FIRST ANNUAL REPORT · Oviedo, Spain November 7, 2019 Pathogen control in food using bacteriophages and endolysins Pilar García (PI, IPLA-CSIC) CHESS 2019 School Florence, Italy

FIRST ANNUAL REPORT

28/02/2020 DELIVERABLE 7.8 – WORK PACKAGE 7

Dissemination level: Public

Ref. Ares(2020)1261111 - 28/02/2020

FIRST ANNUAL REPORT

Grant Agreement number: 813439 — BREAK BIOFILMS — H2020-MSCA-ITN-2018

FIRST ANNUAL REPORT

D E L I V E R A B L E 7 . 8 – W O R K P A C K A G E 7

DOCUMENT LOG

Version Date Modified by Description of

changes

1.0 February 10 2020 Esther Serrano First release

1.4 February 2020

Pilar García (IPLA-CSIC),

Elena Ferapontova (AU),

Robert Forster (DCU),

Christine Kranz and Boris

Mizaikoff (UULM)

Added communication

activities, photos, minr

changes

FIRST ANNUAL REPORT


Page 2

TABLE OF CONTENT

EXECUTIVE SUMMARY ...................................................................................................... 3

INTRODUCTION – THIS IS US! .......................................................................................... 4

THE PROJECT ..................................................................................................................... 5

Why biofilms? ......................................................................................................................................... 5

How do we break biofilms? ................................................................................................................ 6

Structure of BREAK BIOFILMS .......................................................................................................... 7

THE BREAKERS .................................................................................................................. 8

OUR ACTIVITIES .............................................................................................................. 16

Scientific progress ............................................................................................................................... 16

Dissemination - Spread the Science! ............................................................................................. 17

PUBLICATIONS ................................................................................................................ 20

FOLLOW US! ..................................................................................................................... 21

GALLERY ........................................................................................................................... 22

CHESS School – 2019 (Florence) .................................................................................................... 22

BBB Meeting – 2019 (Florence) ...................................................................................................... 22

FIRST ANNUAL REPORT


EXECUTIVE SUMMARY

The BREAK BIOFILMS Training Network “Breaking Bad Biofilms: Innovative Analysis and

Design Rules for Next-Generation Antifouling Interfaces” started in January 2019. This report

presents the progress during the first year of the project, providing an overview of the work to

be carried out and introducing the 15 ESRs recruited.

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INTRODUCTION – THIS IS US!

BREAK BIOFILMS is a consortium of world leaders in sensing, cell imaging, interfacial

engineering, microbiology and nanoformulation from 6 universities, 8 companies, a consortium

of food industries, a research centre, and a business and innovation centre (Table 1). A key

element of BREAK BIOFILMS is multidisciplinarity, which represents a unique strength and

opportunity to bring together 15 early stage researchers (ESRs) with diverse backgrounds.

Table 1. Participants in BREAK BIOFILMS

Short name Country

Beneficiaries

UNIVERSIDAD DE OVIEDO UNIOVI SPAIN

THE UNIVERSITY OF WARWICK WARWICK

UNITED

KINGDOM

UNIVERSITÄT ULM UULM GERMANY

UNIVERSITÀ DEGLI STUDI DI BARI ALDO

MORO UNIBA ITALY

AARHUS UNIVERSITET AU DENMARK

DUBLIN CITY UNIVERSITY DCU IRELAND

AGENCIA ESTATAL CONSEJO SUPERIOR DE

INVESTIGACIONES CIENTÍFICAS IPLA-CSIC SPAIN

ASOCIACIÓN DE INVESTIGACIÓN DE

INDUSTRIAS CÁRNICAS DEL PRINCIPADO

DE ASTURIAS

ASINCAR SPAIN

Short name Country

Partner Organisations

ARLA FOODS ARLA DENMARK

LABOR DR MERK & KOLLEGEN GMBH MERK GERMANY

TÁCTICA DESARROLLO INDUSTRIAL TÁCTICA SPAIN

PROCESS INSTRUMENTS PI UK

ABBOTT DIAGNOSTICS ABBOTT IRELAND

THERADEP TECHNOLOGIES THERADEP IRELAND

BIO-LOGIC (UNISCAN) BIOLOGIC UK

CENTRO EUROPEO DE EMPRESAS E

INNOVACIÓN DEL PRINCIPADO DE

ASTURIAS. CEEI

BIC-Asturias SPAIN

INDUSTRIAS LÁCTEAS ASTURIANAS RENY PICOT SPAIN

FIRST ANNUAL REPORT


THE PROJECT

Why biofilms?

Biofilms are communities of microorganisms which can be formed at all kinds of surfaces:

plastic, metals, natural materials, etc. This means that biofilms are all around us!

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How do we break biofilms?

BREAK BIOFILMS aims to deal with the harmful impact they have in our daily lives

following an integrated strategy from biofilm detection to destruction.

These objectives are challenging and exciting. The ESR (or “the Breakers”) have access to

state-of-the-art equipment and will obtain a unique technical, industrial, and entrepreneurial

training to achieve these goals.

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Structure of BREAK BIOFILMS

This Project is structured into eight different workpackages that are interrelated and

focused on the training of the next generation leaders. Our Breakers will spread the science as

well, since they will be intimately involved in the communication activities of the network.

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THE BREAKERS

Meet the Breakers and their projects!

Research fields: Chemistry, Chemical Engineering, Biotechnology, Nanoscience,

Pharmacy

Main supervisors: Carmen Blanco-Lo pez ([email protected]) and Montserrat

Rivas ([email protected])

Host Institution:

Research fields: Chemistry, Chemical Engineering, Biotechnology, Nanoscience,

Pharmacy

Main supervisors: Carmen Blanco-Lo pez ([email protected]) and Gemma

Gutie rrez ([email protected])

Host Institution:

mailto:[email protected]




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Research fields: Chemistry, Electrochemistry, Nanoscience, Surface Chemistry

Main supervisors: Patrick Unwin ([email protected]) and Julie

Macpherson ([email protected])

Host Institution:

Research fields: Chemistry, Electrochemistry, Nanoscience, Surface Chemistry

Main supervisors: Patrick Unwin ([email protected]) and Julie

Macpherson ([email protected])

Host Institution:





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Research fields: Analytical Chemistry, Electrochemistry, Spectroscopy,

Nanoscience, Surface Chemistry

Main supervisors: Christine Kranz ([email protected]) and Boris

Mizaikoff ([email protected])

Host Institution:

Research fields: Analytical Chemistry, Electrochemistry, Spectroscopy,

Nanoscience, Surface Chemistry

Main supervisors: Boris Mizaikoff ([email protected]) and Christine

Kranz ([email protected])

Host Institution:





FIRST ANNUAL REPORT


Research fields: Nanoscience, Spectroscopy, Chemistry, Surface Chemistry,

Electrochemistry, Physics, Biotechnology

Main supervisors: Nicola Cioffi ([email protected])

Host Institution:



Main supervisors: Nicola Cioffi ([email protected])

Host Institution:


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Main supervisors: Elena Ferapontova ([email protected])

Host Institution:

Research fields: Chemistry, Electrochemistry, Nanoscience

Main supervisors: Elena Ferapontova ([email protected])

Host Institution:



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Research fields: Chemistry, Electrochemistry, Nanoscience, Spectroscopy

Main supervisors: Robert Forster ([email protected])

Host Institution:

Research fields: Chemistry, Electrochemistry, Nanoscience, Spectroscopy

Main supervisors: Robert forster ([email protected])

Host Institution:



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Research fields: Biotechnology, Microbiology

Main supervisors: Pilar Garcí a ([email protected])

Host Institution:

Research fields: Biotechnology, Microbiology

Main supervisors: Marí a Ferna ndez ([email protected])

Host Institution:



FIRST ANNUAL REPORT


Research fields: Biology, Biotechnology, Computer Science, Chemical Engineering

Main supervisors: Juan Dí az ([email protected])

Host Institution:


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OUR ACTIVITIES

Scientific progress

WE UNDERSTAND BIOFILMS

Develop multifunctional probes to measure simultaneously pH, reactive oxygen species or

membrane potential at bacterial colonies. Some model bacteria have been selected for

mapping topography/surface charge of samples.

Selection of antibacterial agents to deliver locally to biofilms using nanopipettes.

Investigate the swelling behaviour of AgNPs-fluoropolymers (AgCFx) composite films

with AFM, and mapping the release of Ag ions via SECM in combination with square wave

voltammetry.

WE DETECT BIOFILMS

Initial selection of signaling molecules as potential targets for biofilm detection in order

to develop a paper-based sensor.

Preparation and characterisation of gold electrodes and novel screen printed electrodes

for low cost point-of-need sensing.

Development of novel polymer based method for the immobilisation of high coverages of

active antibodies for bacteria and their characterisation using super resolution

microscopy.

WE BREAK BIOFILMS

Selection of strains for biofilms and biosensor studies.

Electrochemical production of copper-benzalkonium chloride superNAMs.

First experiments on preparation of different vesicle types for encapsulation of antibiotics

and endolysins.

Evaluation of anti-biofilm mixtures composed of endolysins and bacteriophages.

WE INHIBIT BIOFILMS

Electrodecoration of iron oxide magnetic nanoparticles by copper nanoparticles with

antibacterial activity.

First experiments to characterize the real time swelling behaviour of AgNP-fluoropolymer

composite films using atomic force microscopy.

FIRST ANNUAL REPORT


Dissemination - Spread the Science!

Talks and presentations about research related to BREAK BIOFILMS given by Project members

Name of the

event Location Date Title ESR, PI or partner

International Day

of Women and

Girls in Science

Oviedo,

Spain

February

11 2019 The microworld Pilar García (PI, IPLA-CSIC)

Transfer

biocompatible

materials. Official

Chamber of

Commerce of

Oviedo

Oviedo,

Spain

May 9

2019

Antimicrobials derived from

bacteriocins, bacteriophages

and endolysins

Pilar García (PI, IPLA-CSIC)

X National CyTA

Congress - CESIA León, Spain

May 16

2019

Applications of bacteriophages

in food and agriculture. Pilar García (PI, IPLA-CSIC)

Pint of Science Oviedo,

Spain

May 20

2019 Nanoparticles and biosensors

Carmen Blanco (PI,

UNIOVI)

Technological

breakfast. CSIC,

ICE, Inbiotech,

CentraTech.

León, Spain June 4,

2019

Design and evaluation of

antimicrobials Pilar García (PI, IPLA-CSIC)

Hans & Marlies

Zimmer

International

Scholar Lecture

University

of

Cincinnati,

USA

June 7,

2019

A New Look at Interfacial

Processes

Patrick Unwin (PI,

WARWICK)

PIERS Conference Rome, Italy June 17 –

20, 2019

Precision Biodiagnostics Using

Cascade Lasers Boris Mizaikoff (PI, UULM)

European Projects’

Day

Oviedo,

Spain

June 18

2019

BREAK BIOFILMS Project (oral

talk and presentation of the

poster)

Carmen Blanco (PI,

UNIOVI)

CLEO Europe

Conference

Munich,

Germany

June 23 –

27, 2019

On-Chip Infrared Sensor

Technologies for Chem/Bio

Diagnostics: Quo Vadis?

Boris Mizaikoff (PI, UULM)

Universidad de los

Andes

Bogotá,

Colombia

July 30,

2019.

Applications of bacteriophages

and endolysins in the agri-food

industry.

Pilar García (PI, IPLA-CSIC)

Workshop on

Machine Learning

and Chemometrics

in Biospectroscopy

Minsk,

Belarus

August 18

-21, 2019

Inherent Intelligence vs.

Artificial Intelligence:Infrared

Chem/Bio Sensors and

Chemometrics


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XX Euroanalysis

2019

Istanbul,

Turkey

September

2 2019

Electrochemical biosensors for

bacterial analysis (Keynote

lecture)

Elena Ferapontova (PI, AU)

RSC-TIC: Versatile

Analytical

Electrochemistry

Tokyo,

Japan

September

5 2019

Electrochemistry of Nucleic

Acids: Electrochemical Studies

and Biosensor Applications

(Tutorial lecture)

Elena Ferapontova (PI, AU)

Seminar in

Colorado State

University

Colorado

State

University,

USA

September

11, 2019

Correlative Electrochemical

Multi-Microscopy: Building an

Understanding of

Electrochemical Interfaces From

Local to Global (Hach

Distinguished Lecture)

Patrick Unwin (PI,

WARWICK)

Special Issue in a

national

newspaper (ABC)

Spain September

16 2019

Break Biofilms. NanoBioMem

research group

Carmen Blanco (PI,

UNIOVI)

European

Researchers Night

Oviedo,

Spain

September

27 2019 What is a biofilm?

Verdiana Marchianò (ESR2,

UNIOVI)

BIOCLEAN

Workshop Bari, Italy 2019

Seeing more by looking at less:

Recent advancements in

infrared biospectroscopy


Winter School on

Optical Sensing

Malangen,

Norway

October

27, 2019

Waveguide-Based Infrared

Sensors Boris Mizaikoff (PI, UULM)

IX Conference on

Quality and Food

Safety 2019. Aliza

Calidad.

Oviedo,

Spain

November

7, 2019

Pathogen control in food using

bacteriophages and endolysins Pilar García (PI, IPLA-CSIC)

CHESS 2019

School

Florence,

Italy

December

6, 2019

An Overview on Scanning

(Electrochemical) Probe

Microscopies (Tutorial lecture)

Christine Kranz (PI, UULM)

and Patrick Unwin (PI,

WARWICK)

CHESS 2019

School

Florence,

Italy

December

6, 2019

Fundamentals and applications

of waveguide-based IR

spectroscopy and Sensing


Warwick Sensors

Day

Warwick

University,

UK

December

11, 2019 Electrochemical Sensors

Julie Macpherson (PI,

WARWICK)

Career day

Cabra

Community

School,

Dublin,

Ireland

January

17, 2020

From Bacteria to Sensing :

Careers in Science Robert Forster (PI, DCU)

International

Conference on

Electrochemistry

in Industry Health

Mumbai,

India

January

21 – 25,

2020

Miniaturized Electrochemical

Biosensors (invited talk) Christine Kranz (PI, UULM)

FIRST ANNUAL REPORT


& Environment,

EIHE2020

SPIE Photonics

West

San

Francisco,

USA

February

1 – 6,

2020

Recent advancements in

surface-enhanced infrared

sensing technology: from gold

to graphene


Horizon 2050:

Towards a

greener, smarter

world

Brussels,

Belgium

February

4, 2020 Sensing the future Robert Forster (PI, DCU)

International Day

of Women and

Girls in Science

Oviedo and

Avilés,

Spain

February

14, 2020 Break Biofilms

Carmen Blanco (PI,

UNIOVI)

Conferences, Workshops

Name of the

event Location Date Title of the work ESR, PI or partner

ACES 2019 Dublin,

Ireland

June 20,

2019

Advances in Anti-Microbial

Materials for Medicine Robert Forster (PI, DCU)

70th Annual

Meeting of the

International

Society of

Chemistry

Durba,

South

Africa

August 8,

2019

Modified conductive colloidal

AFM probes towards sensing at

the single entity level


CHESS 2019

School

Florence,

Italy

December

2 – 6 2019

Preliminary spectroscopic

characterization of anti-biofilm

coatings implementing ultra-

stable laser-ablated metal

nanoparticles

Ekaterina Kukushkina

(ESR8, UNIBA)

Gordon Research

Conference on

Electrochemistry

Ventura

(CA), USA

January 7,

2020

Atomistic Insights into Metal

Electrodeposition

Julie Macpherson (PI,

WARWICK)

Gordon Research

Conference on

Electrochemistry

Ventura

(CA), USA

January 9,

2020

Nano-Electrochemistry

Combined with AFM Force

Spectroscopy


NALS2020 Madrid,

Spain

January 28

– 31 2020

PLGA nanocapsules as

Superparamagnetic Iron Oxide

Nanoparticles carriers

Shayesteh Bazsefidpar

(ESR1, UNIOVI)

NALS2020 Madrid,

Spain

January 28

– 31 2020

Encapsulation of antibiotics in

vesicles

Verdiana Marchianò (ESR2,

UNIOVI)

XXII Annual Linz

Winter Workshop

on SPM

Linz,

Austria

January 31

– February

3 2020

Scanning probe microscopy

study on antimicrobial silver-

nanoparticle-based composite

films

Giada Caniglia (ESR5,

UULM)

FIRST ANNUAL REPORT


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PUBLICATIONS

Electrochemical Preparation of Synergistic Nanoantimicrobials. Molecules 2020, 25, 49. https://doi.org/10.3390/molecules25010049

Pros and Cons of Sacrificial Anode Electrolysis for the Preparation of Transition Metal Colloids: A Review. ChemElectroChem. doi:10.1002/celc.201901837

https://doi.org/10.3390/molecules25010049

https://onlinelibrary.wiley.com/doi/abs/10.1002/celc.201901837

FIRST ANNUAL REPORT


Electrochemical Assays for Microbial Analysis: How Far They Are from Solving Microbiota and Microbiome Challenges, Current Opinion in Electrochemistry, 2020. 19: 153 – 161. https://doi.org/10.1016/j.coelec.2019.12.005

FOLLOW US!

Don’t miss any news or updates from the Breakers!

Find us on:

www.breakbiofilms.com

Twitter: https://twitter.com/breakbiofilms

Instagram: https://www.instagram.com/breakbiofilms/

LinkedIn: https://www.linkedin.com/company/break-biofilms/

https://doi.org/10.1016/j.coelec.2019.12.005

http://www.breakbiofilms.com/

https://twitter.com/breakbiofilms

https://www.instagram.com/breakbiofilms/

https://www.linkedin.com/company/break-biofilms/

FIRST ANNUAL REPORT


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GALLERY

CHESS School – 2019 (Florence)

BBB Meeting – 2019 (Florence)

FIRST ANNUAL REPORT


FIRST ANNUAL REPORT


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Documents

FIRST ANNUAL REPORT · Oviedo, Spain November 7, 2019 Pathogen control in food using bacteriophages and endolysins Pilar García (PI, IPLA-CSIC) CHESS 2019 School Florence, Italy