8/12/2019 brucelle by franchesca grepo

http://slidepdf.com/reader/full/brucelle-by-franchesca-grepo 1/17

THE BRUCELLAEFlorencondia, Jean Loren O.Grepo, Franchesca D.BSP3D

8/12/2019 brucelle by franchesca grepo

http://slidepdf.com/reader/full/brucelle-by-franchesca-grepo 2/17

 

Identificationand

morphologyPathogensis Diagnostic

laboratory testPharmacologic

interventionPran

8/12/2019 brucelle by franchesca grepo

http://slidepdf.com/reader/full/brucelle-by-franchesca-grepo 3/17

▪Brucellosis, also known as “undulant“Mediterranean fever” or “Malta fevzoonosis and the infection is

invariably transmitted by direct or icontact with infected animals oproducts – (WHO)

8/12/2019 brucelle by franchesca grepo

http://slidepdf.com/reader/full/brucelle-by-franchesca-grepo 4/17

Important specie

Zoonosis

Br. Melitensis- Goat

Br. Abortus- abortion in Cattle

Br. ovis- sheep

Br. suis- pigs

Br. Canis - dogs

B. neotomae desert wood rat

8/12/2019 brucelle by franchesca grepo

http://slidepdf.com/reader/full/brucelle-by-franchesca-grepo 5/17

Identification and Morphology

Gram negative coccobacilli

Intracellular Parasite

Strict aerobic

Non motile

8/12/2019 brucelle by franchesca grepo

http://slidepdf.com/reader/full/brucelle-by-franchesca-grepo 6/17

  Non spore

1.2 microm

Colonies ap

in 2-5 days

Small, convcolonies

Identification and Morphology

8/12/2019 brucelle by franchesca grepo

http://slidepdf.com/reader/full/brucelle-by-franchesca-grepo 7/17

Transmission

Skin TransmissionAccidental penetration and abrasion: Farmers and Veterinarians

Respiratory transmission

Laboratory workers

Oral transmissionunpasteurised milk & products, raw milk or meet.

8/12/2019 brucelle by franchesca grepo

http://slidepdf.com/reader/full/brucelle-by-franchesca-grepo 8/17

Pathogenesis

Enters the body

Macrophage activationPolymorph migration & Phagocy

Intracellular multiplication

Res organs

blood

Any organ

8/12/2019 brucelle by franchesca grepo

http://slidepdf.com/reader/full/brucelle-by-franchesca-grepo 9/17

8/12/2019 brucelle by franchesca grepo

http://slidepdf.com/reader/full/brucelle-by-franchesca-grepo 10/17

Diagnostic laboratory test A. Specimens

Bloodlymph nodes, bonefor Culture

Serumserologic test

8/12/2019 brucelle by franchesca grepo

http://slidepdf.com/reader/full/brucelle-by-franchesca-grepo 11/17

Diagnostic laboratory test B. Culture

Brucella Agar

Trypticase soy agar with orwithout 5% sheep blood

Brain-heart infusion medium

Chocolate agar

All cultures should be incubated in 8-10% Carbon dioxide at 35-37 degrees. Ob

urea

• positive

oxida

• posit

8/12/2019 brucelle by franchesca grepo

http://slidepdf.com/reader/full/brucelle-by-franchesca-grepo 12/17

Diagnostic laboratory test C. Serology

• IgG agglutinin titers above 1:80 indicateactive infection.

• If negative, presence of “blockingantibodies” 

Agglutinationtest

• IgA antibodies that interfere with agglutination by IgGand IgM, cause a serologic test to be negative.Blocking

antibodies

• IgG, IgA & IgM may be detected.

• Use of cytoplasmic protein as antigen

• More sensitive and specific than agglutination testELISA Assays

Ig

• 1st wk. of acpeak at 3mopersist durinillness.

Ig

• 3 weeks after ondisease, peak atremain high durdisease

8/12/2019 brucelle by franchesca grepo

http://slidepdf.com/reader/full/brucelle-by-franchesca-grepo 13/17

ELISA and Serologic test

8/12/2019 brucelle by franchesca grepo

http://slidepdf.com/reader/full/brucelle-by-franchesca-grepo 14/17

Culturing brucella

8/12/2019 brucelle by franchesca grepo

http://slidepdf.com/reader/full/brucelle-by-franchesca-grepo 15/17

Treatment

▪ Susceptible to tetracyclines, rifampin, trimethoprsulfamethoxazole, aminoglycoside and some quin

▪ Combined treatment with tetracycline and estreptomycin for 2-3 weeks or rifampin for 6

8/12/2019 brucelle by franchesca grepo

http://slidepdf.com/reader/full/brucelle-by-franchesca-grepo 16/17

Prevention and Control

Pasteurizationof milk or milk

products

reduction ofoccupational

hazards

In laboratorymust have a

strict biosafetyprecautions

Cdda

8/12/2019 brucelle by franchesca grepo

http://slidepdf.com/reader/full/brucelle-by-franchesca-grepo 17/17

References

WHO.INT &&

Jawetz, Melnick & Adelberg’s 

MEDICAL MICROBIOLOGY 26th e

Submitted to:

Mr. Bryan de PaduaMicrobiology & Parasitology