brucelle by franchesca grepo

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THE BRUCELLAEFlorencondia, Jean Loren O.Grepo, Franchesca D.BSP3D

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Identificationand

morphologyPathogensis Diagnostic

laboratory testPharmacologic

interventionPran

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▪Brucellosis, also known as “undulant“Mediterranean fever” or “Malta fevzoonosis and the infection is

invariably transmitted by direct or icontact with infected animals oproducts – (WHO)

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Important specie

Zoonosis

Br. Melitensis- Goat

Br. Abortus- abortion in Cattle

Br. ovis- sheep

Br. suis- pigs

Br. Canis - dogs

B. neotomae desert wood rat

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Identification and Morphology

Gram negative coccobacilli

Intracellular Parasite

Strict aerobic

Non motile

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  Non spore

1.2 microm

Colonies ap

in 2-5 days

Small, convcolonies

Identification and Morphology

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Transmission

Skin TransmissionAccidental penetration and abrasion: Farmers and Veterinarians

Respiratory transmission

Laboratory workers

Oral transmissionunpasteurised milk & products, raw milk or meet.

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Pathogenesis

Enters the body

Macrophage activationPolymorph migration & Phagocy

Intracellular multiplication

Res organs

blood

Any organ

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Diagnostic laboratory test A. Specimens

Bloodlymph nodes, bonefor Culture

Serumserologic test

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Diagnostic laboratory test B. Culture

Brucella Agar

Trypticase soy agar with orwithout 5% sheep blood

Brain-heart infusion medium

Chocolate agar

All cultures should be incubated in 8-10% Carbon dioxide at 35-37 degrees. Ob

urea

• positive

oxida

• posit

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Diagnostic laboratory test C. Serology

• IgG agglutinin titers above 1:80 indicateactive infection.

• If negative, presence of “blockingantibodies” 

Agglutinationtest

• IgA antibodies that interfere with agglutination by IgGand IgM, cause a serologic test to be negative.Blocking

antibodies

• IgG, IgA & IgM may be detected.

• Use of cytoplasmic protein as antigen

• More sensitive and specific than agglutination testELISA Assays

Ig

• 1st wk. of acpeak at 3mopersist durinillness.

Ig

• 3 weeks after ondisease, peak atremain high durdisease

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ELISA and Serologic test

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Culturing brucella

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Treatment

▪ Susceptible to tetracyclines, rifampin, trimethoprsulfamethoxazole, aminoglycoside and some quin

▪ Combined treatment with tetracycline and estreptomycin for 2-3 weeks or rifampin for 6

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Prevention and Control

Pasteurizationof milk or milk

products

reduction ofoccupational

hazards

In laboratorymust have a

strict biosafetyprecautions

Cdda

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References

WHO.INT &&

Jawetz, Melnick & Adelberg’s 

MEDICAL MICROBIOLOGY 26th e

Submitted to:

Mr. Bryan de PaduaMicrobiology & Parasitology