Fruit Tree Clean Plant Center WSU - IAREC. New personnel: James Susaimuthu supervises virus...

Fruit Tree Clean Plant CenterWSU - IAREC

New personnel:James Susaimuthu

• supervises virus indexing

Sam Hood• manages growth facilities

Terri Reddout• assists FTCPN management

Martin Joseph• directs tissue culture program

Infrastructure improvements:• Greenhouse renovations

• Complete• Biosecurity • Improved environmental control

• Relocate thermal therapy facility • Near complete• Biosecurity

• New greenhouse (industry funded)• Design complete• Replaces deteriorated structure• Biosecurity

Asbestos bench tops

Gravel floor

Deteriorated glazing

Replacement quarantine greenhouse• designed

• major components purchased

Upgraded irrigation system

Budwood distributions:• Domestic releases 27,277• International releases 1,618

• Representing 13 countries

Selections received:• Proprietary 55• Foreign 28• Public 17

• Indexing only 72

• Heat therapy 20

Thermal therapy•130 selections going through heat therapy• Includes those received in previous years

Current testing cycle in progress:•Woody indexing tests 20,000•Herbaceous indexing (Mar) 600•Molecular assays 1,500•ELISAs 1,500

Malus: Required Actual

Woody indexing 5 X 4 reps each 5 X 4 reps each

Herbaceous indexing

4 4

Field indexing 4 4

PCR / RT-PCR 2 5 + 4 to confirm

ELISA - -

Stone fruit:Woody indexing 8 X 4 reps each 8 X 4 reps each

Herbaceous indexing

4 4

Field indexing 1 1

PCR / RT-PCR 3 7 + 3 to confirm

ELISA 4 4

Current FTCPC research activities:

CVA – capillovirus• ~ 50% of cherry selections infected• Likely difficult to remove via thermal therapy• Impact unknown

Apple green crinkle disease• 3 cropping years is required protocol• Identify pathogen associated with disease

Rusty mottle / Twisted leaf• Genetically complex family of viruses• May be able to eliminate some woody indexing