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FIRST ANNUAL REPORT
28/02/2020 DELIVERABLE 7.8 – WORK PACKAGE 7
Dissemination level: Public
Ref. Ares(2020)1261111 - 28/02/2020
FIRST ANNUAL REPORT
Grant Agreement number: 813439 — BREAK BIOFILMS — H2020-MSCA-ITN-2018
FIRST ANNUAL REPORT
D E L I V E R A B L E 7 . 8 – W O R K P A C K A G E 7
DOCUMENT LOG
Version Date Modified by Description of
changes
1.0 February 10 2020 Esther Serrano First release
1.4 February 2020
Pilar García (IPLA-CSIC),
Elena Ferapontova (AU),
Robert Forster (DCU),
Christine Kranz and Boris
Mizaikoff (UULM)
Added communication
activities, photos, minr
changes
FIRST ANNUAL REPORT
Grant Agreement number: 813439 — BREAK BIOFILMS — H2020-MSCA-ITN-2018
Page 2
TABLE OF CONTENT
EXECUTIVE SUMMARY ...................................................................................................... 3
INTRODUCTION – THIS IS US! .......................................................................................... 4
THE PROJECT ..................................................................................................................... 5
Why biofilms? ......................................................................................................................................... 5
How do we break biofilms? ................................................................................................................ 6
Structure of BREAK BIOFILMS .......................................................................................................... 7
THE BREAKERS .................................................................................................................. 8
OUR ACTIVITIES .............................................................................................................. 16
Scientific progress ............................................................................................................................... 16
Dissemination - Spread the Science! ............................................................................................. 17
PUBLICATIONS ................................................................................................................ 20
FOLLOW US! ..................................................................................................................... 21
GALLERY ........................................................................................................................... 22
CHESS School – 2019 (Florence) .................................................................................................... 22
BBB Meeting – 2019 (Florence) ...................................................................................................... 22
FIRST ANNUAL REPORT
Grant Agreement number: 813439 — BREAK BIOFILMS — H2020-MSCA-ITN-2018
EXECUTIVE SUMMARY
The BREAK BIOFILMS Training Network “Breaking Bad Biofilms: Innovative Analysis and
Design Rules for Next-Generation Antifouling Interfaces” started in January 2019. This report
presents the progress during the first year of the project, providing an overview of the work to
be carried out and introducing the 15 ESRs recruited.
FIRST ANNUAL REPORT
Grant Agreement number: 813439 — BREAK BIOFILMS — H2020-MSCA-ITN-2018
Page 4
INTRODUCTION – THIS IS US!
BREAK BIOFILMS is a consortium of world leaders in sensing, cell imaging, interfacial
engineering, microbiology and nanoformulation from 6 universities, 8 companies, a consortium
of food industries, a research centre, and a business and innovation centre (Table 1). A key
element of BREAK BIOFILMS is multidisciplinarity, which represents a unique strength and
opportunity to bring together 15 early stage researchers (ESRs) with diverse backgrounds.
Table 1. Participants in BREAK BIOFILMS
Short name Country
Beneficiaries
UNIVERSIDAD DE OVIEDO UNIOVI SPAIN
THE UNIVERSITY OF WARWICK WARWICK
UNITED
KINGDOM
UNIVERSITÄT ULM UULM GERMANY
UNIVERSITÀ DEGLI STUDI DI BARI ALDO
MORO UNIBA ITALY
AARHUS UNIVERSITET AU DENMARK
DUBLIN CITY UNIVERSITY DCU IRELAND
AGENCIA ESTATAL CONSEJO SUPERIOR DE
INVESTIGACIONES CIENTÍFICAS IPLA-CSIC SPAIN
ASOCIACIÓN DE INVESTIGACIÓN DE
INDUSTRIAS CÁRNICAS DEL PRINCIPADO
DE ASTURIAS
ASINCAR SPAIN
Short name Country
Partner Organisations
ARLA FOODS ARLA DENMARK
LABOR DR MERK & KOLLEGEN GMBH MERK GERMANY
TÁCTICA DESARROLLO INDUSTRIAL TÁCTICA SPAIN
PROCESS INSTRUMENTS PI UK
ABBOTT DIAGNOSTICS ABBOTT IRELAND
THERADEP TECHNOLOGIES THERADEP IRELAND
BIO-LOGIC (UNISCAN) BIOLOGIC UK
CENTRO EUROPEO DE EMPRESAS E
INNOVACIÓN DEL PRINCIPADO DE
ASTURIAS. CEEI
BIC-Asturias SPAIN
INDUSTRIAS LÁCTEAS ASTURIANAS RENY PICOT SPAIN
FIRST ANNUAL REPORT
Grant Agreement number: 813439 — BREAK BIOFILMS — H2020-MSCA-ITN-2018
THE PROJECT
Why biofilms?
Biofilms are communities of microorganisms which can be formed at all kinds of surfaces:
plastic, metals, natural materials, etc. This means that biofilms are all around us!
FIRST ANNUAL REPORT
Grant Agreement number: 813439 — BREAK BIOFILMS — H2020-MSCA-ITN-2018
Page 6
How do we break biofilms?
BREAK BIOFILMS aims to deal with the harmful impact they have in our daily lives
following an integrated strategy from biofilm detection to destruction.
These objectives are challenging and exciting. The ESR (or “the Breakers”) have access to
state-of-the-art equipment and will obtain a unique technical, industrial, and entrepreneurial
training to achieve these goals.
FIRST ANNUAL REPORT
Grant Agreement number: 813439 — BREAK BIOFILMS — H2020-MSCA-ITN-2018
Structure of BREAK BIOFILMS
This Project is structured into eight different workpackages that are interrelated and
focused on the training of the next generation leaders. Our Breakers will spread the science as
well, since they will be intimately involved in the communication activities of the network.
FIRST ANNUAL REPORT
Grant Agreement number: 813439 — BREAK BIOFILMS — H2020-MSCA-ITN-2018
Page 8
THE BREAKERS
Meet the Breakers and their projects!
Research fields: Chemistry, Chemical Engineering, Biotechnology, Nanoscience,
Pharmacy
Main supervisors: Carmen Blanco-Lo pez (cblanco@uniovi.es) and Montserrat
Rivas (rivas@uniovi.es)
Host Institution:
Research fields: Chemistry, Chemical Engineering, Biotechnology, Nanoscience,
Pharmacy
Main supervisors: Carmen Blanco-Lo pez (cblanco@uniovi.es) and Gemma
Gutie rrez (gutierrezgemma@uniovi.es)
Host Institution:
FIRST ANNUAL REPORT
Grant Agreement number: 813439 — BREAK BIOFILMS — H2020-MSCA-ITN-2018
Research fields: Chemistry, Electrochemistry, Nanoscience, Surface Chemistry
Main supervisors: Patrick Unwin (p.r.unwin@warwick.ac.uk) and Julie
Macpherson (j.macpherson@warwick.ac.uk)
Host Institution:
Research fields: Chemistry, Electrochemistry, Nanoscience, Surface Chemistry
Main supervisors: Patrick Unwin (p.r.unwin@warwick.ac.uk) and Julie
Macpherson (j.macpherson@warwick.ac.uk)
Host Institution:
FIRST ANNUAL REPORT
Grant Agreement number: 813439 — BREAK BIOFILMS — H2020-MSCA-ITN-2018
Page 10
Research fields: Analytical Chemistry, Electrochemistry, Spectroscopy,
Nanoscience, Surface Chemistry
Main supervisors: Christine Kranz (christine.kranz@uni-ulm.de) and Boris
Mizaikoff (boris.mizaikoff@uni-ulm.de)
Host Institution:
Research fields: Analytical Chemistry, Electrochemistry, Spectroscopy,
Nanoscience, Surface Chemistry
Main supervisors: Boris Mizaikoff (boris.mizaikoff@uni-ulm.de) and Christine
Kranz (christine.kranz@uni-ulm.de)
Host Institution:
FIRST ANNUAL REPORT
Grant Agreement number: 813439 — BREAK BIOFILMS — H2020-MSCA-ITN-2018
Research fields: Nanoscience, Spectroscopy, Chemistry, Surface Chemistry,
Electrochemistry, Physics, Biotechnology
Main supervisors: Nicola Cioffi (nicola.cioffi@uniba.it)
Host Institution:
Research fields: Nanoscience, Spectroscopy, Chemistry, Surface Chemistry,
Electrochemistry, Physics, Biotechnology
Main supervisors: Nicola Cioffi (nicola.cioffi@uniba.it)
Host Institution:
FIRST ANNUAL REPORT
Grant Agreement number: 813439 — BREAK BIOFILMS — H2020-MSCA-ITN-2018
Page 12
Research fields: Nanoscience, Spectroscopy, Chemistry, Surface Chemistry,
Electrochemistry, Physics, Biotechnology
Main supervisors: Elena Ferapontova (elena.ferapontova@inano.au.dk)
Host Institution:
Research fields: Chemistry, Electrochemistry, Nanoscience
Main supervisors: Elena Ferapontova (elena.ferapontova@inano.au.dk)
Host Institution:
FIRST ANNUAL REPORT
Grant Agreement number: 813439 — BREAK BIOFILMS — H2020-MSCA-ITN-2018
Research fields: Chemistry, Electrochemistry, Nanoscience, Spectroscopy
Main supervisors: Robert Forster (robert.forster@dcu.ie)
Host Institution:
Research fields: Chemistry, Electrochemistry, Nanoscience, Spectroscopy
Main supervisors: Robert forster (robert.forster@dcu.ie)
Host Institution:
FIRST ANNUAL REPORT
Grant Agreement number: 813439 — BREAK BIOFILMS — H2020-MSCA-ITN-2018
Page 14
Research fields: Biotechnology, Microbiology
Main supervisors: Pilar Garcí a (pgarcia@ipla.csic.es)
Host Institution:
Research fields: Biotechnology, Microbiology
Main supervisors: Marí a Ferna ndez (mfernandez@ipla.csic.es)
Host Institution:
FIRST ANNUAL REPORT
Grant Agreement number: 813439 — BREAK BIOFILMS — H2020-MSCA-ITN-2018
Research fields: Biology, Biotechnology, Computer Science, Chemical Engineering
Main supervisors: Juan Dí az (juan.diaz@asincar.com)
Host Institution:
FIRST ANNUAL REPORT
Grant Agreement number: 813439 — BREAK BIOFILMS — H2020-MSCA-ITN-2018
Page 16
OUR ACTIVITIES
Scientific progress
WE UNDERSTAND BIOFILMS
Develop multifunctional probes to measure simultaneously pH, reactive oxygen species or
membrane potential at bacterial colonies. Some model bacteria have been selected for
mapping topography/surface charge of samples.
Selection of antibacterial agents to deliver locally to biofilms using nanopipettes.
Investigate the swelling behaviour of AgNPs-fluoropolymers (AgCFx) composite films
with AFM, and mapping the release of Ag ions via SECM in combination with square wave
voltammetry.
WE DETECT BIOFILMS
Initial selection of signaling molecules as potential targets for biofilm detection in order
to develop a paper-based sensor.
Preparation and characterisation of gold electrodes and novel screen printed electrodes
for low cost point-of-need sensing.
Development of novel polymer based method for the immobilisation of high coverages of
active antibodies for bacteria and their characterisation using super resolution
microscopy.
WE BREAK BIOFILMS
Selection of strains for biofilms and biosensor studies.
Electrochemical production of copper-benzalkonium chloride superNAMs.
First experiments on preparation of different vesicle types for encapsulation of antibiotics
and endolysins.
Evaluation of anti-biofilm mixtures composed of endolysins and bacteriophages.
WE INHIBIT BIOFILMS
Electrodecoration of iron oxide magnetic nanoparticles by copper nanoparticles with
antibacterial activity.
First experiments to characterize the real time swelling behaviour of AgNP-fluoropolymer
composite films using atomic force microscopy.
FIRST ANNUAL REPORT
Grant Agreement number: 813439 — BREAK BIOFILMS — H2020-MSCA-ITN-2018
Dissemination - Spread the Science!
Talks and presentations about research related to BREAK BIOFILMS given by Project members
Name of the
event Location Date Title ESR, PI or partner
International Day
of Women and
Girls in Science
Oviedo,
Spain
February
11 2019 The microworld Pilar García (PI, IPLA-CSIC)
Transfer
biocompatible
materials. Official
Chamber of
Commerce of
Oviedo
Oviedo,
Spain
May 9
2019
Antimicrobials derived from
bacteriocins, bacteriophages
and endolysins
Pilar García (PI, IPLA-CSIC)
X National CyTA
Congress - CESIA León, Spain
May 16
2019
Applications of bacteriophages
in food and agriculture. Pilar García (PI, IPLA-CSIC)
Pint of Science Oviedo,
Spain
May 20
2019 Nanoparticles and biosensors
Carmen Blanco (PI,
UNIOVI)
Technological
breakfast. CSIC,
ICE, Inbiotech,
CentraTech.
León, Spain June 4,
2019
Design and evaluation of
antimicrobials Pilar García (PI, IPLA-CSIC)
Hans & Marlies
Zimmer
International
Scholar Lecture
University
of
Cincinnati,
USA
June 7,
2019
A New Look at Interfacial
Processes
Patrick Unwin (PI,
WARWICK)
PIERS Conference Rome, Italy June 17 –
20, 2019
Precision Biodiagnostics Using
Cascade Lasers Boris Mizaikoff (PI, UULM)
European Projects’
Day
Oviedo,
Spain
June 18
2019
BREAK BIOFILMS Project (oral
talk and presentation of the
poster)
Carmen Blanco (PI,
UNIOVI)
CLEO Europe
Conference
Munich,
Germany
June 23 –
27, 2019
On-Chip Infrared Sensor
Technologies for Chem/Bio
Diagnostics: Quo Vadis?
Boris Mizaikoff (PI, UULM)
Universidad de los
Andes
Bogotá,
Colombia
July 30,
2019.
Applications of bacteriophages
and endolysins in the agri-food
industry.
Pilar García (PI, IPLA-CSIC)
Workshop on
Machine Learning
and Chemometrics
in Biospectroscopy
Minsk,
Belarus
August 18
-21, 2019
Inherent Intelligence vs.
Artificial Intelligence:Infrared
Chem/Bio Sensors and
Chemometrics
Boris Mizaikoff (PI, UULM)
FIRST ANNUAL REPORT
Grant Agreement number: 813439 — BREAK BIOFILMS — H2020-MSCA-ITN-2018
Page 18
XX Euroanalysis
2019
Istanbul,
Turkey
September
2 2019
Electrochemical biosensors for
bacterial analysis (Keynote
lecture)
Elena Ferapontova (PI, AU)
RSC-TIC: Versatile
Analytical
Electrochemistry
Tokyo,
Japan
September
5 2019
Electrochemistry of Nucleic
Acids: Electrochemical Studies
and Biosensor Applications
(Tutorial lecture)
Elena Ferapontova (PI, AU)
Seminar in
Colorado State
University
Colorado
State
University,
USA
September
11, 2019
Correlative Electrochemical
Multi-Microscopy: Building an
Understanding of
Electrochemical Interfaces From
Local to Global (Hach
Distinguished Lecture)
Patrick Unwin (PI,
WARWICK)
Special Issue in a
national
newspaper (ABC)
Spain September
16 2019
Break Biofilms. NanoBioMem
research group
Carmen Blanco (PI,
UNIOVI)
European
Researchers Night
Oviedo,
Spain
September
27 2019 What is a biofilm?
Verdiana Marchianò (ESR2,
UNIOVI)
BIOCLEAN
Workshop Bari, Italy 2019
Seeing more by looking at less:
Recent advancements in
infrared biospectroscopy
Boris Mizaikoff (PI, UULM)
Winter School on
Optical Sensing
Malangen,
Norway
October
27, 2019
Waveguide-Based Infrared
Sensors Boris Mizaikoff (PI, UULM)
IX Conference on
Quality and Food
Safety 2019. Aliza
Calidad.
Oviedo,
Spain
November
7, 2019
Pathogen control in food using
bacteriophages and endolysins Pilar García (PI, IPLA-CSIC)
CHESS 2019
School
Florence,
Italy
December
6, 2019
An Overview on Scanning
(Electrochemical) Probe
Microscopies (Tutorial lecture)
Christine Kranz (PI, UULM)
and Patrick Unwin (PI,
WARWICK)
CHESS 2019
School
Florence,
Italy
December
6, 2019
Fundamentals and applications
of waveguide-based IR
spectroscopy and Sensing
Boris Mizaikoff (PI, UULM)
Warwick Sensors
Day
Warwick
University,
UK
December
11, 2019 Electrochemical Sensors
Julie Macpherson (PI,
WARWICK)
Career day
Cabra
Community
School,
Dublin,
Ireland
January
17, 2020
From Bacteria to Sensing :
Careers in Science Robert Forster (PI, DCU)
International
Conference on
Electrochemistry
in Industry Health
Mumbai,
India
January
21 – 25,
2020
Miniaturized Electrochemical
Biosensors (invited talk) Christine Kranz (PI, UULM)
FIRST ANNUAL REPORT
Grant Agreement number: 813439 — BREAK BIOFILMS — H2020-MSCA-ITN-2018
& Environment,
EIHE2020
SPIE Photonics
West
San
Francisco,
USA
February
1 – 6,
2020
Recent advancements in
surface-enhanced infrared
sensing technology: from gold
to graphene
Boris Mizaikoff (PI, UULM)
Horizon 2050:
Towards a
greener, smarter
world
Brussels,
Belgium
February
4, 2020 Sensing the future Robert Forster (PI, DCU)
International Day
of Women and
Girls in Science
Oviedo and
Avilés,
Spain
February
14, 2020 Break Biofilms
Carmen Blanco (PI,
UNIOVI)
Conferences, Workshops
Name of the
event Location Date Title of the work ESR, PI or partner
ACES 2019 Dublin,
Ireland
June 20,
2019
Advances in Anti-Microbial
Materials for Medicine Robert Forster (PI, DCU)
70th Annual
Meeting of the
International
Society of
Chemistry
Durba,
South
Africa
August 8,
2019
Modified conductive colloidal
AFM probes towards sensing at
the single entity level
Christine Kranz (PI, UULM)
CHESS 2019
School
Florence,
Italy
December
2 – 6 2019
Preliminary spectroscopic
characterization of anti-biofilm
coatings implementing ultra-
stable laser-ablated metal
nanoparticles
Ekaterina Kukushkina
(ESR8, UNIBA)
Gordon Research
Conference on
Electrochemistry
Ventura
(CA), USA
January 7,
2020
Atomistic Insights into Metal
Electrodeposition
Julie Macpherson (PI,
WARWICK)
Gordon Research
Conference on
Electrochemistry
Ventura
(CA), USA
January 9,
2020
Nano-Electrochemistry
Combined with AFM Force
Spectroscopy
Christine Kranz (PI, UULM)
NALS2020 Madrid,
Spain
January 28
– 31 2020
PLGA nanocapsules as
Superparamagnetic Iron Oxide
Nanoparticles carriers
Shayesteh Bazsefidpar
(ESR1, UNIOVI)
NALS2020 Madrid,
Spain
January 28
– 31 2020
Encapsulation of antibiotics in
vesicles
Verdiana Marchianò (ESR2,
UNIOVI)
XXII Annual Linz
Winter Workshop
on SPM
Linz,
Austria
January 31
– February
3 2020
Scanning probe microscopy
study on antimicrobial silver-
nanoparticle-based composite
films
Giada Caniglia (ESR5,
UULM)
FIRST ANNUAL REPORT
Grant Agreement number: 813439 — BREAK BIOFILMS — H2020-MSCA-ITN-2018
Page 20
PUBLICATIONS
Electrochemical Preparation of Synergistic Nanoantimicrobials. Molecules 2020, 25, 49. https://doi.org/10.3390/molecules25010049
Pros and Cons of Sacrificial Anode Electrolysis for the Preparation of Transition Metal Colloids: A Review. ChemElectroChem. doi:10.1002/celc.201901837
FIRST ANNUAL REPORT
Grant Agreement number: 813439 — BREAK BIOFILMS — H2020-MSCA-ITN-2018
Electrochemical Assays for Microbial Analysis: How Far They Are from Solving Microbiota and Microbiome Challenges, Current Opinion in Electrochemistry, 2020. 19: 153 – 161. https://doi.org/10.1016/j.coelec.2019.12.005
FOLLOW US!
Don’t miss any news or updates from the Breakers!
Find us on:
www.breakbiofilms.com
Twitter: https://twitter.com/breakbiofilms
Instagram: https://www.instagram.com/breakbiofilms/
LinkedIn: https://www.linkedin.com/company/break-biofilms/
FIRST ANNUAL REPORT
Grant Agreement number: 813439 — BREAK BIOFILMS — H2020-MSCA-ITN-2018
Page 22
GALLERY
CHESS School – 2019 (Florence)
BBB Meeting – 2019 (Florence)
FIRST ANNUAL REPORT
Grant Agreement number: 813439 — BREAK BIOFILMS — H2020-MSCA-ITN-2018
FIRST ANNUAL REPORT
Grant Agreement number: 813439 — BREAK BIOFILMS — H2020-MSCA-ITN-2018
Page 24
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