Automated Extraction System Method Note Compendium€¦ · Compendium Updated September 25, 2019....

EDGE®

Automated Extraction System

Method Note Compendium

Updated September 25, 2019

Agriculture

Extraction of Pesticides From Avocados 2

Extraction of Polyphenols From Beans 3

Extraction of Polyphenols From Cacao 4

Extraction of Cannabinoids From Cannabis Plant 5

Extraction of Mycotoxins From Cannabis Plant 6

Extraction of Pesticides From Cannabis Plant 7

Extraction of Total Fat From Feeds/Foods 8

Extraction of PCBs From Feeds/Foods (Wet) 9

Extraction of Dioxins From Feeds/Foods (Wet) 10

Extraction of PCBs From Fish Meal (Dry) 11

Extraction of PAHs From Fish Tissue (Dry) 12

Extraction of PAHs From Fish Tissue (Wet) 13

Extraction of Pesticides From Foods (Wet) 14

Extraction of Pesticides From Hops 15

Extraction of Fat From Nuts (Brazil) 16

Extraction of Fat From Nuts (Hazelnuts) 17

Extraction of Fat From Pumpkin Seeds 18

Extraction of Brodifacoum From Rat Poison Powder 19

Extraction of Pesticides From Rice 20

Extraction of PCBs From Soy Meal (Dry) 21

Extraction of Pesticides From Spices (Dry) 22

Extraction of Pesticides From Strawberries 23

Cannabis

Extraction of THC/THCA From Cannabis Flower 25

Extraction of Cannabinoids From Cannabis Plant 26

Extraction of Mycotoxins From Cannabis Plant 27

Extraction of Pesticides From Cannabis Plant 28

ContentsEnvironmental & Regulatory

Extraction of SVOCs Environmental Samples 30

Extraction of SVOCs EPA 3545A 31

Extraction of Dioxins From Calcined Kiln Dust 32

Extraction of SVOCs, Pesticides, TPH, PCB From Cement Mix 33

Extraction of SVOCs From Environmental Samples 34

Extraction of PAHs From Fish Tissue (Wet) 35

Extraction of Organics From Lake Sediment 36

Extraction of PCBs From PUF Filters (Large) 37

Extraction of PCBs From PUF Filters (Small) 38

Extraction of PAHs From Quartz Air Filter 39

Extraction of Dioxins From Soil 40

Extraction of Diesel and Oils From Soil 41

Extraction of PAHs From Soil, Loam or Clay 42

Extraction of PCBs From Soil, Loam or Clay 43

Extraction of Dioxins From Soil, Loam or Clay 44

Cleaning of PUF Filters 45

Food Testing

Extraction of Pesticides From Avocados 47

Extraction of Polyphenols From Beans 48

Extraction of Fat From Bone Meal 49

Extraction of Fat From Bread 50

Extraction of Polyphenols From Cacao 51

Extraction of Cannabinoids From Cannabis Plant 52

Extraction of Mycotoxins From Cannabis Plant 53

Extraction of Pesticides From Cannabis Plant 54

Extraction of Lipids From Chicken (Baked) 55

Extraction of Lipids From Chicken (Fried) 56

Hydrolysis & Extraction of Total Fat From Chocolate 57

Hydrolysis & Extraction of Total Fat From Cookies 58

Extraction of Fat From DATEM Powder 59

Extraction of Flavonoids from Echinacea 60

Extraction of Fat From Egg Powder 61

Extraction of Total Fat From Feeds/Foods 62

Extraction of PCBs From Feeds/Foods (Wet) 63

Extraction of Dioxins From Feeds/Foods (Wet) 64

Extraction of PCBs From Fish Meal (Dry) 65

Extraction of PAHs From Fish Tissue (Dry) 66

Extraction of PAHs From Fish Tissue (Wet) 67

Extraction of Pesticides From Foods (Wet) 68

Extraction From Goldenseal Root 69

Extraction of Pesticides From Hops 70

Extraction of Total Fat From Hot Dogs 71

Extraction of PAHs From Infant Formula 72

Extraction of Total Fat From Meats (High-Fat) 73

Extraction of Total Fat From Meats (Potted) 74

Extraction of Fat From Nuts (Brazil) 75

Extraction of Fat From Nuts (Cashews) 76

Extraction of Fat From Nuts (Hazelnuts) 77

Extraction of Total Fat From Nuts (Peanuts) 78

Extraction of Total Fat From Pepperoni 79

Extraction of Lipids From Potatoes (Baked) 80

Extraction of Lipids From Potatoes (Fried) 81

Extraction of Nutrients From Protein Powder 82

Extraction of Total Fat From Protein Powder 83

Extraction of Fat From Pumpkin Seeds 84

Extraction of Pesticides From Rice 85

Extraction of PCBs From Soy Meal (Dry) 86

Extraction of Pesticides From Spices (Dry) 87

Extraction From Spirulina Algae 88

Extraction of Pesticides From Strawberries 89

Extraction of PAHs From Yerba Mate 90

Geoscience & Mining

Extraction of Dioxins From Calcined Kiln Dust 92

Extraction of SVOCs, Pesticides, TPH, PCB From Cement Mix 93

Extraction of Molybdenum 94

Industrial Hygiene

Extraction of PCBs From PUF Filters (Large) 96

Extraction of PCBs From PUF Filters (Small) 97

Extraction of Brodifacoum From Rat Poison Powder 98

Cleaning of PUF Filters 99

Materials Science

Extraction of Film Powder 101

Extraction of Pigments From Plastics 102

Extraction of Additives From Polypropylene Powder 103

Extraction of Phthalates From Polyvinyl Chloride 104

Extraction of PCBs From PUF Filters (Large) 105

Extraction of PCBs From PUF Filters (Small) 106

Extraction of Brodifacoum From Rat Poison Powder 107

Extraction of Antioxidants From Resin 108

Extraction of PAHs From XAD Resin 109

Cleaning of PUF Filters 110

Metals & Alloys

Extraction of Molybdenum 112

Nutraceuticals

Extraction of Flavonoids From Echinacea 114

Extraction of PAHs From Fish Tissue (Dry) 115

Extraction of PAHs From Fish Tissue (Wet) 116

Extraction From Goldenseal Root 117

Extraction of PAHs From Infant Formula 118

Extraction of Nutrients From Protein Powder 119

Extraction of Total Fat From Protein Powder 120

Extraction From Spirulina Algae 121

Extraction of PAHs From Yerba Mate 122

Personal Care Products

Extraction of PAHs From Infant Formula 124

Extraction of Mouthwash 125

Extraction of Toothpaste 126

Plastics, Polymers, & Oils

Extraction of Film Powder 128

Extraction of Additives From PET 129

Extraction of Additives From Pigment 130

Extraction of Pigments From Plastics 131

Extraction of Additives From Polyethylene Powder 132

Extraction of Chemical Modifier From Polyethylene Resin 133

Extraction of Additives From Polypropylene Powder 134

Extraction of Phthalates From Polyvinyl Chloride 135

Extraction of Diesel and Oils From Soil 136

Agriculture

1

Extraction of PesticidesFrom AvocadosFrom Avocados

Agriculture, Food TestingEDGE

Note: Temperature and hold times may vary depending on the sample and analytes of interest.

Heating Program

Sample Weight5 g

Q-DiscM2 + C9

EquipmentEDGE40 mL collection vials - clear

NotesNone

Procedure1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc®. Layer the M2 Q-Disc (textured side up) on top of the C9 Q-Disc. 2. Weigh the sorbent into the Q-Cup. Spread evenly across the base of the Q-Cup by gentle tapping.3. Weigh the sample into the Q-Cup layering on top of the sorbent. No mixing is required.

Solvents1% Acetic Acid in Acetonitrile

SorbentsQ-Matrix Hydraᵀᴹ (2.5 g)C18 (2 g)

Wash Program

Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)

1 4020 1:00010

Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)

1 1% Acetic Acid in Acetonitrile 15 0:1540

2 1% Acetic Acid in Acetonitrile 15 0:0040

General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.

label2

Extraction of PolyphenolsFrom BeansFrom Beans

Agriculture, Food TestingEDGE

Note: Temperature and hold times may vary depending on the sample and analytes of interest.

Heating Program

Sample Weight0.1 g

Q-DiscC1

EquipmentEDGE40 mL collection vials - clear

NotesNone

Procedure 1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc®. 2. Weigh the sample into the Q-Cup.

Solvents(1:1) Methanol/Water

SorbentsN/A

Wash Program

Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)

1 8020 10:001010

Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)

1 (1:1) Methanol/Water 15 0:1580

2 (1:1) Methanol/Water 15 0:0080

General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.

label3

Extraction of PolyphenolsFrom CacaoFrom Cacao

Agriculture, Food TestingEDGE

Note: Temperature and hold times may vary depending on the sample and analytes of interest.

Heating Program

Sample Weight0.1 g

Q-DiscC1

EquipmentEDGE40 mL collection vials - clear

NotesNone

Procedure 1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc®. 2. Weigh the sample into the Q-Cup.

Solvents(1:1) Methanol/Water

SorbentsN/A

Wash Program

Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)

1 8020 10:001010

Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)

1 (1:1) Methanol/Water 15 0:1580

2 (1:1) Methanol/Water 15 0:0080

General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.

label4

Extraction of CannabinoidsFrom Cannabis PlantFrom Cannabis Plant

Agriculture, Cannabis, Food TestingEDGE

Note: Temperature and hold times may vary depending on the sample and analytes of interest.

Heating Program

Sample Weight1 g

Q-DiscC9 + G1 + C9

EquipmentEDGE40 mL collection vials - clear

NotesPossessing, using, distributing, or selling marijuana or marijuana-based products constitute federal crimes in the United States, even where a state law decriminalizes or legalizes such activities. CEM Corporation produces instruments that are intended for use in testing laboratories and applications only where such use is permitted under applicable state/country law.

Procedure1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc®. Sandwich the G1 disc between two C9 discs. 2. Weigh the sample into the Q-Cup.

SolventsOptions:MethanolIsopropanol

SorbentsN/A

Wash Program

Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)

1 9010 0:3055

Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)

1 Methanol 30 0:3090

2 Isopropanol 20 0:3090

General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.

label5

Extraction of MycotoxinsFrom Cannabis PlantFrom Cannabis Plant

Agriculture, Cannabis, Food TestingEDGE

Note: Temperature and hold times may vary depending on the sample and analytes of interest.

Heating Program

Sample Weight1.5 g

Q-DiscM2 + C9C4

EquipmentEDGE40 mL collection vials - clear

NotesPossessing, using, distributing, or selling marijuana or marijuana-based products constitute federal crimes in the United States, even where a state law decriminalizes or legalizes such activities. CEM Corporation produces instruments that are intended for use in testing laboratories and applications only where such use is permitted under applicable state/country law.Either C4 or M2/C9 Q-Disc can be used.

Procedure 1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc. Layr the M2 disc (textured sided up) on top of the C9

disc, if M2 used. 2. Weigh the sample into the Q-Cup.

SolventsOptions:Methanol May also use a percentage of Water/Methanol as needed

SorbentsN/A

Wash Program

Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)

1 5015 2:0055

Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)

1 Methanol 15 0:3050

2 Methanol 15 0:3050

General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.

label6

Extraction of PesticidesFrom Cannabis PlantFrom Cannabis Plant

Agriculture, Cannabis, Food TestingEDGE

Note: Temperature and hold times may vary depending on the sample and analytes of interest.

Heating Program

Sample Weight1.5 g

Q-DiscM2 + C9

EquipmentEDGE40 mL collection vials - clear

NotesPossessing, using, distributing, or selling marijuana or marijuana-based products constitute federal crimes in the United States, even where a state law decriminalizes or legalizes such activities. CEM Corporation produces instruments that are intended for use in testing laboratories and applications only where such use is permitted under applicable state/country law.

Procedure1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc®. Layer the M2 Q-Disc (textured side up) on top of the C9 Q-Disc.2. Weigh the sorbent into the Q-Cup. Spread evenly across the base of the Q-Cup by gentle tapping.3. Weigh the sample into the Q-Cup layering the sample on top of the sorbent. No mixing is required.

Solvents1% Acetic Acid in Acetonitrile

SorbentsContents of Agilent QuEChERS SPE 15 mL Tube

Wash Program

Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)

1 4020 2:0055

Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)

1 1% Acetic Acid in Acetonitrile 15 0:3040

2 1% Acetic Acid in Acetonitrile 15 0:3040

General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.

label7

Extraction of Total FatFrom Feeds/FoodsFrom Feeds/Foods

Agriculture, Food TestingEDGE

Note: Temperature and hold times may vary depending on the sample and analytes of interest.

Heating Program

Sample Weight≤ 5 g

Q-DiscC1

EquipmentEDGE40 mL collection vials - clear

NotesNone

Procedure1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc®. 2. Weigh the sand into the Q-Cup. Spread evenly across the base of the Q-Cup by gentle tapping.3. Weigh the sample into the Q-Cup. Gently mix the sample and sand in the Q-Cup.4. Dry Q-Cups with sample and sand in the oven for 1 hour at 100° C while tilted at an angle in ~2" aluminum pans or equivalent. This provides stability and enables easier visual identification that no sample has been lost.

SolventsPetroleum Ether

SorbentsWet Samples Only: Sand (use equal amount to sample size)

Wash Program

Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)

1 14010 1:00010

2 14010 1:00010

Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)

1 Petroleum Ether 15 0:15150

2 Petroleum Ether 15 0:00150

General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.

label8

Extraction of PCBsFrom Feeds/Foods (Wet)From Feeds/Foods (Wet)

Agriculture, Food TestingEDGE

Note: Temperature and hold times may vary depending on the sample and analytes of interest.

Heating Program

Sample Weight5 g

Q-DiscC9 + G1 + C9

EquipmentEDGE40 mL collection vials - clear

NotesNone

Procedure1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc®. Sandwich the G1 disc between the two C9 discs.2. Weigh the sorbent into the Q-Cup. Spread evenly across the base of the Q-Cup by gentle tapping.3. Weigh the sample into the Q-Cup layering on top of the sorbent. No mixing is needed.

SolventsDichloromethane

SorbentsC18 (2.5 g)

Wash Program

Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)

1 13520 2:00510

Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)

1 Dichloromethane 15 0:15150

2 Dichloromethane 15 0:00150

General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.

label9

Extraction of DioxinsFrom Feeds/Foods (Wet)From Feeds/Foods (Wet)

Agriculture, Food TestingEDGE

Note: Temperature and hold times may vary depending on the sample and analytes of interest.

Heating Program

Sample Weight5 g

Q-DiscC4

EquipmentEDGE40 mL collection vials - clear

NotesNone

Procedure1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc®. 2. Weigh the sorbent into the Q-Cup. Spread evenly across the base of the Q-Cup by gentle tapping.3. Weigh the sample into the Q-Cup layering on top of the sorbent. No mixing is needed.

SolventsDichloromethane

SorbentsC18 (2 g)

Wash Program

Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)

1 15020 2:00510

Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)

1 Dichloromethane 15 0:15150

2 Dichloromethane 15 0:00150

General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.

label10

Extraction of PCBsFrom Fish Meal (Dry)From Fish Meal (Dry)

Agriculture, Food TestingEDGE

Note: Temperature and hold times may vary depending on the sample and analytes of interest.

Heating Program

Sample Weight5 g

Q-DiscC1M1 + C9

EquipmentEDGE40 mL collection vials - clear

NotesLess than 5 grams of sample can be used with a ratio of 1:2 C18: Sample.Either C1 or M1/C9 Q-Disc can be used.

Procedure1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc®. If using the M1 disc, layer a C9 disc on top of M1.2. Weigh the sorbent into the Q-Cup. Spread evenly across the base of the Q-Cup by gentle tapping.3. Weigh the sample into the Q-Cup layering the sample on top of the sorbent. No mixing is required.

SolventsOptions:Dichloromethane(1:1) Hexane/Acetone

SorbentsC18 (2.5 g)

Wash Program

Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)

1 12020 3:001010

Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)

1 Dichloromethane 15 0:15150

2 Extraction Solvent 10 0:00150

General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.

label11

Extraction of PAHsFrom Fish Tissue (Dry)From Fish Tissue (Dry)

Agriculture, Food Testing, NutraceuticalEDGE

Note: Temperature and hold times may vary depending on the sample and analytes of interest.

Heating Program

Sample Weight5 g

Q-DiscC1

EquipmentEDGE40 mL collection vials - clear

NotesNone

Procedure1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc®.2. Weigh the sorbent in the Q-Cup. Tap lightly to spread evenly.3. Weigh the sample into the Q-Cup layering the sample on top of the sorbent. No mixing is required.

SolventsDichloromethane

SorbentsC18 (1.7 g)

Wash Program

Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)

1 12020 3:001010

Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)

1 Dichloromethane 15 0:30150

2 Dichloromethane 15 0:00150

General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.

label12

Extraction of PAHsFrom Fish Tissue (Wet)From Fish Tissue (Wet)

Agriculture, Environmental and RegulatEDGE

Note: Temperature and hold times may vary depending on the sample and analytes of interest.

Heating Program

Sample Weight2 g

Q-DiscC9 + G1 + C9

EquipmentEDGE40 mL collection vials - clear

NotesIf there is moisture in collection vial after the extraction:Using a glass funnel with glass wool and about 20 g of Sodium Sulfate. Pour over into new collection vial and rinse thoroughly with fresh solvent.

Procedure1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc®. Sandwich the G1 disc between the two C9 discs.2. Weigh the sorbent into the Q-Cup. Spread evenly across the base of the Q-Cup by gentle tapping.3. Weigh the sample into the Q-Cup layering on top of the sorbent. No mixing is needed.

SolventsOptions:Dichloromethane(1:1) Hexane/Acetone

SorbentsC18 (0.8 g)

Wash Program

Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)

1 12020 3:001010

Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)

1 Extraction Solvent 15 0:15150

2 Extraction Solvent 15 0:00150

General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.

label13

Extraction of PesticidesFrom Foods (Wet)From Foods (Wet)

Agriculture, Food TestingEDGE

Note: Temperature and hold times may vary depending on the sample and analytes of interest.

Heating Program

Sample Weight5 g

Q-DiscC9 + G1 + C9

EquipmentEDGE40 mL collection vials - clear

NotesNone

Procedure1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc®. Sandwich the G1 disc between two C9 discs.2. Weigh the sorbents into the Q-Cup. Spread evenly across the sample by gentle tapping.3. Weigh the sample into the Q-Cup layering the sample on top of the sorbents. No mixing is required.

Solvents1% Acetic Acid in Acetonitrile

SorbentsQ-Matrix Hydraᵀᴹ (2.5 g)

Wash Program

Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)

1 4020 3:00010

Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)

1 1% Acetic Acid in Acetonitrile 15 0:1540

2 1% Acetic Acid in Acetonitrile 15 0:0040

General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.

label14

Extraction of PesticidesFrom HopsFrom Hops

Agriculture, Food TestingEDGE

Note: Temperature and hold times may vary depending on the sample and analytes of interest.

Heating Program

Sample Weight0.5 g

Q-DiscM2 + C9

EquipmentEDGE40 mL collection vials - clear

NotesCanadian Pesticide Mix 4 in LCMS grade obtained from SPEX CertiPrep.

Procedure1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc®. Layer the M2 Q-Disc (textured side up) on top of the C9 Q-Disc.2. Add sorbents to bottom of Q-Cup. Spread evenly across the base of the Q-Cup by gentle tapping. No mixing is required.3. Weigh the sample into the Q-Cup layering on top of the sorbent. No mixing is required.4. Spike with 100 ug/mL Canadian Pesticide Mix 4 and leave in hood for approximately 30 minutes to evaporate solvent.

Solvents1% Acetic Acid in Acetonitrile

SorbentsQ-Matrix Hydraᵀᴹ (1.25 g)Supelᵀᴹ QuE Citrate (1.25 g)Primary secondary amine (1.25 g)Siliabond C18 (1.25 g)*Different sorbents can be used

Wash Program

Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)

1 4015 3:00510

Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)

1 1% Acetic Acid in Acetonitrile 15 0:0040

2 1% Acetic Acid in Acetonitrile 15 0:0040

General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.

label15

Extraction of FatFrom Nuts (Brazil)From Nuts (Brazil)

Agriculture, Food TestingEDGE

Note: Temperature and hold times may vary depending on the sample and analytes of interest.

Heating Program

Sample Weight12 g

Q-DiscC1

EquipmentEDGE40 mL collection vials - clear

NotesNone

Procedure1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc®. 2. Weigh the sand into the Q-Cup. Spread evenly across the base of the Q-Cup by gentle tapping.3. Weigh the sample into the Q-Cup. Gently mix the sample and the sand in the Q-Cup.

SolventsPetroleum Ether

SorbentsSand (12 g)

Wash Program

Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)

1 14020 1:0000

2 14015 1:0005

Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)

1 Petroleum Ether 15 0:15150

2 Petroleum Ether 15 0:00150

General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.

label16

Extraction of FatFrom Nuts (Hazelnuts)From Nuts (Hazelnuts)

Agriculture, Food TestingEDGE

Note: Temperature and hold times may vary depending on the sample and analytes of interest.

Heating Program

Sample Weight12 g

Q-DiscC1

EquipmentEDGE40 mL collection vials - clear

NotesNone

Procedure1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc®. 2. Weigh the sand into the Q-Cup. Spread evenly across the base of the Q-Cup by gentle tapping.3. Weigh the sample into the Q-Cup. Gently mix the sample and sand in the Q-Cup.

SolventsPetroleum Ether

SorbentsSand (12 g)

Wash Program

Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)

1 14020 1:0000

2 14015 1:0005

Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)

1 Petroleum Ether 15 0:15150

2 Petroleum Ether 0 0:00150

General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.

label17

Extraction of FatFrom Pumpkin SeedsFrom Pumpkin Seeds

Agriculture, Food TestingEDGE

Note: Temperature and hold times may vary depending on the sample and analytes of interest.

Heating Program

Sample Weight12 g

Q-DiscC1

EquipmentEDGE40 mL collection vials - clear

NotesNone

Procedure1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc®. 2. Weigh the sand into the Q-Cup. Spread evenly across the base of the Q-Cup by gentle tapping.3. Weigh the sample into the Q-Cup. Gently mix the sample and sand in the Q-Cup.

SolventsPetroleum Ether

SorbentsSand (12 g)

Wash Program

Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)

1 14020 1:0000

2 14015 1:0005

Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)

1 Petroleum Ether 15 0:15150

2 Petroleum Ether 15 0:00150

General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.

label18

Extraction of BrodifacoumFrom Rat Poison PowderFrom Rat Poison Powder

Agriculture, Industrial Hygiene, MaterialEDGE

Note: Temperature and hold times may vary depending on the sample and analytes of interest.

Heating Program

Sample Weight2 g

Q-DiscC4

EquipmentEDGE40 mL collection vials - clear

NotesNone

Procedure 1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc®. 2. Weigh the sample into the Q-Cup.

SolventsMethanol

SorbentsN/A

Wash Program

Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)

1 13015 2:001010

2 13015 2:001010

Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)

1 Methanol 15 0:15150

2 Methanol 15 0:00150

General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.

label19

Extraction of PesticidesFrom RiceFrom Rice

Agriculture, Food TestingEDGE

Note: Temperature and hold times may vary depending on the sample and analytes of interest.

Heating Program

Sample Weight5 g

Q-DiscM2 + C9

EquipmentEDGE40 mL collection vials - clear

NotesNone

Procedure1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc®. Layer the M2 Q-Disc (textured side up) on top of the C9 Q-Disc. 2. Weigh the sorbents into the Q-Cup. Spread evenly across the sample by gentle tapping.3. Weigh the sample into the Q-Cup layering the sample on top of the sorbent. No mixing is required.

Solvents1% Acetic Acid in Acetonitile

SorbentsQ-Matrix Hydraᵀᴹ (2.5 g)

Wash Program

Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)

1 4020 3:00010

Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)

1 1% Acetic Acid in Acetonitrile 15 0:1540

2 1% Acetic Acid in Acetonitrile 15 0:0040

General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.

label20

Extraction of PCBsFrom Soy Meal (Dry)From Soy Meal (Dry)

Agriculture, Food TestingEDGE

Note: Temperature and hold times may vary depending on the sample and analytes of interest.

Heating Program

Sample Weight5 g

Q-DiscC1M1 + C9

EquipmentEDGE40 mL collection vials - clear

NotesLess than 5 grams of sample can be used with a ratio of 1:2 C18/Sample.Either C1 or M1/C9 Q-Disc can be used.

Procedure1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc®. If using M1 disc, layer on top of C9 disc.2. Weigh the sorbent into the Q-Cup. Spread evenly across the base of the Q-Cup by gentle tapping.3. Weigh the sample into the Q-Cup layering the sample on top of the sorbent. No mixing is required.

SolventsOptions:Dichloromethane(1:1) Hexane/Acetone

SorbentsC18 (2.5 g)

Wash Program

Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)

1 12020 3:001010

Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)

1 Dichloromethane 15 0:15150

2 Extraction Solvent 10 0:00150

General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.

label21

Extraction of PesticidesFrom Spices (Dry)From Spices (Dry)

Agriculture, Food TestingEDGE

Note: Temperature and hold times may vary depending on the sample and analytes of interest.

Heating Program

Sample Weight1 g

Q-DiscC9 + G1 + C9

EquipmentEDGE40 mL collection vials - clear

NotesMatrix matched calibration curve required without the use of sorbents.

Procedure1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc®. Sandwich the G1 disc between the two C9 discs.2. Weigh the sample into the Q-Cup.

Solvents1% Acetic Acid in Acetonitrile

SorbentsN/A

Wash Program

Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)

1 4015 3:00510

Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)

1 1% Acetic Acid in Acetonitrile 15 0:1040

2 1% Acetic Acid in Acetonitrile 15 0:0040

General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.

label22

Extraction of PesticidesFrom StrawberriesFrom Strawberries

Agriculture, Food TestingEDGE

Note: Temperature and hold times may vary depending on the sample and analytes of interest.

Heating Program

Sample Weight5 g

Q-DiscC9 + G1 + C9

EquipmentEDGE40 mL collection vials - clear

NotesNone

Procedure1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc®. Sandwich the G1 disc between 2 C9 Q-Disc. 2. Weigh the sorbents into the Q-Cup. Spread evenly across the sample by gentle tapping.3. Weigh the sample into the Q-Cup layering the sample on top of the sorbent. No mixing is required.

Solvents1% Acetic Acid in Acetonitrile

SorbentsQ-Matrix Hydraᵀᴹ (2.5 g)Primary Second Amine (2 g)

Wash Program

Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)

1 4020 3:00010

Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)

1 1% Acetic Acid in Acetonitrile 15 0:1040

2 1% Acetic Acid in Acetonitrile 15 0:0040

General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.

label23

Cannabis

24

Extraction of THC/THCAFrom Cannabis PlantFrom Cannabis Plant

CannabisEDGE

Note: Temperature and hold times may vary depending on the sample and analytes of interest.

Heating Program

Sample Weight1 g

Q-DiscC9 + G1 + C9

EquipmentEDGE40 mL collection vials - clear

NotesPossessing, using, distributing, or selling marijuana or marijuana-based products constitute federal crimes in the United States, even where a state law decriminalizes or legalizes such activities. CEM Corporation produces instruments that are intended for use in testing laboratories and applications only where such use is permitted under applicable state/country law.

Procedure1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup®. Sandwich the G1 disc between the two C9 discs.2. Weigh the sample into the Q-Cup.

SolventsOptions:MethanolIsopropanol

SorbentsN/A

Wash Program

Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)

1 9010 0:3055

Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)

1 Methanol 30 0:3090

2 Isopropanol 20 0:3090

General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.

label25

Extraction of CannabinoidsFrom Cannabis PlantFrom Cannabis Plant

Agriculture, Cannabis, Food TestingEDGE

Note: Temperature and hold times may vary depending on the sample and analytes of interest.

Heating Program

Sample Weight1 g

Q-DiscC9 + G1 + C9

EquipmentEDGE40 mL collection vials - clear

NotesPossessing, using, distributing, or selling marijuana or marijuana-based products constitute federal crimes in the United States, even where a state law decriminalizes or legalizes such activities. CEM Corporation produces instruments that are intended for use in testing laboratories and applications only where such use is permitted under applicable state/country law.

Procedure1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc®. Sandwich the G1 disc between two C9 discs. 2. Weigh the sample into the Q-Cup.

SolventsOptions:MethanolIsopropanol

SorbentsN/A

Wash Program

Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)

1 9010 0:3055

Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)

1 Methanol 30 0:3090

2 Isopropanol 20 0:3090

General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.

label26

Extraction of MycotoxinsFrom Cannabis PlantFrom Cannabis Plant

Agriculture, Cannabis, Food TestingEDGE

Note: Temperature and hold times may vary depending on the sample and analytes of interest.

Heating Program

Sample Weight1.5 g

Q-DiscM2 + C9C4

EquipmentEDGE40 mL collection vials - clear

NotesPossessing, using, distributing, or selling marijuana or marijuana-based products constitute federal crimes in the United States, even where a state law decriminalizes or legalizes such activities. CEM Corporation produces instruments that are intended for use in testing laboratories and applications only where such use is permitted under applicable state/country law.Either C4 or M2/C9 Q-Disc can be used.

Procedure 1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc. Layr the M2 disc (textured sided up) on top of the C9

disc, if M2 used. 2. Weigh the sample into the Q-Cup.

SolventsOptions:Methanol May also use a percentage of Water/Methanol as needed

SorbentsN/A

Wash Program

Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)

1 5015 2:0055

Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)

1 Methanol 15 0:3050

2 Methanol 15 0:3050

General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.

label27

Extraction of PesticidesFrom Cannabis PlantFrom Cannabis Plant

Agriculture, Cannabis, Food TestingEDGE

Note: Temperature and hold times may vary depending on the sample and analytes of interest.

Heating Program

Sample Weight1.5 g

Q-DiscM2 + C9

EquipmentEDGE40 mL collection vials - clear

NotesPossessing, using, distributing, or selling marijuana or marijuana-based products constitute federal crimes in the United States, even where a state law decriminalizes or legalizes such activities. CEM Corporation produces instruments that are intended for use in testing laboratories and applications only where such use is permitted under applicable state/country law.

Procedure1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc®. Layer the M2 Q-Disc (textured side up) on top of the C9 Q-Disc.2. Weigh the sorbent into the Q-Cup. Spread evenly across the base of the Q-Cup by gentle tapping.3. Weigh the sample into the Q-Cup layering the sample on top of the sorbent. No mixing is required.

Solvents1% Acetic Acid in Acetonitrile

SorbentsContents of Agilent QuEChERS SPE 15 mL Tube

Wash Program

Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)

1 4020 2:0055

Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)

1 1% Acetic Acid in Acetonitrile 15 0:3040

2 1% Acetic Acid in Acetonitrile 15 0:3040

General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.

label28

Environmental & Regulatory

29

Extraction of SVOCsEnvironmental SamplesEnvironmental Samples

Environmental and RegulatoryEDGE

Note: Temperature and hold times may vary depending on the sample and analytes of interest.

Heating Program

Sample Weight30 g

Q-DiscC4

EquipmentEDGE40 mL collection vials - clear

NotesThis is a general method for the miscellaneous extraction of environmental samples.

Procedure 1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc®. 2. Weigh the sample into the Q-Cup.

SolventsOptions:AcetoneDichloromethaneHexaneToluene

SorbentsN/A

Wash Program

Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)

1 12015 2:001010

Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)

1 Dichloromethane 20 0:15150

2 Extraction Solvent 10 0:00150

General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.

label30

Extraction of SVOCsEPA 3545AEPA 3545A

Environmental and RegulatoryEDGE

Note: Temperature and hold times may vary depending on the sample and analytes of interest.

Heating Program

Sample Weight30 g

Q-DiscC4

EquipmentEDGE40 mL collection vials - clear

NotesSee the CEM Application note: Extraction of Semi-Volatile Organic Compounds from Soil in Accordance with EPA 3545A if needed.

Procedure 1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc®. 2. Weigh the sample into the Q-Cup.

Solvents(1:1) Acetone/Hexane

SorbentsN/A

Wash Program

Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)

1 12015 2:001010

Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)

1 (1:1) Acetone/Hexane 30 0:15150

2 (1:1) Acetone/Hexane 30 0:00150

General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.

label31

Extraction of DioxinsFrom Calcined Kiln DustFrom Calcined Kiln Dust

Environmental and Regulatory, GeoscieEDGE

Note: Temperature and hold times may vary depending on the sample and analytes of interest.

Heating Program

Sample Weight10 g

Q-DiscC9 + G1 + C9

EquipmentEDGE60 mL collection vials - amber

NotesNone

Procedure1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup®. Sandwich the G1 Q-Disc® between the two C9 Q-Discs. 2. Weigh the sample into the Q-Cup.

SolventsOptions:DichloromethaneMethyl tert-butyl ether

SorbentsN/A

Wash Program

Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)

1 15020 2:0000

2 15020 2:0000

3 15020 2:0000

Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)

1 Dichloromethane 20 0:30150

2 Methyl tert-butyl ether 20 0:30150

General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.

label32

Extraction of SVOCs, Pesticides, TPH, PCBFrom Cement MixFrom Cement Mix

Environmental and Regulatory, GeoscieEDGE

Note: Temperature and hold times may vary depending on the sample and analytes of interest.

Heating Program

Sample Weight15 g

Q-DiscC9 + G1 + C9

EquipmentEDGE40 mL collection vials - clear

NotesNone

Procedure1. Assemble the Q-Cup® with the Q-Disc®. Sandwich the G1 disc between two C9 discs. 2. Weigh the sample into the Q-Cup.

Solvents(1:1) Acetone/Dichloromethane

SorbentsN/A

Wash Program

Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)

1 10020 3:001010

Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)

1 (1:1) Acetone/Dichloromethane 15 0:30150

2 (1:1) Acetone/Dichloromethane 15 0:00150

General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.

label33

Extraction of SVOCsFrom Environmental SamplesFrom Environmental Samples

Environmental and RegulatoryEDGE

Note: Temperature and hold times may vary depending on the sample and analytes of interest.

Heating Program

Sample Weight30 g

Q-DiscC4

EquipmentEDGE40 mL collection vials - clear

NotesThis is a general method for the miscellaneous extraction of environmental samples.

Procedure1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc®.2. Weigh the sample into the Q-Cup.

SolventsOptions:Acetone DichloromethaneHexaneToluene

SorbentsN/A

Wash Program

Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)

1 12015 2:001010

Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)

1 Dichloromethane 20 0:15150

2 Extraction Solvent 10 0:00150

General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.

label34

Extraction of PAHsFrom Fish Tissue (Wet)From Fish Tissue (Wet)

Agriculture, Environmental and RegulatEDGE

Note: Temperature and hold times may vary depending on the sample and analytes of interest.

Heating Program

Sample Weight2 g

Q-DiscC9 + G1 + C9

EquipmentEDGE40 mL collection vials - clear

NotesIf there is moisture in collection vial after the extraction:Using a glass funnel with glass wool and about 20 g of Sodium Sulfate. Pour over into new collection vial and rinse thoroughly with fresh solvent.

Procedure1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc®. Sandwich the G1 disc between the two C9 discs.2. Weigh the sorbent into the Q-Cup. Spread evenly across the base of the Q-Cup by gentle tapping.3. Weigh the sample into the Q-Cup layering on top of the sorbent. No mixing is needed.

SolventsOptions:Dichloromethane(1:1) Hexane/Acetone

SorbentsC18 (0.8 g)

Wash Program

Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)

1 12020 3:001010

Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)

1 Extraction Solvent 15 0:15150

2 Extraction Solvent 15 0:00150

General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.

label35

Extraction of OrganicsFrom Lake SedimentFrom Lake Sediment

Environmental and Regulatory, Organic EDGE

Note: Temperature and hold times may vary depending on the sample and analytes of interest.

Heating Program

Sample Weight1 g

Q-DiscC1

EquipmentEDGE40 mL collection vials - clear

NotesNone

Procedure1. Homogenize, grind, or cryomill sample. Assemble the Q-Cup® with the Q-Disc®.2. Weigh the sample into the Q-Cup.

Solvents(1:1) Methanol/Dichloromethane

SorbentsN/A

Wash Program

Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)

1 10020 2:0000

2 10020 2:0000

Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)

1 (1:1) Methanol/Dichloromethane 15 0:20150

General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.

label36

Extraction of PCBsFrom PUF Filters (Large)From PUF Filters (Large)

Environmental and Regulatory, IndustriaEDGE

Note: Temperature and hold times may vary depending on the sample and analytes of interest.

Heating Program

Sample WeightEntire Filter

Q-DiscC1

EquipmentEDGE40 mL collection vials - clear

NotesNone

Procedure 1. Assemble the Q-Cup® with the Q-Disc®. 2. Compress and push the filter into the base of the Q-Cup.

SolventsHexane

SorbentsN/A

Wash Program

Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)

1 11520 3:00155

Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)

1 Hexane 10 0:15150

2 Hexane 10 0:00150

General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.

label37

Extraction of PCBsFrom PUF Filters (Small)From PUF Filters (Small)

Environmental and Regulatory, IndustriaEDGE

Note: Temperature and hold times may vary depending on the sample and analytes of interest.

Heating Program

Sample WeightEntire Filter

Q-DiscC1

EquipmentEDGE40 mL collection vials - clear

NotesNone

Procedure1. Assemble the Q-Cup® with the Q-Disc®. 2. Compress and push the filter into the base of the Q-Cup.

SolventsHexane

SorbentsN/A

Wash Program

Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)

1 10015 0:10100

Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)

1 Hexane 10 0:15150

2 Hexane 10 0:00150

General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.

label38

Extraction of PAHsFrom Quartz Air FilterFrom Quartz Air Filter

Environmental and RegulatoryEDGE

Note: Temperature and hold times may vary depending on the sample and analytes of interest.

Heating Program

Sample Weight3 g

Q-DiscC9 + G1 + C9

EquipmentEDGE40 mL collection vials - clear

NotesNone

Procedure1. Assemble the Q-Cup® with the Q-Disc®. 2. Weigh the sorbent in the Q-Cup. Tap lightly to spread evenly.3. Add the sample on top of the sorbent. No mixing is required.

Solvents(1:1) Hexane/Dichloromethane

SorbentsSand (3 g)

Wash Program

Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)

1 12020 2:00010

Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)

1 (1:1) Hexane/Dichloromethane 15 0:15100

2 (1:1) Hexane/Dichloromethane 15 0:00100

General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.

label39

Extraction of DioxinsFrom SoilFrom Soil

Environmental and RegulatoryEDGE

Note: Temperature and hold times may vary depending on the sample and analytes of interest.

Heating Program

Sample Weight10 g

Q-DiscC1

EquipmentEDGE60 mL collection vials - clear

NotesNone

Procedure 1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc®. 2. Weigh the sample into the Q-Cup.

SolventsOptions:DichloromethaneMethy tert-butyl etherToluene

SorbentsN/A

Wash Program

Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)

1 15020 1:0000

2 15020 1:0000

3 15020 1:0000

Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)

1 Extraction Solvent 30 0:30150

2 Extraction Solvent 30 0:30150

General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.

label40

Extraction of Diesel and OilsFrom SoilFrom Soil

Environmental and Regulatory, Plastics,EDGE

Note: Temperature and hold times may vary depending on the sample and analytes of interest.

Heating Program

Sample Weight5 g

Q-DiscC9 + G1 + C9

EquipmentEDGE40 mL collection vials - clear

NotesNone

Procedure1. Assemble the Q-Cup® with the Q-Disc®. Sandwich the G1 disc between the two C9 discs. 2. Weigh the sorbent into the Q-Cup.3. Weigh the sample into the Q-Cup on top of the sorbent. No mixing is required.

SolventsDichloromethane

SorbentsDiatomaceous Earth (1 g)

Wash Program

Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)

1 10020 2:0000

2 10010 2:00010

Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)

1 Dichloromethane 15 0:30150

2 Dichloromethane 15 0:00150

General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.

label41

Extraction of PAHsFrom Soil, Loam or ClayFrom Soil, Loam or Clay

Environmental and RegulatoryEDGE

Note: Temperature and hold times may vary depending on the sample and analytes of interest.

Heating Program

Sample Weight30 g

Q-DiscC1

EquipmentEDGE40 mL collection vials - clear

NotesNone

Procedure 1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc®. 2. Weigh the sample into the Q-Cup.

Solvents(1:1) Acetone/Hexane

SorbentsN/A

Wash Program

Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)

1 12015 1:001010

Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)

1 (1:1) Acetone/Hexane 15 0:30150

2 (1:1) Acetone/Hexane 15 0:00150

General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.

label42

Extraction of PCBsFrom Soil, Loam or ClayFrom Soil, Loam or Clay

Environmental and RegulatoryEDGE

Note: Temperature and hold times may vary depending on the sample and analytes of interest.

Heating Program

Sample Weight30 g

Q-DiscC4

EquipmentEDGE40 mL collection vials - clear

NotesNone

Procedure1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc®. 2. Weigh the sample into the Q-Cup.

Solvents(1:1) Acetone/Hexane

SorbentsN/A

Wash Program

Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)

1 12015 1:001010

Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)

1 (1:1) Acetone/Hexane 15 0:30150

2 (1:1) Acetone/Hexane 15 0:00150

General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.d) Bring to volume or evaporate as necessary for analysis.

label43

Extraction of DioxinsFrom Soil, Loam or ClayFrom Soil, Loam or Clay

Environmental and RegulatoryEDGE

Note: Temperature and hold times may vary depending on the sample and analytes of interest.

Heating Program

Sample Weight2 g

Q-DiscC1

EquipmentEDGE60 mL collection vials - clear

NotesNone

Procedure 1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc®. 2. Weigh the sample into the Q-Cup.

SolventsToluene

SorbentsN/A

Wash Program

Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)

1 15020 1:0000

2 15020 1:0000

3 15015 1:0005

Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)

1 Toluene 30 0:30150

2 Toluene 30 0:30150

General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.

label44

Cleaning ofPUF FiltersPUF Filters

Environmental and Regulatory, IndustriaEDGE

Note: Temperature and hold times may vary depending on the sample and analytes of interest.

Heating Program

Sample WeightEntire Filter

Q-DiscC1

EquipmentEDGE40 mL collection vials - clear

NotesNone

Procedure 1. Assemble the Q-Cup® with the Q-Disc®. 2. Compress and push the filter into the base of the Q-Cup.

SolventsOptions:HexaneAcetone

SorbentsN/A

Wash Program

Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)

1 11510 3:00100

2 11510 3:00100

3 11530 3:00100

Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)

1 Hexane 15 0:15150

2 Acetone 15 0:00150

General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.

label45

Food Testing

46

Extraction of PesticidesFrom AvocadosFrom Avocados

Agriculture, Food TestingEDGE

Note: Temperature and hold times may vary depending on the sample and analytes of interest.

Heating Program

Sample Weight5 g

Q-DiscM2 + C9

EquipmentEDGE40 mL collection vials - clear

NotesNone

Procedure1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc®. Layer the M2 Q-Disc (textured side up) on top of the C9 Q-Disc. 2. Weigh the sorbent into the Q-Cup. Spread evenly across the base of the Q-Cup by gentle tapping.3. Weigh the sample into the Q-Cup layering on top of the sorbent. No mixing is required.

Solvents1% Acetic Acid in Acetonitrile

SorbentsQ-Matrix Hydraᵀᴹ (2.5 g)C18 (2 g)

Wash Program

Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)

1 4020 1:00010

Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)

1 1% Acetic Acid in Acetonitrile 15 0:1540

2 1% Acetic Acid in Acetonitrile 15 0:0040

General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.

label47

Extraction of PolyphenolsFrom BeansFrom Beans

Agriculture, Food TestingEDGE

Note: Temperature and hold times may vary depending on the sample and analytes of interest.

Heating Program

Sample Weight0.1 g

Q-DiscC1

EquipmentEDGE40 mL collection vials - clear

NotesNone

Procedure 1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc®. 2. Weigh the sample into the Q-Cup.

Solvents(1:1) Methanol/Water

SorbentsN/A

Wash Program

Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)

1 8020 10:001010

Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)

1 (1:1) Methanol/Water 15 0:1580

2 (1:1) Methanol/Water 15 0:0080

General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtainrequired results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position andload method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.

label48

Extraction of FatFrom Bone MealFrom Bone Meal

Food TestingEDGE

Note: Temperature and hold times may vary depending on the sample and analytes of interest.

Heating Program

Sample Weight10 g

Q-DiscC4

EquipmentEDGE40 mL collection vials - clear

NotesNone

Procedure1. Assemble the Q-Cup® with the Q-Disc®.2. Weigh the sorbent into the Q-Cup.3. Weigh the sample into the Q-Cup. Gently mix the sample and the sorbent in the Q-Cup.

SolventsPetroleum Ether

SorbentsDiatomaceous Earth (5 g)

Wash Program

Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)

1 14020 1:0000

2 14010 1:00010

Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)

1 Petroluem Ether 15 0:15150

2 Petroluem Ether 15 0:00150

General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.

label49

Extraction of FatFrom BreadFrom Bread

Food TestingEDGE

Note: Temperature and hold times may vary depending on the sample and analytes of interest.

Heating Program

Sample Weight4 g

Q-DiscC1

EquipmentEDGE40 mL collection vials - clear

NotesNone

Procedure1. Assemble the Q-Cup® with the Q-Disc®.2. Dry the sample and mill, or blend, to a fine powder.3. Weigh the sample into the Q-Cup.

SolventsPetroleum Ether

SorbentsN/A

Wash Program

Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)

1 14010 1:00010

2 14010 1:00010

Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)

1 Petroleum Ether 15 0:15150

2 Petroleum Ether 15 0:00150

General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.

label50

Extraction of PolyphenolsFrom CacaoFrom Cacao

Agriculture, Food TestingEDGE

Note: Temperature and hold times may vary depending on the sample and analytes of interest.

Heating Program

Sample Weight0.1 g

Q-DiscC1

EquipmentEDGE40 mL collection vials - clear

NotesNone

Procedure 1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc®. 2. Weigh the sample into the Q-Cup.

Solvents(1:1) Methanol/Water

SorbentsN/A

Wash Program

Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)

1 8020 10:001010

Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)

1 (1:1) Methanol/Water 15 0:1580

2 (1:1) Methanol/Water 15 0:0080

General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.

label51

Extraction of CannabinoidsFrom Cannabis PlantFrom Cannabis Plant

Agriculture, Cannabis, Food TestingEDGE

Note: Temperature and hold times may vary depending on the sample and analytes of interest.

Heating Program

Sample Weight1 g

Q-DiscC9 + G1 + C9

EquipmentEDGE40 mL collection vials - clear

NotesPossessing, using, distributing, or selling marijuana or marijuana-based products constitute federal crimes in the United States, even where a state law decriminalizes or legalizes such activities. CEM Corporation produces instruments that are intended for use in testing laboratories and applications only where such use is permitted under applicable state/country law.

Procedure1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc®. Sandwich the G1 disc between two C9 discs. 2. Weigh the sample into the Q-Cup.

SolventsOptions:MethanolIsopropanol

SorbentsN/A

Wash Program

Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)

1 9010 0:3055

Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)

1 Methanol 30 0:3090

2 Isopropanol 20 0:3090

General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.

label52

Extraction of MycotoxinsFrom Cannabis PlantFrom Cannabis Plant

Agriculture, Cannabis, Food TestingEDGE

Note: Temperature and hold times may vary depending on the sample and analytes of interest.

Heating Program

Sample Weight1.5 g

Q-DiscM2 + C9C4

EquipmentEDGE40 mL collection vials - clear

NotesPossessing, using, distributing, or selling marijuana or marijuana-based products constitute federal crimes in the United States, even where a state law decriminalizes or legalizes such activities. CEM Corporation produces instruments that are intended for use in testing laboratories and applications only where such use is permitted under applicable state/country law.Either C4 or M2/C9 Q-Disc can be used.

Procedure 1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc. Layr the M2 disc (textured sided up) on top of the C9

disc, if M2 used. 2. Weigh the sample into the Q-Cup.

SolventsOptions:Methanol May also use a percentage of Water/Methanol as needed

SorbentsN/A

Wash Program

Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)

1 5015 2:0055

Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)

1 Methanol 15 0:3050

2 Methanol 15 0:3050

General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.

label53

Extraction of PesticidesFrom Cannabis PlantFrom Cannabis Plant

Agriculture, Cannabis, Food TestingEDGE

Note: Temperature and hold times may vary depending on the sample and analytes of interest.

Heating Program

Sample Weight1.5 g

Q-DiscM2 + C9

EquipmentEDGE40 mL collection vials - clear

NotesPossessing, using, distributing, or selling marijuana or marijuana-based products constitute federal crimes in the United States, even where a state law decriminalizes or legalizes such activities. CEM Corporation produces instruments that are intended for use in testing laboratories and applications only where such use is permitted under applicable state/country law.

Procedure1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc®. Layer the M2 Q-Disc (textured side up) on top of the C9 Q-Disc.2. Weigh the sorbent into the Q-Cup. Spread evenly across the base of the Q-Cup by gentle tapping.3. Weigh the sample into the Q-Cup layering the sample on top of the sorbent. No mixing is required.

Solvents1% Acetic Acid in Acetonitrile

SorbentsContents of Agilent QuEChERS SPE 15 mL Tube

Wash Program

Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)

1 4020 2:0055

Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)

1 1% Acetic Acid in Acetonitrile 15 0:3040

2 1% Acetic Acid in Acetonitrile 15 0:3040

General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.

label54

Extraction of LipidsFrom Chicken (Baked)From Chicken (Baked)

Food TestingEDGE

Note: Temperature and hold times may vary depending on the sample and analytes of interest.

Heating Program

Sample Weight9 g

Q-DiscC1

EquipmentEDGE40 mL collection vials - clear

NotesNone

Procedure1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc®.2. Weigh the sample into the Q-Cup.

Solvents(2:1) Chloroform/Methanol

SorbentsN/A

Wash Program

Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)

1 3030 4:00010

Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)

1 (2:1) Chloroform/Methanol 30 0:2030

2 (2:1) Chloroform/Methanol 30 0:0030

General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.

label55

Extraction of LipidsFrom Chicken (Fried)From Chicken (Fried)

Food TestingEDGE

Note: Temperature and hold times may vary depending on the sample and analytes of interest.

Heating Program

Sample Weight9 g

Q-DiscC1

EquipmentEDGE40 mL collection vials - clear

NotesNone

Procedure1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc®.2. Weigh the sample into the Q-Cup.

Solvents(2:1) Chloroform/Methanol

SorbentsN/A

Wash Program

Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)

1 3030 4:00010

Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)

1 (2:1) Chloroform/Methanol 15 0:2030

General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.

label56

Hydrolysis & Extraction of Total FatFrom ChocolateFrom Chocolate

Food TestingEDGE

Note: Temperature and hold times may vary depending on the sample and analytes of interest.

Heating Program

Sample Weight1 g

Q-DiscC9 + G1 + C9

EquipmentEDGE40 mL collection vials - clear

NotesStep 2- EDGE Method1. Assemble the Q-Cup with the Q-Disc®. Sandwich the G1 disc between the two C9 discs. 2. Place the Filter Paper into the Q-Cup.

ProcedureStep 1- Hydrolysis Method 1. Weigh out 1 g of milled or ground sample. 2. Add 1 mL Ethanol and 5 mL (25:11) Concentrated Hydrochloric Acid:Water. Heat for 1 hr at 100° C in a covered dish. 3. Add 5 mL of Ethanol. Filter the sample using Whatman 3 Filter Paper with a filter funnel, a vacuum pump, and filter flask. Wash any residual material from the beaker in the filter funnel using Ethanol. 4. Dry the filter paper in an oven at 100° C for 30 minutes. After the drying step, place Filter Paper into the Q-Cup® and extract.

SolventsExtraction:Petroleum Ether(25:11) Concentrated Hydrochloric Acid/WaterWash:Water

SorbentsN/A

Wash Program

Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)

1 14010 1:00010

2 14010 1:00010

Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)

1 Water 15 0:00100

2 Petroleum Ether 15 0:15100

General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.

label57

Hydrolysis & Extraction of Total FatFrom CookiesFrom Cookies

Food TestingEDGE

Note: Temperature and hold times may vary depending on the sample and analytes of interest.

Heating Program

Sample Weight1 g

Q-DiscC9 + G1 + C9

EquipmentEDGE40 mL collection vials - clear

NotesStep 2- EDGE Method1. Assemble the Q-Cup with the Q-Disc®. Sandwich the G1 disc between the two C9 discs. 2. Place the Filter Paper into the Q-Cup.

ProcedureStep 1- Hydrolysis Method 1. Weigh out 1 g of milled or ground sample. 2. Add 1 mL Ethanol and 5 mL (25:11) Concentrated Hydrochloric Acid:Water. Heat for 1 hr at 100° C in a covered dish. 3. Add 5 mL of Ethanol. Filter the sample using Whatman 3 Filter Paper with a filter funnel, a vacuum pump, and filter flask. Wash any residual material from the beaker in the filter funnel using Ethanol. 4. Dry the filter paper in an oven at 100° C for 30 minutes. After the drying step, place Filter Paper into the Q-Cup® and extract.

SolventsExtraction:Petroleum Ether(25:11) Concentrated Hyrdochloric Acid/WaterWash:Water

SorbentsN/A

Wash Program

Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)

1 14010 1:00010

2 14010 1:00010

Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)

1 Water 15 0:00150

2 Petroleum Ether 15 0:15150

General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.

label58

Extraction of FatFrom DATEM PowderFrom DATEM Powder

Food TestingEDGE

Note: Temperature and hold times may vary depending on the sample and analytes of interest.

Heating Program

Sample Weight1 g

Q-DiscC4

EquipmentEDGE40 mL collection vials - clear

NotesNone

Procedure1. Assemble the Q-Cup® with the Q-Disc®.2. Weigh the sample into the Q-Cup.3. Weigh the sand directly on top of the sample to compact down.

SolventsPetroleum Ether

SorbentsSand (1 g)

Wash Program

Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)

1 10010 1:0000

2 10010 1:0000

3 10010 1:0000

4 1005 1:0005

Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)

1 Petroluem Ether 15 0:15150

2 Petroluem Ether 15 0:00150

General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.

59

Extraction of FlavonoidsFrFromom Echinacea

Food TestingEDGE

label

Note: Temperature and hold times may vary depending on the sample and analytes of interest.

Heating Program

Sample Weight2 g

Q-DiscM2 + C9

EquipmentEDGE40 mL collection vials - clear

NotesNone

Procedure1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc®. Layer the M2 Q-Disc (textured side up) on top of theC9 Q-Disc.2. Weigh the sample into the Q-Cup.

Solvents(70:30) Methanol/Water

SorbentsN/A

Wash Program

Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)

1 6020 1:00020

Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)

1 (70:30) Methanol/Water 15 0:1060

2 (70:30) Methanol/Water 10 0:0060

General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtainrequired results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position andload method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.

label 60

Extraction of FatFrom Egg PowderFrom Egg Powder

Food TestingEDGE

Note: Temperature and hold times may vary depending on the sample and analytes of interest.

Heating Program

Sample Weight5 g

Q-DiscC4

EquipmentEDGE40 mL collection vials - clear

NotesNone

Procedure 1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc®. 2. Weigh the sample into the Q-Cup.

SolventsToluene

SorbentsN/A

Wash Program

Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)

1 14020 1:0000

2 14015 1:0005

Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)

1 Toluene 15 0:15150

2 Toluene 15 0:00150

General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.

label61

Extraction of Total FatFrom Feeds/FoodsFrom Feeds/Foods

Agriculture, Food TestingEDGE

Note: Temperature and hold times may vary depending on the sample and analytes of interest.

Heating Program

Sample Weight≤ 5 g

Q-DiscC1

EquipmentEDGE40 mL collection vials - clear

NotesNone

Procedure1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc®. 2. Weigh the sand into the Q-Cup. Spread evenly across the base of the Q-Cup by gentle tapping.3. Weigh the sample into the Q-Cup. Gently mix the sample and sand in the Q-Cup.4. Dry Q-Cups with sample and sand in the oven for 1 hour at 100° C while tilted at an angle in ~2" aluminum pans or equivalent. This provides stability and enables easier visual identification that no sample has been lost.

SolventsPetroleum Ether

SorbentsWet Samples Only: Sand (use equal amount to sample size)

Wash Program

Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)

1 14010 1:00010

2 14010 1:00010

Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)

1 Petroleum Ether 15 0:15150

2 Petroleum Ether 15 0:00150

General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.

label62

Extraction of PCBsFrom Feeds/Foods (Wet)From Feeds/Foods (Wet)

Agriculture, Food TestingEDGE

Note: Temperature and hold times may vary depending on the sample and analytes of interest.

Heating Program

Sample Weight5 g

Q-DiscC9 + G1 + C9

EquipmentEDGE40 mL collection vials - clear

NotesNone

Procedure1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc®. Sandwich the G1 disc between the two C9 discs.2. Weigh the sorbent into the Q-Cup. Spread evenly across the base of the Q-Cup by gentle tapping.3. Weigh the sample into the Q-Cup layering on top of the sorbent. No mixing is needed.

SolventsDichloromethane

SorbentsC18 (2.5 g)

Wash Program

Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)

1 13520 2:00510

Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)

1 Dichloromethane 15 0:15150

2 Dichloromethane 15 0:00150

General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.

label63

Extraction of DioxinsFrom Feeds/Foods (Wet)From Feeds/Foods (Wet)

Agriculture, Food TestingEDGE

Note: Temperature and hold times may vary depending on the sample and analytes of interest.

Heating Program

Sample Weight5 g

Q-DiscC4

EquipmentEDGE60 mL collection vials - clear

NotesNone

Procedure1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc®. 2. Weigh the sorbent into the Q-Cup. Spread evenly across the base of the Q-Cup by gentle tapping.3. Weigh the sample into the Q-Cup layering on top of the sorbent. No mixing is needed.

SolventsOptions:DichloromethaneMethyl tert-butyl etherToluene

SorbentsC18 (2 g)

Wash Program

Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)

1 15020 1:0000

2 15020 1:0000

3 15020 1:0000

Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)

1 Extraction Solvent 30 0:30150

2 Extraction Solvent 30 0:30150

General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.

label64

Extraction of PCBsFrom Fish Meal (Dry)From Fish Meal (Dry)

Agriculture, Food TestingEDGE

Note: Temperature and hold times may vary depending on the sample and analytes of interest.

Heating Program

Sample Weight5 g

Q-DiscC1M1 + C9

EquipmentEDGE40 mL collection vials - clear

NotesLess than 5 grams of sample can be used with a ratio of 1:2 C18: Sample.Either C1 or M1/C9 Q-Disc can be used.

Procedure1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc®. If using the M1 disc, layer a C9 disc on top of M1.2. Weigh the sorbent into the Q-Cup. Spread evenly across the base of the Q-Cup by gentle tapping.3. Weigh the sample into the Q-Cup layering the sample on top of the sorbent. No mixing is required.

SolventsOptions:Dichloromethane(1:1) Hexane/Acetone

SorbentsC18 (2.5 g)

Wash Program

Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)

1 12020 3:001010

Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)

1 Dichloromethane 15 0:15150

2 Extraction Solvent 10 0:00150

General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.

label65

Extraction of PAHsFrom Fish Tissue (Dry)From Fish Tissue (Dry)

Agriculture, Food Testing, NutraceuticalEDGE

Note: Temperature and hold times may vary depending on the sample and analytes of interest.

Heating Program

Sample Weight5 g

Q-DiscC1

EquipmentEDGE40 mL collection vials - clear

NotesNone

Procedure1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc®.2. Weigh the sorbent in the Q-Cup. Tap lightly to spread evenly.3. Weigh the sample into the Q-Cup layering the sample on top of the sorbent. No mixing is required.

SolventsDichloromethane

SorbentsC18 (1.7 g)

Wash Program

Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)

1 12020 3:001010

Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)

1 Dichloromethane 15 0:30150

2 Dichloromethane 15 0:00150

General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.

label66

Extraction of PAHsFrom Fish Tissue (Wet)From Fish Tissue (Wet)

Agriculture, Environmental and RegulatEDGE

Note: Temperature and hold times may vary depending on the sample and analytes of interest.

Heating Program

Sample Weight2 g

Q-DiscC9 + G1 + C9

EquipmentEDGE40 mL collection vials - clear

NotesIf there is moisture in collection vial after the extraction:Using a glass funnel with glass wool and about 20 g of Sodium Sulfate. Pour over into new collection vial and rinse thoroughly with fresh solvent.

Procedure1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc®. Sandwich the G1 disc between the two C9 discs.2. Weigh the sorbent into the Q-Cup. Spread evenly across the base of the Q-Cup by gentle tapping.3. Weigh the sample into the Q-Cup layering on top of the sorbent. No mixing is needed.

SolventsOptions:Dichloromethane(1:1) Hexane/Acetone

SorbentsC18 (0.8 g)

Wash Program

Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)

1 12020 3:001010

Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)

1 Extraction Solvent 15 0:15150

2 Extraction Solvent 15 0:00150

General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.

label67

Extraction of PesticidesFrom Foods (Wet)From Foods (Wet)

Agriculture, Food TestingEDGE

Note: Temperature and hold times may vary depending on the sample and analytes of interest.

Heating Program

Sample Weight5 g

Q-DiscC9 + G1 + C9

EquipmentEDGE40 mL collection vials - clear

NotesNone

Procedure1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc®. Sandwich the G1 disc between two C9 discs.2. Weigh the sorbents into the Q-Cup. Spread evenly across the sample by gentle tapping.3. Weigh the sample into the Q-Cup layering the sample on top of the sorbents. No mixing is required.

Solvents1% Acetic Acid in Acetonitrile

SorbentsQ-Matrix Hydraᵀᴹ (2.5 g)

Wash Program

Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)

1 4020 3:00010

Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)

1 1% Acetic Acid in Acetonitrile 15 0:1540

2 1% Acetic Acid in Acetonitrile 15 0:0040

General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.

label68

ExtractionFrom Goldenseal RootFrom Goldenseal Root

Food Testing, NutraceuticalsEDGE

Note: Temperature and hold times may vary depending on the sample and analytes of interest.

Heating Program

Sample Weight5 g

Q-DiscC1

EquipmentEDGE40 mL collection vials - clear

NotesNone

Procedure1. Assemble the Q-Cup® with the Q-Disc®.2. Weigh the sample into the Q-Cup.

SolventsAcetonitrile

SorbentsN/A

Wash Program

Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)

1 10015 1:00105

Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)

1 Acetonitrile 15 0:15150

2 Acetonitrile 15 0:00150

General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.

label69

Extraction of PesticidesFrom HopsFrom Hops

Agriculture, Food TestingEDGE

Note: Temperature and hold times may vary depending on the sample and analytes of interest.

Heating Program

Sample Weight0.5 g

Q-DiscM2 + C9

EquipmentEDGE40 mL collection vials - clear

NotesCanadian Pesticide Mix 4 in LCMS grade obtained from SPEX CertiPrep.

Procedure1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc®. Layer the M2 Q-Disc (textured side up) on top of the C9 Q-Disc.2. Add sorbents to bottom of Q-Cup. Spread evenly across the base of the Q-Cup by gentle tapping. No mixing is required.3. Weigh the sample into the Q-Cup layering on top of the sorbent. No mixing is required.4. Spike with 100 ug/mL Canadian Pesticide Mix 4 and leave in hood for approximately 30 minutes to evaporate solvent.

Solvents1% Acetic Acid in Acetonitrile

SorbentsQ-Matrix Hydraᵀᴹ (1.25 g)Supelᵀᴹ QuE Citrate (1.25 g)Primary secondary amine (1.25 g)Siliabond C18 (1.25 g)*Different sorbents can be used

Wash Program

Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)

1 4015 3:00510

Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)

1 1% Acetic Acid in Acetonitrile 15 0:0040

2 1% Acetic Acid in Acetonitrile 15 0:0040

General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.

label70

Extraction of Total FatFrom Hot DogsFrom Hot Dogs

Food TestingEDGE

Note: Temperature and hold times may vary depending on the sample and analytes of interest.

Heating Program

Sample Weight2 g

Q-DiscC1

EquipmentEDGE40 mL collection vials - clear

NotesNone

Procedure1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc®. 2. Weigh the sand into the Q-Cup. Spread evenly across the base of the Q-Cup by gentle tapping.3. Weigh the sample into the Q-Cup. Gently mix the sample and the sand in the Q-Cup.4. Dry Q-Cups with the sample and the sand in the oven for 1 hour at 100° C while tilted at an angle in ~2" aluminum pans or equivalent. This provides stability and enables easier visual identification that no sample has been lost.

SolventsExtraction:Petroleum EtherWash:Water

SorbentsSand (2 g)

Wash Program

Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)

1 14010 1:00010

2 14010 1:00010

Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)

1 Water 10 0:00150

2 Petroleum Ether 15 0:00150

General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.

label71

Extraction of PAHsFrom Infant FormulaFrom Infant Formula

Food Testing, Nutraceuticals, Personal EDGE

Note: Temperature and hold times may vary depending on the sample and analytes of interest.

Heating Program

Sample Weight4 g

Q-DiscC9 + G1 + C9

EquipmentEDGE40 mL collection vials - clear

NotesNone

Procedure1. Assemble the Q-Cup® with the Q-Disc®. Sandwich the G1 disc between two C9 discs.2. Weigh the sample into the Q-Cup.

SolventsCyclohexane

SorbentsN/A

Wash Program

Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)

1 12020 1:00010

Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)

1 Cyclohexane 15 0:30150

2 Cyclohexane 15 0:00150

General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.

label72

Extraction of Total FatFrom Meats (High-Fat)From Meats (High-Fat)

Food TestingEDGE

Note: Temperature and hold times may vary depending on the sample and analytes of interest.

Heating Program

Sample Weight2 g

Q-DiscC1

EquipmentEDGE40 mL collection vials - clear

NotesNone

Procedure1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc®. 2. Weigh the sand into the Q-Cup. Spread evenly across the base of the Q-Cup by gentle tapping.3. Weigh the sample into the Q-Cup. Gently mix the sample and sand in the Q-Cup.4. Dry Q-Cups with the sample and the sand in the oven for 1 hour at 100° C while tilted at an angle in ~2" aluminum pans or equivalent. This provides stability and enables easier visual identification that no sample has been lost.

SolventsPetroleum Ether

SorbentsSand (2 g)

Wash Program

Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)

1 14010 1:00010

2 14010 1:00010

Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)

1 Petroleum Ether 15 0:15150

2 Petroleum Ether 15 0:00150

General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.

label73

Extraction of Total FatFrom Meats (Potted)From Meats (Potted)

Food TestingEDGE

Note: Temperature and hold times may vary depending on the sample and analytes of interest.

Heating Program

Sample Weight2 g

Q-DiscC1

EquipmentEDGE40 mL collection vials - clear

NotesNone

Procedure1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc®. 2. Weigh the sand into the Q-Cup. Spread evenly across the base of the Q-Cup by gentle tapping.3. Weigh the sample into the Q-Cup. Gently mix the sample and the sand in the Q-Cup.4. Dry Q-Cups with the sample and the sand in the oven for 1 hour at 100° C while tilted at an angle in ~2" aluminum pans or equivalent. This provides stability and enables easier visual identification that no sample has been lost.

SolventsExtraction:Petroleum EtherWash:Water

SorbentsSand (2 g)

Wash Program

Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)

1 14010 1:00010

2 14010 1:00010

Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)

1 Water 10 0:00150

2 Petroleum Ether 15 0:15150

General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.

label74

Extraction of FatFrom Nuts (Brazil)From Nuts (Brazil)

Agriculture, Food TestingEDGE

Note: Temperature and hold times may vary depending on the sample and analytes of interest.

Heating Program

Sample Weight12 g

Q-DiscC1

EquipmentEDGE40 mL collection vials - clear

NotesNone

Procedure1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc®. 2. Weigh the sand into the Q-Cup. Spread evenly across the base of the Q-Cup by gentle tapping.3. Weigh the sample into the Q-Cup. Gently mix the sample and the sand in the Q-Cup.

SolventsPetroleum Ether

SorbentsSand (12 g)

Wash Program

Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)

1 14020 1:0000

2 14015 1:0005

Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)

1 Petroleum Ether 15 0:15150

2 Petroleum Ether 15 0:00150

General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.

label75

Extraction of FatFrom Nuts (Cashews)From Nuts (Cashews)

Food TestingEDGE

Note: Temperature and hold times may vary depending on the sample and analytes of interest.

Heating Program

Sample Weight12 g

Q-DiscC1

EquipmentEDGE40 mL collection vials - clear

NotesNone

Procedure1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc®.2. Weigh the sand into the Q-Cup. Spread evenly across the base of the Q-Cup by gentle tapping.3. Weigh the sample into the Q-Cup. Gently mix the sample and sand in the Q-Cup.

SolventsPetroleum Ether

SorbentsSand (12 g)

Wash Program

Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)

1 14020 1:0000

2 14015 1:0005

Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)

1 Petroleum Ether 15 0:15150

2 Petroleum Ether 15 0:00150

General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.

label76

Extraction of FatFrom Nuts (Hazelnuts)From Nuts (Hazelnuts)

Agriculture, Food TestingEDGE

Note: Temperature and hold times may vary depending on the sample and analytes of interest.

Heating Program

Sample Weight12 g

Q-DiscC1

EquipmentEDGE40 mL collection vials - clear

NotesNone

Procedure1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc®. 2. Weigh the sand into the Q-Cup. Spread evenly across the base of the Q-Cup by gentle tapping.3. Weigh the sample into the Q-Cup. Gently mix the sample and sand in the Q-Cup.

SolventsPetroleum Ether

SorbentsSand (12 g)

Wash Program

Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)

1 14020 1:0000

2 14015 1:0005

Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)

1 Petroleum Ether 15 0:15150

2 Petroleum Ether 0 0:00150

General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.

label77

Extraction of Total FatFrom Nuts (Peanuts)From Nuts (Peanuts)

Food TestingEDGE

Note: Temperature and hold times may vary depending on the sample and analytes of interest.

Heating Program

Sample Weight12 g

Q-DiscC1

EquipmentEDGE40 mL collection vials - clear

NotesNone

Procedure1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc®. 2. Weigh the sand into the Q-Cup. Spread evenly across the base of the Q-Cup by gentle tapping.3. Weigh the sample into the Q-Cup. Gently mix the sample and the sand in the Q-Cup.4. Dry Q-Cups with the sample and the sand in the oven for 1 hour at 100° C while tilted at an angle in ~2" aluminum pans or equivalent. This provides stability and enables easier visual identification that no sample has been lost.

SolventsPetroleum Ether

SorbentsSand (12 g)

Wash Program

Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)

1 14020 1:0000

2 14015 1:0005

Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)

1 Petroleum Ether 15 0:15150

2 Petroleum Ether 15 0:00150

General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.

label78

Extraction of Total FatFrom PepperoniFrom Pepperoni

Food TestingEDGE

Note: Temperature and hold times may vary depending on the sample and analytes of interest.

Heating Program

Sample Weight2 g

Q-DiscC1

EquipmentEDGE40 mL collection vials - clear

NotesNone

Procedure1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc®. 2. Weigh the sand into the Q-Cup. Spread evenly across the base of the Q-Cup by gentle tapping.3. Weigh the sample into the Q-Cup. Gently mix the sample and the sand in the Q-Cup.4. Dry Q-Cups with the sample and the sand in the oven for 1 hour at 100° C while tilted at an angle in ~2" aluminum pans or equivalent. This provides stability and enables easier visual identification that no sample has been lost.

SolventsPetroleum Ether

SorbentsSand (2 g)

Wash Program

Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)

1 14010 1:00010

2 14010 1:00010

Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)

1 Water 10 0:00150

2 Petroleum Ether 15 0:15150

General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.

label79

Extraction of LipidsFrom Potatoes (Baked)From Potatoes (Baked)

Food TestingEDGE

Note: Temperature and hold times may vary depending on the sample and analytes of interest.

Heating Program

Sample Weight9 g

Q-DiscC1

EquipmentEDGE40 mL collection vials - clear

NotesNone

Procedure1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc®.2. Weigh the sample into the Q-Cup.

Solvents(3:2) Hexane/Isopropanol

SorbentsN/A

Wash Program

Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)

1 3030 4:00010

Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)

1 (3:2) Hexane/Isopropanol 30 0:2030

2 (3:2) Hexane/Isopropanol 30 0:0030

General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.

label80

Extraction of LipidsFrom Potatoes (Fried)From Potatoes (Fried)

Food TestingEDGE

Note: Temperature and hold times may vary depending on the sample and analytes of interest.

Heating Program

Sample Weight9 g

Q-DiscC1

EquipmentEDGE40 mL collection vials - clear

NotesNone

Procedure1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc®.2. Weigh the sample into the Q-Cup.

Solvents(3:2) Hexane/Isopropanol

SorbentsN/A

Wash Program

Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)

1 3030 4:00010

Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)

1 (3:2) Hexane/Isopropanol 15 0:2030

General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.

label81

Extraction of NutrientsFrom Protein PowderFrom Protein Powder

Food Testing, NutraceuticalsEDGE

Note: Temperature and hold times may vary depending on the sample and analytes of interest.

Heating Program

Sample Weight1 g

Q-DiscC4

EquipmentEDGE40 mL collection vials - clear

NotesNone

Procedure1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc®. 2. Weigh the sand into the Q-Cup. Spread evenly across the base of the Q-Cup by gentle tapping.3. Weigh the sample into the Q-Cup. Gently mix the sample and the sand in the Q-Cup.

Solvents(80:20) Methanol/Water

SorbentsSand (3 g)

Wash Program

Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)

1 15015 0:101010

Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)

1 (80:20) Methanol/Water 15 0:15150

2 (80:20) Methanol/Water 15 0:00150

General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.

label82

Extraction of Total FatFrom Protein PowderFrom Protein Powder

Food Testing, NutraceuticalsEDGE

Note: Temperature and hold times may vary depending on the sample and analytes of interest.

Heating Program

Sample Weight2 g

Q-DiscC1

EquipmentEDGE40 mL collection vials - clear

NotesNone

Procedure1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc®. 2. Weigh the sand into the Q-Cup. Spread evenly across the base of the Q-Cup by gentle tapping.3. Weigh the sample into the Q-Cup. Gently mix the sample and the sand in the Q-Cup.

SolventsPetroleum Ether

SorbentsSand (6 g)

Wash Program

Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)

1 12015 2:00105

Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)

1 Petroleum Ether 15 0:15150

2 Petroleum Ether 15 0:00150

General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.

label83

Extraction of FatFrom Pumpkin SeedsFrom Pumpkin Seeds

Agriculture, Food TestingEDGE

Note: Temperature and hold times may vary depending on the sample and analytes of interest.

Heating Program

Sample Weight12 g

Q-DiscC1

EquipmentEDGE40 mL collection vials - clear

NotesNone

Procedure1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc®. 2. Weigh the sand into the Q-Cup. Spread evenly across the base of the Q-Cup by gentle tapping.3. Weigh the sample into the Q-Cup. Gently mix the sample and sand in the Q-Cup.

SolventsPetroleum Ether

SorbentsSand (12 g)

Wash Program

Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)

1 14020 1:0000

2 14015 1:0005

Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)

1 Petroleum Ether 15 0:15150

2 Petroleum Ether 15 0:00150

General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.

label84

Extraction of PesticidesFrom RiceFrom Rice

Agriculture, Food TestingEDGE

Note: Temperature and hold times may vary depending on the sample and analytes of interest.

Heating Program

Sample Weight5 g

Q-DiscM2 + C9

EquipmentEDGE40 mL collection vials - clear

NotesNone

Procedure1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc®. Layer the M2 Q-Disc (textured side up) on top of the C9 Q-Disc. 2. Weigh the sorbents into the Q-Cup. Spread evenly across the sample by gentle tapping.3. Weigh the sample into the Q-Cup layering the sample on top of the sorbent. No mixing is required.

Solvents1% Acetic Acid in Acetonitile

SorbentsQ-Matrix Hydraᵀᴹ (2.5 g)

Wash Program

Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)

1 4020 3:00010

Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)

1 1% Acetic Acid in Acetonitrile 15 0:1540

2 1% Acetic Acid in Acetonitrile 15 0:0040

General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.

label85

Extraction of PCBsFrom Soy Meal (Dry)From Soy Meal (Dry)

Agriculture, Food TestingEDGE

Note: Temperature and hold times may vary depending on the sample and analytes of interest.

Heating Program

Sample Weight5 g

Q-DiscC1M1 + C9

EquipmentEDGE40 mL collection vials - clear

NotesLess than 5 grams of sample can be used with a ratio of 1:2 C18/Sample.Either C1 or M1/C9 Q-Disc can be used.

Procedure1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc®. If using M1 disc, layer on top of C9 disc.2. Weigh the sorbent into the Q-Cup. Spread evenly across the base of the Q-Cup by gentle tapping.3. Weigh the sample into the Q-Cup layering the sample on top of the sorbent. No mixing is required.

SolventsOptions:Dichloromethane(1:1) Hexane/Acetone

SorbentsC18 (2.5 g)

Wash Program

Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)

1 12020 3:001010

Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)

1 Dichloromethane 15 0:15150

2 Extraction Solvent 10 0:00150

General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.

label86

Extraction of PesticidesFrom Spices (Dry)From Spices (Dry)

Agriculture, Food TestingEDGE

Note: Temperature and hold times may vary depending on the sample and analytes of interest.

Heating Program

Sample Weight1 g

Q-DiscC9 + G1 + C9

EquipmentEDGE40 mL collection vials - clear

NotesMatrix matched calibration curve required without the use of sorbents.

Procedure1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc®. Sandwich the G1 disc between the two C9 discs.2. Weigh the sample into the Q-Cup.

Solvents1% Acetic Acid in Acetonitrile

SorbentsN/A

Wash Program

Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)

1 4015 3:00510

Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)

1 1% Acetic Acid in Acetonitrile 15 0:1040

2 1% Acetic Acid in Acetonitrile 15 0:0040

General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.

label87

ExtractionFrom Spirulina AlgaeFrom Spirulina Algae

Food Testing, NutraceuticalsEDGE

Note: Temperature and hold times may vary depending on the sample and analytes of interest.

Heating Program

Sample Weight2 g

Q-DiscC1

EquipmentEDGE40 mL collection vials - clear

NotesNone

Procedure1. Assemble the Q-Cup® with the Q-Disc®.

2. Weigh the sample into the Q-Cup.

SolventsAcetonitrile

SorbentsN/A

Wash Program

Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)

1 10015 1:00105

Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)

1 Acetonitrile 15 0:00150

2 Acetonitrile 15 0:00150

General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.

label88

Extraction of PesticidesFrom StrawberriesFrom Strawberries

Agriculture, Food TestingEDGE

Note: Temperature and hold times may vary depending on the sample and analytes of interest.

Heating Program

Sample Weight5 g

Q-DiscC9 + G1 + C9

EquipmentEDGE40 mL collection vials - clear

NotesNone

Procedure1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc®. Sandwich the G1 disc between 2 C9 Q-Disc. 2. Weigh the sorbents into the Q-Cup. Spread evenly across the sample by gentle tapping.3. Weigh the sample into the Q-Cup layering the sample on top of the sorbent. No mixing is required.

Solvents1% Acetic Acid in Acetonitrile

SorbentsQ-Matrix Hydraᵀᴹ (2.5 g)Primary Second Amine (2 g)

Wash Program

Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)

1 4020 3:00010

Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)

1 1% Acetic Acid in Acetonitrile 15 0:1040

2 1% Acetic Acid in Acetonitrile 15 0:0040

General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.

label89

Extraction of PAHsFrom Yerba MateFrom Yerba Mate

Food Testing, NutraceuticalsEDGE

Note: Temperature and hold times may vary depending on the sample and analytes of interest.

Heating Program

Sample Weight0.5 g

Q-DiscC1

EquipmentEDGE40 mL collection vials - clear

NotesNone

Procedure 1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc®. 2. Weigh the sample into the Q-Cup.

SolventsOptions:(1:1) Hexane/AcetoneDichloromethaneAcetone

SorbentsN/A

Wash Program

Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)

1 120-15015 1:00105

Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)

1 Extraction Solvent 15 0:30150

2 Extraction Solvent 15 0:00150

General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.

label90

Geoscience & Mining

91

Extraction of DioxinsFrom Calcined Kiln DustFrom Calcined Kiln Dust

Environmental and Regulatory, GeoscieEDGE

Note: Temperature and hold times may vary depending on the sample and analytes of interest.

Heating Program

Sample Weight10 g

Q-DiscC9 + G1 + C9

EquipmentEDGE60 mL collection vials - amber

NotesNone

Procedure1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup®. Sandwich the G1 Q-Disc® between the two C9 Q-Discs. 2. Weigh the sample into the Q-Cup.

SolventsOptions:DichloromethaneMethyl tert-butyl ether

SorbentsN/A

Wash Program

Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)

1 15020 2:0000

2 15020 2:0000

3 15020 2:0000

Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)

1 Dichloromethane 20 0:30150

2 Methyl tert-butyl ether 20 0:30150

General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.

label92

Extraction of SVOCs, Pesticides, TPH, PCBFrom Cement MixFrom Cement Mix

Environmental and Regulatory, GeoscieEDGE

Note: Temperature and hold times may vary depending on the sample and analytes of interest.

Heating Program

Sample Weight15 g

Q-DiscC9 + G1 + C9

EquipmentEDGE40 mL collection vials - clear

NotesNone

Procedure1. Assemble the Q-Cup® with the Q-Disc®. Sandwich the G1 disc between two C9 discs. 2. Weigh the sample into the Q-Cup.

Solvents(1:1) Acetone/Dichloromethane

SorbentsN/A

Wash Program

Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)

1 10020 3:001010

Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)

1 (1:1) Acetone/Dichloromethane 15 0:30150

2 (1:1) Acetone/Dichloromethane 15 0:00150

General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.

label93

Extraction ofMolybdenumMolybdenum

Geoscience and Mining, Metals and AllEDGE

Note: Temperature and hold times may vary depending on the sample and analytes of interest.

Heating Program

Sample Weight2 g

Q-DiscC1

EquipmentEDGE40 mL collection vials - clear

NotesNone

Procedure 1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc®. 2. Weigh the sample into the Q-Cup.

SolventsAcetone

SorbentsN/A

Wash Program

Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)

1 14015 1:00105

Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)

1 Acetone 15 0:15140

2 Acetone 15 0:00140

General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.

label94

Industrial Hygiene

95

Extraction of PCBsFrom PUF Filters (Large)From PUF Filters (Large)

Environmental and Regulatory, IndustriaEDGE

Note: Temperature and hold times may vary depending on the sample and analytes of interest.

Heating Program

Sample WeightEntire Filter

Q-DiscC1

EquipmentEDGE40 mL collection vials - clear

NotesNone

Procedure 1. Assemble the Q-Cup® with the Q-Disc®. 2. Compress and push the filter into the base of the Q-Cup.

SolventsHexane

SorbentsN/A

Wash Program

Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)

1 11520 3:00155

Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)

1 Hexane 10 0:15150

2 Hexane 10 0:00150

General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.

label96

Extraction of PCBsFrom PUF Filters (Small)From PUF Filters (Small)

Environmental and Regulatory, IndustriaEDGE

Note: Temperature and hold times may vary depending on the sample and analytes of interest.

Heating Program

Sample WeightEntire Filter

Q-DiscC1

EquipmentEDGE40 mL collection vials - clear

NotesNone

Procedure1. Assemble the Q-Cup® with the Q-Disc®. 2. Compress and push the filter into the base of the Q-Cup.

SolventsHexane

SorbentsN/A

Wash Program

Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)

1 10015 0:10100

Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)

1 Hexane 10 0:15150

2 Hexane 10 0:00150

General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.

label97

Extraction of BrodifacoumFrom Rat Poison PowderFrom Rat Poison Powder

Agriculture, Industrial Hygiene, MaterialEDGE

Note: Temperature and hold times may vary depending on the sample and analytes of interest.

Heating Program

Sample Weight2 g

Q-DiscC4

EquipmentEDGE40 mL collection vials - clear

NotesNone

Procedure 1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc®. 2. Weigh the sample into the Q-Cup.

SolventsMethanol

SorbentsN/A

Wash Program

Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)

1 13015 2:001010

2 13015 2:001010

Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)

1 Methanol 15 0:15150

2 Methanol 15 0:00150

General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.

label98

Cleaning ofPUF FiltersPUF Filters

Environmental and Regulatory, IndustriaEDGE

Note: Temperature and hold times may vary depending on the sample and analytes of interest.

Heating Program

Sample WeightEntire Filter

Q-DiscC1

EquipmentEDGE40 mL collection vials - clear

NotesNone

Procedure 1. Assemble the Q-Cup® with the Q-Disc®. 2. Compress and push the filter into the base of the Q-Cup.

SolventsOptions:HexaneAcetone

SorbentsN/A

Wash Program

Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)

1 11510 3:00100

2 11510 3:00100

3 11530 3:00100

Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)

1 Hexane 15 0:15150

2 Acetone 15 0:00150

General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.

label99

Materials Science

100

Extraction ofFilm PowderFilm Powder

Materials Science, Plastics, Polymers, aEDGE

Note: Temperature and hold times may vary depending on the sample and analytes of interest.

Heating Program

Sample Weight1 g

Q-DiscM1 + C9

EquipmentEDGE40 mL collection vials - clear

Notes*Plastics and polymers should only be extracted at temperatures below melting point range.Expansion of the sample during extraction is common during polymer based extraction.

Procedure 1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc. Layer the M1 disc on top of the C9 disc. 2. Weigh the sample into the Q-Cup.

SolventsOptions:CyclohexaneDichloromethane

SorbentsN/A

Wash Program

Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)

1 *40-7015 10:0005

Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)

1 Cyclohexane 15 0:0070

2 Dichloromethane 15 0:1570

General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.

label101

Extraction of PigmentsFrom PlasticsFrom Plastics

Materials Science, Plastics, Polymers, aEDGE

Note: Temperature and hold times may vary depending on the sample and analytes of interest.

Heating Program

Sample Weight1 g

Q-DiscM1 + C9

EquipmentEDGE40 mL collection vials - clear

Notes*Plastics and polymers should only be extracted at temperatures below melting point range.Expansion of the sample during extraction is common during polymer based extraction.

Procedure 1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc. Layer the M1 disc on top of the C9 disc. 2. Weigh the sample into the Q-Cup.

SolventsOptions:CyclohexaneDichloromethane

SorbentsN/A

Wash Program

Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)

1 *40-8015 10:0005

Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)

1 Cyclohexane 10 0:1580

2 Dichloromethane 10 0:0080

General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.

label102

Extraction of AdditivesFrom Polypropylene PowderFrom Polypropylene Powder

Materials Science, Plastics, Polymers, aEDGE

Note: Temperature and hold times may vary depending on the sample and analytes of interest.

Heating Program

Sample Weight1 g

Q-DiscC9 + G1 + C9

EquipmentEDGE40 mL collection vials - clear

Notes*Plastics and polymers should only be extracted at temperatures below melting point range.Expansion of the sample during extraction is common during polymer based extraction.

Procedure1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc®. Sandwich the G1 disc between the two C9 discs. 2. Weigh the sample into the Q-Cup.

SolventsIsopropanol

SorbentsN/A

Wash Program

Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)

1 12030 15:00010

Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)

1 Isopropanol 15 0:15150

2 Isopropanol 15 0:00150

General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.

label103

Extraction of PhthalatesFrom Polyvinyl ChlorideFrom Polyvinyl Chloride

Materials Science, Plastics, Polymers, aEDGE

Note: Temperature and hold times may vary depending on the sample and analytes of interest.

Heating Program

Sample Weight1 g

Q-DiscC9 + G1 + C9

EquipmentEDGE40 mL collection vials - clear

Notes*Plastics and polymers should only be extracted at temperatures below melting point range.Expansion of the sample during extraction is common during polymer based extraction.

Procedure 1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc. Sandwich the G disc between 2 C9 discs. 2. Weigh the sample into the Q-Cup

Solvents(1:1) Isopropanol/Cyclohexane

SorbentsN/A

Wash Program

Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)

1 *8020 10:00010

Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)

1 (1:1) Isopropanol/Cyclohexane 10 0:1580

2 (1:1) Isopropanol/Cyclohexane 10 0:0080

General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.

label104

Extraction of PCBsFrom PUF Filters (Large)From PUF Filters (Large)

Environmental and Regulatory, IndustriaEDGE

Note: Temperature and hold times may vary depending on the sample and analytes of interest.

Heating Program

Sample WeightEntire Filter

Q-DiscC1

EquipmentEDGE40 mL collection vials - clear

NotesNone

Procedure 1. Assemble the Q-Cup® with the Q-Disc®. 2. Compress and push the filter into the base of the Q-Cup.

SolventsHexane

SorbentsN/A

Wash Program

Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)

1 11520 3:00155

Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)

1 Hexane 10 0:15150

2 Hexane 10 0:00150

General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.

label105

Extraction of PCBsFrom PUF Filters (Small)From PUF Filters (Small)

Environmental and Regulatory, IndustriaEDGE

Note: Temperature and hold times may vary depending on the sample and analytes of interest.

Heating Program

Sample WeightEntire Filter

Q-DiscC1

EquipmentEDGE40 mL collection vials - clear

NotesNone

Procedure1. Assemble the Q-Cup® with the Q-Disc®. 2. Compress and push the filter into the base of the Q-Cup.

SolventsHexane

SorbentsN/A

Wash Program

Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)

1 10015 0:10100

Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)

1 Hexane 10 0:15150

2 Hexane 10 0:00150

General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.

label106

Extraction of BrodifacoumFrom Rat Poison PowderFrom Rat Poison Powder

Agriculture, Industrial Hygiene, MaterialEDGE

Note: Temperature and hold times may vary depending on the sample and analytes of interest.

Heating Program

Sample Weight2 g

Q-DiscC4

EquipmentEDGE40 mL collection vials - clear

NotesNone

Procedure 1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc®. 2. Weigh the sample into the Q-Cup.

SolventsMethanol

SorbentsN/A

Wash Program

Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)

1 13015 2:001010

2 13015 2:001010

Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)

1 Methanol 15 0:15150

2 Methanol 15 0:00150

General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.

label107

Extraction of AntioxidantsFrom ResinFrom Resin

Materials ScienceEDGE

Note: Temperature and hold times may vary depending on the sample and analytes of interest.

Heating Program

Sample Weight1 g

Q-DiscC1

EquipmentEDGE40 mL collection vials - clear

NotesNone

Procedure 1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc®. 2. Weigh the sample into the Q-Cup.

SolventsMethanol

SorbentsN/A

Wash Program

Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)

1 10015 2:00510

Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)

1 Methanol 15 0:20150

General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.

label108

Extraction of PAHsFrom XAD ResinFrom XAD Resin

Materials ScienceEDGE

Note: Temperature and hold times may vary depending on the sample and analytes of interest.

Heating Program

Sample Weight15 g

Q-DiscC1

EquipmentEDGE40 mL collection vials - clear

NotesBe sure that sample or sand is present in the Q-Cup when running this method. Sample size does matter, as you decrease the sample size you will build up more pressure within the cell.

Procedure 1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc®. 2. Weigh the sample into the Q-Cup.

SolventsDichloromethane

SorbentsN/A

Wash Program

Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)

1 15020 2:001010

Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)

1 Dichloromethane 15 0:30150

2 Dichloromethane 15 0:00150

General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.

label109

Cleaning ofPUF FiltersPUF Filters

Environmental and Regulatory, IndustriaEDGE

Note: Temperature and hold times may vary depending on the sample and analytes of interest.

Heating Program

Sample WeightEntire Filter

Q-DiscC1

EquipmentEDGE40 mL collection vials - clear

NotesNone

Procedure 1. Assemble the Q-Cup® with the Q-Disc®. 2. Compress and push the filter into the base of the Q-Cup.

SolventsOptions:HexaneAcetone

SorbentsN/A

Wash Program

Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)

1 11510 3:00100

2 11510 3:00100

3 11530 3:00100

Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)

1 Hexane 15 0:15150

2 Acetone 15 0:00150

General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.

label110

Metals & Alloys

111

Extraction ofMolybdenumMolybdenum

Geoscience and Mining, Metals and AllEDGE

Note: Temperature and hold times may vary depending on the sample and analytes of interest.

Heating Program

Sample Weight2 g

Q-DiscC1

EquipmentEDGE40 mL collection vials - clear

NotesNone

Procedure 1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc®. 2. Weigh the sample into the Q-Cup.

SolventsAcetone

SorbentsN/A

Wash Program

Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)

1 14015 1:00105

Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)

1 Acetone 15 0:15140

2 Acetone 15 0:00140

General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.

label112

Nutraceuticals

113

Extraction of FlavonoidsFrom EchinaceaFrom Echinacea

Food Testing, NutraceuticalsEDGE

Note: Temperature and hold times may vary depending on the sample and analytes of interest.

Heating Program

Sample Weight2 g

Q-DiscM2 + C9

EquipmentEDGE40 mL collection vials - clear

NotesNone

Procedure1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc®. Layer the M2 Q-Disc (textured side up) on top of the C9 Q-Disc.2. Weigh the sample into the Q-Cup.

Solvents(70:30) Methanol/Water

SorbentsN/A

Wash Program

Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)

1 6020 1:00020

Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)

1 (70:30) Methanol/Water 15 0:1060

2 (70:30) Methanol/Water 10 0:0060

General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.

label114

Extraction of PAHsFrom Fish Tissue (Dry)From Fish Tissue (Dry)

Agriculture, Food Testing, NutraceuticalEDGE

Note: Temperature and hold times may vary depending on the sample and analytes of interest.

Heating Program

Sample Weight5 g

Q-DiscC1

EquipmentEDGE40 mL collection vials - clear

NotesNone

Procedure1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc®.2. Weigh the sorbent in the Q-Cup. Tap lightly to spread evenly.3. Weigh the sample into the Q-Cup layering the sample on top of the sorbent. No mixing is required.

SolventsDichloromethane

SorbentsC18 (1.7 g)

Wash Program

Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)

1 12020 3:001010

Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)

1 Dichloromethane 15 0:30150

2 Dichloromethane 15 0:00150

General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.

label115

Extraction of PAHsFrom Fish Tissue (Wet)From Fish Tissue (Wet)

Agriculture, Environmental and RegulatEDGE

Note: Temperature and hold times may vary depending on the sample and analytes of interest.

Heating Program

Sample Weight2 g

Q-DiscC9 + G1 + C9

EquipmentEDGE40 mL collection vials - clear

NotesIf there is moisture in collection vial after the extraction:Using a glass funnel with glass wool and about 20 g of Sodium Sulfate. Pour over into new collection vial and rinse thoroughly with fresh solvent.

Procedure1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc®. Sandwich the G1 disc between the two C9 discs.2. Weigh the sorbent into the Q-Cup. Spread evenly across the base of the Q-Cup by gentle tapping.3. Weigh the sample into the Q-Cup layering on top of the sorbent. No mixing is needed.

SolventsOptions:Dichloromethane(1:1) Hexane/Acetone

SorbentsC18 (0.8 g)

Wash Program

Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)

1 12020 3:001010

Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)

1 Extraction Solvent 15 0:15150

2 Extraction Solvent 15 0:00150

General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.

label116

ExtractionFrom Goldenseal RootFrom Goldenseal Root

Food Testing, NutraceuticalsEDGE

Note: Temperature and hold times may vary depending on the sample and analytes of interest.

Heating Program

Sample Weight5 g

Q-DiscC1

EquipmentEDGE40 mL collection vials - clear

NotesNone

Procedure1. Assemble the Q-Cup® with the Q-Disc®.2. Weigh the sample into the Q-Cup.

SolventsAcetonitrile

SorbentsN/A

Wash Program

Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)

1 10015 1:00105

Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)

1 Acetonitrile 15 0:15150

2 Acetonitrile 15 0:00150

General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.

label117

Extraction of PAHsFrom Infant FormulaFrom Infant Formula

Food Testing, Nutraceuticals, Personal EDGE

Note: Temperature and hold times may vary depending on the sample and analytes of interest.

Heating Program

Sample Weight4 g

Q-DiscC9 + G1 + C9

EquipmentEDGE40 mL collection vials - clear

NotesNone

Procedure1. Assemble the Q-Cup® with the Q-Disc®. Sandwich the G1 disc between two C9 discs.2. Weigh the sample into the Q-Cup.

SolventsCyclohexane

SorbentsN/A

Wash Program

Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)

1 12020 1:00010

Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)

1 Cyclohexane 15 0:30150

2 Cyclohexane 15 0:00150

General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.

label118

Extraction of NutrientsFrom Protein PowderFrom Protein Powder

Food Testing, NutraceuticalsEDGE

Note: Temperature and hold times may vary depending on the sample and analytes of interest.

Heating Program

Sample Weight1 g

Q-DiscC4

EquipmentEDGE40 mL collection vials - clear

NotesNone

Procedure1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc®. 2. Weigh the sand into the Q-Cup. Spread evenly across the base of the Q-Cup by gentle tapping.3. Weigh the sample into the Q-Cup. Gently mix the sample and the sand in the Q-Cup.

Solvents(80:20) Methanol/Water

SorbentsSand (3 g)

Wash Program

Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)

1 15015 0:101010

Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)

1 (80:20) Methanol/Water 15 0:15150

2 (80:20) Methanol/Water 15 0:00150

General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.

label119

Extraction of Total FatFrom Protein PowderFrom Protein Powder

Food Testing, NutraceuticalsEDGE

Note: Temperature and hold times may vary depending on the sample and analytes of interest.

Heating Program

Sample Weight2 g

Q-DiscC1

EquipmentEDGE40 mL collection vials - clear

NotesNone

Procedure1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc®. 2. Weigh the sand into the Q-Cup. Spread evenly across the base of the Q-Cup by gentle tapping.3. Weigh the sample into the Q-Cup. Gently mix the sample and the sand in the Q-Cup.

SolventsPetroleum Ether

SorbentsSand (6 g)

Wash Program

Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)

1 12015 2:00105

Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)

1 Petroleum Ether 15 0:15150

2 Petroleum Ether 15 0:00150

General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.

label120

ExtractionFrom Spirulina AlgaeFrom Spirulina Algae

Food Testing, NutraceuticalsEDGE

Note: Temperature and hold times may vary depending on the sample and analytes of interest.

Heating Program

Sample Weight2 g

Q-DiscC1

EquipmentEDGE40 mL collection vials - clear

NotesNone

Procedure1. Assemble the Q-Cup® with the Q-Disc®.

2. Weigh the sample into the Q-Cup.

SolventsAcetonitrile

SorbentsN/A

Wash Program

Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)

1 10015 1:00105

Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)

1 Acetonitrile 15 0:00150

2 Acetonitrile 15 0:00150

General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.

label121

Extraction of PAHsFrom Yerba MateFrom Yerba Mate

Food Testing, NutraceuticalsEDGE

Note: Temperature and hold times may vary depending on the sample and analytes of interest.

Heating Program

Sample Weight0.5 g

Q-DiscC1

EquipmentEDGE40 mL collection vials - clear

NotesNone

Procedure 1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc®. 2. Weigh the sample into the Q-Cup.

SolventsOptions:(1:1) Hexane/AcetoneDichloromethaneAcetone

SorbentsN/A

Wash Program

Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)

1 120-15015 1:00105

Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)

1 Extraction Solvent 15 0:30150

2 Extraction Solvent 15 0:00150

General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.

label122

Personal Care Products

123

Extraction of PAHsFrom Infant FormulaFrom Infant Formula

Food Testing, Nutraceuticals, Personal EDGE

Note: Temperature and hold times may vary depending on the sample and analytes of interest.

Heating Program

Sample Weight4 g

Q-DiscC9 + G1 + C9

EquipmentEDGE40 mL collection vials - clear

NotesNone

Procedure1. Assemble the Q-Cup® with the Q-Disc®. Sandwich the G1 disc between two C9 discs.2. Weigh the sample into the Q-Cup.

SolventsCyclohexane

SorbentsN/A

Wash Program

Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)

1 12020 1:00010

Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)

1 Cyclohexane 15 0:30150

2 Cyclohexane 15 0:00150

General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.

label124

Extraction ofMouthwashMouthwash

Personal Care (Health and Beauty)EDGE

Note: Temperature and hold times may vary depending on the sample and analytes of interest.

Heating Program

Sample Weight5 g

Q-DiscC9 + G1 + C9

EquipmentEDGE40 mL collection vials - clear

NotesNone

Procedure1. Assemble the Q-Cup® with the Q-Disc®. Sandwich the G1 disc between the two C9 discs.

2. Weigh the sorbent into the Q-Cup. Spread evenly across the base of the Q-Cup by gentle tapping. 3. Weigh the sample into the Q-Cup layering on top of the sorbent. No mixing is needed.

SolventsHexane

SorbentsOptions:Sodium Polyacrylate (5 g)Diatomaceous Earth (5 g)

Wash Program

Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)

1 4020 3:00010

Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)

1 Hexane 15 0:15100

2 Hexane 15 0:00100

General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.

label125

Extraction ofToothpasteToothpaste

Personal Care (Health and Beauty)EDGE

Note: Temperature and hold times may vary depending on the sample and analytes of interest.

Heating Program

Sample Weight5 g

Q-DiscC9 + G1 + C9

EquipmentEDGE40 mL collection vials - clear

Notes*for sand- mix into sample*for Diatamaceous Earth- layer under sample, do not mix.

Procedure1. Assemble the Q-Cup® with the Q-Disc®. Sandwich the G1 disc between the two C9 discs. 2. Weigh the sorbent/sand into the Q-Cup. Spread evenly across the base of the Q-Cup by gentle tapping.3. Weigh the sample into the Q-Cup*.

SolventsHexane

SorbentsOptions:Sand (15 g)Diatamaceous Earth (5 g)

Wash Program

Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)

1 4020 3:00010

Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)

1 Hexane 15 0:1540

2 Hexane 15 0:0040

General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.

label126

Plastics, Polymers, & Oils

127

Extraction ofFilm PowderFilm Powder

Materials Science, Plastics, Polymers, aEDGE

Note: Temperature and hold times may vary depending on the sample and analytes of interest.

Heating Program

Sample Weight1 g

Q-DiscM1 + C9

EquipmentEDGE40 mL collection vials - clear

Notes*Plastics and polymers should only be extracted at temperatures below melting point range.Expansion of the sample during extraction is common during polymer based extraction.

Procedure 1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc. Layer the M1 disc on top of the C9 disc. 2. Weigh the sample into the Q-Cup.

SolventsOptions:CyclohexaneDichloromethane

SorbentsN/A

Wash Program

Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)

1 *40-7015 10:0005

Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)

1 Cyclohexane 15 0:0070

2 Dichloromethane 15 0:1570

General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.

label128

Extraction of AdditivesFrom PETFrom PET

Plastics, Polymers, and OilsEDGE

Note: Temperature and hold times may vary depending on the sample and analytes of interest.

Heating Program

Sample Weight1 g

Q-DiscC1

EquipmentEDGE40 mL collection vials - clear

Notes*Plastics and polymers should only be extracted at temperatures below melting point range.Expansion of the sample during extraction is common during polymer based extraction.

Procedure 1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc®. 2. Weigh the sample into the Q-Cup.

Solvents(40:60) Acetone/Hexane

SorbentsN/A

Wash Program

Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)

1 115*25 5:00510

Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)

1 (40:60) Acetone/Hexane 15 0:15150

2 (40:60) Acetone/Hexane 15 0:00150

General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.

label129

Extraction of AdditivesFrom PigmentFrom Pigment

Plastics, Polymers, and OilsEDGE

Note: Temperature and hold times may vary depending on the sample and analytes of interest.

Heating Program

Sample Weight1 g

Q-DiscC1

EquipmentEDGE40 mL collection vials - clear

NotesNone

Procedure 1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc®. 2. Weigh the sample into the Q-Cup.

SolventsDimethylformamide

SorbentsN/A

Wash Program

Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)

1 10020 1:001010

Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)

1 Dimethylformamide 10 0:15125

2 Dimethylformamide 10 0:00125

General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.

label130

Extraction of PigmentsFrom PlasticsFrom Plastics

Materials Science, Plastics, Polymers, aEDGE

Note: Temperature and hold times may vary depending on the sample and analytes of interest.

Heating Program

Sample Weight1 g

Q-DiscM1 + C9

EquipmentEDGE40 mL collection vials - clear

Notes*Plastics and polymers should only be extracted at temperatures below melting point range.Expansion of the sample during extraction is common during polymer based extraction.

Procedure 1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc. Layer the M1 disc on top of the C9 disc. 2. Weigh the sample into the Q-Cup.

SolventsOptions:CyclohexaneDichloromethane

SorbentsN/A

Wash Program

Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)

1 *40-8015 10:0005

Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)

1 Cyclohexane 10 0:1580

2 Dichloromethane 10 0:0080

General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.

label131

Extraction of AdditivesFrom Polyethylene PowderFrom Polyethylene Powder

Plastics, Polymers, and OilsEDGE

Note: Temperature and hold times may vary depending on the sample and analytes of interest.

Heating Program

Sample Weight1 g

Q-DiscM1 + C9

EquipmentEDGE40 mL collection vials - clear

Notes*Plastics and polymers should only be extracted at temperatures below melting point range.Expansion of the sample during extraction is common during polymer based extraction.

Procedure 1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc®. Layer the C9 Q-Disc on top of the M1 Q-Disc. 2. Weigh the sample into the Q-Cup.

SolventsIsopropanol

SorbentsN/A

Wash Program

Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)

1 *9030 15:00010

Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)

1 Isopropanol 15 0:1590

2 Isopropanol 15 0:0090

General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.

label132

Extraction of Chemical ModifierFrom Polyethylene ResinFrom Polyethylene Resin

Plastics, Polymers, and OilsEDGE

Note: Temperature and hold times may vary depending on the sample and analytes of interest.

Heating Program

Sample Weight0.1 g

Q-DiscC1

EquipmentEDGE40 mL collection vials - clear

Notes*Plastics and polymers should only be extracted at temperatures below melting point range.Expansion of the sample during extraction is common during polymer based extraction.

Procedure 1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc®. 2. Weigh the sample into the Q-Cup.

Solvents(40:60) Acetone/Hexane

SorbentsNone

Wash Program

Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)

1 *6020 15:001010

Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)

1 (40:60) Acetone/Hexane 10 0:1560

2 (40:60) Acetone/Hexane 10 0:0060

General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.

label133

Extraction of AdditivesFrom Polypropylene PowderFrom Polypropylene Powder

Materials Science, Plastics, Polymers, aEDGE

Note: Temperature and hold times may vary depending on the sample and analytes of interest.

Heating Program

Sample Weight1 g

Q-DiscC9 + G1 + C9

EquipmentEDGE40 mL collection vials - clear

Notes*Plastics and polymers should only be extracted at temperatures below melting point range.Expansion of the sample during extraction is common during polymer based extraction.

Procedure1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc®. Sandwich the G1 disc between the two C9 discs. 2. Weigh the sample into the Q-Cup.

SolventsIsopropanol

SorbentsN/A

Wash Program

Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)

1 12030 15:00010

Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)

1 Isopropanol 15 0:15150

2 Isopropanol 15 0:00150

General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.

label134

Extraction of PhthalatesFrom Polyvinyl ChlorideFrom Polyvinyl Chloride

Materials Science, Plastics, Polymers, aEDGE

Note: Temperature and hold times may vary depending on the sample and analytes of interest.

Heating Program

Sample Weight1 g

Q-DiscC9 + G1 + C9

EquipmentEDGE40 mL collection vials - clear

Notes*Plastics and polymers should only be extracted at temperatures below melting point range.Expansion of the sample during extraction is common during polymer based extraction.

Procedure 1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc. Sandwich the G disc between 2 C9 discs. 2. Weigh the sample into the Q-Cup

Solvents(1:1) Isopropanol/Cyclohexane

SorbentsN/A

Wash Program

Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)

1 *8020 10:00010

Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)

1 (1:1) Isopropanol/Cyclohexane 10 0:1580

2 (1:1) Isopropanol/Cyclohexane 10 0:0080

General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.

label135

Extraction of Diesel and OilsFrom SoilFrom Soil

Environmental and Regulatory, Plastics,EDGE

Note: Temperature and hold times may vary depending on the sample and analytes of interest.

Heating Program

Sample Weight5 g

Q-DiscC9 + G1 + C9

EquipmentEDGE40 mL collection vials - clear

NotesNone

Procedure1. Assemble the Q-Cup® with the Q-Disc®. Sandwich the G1 disc between the two C9 discs. 2. Weigh the sorbent into the Q-Cup.3. Weigh the sample into the Q-Cup on top of the sorbent. No mixing is required.

SolventsDichloromethane

SorbentsDiatomaceous Earth (1 g)

Wash Program

Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)

1 10020 2:0000

2 10010 2:00010

Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)

1 Dichloromethane 15 0:30150

2 Dichloromethane 15 0:00150

General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.

label136