LABORATORY CONDUCT AND SAFETY REGULATIONS (Indoor & Field Safety)

LABORATORY CONDUCT AND

SAFETY REGULATIONS(Indoor & Field Safety)

BiologyLake City High School

Mrs. Whittington

Standard B-1 The student will demonstrate an understanding of how scientific inquiry and technological design, including mathematical analysis, can be used appropriately to pose questions, seek answers, and develop solutions.

Standard

• B-1.9 • Use appropriate safety procedures

when conducting investigations.Safety regulations are established for

each laboratory by Federal, State, and School mandates.

Successful work in the laboratory and field involves not only mastery of concepts and techniques, but also knowing:

Objectives

• what the safety requirements are, • what equipment is available, and • when and how to use each piece of equipment• how to keep yourself and others safe and• how to respond to emergencies.

Safety regulations are established for each laboratory by Federal, State, and

School mandates.

Universal precautions

and basic lab safety

rules will be strictly

enforced

NON-COMPLIANCE• 1st violation: leave the activity

area until compliant–student may receive a zero for

work missed

• 2nd violation: leave the activity area for the day–student may receive a zero for

all work missed that day

NON-COMPLIANCE• 3rd violation: leave the activity

area permanently–student may receive a failing

lab grade

• *Severe violations will warrant immediate removal from the lab –student may receive a failing

lab grade

Lab Safety

STUDENT BEHAVIOR

• No horseplay (pushing, shoving, etc...) in the lab

• Always be aware of potential hazards & act

cautiously in the lab

Read, read, read• BEFORE beginning a

lab READ ALL safety precautions and lab

instructions..

Eating & Drinking• Food and drink of any

kind are strictly prohibited in the lab.

• Report all medical conditions (pregnancy, cardiac or respiratory disorders, allergies....)

• Only authorized people in the lab.

• All human secretions will be treated as biohazards

Stow books, backpacks, purses, etc... under the desk

APPAREL

• Lab aprons (tied in back)

• Long hair must be tied back

• Only closed toed shoes

Proper Clothing

Avoid floppyclothing!

Tie back loose hair!

Store belongingsout of the way!

Never take any lab materials out of the lab.• Lab materials are costly and

may be dangerous as well.• Taking them may result in

school discipline or even legal prosecution.

Eye and Face Safety

• No contact lenses during wet labs (when using chemicals or biohazards)

• Safety glasses during all wet labs

• Disposable gloves when working with hazards or chemicals

Eye Protection

Z87 Type Goggles

O

NO Food, Drink, Tobacco, Cosmetic use

Biology I Bell Ringer• You notice in the

lab instructions that you will be using test tubes. What are two rules that you know you will need to remember? (Hint: There may be more than 2, but you only need to write about two.)

Bell RingerFree Write Friday• Write at least 3

sentences on whatever you like.

• Examples: Tell me how your first week at school has been. Have any plans for the weekend?

Be prepared to turn in your bell ringers.

Today• Reminder Safety Contracts due today.

– You may turn it in late for a point reduction.• Anyone miss yesterday’s pre-test?

– Everyone must take the pre-test• Safety Quiz – Postponed due to printer

issues. Will be on Tuesday!• Put standards page in your folder. Initial the

safety indicator (B 1.9)• 1st Period – Acceptable Use Policy!

Assignment• You will be assigned a rule from the safety

contract.• Your mission is to create a sign that will

remind people to follow that rule.• Put your first and last name and the class

period in the upper right hand corner.– Must communicate the rule– Must be creative and neat– Must finish in class

Standard B-1 The student will demonstrate an understanding of how scientific inquiry and technological design, including mathematical analysis, can be used appropriately to pose questions, seek answers, and develop solutions.

Standard

• B-1.9 • Use appropriate safety procedures

when conducting investigations.Safety regulations are established for

each laboratory by Federal, State, and School mandates.

Successful work in the laboratory and field involves not only mastery of concepts and techniques, but also knowing:

Objectives

• what the safety requirements are, • what equipment is available, and • when and how to use each piece of equipment• how to keep yourself and others safe and• how to respond to emergencies.

Safety regulations are established for each laboratory by Federal, State, and

School mandates.

Glassware Safety

Special Caution-Handling Glassware

Lubricate glasssurfaces.

Protect hands.

NEVER forcethe glassware!

Special Caution-Handling Glassware

Check for any cracks, bubbles, or rough areas before using.

Broken Glass Disposal

UNCONTAMINATED broken glass

Fire Safety

• Watch for FLAMES from burners

• No unauthorized or unsupervised experiments

• Immediately report ALL – accidents – injuries– spills

FIRE SAFETY• Fire Extinguishers:• Aim nozzle at the

base of the fire• Pull pin and

squeeze handles together.

Fire Blanket:

• Pull the blanket out of the bottom using the black cord

• carefully cover the person to smother the fire

Fire Alarm:

• Pull the handle on the fire alarm to activate.

• Exit the building

• Emergency exit route: –Exit the lab and building. –Meet the teacher at the designated

site–Do NOT leave until you are released

Sharp Instruments

Sharps

Handle with Care!

Sharps Disposal

• Examples:–Needles–Razor blades–Scalpels–Scalpel blades

INSTRUCTIONS FOR WASTE CLEANUP

CHEMICAL WASTE DISPOSAL

Dilution is NOT the

solution to pollution!!!

LAB CLEAN-UP

• Glassware must be washed with soap & water & rinsed with distilled water

• Clean & disinfect your work area BEFORE leaving lab.

• Everything must be returned to its proper place

• Hands must be washed after removing gloves and before leaving the lab

WASTE DISPOSAL

•All waste has a specific disposal container

•Always use the properly labeled container

• If unsure ask the teacher.

Don’t pour ANYTHING down the sink unless you are

instructed to do so.

• LABEL ALL CONTAINTERS before putting anything in them with the following:– Date– Organism (or substance)– Medium (or chemical)– Your initials & class period

Biohazard Waste Labeling

• Know what waste products will be generated.

• Chemical Waste Labeling:–Obtain a clean, dry, empty

container. –Place a new CHEMICAL

WASTE label on front.

• DO NOT mix liquid & solid waste.

• Liquid waste SHOULD NOT be mixed unless it is of the same type.

• Fill container just below neck of the bottle.

Biohazard DisposalA BIOHAZARD IS ANTYING THAT

HAS COME INTO CONTACT WITH• BACTERIA

• FUNGI • VIRUSES

• BODILY FLUIDS

Plastic Biohazard Bag

• Examples: – Petri dishes– Specimen

containers– Pipette tips– contaminated

gloves

Biohazard Disposal Boxes

• Examples:Contaminated

–microscope slides–coverslips–broken glass–pipettes– test tubes

Cardboard Biohazard Box

• Examples:–Wooden probes–Toothpicks

Trash Receptacle

UNCONTAMINATEDgloves & paper towels

Chemical Safety

SAFETY CHECKLIST

• MSDS - Materials Safety Data Sheets chemical facts (ex: health hazards, first aid, disposal, etc...)

SOP FOR CHEMICAL SPILLS

• Immediately report all spills

• Proper safety apparel must be worn before clean-up of a spill– Lab coat– Goggles– Gloves

EmergencyShower:

• Pull the lever

• Stand under water for at least fifteen (15) minutes.

• Only pull lever if you need it, there is no drain close by

Eye Wash Station:

• Pop the caps off,

• Place the person's face over the fountain,

• Push the handle back Eyes must be held wide open,

• Flush at least fifteen (15) minutes

SOLID SPILLS• If solid is inorganic and without heavy

metals, sweep up & put in trash. • If solid is organic or has heavy metals,

sweep up & put in ziplock baggie.• Label & take to coordinator for

disposal. • Sweep up all broken glass & put in

broken glass container.

• First Aid: –Neutralize the danger if possible.

–If a student or teacher is injured, notify the office and they will send a first responder.

Mercury Thermometer Breaks• Mercury thermometers are no

longer used in school labs.• If a thermometer should break,

notify the teacher so that they can check to be sure it does not contain mercury.

Electrical Safety

Electrical SafetyDo not put anything other than an electrical plug into an outlet.

Keep electrical equipment and cords away from water or other liquids.

Check equipment for broken or frayed cords.

Lab Equipment• NEVER use or handle lab

equipment or other materials unless instructed to do so.

Pipetting

NOTWith your

mouth

MICROSCOPES• Hold with both hands

when transporting the scope

• Clean after every use

• Store with scanning objective in position & stage lowered

Funnel

Metric Ruler

Microscope

Flask

Test Tubes

Balance

Thermometer

Scissors

Scalpel

Forceps

Magnifying Glass

Graduated Cylinder

Beaker

Pipette

Medicine Dropper

Dissecting Tray

Microscope Slide

Cover slip

Dissecting Probes

Petri Dish

• You pour an acid down the sink, although your teacher did not tell you to.

Is this permissible?NO

Should you pour an acid into water or water into an acid?

Acid into water.

•Should you wear goggles when using a bunsen burner?

•YES

•You get HCL on your body, what should you do?

•Tell Your Teacher.•Rinse it off with water.

NO• You know you have salt in a

test tube and you don’t think heating it will hurt you or anyone else. Should you heat the salt even though your teacher didn’t tell you to?

• There is some salt in a beaker as part of an experiment you are doing. You want to make sure it is the salt, should you taste it?

• NO

• You are doing a lab with sugar water. It calls for 10 ml placed in a graduated cylinder. You goofed and poured in 15 ml. You decide to return 5 ml. back to the beaker of sugar water. Is this okay?

•Treat all microorganisms as if they are harmful even if they don’t cause diseases in humans. True or False?

• What is proper procedure when done with a lab?

• Clean up work area• Wash hands• Put equipment back where

you got it from

Safety Briefing Forms

• Fill out the student information form - which includes emergency information.

• Sign the Laboratory Conduct & Safety Regulations - Student Agreement.

Nature Trail Safety1. Be Aware of Your Surroundings

at all time.2. Look down and up as well as

around you.3. Watch your step.

Nature Trail Safety• You must walk with a partner.• If you get separated from your

partner, return to the bench area.• Stay within hearing range of other

groups.

Nature Trail Safety• When looking under logs or other

materials, turn the object so that anything under it can escape AWAY from you.

• Do not try to roll over large objects.• Do not handle spiders, unfamiliar insects,

lizards, or animals without supervision.

Nature Trail Safety• Never put your hands or feet in areas that

you cannot see clearly (piles of leaves, holes, under trees, etc.)

• Use a long branch, walking stick, or other tool to clear away deep leaves or turn over small logs.

Nature Trail Safety• You must wear appropriate

clothing. Sturdy shoes (not sandals, bedrooms shoes, etc.), long pants, and a shirt with long sleeves or a jacket is best.

Nature Trail Safety• Listen for the whistle!• Return to meeting spot

immediately.• No horse play (climbing trees,

running, climbing under or on fallen trees, etc.)

Nature Trail Safety• Report any injuries, stings, rashes,

etc. to the teacher.• When you get home remove clothing

and check thoroughly for ticks.• Ticks should be pulled gently from

the skin with tweezers.

Plant Safety inSouth Carolina

Poison Ivy• Identifying Characteristics:

–3 leaflets–Climbing vine–Bright red leaves in the fall

• Danger/Caution:–Skin irritation for some people

Poison Oak• Identifying Characteristics:

–3 leaflets–Smaller shrub like–Leaves have oak-like shaped lobes.

• Danger/Caution:–Skin irritation for some people

Poison Sumac

• Identifying Characteristics:–Compound leaf–Orange-red stem w/ green leaves

• Danger/Caution:–Skin irritation for some people

Cow Itch / Trumpet Creeper

• Identifying Characteristics:–Climbing vine–Reddish trumpet shaped flowers

• Danger/Caution:–Skin irritation for some people

Virginia Creeper• Identifying Characteristics:

–Climbing vine–5 leaflets–Reddish in the fall

• Danger/Caution:–Flowers & Leaves poisonous to eat

Yellow Jasmine• Identifying Characteristics:

–Climbing vine–Yellow flowers–SC State Flower

• Danger/Caution:–Flowers & Leaves poisonous to eat

Rhododendron / Mtn. Laurel

• Identifying Characteristics:–Looks like the azaleas that people

have in their yards. Same family.–Shrub with small oblong dark green

leaves with flowers (white to red)• Danger/Caution:

–Leaves & flowers are an internal poison if eaten.

Rattlebox / Crotalaria

• Identifying Characteristics:–Weedy plant w/ yellow flower–Seed pods dry and rattle

• Danger/Caution:–Seeds are poisonous if eaten and can kill

a horse.

Rattlebox

Rattlebox

Pokeweed (Pokeberry)• Identifying Characteristics:

–Tall broadleaf weed. Head high–Common in waste areas (vacant

lots, etc.)• Danger/Caution:

–Roots are poisonous–Leaves are poisonous if not

cooked correctly.

Nightshade (Deadly Nightshade)

• Identifying Characteristics:–Flowers look like the flowers on tomato

plants.–Small fruit that look like little yellow

tomatoes–Soft, hairy spines on stems

• Danger/Caution:– Internal poison if any part is eaten.

Nightshade

Black Cherry• Identifying Characteristics:

–Spindly shrub/small tree–Little green berries which ripen to a

dark color• Danger/Caution:

–The fruit is okay but the seeds are poisonous and contain cyanide.

Black Cherry

Azalea• Identifying Characteristics:

–Common flowering shrub around homes.

–White, pink, salmon, and/or red flowers

• Danger/Caution:–Leaves and flowers are poisonous if

eaten.

Daffodils

• Identifying Characteristics:–Yellow to white trumpet (cup & saucer)

shaped flowers that grow from a bulb.• Danger/Caution:

–Bulbs are poisonous if eaten

Wisteria

• Identifying Characteristics:–Climbing vine with grape-like clusters

of purple flowers.–Velvety, long seed pods

• Danger/Caution:–Seeds are poisonous if eaten

Chinaberry Tree

• Identifying Characteristics:–May be found as a shrub or a tree–Green berries that turn a waxy (bone)

color and become squishy.• Danger/Caution:

–Berries can be poisonous if eaten.

Boxwood

• Identifying Characteristics:–Evergreen shrub with small leaves

and thin woody stems. Often found around homes.

• Danger/Caution:–Leaves are poisonous if eaten.

Poinsettia• Identifying Characteristics:

–Green and red or white leaves that mimic flowers.

–Sold at Christmastime but there is a small variety that also grows wild in SC.

• Danger/Caution:–Leaves are poisonous if eaten.

Mistletoe

• Identifying Characteristics:–Evergreen plant that grows as a

parasite in trees.–White, waxy berries

• Danger/Caution:–Leaves and berries are poisonous

Holly• Identifying Characteristics:

–Waxy, shiny green leaf top with spines

–Varies from a shrub to tree size–Red berries

• Danger/Caution:–Berries are poisonous if eaten.

Jimsonweed (Thorn Apple)• Identifying Characteristics:

–Small, weedy plant found commonly in waste areas

–White flowers–Spiny fruit pod

• Danger/Caution:–All parts are extremely

poisonous if eaten.

Thorn Apple