Great new Krebs cycle (Citric Acid cycle) movie…

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Lecture 7 Slides

Great new Krebs cycle (Citric Acid cycle) movie…

fig 16-13

http://www.youtube.com/watch?v=A1DjTM1qnPM

Krebs cyclefeatures andfunctions…

fig 16-13

CH3-CO

O-

CH3-CO

acetate

acetyl group

S-CoA

AcCoA

acetyl coenzyme Aor

acetyl-CoA

CH3-CO

it’s all about acetate…

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fig16-1

all roads lead to acetyl-CoA…

fig16-1

electrons from acetyl groups

fig16-1

energy from electrons

fig16-3

This is HS-CoA, or CoA-SH, CoA, or coenzyme A

fig16-3

This is HS-CoA, or CoA-SH, CoA, or coenzyme A

CoA-SH, or HS-CoA, or CoA

fig16-2

where the acetyl groups come from

pyruvate dehydrogenase: PDH

fig 14-13

Thiamine pyrophosphate (TPP): an activated carbon

fig 14-13

TPP as a carbanionnucleophile in pyruvate metabolism

fig16-4

The “business end” of PDH

Lipoic acid cofactor of PDH E2

fig16-5

The PDH complex approx. 4.5 x 106 MW

fig16-5

The PDH complex

fig16-6 (old)

The PDH catalyticcycle

oxidation of pyruvate

Several reactions, one basic location

fig16-6

Please read and LEARN

“The Name Game”

fig16-7

The:Krebs cyclecitric acid cycleTCA cycle

fig 16-8

Citrate synthase and its substrates…

pg 622

Getting fresh carbons into the act

why it’s called the citric acid cycle

pg 622

making citrate asymmetric

remember this for later…

fig16-10

an iron-sulfer cluster in aconitase

pg 624 (less fancy picture)

electrons!

NADH is the main carrier ofKrebs cycle-derived electrons

pg 625

more electrons!

note CoA-SH has re-entered the picture

pg 626

direct production of a high energy phosphate

but not ATP…instead it’s GTP

pg 626

direct production of a high energy phosphate

but not ATP…instead it’s GTP

pg 628

electrons to FAD instead of NAD+

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The business end of FAD or FMN

isoalloxazinering

pg 628

electrons to FAD instead of NAD+

pg 628

a sensible inhibitor of succinate dehydrogenase

INHIBITOR

old pg 612

Hydration of fumarate

pg 628

Hydration of fumarate

pg 628

high specificity exemplified by substrates and productsof fumarase

substrate

product

not asubstrate

pg 628

high specificity exemplified by substrates and productsof fumarase

not aproduct

substrate

product

pg 628

the final electron removal

Krebs cyclefeatures andfunctions…

fig 16-13