.50000
hsa-miR-338 MIR-338 -0.472 0.065 -0.30799 0.12292
hsa-miR-339 MIR-339 0.211 0.433 -0.35844 0.08640
hsa-miR-340 MIR-340 0.785 0 0.85806 < 0.0001
hsa-miR-342 MIR-342 0.278 0.297 -0.44967 0.04027
Continued
325
Table C.6. continued
hsa-miR-345 MIR-345 -0.599 0.014 -0.33898 0.09951
hsa-miR-346 MIR-346 0.407 0.117 0.04167 0.43911
hsa-miR-361 MIR-361 -0.588 0.017 -0.36522 0.08212
hsa-miR-367 MIR-367 0.167 0.536 -0.32884 0.10683
hsa-miR-368 MIR-368 -0.278 0.297 0.28113 0.14576
hsa-miR-369 MIR-369 0.364 0.166 0.35733 0.08711
hsa-miR-370 MIR-370 0.304 0.252 0.95425 < 0.0001
hsa-miR-371 MIR-371 0.347 0.188 0.04957 0.42767
hsa-miR-372 MIR-372 -0.388 0.137 -0.23649 0.18894
hsa-miR-373 MIR-373 -0.087 0.749 -0.14928 0.29055
hsa-miR-374 MIR-374-P 0.465 0.07 0.41782 0.05366
hsa-miR-375 MIR-375 0.56 0.024 0.19890 0.23011
hsa-miR-376a MIR-376 0.841 < 0.0001 0.99230 < 0.0001
hsa-miR-377 MIR-377 0.464 0.07 -0.18468 0.24676
hsa-miR-378 MIR-378 -0.488 0.055 0.17028 0.26419
hsa-miR-379 MIR-379 0.792 0 0.99176 < 0.0001
hsa-miR-380-3P MIR-380 -0.055 0.839 0.98244 < 0.0001
hsa-miR-380-5p MIR-380 0.477 0.062 0.43793 0.04489
hsa-miR-381 MIR-381 -0.129 0.634 -0.26214 0.16335
hsa-miR-382 MIR-382 0.096 0.725 -0.00827 0.48788
hsa-miR-383 MIR-383 0.078 0.773 -0.06868 0.40023
hsa-miR-384 MIR-384 0 1 0.00000 0.50000
hsa-miR-422b MIR-422b -0.547 0.028 0.28046 0.14636
hsa-miR-423 MIR-423 -0.565 0.022 -0.53373 0.01661
mmu-miR-424 MIR-424 0 1 0.00000 0.50000
hsa-miR-425 MIR-425 0.444 0.085 -0.02134 0.46874
hsa-miR-020b MIR-020 0.12952 0.31630
hsa-miR-147 MIR-147 -0.02312 0.46613
hsa-miR-152N MIR-152 -0.12697 0.31969
hsa-miR-182* MIR-182 0.00336 0.49508
hsa-miR-196a MIR-196a-2 0.17554 0.25776
hsa-miR-199a MIR-199a-1-P -0.49145 0.02660
hsa-miR-199a* MIR-199a-1-P -0.52976 0.01741
hsa-miR-199-s MIR-199a-1-P -0.51851 0.01981
hsa-miR-199a* MIR-199a-2-P 0.43724 0.04517
hsa-miR-199-s MIR-199a-2-P 0.46991 0.03313
hsa-miR-200bN MIR-200b 0.389 0.136
hsa-miR-200c MIR-200c -0.027 0.92
hsa-miR-200cN MIR-200c -0.023 0.933
hsa-miR-203 MIR-203 0.646 0.007
hsa-miR-204 MIR-204 -0.281 0.292
hsa-miR-205 MIR-205 0.903 < 0.0001
hsa-miR-206 MIR-206 0.268 0.316
hsa-miR-208 MIR-208 0 1
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