It’s more than just colors, my seminar

“It’s more than just colors!”

Finding novel uses for natural dyes from Philippine indigenous plants

Therese Julienne T. MedinaMS MBB

http://farm3.static.flickr.com

• Introduction– Natural Dyes: A Definition– History of natural dyes and dyeing– Classic examples of dye-yielding plants– Traditional uses of natural dyes– The Rise of Synthetic Dyes: Implications– Ideals of the 21st century: “Going Natural”– Recent Studies: Novel uses of Natural Dyes

• In the Philippine Context: A Proposal– Natural Dyes in the Philippines: The New

Challenge– Philippine Indigenous Dye-yielding Plants– Natural Dyes R&D: Rediscovering dye-yielding

plants• Anatomical and Histochemical Procedures• Extraction and Constituents Identification• Bioassay studies for therapeutic potential• Elaboration of natural dye production: role of

biotechnology

Natural Dyes: A definition

• COLOR - a molecule that absorbs certain wavelengths of visible light and transmits or reflects others.

• COLORANT - the activity of the molecule when applied to a substance.

• DYES - materials that gives color to objects such as yarn, food, paper, cosmetics, and cloth.

• NATURAL DYES/ BIOCOLORANTS - coloring agents obtained from nature or biological sources: plants, animals, fungi, and bacteria.

Natural Dyes: A definition

History of natural dyes and dyeing

http://traditions.cultural-china.com/en/15Traditions1024.html

http://indusvalleycivilizationf.blogspot.com/2010/03/rilli-quilt-ancient-handicraft-of-indus.html

www.ancientresource.com/lots/egyptian.html

History of natural dyes and dyeing

http://www.immortalitea.com/Mulberry_history.htm www.dawns-light.co.kr/en/page11.htmlhttp://northstoke.blogspot.com/2009/11/indigo-dyeing.html

flickr.com/photos/35237098989@N01/22767364/

Classic examples of dye-yielding plants

Rubia tinctorum

http://www.fibrecrafts.com/resource/fact_file/dyes/fibrecrafts_natural_dyes.asp

Indigofera tinctoria

http://www.fibrecrafts.com/resource/fact_file/dyes/fibrecrafts_natural_dyes.asp

Anthemis tinctoria

:www.zelenhoz.com/n/f4.htmhttp://www.fibrecrafts.com/resource/fact_file/dyes/fibrecrafts_natural_dyes.asp

Quercus infectoria

www.elizabethancostume.nethttp://www.fibrecrafts.com/resource/fact_file/dyes/fibrecrafts_natural_dyes.asp

Traditional uses of natural dyes

http://www.sagegrovecoven.com www.himfr.com/d-p114221535560486025-Natural_Dyes/

The Rise of Synthetic Dyes: Implications

William Henry Perkin• Accidentally produced and

discovered MAUVE, the first synthetic dye

• More synthetic dyes were created after his discovery

• Natural dye use steadily declined

http://www.soci.org/About-Us/About-SCI/History/Notable-Chemists

The Rise of Synthetic Dyes: Implications

http://ecologicalartist.wordpress.com/2009/07/http://goldencarla.typepad.com/carla_goldens_get_healthi/golden-spectrum/

Ideals of the 21st century:

“Going Natural”

• Natural dyes as natural food grade biocolorants

Recent Studies: Novel uses of Natural Dyes

• Natural dyes in food industry: as food preservatives, quality control markers, and nutritional supplements

anthocyanins

carotenoids

riboflavin

• Natural dyes as therapeutic agents

• Natural dyes as photosensitizers for dye-sensitized solar cell

Hao et.al., 2005. Natural dyes as photosensitizers for dye-sensitized solar cell

In the Philippine Context: A Proposal

Natural Dyes in the Philippines:The New Challenge

• Revival of natural dye industries– Abra and Ifugao, re-establish plantations of dye-

yielding trees – Natural dye R&D Program

• CHALLENGE: establish new applications and techniques for the improvement of man’s health and wellness

Philippine Indigenous Dye-yielding Plants

• Indigofera tinctora, Terminalia catappa, Curcuma longa, Bixa orellana, Caesalpinia sappan

http://www.djibnet.com/photo/fabaceae/indigofera-tinctoria-1655002816.html http://fr.academic.ru/dic.nsf/frwiki/1614018

http://otvet.mail.ru/question/29923774/

• Acacia farnesiana, Michelia chanpaca, Hibiscus sabdariffa, Leucaena leucocephala, Mirabilis jalapa

Philippine Indigenous Dye-yielding Plants

http://www.desertfolia.com/index.php?mact=News,cntnt01,detail,0&cntnt01articleid=5&cntnt01returnid=67

www.jungleseeds.com/SeedShop/TropicalFruit.htmwww.backyardnature.net/yucatan/bean-fam.htmwww.natureproducts.net/.../Mirabilis.html

Natural Dyes R&D: Rediscovering dye-yielding plants

• Anatomical and Histochemical Procedures

http://www.indianjournals.com/ijor.aspx?target=ijor:apps&volume=15&issue=1&article=040http://www.flickr.com/photos/nikyhughes/395720897/

• Extraction and Constituents Identification

http://www.biomedcentral.com/1472-6750/8/25/figure/F6?highres=yhttp://www.knaes.affrc.go.jp/sporf/no14/m4.html

• Extraction and Constituents Identification

Color Generic name (chemical group)

Examples

Blue and Purple Dyes

IndicanAnthocyanins

IndigoCyanidin

Red dyes AnthraquinoneNeoflavonoids

AlizarinBrazilin

Yellow Dyes FlavonesFlavonol

LuteotinMorin

Black and Brown Dyes

Tanins Gallic acid Catechin

• Bioassay studies for therapeutic potential

www.fgsc.net/fgn39/ciufet.html

Microbial assay for antimicrobial effect against:Escherichia coliBacillus subtilisKlebsiella pneumoniaeProteus vulgarisPseudomonas aeruginosa

• Elaboration of natural dye production: role of biotechnology

http://www.ars-fla.com/Mainpages/Bio-Reactor/bioreactor.html

SUMMARY• Natural dyes:

– Were used thousands of years ago for textiles, foods, and cosmetics

– Were replaced by synthetic dyes in the market (because of their cheaper cost)

– are being revived and studied for new applications (biocolorant, anti-microbial agent, photosensitizer)

– In the Philippines, R&D programs on natural dyes are established.

There is a need for:

• proper collection,• documentation,• assessment and characterization of dye

yielding plants and their dyes, and• research to overcome the limitations of

natural dyes

References• Abello, M. 2007. Of indigo, talisay, and natural dying technology. Agriculture magazine.

11(12):26-27.• Chattopadhyay, P., S. Chatterjee, and SK. Sen. 2008. Biotechnological potential of natural

food grade biocolorants. African Journal of Biotechnology. 7(17):2972-2985. • Gokhale, SB., AU. Tatiya, SR. Bakliwal, and RA. Fursule. 2004. Natural dye yielding plants in

India. Natural Product Radiance. 3(4):228-234.• Quinto, J.E. 1992. Anatomical Study of Some Dye Containing Species of Family Verbenaceae

and Stability of their Dyes. Unpublished masters’ thesis, UPLB.• Rosenberg, E. 2008. Characterization of historical organic dyestuffs by liquid

chromatography- mass spectrometry. Anal Bioanal Chem 391:33-57.• Singh R., A. Jain, S. Panwar, D. Gupta, and SK. Khare. 2005.Dyes and Pigments. 66(2):99-

102.• Siva, R. 2007. Status of natural dyes and dye yielding plants in India. Current Science. 92(7):

916-925

http://www.plazanimal.cl/cms/index.php

http://www.ars-fla.com/Mainpages/Bio-Reactor/bioreactor.htmlhttp://www.ars-fla.com/Mainpages/Bio-Reactor/bioreactor.htmlhttp://www.knaes.affrc.go.jp/sporf/no14/m4.htmlhttp://www.biomedcentral.com/1472-6750/8/25/figure/F6?highres=yhttp://www.indianjournals.com/ijor.aspx?target=ijor:apps&volume=15&issue=1&article=040http://www.flickr.com/photos/nikyhughes/395720897/

http://www.desertfolia.com/index.php?mact=News,cntnt01,detail,0&cntnt01articleid=5&cntnt01returnid=67www.jungleseeds.com/SeedShop/TropicalFruit.htmwww.backyardnature.net/yucatan/bean-fam.htmwww.natureproducts.net/.../Mirabilis.html

http://www.djibnet.com/photo/fabaceae/indigofera-tinctoria-1655002816.htmlhttp://fr.academic.ru/dic.nsf/frwiki/1614018

http://otvet.mail.ru/question/29923774/

http://traditions.cultural-china.com/en/15Traditions1024.html

http://indusvalleycivilizationf.blogspot.com/2010/03/rilli-quilt-ancient-handicraft-of-indus.htmlwww.ancientresource.com/lots/egyptian.html

Thank You!

It’s more than just colors, my seminar

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