Lab 19 –Pituitary Gland Pituitary GlandLab 19 –Pituitary Gland A560 –Fall 2015 I. Introduction...

PituitaryGlandLab19– PituitaryGland

A560– Fall2015

I. IntroductionII. LearningObjectivesIII. SlidesandMicrographs

A. GeneralstructureB. Parts1. AnteriorPituitarya. Divisionsi. Parsdistalisii. Parsintermediaiii. Parstuberalis

b. Cellsi. Chromophobesii. Chromophils1) Acidophils2) Basophils

2. PosteriorPituitarya. Divisionsi. Infundibulumstalkii. Parsnervosa

b. Cellsi. Pituicytes

IV. SummaryFig20‐2,Junqueira,13th ed.

Lab19– PituitaryGlandA560– Fall2015

I. IntroductionII. LearningObjectivesIII. SlidesandMicrographs

A. GeneralstructureB. Parts1. AnteriorPituitarya. Divisionsi. Parsdistalisii. Parsintermediaiii. Parstuberalis

b. Cellsi. Chromophobesii. Chromophils1) Acidophils2) Basophils

2. PosteriorPituitarya. Divisionsi. Infundibulumstalkii. Parsnervosa

b. Cellsi. Pituicytes

IV. Summary

Slide128:Pituitary,H&E

anteriorpituitary

posteriorpituitary

parsintermedia

Lab19– PituitaryGlandA560– Fall2015

I. IntroductionII. LearningObjectivesIII. SlidesandMicrographs

A. GeneralstructureB. Parts1. AnteriorPituitarya. Divisionsi. Parsdistalisii. Parsintermediaiii. Parstuberalis

b. Cellsi. Chromophobesii. Chromophils1) Acidophils2) Basophils

2. PosteriorPituitarya. Divisionsi. Infundibulumstalkii. Parsnervosa

b. Cellsi. Pituicytes

IV. Summary

Slide128(Hi‐Res):Pituitary,H&E

anteriorpituitary

posteriorpituitary

parsintermediaw/remnantsofRathke’s pouch

Lab19– PituitaryGlandA560– Fall2015

I. IntroductionII. LearningObjectivesIII. SlidesandMicrographs

A. GeneralstructureB. Parts1. AnteriorPituitarya. Divisionsi. Parsdistalisii. Parsintermediaiii. Parstuberalis

b. Cellsi. Chromophobesii. Chromophils1) Acidophils2) Basophils

2. PosteriorPituitarya. Divisionsi. Infundibulumstalkii. Parsnervosa

b. Cellsi. Pituicytes

IV. Summary

Slide103:AnteriorPituitary,H&E

anteriorpituitary

posteriorpituitary

parsintermedia

Lab19– PituitaryGlandA560– Fall2015

I. IntroductionII. LearningObjectivesIII. SlidesandMicrographs

A. GeneralstructureB. Parts1. AnteriorPituitarya. Divisionsi. Parsdistalisii. Parsintermediaiii. Parstuberalis

b. Cellsi. Chromophobesii. Chromophils1) Acidophils2) Basophils

2. PosteriorPituitarya. Divisionsi. Infundibulumstalkii. Parsnervosa

b. Cellsi. Pituicytes

IV. Summary

Slide122:Pituitary,H&E

anteriorpituitary

posteriorpituitary

parsintermedia

Lab19– PituitaryGlandA560– Fall2015

I. IntroductionII. LearningObjectivesIII. SlidesandMicrographs

A. GeneralstructureB. Parts1. AnteriorPituitarya. Divisionsi. Parsdistalisii. Parsintermediaiii. Parstuberalis

b. Cellsi. Chromophobesii. Chromophils1) Acidophils2) Basophils

2. PosteriorPituitarya. Divisionsi. Infundibulumstalkii. Parsnervosa

b. Cellsi. Pituicytes

IV. Summary

Slide74:Pituitary,GomoriTrichrome

anteriorpituitary

posteriorpituitary

Lab19– PituitaryGlandA560– Fall2015

I. IntroductionII. LearningObjectivesIII. SlidesandMicrographs

A. GeneralstructureB. Parts1. AnteriorPituitarya. Divisionsi. Parsdistalisii. Parsintermediaiii. Parstuberalis

b. Cellsi. Chromophobesii. Chromophils1) Acidophils2) Basophils

2. PosteriorPituitarya. Divisionsi. Infundibulumstalkii. Parsnervosa

b. Cellsi. Pituicytes

IV. Summary

Slide128(Hi‐Res):Pituitary,H&E

anteriorpituitary

posteriorpituitarypars

intermedia

parsdistalis

parstuberalis

Lab19– PituitaryGlandA560– Fall2015

I. IntroductionII. LearningObjectivesIII. SlidesandMicrographs

A. GeneralstructureB. Parts1. AnteriorPituitarya. Divisionsi. Parsdistalisii. Parsintermediaiii. Parstuberalis

b. Cellsi. Chromophobesii. Chromophils1) Acidophils2) Basophils

2. PosteriorPituitarya. Divisionsi. Infundibulumstalkii. Parsnervosa

b. Cellsi. Pituicytes

IV. Summary

Slide122:Pituitary,H&E

the pars distalis accounts for 75% of the anterior pituitary (adenohypophysis); it is composed primarily ofcords of endocrine cells interspersed with fenestrated capillaries; some reticular CT is also present

Lab19– PituitaryGlandA560– Fall2015

I. IntroductionII. LearningObjectivesIII. SlidesandMicrographs

A. GeneralstructureB. Parts1. AnteriorPituitarya. Divisionsi. Parsdistalisii. Parsintermediaiii. Parstuberalis

b. Cellsi. Chromophobesii. Chromophils1) Acidophils2) Basophils

2. PosteriorPituitarya. Divisionsi. Infundibulumstalkii. Parsnervosa

b. Cellsi. Pituicytes

IV. Summary

Slide128(Hi‐Res):Pituitary,H&E

anteriorpituitary

posteriorpituitary

parsintermedia

the pars intermedia contains basophilic cells between the pars distalis of the anterior pituitary and thepars nervosa of the posterior pituitary; eosinophilic colloid‐filled cysts represent remnants of thehypophyseal pouch (Rathke’s pouch); functional significance of this region is uncertain

Lab19– PituitaryGlandA560– Fall2015

I. IntroductionII. LearningObjectivesIII. SlidesandMicrographs

A. GeneralstructureB. Parts1. AnteriorPituitarya. Divisionsi. Parsdistalisii. Parsintermediaiii. Parstuberalis

b. Cellsi. Chromophobesii. Chromophils1) Acidophils2) Basophils

2. PosteriorPituitarya. Divisionsi. Infundibulumstalkii. Parsnervosa

b. Cellsi. Pituicytes

IV. Summary

Slide74:Pituitary,GomoriTrichrome

anteriorpituitary

posteriorpituitary

Lookhereforsecretorycells

Lab19– PituitaryGlandA560– Fall2015

I. IntroductionII. LearningObjectivesIII. SlidesandMicrographs

A. GeneralstructureB. Parts1. AnteriorPituitarya. Divisionsi. Parsdistalisii. Parsintermediaiii. Parstuberalis

b. Cellsi. Chromophobesii. Chromophils1) Acidophils2) Basophils

2. PosteriorPituitarya. Divisionsi. Infundibulumstalkii. Parsnervosa

b. Cellsi. Pituicytes

IV. Summary

Slide74:Pituitary,Gomori Trichrome

chromophobe

acidophil

basophil

Lab19– PituitaryGlandA560– Fall2015

I. IntroductionII. LearningObjectivesIII. SlidesandMicrographs

A. GeneralstructureB. Parts1. AnteriorPituitarya. Divisionsi. Parsdistalisii. Parsintermediaiii. Parstuberalis

b. Cellsi. Chromophobesii. Chromophils1) Acidophils2) Basophils

2. PosteriorPituitarya. Divisionsi. Infundibulumstalkii. Parsnervosa

b. Cellsi. Pituicytes

IV. Summary

Slide122:Pituitary,H&E

chromophobes are identified by their pale‐staining cytoplasm and absence ofsecretory granules; they represent several different types of cells, including stem cellsand degranulated cells

acidophil basophil

Lab19– PituitaryGlandA560– Fall2015

I. IntroductionII. LearningObjectivesIII. SlidesandMicrographs

A. GeneralstructureB. Parts1. AnteriorPituitarya. Divisionsi. Parsdistalisii. Parsintermediaiii. Parstuberalis

b. Cellsi. Chromophobesii. Chromophils1) Acidophils2) Basophils

2. PosteriorPituitarya. Divisionsi. Infundibulumstalkii. Parsnervosa

b. Cellsi. Pituicytes

IV. Summary

Slide74:Pituitary,Gomori Trichrome

acidophils have strongly‐staining acidophilic (eosinophilic) cytoplasm; they secrete growthhormone and prolactin (somatotrophs [about half of total cells] and lactotrophs, respectively)

Lab19– PituitaryGlandA560– Fall2015

I. IntroductionII. LearningObjectivesIII. SlidesandMicrographs

A. GeneralstructureB. Parts1. AnteriorPituitarya. Divisionsi. Parsdistalisii. Parsintermediaiii. Parstuberalis

b. Cellsi. Chromophobesii. Chromophils1) Acidophils2) Basophils

2. PosteriorPituitarya. Divisionsi. Infundibulumstalkii. Parsnervosa

b. Cellsi. Pituicytes

IV. Summary

Slide74:Pituitary,Gomori Trichrome

basophils (less numerous) have strongly‐staining basophilic cytoplasm; they include:corticotrophs (ACTH and LPH), gonadotrophs (FSH and LH/ICSH), and thyrotrophs (TSH)

Lab19– PituitaryGlandA560– Fall2015

I. IntroductionII. LearningObjectivesIII. SlidesandMicrographs

A. GeneralstructureB. Parts1. AnteriorPituitarya. Divisionsi. Parsdistalisii. Parsintermediaiii. Parstuberalis

b. Cellsi. Chromophobesii. Chromophils1) Acidophils2) Basophils

2. PosteriorPituitarya. Divisionsi. Infundibulumstalkii. Parsnervosa

b. Cellsi. Pituicytes

IV. Summary

Slide128(Hi‐Res):Pituitary,H&E

anteriorpituitary

posteriorpituitary

parsnervosa

infundibulumstalk

Lab19– PituitaryGlandA560– Fall2015

I. IntroductionII. LearningObjectivesIII. SlidesandMicrographs

A. GeneralstructureB. Parts1. AnteriorPituitarya. Divisionsi. Parsdistalisii. Parsintermediaiii. Parstuberalis

b. Cellsi. Chromophobesii. Chromophils1) Acidophils2) Basophils

2. PosteriorPituitarya. Divisionsi. Infundibulumstalkii. Parsnervosa

b. Cellsi. Pituicytes

IV. Summary

Slide128(Hi‐Res):Pituitary,H&E

pituicytes, majority of nuclei seen, are glial cells that support the unmyelinated axons that compose majorityof posterior pituitary (neurohypophysis); axons release ADH and oxytocin (nothing produced in posteriorpituitary); Herring bodies, faintly‐staining eosinophilic accumulations of secretory products in axon terminals(not to be confused with capillaries), might be seen

Lab19– PituitaryGlandA560– Fall2015

I. IntroductionII. LearningObjectivesIII. SlidesandMicrographs

A. GeneralstructureB. Parts1. AnteriorPituitarya. Divisionsi. Parsdistalisii. Parsintermediaiii. Parstuberalis

b. Cellsi. Chromophobesii. Chromophils1) Acidophils2) Basophils

2. PosteriorPituitarya. Divisionsi. Infundibulumstalkii. Parsnervosa

b. Cellsi. Pituicytes

IV. Summary

EMstoExamine

Fig20‐7:Ultrastructureofsomatotropic cells