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Genetic Engineering:How and why scientists manipulate DNA in living cells
http://farm3.static.flickr.com
Karyotype
chart of chromosome
pairs used to identify
unusual #s of chromosomes
**Down syndrome (Trisomy 21) – 3 chrom. at pair 21 Turner’s syndrome – only one X – sterile female XO Klinefelter’s syndrome – extra X (male:XXY-sterile)
Karyotype
http://users.rcn.com/jkimball.ma.ultranet/BiologyPages/D/Down.gif
http://www.biologyreference.com/images/biol_04_img0412.jpg
Nondisjunction – causes these unusual #s of chromosomes; chromosomes fail to separate during meiosis
http://www.medgen.ubc.ca/wrobinson/backup/mosaic/images/nondis_m2.gif
http://www.ratsteachgenetics.com/Genetics_quizzes/Lecture%207/7q1.jpg
Selective breeding We choose desired traits & breed plants and animals to have these traits *Takes decades Examples: popcorn, dog breeds, cows produce 3x more milk than 50 years ago, bigger peaches,
apple varieties
http://img.dailymail.co.uk/i/pix/2008/02_02/CaulliDM_468x518.jpg
Selective breeding – 2 methods 1. Hybridization – cross dissimilar individuals
to bring together best traits Ex. Crossing disease-resistant apple with high
yield apple = apple tree that
produces a lot and requires
less pesticides
http://www.biologyreference.com/images/biol_02_img0235.jpg
2. Inbreeding – done to maintain traits *because genetics are so close, often allows
expression of recessive allele Study revealed that20,000 boxers genetically look like 70-deaf dalmations &white boxers, heart disease,hip dysplasia
http://www.boxerbuddies.org/Boxer%20Info/oscar10.jpg
Test cross A way of determining genotype Cross a known individual -(homozygous recessive) with an unknown –
homozygous dominant or heterozygousIf unknown is homozygous dominant, all offspring will
show dominant traitIf unknown is heterozygous, some will show dominant
trait, some show recessive traitComplete Problem Solving Lab 13.1 p.339
http://farm3.static.flickr.com/2023/2503367977_4a5dc2345a.jpg?v=0
Manipulating DNA
Process How
Cut DNARestriction Enzymes
(like scissors)
Sort DNA Gel Electrophoresis
Analyze DNA DNA fingerprint
1. Cut DNA: Restriction enzymes DNA is too large to be analyzed Highly specific restriction enzymes from bacteria cut
DNA into precise pieces between certain base pairs Ex. EcoRI can only recognize GAATTC – it cuts between
the G and A Leaves “sticky ends” – single-stranded overhangs
used to bond “stick” to another DNA stand cut with the same restriction enzyme
Practice p.343
Restriction enzymes
http://www.mhhe.com/biosci/esp/2001_gbio/folder_structure/ge/m6/s1/assets/images/gem6s1_1.jpg
2. Sort DNA: Gel Electrophoresis
http://www.bio.miami.edu/~cmallery/150/gene/c7.20.8.electrophoresis.jpg
p. 346
1.Insert restriction-enzyme-cutDNA into gel 2. Add electricity.
Shorter fragments move farther
3. Result: DNA fingerprint
3. Analyze DNA: DNA fingerprinting
*Sample from
blood, hair, skin,
semen
*Use PCR
(polymerase
chain reaction)
to make more
http://dnamazing.com/wp-content/uploads/2007/08/dnabasics2.JPG
Can you match the evidence with the suspect?
http://images.encarta.msn.com/xrefmedia/sharemed/targets/images/pho/t235/T235690A.jpg
PS lab 13.3 p.353
Human Genome Project (1990-2003)
*Sequenced all 3 billion base pairs of human DNA and identified
all human genes.
*Used to determine
carriers for diseases
& develop gene
therapy
http://www.sanger.ac.uk/Info/Press/gfx/030414_hgp_300.jpg
Product of
Human Genome:
chromosome
maps
http://www.mhhe.com/biosci/genbio/maderbiology7/graphics/mader07b/online_vrl/images/0287l.jpg
Genetic engineering:
faster than selective
breeding. Cut DNA
from one organism,
insert into another. Recombinant
DNA: combining
DNA from different
sources
http://campus.queens.edu/faculty/jannr/Genetics/images/dnatech/bx15_01.jpg
Transgenic organisms Contain genes from other species Insert recombinant DNA into a new host Use plasmid – circle of DNA in bacteria Insert recombinant DNA into plasmid, reinsert
plasmid into bacteria- bacteria clones DNA as it divides (mitosis); get lots of copies of DNA
Used to produce insulin to treat diabetes, human growth hormone, blood-clotting factors for hemophiliacs, potentially even cancer-fighting molecules (interleukin-2 and interferon)
http://www.bio.miami.edu/~cmallery/150/gene/c7.20.4.insulin.jpg
Recombinant bacteria manufacture insulin
http://www.e-ishraq.com/v4/images/6_3.gif
Cloning Uses a single somatic
cell from an adult organism to grow an entirely new genetically identical organism
http://static.howstuffworks.com/gif/cloning-sheep.gif
Uses of transgenic animals
Insert spider genes into cells of lactating goats – manufacture silk along with milk = light, tough, flexible material military uniforms, medical sutures
30% of US milk produced by genetically modified (GM) cows Pigs GM to producehigh levels of lean meat
http://www.scienceprogress.org/wp-content/uploads/2009/01/goat_591.jpg
http://upload.wikimedia.org/wikipedia/en/thumb/8/88/GoldenRice-WhiteRice.jpg/800px-GoldenRice-WhiteRice.jpg
http://www.bio.davidson.edu/courses/GENOMICS/2008/Waters/TIME.jpg
Transgenic plants: Golden Rice – GM to have high levels of beta-carotene – essential for health, eye sight
Gene therapy Absent or faulty genes replaced by normal
working genes – allows body to make proteins or enzymes needed, eliminating disorder
Used to treat SCID (severe combined immunodeficiency syndrome)
Trials for sickle-cell, cystic fibrosis, and hemophilia treatments are going on now
http://library.thinkquest.org/28000/media/genetherapy/l_gene.therapy-ms.gif
Future possibilities?
http://ec1.images-amazon.com/images/I/51EbEYn8hAL.jpg
Implications? Ethical issues? Patenting life Genetic ownership GINA (Genetic Information
Nondiscrimination Act) passed in 2008 Protects Americans against discrimination from
employers or insurance companies based on genetic information
http://media-2.web.britannica.com/eb-media/64/47664-004-7088EE3D.jpg
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